ライフサイエンスコーパス: replication origin

1 crete sites in the genome, termed origins of replication (origins).

2 gnition complex (Orc1-6; ORC) recognizes the replication origin.

3 head-to-head hexameric helicases around the replication origin.

4 e host cell DNA replication machinery to the replication origin.

5 cells directs MukBEF colocalization with the replication origin.

6 III (Pol III) holoenzyme association at the replication origin.

7 The SNP maps directly within the replication origin.

8 nsertion of an OGRE/G4 element created a new replication origin.

9 loaded at centromeric sites adjacent to the replication origin.

10 the genome-wide identification of human DNA replication origins.

11 oes so in close proximity to the pre-defined replication origins.

12 dentification of only a handful of mammalian replication origins.

13 d a 26-bp sequence related to alphasatellite replication origins.

14 tion initiation at a distinct group of human replication origins.

15 to compensation through increased firing of replication origins.

16 tivation of MCM helicase complexes loaded at replication origins.

17 the local chromatin environment surrounding replication origins.

18 ncomitant increase in the utilization of new replication origins.

19 cytoplasm of VACV-infected cells to pinpoint replication origins.

20 to enhanced loading of Cdc45 onto potential replication origins.

21 lication without activating a novel group of replication origins.

22 ate and severe defect in the partitioning of replication origins.

23 iates from multiple genomic locations called replication origins.

24 de insight into the nature of eukaryotic DNA replication origins.

25 nt response to inhibit further firing of DNA replication origins.

26 restart while suppressing the firing of new replication origins.

27 s from p53-/- mice and inhibit firing of DNA replication origins.

28 ng RNAs, promoters, regulatory sequences and replication origins.

29 t loads the Mcm2-7 replicative helicase onto replication origins.

30 otic DNA replication initiates from multiple replication origins.

31 d imply that the repetitive monomers contain replication origins.

32 tic genomes are replicated from multiple DNA replication origins.

33 ly occurs within a nucleosome-free region at replication origins.

34 by definition, include at least a subset of replication origins.

35 in the association of DDK with MCM rings at replication origins.

36 cation forks while suppressing firing of new replication origins.

37 he DDK- and CDK-dependent activation of late replication origins.

38 vation of the Cdc45-MCM-GINS helicase at DNA replication origins.

39 d the Mcm2-7 replicative helicase complex at replication origins.

40 icensing, which entails loading MCM-2-7 onto replication origins.

41 th replication forks emanating from multiple replication origins.

42 eplication forks and increased firing of new replication origins.

43 CM7 to DNA and thereby impairs the firing of replication origins.

44 forks thereby suppressing the firing of new replication origins.

45 tion, and decreased association of GINS with replication origins.

46 defects, along with diminished RPA signal at replication origins.

47 eplicative helicase, into double hexamers at replication origins.

48 tes on loaded Mcm double hexamers at unfired replication origins [9, 10].

49 e to the dynamic selection and activation of replication origins across diverse cell types and develo

50 double hexamers and during S phase licensed replication origins activate to initiate bidirectional r

51 icate that NCOA4 acts as an inhibitor of DNA replication origin activation by regulating CMG (CDC45/M

52 lished the succession of events that lead to replication origin activation by the MCM and recent stud

53 Cdc7 kinase is required for replication origin activation, is a target of the intra-

54 sion, while preserving the relative order of replication origin activation.

55 ls in S phase and genome-wide impairments of replication origin activation.

56 DNA replication origin activity changes during development.

57 ch elements can independently associate with replication origin activity.

58 of the prereplicative complex at chromosomal replication origins after exposure to UV light (UVC).

59 ssentially conferred by TrfA2 and the intact replication origin alone but that TrfA1 is nonetheless i

60 DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuri

61 lthough G4-forming sequences are abundant in replication origins, an asymmetry in nucleotide distribu

62 tenance (MCM) helicase complexes at many DNA replication origins, an essential process termed origin

63 ntegration of cassettes containing the c-myc replication origin and (CTG)(n) . (CAG)(n) TNRs in HeLa

64 ents for anchoring polymerase at the plasmid replication origin and bring insights of how the directi

65 imal cis-element modules containing a strong replication origin and chromatin modifier binding sites

66 The NCCR contains the viral DNA replication origin and cis-acting elements regulating vi

67 both Orc1 and Cdc6 functions by binding to a replication origin and directly recruiting MCM helicase.

68 e chromatin, does not depend on the episomal replication origin and initiates at multiple single-stra

69 into the mechanism of EBNA1 function at the replication origin and new opportunities to inhibit EBV

70 e chromosome arms lie side-by-side, with the replication origin and terminus at opposite cell poles.

