ライフサイエンスコーパス: refolding

1 d very high energy barriers of unfolding and refolding).

2 rin cytoskeleton, including domain unfolding/refolding.

3 onformational changes that trigger F protein refolding.

4 compact and extended states populated during refolding.

5 e of events could elucidate the mechanism of refolding.

6 , and produce 41.5 zJ mechanical work during refolding.

7 ffinity purification and solid-phase protein refolding.

8 s that H223 protonation guards against early refolding.

9 protein aggregates, leading to their native refolding.

10 repeated rounds of stepwise G4-unfolding and refolding.

11 it C-terminus additionally assist in subunit refolding.

12 cient time for protein chaperones to attempt refolding.

13 agglutinin started to undergo conformational refolding.

14 er, enzymatic reactions, and protein un- and refolding.

15 1, to partner with Hsp70 in in vitro protein refolding.

16 transferred to ATP-dependent chaperones for refolding.

17 d using limited proteolysis and denaturation/refolding.

18 gy transfer in the microsecond time range of refolding.

19 refolding, and, subsequently, again tertiary refolding.

20 everal times faster than that of spontaneous refolding.

21 ve regions of a protein chain and preventing refolding.

22 e mechanism of chaperone-assisted Luciferase refolding.

23 ve helical structure frustrating microsecond refolding.

24 te structural dynamics with global unfolding/refolding.

25 ammaD-crystallin aggregation suppression and refolding.

26 trap as the reason for slower P-jump-induced refolding.

27 vitro assay for this step in fusion protein refolding.

28 an anticipated from models involving protein refolding.

29 from one another, leading to native protein refolding.

30 olded protein but did not affect the rate of refolding.

31 and allowed a folding pathway different from refolding.

32 and ligand binding-dependent conformational refolding.

33 s to undergo ligand-dependent conformational refolding.

34 d) and the P3-strengthening mutations slowed refolding (6- to 1400-fold), suggesting that P3 indeed u

35 one mutation abolished both Hsc70 ATPase and refolding activities.

36 the analyzed mutants show altered ATPase and refolding activity caused by changes in Hsp40 binding.

37 a similar structure, ATP use, and substrate refolding activity, and, importantly, it also inhibits m

38 less than temperature denaturation, protein refolding after a fast P-jump is not necessarily faster

39 Upon refolding after chemical denaturation, this protein prod

40 vinculin and vinculin binding inhibits talin refolding after force is released.

41 e of properly folded rRhi o 2 showed partial refolding after heat denaturation.

42 unfolding temperature but rather facilitated refolding after thermal stress.

43 We proposed a possible "refolding-after-unfolding" mechanism, as further support

44 However, "refolding-after-unfolding" with increasing in-source act

45 However, in lieu of fostering their refolding, Aha1 allows ubiquitination of bound clients b

46 nins up to 80 degrees C, followed by partial refolding and aggregation at even higher temperatures.

47 mass spectral profiles, which reveal gradual refolding and concomitant deprotonation of higher charge

48 ich, marginally stable oligomers in vitro on refolding and cross-beta-rich aggregates following incub

49 space and integrated with the cell's protein refolding and degradation pathways remains unclear.

50 Allosteric effectors that mediate refolding and enhance enzymatic function have the potent

51 RiC and were active as assayed by luciferase refolding and human gammaD-crystallin aggregation suppre

52 apable of growing on the ends by seeding the refolding and incorporation of the normal form of the gi

53 s corresponding to HR1 or HR2 interrupt gp41 refolding and inhibit HIV infection.

54 Both experimental refolding and Monte Carlo simulations of Markov state mo

55 ssary early step for paramyxovirus F-protein refolding and presents a novel target for structure-base

56 ess conditions, when both chaperone-mediated refolding and proteasomal degradation are compromised or

57 on in Escherichia coli followed by oxidative refolding and proteolysis.

58 nduced G-quadruplex formation and subsequent refolding and provides key insight into rate-limiting st

59 activated beta-cardiac myosin is followed by refolding and reactivation of ATPase and motile activiti

60 entropic spring effects can lead to polymer refolding and reformation of the previously cleaved meta

61 is, including quality control during protein refolding and regulation of protein degradation.

