ライフサイエンスコーパス: recombinant virus

1 a molecular clone, and recovered a wild-type recombinant virus.

2 nor effect on the virulence of the resulting recombinant virus.

3 d as well as wild-type L in the context of a recombinant virus.

4 s reactivated the WT parent but not the R111 recombinant virus.

5 epidemic from another swine influenza A H1N1 recombinant virus.

6 G15 antagonist function and yielded a viable recombinant virus.

7 se genetics, we generated a ToV-PLP knockout recombinant virus.

8 ed A774wt) were used to construct a panel of recombinant viruses.

9 ed fusion activity and engineered these into recombinant viruses.

10 This holds when H3 or H5 replaces H1 in recombinant viruses.

11 priming in mouse CMV (MCMV) infection using recombinant viruses.

12 d or clonal envelopes were used to construct recombinant viruses.

13 ation in mice was analyzed using a series of recombinant viruses.

14 ination with transcomplementation assays and recombinant viruses.

15 ored polypeptide synthesis from plasmids and recombinant viruses.

16 sion, we have successfully constructed three recombinant viruses.

17 two HA mutations were analyzed by generating recombinant viruses.

18 Passage of one recombinant virus, A117F, identified a second site suppr

19 Inducible expression of a recombinant virus-activated transcription factor resulte

20 These recombinant viruses allowed the identification of the re

21 This recombinant virus also provided protection against letha

22 Here we report an HTLV-1 recombinant virus among infected individuals in North Af

23 such an event affects the host range of the recombinant virus and can lead to the creation of novel

24 re-derived an infectious full-length SHC014 recombinant virus and demonstrate robust viral replicati

25 and their epitopes were finely mapped using recombinant viruses and alanine scan mutation array tech

26 We also generated recombinant viruses and analyzed their infections in cel

27 e determinants of the host range, infectious recombinant viruses and chimeras of a genotype 1 isolate

28 eraction was detected on virions produced by recombinant viruses and correlated with reduced target c

29 th in the context of infection studies using recombinant viruses and in ectopic expression experiment

30 Combining fluorescent protein expressing recombinant viruses and multimodal, macroscopic and micr

31 In this study, using recombinant viruses and primary human cells, we show tha

32 )pdm09 and A/Switzerland/9715293/2013 (H3N2) recombinant viruses and their I38T PA mutants were compa

33 lasmids, which can be used for the rescue of recombinant viruses and/or the creation of vaccine seed

34 rmed the associated molecular changes in the recombinant viruses, and sequence analysis demonstrated

35 We propose that these recombinant viruses are convenient and valuable tools fo

36 Recombinant viruses are genetically stable and induce po

37 Recombinant viruses are rare in the initial rebound viru

38 Recombinant viruses are the workhorse of modern neurosci

39 Recombinant virus ASFV-G-DeltaMGF effectively confers pr

40 Here we report the construction of a recombinant virus (ASFV-G-DeltaMGF) derived from the hig

41 t the construction of a double-gene-deletion recombinant virus, ASFV-G-Delta9GL/DeltaUK.

42 sequences of K3 or K5 into a DeltaK3 DeltaK5 recombinant virus, at either original or interchanged ge

43 aluation of Gag function in the context of a recombinant virus backbone.

44 Mutation of the HD of IBV E in a recombinant virus background results in impaired growth

45 idation of an algorithm capable of detecting recombinant viruses based on diagnostic microarray hybri

46 In vivo, MuHV-4 recombinant viruses bearing these mLANA SOCS box mutatio

47 y retained the attenuation properties of the recombinant virus but enhanced the expression level of H

48 nd BJAB cells with wild-type and the K8-null recombinant viruses by introducing the cloned viral geno

49 esigned to determine if this CD80-expressing recombinant virus can restore all LAT functions as obser

50 lization approach in which we fed virions or recombinant virus capsid components to whiteflies, follo

