ライフサイエンスコーパス: recoverin

1 , F83, and Y86) resembling that of Ca2+-free recoverin.

2 sented based on the NMR data and homology to recoverin.

3 n, unlike the myristoyl group in Ca(2+)-free recoverin.

4 one bipolars immunoreactive for calbindin or recoverin.

5 ity in Ca(2+) binding, as seen previously in recoverin.

6 overall structure of Frq1 resembles that of recoverin.

7 ct tandem array like that seen previously in recoverin.

8 ring the Ca2+-bound structures of GCAP-2 and recoverin.

9 bipolar cells were labeled with antiserum to recoverin.

10 ylated recoverin to 400 nM for myristoylated recoverin.

11 by Ca2+ and Ca2+-binding proteins, including recoverin.

12 y for phosphodiesterase-gamma, arrestin, and recoverin.

13 myristoyl switch similar to the one found in recoverin.

14 to enter and cause death of cells expressing recoverin.

15 teraction underlies the inhibitory effect of recoverin.

16 fic EF hand Ca2+-binding proteins defined by recoverin.

17 calbindin bipolar cells are also labeled for recoverin.

18 potency of its effect is similar to that of recoverin.

19 The only retinal antibody not present was recoverin.

20 ent inhibition of rhodopsin kinase (GRK1) by recoverin.

21 expressing various levels of GRK1 or lacking recoverin.

22 y for binding of Ca(2+) to non-myristoylated recoverin.

23 nd light is nearly eliminated by deletion of recoverin.

24 T cell immunity toward the retinal protein, recoverin.

25 ness, and bipolar cell types using Chx10 and recoverin.

26 s a sequestered myristoyl group like that of recoverin.

27 its association with T cell responses toward recoverin.

28 immunolabeling with PCNA and either F4/80 or recoverin.

29 od differentiation: rod opsin, arrestin, and recoverin.

30 odopsin (Rh*) by Ca(2+)-dependent binding of recoverin, (2) guanylyl cyclase activity via Ca(2+)-depe

31 but prevent the loss of proteins, including recoverin (25 kDa).

32 Recoverin, a 23-kDa Ca(2+)-binding protein of the neuron

33 One such protein is recoverin, a calcium sensor in retinal rod cells, which

34 An antibody to recoverin, a calcium-binding protein found in photorecep

35 Recoverin, a member of the EF-hand protein superfamily,

36 Recoverin, a member of the EF-hand superfamily, serves a

37 Recoverin, a member of the neuronal calcium sensor branc

38 tirecoverin antibodies were purified using a recoverin-affinity column.

39 An immunodominant peptide of recoverin, AG-16, was capable of inducing disease in IL-

40 Interestingly, while the recoverin and calmodulin-binding sites in GRK1 do not ov

41 e highly sequestered myristoyl group seen in recoverin and GCAP1.

42 e feedback on cone phototransduction through recoverin and GRK1 are not well understood.

43 Rods and cones share the same isoforms of recoverin and GRK1, and photoactivation also triggers a

44 In addition, two of the proteins, recoverin and guanylate cyclase activating protein (GCAP

45 , focusing on three Ca(2+)-binding proteins, recoverin and guanylate cyclase activating proteins 1 (G

46 Given the similarities between recoverin and neurocalcin, we examined the effect of Dro

47 genic mutant mice to unravel the function of recoverin and phosducin and to further define the role o

48 Based on their staining with recoverin and salient morphological features, these ecto

49 tibodies against the calcium-binding protein recoverin and the carbohydrate epitope 15 (CD15) as reli

50 Previous experiments genetically deleted recoverin and the GCAPs and showed that significant regu

51 plexiform layer and in immunoreactivity for recoverin and/or CD15.

52 he nerve fiber layer are positive for TULP1, recoverin, and blue cone opsin.

53 inear array, similar to that seen in KChIP1, recoverin, and other structures of the neuronal calcium

54 ns, namely suppression of tumorigenicity 13, recoverin, and Ppib and the fourth binds to human Lactof

55 toreceptor cells, as identified by vimentin, recoverin, and rhodopsin immunocytochemical staining.

