ライフサイエンスコーパス: replication complex

1 sDNA sequences within the functioning T4 DNA replication complex.

2 pology of the Beet black scorch virus (BBSV) replication complex.

3 nality of specific proteins within the virus replication complex.

4 ay regulate the assembly of an active genome replication complex.

5 in cultured cells, and may be present in the replication complex.

6 ed following stress, it redistributes to the replication complex.

7 idylinositol 4-phosphate (PI4P) lipid to the replication complex.

8 ion and retargeting of the PI4P lipid to its replication complex.

9 component of the bacteriophage T4-coded DNA replication complex.

10 otency and cancer, and as a component of the replication complex.

11 C protein is an additional component of the replication complex.

12 er in the absence of other components of the replication complex.

13 lar interactions that occur within the viral replication complex.

14 endomembrane and facilitate formation of the replication complex.

15 ance (MCM) proteins, members of the cellular replication complex.

16 ion, including the formation of a functional replication complex.

17 s a central role in the formation of the HCV replication complex.

18 s-acting function that may occur outside the replication complex.

19 mpair the function of the EBOV transcription/replication complex.

20 ant component of the hepatitis C virus (HCV) replication complex.

21 est that this may represent a coxsackievirus replication complex.

22 is an integral component of viral lytic DNA replication complex.

23 ting the expression of components of the pre-replication complex.

24 ructural proteins (nsps) that form the viral replication complex.

25 imicking the form that would be present in a replication complex.

26 eeps the primer in physical proximity to the replication complex.

27 oteins recruited to the origins form the pre-replication complex.

28 l protein 3 (nsp3), a component of the viral replication complex.

29 viral components required to form the viral replication complex.

30 vity against DENV, and localizes to the DENV replication complex.

31 is a member of, and can regulate, the HPV16 replication complex.

32 erative binding of gp32 molecules within the replication complex.

33 lymerase (RdRp) 3D are involved in the viral replication complex.

34 y active 3D molecules and those that build a replication complex.

35 ment synthesis behind and independent of the replication complex.

36 targeting a cellular component of the viral replication complex.

37 interact with other components of the T4 DNA replication complex.

38 n and microenvironment of plant (+)RNA virus replication complexes.

39 l DNA replication, and failure to form viral replication complexes.

40 lar machinery to form and operate membranous replication complexes.

41 lication as well as real-time measurement of replication complexes.

42 ltimately becomes associated with functional replication complexes.

43 ate with or assist in the formation of viral replication complexes.

44 ing events downstream of the assembly of pre-replication complexes.

45 pparent role in promoting progression of DNA replication complexes.

46 y acid biosynthetic pathway to establish its replication complexes.

47 uctural protein 3 (nsp3), a marker for virus replication complexes.

48 for genome replication, perhaps formation of replication complexes.

49 embrane anchors during assembly of the viral replication complexes.

50 olism could still be fully functional in the replication complexes.

51 alterations leading to the formation of HCV replication complexes.

52 y involve release of replicated genomes from replication complexes.

53 r sites, consistent with membrane-associated replication complexes.

54 proteins into formation of functional viral replication complexes.

55 lum-derived motile vesicles containing viral replication complexes.

56 roteins, which mediate the assembly of viral replication complexes.

57 BF1, likely mediating its recruitment to the replication complexes.

58 t poorly understood membrane-bound viral RNA replication complexes.

59 entry, with incoming virions failing to form replication complexes.

60 he majority of the dsRNA was associated with replication complexes.

61 owing to loss of functional connection with replication complexes.

62 P3 proteins recruit host proteins into viral replication complexes.

63 The largest viral protein in the replication complex, 2C, is thought to have multiple rol

64 en envelope proteins and NS3 and NS5A within replication complexes adjacent to lipid droplets, where

65 n uninfected cells and with the dengue virus replication complex after infection.