51 We showed previously that a recombinant virus carrying a deletion of the carboxyl-te

52 Studies with recombinant viruses carrying mutations in this region co

53 Recombinant viruses carrying YNND mutations in AD-5 were

54 Immunization of mice with this recombinant virus conferred complete protection from let

55 Vaccination of chickens with these recombinant viruses conferred complete protection agains

56 f specific-pathogen-free chickens with these recombinant viruses conferred significant protection aga

57 and neurovirulence by generating a series of recombinant viruses consisting of combinations of genes

58 opic) viruses was identified in a library of recombinant viruses constructed with individual envelope

59 Recombinant virus containing a mutation at the T286 posi

60 The recombinant virus containing all three mutations, gDDelt

61 We generated HTLV-1 envelope recombinant virus containing the HTLV-2 SU domain.

62 A recombinant virus containing the P-T101A mutation (rMuV-

63 T lacks ATP-dependent excision activity, and recombinant virus containing this RT remains susceptible

64 f wild-type and laboratory-adapted MVs using recombinant viruses containing an additional transcripti

65 on antigenicity, we constructed a series of recombinant viruses containing different mutation combin

66 Recombinant viruses containing each individual or combin

67 and T212I, were characterized by generating recombinant viruses containing either one or both amino

68 Recombinant viruses containing full-length plasma-derive

69 Here we analyzed recombinant viruses containing HA with exchange of conse

70 if these mutations affect virus replication, recombinant viruses containing single-amino-acid substit

71 Recombinant viruses containing these mutations were then

72 Recombinant viruses currently enable some combination of

73 The recombinant virus DC474-480 constructed with tyrosines 4

74 the role of UL37 in virion envelopment, the recombinant virus DC480 was constructed by insertion of

75 rfaces was enhanced in cells infected with a recombinant virus defective in 14-3-3 binding.

76 al or greater protection than rXlIFN against recombinant viruses deficient for the putative immune ev

77 Infection of A549 cells with recombinant viruses deficient in the expression of NS1 a

78 The recombinant virus DeltagM2, engineered not to express gM

79 The recombinant virus DeltaUL11-DeltagM2, engineered not to

80 We constructed a recombinant virus (DeltaXX) that lacks amino acids 87 to

81 could not allow for the generation of viable recombinant viruses, demonstrating that these residues a

82 Using a recombinant virus derived from the JFH1 strain, we confi

83 Using reverse genetics, we generated a recombinant virus, designated r2segMP12, containing a tw

84 ence of MHV-A59, and mice infected with this recombinant virus developed pulmonary lesions that were

85 This recombinant virus, E1DeltaCys24/94v, showed delayed grow

86 Importantly, these recombinant viruses elicited high levels of neutralizing

87 Here using a recombinant virus encoding a NS1B protein defective in I

88 A recombinant virus encoding an M protein with seven lysin

89 P13/14 from lysates of cells infected with a recombinant virus encoding His-tagged pU(L)17.

90 Recombinant virus encoding the NSs core domain induced i

91 ORF showed that it was possible to generate recombinant viruses encoding 2 heterologous proteins (mR

92 Recombinant viruses encoding amino acid changes in the H

93 nsmission, the replication capacities of 148 recombinant viruses encoding plasma-derived Gag-protease

94 ess, we utilized reverse genetics to produce recombinant viruses encoding wild-type M1 41P (rSPN04-P)

95 These recombinant viruses expressed two additional copies of t

96 ng viral DNA in infected cells, we created a recombinant virus expressing a FLAG-tagged version of UL

97 -specific CD8(+) T cells or vaccination with recombinant virus expressing an MHC I-restricted Chlamyd

98 Recently, we reported that a similar recombinant virus expressing CD80 in place of LAT had hi

99 However, HSV-1 recombinant virus expressing cpIAP did not restore all L

100 A recombinant virus expressing E1086A D1087A mutant ICP8 w

101 Furthermore, a recombinant virus expressing H7N9 NS1-I106M replicates t

102 Recombinant virus expressing pHA-UL3825-331 replicated w

103 Recombinant viruses expressing A34 with a full, partial,

104 rstand the function of this region, a set of recombinant viruses expressing A34 with the full, partia

105 by tandem-affinity purification (TAP) using recombinant viruses expressing either a full-length NTAP

106 ns in modulating DC maturation by generating recombinant viruses expressing enhanced green fluorescen

107 We generated recombinant viruses expressing G and F, or null for G, f

108 ection with IL-12p35 or IL-12p40 DNA or with recombinant viruses expressing IL-12p35 or IL-12p40.