56 yclase activating protein, protein kinase A, recoverin, and transducin) are N-terminally modified wit

57 ng carcinoma: retinopathy ("CAR"-IgG [23kDa, recoverin]) and optic neuritis collapsin response-mediat

58 e ratio of rhodopsin kinase to its modulator recoverin appears critical for the proper adaptation of

59 Recoverin appears in cones across 70% of the retina at f

60 e studies showed that antibodies specific to recoverin are able to enter and cause death of cells exp

61 d on regulators of shut-off kinetics such as Recoverins, Arrestins, Opsin kinases, and Regulator of G

62 The first eight residues of recoverin at the N terminus are solvent-exposed, enablin

63 of (15)N-labeled Ca(2+)-bound myristoylated recoverin bind anisotropically to phospholipid membranes

64 eport here the NMR structure of Ca(2+)-bound recoverin bound to a functional N-terminal fragment of r

65 Calsenilin/DREAM/KChIP3, a member of the recoverin branch of the EF-hand superfamily, interacts w

66 At high Ca2+ (Ca), myristoylated recoverin (Ca-recoverin) prolonged the recovery phase of

67 )H NMR order parameter analysis performed on recoverin containing a fully deuterated myristoyl group,

68 nglion cells were shortened when measured in recoverin-deficient retinas.

69 EF-1, as in recoverin, does not bind calcium because it contains a d

70 Instead, it is an effect of recoverin downstream of phototransduction in rods that p

71 , autoantibodies originally elicited against recoverin expressed in tumor cells may damage retinal ph

72 oup covalently attached to the N-terminus of recoverin facilitates its binding to retinal disk membra

73 oup covalently attached to the N-terminus of recoverin facilitates the binding of recoverin to retina

74 f GCAP-2, like those of other members of the recoverin family of Ca2+-binding proteins, is fatty acyl

75 calcium-dependent manner, reminiscent of the recoverin family of calcium-myristoyl switch proteins.

76 m-binding motif) that is most similar to the recoverin family of myristoyl switch proteins.

77 main that is related to the N termini of the recoverin family of neuronal calcium sensors.

78 Calsenilin is a member of the recoverin family of neuronal calcium-binding proteins th

79 labeled recoverin to membranes and show that recoverin favors membranes with negative curvature and h

80 ylation had little effect on the affinity of recoverin for the kinase, but it raised the K0.5 for Ca2

81 In the present work, the affinity of recoverin for the negatively charged phosphatidylserine

82 Frq1 belongs to the recoverin/frequenin branch of the EF-hand superfamily an

83 of four Ca(2+)-sensor proteins (calmodulin, recoverin, GCAP1, and GCAP2) operating in the vertebrate

84 hors found no evidence that mutations in the recoverin gene are a cause of RP or another of the hered

85 n phenotype associated with mutations of the recoverin gene remains unknown.

86 odulin-binding sites in GRK1 do not overlap, recoverin-GRK1 interaction is inhibited by calmodulin, m

87 unique N-terminally localized GRK1 site for recoverin had no clearly defined structural characterist

88 Recoverin has a relative molecular mass of 23,000 (M[r]

89 -/-) rods, in which the GRK1-binding protein recoverin has been genetically deleted.

90 Recoverin has been identified as a target autoantigen fo

91 Recoverin has been postulated to inhibit the kinase in d

92 Recoverin has two functional EF hands and a myristoylate

93 One such protein, recoverin, has been proposed to regulate RK activity con

94 he myristoyl binding site and two swivels in recoverin homologues from yeast to humans indicates that

95 It also contains an N-terminal recoverin homology (RVH) domain that is related to the N

96 ytes into the eyes dramatically increased in recoverin-immunized IL-10 KO mice.

97 cell (protein kinase C-alpha [PKC-alpha] and recoverin) immunofluorescence revealed the maintenance o

98 it may reduce the inhibitory constraint that recoverin imposes on rhodopsin kinase, an enzyme respons

99 icular lipids and of specific amino acids of recoverin in its membrane binding has not yet been demon

100 serine, whereas the extent of penetration of recoverin in phosphatidylserine monolayers was estimated

101 that light causes a significant reduction of recoverin in rod outer segments, accompanied by its redi

102 GCAP-2 differs from recoverin in that the calcium ion binds to EF-4 in addit

103 lex is similar to structures of Ca(2+)-bound recoverin in the absence of target (<1.8A root-mean-squa

104 The overall main-chain structure of recoverin in the complex is similar to structures of Ca(

105 origin and identity of the cells expressing recoverin in the ganglion cell layer of the rat retina.