66 not only an essential component of the viral replication complex and a prime target for antiviral int

67 nction that likely occurs as part of the HCV replication complex and a trans-acting function that may

68 endent RNA polymerase form part of the viral replication complex and are involved in viral RNA genome

69 tent with the tight juxtaposition of the HCV replication complex and assembly site at the LD surface.

70 g with MOV10 and Xrn1, localizes to the DENV replication complex and associates with DENV proteins.

71 PI4KIIIalpha results in a breakdown of this replication complex and cessation of HCV replication in

72 NS3 is intimately associated with the viral replication complex and dsRNA.

73 lication licensing factor is part of the pre-replication complex and essential for the maintenance of

74 raction abolishes the formation of the HPV16 replication complex and impairs HPV16 DNA replication in

75 ular enzyme, SIRT1, is part of the HPV16 DNA replication complex and is brought to the viral genome b

76 SIRT1 is part of the E1-E2 DNA replication complex and is recruited to the viral origin

77 pid-binding protein, is recruited to the HCV replication complex and is required for HCV genome repli

78 nsistent with the tight juxtaposition of the replication complex and the assembly site at the surface

79 ously shown to contain elements of the viral replication complex and the TGN resident endoprotease Ke

80 (MNV), is intimately associated with the MNV replication complex and the viral replication intermedia

81 FMDV protein 2C is part of the replication complex and thought to have multiple roles d

82 C) complex, may interact with incoming HIV-1 replication complexes and affect key steps of infection.

83 ion disrupts the integrity of membranous HCV replication complexes and renders HCV RNA susceptible to

84 hitectural basis for nuclear homing of HIV-1 replication complexes and subsequent integration into as

85 ique lipid blueprints on which they assemble replication complexes and synthesize viral RNA.

86 ts the development and/or functioning of the replication complexes and that Arf6 plays a previously u

87 onment that supports the formation of active replication complexes and the initiation of RNA synthesi

88 A mcm5-bob1 mutant that de-regulates the pre-replication complex, and again saw no change in telomere

89 are two important components of the HCV RNA replication complex, and nascent HCV RNA to autophagosom

90 sing of DNA replication by re-activating pre-replication complexes, and DNA repair.

91 ropagation of the viral genome occurs within replication complexes, and understanding this process ca

92 if impaired polymerase activity, assembly of replication complexes, and viral replication.

93 ractions with other components of the T4 DNA replication complex are discussed.

94 ngs indicate that components of the reovirus replication complex are mediators of cell-selective vira

95 t factors in the hepatitis C virus (HCV) RNA replication complex are not well understood.

96 For poliovirus, these replication complexes are anchored to the membrane via t

97 Our results showed that Nodamura virus replication complexes are targeted to mitochondria, as e

98 parate localization of NS1 and the viral RNA replication complex, as the latter is present on the cyt

99 proteins, which promotes the assembly of the replication complexes, as well as cellular poly(A) bindi

100 Hsp90 and Hsp70 chaperone systems in FHV RNA replication complex assembly and function in Saccharomyc

101 level of redundancy in using host factors in replication complex assembly and virus replication.

102 leating center for ORC assembly and then pre-replication complex assembly by binding to mitotic chrom

103 To study viral replication complex assembly we use the established mode

104 enomic replicon offers a unique way to study replication complex assembly, as it enables improved com

105 In addition to replication complex assembly, the results have implicati

106 ane interactions, an initial step of FHV RNA replication complex assembly, we established an in vitro

107 To better understand replication complex assembly, we studied the mechanisms

108 in, is thought to function as a scaffold for replication complex assembly; however, functional intera

109 -strand RNA genomes amplify their genomes in replication complexes associated with cellular membranes

110 Although all precursors could cleave a replication complex-associated substrate, only a subset

111 is that K-Rta facilitates the formation of a replication complex at oriLyt.

112 exact mechanism for de novo assembly of the replication complex at the replication origin is, or how

113 ulates viral gene transcription, nucleates a replication complex at the viral origin of lytic replica

114 uired in vitro for pol eta to gain access to replication complexes at forks stalled by T (wedge)T and

115 n 6 (Cdc6) is essential for formation of pre-replication complexes at origins of DNA replication.

