ライフサイエンスコーパス: real-time quantitative PCR

1 and 2 up-regulated genes) were validated by real time quantitative PCR.

2 iral vector to other organs was evaluated by real time quantitative PCR.

3 group were tested for HHV-6 viremia using a real-time quantitative PCR.

4 Relative MCP-1 mRNA was measured by real-time quantitative PCR.

5 vo treatment in PBMCs from healthy donors by real-time quantitative PCR.

6 elongs to the same cluster, was confirmed by real-time quantitative PCR.

7 cytokine signature was further evaluated by real-time quantitative PCR.

8 1), differential expression was confirmed by real-time quantitative PCR.

9 (promote N-glycan branching) as detected by real-time quantitative PCR.

10 as in CD4+CD8- single-positive thymocytes by real-time quantitative PCR.

11 were confirmed in microdissected samples by real-time quantitative PCR.

12 t and 3 and 7 days later, was analyzed using real-time quantitative PCR.

13 individual infection load then estimated by real-time quantitative PCR.

14 iral RNA copy number, which is quantified by real-time quantitative PCR.

15 of these two methods were then validated by real-time quantitative PCR.

16 ded the peak levels of CCL17, as measured by real-time quantitative PCR.

17 e blood recall assays, HLA-A2 tetramers, and real-time quantitative PCR.

18 in lung tissue of bleomycin-treated mice by real-time quantitative PCR.

19 expression were subsequently confirmed using real-time quantitative PCR.

20 The load of infection was then estimated by real-time quantitative PCR.

21 communities in Tanzania and The Gambia with real-time quantitative PCR.

22 ome fetuses and five independent controls by real-time quantitative PCR.

23 sion of some of these genes was confirmed by real-time quantitative PCR.

24 anglia, as demonstrated by virus culture and real-time quantitative PCR.

25 as determined via flow cytometry, ELISA, and real-time quantitative PCR.

26 stigated in situ by immunohistochemistry and real-time quantitative PCR.

27 ved interferons on LAD2 MCs were examined by real-time quantitative PCR.

28 PTGS2, CXCR1, and PTK2 were determined using real-time quantitative PCR.

29 l and oropharyngeal swabs were quantified by real-time quantitative PCR.

30 eletal muscle, and intestine was measured by real-time quantitative PCR.

31 the viruses were equivalent to those of the real-time quantitative PCR.

32 o piperaquine resistance) was assessed using real-time quantitative PCR.

33 tern blot or harboring HDV RNA detectable by real-time quantitative PCR.

34 MRD was measured by using standardized real-time quantitative PCR.

35 ication in human beta-cells was performed by real-time quantitative PCR.

36 east 10-fold more than the other isoforms by real-time quantitative PCR.

37 remodeling, respectively) was quantified by real-time quantitative PCR.

38 ging, whole-mount fluorescence staining, and real-time quantitative PCR.

39 CCDC134, UBD, and ZIC2 were validated using real-time quantitative PCR.

40 wing abnormal expression were validated with real-time quantitative PCR.

41 All patients were monitored by real-time quantitative PCR.

42 ormances and reaching similar sensitivity as real-time quantitative PCR.

43 ntification of the repaired, CPD-free DNA by real-time quantitative PCR.

44 an innate, immune response was quantified by real-time quantitative PCR.

45 e different cultured cells was validated via real-time quantitative-PCR.

46 cycling, making this method well suited for real-time quantitative PCRs.

47 By the use of real-time quantitative PCR, a 10-fold increase in MT-1 a

48 We leveraged reverse transcription and real-time quantitative PCR along with key control experi

49 antified by dilution to single molecules and real-time quantitative PCR amplification.