62 rol pathways, preferentially Hsp70-dependent refolding and selective autophagy.

63 hibited complex dynamics, including frequent refolding and state occupancies of <10 mus.

64 l-atom simulations of pressure drops capture refolding and unfolding of all three mutants by a simila

65 tigens, this antigen preparation induces MR1 refolding and upregulates surface expression of human MR

66 prepared by cell-free expression, functional refolding, and reconstitution into lipid membranes.

67 h display tertiary unfolding, then secondary refolding, and, subsequently, again tertiary refolding.

68 arance pathways involved in the recognition, refolding, and/or clearance of aberrant proteins.

69 ed using typical dialysis- or dilution-based refolding approaches.

70 ionally folding protein whose extremely slow refolding (approximately days) is catalyzed by chaperone

71 ecrease in the rates of enzyme unfolding and refolding as well as a reduction of the intrinsic fluctu

72 Refolding assays demonstrate obligate single- and double

73 In vitro refolding assays under redox conditions showed that POFU

74 of the combinations in ATPase and luciferase refolding assays were dependent on the identity and stoi

75 Here, using in vitro phosphorylation and refolding assays, analytical size-exclusion chromatograp

76 ith fluorescence polarization and luciferase-refolding assays, we report the unexpected discovery of

77 phase, but also of a ~1.5 ms "slow" phase of refolding, attributed to non-native helical structure fr

78 Denaturation and refolding behavior of the allergen confirmed that its Ig

79 cium, the R1597W mutation slowed the rate of refolding but had no effect on unfolding.

80 controlled aggregation and facilitates their refolding by ATP-dependent Hsp70-Hsp100 disaggregases.

81 The high thermal stability, refolding capacity, and resistance to gastrointestinal e

82 amino acid foldon at the C terminus and slow refolding channeled gp41 into trimers.

83 olution description of unfolded states under refolding conditions for the N-terminal domain of the L9

84 renders automated buffer exchange to screen refolding conditions impossible.

85 by refolding is challenging because suitable refolding conditions must be empirically determined for

86 based determinations with respect to varying refolding conditions.

87 nd fold rapidly with overlapping melting and refolding curves, G3T multimers (G3T units covalently at

88 We present unfolding and refolding data for the small, single-domain protein ddFL

89 hypothesized that much like disaggregation, refolding, degradation, and even normal function, Hsc70

90 e resulting method, which we deem DSF guided refolding (DGR), thus enables the production of aggregat

91 0-gp41 functional interactions affecting Env refolding during HIV entry.

92 olecule, a region that undergoes substantial refolding during host-cell entry.

93 cessible means to characterize the unfolding/refolding dynamics of individual molecules and resolve c

94 ifferent kinetic constants for unfolding and refolding even though the process remains experimentally

95 (H or G) triggers F to undergo an extensive refolding event to form a stable postfusion state.

96 insights into the mechanics of this critical refolding event.

97 Using real-time refolding experiments monitored by CD and NMR, we show t

98 Recent P-jump refolding experiments on the helix bundle lambda-repress

99 Single-molecule refolding experiments reveal the initial nucleation of f

100 ptical sensing modules with consideration of refolding feature of aptamers, selection of anchoring ph

101 DX is mediated by opening/closing (unfolding/refolding) fluctuations.

102 In this study, we examine the mechanism of refolding for two distinct rhomboids to gain insight int

103 s at low forces enabled the determination of refolding forces of about 2 pN.

104 ces the difference between the unfolding and refolding forces, bringing the non-equilibrium unfolding

105 oprotein obtained following denaturation and refolding forms a hexamer.

106 n-prone and disulfide-containing proteins by refolding from E. coli inclusion bodies, which would not

107 As F undergoes a dramatic refolding from its prefusion to postfusion conformation,

108 The P3-weakening mutations accelerated refolding from M (3- to 30-fold) and the P3-strengthenin

109 e P3-weakening mutations were larger than in refolding from M, and small-angle X-ray scattering showe

110 olding of native peripheral structure during refolding from M, which probably permits rearrangement o

111 cid-induced fusion, as well as insights into refolding from pre- to post-fusion conformations.