116 n, in which MPs function to compartmentalize replication complexes at PD for localized RNA synthesis

117 nto the microscopic details of DDR factor/LT replication complexes at the MCPyV origin but also provi

118 In addition to inhibiting replication complex biogenesis, daclatasvir prevents vir

119 mage induces assembly and execution of a DNA replication complex (break-induced replisome) at telomer

120 that CPSF6 is strongly recruited to nuclear replication complexes but absent from cytoplasmic RTC/PI

121 l ORF1a protein nsp10 colocalizes with viral replication complexes, but its role in transcription/rep

122 Subsequently, NS3 is recruited to the replication complex by NS5BDelta21, resulting in RNA syn

123 Hsc70 associates with the HCV replication complex by primarily binding to the poly U/U

124 d membranes facilitate the assembly of viral replication complexes by sequestering viral and co-opted

125 Our data lead to a model wherein the replication complex can be assembled with a minimally ac

126 y the APC/C-dependent oscillation of the pre-replication complex component Orc1.

127 emonstrate that fusion to the membrane-bound replication complex components permits efficient cleavag

128 The picornavirus replication complex comprises a coordinated network of p

129 ), as an essential host component of the HCV replication complex consisting of NS5A, the RNA-dependen

130 te marker of the rate of intracellular viral replication complex decay.

131 se that phosphorylates components of the pre-replication complex during DNA replication initiation.

132 eractions of the other sub-assemblies of the replication complex during the replication cycle.

133 eplication requires the recruitment of a pre-replication complex facilitated by Origin Recognition Co

134 tructure to remain in close proximity to the replication complex followed by reassembly on nascent DN

135 Assembly of virus replication complexes for all known positive-strand RNA

136 using cells lacking D-type cyclins, in which replication complexes form normally, and correspondingly

137 phila cells, Flock House nodavirus (FHV) RNA replication complexes form on outer mitochondrial membra

138 oteins that play multiple roles in viral RNA replication complex formation and function.

139 will help us to understand the mechanism of replication complex formation and the pathogenesis of No

140 fore provides the first link between NoV RNA replication complex formation and the pathogenesis of th

141 -replicase fusions can be used to quantitate replication complex formation and virus replication.

142 ts establish RNA recruitment to the sites of replication complex formation as an essential, distinct,

143 Little is known about the mechanism of replication complex formation in cells infected with Nod

144 Our data indicate that Sindbis virus replication complex formation in mosquito cells activate

145 o a region of VP35 IID that is important for replication complex formation through interactions with

146 basic patch residues are likely critical for replication complex formation through interactions with

147 er-235 phosphorylation probably promotes the replication complex formation via increasing NS5A intera

148 ganization but show important differences in replication complex formation, cell entry, host tropism,

149 d not affect the establishment of infection, replication complex formation, the synthesis of nonstruc

150 modify host cytoplasmic membranes for viral replication complex formation.

151 nting an early stage in the steps leading to replication complex formation.

152 d is required for HCV genome replication and replication complex formation.

153 f PI4KA-dependent PI4P production influences replication complex formation.

154 he outer mitochondrial membrane sites of RNA replication complex formation.

155 ation of DNA replication, but not in the pre-replication complex formation.

156 e interface can explain potent inhibition of replication-complex formation, resistance, effects on li

157 cycle, some of which can be supplied to the replication complex from a separate genome (i.e., in tra

158 , and the autophagy pathway facilitates IBDV replication complex function and virus assembly, which i

159 r of the host proteins involved in the viral replication complex have been identified, including oxys

160 that E7(2) reduced the amount of GBF1 on the replication complexes; however, the level of PI4KIIIbeta

161 of these genes encode components of the pre-replication complex, implicating defects in replication

162 lication and the subcellular localization of replication complexes.IMPORTANCE Hepatitis C virus is an

163 A, we demonstrated the role of the viral RNA replication complex in efficient replication of viruses

164 It forms a replication complex in the presence of replication facto

165 tein (G3BP) and sequesters it into viral RNA replication complexes in a manner that inhibits the form

166 imaged nuclear entry and transport of HIV-1 replication complexes in cell lines, primary monocyte-de

167 sly established system for visualizing MCPyV replication complexes in cells.