50 Real time quantitative PCR analyses showed that unlike i

51 Real-time quantitative PCR analyses of Jurkat cell infec

52 In this paper, we report extensive real-time quantitative PCR analyses of SIX3 and SIX6 exp

53 Real-time quantitative PCR analyses showed that the expr

54 at are impaired in the resistant cell lines, real-time quantitative PCR analyses were employed and tw

55 Real time quantitative PCR analysis demonstrates that th

56 Real-time quantitative PCR analysis demonstrates that th

57 Real-time quantitative PCR analysis demonstrates TuSp1 i

58 Real-time quantitative PCR analysis indicated that sever

59 Real-time quantitative PCR analysis of a set of cancer-r

60 Real-time quantitative PCR analysis of gene expression r

61 nimal residual disease level was measured by real-time quantitative PCR analysis of immunoglobulin an

62 RNA-sequencing and real-time quantitative PCR analysis revealed increased g

63 However, our real-time quantitative PCR analysis revealed significant

64 Real-time quantitative PCR analysis revealed that 5azaD/

65 Real-time quantitative PCR analysis shows that PDEs 9A5

66 enome hybridization (aCGH) followed by rapid real-time quantitative PCR analysis to identify, confirm

67 Using microarrays and real-time quantitative PCR analysis, we defined differen

68 dition, the TRAP assay has been modified for real-time, quantitative PCR analysis.

69 as carried out on a subset of 66 clones with real time quantitative PCR and 40 clones were positive.

70 Real time quantitative PCR and Western blot analyses wer

71 seI and Trap1 mRNA levels were determined by real-time quantitative PCR and compared with protein exp

72 cted at enrolment for pneumococci using lytA real-time quantitative PCR and conducted molecular serot

73 Using real-time quantitative PCR and cotransfection reporter a

74 We assessed relative mean TL by real-time quantitative PCR and DNA methylation by Infini

75 face proteins in mast cells was validated by real-time quantitative PCR and flow cytometry analysis i

76 sion varied considerably as measured by both real-time quantitative PCR and flow cytometry analysis.

77 ling using microarray analysis combined with real-time quantitative PCR and flow cytometry.

78 re highly upregulated or downregulated using real-time quantitative PCR and found a strong correlatio

79 The increase was corroborated by real-time quantitative PCR and immunofluorescence.

80 TJ expression was evaluated by using real-time quantitative PCR and immunofluorescence.

81 Using real-time quantitative PCR and immunohistochemical metho

82 vo gene expression profiles were verified by real-time quantitative PCR and immunohistochemistry.

83 Real-time quantitative PCR and in situ hybridization ana

84 Real-time quantitative PCR and in situ hybridization val

85 Using real-time quantitative PCR and in situ hybridization, we

86 Validating these findings by real-time quantitative PCR and layered protein scanning,

87 Real-time quantitative PCR and Northern analyses reveale

88 We have determined, by real-time quantitative PCR and RNase protection, that th

89 of candidate genes by reverse transcriptase real-time quantitative PCR and subsequent testing for ak

90 expression patterns of mouse macrophages by real-time quantitative PCR and tested the functional eff

91 Real-time quantitative PCR and Western blot analysis dem

92 On the basis of real-time quantitative PCR and Western blot analysis, ex

93 irmed in splenocytes and total thymocytes by real-time quantitative PCR and Western blot as well as i

94 human epidermal keratinocytes (n = 11) with real-time quantitative PCR and Western blotting revealed

95 genes in the region was carried out by using real-time quantitative PCR and/or oligo-microarray profi

96 n and gene transcript levels (gene array and real-time quantitative PCR) and compared with insulin-tr

97 n of IL-13Ralpha2 mRNA was measured by using real-time quantitative PCR, and cell-surface IL-13Ralpha

98 TSLP was quantified by ELISA, real-time quantitative PCR, and immunofluorescence stain

99 ds for islet expansion using immunostaining, real-time quantitative PCR, and microarrays at the follo

100 blotting, enzyme-linked immunosorbent assay, real-time quantitative PCR, and RNA-Seq techniques were

101 Our in situ hybridization, real-time quantitative PCR, and stage-specific transcrip

102 mutant TIP30, by Affymetrix GeneChip array, real-time quantitative PCR, and Western blotting assays

103 cts has been utilized extensively in various real-time quantitative PCR applications, including post-

104 assessment of TGF-beta signaling pathways by real-time quantitative PCR array, supported the hypothes

105 g conventional reverse transcriptase-PCR and real-time quantitative PCR, as well as whole-mount in si

106 bjects from 3 independent cohorts by using a real-time quantitative PCR assay to detect a noninherite

107 growth factor (EGF) mRNA were measured using real-time quantitative PCR assay, and levels were correl

108 yzed mRNA expression with a highly sensitive real-time quantitative PCR assay.