109 changes in the optic nerve and CNS, whereas recombinant viruses expressing IL-4, gamma interferon, I

110 ty of a MERS-CoV molecular clone, as well as recombinant viruses expressing indicator proteins, will

111 of a LAT-minus [LAT(-)] virus, while similar recombinant viruses expressing interleukin-4 (IL-4) or i

112 Finally, recombinant viruses expressing MIBE or SSPE fusion compl

113 Here, we used recombinant viruses expressing mutant NS1 from the A/Tex

114 Moreover, recombinant viruses expressing O3 homologs in place of O

115 y and DNA packaging, we isolated a number of recombinant viruses expressing pUL25, pUL17, and pUL36 f

116 d vIL-6 variants and the lytic phenotypes of recombinant viruses expressing selected variants.

117 Recombinant viruses expressing subtype C Gag-proteases e

118 nfected with mouse cytomegalovirus (MCMV) or recombinant viruses expressing the viral m157 glycoprote

119 Both B5 and B5-GFP recombinant viruses expressing these mutant proteins in

120 e also observed by utilizing three different recombinant viruses expressing unique fluorescent report

121 Using a recombinant virus-expression system in tissue culture an

122 lar clones, as well as for the generation of recombinant virus from the molecular clones.

123 We then generated recombinant viruses from cloned cDNAs prepared to the an

124 ved with a previously described panel of six recombinant viruses from five different subtypes.

125 In contrast, recombinant viruses from which the VNDT motif is deleted

126 The recombinant virus gDDeltaTEV was engineered to eliminate

127 Deletion of the E2RE in the context of a recombinant virus greatly diminished levels of Cp-initia

128 Interestingly, one of the recombinant viruses had a 34-amino-acid duplication at t

129 These recombinant viruses had a significant decrease in both g

130 n was nearly absent in cells infected with a recombinant virus harboring an S369A mutation within the

131 We found that a recombinant virus harboring the duplication bound more e

132 use model utilizing Cre recombinase-encoding recombinant viruses harboring deletions of the core LAT

133 Recombinant viruses harboring engineered deletions of sp

134 Here we observed that recombinant viruses harboring individual cysteine-to-ser

135 tein to inhibit general gene expression, and recombinant viruses harboring these mutations were atten

136 osaic influenza B hemagglutinin proteins and recombinant viruses have been generated as novel vaccine

137 Recombinant viruses have pros and cons as vaccine carrie

138 Recombinant viruses having either tyrosine 476 or 477 re

139 Recombinant viruses hold promise as vectors for vaccines

140 We now show that this recombinant virus (HSV-CD80) expressed high levels of CD

141 l lines demonstrated that replication of the recombinant virus, HSV-GS3, is strictly dependent on an

142 Taking advantage of a recombinant virus in which F18 expression is IPTG (isopr

143 Passage of the recombinant viruses in cell culture led to the accumulat

144 We generated a panel of US28 recombinant viruses in the bacterial artificial chromoso

145 fectivity, and growth kinetics of these four recombinant viruses in vitro.

146 and extend the siRNA results, we constructed recombinant viruses in which pUL48 and pUL103 are fused

147 Entry and spread profiles of the recombinant viruses indicated that gD retargeting does n

148 AC prime/NYVAC boost immunization shows that recombinant viruses induced polyfunctional Env-specific

149 Expression of ILTV gB and gD proteins in the recombinant virus-infected cells was detected by immunof

150 hese results suggest that the rLS/AMPV-C F&G recombinant virus is a safe and effective bivalent vacci

151 The recombinant virus is virulent in established ZIKV mouse

152 The replication of these recombinant viruses is attenuated, and they have an enha

153 th pathogen-associated molecular patterns or recombinant viruses is being tested in the clinic.

154 clones and demonstrate that the behavior of recombinant viruses is similar to that of the wild type.

