ライフサイエンスコーパス: reconstitution

1 le within grafts and lacked anchoring fibril reconstitution.

2 antiretroviral therapy and CD4 preservation/reconstitution.

3 dspace concentration in < 16 s upon start of reconstitution.

4 nt hematopoietic lineages during bone marrow reconstitution.

5 al trajectories during long-term bone marrow reconstitution.

6 y were used to assess engraftment and immune reconstitution.

7 tive antiretroviral therapy following immune reconstitution.

8 plotypes, CRISPR gene editing and functional reconstitution.

9 compared their biochemical properties after reconstitution.

10 carcinoma LM that is reversible by estradiol reconstitution.

11 after heterologous purification and liposome reconstitution.

12 ted patients on ART with insufficient immune reconstitution.

13 sion and the risk of infection before immune reconstitution.

14 rogression of the disease and improve immune reconstitution.

15 ed for safety, clinical symptoms, and immune reconstitution.

16 ause of the combined effect of T and NK cell reconstitution.

17 that may be partially rescued by microbiome reconstitution.

18 ion between the course of viremia and immune reconstitution.

19 we studied ADAP1's functions using in vitro reconstitutions.

20 Here, we present 'on-gradient' reconstitution, a new gentle approach for the reconstitu

21 atopoietic stem cells (HSC) showed decreased reconstitution ability after serial transplantation.

22 ectional approach and activity-dependent GFP reconstitution across synaptic partners (GRASP) to show

23 increased self-renewing HSC with competitive reconstitution advantages.

24 L-21R attenuated the process of T and B cell reconstitution after adoptive cellular transfer.

25 yeloid engraftment is important for platelet reconstitution after either myeloablative or busulfan-co

26 Because poor B-cell reconstitution after hematopoietic stem cell transplanta

27 rium exchange mass spectrometry, lipoprotein reconstitution, amino acid sequence analysis, and molecu

28 Using protein-RNA reconstitution and a stringent RNA pulldown assay with h

29 In vitro protein reconstitution and associated biochemical/physical studi

30 ation, quantitative imaging, mechanochemical reconstitution and atomic force microscopy, we find that

31 ng IPKSs was investigated using heterologous reconstitution and biochemical studies.

32 e summarize recent progress in computational reconstitution and biologic exploration of CRCs across v

33 Using in vitro reconstitution and cellular assays, we demonstrate that

34 al physiological functions prior to cellular reconstitution and characterizes macrophage-derived WNT

35 -coalescence module (SCM) for the controlled reconstitution and delivery of inkjet-spotted and dried

36 Reconstitution and depletion studies indicate that WRN d

37 analyses, with implications for specificity, reconstitution and evolution.

38 tric liposomes, and it can be concluded that reconstitution and generation of asymmetry were successf

39 Using in vitro reconstitution and in vivo experiments, we demonstrate t

40 n have diminished capacity to mediate T cell reconstitution and initiate heart allograft rejection up

41 suggest that adult HSCs are diverse in their reconstitution and lineage potentials.

42 eficiency in T cells enhanced splenic B-cell reconstitution and reduced follicular T helper cell deve

43 MTC 24 hours post TBI facilitated structural reconstitution and restoration of functional absorption

44 It allows studying VOCs release upon reconstitution and supports the development of novel tec

45 e HIV infection did not recover after immune reconstitution, and a previously unrealized decline in p

46 sualization, substitution, characterization, reconstitution, and simulation.

47 CESA chimeric proteins, in vitro biochemical reconstitution, and supporting computational modeling an

48 To address these questions, we take a reconstitution approach and compare regulated SNARE-medi

49 Here, we use an in vitro reconstitution approach to investigate minus-end dynamic

50 Here, using an in vitro reconstitution approach, we demonstrate that minimal mid

51 By taking a gene-knockout-and-reconstitution approach, we have characterized the antiv

52 a combination of computational and in vitro reconstitution approaches to determine the conditions le

53 umental in guiding the optimal use of immune reconstitution as a durable therapeutic strategy.