109 nd by infected ticks as evaluated by using a real-time quantitative PCR assay.

110 were tested for HIV shedding and VL using a real-time quantitative PCR assay.

111 gand evolution was assured by a differential real-time quantitative PCR assay.

112 in 18 biopsies (10 IFTA and 8 normal) using real-time quantitative PCR assays (RT-QPCR).

113 estimate infection prevalence, we developed real-time quantitative PCR assays for each virus and scr

114 Sensitive qualitative and real-time quantitative PCR assays revealed a higher prev

115 Using real-time quantitative PCR assays to study SXT excision

116 Results of real-time quantitative PCR assays, however, indicated th

117 Real-time quantitative PCR assays, immunohistochemistry,

118 Using real-time quantitative PCR assays, mRNA levels of ERalph

119 using traditional plaque assays, as well as real-time quantitative PCR-based genome quantification a

120 sured by a highly sensitive and quantitative real-time quantitative-PCR-based telomeric repeat amplif

121 lymphocytes' telomere length was measured by real-time quantitative PCR before HSCT.

122 ELISA, real-time quantitative PCR, coculture, flow cytometry, a

123 istry, Western blotting, DNA binding assays, real-time quantitative PCR, coimmunoprecipitation, and E

124 nd associated cytokines was also detected by real-time quantitative PCR comparing lesional with peril

125 Real Time quantitative PCR confirmed that gene expressio

126 Real time quantitative PCR confirmed up-regulation of Eg

127 Real-time quantitative PCR confirmed expression patterns

128 Real-time quantitative PCR confirmed that CpG ODN decrea

129 Real-time quantitative PCR confirmed that IL9 was the hi

130 t of symptoms (POS) from 65 individuals with real-time quantitative PCR-confirmed SARS-CoV-2 infectio

131 Real time quantitative PCR demonstrated an increase in F

132 Real-time quantitative PCR demonstrated that RNA5SP tran

133 More sensitive real-time quantitative PCR detected AID transcripts in v

134 Real-time quantitative PCR detected mRNAs for CD8(+) T-c

135 principle, we demonstrate an electrochemical real-time quantitative PCR (e-PCR) measurement in the to

136 of stellate cells with NETs was measured by real-time quantitative PCR, ELISA, and FACS.

137 microscopy, cocultures, suppression assays, real-time quantitative PCR, ELISAs, and ELISpot assays w

138 portions were subjected to mRNA extraction, real-time quantitative PCR, enzyme-linked immunosorbent

139 "Minimum Information for the publication of real-time Quantitative PCR Experiments" (MIQE) guideline

140 The allelic variation has been confirmed by real-time quantitative PCR experiments.

141 t cell lines and human primary mast cells by real-time quantitative PCR, flow cytometry, and confocal

142 pression of candidate genes was confirmed by real-time quantitative PCR, fluorescence, and immunohist

143 Plaque and saliva samples were tested with real-time quantitative PCR for DNA levels of pathogens r

144 ra-1 gene silenced RPM cells were assayed by real-time quantitative PCR for the expression of possibl

145 Using real-time quantitative PCR for the quantification of gen

146 ce and levels of male DNA were determined by real-time quantitative PCR for the Y chromosome-specific

147 Using immunohistochemistry, real-time quantitative PCR, immunoblotting, ELISA, siRNA

148 Using real-time quantitative PCR, immunofluorescence, Western

149 Microarray findings were confirmed by using real-time quantitative PCR in 30 subjects (12 children w

150 ssion of plasma miRNAs was measured by using real-time quantitative PCR in 35 asthmatic patients, 25

151 ntibody+), and control pancreas tissues with real-time quantitative PCR in accordance with the Minimu

152 chromosome-specific DYS14 gene, we performed real-time quantitative PCR in autopsied brain from women

153 erated, and their expression was measured by real-time quantitative PCR in blood samples from 81 meta

154 aggression testing at both time points using real-time quantitative PCR in brain regions previously i

155 IL-34 mRNA levels were quantified by real-time quantitative PCR in human synovial fibroblasts

156 expression of all known chemokine ligands by real-time quantitative PCR in isolated islets.