112 e helix-turn interfaces that should speed up refolding from the pressure-denatured state, if this hyp

113 volves repetitive cycles of G4 unfolding and refolding fueled by ATP hydrolysis.

114 thus far, however, have largely focussed on refolding full-length proteins from artificially induced

115 e that FtsH contains the protease as well as refolding functions, and both the AAA and the proteolyti

116 nfolding of G4-RNA followed by ATP-dependent refolding, generating a highly asymmetric pattern of act

117 Thus, pressure-jump (P-jump)-induced protein refolding, if it could be made fast enough, would be ide

118 a-repressor mutant is nonetheless capable of refolding in a single explicit solvent MD trajectory in

119 g the non-two-state behavior observed during refolding in molecular dynamics simulations.

120 as measured by the inhibition of luciferase refolding in prostate cancer cells.

121 nhibitory effect of TOMM34 on HSP70-mediated refolding in vitro In contrast, we noted that TOMM34 in

122 ev1) disrupts G4 DNA structures and prevents refolding in vitro.

123 ve the kinetics of spontaneous unfolding and refolding in zero urea.

124 Here we report a rapid method for refolding inclusion-body-based, recombinant cell surface

125 We probed the formation of a refolding intermediate by time-resolved fluorescence ene

126 ibrium unfolding intermediate and a distinct refolding intermediate from kinetics studies.

127 nant virions efficiently and were capable of refolding into a postfusion conformation without tempora

128 rting into the target cell membrane and then refolding into a postfusion structure that fuses the vir

129 rting into the target cell membrane and then refolding into a postfusion structure that fuses the vir

130 rting into the target cell membrane and then refolding into a postfusion structure that fuses the vir

131 usogenic glycoproteins of these pathogens is refolding into a thermodynamically highly stable fusion

132 he unfolded protein prevents rubredoxin from refolding into its native holo state.

133 ediate structures of the MACPF domain during refolding into the beta-barrel pore establish a structur

134 mers from the aggregate and their subsequent refolding into the native conformation.

135 In practice, however, protein production by refolding is challenging because suitable refolding cond

136 Loop refolding is limited by the hydrophobic collapse of the

137 ied the antibody from Escherichia coli after refolding it from inclusion bodies.

138 NTE as influencing the chaperone-independent refolding kinetics and overall thermodynamic stability o

139 we report the force-dependent unfolding and refolding kinetics of all talin rod domains.

140 of microsecond pressure and temperature jump refolding kinetics of the engineered WW domain FiP35, a

141 cond pressure-jump apparatus, we monitor the refolding kinetics of the helix-stabilized five-helix bu

142 We compared the NMR-derived refolding kinetics with data derived from thermal hyster

143 of folding against force and accelerates the refolding kinetics.

144 asured the force dependence of unfolding and refolding kinetics.

145 n-random client proteins of the Hsp104/Hsp70-refolding machinery, including the prion Sup35.

146 es involved in normal protein maturation and refolding malformed proteins through the unfolded protei

147 partners and that context-dependent protein refolding may be widespread in nature.

148 Because MSD refolding may turn off GPIb-IX's mechanosensory signals,

149 ocyanin secondary structure but alters their refolding mechanism and dodecameric structure.

150 ts demonstrate a cooperative, self-chaperone refolding mechanism, whereby the beta-subunits independe

151 Thus, activation or restoration of refolding mechanisms may alleviate TDP-43 aggregation in

152 BiP's ATPase activity, which is required for refolding misfolded proteins while coping with ER stress

153 oduces a kinetic barrier that partitions the refolding molecules to the nonpermuted structure.

154 These experiments reveal that refolding monitored by 1-3 contact formation indeed is m

155 s tested, arginine was the most effective in refolding mutant of pVHL.

156 We observe that the refolding nature of the aptamer and its combination with

157 rs in one discrete step at forces >10 pN and refolding occurred at lower forces showing hysteresis.