71 Interestingly, premutation hESCs have a replication origin and the T variant similar to nonaffec

72 Instead, fewer replication origins and a higher frequency of DNA breaks

73 DNA replicative helicase, has fewer dormant replication origins and an increased number of stalled r

74 their neighboring promoters are close to DNA replication origins and bind SA with proportional levels

75 e, through a comparative genomic analysis of replication origins and chromosomal replication patterns

76 ing of the MCM complex onto chromatin at the replication origins and decreased cyclin D1 levels, wher

77 ctions in the regulation of the licensing of replication origins and expanding the scope of its overa

78 T530-pSIRT1 associates with replication origins and inhibits replication from a grou

79 ern dynamics and spatial organization of DNA replication origins and possibly other functional DNA el

80 ryotic DNA synthesis initiates from multiple replication origins and progresses through bidirectional

81 tem cells to regulate the temporal firing of replication origins and quality control of replicated DN

82 We used natural budding yeast DNA replication origins and synthetic DNA sequences to show

83 and repair, including the identification of replication origins and termination regions.

84 The factors that define the locations of replication origins and their typical activation times i

85 defined by broad domains that span multiple replication origins and topological boundaries.

86 rrelate with sequence motifs associated with replication origins and with locations that are preferen

87 H4K20me2 is enriched at replication origins, and abrogating ORC1 recognition of

88 ne expression patterns, specification of DNA replication origins, and definition of chromatin domains

89 , cohesin is transiently recruited to active replication origins, and it spreads along DNA as forks p

90 ion events at a G-quadruplex region near the replication origin are thought to drive replication of m

91 During late mitosis and G1 replication origins are "licensed" by loading MCM2-7 dou

92 Replication origins are cis-acting elements that potenti

93 Transcription and DNA replication origins are correlated, but the association

94 Eukaryotic replication origins are defined by the ORC-dependent loa

95 complete genome replication is maximized if replication origins are evenly spaced, the largest inter

96 Collectively, our data indicate that replication origins are fundamental organizers and regul

97 Recent studies reveal that early DNA replication origins are important for determining which

98 During the gap between G1 and S phases when replication origins are licensed and fired, it is possib

99 Eukaryotic replication origins are licensed by the loading of the r

100 high resolution in C. elegans, we show that replication origins are marked with specific histone mod

101 DNA replication origins are necessary for the duplication of

102 ntrary to the bacterial paradigm, eukaryotic replication origins are not irrevocably defined by selec

103 sis I is distinct from mitotic exit, in that replication origins are not licensed by Mcm2-7 chromatin

104 control the location and timing of firing of replication origins are poorly understood.

105 Contrary to expectation, we find that replication origins are specified prior to the broad ons

106 Replication origins are under tight regulation to ensure

107 d by about 3-fold, suggesting that fewer DNA replication origins are used in E1A-expressing cells.

108 tress promotes the initiation of alternative replication origins as an apparent means of rescue by fo

109 The complex loads at replication origins as an inactive double-hexamer encirc

110 cy, cells lacking Mus81 use the same pool of replication origins as Mus81-expressing cells.

111 ssDNA revealed intergenic regions, including replication origins, as hot spots for replication stress

112 enopus laevis, we show that SSRP1 stimulates replication origin assembly on somatic chromatin by prom

113 ulating DNA replication and directly linking replication origin assembly, cell cycle duration and emb

114 nd MCM chromatin binding, allowing efficient replication origin assembly.

115 gin recognition complex (ORC) to establish a replication origin at one element of oriP, DS (dyad symm

116 scopic and biochemical studies implicating a replication origin at the end of the VACV genome.

117 K293) cells, we identified the HBoV1 minimal replication origin at the right-end hairpin (OriR).

118 By mapping replication origins at different developmental stages, w

119 Re-replication occurs from specific replication origins at six genomic loci, termed Drosophi

120 y cell cycle by the programmed activation of replication origins at specific times and chromosomal lo

121 at lysine 4, known to prevent firing of late replication origins at the early S phase.

122 al approaches to identify start sites of DNA replication (origins) based on the presence of defining

123 CRL4 is recruited to chromatin by the replication origin binding protein RepID/DCAF14/PHIP.

124 he nucleotide-binding domain of NLRs and DNA replication origin-binding Cdc6/Orc1 proteins.