157 ional Scale (BCR-ABL(IS)) of 0.1% or less by real-time quantitative PCR in peripheral blood.

158 We analyzed XDH1 and XDH2 gene expression by real-time quantitative PCR in tissues from sugar- and bl

159 We used real-time quantitative PCR, in situ nucleic acid hybridi

160 Analysis of gene expression by real time quantitative PCR indicated that whitefly morta

161 genes using microarray, immunoblotting, and real-time quantitative PCR indicated that PIERCE1 negati

162 Gene expression analyses by real-time quantitative PCR indicated that Smurf2-deficie

163 Real-time quantitative PCR is extremely accurate and les

164 Real-time quantitative PCR is used routinely for the hig

165 Real-time quantitative PCR may be useful in differentiat

166 Real-time quantitative PCR measured CSF2 and CSF2R mRNA

167 ects of this deficiency were demonstrated by real-time quantitative PCR measurements of altered mRNA

168 We have developed a novel "real time" quantitative PCR method.

169 veraged single-cell and bulk RNA sequencing, real-time quantitative PCR, multiplex immunofluorescence

170 ipitation, promoter mutational analysis, and real-time quantitative PCR, NRF-1 was found to functiona

171 immunoprecipitation, promoter mutations, and real-time quantitative PCR, NRF-1 was found to functiona

172 orts to standardize MRD quantification using real-time quantitative PCR of clonal immunoglobulin and

173 ical rates in cell culture, as determined by real-time quantitative PCR of viral particles released i

174 F-beta, IL-5, and IL-10 mRNA was measured by real-time quantitative PCR on tissue homogenates of pati

175 sence or absence of certain antibiotics with real-time quantitative PCR or digital PCR to determine a

176 hondrial DNA, and cytokines were assessed by real-time quantitative PCR or ELISA tests.

177 e tissue using Western blot, flow cytometry, real-time quantitative PCR, or RNA sequencing analyses.

178 itionally, we applied a recently established real-time quantitative PCR platform to gain insight into

179 Next generation RNA sequencing of mRNAs and real time quantitative PCR profiling established that Ez

180 Real-time quantitative PCR provided a sensitive and reli

181 We evaluate here the use of real-time quantitative PCR (q-PCR) as a method for scree

182 genes sirtuins in DDQ-treated tau mice using real-time quantitative PCR (q-RT-PCR), immunoblotting an

183 Using real time quantitative PCR (qPCR) for detection of the b

184 Expression of IL-17 was determined by real-time quantitative PCR (qPCR) after 5 h and protein

185 ta combining transcriptional lacZ fusion and real-time quantitative PCR (qPCR) analyses indicated tha

186 ive genomic hybridization (CGH) database and real-time quantitative PCR (qPCR) analyses to identify t

187 Here we describe a novel real-time quantitative PCR (qPCR) approach based on the

188 We report the first real-time quantitative PCR (qPCR) assay targeting Asperg

189 rformance of the Xpert Carba-R test, a rapid real-time quantitative PCR (qPCR) assay that detects fiv

190 We designed a multiplex real-time quantitative PCR (qPCR) assay to detect SFGR,

191 etitive element, RLPM, upon which a specific real-time quantitative PCR (qPCR) assay was developed.

192 To this end, we developed type-specific real-time quantitative PCR (qPCR) assays for HAdV types

193 We developed two real-time quantitative PCR (qPCR) assays, targeting the

194 Real-time quantitative PCR (qPCR) data were collected fr

195 stochemistry and F4/80 staining coupled with real-time quantitative PCR (qPCR) for interleukin (IL)-1

196 ve higher precision and reproducibility than real-time quantitative PCR (qPCR) in quantifying low lev

197 Real-time quantitative PCR (qPCR) is one of the most pow

198 riance across the lake as fluorescence based real-time quantitative PCR (qPCR) measurements of microc

199 Interestingly, real-time quantitative PCR (qPCR) on E2F2 ChIPs indicate

200 We performed real-time quantitative PCR (qPCR) to determine the abund

201 Taqman real-time quantitative PCR (qPCR) was also compared, bei

202 Real-time quantitative PCR (qPCR) was used to measure mR

203 When measured by comparative real-time quantitative PCR (qPCR), HP1512 transcription

204 ples whose parasite burdens were measured by real-time quantitative PCR (qPCR).