158 our results suggest that initial Luciferase refolding occurs along a vectorial pathway and also sugg

159 we measure the amount of ATP used for native refolding of a misfolded group I intron ribozyme by CYT-

160 s of the temperature dependent unfolding and refolding of a titin immunoglobulin domain and alpha-act

161 nometre-scale rearrangement is controlled by refolding of an evolutionarily conserved 'tongue', which

162 promotes HeLa cell survival and enhances the refolding of an Hsp90 substrate inside the cell.

163 The structures also reveal refolding of an S1 subdomain after ACE2 binding that dis

164 f the SDS from the protein-SDS complexes and refolding of betaLG, BSA, and lysozyme, while alphaLA ch

165 Refolding of both proteins results in reassociation of t

166 er-domain packing through a short linker and refolding of CD2.

167 f HMLalpha as the donor, when we perturb the refolding of chromosome III.

168 helix lead to increased stability and faster refolding of collagen peptides containing aza-glycine.

169 of Hsp90 interacts with disordered CTA1, and refolding of CTA1 by Hsp90 is dependent upon ATP hydroly

170 es a process that couples the Hsp90-mediated refolding of CTA1 with CTA1 extraction from the ER.

171 haperones Ydj1 and Sis1, and do not catalyze refolding of denatured proteins in vitro in cooperation

172 mc and also inhibits Cosmc-assisted in vitro refolding of denatured T-synthase.

173 Refolding of disordered CTA1 occurred in the presence of

174 Yet ARF6 alone did not promote the refolding of disordered CTA1 to an active state.

175 hundred ATP molecules are hydrolyzed during refolding of each ribozyme molecule.

176 tained from stopped-flow measurements of the refolding of Escherichia coli adenylate kinase were anal

177 other DNA polymerases, or hRev1 can prevent refolding of G4 DNA structures.

178 the activation energy required to cause the refolding of gB from a prefusion to a postfusion conform

179 ion pattern of gB, possibly representing the refolding of gB from its prefusion to its postfusion con

180 virus (EBV) is thought to be mediated by the refolding of glycoprotein B (gB) from a prefusion to a p

181 ical energy through mechanical unfolding and refolding of isopeptide bond-delimited polypeptide loops

182 ypeptides and mediate the out-of-cage native refolding of large proteins.

183 ging to observe the reversible unfolding and refolding of metalloproteins because of a loss or decomp

184 to chaperones, which aid in the clearance or refolding of misfolded proteins.

185 e native folding of nascent polypeptides and refolding of misfolded species, thereby buffering mutati

186 for bacterial expression, purification, and refolding of murine p28.

187 At slow pulling speeds (<50 nm s(-1)), the refolding of NuG2 can be clearly observed.

188 In addition, helix-structural refolding of PDGF binding aptamers (PBA) indirectly wrap

189 sms controlling the mechanical unfolding and refolding of proteins cannot be accessed by protein fold

190 biquitous chaperone is to participate in the refolding of proteins denatured by cytoplasmic stress, t

191 talytic enzyme reaction is used to stimulate refolding of proteins during real-time analysis.

192 l as prolamin precursor proteins involved in refolding of proteins.

193 pid cofactors may facilitate the spontaneous refolding of PrP into an infectious form while also allo

194 rences between protein folding in the ER and refolding of purified proteins.

195 pe III secretion, although contribution from refolding of secreted proteins has not been ruled out.

196 ozymes could be restored by putative t2M/t4M refolding of stem secondary structure or tertiary bridgi

197 hat force-dependent stochastic unfolding and refolding of talin rod domains make talin a very effecti

198 Surprisingly, we find that during refolding of tandem repeats, independent of sequence ide

199 Defective refolding of TDP-43 is predicted to aggravate the TDP-43

200 rotein and RNA components requires extensive refolding of the 16S rRNA and is assisted by 10-20 assem

201 In vitro, OsmY assisted in the refolding of the antigen 43 beta-barrel domain and prote

202 -n-butylammonium chloride results in dynamic refolding of the catalyst from the native fold to the an

203 ring, which enables reversible unfolding and refolding of the chains.