54 Furthermore, we combine our in vitro reconstitution assay and egg extract experiments with co

55 In a mitochondrial reconstitution assay, adrenal PMs supported steroidogene

56 TBSV replication using a cell-free replicase reconstitution assay.

57 replicase assembly in a cell-free replicase reconstitution assay.

58 In vivo mouse reconstitution assays resealed that only CD69(High) LSCs

59 Competitive reconstitution assays revealed an approximately sevenfol

60 lico results are validated experimentally by reconstitution assays with actin on lipid bilayer membra

61 Using reconstitution assays with fluorescence readouts, we fou

62 Reconstitution assays with proteoliposomes provide a pow

63 igated whether such asymmetry also occurs in reconstitution assays with the neuronal SNAREs, using sy

64 or activity in organoid formation and tissue reconstitution assays.

65 Epicutaneous sensitization- and bone marrow reconstitution-based models of IgE-mediated food allergy

66 New reconstitution, biochemical and structural studies are r

67 F had no adverse effect on regulatory T-cell reconstitution, but linked adaptive to innate immunity b

68 60 and day 90/100 to evaluate T and NK cells reconstitution by flow cytometry.

69 abrogated disease development, whereas their reconstitution by spleen-derived macrophage transplantat

70 t restore thymic function and enhance T cell reconstitution can provide considerable benefit to indiv

71 tical role in maintaining the quiescence and reconstitution capacity of HSCs and that hypercholestero

72 s effectively restored the LT population and reconstitution capacity of HSCs isolated from Tet1(-/-)

73 ng-term (LT) population, side population and reconstitution capacity of HSCs were significantly decre

74 an agonist partially rescued the number and reconstitution capacity of Sel1l-deficient HSCs.

75 iescence, increased apoptosis, and defective reconstitution capacity.

76 GvHD prophylaxis and overcome delayed T cell reconstitution caused by thymic damage.

77 profile for CMV disease, CMV-specific T-cell reconstitution, CMV viral load, and the potential drug r

78 iated antibiotic tolerance using biochemical reconstitution combined with structural biology and high

79 Giant unilamellar vesicle (GUV) reconstitution confirmed that membrane recruitment by th

80 B lymphocyte reconstitution dominated by memory phenotypes was associ

81 knockout, xenotransplantation, immunological reconstitution, drug-induced, or spontaneous mutation mo

82 e, most likely the result of improved immune reconstitution due to better HIV care and management in

83 wever, several challenges, such as effective reconstitution during inflammation, remain if gene thera

84 Reconstitution enabled genetic experiments refining our

85 In this study, early immune reconstitution, especially of T and NK cell compartments

86 able P conditions, we conducted a microbiota reconstitution experiment.

87 al analysis results, we designed biochemical reconstitution experiments and demonstrated that DNA syn

88 es of plaque vulnerability using bone marrow reconstitution experiments and pharmacological targeting

89 Using in vitro reconstitution experiments and total internal reflection

90 Through loss-of-function and reconstitution experiments in pups, we showed that NK ce

91 Lentiviral shRNA knockdown and reconstitution experiments revealed that both a function

92 Biophysical reconstitution experiments revealed that non-enzymatic a

93 sensitization model was used and bone marrow reconstitution experiments were performed to test the re

94 Here, we combine in vitro reconstitution experiments with computational modeling t

95 these structures, combined with biochemical reconstitution experiments, cross-linking mass spectrome

96 In reconstitution experiments, we showed that human DNA pol

97 ight considerations for designing condensate reconstitution experiments.

98 All 3 showed early autologous T-cell reconstitution following allogeneic hematopoietic stem c

99 However, early phases of hematopoietic reconstitution following bone marrow transplantation pro

100 ness-reducing mutations during hematopoietic reconstitution following bone marrow transplantation.