168 d, positive-sense RNA viruses assemble their replication complexes in infected cells from a multidoma

169 mini-chromosome maintenance complex onto pre-replication complexes in late S, G(2), and M phases.

170 gets the checkpoint kinase Rad53 to distinct replication complexes in the budding yeast Saccharomyces

171 both within the virus-induced membrane-bound replication complexes in the host cytoplasm and in the n

172 SL] RNA (37) and colocalized with flavivirus replication complexes in WNV- and dengue virus-infected

173 r (400 mg/day), and daclatasvir, an HCV NS5A replication complex inhibitor (60 mg/day), were co-admin

174 ity in HCV-infected subjects by the HCV NS5A replication complex inhibitor (RCI) daclatasvir (1) spaw

175 vided clinical proof-of-concept for the NS5A replication complex inhibitor class, and regulatory appr

176 aprevir (64), marketed as Sunvepra, the NS5A replication complex inhibitor daclatasvir (117), markete

177 the identification of the highly potent NS5A replication complex inhibitor daclatasvir (33) are descr

178 first-in-class hepatitis C virus (HCV) NS5A replication complex inhibitor daclatasvir (6) provides a

179 ndomly assigned patients to receive the NS5A replication complex inhibitor daclatasvir (60 mg once da

180 BMS-7590052 is the first NS5A replication complex inhibitor with multiple dose proof-o

181 ssed all-oral therapy with daclatasvir (NS5A replication complex inhibitor) plus asunaprevir (NS3 pro

182 We evaluated daclatasvir (an HCV NS5A replication complex inhibitor) plus sofosbuvir (a nucleo

183 antivirals, comprising daclatasvir (an NS5A replication complex inhibitor), asunaprevir (an NS3 prot

184 ial of the combination of ombitasvir (a NS5A replication complex inhibitor), paritaprevir, and ritona

185 The NS5A replication complex inhibitor, BMS-790052, inhibits hepa

186 y of the HCV nonstructural protein 5A (NS5A) replication complex inhibitor, BMS-790052, was investiga

187 MS-790052, a nonstructural protein 5A (NS5A) replication complex inhibitor, were evaluated in a doubl

188 HCV NS5A replication complex inhibitors are pan-genotypic in spec

189 nd 3 are prototypes of a novel class of NS5A replication complex inhibitors that demonstrate high inh

190 NS5A replication complex inhibitors, exemplified by daclatasv

191 ons elucidated a high-resolution view of the replication complexes inside vesicles and allowed us to

192 the most well-studied components of the HCV replication complex is a helicase known as nonstructural

193 ng the formation of the membrane-bound viral replication complex is a major frontier in current virol

194 ed during the S phase, is present in the DNA replication complex isolated from human cells, with enha

195 that the level of recruitment of GBF1 to the replication complexes limits the establishment and expre

196 that this viral protein, a marker for viral replication complexes, localized in the extracellular sp

197 sDNA loop was frequently associated with the replication complex located at one end of the replicated

198 p16 and possibly other subunits of the viral replication complex may be a target for the development

199 we uncovered conserved interactions between replication complex members that modify the localization

200 Together with other components of the genome replication complex (NS3, double-stranded RNA, and cellu

201 Replication complexes of (+)RNA viruses of eukaryotes ar

202 d demonstrate active translation proximal to replication complexes of several coronaviruses.

203 It has been shown previously that replication complexes of some other positive-strand RNA

204 Hepatitis C virus (HCV) assembles its replication complex on cytosolic membrane vesicles often

205 umulation leading to the assembly of the pre-replication complex on DNA.