106 ed for the membrane binding of myristoylated recoverin in the presence of calcium.

107 oantibody to recoverin, when given access to recoverin in the retina through the blood-retina barrier

108 periments indicate that rhodopsin kinase and recoverin, in addition to their well-known role in regul

109 The myristoyl (C14:0) modification of recoverin increased its activity 12-fold, and the C12:0

110 gy model of CIB based upon calcineurin B and recoverin indicated a conserved hydrophobic pocket withi

111 It has been shown that recoverin inhibits the phosphorylation of rhodopsin when

112 Recoverin is a 23 kDa myristoylated Ca2+-binding protein

113 Recoverin is a heterogeneously acylated calcium-binding

114 Recoverin is a protein in rods that prolongs phototransd

115 Recoverin is a small calcium binding protein involved in

116 results demonstrate that membrane binding by recoverin is achieved primarily by insertion of the myri

117 We propose that Ca(2+)-bound recoverin is bound between rhodopsin and RK in a ternary

118 studies revealed that the myristoyl group of recoverin is sequestered inside the protein core in the

119 The Ca(2+)-binding protein recoverin is thought to regulate rhodopsin kinase and to

120 this no longer occurs in mice that have had recoverin knocked out.

121 The binding of two Ca2+ ions to recoverin leads to its translocation from the cytosol to

122 second and third EF-hands (EF-2 and EF-3) of recoverin leads to the extrusion of the fatty acid.

123 l cyclase-activating protein 2 (GCAP-2) is a recoverin-like calcium-binding protein that regulates ph

124 esponding fragments of other closely related recoverin-like proteins that do not regulate RetGC.

125 s to be an assayable property of a subset of recoverin-like proteins.

126 exposed hydrophobic groove on the surface of recoverin lined by side-chain atoms of Trp-31, Phe-35, P

127 tivation and that the Ca(2+)-binding protein recoverin may be required for the light-dependent modula

128 These experiments support the notion that recoverin mediates Ca-dependent inhibition of rhodopsin

129 d calcium-binding studies of a myristoylated recoverin mutant (myr-E85Q) designed to abolish high-aff

130 calcium-binding proteins closely related to recoverin, neurocalcin, and many other neuronal Ca(2+)-s

131 neuronal calcium sensor (NCS) proteins (e.g. recoverin, neurocalcins, and frequenin) are expressed at

132 sor protein-1 (NCS-1), another member of the recoverin-neuronal calcium sensor superfamily, is expres

133 Unexpectedly, the effect of recoverin on postsynaptic signals could not be explained

134 Transplanted cells coexpressed GFP and recoverin only in the ONL.

135 ng N-terminal region can be substituted with recoverin or neurocalcin sequences without loss of GCAP-

136 corresponding fragments from neurocalcin or recoverin, or even partially deleted without preventing

137 Dye injections of recoverin- or CD15-prelabeled cone bipolar cells in vert

138 photoreceptor-specific Ca2+-binding protein recoverin, other GRKs can be inhibited by Ca2+-calmoduli

139 inal mixed cultures, MCP-1 was cytotoxic for recoverin+ photoreceptors, and this toxicity was elimina

140 The N-terminal region of recoverin points toward the membrane surface, with close

141 strate that early in development some of the recoverin-positive cells in the ganglion cell layer are

142 velopment, there were also a small number of recoverin-positive cells of unknown origin in the gangli

143 Recoverin-positive cells were apparent in the ventricula

144 These recoverin-positive cells were not double-labeled by cell

145 Another contingent of recoverin-positive cells, with morphological features eq

146 ologies of photoreceptors, horizontal cells, recoverin-positive OFF-cone bipolar cells, rod bipolar c

147 cells is not required for the segregation of recoverin-positive On and Off cone bipolar cell projecti

148 d biotinylated antibodies were cultured with recoverin-positive rat retinal cells E1A.NR3.