101 tim) is used clinically to accelerate immune reconstitution following chemotherapy and is being pursu

102 This case demonstrates successful immune reconstitution following hematopoietic stem cell transpl

103 However, delayed immune reconstitution following rATG treatment, partly caused b

104 expanded HSPCs were capable of hematopoietic reconstitution for at least 24 weeks in immunocompromise

105 (conv) and was compensated for by peripheral reconstitution for T(reg) We show that H2-A(j) favors se

106 iving systems that is becoming accessible to reconstitution from purified components, and thus a more

107 geting can be improved by intein-based toxin reconstitution from two dysfunctional fragments provided

108 re key for the antiviral response and immune reconstitution.FUNDINGNIH grant P01 CA23766 and NIH/Nati

109 a high risk of graft failure and poor immune reconstitution have been observed in the absence of myel

110 tic defense gene prediction and heterologous reconstitution, here we discover 29 widespread antiviral

111 tance caused by HR restoration through BRCA1 reconstitution, HR restoration by 53BP1 pathway inactiva

112 to 1 was established by heterologous pathway reconstitution in A. nidulans, which led to biosynthesis

113 function play a role for insufficient immune reconstitution in HIV infection independently of the gut

114 L18-producing cells may interfere with their reconstitution in HIV-1-infected patients on cART.

115 s multilineage engraftment and robust immune reconstitution in mice with either null or hypomorphic R

116 -effective modality to improve hematological reconstitution in patients.

117 antation enabled stable hematopoietic system reconstitution in recipients with mismatches at half (ha

118 caused increased and sustained hematopoietic reconstitution in serial transplantation experiments.

119 and stability of biochemical and functional reconstitution in the progeny of engrafted cells at 12 m

120 WASp reconstitution in vitro and in WAS patients following cl

121 perturbations of blastomere dimensions, and reconstitution in vitro.

122 Reconstitution in yeast (Saccharomyces cerevisiae) prote

123 Furthermore, immune reconstitution included both adoptively transferred clon

124 een the proteins employed in the biochemical reconstitution including a direct interaction between Ra

125 Cellular studies and in vitro reconstitution indicate that hypoxia acts through HIF-in

126 h low CD4 counts are at high risk for immune reconstitution inflammatory syndrome (IRIS) and death at

127 al therapy (ART) are at high risk for immune reconstitution inflammatory syndrome (IRIS) and death.

128 -to-control infections can experience immune reconstitution inflammatory syndrome (IRIS) following en

129 stribution, histoplasmosis-associated immune reconstitution inflammatory syndrome (IRIS) in people li

130 Immune reconstitution inflammatory syndrome (IRIS) occurs in up

131 ractions, overlapping toxicities, and immune reconstitution inflammatory syndrome (IRIS).

132 risk of the paradoxical TB-associated immune reconstitution inflammatory syndrome (TB-IRIS) when they

133 ossible manifestation of tuberculosis immune reconstitution inflammatory syndrome (TB-IRIS).

134 aution is needed in the management of immune reconstitution inflammatory syndrome, an exuberant immun

135 serious infections, serious non-ADE, immune reconstitution inflammatory syndrome, or death).

136 continued to increase until onset of immune reconstitution inflammatory syndrome.

137 e broad spectrum of fungal-associated immune reconstitution inflammatory syndromes.

138 l-free expression, functional refolding, and reconstitution into lipid membranes.

139 ly monomeric in detergent solution and after reconstitution into proteoliposomes, as shown by blue na

140 We present the outcomes, safety and immune reconstitution (IR) of patients with active, treatment r

141 ncouraging results, full immune and platelet reconstitution is not always achieved.

142 mics parallels that of cDC1 and cDC2 in both reconstitution kinetic studies and parabiotic mice.

143 on in Escherichia coli In vitro Fe-S cluster reconstitution led to the insertion of one [4Fe-4S](2+)

144 Peripheral blood chimerism, recipient immune reconstitution, liver function tests, and graft survival

145 eiving hPBMCs showed robust and reproducible reconstitution, mainly by T and B cells.