206 , which robustly enhanced the formation of a replication complex on the LD-associated endoplasmic ret

207 ruses is the necessity to assemble viral RNA replication complexes on host intracellular membranes, a

208 The assembly of RNA replication complexes on intracellular membranes is an e

209 sibly facilitating the assembly of viral RNA replication complexes on the cytoplasmic face of intrace

210 Flock House virus (FHV), which assembles its replication complexes on the mitochondrial outer membran

211 hase, presumably to prevent reloading of pre-replication complexes once S-phase has begun.

212 utations disrupted the formation of putative replication complexes, one mutation altered the stabilit

213 r the RNA is packaged as it emerges from the replication complex, or the RNA undergoes extensive refo

214 D40 repeat domain, interacts with the origin replication complex (ORC), a protein complex involved in

215 Previous studies of coronavirus replication complex organization and protein interaction

216 d to maintain genomic stability and may help replication complexes overcome sites of damaged DNA and

217 that MMR may have a "special relation to the replication complex", perhaps one that allows 5' or 3' D

218 %) that is replicated by the nuclear-encoded replication complex Pfprex.

219 In this study, we used preinitiation RNA replication complexes (PIRCs) to determine when CRE-depe

220 These results argue that the replication complex plays a role in modulating the SOS r

221 ng single-molecule experiments, we show that replication complexes pre-assembled on DNA support synth

222 yclin E has been shown to have a role in pre-replication complex (Pre-RC) assembly in cells re-enteri

223 y promotes cdc6 and cdt1 expression, and pre-replication complex (pre-RC) assembly in cycling cells.

224 to investigate the link between zones of pre-replication complex (pre-RC) assembly, replication initi

225 Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-as

226 ole in orchestrating the assembly of the pre-replication complex (pre-RC) by ORC-Cdc6 and Cdt1.

227 Since the assembly of pre-replication complex (pre-RC) in G(1) is required for the

228 that HOXC10 regulates the expression of pre-replication complex (pre-RC) proteins in sensitive tumor

229 data revealed asymmetric localization of pre-replication complex (pre-RC) proteins within large NDRs-

230 hromatin together with components of the pre-replication complex (pre-RC), such as the minichromosome

231 on requires the assembly of multiprotein pre-replication complexes (pre-RCs) at chromosomal origins o

232 o humans and is required for assembly of pre-replication complexes (pre-RCs) to initiate DNA replicat

233 on is due to a defect in the assembly of pre-replication complexes (pre-RCs).

234 mutations in multiple components of the pre-replication complex (preRC; ORC1, ORC4, ORC6, CDT1, or C

235 The pre-replication complex protein, origin recognition complex-

236 Genome-scale mapping of pre-replication complex proteins has not been reported in ma

237 mutations in one of five genes encoding pre-replication complex proteins: ORC1, ORC4, ORC6, CDT1, an

238 Interestingly, FAPP2 is redistributed to the replication complex (RC) characterized by HCV NS5A, NS4B

239 A occurs on intracellular membranes, and the replication complex (RC) contains viral RNA, nonstructur

240 toichiometrically participates in flavivirus replication complex (RC) formation is unknown; both redu

241 argeted to sites of yellow fever virus (YFV) replication complex (RC) formation, where it interacts w

242 ith other viral and host proteins within the replication complex (RC), and regulatory elements within

243 in the organization and function of the HCV replication complex (RC).

244 plicates its genome in a membrane-associated replication complex (RC).

245 he host cell's secretory pathway to generate replication complexes (RCs) for viral RNA synthesis.

246 Here we show that NoV establishes RNA replication complexes (RCs) in association with mitochon

247 eplicates its RNA within membrane-associated replication complexes (RCs) in the cytoplasm of infected

248 , the complex of P123+nsP4 forms the primary replication complexes (RCs) that function in negative-st

249 embranous structures in the cytoplasm called replication complexes (RCs).

250 perates and whether it is a component of the replication complex remained unclear.

251 , and yet its biological function within the replication complex remains unknown.

252 pment of specialized domains harboring viral replication complexes, replication organelles.