155 Additionally, cells infected with the recombinant viruses KSHVDeltaK1 and KSHV-K15xSTOP also y

156 We report that the recombinant viruses KSHVDeltaK1 and KSHV-K15xSTOP displa

157 Using a recombinant virus lacking PB1-F2, we confirmed that dele

158 That said, the attenuated replication of a recombinant virus lacking residues 68 to 87 (termed Delt

159 We constructed a recombinant virus lacking the sequences for miR-H1-5p an

160 Comparing phenotypes exhibited by the recombinant virus lacking these miRNAs to the wild type

161 vel expression of hNIS is detrimental to the recombinant virus, leading to the aggregation of hNIS pr

162 Infection of C57BL/6 mice with this recombinant virus led, however, to the generation of abu

163 and four distinct Fabs reactive with Norwalk recombinant virus-like particles (rVLPs) were recovered,

164 ction of 25 different norovirus genotypes as recombinant virus-like particles or in clinical samples

165 Thus, these recombinant viruses may be promising vaccine candidates

166 se chimeras are viable and suggest that such recombinant viruses may be useful for investigation of d

167 Together these data suggest this recombinant virus merits further study for its oncolytic

168 Infant-derived Gag-protease NL4-3 recombinant viruses (n = 41) were found to have a signif

169 In this study, we engineered six different recombinant viruses: NDVs expressing checkpoint inhibito

170 irected mutagenesis, we found that a 1918 HA recombinant virus, of high virulence, could be significa

171 characteristics of CIVs, we constructed four recombinant viruses on H3N8 and H3N2 CIV backgrounds bea

172 We generated two recombinant viruses, one in which the nonstructural prot

173 ferrets with beta-propiolactone-inactivated recombinant virus particles elicited protective RABV ant

174 rom clade C and were used as the prime, with recombinant virus plus envelope protein used as the boos

175 These chimeric recombinant viruses possess growth properties similar to

176 ing a reverse genetics approach by comparing recombinant viruses possessing amino acid substitutions

177 Recombinant viruses predominantly expressing GP1,2 are k

178 pe 2 (hPIV2) by a reverse genetics method of recombinant virus production.

179 ed components of the viral polymerase, while recombinant viruses propagated in MDBK cells acquired mu

180 Recombinant viruses propagated in Vero cells acquired mu

181 tro and in vivo, and immunization with these recombinant viruses protected mice against lethal influe

182 the establishment of latency, we constructed recombinant virus (R112) carrying a dominant-negative RE

183 Recombinant virus (rAAV) leptin antagonism in the VTA de

184 The rescued recombinant virus (rAPMV-2) resembled the biological vir

185 onasal or intramuscular challenge, all three recombinant viruses (rAPMV3, rAPMV3-F, and rAPMV3-HN) we

186 Two recombinant viruses, rAPMV3-F and rAPMV3-HN, were genera

187 A recombinant virus, rBUNdelNSs, that is unable to express

188 e tested in vitro; 2 were not due to lack of recombinant virus recovery.

189 Yet the recombinant virus remained completely defective for prod

190 ich otherwise lacks the locus, the resulting recombinant virus replicated similarly to the parental v

191 The recombinant viruses replicated efficiently in both HEp-2

192 Recombinant viruses replicated in an ORF30-complementing

193 The recombinant viruses replicated to near-wild-type titers,

194 All recombinant viruses replicated well in BHK and undiffere

195 The H5N1 PB1-V43I-recombinant virus replicates to comparable titres as the

196 Insertion of the TC tag interfered with recombinant virus rescue in six of the eight mutants, li

197 mination of the N mRNA and were required for recombinant virus rescue.