146 In vitro reconstitutions map the key binding interface to a few a

147 By integrating knockout and reconstitution models with system-wide transcriptomics,

148 Moreover, reconstitution of 10xHis-OsPIP1;3 in liposomes demonstra

149 Thus, reconstitution of a functional tissue must reestablish e

150 Here, we report the biosynthetic reconstitution of a lasso peptide derived from Thermobif

151 e used the same split inteins to control the reconstitution of a split Integrase-Recombination Direct

152 racting pairs of split inteins, which ensure reconstitution of active Cre when all fragments are expr

153 we present an optochemical tool which allows reconstitution of AJs by chemical dimerization of the fo

154 Here we present functional reconstitution of an MCU-EMRE complex from the red flour

155 nscription factor resulted in the successful reconstitution of antiviral defenses through the direct

156 Reconstitution of Apc(Min/+) or azoxymethane- and dextra

157 ed in vivo in CD68.hMcl-1 transgenic mice by reconstitution of apoptosis with BH3 mimetics or clodron

158 Here, we report on successful in vitro reconstitution of Arabidopsis (Arabidopsis thaliana) OHP

159 In contrast, genetic reconstitution of B(1)R exclusively in adipose tissue re

160 Reconstitution of BRCA1 resulted in significant decrease

161 alysis of membrane biophysics, and synthetic reconstitution of cellular machineries.

162 Reconstitution of CMV-specific T cell immunity was evide

163 ction in beta-cell-resident CPE, and genetic reconstitution of CPE in betaOGTKO islets rescued the dy

164 s multiphase organization using quantitative reconstitution of cytoplasmic stress granules (SGs) with

165 Reconstitution of DCNL1 monoubiquitylation in vitro reve

166 Reconstitution of effector, central and terminally diffe

167 Genetic reconstitution of eIF4E in single beta-cells or intact i

168 Notably, reconstitution of EMI1 expression reestablishes PARPi se

169 Here, we demonstrate the effective reconstitution of enzymatic tetracyclization of syntheti

170 Reconstitution of FAM46C in multiple myeloma cells that

171 is, genetic complementation, and biochemical reconstitution of FANCD2 monoubiquitination to determine

172 algorithm and achieved reliable cytoplasmic reconstitution of functional diphtheria toxin from engin

173 Here we report reconstitution of functional Saccharomyces cerevisiae ki

174 ultilineage engraftment of transduced cells, reconstitution of functional T cells and B cells, and no

175 ored, mechanical stimulation facilitates the reconstitution of functional tip links.

176 hepatic FXR-knockout and FXR-knockdown mice, reconstitution of FXR expression up-regulated cholestero

177 Microbial sequencing and reconstitution of germ-free mice have indicated both pos

178 The concentration and reconstitution of grape juice preserved the antioxidant

179 tion occurred between stem cell infusion and reconstitution of healthy immune cells.

180 IRE1alpha knockout leads to reduced reconstitution of HSCs.

181 ng identified accessory proteins that enable reconstitution of human alpha6beta4 nAChRs.

182 Here, we establish a protocol for in vitro reconstitution of human prospermatogonial specification

183 Reconstitution of IL-22 at late time points through retr

184 Reconstitution of immune responses on ART is associated

185 Reconstitution of individual cultivable gut bacteria in

186 trometry) for the automated and reproducible reconstitution of instant coffee products was developed

187 se of volatile organic compounds (VOCs) upon reconstitution of instant coffee products.

188 Consistent with this observation, reconstitution of IQGAP1-null cells with IQGAP1 GRD-2K s

189 econstitution, a new gentle approach for the reconstitution of labile membrane-protein complexes, and

190 Consistently, reconstitution of LC3B-KO cells with the phospho-mimicki

191 Reconstitution of lymphopenic hosts with Nod2(-/-) CD4(+

192 serum) and immediately after saline solution reconstitution of lyophilized serum (0), 15, and 30 days

193 Reconstitution of mast cells in W/W(V) mice restored the

194 Furthermore, reconstitution of MC-deficient mice with wild-type MCs,

195 Here, we compared the extraction and reconstitution of membrane proteins into lipid nanodiscs

196 Reconstitution of miR-3613-3p in resistant cells downreg

197 Inhibition of AURKA or reconstitution of miR-4715-3p inhibited GPX4 and induced

198 Here, in vitro reconstitution of MT dynamics with SEPT2/6/7, the minima

199 Here we describe reconstitution of MT nucleation using purified recombina

200 The reconstitution of Mycobacterium tuberculosis antigen-spe

201 h mutational analyses showed was crucial for reconstitution of necroptosis signaling.