149 the calcium-binding proteins calmodulin and recoverin, posttranslational isoprenylation and palmitoy

150 We also find that recoverin potentiates the sensitivity of cones in dim li

151 high Ca2+ (Ca), myristoylated recoverin (Ca-recoverin) prolonged the recovery phase of the bright fl

152 pigment epithelium-derived factor (PEDF) and recoverin (RCV1) map to this region and are candidate ge

153 These cells expressed photoreceptor proteins recoverin, red opsin, and rhodopsin, and displayed morph

154 Immunization of C57BL/6 mice with recoverin resulted in ocular inflammation including infi

155 Indeed, calcium binding by recoverin results in the extrusion of its myristoyl grou

156 ra of recoverin suggests that membrane-bound recoverin retains the same overall three-dimensional str

157 luding presumptive photoreceptors expressing recoverin, rhodopsin, or cone opsin.

158 ported effects of adenine nucleotides on the recoverin-rhodopsin kinase binding.

159 ristoylation, and adenine nucleotides on the recoverin-rhodopsin kinase interaction.

160 in when Ca2+ is present and that a dependent recoverin/rhodopsin kinase interaction underlies the inh

161 -B1/B2, GAD67, GLRA1b, GRM1, GRM5, Hu, LGl1, recoverin, Ri, ZIC4).

162 The recoverin-RK25 complex exhibits Ca(2+)-induced binding t

163 Current retinal antibody testing, other than recoverin, should be interpreted with caution, especiall

164 a of uniformly and selectively (15)N-labeled recoverin show that the Ca(2+)-bound protein is position

165 observed throughout the culture treated with recoverin specific antibodies but not with normal antibo

166 This model supports the hypothesis that recoverin-specific T cell responses are major drivers of

167 Adoptive transfer of recoverin-stimulated cells into naive mice was sufficien

168 al myristoylation, similar to members of the recoverin subfamily and are fatty acid acylated in vitro

169 em array similar to GCAP2 and members of the recoverin subfamily of Ca2+-binding proteins.

170 t GCIP is a Ca2+-binding protein of the GCAP/recoverin subfamily.

171 1 were very similar to those of Ca(2+)-bound recoverin, suggesting that the overall structure of Frq1

172 nce of ncs1Delta was not affected by cAMP or recoverin, suggesting that the two ncs1Delta phenotypes

173 al and calculated solid-state NMR spectra of recoverin suggests that membrane-bound recoverin retains

174 osed drop in Ca concentration, the effect of recoverin switched off with little lag.

175 Residues of recoverin that contact RK25 are highly conserved, sugges

176 ls were visualized using an antibody against recoverin, the calcium binding protein that labels On an

177 ls were visualized using an antibody against recoverin, the calcium-binding protein that labels the s

178 An antibody against recoverin, the calcium-binding protein, labels photorece

179 inding proteins such as calmodulin (CaM) and recoverin, the molecular mechanisms are poorly understoo

180 he K0.5 for Ca2+ from 150 nM for nonacylated recoverin to 400 nM for myristoylated recoverin.

181 analyses confirmed that Ca2+ is required for recoverin to bind RK.

182 e Ca(2+)-responsive translocation of labeled recoverin to membranes and show that recoverin favors me

183 ion of the specific binding of myristoylated recoverin to phosphatidylserine, whereas the extent of p

184 inus of recoverin facilitates the binding of recoverin to retinal disk membranes by a mechanism known

185 We suggest that recoverin translocation is adaptive because it may reduc

186 Recoverin undergoes a calcium-myristoyl switch during vi

187 In both cases the majority of recoverin was found in rod inner segments, with approxim

188 Moreover, the calcium-myristoyl switch of recoverin was only observed upon binding onto monolayers

189 Results suggest that autoantibody to recoverin, when given access to recoverin in the retina

190 sidues 2-90) is similar to that of Ca2+-free recoverin, whereas the C-terminal region (residues 100-2

191 f ncs1Delta was also complemented by retinal recoverin, which controls Ca2+-regulated desensitization

192 cule or protein the myristoyl-switch protein recoverin, which is involved in rhodopsin-mediated signa

193 epresents a hybrid between the structures of recoverin with zero and two Ca2+ bound.

194 Cells not expressing recoverin (Y79, PC12, and GH3) were not susceptible to c