198 on of Nicotiana benthamiana plants with such recombinant virus resulted in production of huge amounts

199 use bone marrow-derived DCs with each of the recombinant viruses resulted in DC activation, as shown

200 infection of cynomolgus macaques with these recombinant viruses revealed differences in immunogenici

201 Here, we subjected a recombinant virus (rH1N1) with the same constellation ma

202 Cs that were elicited by the IL-7-expressing recombinant virus (rLBNSE-IL-7) were able to sustain VNA

203 The recombinant virus, rLS/AMPV-C F&G, was slightly attenuat

204 These recombinant viruses, rLS/ILTV-gB and rLS/ILTV-gD, were s

205 ed full-length transcripts recovered several recombinant viruses (rMERS-CoV) that contained the expec

206 se outbreaks, we synthetically resurrected a recombinant virus (rSADS-CoV) as well as a derivative en

207 The resultant recombinant viruses (rVSV-HSP70-VP1 and rVSV-Luc-VP1) we

208 s sole entry glycoprotein and show that this recombinant virus, rVSV-SARS-CoV-2 S, closely resembles

209 The recombinant virus showed distinct changes in tissue trop

210 oculated intranasally with any of these live recombinant viruses showed no signs of disease, includin

211 To investigate its role, we created the recombinant virus SMin79, in which pM79 expression was d

212 vestigate the role of pM92, we constructed a recombinant virus SMin92, in which pM92 expression was d

213 d replicative capacity; however, analysis of recombinant viruses suggested that other sequences in En

214 ding of both virus-like particles (VLPs) and recombinant viruses, suggesting that 14-3-3 binding impa

215 ron function.IMPORTANCE We developed a novel recombinant virus that allows the study of cells that su

216 mouse-adapted SARS-CoV-2 (SARS-CoV-2 MA), a recombinant virus that can use mouse ACE2 for entry into

217 the PIV5 wild type (wt) and PIV5-VDeltaC (a recombinant virus that does not encode a functional V pr

218 A recombinant virus that fails to express both pUL133 and

219 Therefore, we generated a recombinant virus that is no longer able to express sGP

220 The recombinant viruses that arise in nature are occasionall

221 Using reverse genetics, we generated recombinant viruses that contained deletions within the

222 the KSHV life cycle, we constructed a set of recombinant viruses that contained either wild-type (WT)

223 in infection, we generated a series of ZIKV recombinant viruses that disrupted the hydrogen-bonding

224 Further, using recombinant viruses that establish a nonreactivating, la

225 We generated recombinant viruses that express a consensus BA G gene o

226 rus reverse genetics (RG) system to generate recombinant viruses that express a separate heterologous

227 ion assay to determine the entry kinetics of recombinant viruses that incorporated full-length VCV-se

228 ibition of viral RNA synthesis by generating recombinant viruses that lack known ISGylation sites in

229 interrupting any of the viral ORFs, yielding recombinant viruses that likely express the complete set

230 Utilizing recombinant viruses that mutate the miRNA-binding site c

231 in in the life cycle of KSHV, we constructed recombinant viruses that were deficient for K1.

232 ed to the production of genetically modified recombinant viruses that, while attenuated, are able to

233 of the viral genome to generate ORF37-Q129H recombinant virus (the Q129H mutant) and investigated th

234 For the V787E recombinant virus, the half maximal effective concentrat

235 en these mutations were engineered back into recombinant viruses, the resulting viruses replicated we

236 VSVDeltaG vector restored the ability of the recombinant virus to replicate in cell culture, without

237 he virion shell can influence the ability of recombinant viruses to cross the vascular barrier, enter

238 e candidate mutations were incorporated into recombinant viruses to determine their in vivo effect.

239 The recombinant virus, UL12 D340E (the D340E mutant), behave

240 We show here that this recombinant virus vaccine candidate was nonpathogenic in

241 iate, but by using the direct infection of a recombinant virus vector based on the plant virus, tobac

242 A recombinant virus, vNC-Venus-ICP27, was constructed, and

243 Three recombinant viruses, vR128A, vR129A, and vRR129AA, carry

244 P in supporting mRNA transcription in vitro Recombinant virus VSV-P(DeltaOD) exhibits a pronounced k

245 The recombinant virus was assembly deficient, as judged by t

246 The VC2-EHV-1-gD recombinant virus was constructed by inserting an EHV-1

247 The 1918 HA recombinant virus was further attenuated by introducing

248 15 production and conjugation, but no viable recombinant virus was recovered.