202 Lastly, in vitro reconstitution of OlvS(A) activity showed that it is a l

203 Reconstitution of p110deltaD910A/D910A mice with p110del

204 Following reconstitution of PDE6 and activated Gt(alpha) with lipo

205 hat SVR with DAA therapy is effective in the reconstitution of phenotypic and functional abnormalitie

206 While the reconstitution of proteins from genetically encoded elem

207 This is accomplished by reconstitution of PTEN-null embryoid bodies with PTEN mu

208 Here, we utilize in vitro reconstitution of purified motor proteins and non-enzyma

209 aimed to determine whether dissociation and reconstitution of rat fetal testis tissue during the MPW

210 Finally, we show that reconstitution of RCC cells with a PRDM16 mutant unable

211 ue xenotransplants during the MPW results in reconstitution of seminiferous cords.

212 ed upon the knockout of SLC35B2, whereas the reconstitution of SLC35B2 in these cells fully restored

213 + ATG may limit GVHD occurrence, the quicker reconstitution of some NK cell subtypes may help with av

214 Here, we show that reconstitution of specific pre-mRNA cleavage with recomb

215 nd Sult1e1 knockout mice with liver-specific reconstitution of SULT1E1 expression to bilateral renal

216 Liver-specific transgenic reconstitution of SULT1E1 in Sult1e1 knockout mice aboli

217 s vesicles, represent key early steps in the reconstitution of synthetic cells.

218 Here, we report on the results of in vitro reconstitution of talin-vinculin-actin assemblies using

219 Reconstitution of TFF1 inhibits IL6-induced STAT3 transc

220 The reconstitution of TFF1 protein in human gastric cancer c

221 rane-bending ESCRT factors, are required for reconstitution of the active TBSV VRCs in GUVs, further

222 se to stress mediators as well as for serial reconstitution of the blood system in vivo.

223 Reconstitution of the coformycin biosynthetic pathway in

224 Thus, the incomplete reconstitution of the human immune system in BLT mice ma

225 Reconstitution of the LLO pathway to synthesize Man9GlcN

226 e we investigate how depletion and selective reconstitution of the maternal gut microbiome influences

227 Reconstitution of the microbiome with mature microbiota

228 in the citridone family through heterologous reconstitution of the pfp pathway.

229 GUV-based reconstitution of the TBSV replicase revealed the need f

230 Here, we present a complete reconstitution of these events.

231 ncAAs in human hematopoietic stem cells and reconstitution of this genetically engineered hematopoie

232 Reconstitution of this human cTnC variant into permeabil

233 We demonstrate that reconstitution of VRCs on GUVs with endoplasmic reticulu

234 Reconstitution of wild type (WT) AAMs to ST2-deficient m

235 -13 was sufficient for RSV-driven AHR, since reconstitution of wild-type ILC2 rescued RSV-driven AHR

236 ls had significant phenotypic and functional reconstitution post DAA therapy.

237 in the bone marrow and a marked reduction of reconstitution potential due to a blockage of HSC differ

238 old age, including myeloid skewing, reduced reconstitution potential, impaired self-renewal, and a p

239 ofiling could serve as a biomarker of immune reconstitution, predict clinical events after HCT, or he

240 d AA retention of nanoliposomes after powder reconstitution process by increasing the bilayer membran

241 The concentration and reconstitution process resulted in significant losses (T

242 The concentration and reconstitution processes of grape juices can result in l

243 t sorbent saturation, but the extraction and reconstitution protocol likely contributed to atrazine d

244 Here, we optimized a detergent-based reconstitution protocol to develop a proteoliposome tech

245 upon purification in cell-free biosynthetic reconstitution reactions.

246 Our biochemical reconstitutions recapitulate many observed in vivo prope

247 survival, overall survival (OS), and immune reconstitution.RESULTSNo participants showed evidence of

248 s, Catovirus and Moumouvirus) using in vitro reconstitution revealed that they possess eEF1A-like act

249 otein signalling (RGS) 16, and genetic RGS16 reconstitution reverses the effects of Arg2 overexpressi

250 Biochemical reconstitution shows that Bre1 binds the scaffold protei

251 outcomes are linked to the degree of immune reconstitution, specifically of T cells.