253 yclin E leads to inefficient assembly of pre-replication complexes, replication stress, and chromosom

254 Collisions between DNA replication complexes (replisomes) and barriers such as

255 Frequent collisions between cellular DNA replication complexes (replisomes) and obstacles such as

256 DNA replication complexes (replisomes) routinely encounter p

257 th detergent-resistant membranes where viral replication complexes reside.

258 ectron microscopy (cryo-EM) of nodavirus RNA replication complexes revealed that the viral double-str

259 reviously undescribed aspect of picornavirus replication complex structure-function and an important

260 suggest that the coordinated action of these replication complexes supports leading strand synthesis.

261 n the nature of the minicircle template, the replication complex synthesized leading and lagging stra

262 el mechanism of NHC interaction with the CoV replication complex that may shed light on critical aspe

263 ure products, P150 and P90, that compose the replication complex that mediates viral RNA replication;

264 ge is a central integrating component of the replication complex that must continuously bind to and u

265 tion of the virus occurs in association with replication complexes that are formed by host cell membr

266 pothesis that Nodamura virus establishes RNA replication complexes that associate with mitochondria i

267 man cells, SFTSV sequesters STAT proteins in replication complexes, thereby inhibiting type I interfe

268 Nuclear entry of HIV-1 replication complexes through intact nuclear pore comple

269 elements that function as a part of the pre-replication complex to initiate DNA replication in eukar

270 allows NS2A to recruit nascent RNA from the replication complex to the virion assembly site.

271 ymerase, functions in TLS and allows for DNA replication complexes to bypass lesions in DNA.

272 that TMEM41B is recruited to flavivirus RNA replication complexes to facilitate membrane curvature,

273 these, DnaJB11 relocalizes to virus-induced replication complexes to promote RNA synthesis, while Dn

274 We show that viral replication complexes traffic to and accumulate within n

275 embrane metabolism and is recruited to viral replication complexes upon infection.

276 of HCV proteins because viral particles and replication complex vesicles cannot form or egress.

277 embranous web structures and, ultimately, of replication complex vesicles, but also inhibits an early

278 aracteristic inclusion bodies known as viral replication complexes (VRCs), with a second population o

279 e the formation of host membrane-bound viral replication complexes (VRCs), yet the underlying mechani

280 (HVD) in EEEV nsP3 for the assembly of viral replication complexes (vRCs).

281 and play critical roles in assembly of viral replication complexes (vRCs).

282 ns in infected cells in order to build viral replication complexes (VRCs).

283 ost factors and various lipids to form viral replication complexes (VRCs).

284 ining replication-associated proteins (virus replication complexes [VRCs]) upon infection.

285 formation of the membranous cytoplasmic MNV replication complex, where Atg16L1 localized.

286 how that ORC1--a component of ORC (origin of replication complex), which mediates pre-DNA replication

287 acilitates the formation of virus-associated replication complexes, which are required for the amplif

288 by strictly controlling the activity of pre-replication complexes, which assemble at specific sites

289 sites of single strand lesions in the DNA of replication complexes, which has a more open structure c

290 olocalized at the actively replicating MCPyV replication complexes, which were absent when a replicat

291 ed both the formation and functioning of the replication complexes, while E5(1) and E7(2) were most e

292 tural 5A protein (NS5A) and enriches the HCV replication complex with its product, phosphoinositol 4-

293 ded DNA binding protein (gp32) of the T4 DNA replication complex with longer ssDNA (and dsDNA) lattic

294 The association of the HCV RNA replication complex with the autophagosomal membranes wa

295 biophysical measurements on T4 bacteriophage replication complexes with detailed structural informati

296 e cells which would additionally contain DWV replication complexes with negative-strand DWV RNA.

297 ed to stabilize the association of the viral replication complexes with nuclear speckles.

298 aps of the subcellular localization of viral replication complexes with the RNAi machinery, and the r

299 The coupling of membrane-associated replication complexes with virus intra- and intercellula

300 C to direct staged assembly of bacterial pre-replication complexes, with DnaA contacting low affinity