249 This recombinant virus was unable to establish latency.

250 NSP3 made by the recombinant viruses was functional, inducing nuclear acc

251 The replication of these recombinant viruses was highly attenuated in the upper a

252 Using recombinant virus, we demonstrate for the first time PVM

253 Using these recombinant viruses, we assessed the requirement for US2

254 Using recombinant viruses, we demonstrate that while Cp is not

255 mammalian protein complementation assay and recombinant viruses, we found that an increase in P(XD)-

256 Using recombinant viruses, we identify the HA genomic segment

257 periments revealed that A774wt and avirulent recombinant virus were characterized by increased proces

258 immune responses induced by our panel of IAV recombinant viruses were also characterized in normal hu

259 All recombinant viruses were attenuated both in vitro and in

260 Recombinant viruses were constructed to abolish either g

261 Recombinant viruses were generated that contained either

262 Three recombinant viruses were generated that contained the op

263 In addition, these recombinant viruses were highly attenuated in cell cultu

264 the replication capacities of the resulting recombinant viruses were measured in an HIV-1-inducible

265 hment protein- and fusion protein-expressing recombinant viruses were protected from lethal CDV chall

266 These recombinant viruses were safe, stable, and immunogenic a

267 These recombinant viruses were specifically defective in ribos

268 These recombinant viruses were specifically defective in ribos

269 into the wild-type HSV-1(F) genome, and the recombinant viruses were tested for raltegravir resistan

270 plasticity of the BUNV genome by generating recombinant viruses where the normal negative-sense S se

271 Another recombinant virus, which induced a slowly developing "in

272 ed influenza virus vaccines (LAIVs) based on recombinant viruses whose genomes encode nonoverlapping

273 To test this hypothesis, we used a recombinant virus with a 135-bp deletion spanning only t

274 a bacterial artificial chromosome (BAC) KSHV recombinant virus with a deletion of the RBP-Jkappa site

275 HAdV-C57 is a recombinant virus with a fiber gene nearly identical to

276 wild-type V protein (rBC), (ii) an isogenic recombinant virus with a mutant V protein (rBC-Edit viru

277 Recombinant virus with a nonphosphorylatable alanine (Al

278 e pathogenicity of the virus, we generated a recombinant virus with a single amino acid mutation at t

279 -type RRV(17577) (WT(BAC) RRV) to generate a recombinant virus with all 8 of the vIRFs deleted (vIRF-

280 importance of this residue by engineering a recombinant virus with an S368R point mutation that was

281 In our study, a recombinant virus with seven gene segments from a human

282 uses with reduced inhibition by oseltamivir (recombinant virus with the E119A mutation generated by r

283 Recombinant virus with the PA M285K mutation completely

284 A recombinant virus with these two segments replicated mor

285 vitro assays of viral protein functions and recombinant viruses with defined genetic modifications h

286 e function of pUL33, we generated a panel of recombinant viruses with either deletions or substitutio

287 the UL25 capsid binding domain, we generated recombinant viruses with either small truncations or ami

288 mivir, zanamivir, and peramivir by assessing recombinant viruses with mutant NA-encoding genes (catal

289 Recombinant viruses with only the fusion protein in thei

290 ation and viral pathogenesis, we constructed recombinant viruses with or without mutations within the

291 Here, using recombinant viruses with point mutations that alter the

292 9 virus promoted aerosol transmissibility to recombinant viruses with PR8 and sw/Tx/98 virus backgrou

293 In addition, we isolated recombinant viruses with specific alanine substitutions

294 ation, we infected human DCs with a panel of recombinant viruses with the same backbone (A/Puerto Ric

295 Recombinant viruses with the third-wave NS gene induced

296 Recombinant viruses with these substitutions were genera

297 New alternative strategies for generating recombinant viruses with vaccine potential are needed.

298 hese residues were mutated to alanine, and a recombinant virus, WRL-A, was constructed.

299 In this study, we rescued recombinant virus WSN-AichiM1 containing the spherical A

300 ecies groups have a similar impact on viable recombinant virus yields, which is indicative of conserv