125 on, which allows DnaA oligomerization at the replication origin but the association state remains unc

126 m2-7 complexes assemble and are recruited to replication origins, but are defective in helicase loadi

127 hly regulated process that is initiated from replication origins, but the elements of chromatin struc

128 yotes duplicate their genomes using multiple replication origins, but the organization of origin firi

129 of active CMG (Cdc45-MCM-GINS) helicases at replication origins by a set of conserved and essential

130 Here, we show that LMO2 is recruited to DNA replication origins by interaction with three essential

131 The current model suggests that replication origins compete for a limited pool of initia

132 undaries separate regions of similarly timed replication origins connecting the long-known effect of

133 erevisiae (38%), and contains abundant yeast replication origin consensus sites (ACS) evenly distribu

134 nscribed long genes, particularly at the DNA replication origins contained therein.

135 ion terminates when replisomes from adjacent replication origins converge.

136 ement (DUE)-binding protein (DUE-B) binds to replication origins coordinately with the minichromosome

137 results suggest that a T/C SNP located at a replication origin could contribute to the inactivation

138 The formation of new replication origins (cSDR) and repair of DNA double-stra

139 High replication origin density can be restored by somatic nu

140 However, mechanisms underlying high replication origin density formation coupled to rapid ce

141 Whether Mcm10 recruitment to replication origins depends on CMG helicase assembly has

142 ication proteins, and its recruitment to DNA replication origins depends on the two pre-replicative c

143 egration of small replication bubbles causes replication origin destruction.

144 Cellular DNA replication origins direct the recruitment of replicativ

145 Eukaryotic cells license each DNA replication origin during G1 phase by assembling a prere

146 lin-dependent kinases, known to activate DNA replication origins during firing, inhibits MDM2-mediate

147 ome maintenance (MCM2-7) helicase complex at replication origins during G1 phase as an inactive doubl

148 maintenance (MCM) complex is first loaded at replication origins during G1 phase, and then converted

149 d in part by sequestration of DDK at unfired replication origins during S phase.

150 re we identify a new indispensable bacterial replication origin element composed of a repeating trinu

151 ) into pre-replicative complexes at multiple replication origins ensures precise once per cell cycle

152 rtQTLs involved the differential use of replication origins, exhibited allele-specific effects o

153 fission yeast and shows that in human cells replication origins fire stochastically forming clusters

154 isplay increased sister chromatid exchanges, replication origin firing and chromosomal aberrations.

155 se complexes, required for initiation of DNA replication origin firing and ongoing DNA synthesis duri

156 s program is based on the regulation of both replication origin firing and replication fork progressi

157 pha regulatory subunit, becomes limiting for replication origin firing at high N/C ratio, and this in

158 tant DNA synthesis was due to an increase in replication origin firing that compensated for reduced f

159 of the spatial and temporal organization of replication origin firing, analyzed using single DNA mol

160 hese obstacles, which includes modulation of replication origin firing, of the architecture of replic

161 cellular processes, including suppression of replication origin firing, promotion of deoxynucleotide

162 We have shown that GOF p53 increases DNA replication origin firing, stabilizes replication forks,

163 cation timing is determined by the timing of replication origin firing, which involves activation of

164 reased amount of DNA-bound MCM7 and impaired replication origin firing.

165 of DNA replication fork slowing and reduced replication origin firing.

166 se replication elongation checkpoint and the replication origin-firing checkpoint induced by camptoth

167 Examples include replication origin firings, formation of chromatin loops

168 MCM complexes are recruited to replication origins for genome duplication.

169 nction of multiple spatially distributed DNA replication origins for its stable inheritance.

170 l molecules to reveal the location of active replication origins, fork direction, termination sites,

171 ding, results in dynamic relocalization of a replication origin from the nuclear periphery to the int

172 the nucleus through epigenetic regulation of replication origin function.

173 sequencing (NS-seq) is used to discover DNA replication origins genome-wide, allowing identification

174 ifferential replication timing of eukaryotic replication origins has long been linked with epigenetic

175 sms that control the spatial organization of replication origins have potential impacts for genome re

176 id replication does not require a functional replication origin; however, in the presence of competit

177 ssion is associated with increased firing of replication origins, impaired replication fork progressi

178 regulating topoisomerase recruitment to the replication origin.IMPORTANCE Human papillomaviruses aff

179 could contribute to the inactivation of this replication origin in FXS hESCs, leading to altered repl

180 the unwound DNA within the Escherichia coli replication origin in the helicase loading process, but

181 on in Escherichia coli cells with an ectopic replication origin in which highly transcribed rrn opero

182 tiates at multiple discrete regions known as replication origins in a dynamic yet regulated manner to

183 mosomes initiate DNA synthesis from multiple replication origins in a temporally specific manner duri

184 In several metazoans, the number of active replication origins in embryonic nuclei is higher than i

185 understanding the mechanisms that define DNA replication origins in eukaryotes.