205 ucleotides hybridized to a miRNA followed by real-time quantitative PCR (qPCR).

206 and a greater resistance to inhibitors than real-time quantitative PCR (qPCR).

207 0 min with a walk-away workflow identical to real-time quantitative PCR (qPCR).

208 r the molecular detection of the organism by real-time quantitative PCRs (qPCRs) targeting the uracil

209 ononuclear cells was screened by nested- and real time-quantitative PCR (QRT-PCR) for the presence of

210 th T (porA) than in the one without T (porA) Real-time quantitative PCR (qRT-PCR) analysis of the por

211 chain reaction (ddPCR) is rapidly replacing real-time quantitative PCR (qRT-PCR) as an efficient met

212 Real-time quantitative PCR (qRT-PCR) confirmed the resul

213 Using SnoRNASeq and real-time quantitative PCR (qRT-PCR) we demonstrate snoR

214 atterns of the selected genes were tested by real-time quantitative PCR (qRT-PCR), and the qRT-PCR re

215 Real-time quantitative PCR (qRT-PCR), flow cytometry, an

216 nin-concentrating hormone, as revealed using real-time quantitative PCR, radiolabeled in situ hybridi

217 tification of the transcripts by RNA-Seq and real time quantitative PCR revealed that the CYP3A4 tran

218 The use of cDNA microarrays and real-time quantitative PCR revealed increased expression

219 ypothalamic peptides via radioimmunoassay or real-time quantitative PCR revealed markedly enhanced ga

220 Real-time quantitative PCR revealed no transcripts in ne

221 Real-time quantitative PCR revealed that 96% of the ampl

222 - and hyperoxia-exposed samples were made by real-time quantitative PCR, RNA in situ hybridization, q

223 Progress includes establishment of optimized real-time quantitative PCR (RQ-PCR) assays for WT1 (comm

224 now possible using updated methods including real-time quantitative PCR (RQ-PCR) for abnormal fusion

225 of older patients with NASH, as assessed by real time quantitative PCR (RT-qPCR) or western blots.

226 before and after the 12-week treatment using real-time quantitative PCR (RT-qPCR) analysis.

227 differential abundances were validated using real-time quantitative PCR (RT-qPCR) and Northern blotti

228 chanism of how Dam and GidA influence Act, a real-time quantitative PCR (RT-qPCR) assay was performed

229 ft biopsies were quantified using customized real-time quantitative PCR (RT-qPCR) assays: 30 biopsy-m

230 Real-time quantitative PCR (RT-qPCR) experiments determi

231 detected by RNA-seq or reverse transcription-real-time quantitative PCR (RT-qPCR) in whole-blood samp

232 Western blot, real-time quantitative PCR (RT-qPCR), and transcriptiona

233 oter cloning, and site-directed mutagenesis, real-time quantitative PCR (RT-qPCR), and Western blotti

234 expression on prostate cancer cell lines by real-time quantitative PCR (RT-qPCR), flow cytometry, an

235 Using reverse transcription-PCR (RT-PCR), real-time quantitative PCR (RT-qPCR), RNA-sequencing, an

236 Real-time quantitative PCR (RT-qPCR), single-molecule in

237 ne expression analysis was carried out using real-time quantitative PCR (RT-qPCR).

238 el was examined in 27 fresh PT samples using Real-time quantitative PCR (RT-qPCR).

239 = 14) and in 7 healthy male volunteers using real-time quantitative PCR (RT-qPCR).

240 CR) and AUC, 0.996 (95% CI, 0.993-0.998) for real-time quantitative PCR (RTQ-PCR) (P = .02).

241 Real-time quantitative PCR (RTQ-PCR) provides a quick, a

242 mples were tested for cytomegalovirus DNA by real-time quantitative PCR (rtqPCR).