204 tute for DnaJ2 or GrpE in the DnaK2-assisted refolding of the denatured substrates.

205 Upon relaxation, refolding of the ferredoxin-like domains enables the hyd

206 chemokine receptors on target cells triggers refolding of the gp41 transmembrane subunit into a six-h

207 diated GQ unfolding is typically followed by refolding of the GQ, a pattern that is repeated several

208 dependent conformational destabilization and refolding of the hole-hole homodimer Fc.

209 Here, the reversible unfolding-refolding of the iron-sulfur protein rubredoxin was obse

210 terized the molecular events associated with refolding of the metastable prefusion S glycoprotein to

211 Functional rescue was observed on refolding of the oligo-T/U strands into bPNA triplex hyb

212 er virus, we show that these changes involve refolding of the protein into a trimeric state.

213 and peptides suggests that SER5 also delays refolding of the remaining fusion-competent Env trimers.

214 This bridging action requires a complete refolding of the RfaH C-terminal domain (CTD) from an al

215 observed in the p53-MDM2 complex and induces refolding of the short, unstructured MDM2 N-terminal reg

216 otif affects only late intermediate R3, when refolding of the tongue and docking to the GAF1 domain a

217 Refolding of the U-loops into bPNA triplex stems complet

218 ified based on changes in the elasticity and refolding of the unfolded polyprotein in the presence of

219 Here, we study pressure-drop refolding of three lambda-repressor fragment (lambda(6-8

220 pendent activation, titin elastic recoil and refolding of titin domains as an energy source, and Ca(2

221 output: conformational changes that trigger refolding of trimeric fusion proteins and membrane fusio

222 e MACPF domain, accompanied by extrusion and refolding of two alpha-helical regions into transmembran

223 s suggest a possible mechanism for promoting refolding of Type III effectors after delivery into host

224 DnaK2 chaperone system, HtpG enhanced native refolding of urea-denatured lactate dehydrogenase and he

225 These results establish refolding of yeast chromosome III as a key driving force

226 ressure-drop relaxation experiments captured refolding on a millisecond time scale.

227 finity interaction leading to conformational refolding on a ~1-s timescale at 36 degrees C.

228 endent manner, and supports their productive refolding once nonstress conditions are restored.

229 site and may inhibit fusion by preventing F refolding or by blocking the F-HN interaction.

230 to dissociate aggregates and thereby enables refolding or degradation of misfolded proteins.

231 by unfolding aberrant and toxic proteins for refolding or proteolytic degradation.

232 e, or does the extraction process facilitate refolding (or unfolding)?

233 ng temperature and was footprinted along the refolding pathway using fast photochemical oxidation of

234 key conformational changes in the F-protein refolding pathway, but a detailed understanding of prefu

235 folding intermediates during its spontaneous refolding pathway.

236 nd active (beta-barrel) states and plausible refolding pathways have been reported, how this reversib

237 this modelling indicates whether alternative refolding pathways might occur upon cooling.

238 However, aggregation during the refolding process is a common difficulty, which is often

239 rotein, which undergoes a major irreversible refolding process to merge the two membranes.

240 n (F) protein, the latter undergoing a major refolding process to merge the two membranes.

241 suggests a kinetic coupling of the unfolding/refolding process with cis-trans prolyl isomerization.

242 to undergo a reversible pH-induced unfolding/refolding process, a loss/gain of alpha-helical structur

243 residues function as hinge positions in the refolding process, which closes the secondary ligand sph

244 known about the intermediate states of the F refolding process.

245 The associated refolding processes often cannot be explained by thermod

246 protein chaperone with well-defined peptide-refolding properties, is known to interact with ARE-like

247 for dynamic, interconverting, unfolding, and refolding proteins.

248 sed cell-free protein synthesis and a simple refolding protocol.

249 dest yield but required the incorporation of refolding protocols to obtain a proper conformation.

250 The unstressed refolding rate of MSD is ~17 s(-1) and slows exponential

251 d-type proteins indicates the differences in refolding rates may be correlated with the degree of fru

252 through all-or-none transitions with similar refolding rates.