186 s required for the clustering of a subset of replication origins in G1 phase and for the early initia

187 Here, we link localization of replication origins in G1 phase with Fkh1 activity, whic

188 ATR and Cdc7 kinase-dependent mechanisms at replication origins in human cells.

189 The full licensing of replication origins in late G1 is normally enforced by t

190 ate the assembly of MCM2-7 onto chromatin at replication origins in late mitosis and G(1) phase.

191 Conversely, the weakening of replication origins in repetitive regions suppresses the

192 , our findings support the existence of more replication origins in T. brucei than previously appreci

193 ion forks emerging from any one of the three replication origins in the Sulfolobus chromosome remain

194 Fkh1-dsm binds replication origins in vivo but fails to cluster them, s

195 We previously showed how the distribution of replication origins in yeasts promotes complete genome r

196 th young and old cells were characterized by replication origins including circles from unique region

197 itiation of DNA replication from RepID-bound replication origins, including the origin at the human b

198 n that emerges from the stochastic nature of replication origins initiation.

199 rectional loading of two ring helicases at a replication origin is achieved by strictly regulated and

200 whereas accumulating evidence indicates the replication origin is also affected.

201 o assembly of the replication complex at the replication origin is, or how the directionality of repl

202 osome maintenance proteins 2-7 (MCM2-7) onto replication origins is a prerequisite for replication fo

203 g of the Origin Recognition Complex (ORC) to replication origins is essential for initiation of DNA r

204 te with cohesin, and suggest that cohesin at replication origins is important for establishing both s

205 The specification of mammalian chromosomal replication origins is incompletely understood.

206 of the temporally coordinated activation of replication origins is the establishment of broad domain

207 A key feature of replication origins is their ability to control the onse

208 ure rates that are consistent with data from replication origin knockout experiments.

209 The separation of DNA replication origin licensing and activation in the cell

210 suggest that mTOR signaling may control DNA replication origin licensing and replisome stability the

211 7 genes, which is expected to compromise DNA replication origin licensing and result in elevated rate

212 This is believed to be achieved by limiting replication origin licensing and thereby restricting the

213 s as head-to-head double hexamers during DNA replication origin licensing is crucial for ensuring onc

214 product levels and proteins involved in DNA replication origin licensing may explain the deleterious

215 CDC6 is essential for eukaryotic replication origin licensing, however, it is poorly unde

216 Knockdown of the replication origin-licensing factor CDT1 eliminates both

217 explain how specific proteins recognize DNA replication origins, load the replicative helicase on DN

218 ed to repeat expansion in haplogroup D and a replication origin located approximately 53 kb upstream

219 Importantly, we found a replication origin located near an ORC1/CDC6 binding sit

220 The DNA sequence specificity of replication origins, mediated by the Orc4 alpha-helix, h

221 t subgroups of replication initiation sites (replication origins) modulate the ubiquitous replication

222 yeasts limits the availability of efficient replication origin modules to only a handful of species

223 However, different approaches to mapping replication origins, namely (i) sequencing isolated smal

224 In S. cerevisiae, replication origins occupy characteristic subnuclear loc

225 tion of a lacZalpha cassette proximal to the replication origin of the phage used to construct the li

226 bility of specific recognition motifs within replication origins of higher organisms.

227 ls, involving the assembly and activation at replication origins of the CMG (Cdc45-MCM-GINS) DNA heli

228 Helicase loading at a DNA replication origin often requires the dynamic interactio

229 accumulating especially at the heavy strand replication origin OH, in the ribosomal genes (12S and 1

230 l replication initiation outside of the main replication origin, OH.

231 genes (12S and 16S) and at the light strand replication origin OL.

232 ains, that in vitro stretches DNA to promote replication origin opening.

233 se regions are characterized by a paucity of replication origins or unusual DNA structures.

234 ced by loss of licensing control at cellular replication origins, or by viral protein-driven multiple

235 H4K20me2 in cells impairs ORC1 occupancy at replication origins, ORC chromatin loading and cell-cycl

236 ation-dependent replication at mitochondrial replication origin ori5 in hypersuppressive rho- cells.

237 DnaA monomers recognize the replication origin (oriC) by binding double-stranded DNA

238 fforts focused on recombination proteins and replication origin (oriC) degradation.