243 Real-time quantitative PCR showed that BAC-IN84/Ep was d

244 ern blot data, reverse transcription-PCR and real-time quantitative PCR showed that gp340 transcripts

245 Real-time quantitative PCR shows that Afralea3m mRNA is

246 In real-time quantitative PCR studies using absolute plasmi

247 Using gene chip analysis and the real-time quantitative PCR system, we measured transcrip

248 Real-time quantitative PCR systems (Q-PCR) for the rapid

249 Real-time quantitative PCR techniques were used to deter

250 We develop a real-time quantitative PCR telomeric repeat amplificatio

251 Real-time quantitative PCR tests showed elevated levels

252 study, we demonstrate by deep sequencing and real-time quantitative PCR that hepatic levels of Foxa2

253 We developed real-time quantitative PCRs that allow reliable differen

254 By using real-time quantitative PCR, the population dynamics and

255 viral loads of exposed fish, measured using real-time quantitative PCR; the most virulent viral stra

256 to assess metabolic reactions and fluxes and real time quantitative PCR to determine gene expression.

257 We used real-time quantitative PCR to analyze mtDNA integrity, d

258 robotics, multiparameter flow cytometry, and real-time quantitative PCR to analyze T cell activation

259 Using real-time quantitative PCR to detect endogenous GPNMB ex

260 tions of limbal epithelium were subjected to real-time quantitative PCR to determine c-kit ligand exp

261 rain, grade I, II, III, IV) were analyzed by real-time quantitative PCR to determine the expression l

262 der these conditions in future studies using real-time quantitative PCR to evaluate gene expression i

263 ne amplification with message level, we used real-time quantitative PCR to measure hTERT mRNA in 50 e

264 We used real-time quantitative PCR to measure Treponema pallidum

265 -denaturing gradient gel electrophoresis and real-time quantitative PCR to monitor and quantify chang

266 opy, semithin sectioning of leaf tissue, and real-time quantitative PCR to study structural and quant

267 Real-time quantitative PCR using DNA from the animal who

268 e, 14 human bladder tumours were analysed by real-time quantitative PCR using gene-specific primers f

269 array results with the reverse transcription real-time quantitative PCR, using a larger number of non

270 Real Time-quantitative PCR was performed for SERPINA3 tr

271 Real-time quantitative PCR was also used to measure the

272 Real-time quantitative PCR was performed to assess cytok

273 production was measured by using ELISA, and real-time quantitative PCR was performed to detect PGE2

274 Real-time quantitative PCR was performed to determine th

275 Real-time quantitative PCR was used to analyze the expre

276 Real-time quantitative PCR was used to quantitate LYN, S

277 In addition, real-time-quantitative PCR was utilized to assess mRNA l

278 Using RT(2) Profiler PCR Array and real-time quantitative PCR we found several important sy

279 Then, using real-time quantitative PCR we found that PROX1 displays

280 Using real-time quantitative PCR, we demonstrated that several

281 Using real-time quantitative PCR, we establish that hypothalam

282 Using immunocytochemistry and real-time quantitative PCR, we found that winning fights

283 Using real-time quantitative PCR, we found that within 1 h of

284 te and sensitive assay of parasite genotype, real-time quantitative PCR, we have investigated protect

285 Using DNA microarrays and real-time quantitative PCR, we identified genes transcri

286 Using ELISA as well as real-time quantitative PCR, we show that cultured primar

287 Using allele-specific real-time quantitative PCR, we tested the hypotheses tha

288 Microtomography, histology, and real-time quantitative PCR were performed to analyze bon

289 , and mRNA expression levels (measured using real-time quantitative-PCR) were related to fetal and ne

290 tissue samples from lung cancer patients by real-time quantitative PCR, Western blot, and IHC and fo

291 ression was analyzed in CCA cell cultures by real-time quantitative PCR, western blot, and immunofluo

292 changes of gene expression were confirmed by real-time quantitative PCR, Western blotting, and functi

293 This finding was confirmed by real-time quantitative PCR, which also demonstrated an a

294 ion of these genes, 7 of them were tested by real-time quantitative PCR, which verified that they wer

295 Real time quantitative PCR with Mesa Green, targeted at

296 n reaction (PCR) enzymes were compared using real-time quantitative PCR with SYBR Green I detection.

297 The assay combines kinetic (real-time quantitative) PCR with allele-specific amplifi