253 onential force dependencies of unfolding and refolding rates.

254 This indicates that the unfolding/refolding reaction is kinetically determined with differ

255 Here, we investigated the refolding reaction of ribonuclease T1 in the presence of

256 mutation in reconstituted disaggregation or refolding reactions in vitro.

257 This article discusses the structures and refolding reactions of specific fusion proteins and the

258 itiate thermodynamically favorable unfolding-refolding reactions that release the (DMA)C-labeled stra

259 rces, bringing the non-equilibrium unfolding-refolding reactions towards equilibrium.

260 at belong to the same supertype, and, during refolding, reduced aggregation of tapasin-independent al

261 Notably, a pH-dependent refolding region (residues 824-858) at the spike-interdo

262 e prevention of hinge movements in the first refolding region and the elimination of proteolytic expo

263 isfolds and how chaperones assist Luciferase refolding remains unknown.

264 ive conformation thus requires unfolding and refolding, resulting in a long-lived intermediate.

265 kinetic analysis of human carbonic anhydrase refolding showed that 6AP decreased the yield of the ref

266 50 mus of all-atom molecular dynamics P-drop refolding simulations with four different force fields.

267 -type and adapted HIV-1 isolates, early gp41 refolding steps obligatorily occur on cell surfaces, whe

268 antibodies in engineered cells often require refolding steps or secretion across one or more biologic

269 and purified protein after simple oxidative refolding steps retained reduction-sensitive conformatio

270 duced unfolding experiments and stopped-flow refolding studies at ambient pressure, NMR-detected pres

271 nsive mechanism to promote RNA unfolding and refolding, suggesting an evolutionary convergence with p

272 cule atomic force microscopy (AFM) unfolding/refolding techniques to study the interactions of the UC

273 /mol that represents the free energy cost of refolding the oligomeric intermediate into the structure

274 and facilitates its ER-to-cytosol export by refolding the toxin as it emerges at the cytosolic face

275 ge of folding newly synthesized proteins and refolding those that have become misfolded in the contex

276 nce similarity but all are thought to act by refolding through a series of conformational intermediat

277 ribosomal state to allow for mRNA structure refolding to drive large-scale ribosome movements.

278 nts that facilitate their flexible C-termini refolding to engage distinct interfaces.

279 ormational changes that energetically couple refolding to membrane fusion.

280 ase, we observe a slower 1.4-ms phase during refolding to the native state.

281 eins from the aggregates and assist in their refolding to the native state.

282 s F structures demonstrates that a conserved refolding trajectory mediates entry of these viruses and

283 tandem hairpins displayed ensemble unfolding/refolding transitions, which were exploited to recognize

284 The solid support allows for multiple refolding trials through buffer exchanges, and the EPR s

285 tion complex, or the RNA undergoes extensive refolding upon encapsidation.

286 events their aggregation, and supports their refolding upon subsequent neutralization.

287 Refolding was confirmed by ultrafast broadband transient

288 and hysteresis in the thermal unfolding and refolding was observed for all proteins.

289 Refolding was rapid and stochastically redistributed mol

290 ding states of cyt c in the early 500 mus of refolding was revealed on the microsecond time scale.

291 lysis that occurs in the absence of ribozyme refolding, we find that approximately 100 ATPs are hydro

292 ing the rates of ATP hydrolysis and ribozyme refolding, we find that several hundred ATP molecules ar

293 lling speeds (ca. 2 nm s(-1)), unfolding and refolding were observed to occur in near equilibrium.

294 The best conditions for refolding were optimized by a high throughput screening

295 iv) characterize the early stages of protein refolding when chemically denatured proteins are transfe

296 on the assumption of DSB-induced chromosome refolding, which also takes into account the previously

297 the DSB is induced chromosome III undergoes refolding, which directs the MAT locus to recombine with

298 ) at loads <100 pN were accompanied by rapid refolding without either intra- or interhelix unfolding

299 EPR spectra at each state of the refolding workflow of spin-labeled Haloarcula marismortu

300 he most beneficial factors for improving the refolding yield.