239 We found that in new offspring, chromosome replication origins (oriCs) are arranged in a three-dime

240 At replication origins, our data revealed asymmetric locali

241 3681 lysis vector derivatives with different replication origins (pBR, p15A, pSC101), resulting in pY

242 terodimer with a free TP that recognizes the replication origins, placed at both 5' ends of the linea

243 umulating evidence suggests that dormant DNA replication origins play an important role in the recove

244 The selection and firing of DNA replication origins play key roles in ensuring that euka

245 to recruit a single Cdt1-Mcm2-7 heptamer to replication origins prior to Cdt1 release and ORC-Cdc6-M

246 meres and chromosomal arm regions containing replication origins proximal to binding sites for Taz1,

247 esponsible polymerase, replication time, and replication origin proximity.

248 ins (MCMs), which constitute the core of the replication origin recognition complex, were among the m

249 The chromosomal replication origin region (ori) of characterised bacteri

250 are normally associated with the chromosome replication origin region (ori).

251 plication and the nature and location of the replication origins remain unknown.

252 ID is involved in an interaction between the replication origin (Rep-P) and the locus control region.

253 We suggest that replication origins, replication timing, and replication

254 idirectional replication from eukaryotic DNA replication origins requires the loading of two ring-sha

255 Genome-wide studies of DNA replication origins revealed that origins preferentially

256 ted at particular sites on the genome called replication origins (ROs).

257 tion, we then show in vitro that the minimal replication origin sequence elements are necessary and s

258 ble-stranded oligonucleotides containing the replication origin sequence without the parental TP.

259 This raises the question of what purpose replication origins serve and why they have evolved.

260 DNA replication origins serve as sites of replicative helica

261 tic mechanisms are important for determining replication origin sites in budding yeast, highlighting

262 ing studies, and to include the collation of replication origin sites in the fission yeast Schizosacc

263 cation initiator proteins to pericentromeric replication origins so that they initiate replication ea

264 tion initiates from defined locations called replication origins; some origins are highly active, whe

265 east, highlighting mechanistic principles of replication origin specification that are common among e

266 We show that chromatin enforces DNA replication origin specificity by preventing non-specifi

267 assay has been a valuable tool in dissecting replication origin structure and function.

268 d Lachancea waltii, we assess to what extent replication origins survived genomic change produced fro

269 e majority of the ORCA-bound sites represent replication origins that also associate with the repress

270 tin islands at regions corresponding to late replication origins that are sites of double-strand brea

271 plication in eukaryotic cells initiates from replication origins that bind the Origin Recognition Com

272 contained consensus sequences for autonomous replication origins that could explain their maintenance

273 In budding yeast, centromeres are flanked by replication origins that fire in early S phase.

274 dicate that NCOA4 acts as a regulator of DNA replication origins that helps prevent inappropriate DNA

275 e structure and function of the archaeal DNA replication origins, the proteins that define them, and

276 The recruitment of MCMs to the replication origins through LANA was demonstrated throug

277 o the identification of tens of thousands of replication origins throughout mammalian genomes, provid

278 Bioinformatic analysis detects DnaA-trios in replication origins throughout the bacterial kingdom, in

279 actions during homologous recombination, and replication origin timing and long-range origin clusteri

280 s transcription factors yet are defective in replication origin timing control.

281 g and thereby restricting the firing of each replication origin to once per cell cycle.

282 he QnrB expression level correlated with the replication origin to terminus (oriC/ter) ratio, indicat

283 origin firing that determine the ability of replication origins to accrue limiting factors and have

284 ose a hypothesis based on aberrant firing of replication origins to explain intragenic nonrecurrent r

285 romosome structure regulates the capacity of replication origins to initiate, very little is known ab

286 th various cellular activities) domains bind replication origins to license new rounds of DNA synthes

287 plasmid-based expression systems require DNA replication origins to maintain plasmids efficiently.

288 ome maintenance complex (MCM) loading at DNA replication origins to prepare for S phase, known as ori

289 al agent of sleeping sickness, localized its replication origins to the boundaries of multigenic tran

290 Bacteria use the replication origin-to-terminus polarity of their circula

291 ur upstream of FMR1, suggesting that altered replication origin usage combined with fork stalling pro

292 hted by the finding that the distribution of replication origins varies between differentiated cell t

293 operator systems and a synthetic repressible replication origin, we tracked the motion and segregatio

294 ypersensitivity to licensing inhibition when replication origins were removed.

295 ruits the MCM2-7 replicative helicase to the replication origin, where MCM2-7 is activated to initiat

296 it Nipped-B and cohesin to enhancers and DNA replication origins, whereas the MED30 subunit of the Me

297 We localized the minimal replication origin, which contains both NS1 nicking and

298 rect observation of stochastic firing of DNA replication origins, which differs from cell to cell.

299 of mammalian genomes starts at sites termed replication origins, which historically have been diffic

300 lication from a group of 'dormant' potential replication origins, which initiate replication only whe