252 Genetic and biochemical reconstitution studies have revealed the enzymes and bin

253 F2-mutant sRCC model, YAP1 knockdown and NF2 reconstitution suppressed cell proliferation, tumour gro

254 in tuberculosis (TB) pathology and TB-immune reconstitution syndrome.

255 that beta(2)AR basal activity depends on the reconstitution system and further suggests that various

256 ndividual proteins from this simple in vitro reconstitution system leads to cable defects that closel

257 A minimal actin-myosin reconstitution system reveals that representatives of al

258 , we report the development of a biochemical reconstitution system that allowed us to investigate how

259 Here, we describe a yeast SG reconstitution system that faithfully recapitulates SG a

260 Here, we used a reconstitution system to discover that Cdk1, the major m

261 sue, Ruschhaupt et al (2019) used yeast as a reconstitution system to examine the functionality, plas

262 olecule FRET assays with a nanodisc membrane reconstitution system to investigate the conformational

263 Using an in vitro reconstitution system, we describe a mechanism for how m

264 In a reconstitution system, we find that the CNGC2 and CNGC4

265 Using a cell-free reconstitution system, we report that clathrin coat form

266 Using in vitro membrane reconstitution systems and cellular imaging approaches,

267 Using Xenopus egg extract and in vitro reconstitution systems, here we show that SSBs are first

268 Furthermore, we show through in vitro reconstitution that phosphorylation-mediated release of

269 thaliana Protein-protein interaction network reconstitution then revealed a highly interconnected and

270 New so-called immune reconstitution therapies (IRTs) have the potential to in

271 n needs to be considered in hepatocyte-based reconstitution therapy and possibly even in other settin

272 lity is crucial to cell survival and protein reconstitution, these results highlight the importance o

273 owed by gradual T cell and more rapid B cell reconstitution to a repertoire deviated toward naive cel

274 and detergent environments, using functional reconstitution to define lipids appropriate for structur

275 transcriptomics, metabolic logic and pathway reconstitution to elucidate a near-complete biosynthetic

276 mice were significantly improved after fecal reconstitution, transplantation of monocytes, or dietary

277 Fecal reconstitution, transplantation of monocytes, or dietary

278 The NK cell repertoire reconstitution was examined by multiparameter flow cytom

279 a consequence, the hematopoietic compartment reconstitution was improved because of the recovery of t

280 Immune reconstitution was monitored through flow cytometry and

281 T-cell immune reconstitution was robust among CB transplants, although

282 Although thymocyte reconstitution was transient, gene-corrected peripheral

283 Using anaerobic in vitro reconstitution, we establish that a set of six enzymes i

284 Xenopus laevis egg extracts and biochemical reconstitution, we found that autoinhibition of the Mis1

285 Using Xenopus egg extracts and biochemical reconstitution, we found that Sgo1 interacts directly wi

286 Employing in vitro reconstitution, we show that the LIM domain-containing r

287 To elucidate mechanisms involved in immune reconstitution, we studied immune activation, immune exh

288 t myeloid-biased differentiation and reduced reconstitution while NEO1(-) Hoxb5 (+) LT-HSCs are linea

289 Reconstitution with a single bacterial species, Clostrid

290 betic mice, macrophage depletion followed by reconstitution with BMMO-derived HuR-deficient exosomes

291 hage ablation retarded tumor growth, whereas reconstitution with galectin-3-positive bone marrow rest

292 employ both optical trapping and biochemical reconstitution with myosin motor proteins to show single

293 Indeed, although a slower T cell reconstitution with PTCY + ATG may limit GVHD occurrence

294 Using biochemical reconstitution with purified proteins, we demonstrate th

295 d composition, 2) detergent-mediated protein reconstitution with subsequent detergent removal, 3) gen

296 of human PORCN and confirm, through in vitro reconstitution with the purified enzyme, that PORCN is n

297 Reconstitution with wild type TRPM2 or Nrf2, but not TRP

298 Here, using in vitro reconstitutions with purified components, we show that K

299 rrays in nanopore sensing devices, where the reconstitution, with high spatiotemporal precision, of s

300 -induced thymic damage to the delayed T cell reconstitution, witnessed after rATG treatment.