ライフサイエンスコーパス: reporter gene

1 the specific promoter that is linked to the reporter gene).

2 heterodimers-mediated transactivation of GUS reporter gene.

3 active in the 3' untranslated region of the reporter gene.

4 3 to induce expression of a STAT3-responsive reporter gene.

5 ne expression in the controlled setting of a reporter gene.

6 ced expression of an Asc promoter-luciferase reporter gene.

7 NIH-3T3 cells transfected with a beta-klotho reporter gene.

8 e the NSs open reading frame replaced with a reporter gene.

9 g at the promoter, and the reactivation of a reporter gene.

10 ve to DSPP-Cerulean, an odontoblast-specific reporter gene.

11 tion, and activity of the NF-kappaB-specific reporter gene.

12 hat is placed between the synthetic gene and reporter gene.

13 s along with the increased expression of the reporter gene.

14 atural introns previously tested in the same reporter gene.

15 e promoter, TREalb, for a firefly luciferase reporter gene.

16 HYDROGENASE1 promoter::luciferase (ADH1-LUC) reporter gene.

17 cells carrying the beta-glucuronidase intron reporter gene.

18 ite of an identical constitutively expressed reporter gene.

19 ally infected woman and a NanoLuc luciferase reporter gene.

20 rotein (GFP) encoded DNA plasmid (pGFP) as a reporter gene.

21 on were added to the 5'-UTR of a fluorescent reporter gene.

22 at NHEJ in trans led to expression of a LEU2 reporter gene.

23 ns are based on high, stable expression of a reporter gene.

24 limitation, we introduce mammalian acoustic reporter genes.

25 he PEG10 promoter to drive the expression of reporter genes.

26 ) expressing both fluorescent and luciferase reporter genes.

27 ctable subgenomic replicons with and without reporter genes.

28 hmark' vector titres are achieved with inert reporter genes.

29 either the yeast Gal4 or firefly luciferase reporter genes.

30 ence, bioluminescence imaging, and human PET reporter genes.

31 uses expressing each of six commonly used RV reporter genes.

32 r neurogenin-2 promoters fused to luciferase reporter genes.

33 intervening sequences flanked by fluorescent reporter genes.

34 ant IAV expressing fluorescent or luciferase reporter genes.

35 se models: (1) a knockout with inserted lacZ reporter gene; (2) a C59S point mutant substitution and

36 gonist/antagonist at the h/mH(4)Rs [pIC(50) (reporter gene) 8.76/7.08; pIC(50)/pK(b) (beta-arrestin2)

37 as a partial agonist at the hH(3)R [pEC(50) (reporter gene) 8.77] and as an inverse agonist/antagonis

38 express the green fluorescent protein (GFP) reporter gene (abbreviated as G in virus designations) a

39 conditional hair cell-specific gene deletion/reporter gene activation in the inner ear.

40 through Cre recombinase-mediated fluorescent reporter gene activation only in Stra8-expressing cells,

41 e Gfi1(Cre) mice, both for gene deletion and reporter gene activation, these data are significant and

42 l MRTF/SRF inhibitor, markedly decreased SRF reporter gene activity and showed a greater inhibitory e

43 In the absence of Msi1 and miR130a and -206, reporter gene activity decreased, indicating that Msi1 e

44 viral latency locus showed high, persistent reporter gene activity in non-neuronal cells while an in

45 o, gga-miR-429 directly repressed luciferase reporter gene activity via binding to 3' untranslated re

46 2 inhibitors on TGF-beta1-induced migration, reporter gene activity, and Smad activation.

47 o suppress TGF-beta1-induced cell migration, reporter gene activity, and Smad3 activation.

48 he CREB transcription factor to repress CREB-reporter gene activity.

49 to suppress MAMP-triggered pFRK1-Luciferase reporter gene activity.

50 Mammalian acoustic reporter genes allow cells to be visualized at volumetri

51 optimized for enhanced output upstream of a reporter gene allowed detection of prostate cancer by ne

52 ts, and CpG/UpA-dinucleotide optimization of reporter genes allowed replication.

53 In addition, the use of reporter genes allows FACS-purification and tracking of

54 The expression of two reporter genes allows monitoring of viral inhibition by

55 re cloned upstream of a minimal promoter and reporter gene, alongside a unique barcode, and introduce

56 Reporter gene analyses verified that this effect is due

57 in situ hybridization experiments, promoter-reporter gene analyses, and ZmSUT1 localization studies

58 in glucose-depleted conditions, and in vivo reporter gene analysis indicated reduced expression of t

59 es of CRM function undetected by traditional reporter gene analysis.

60 ng of Jurkat T cells stably overexpressing a reporter gene and a gene encoding TREM2DAP12 fusion prot

61 Reporter gene and chromatin immunoprecipitation assays i

62 vity from the NtPDR1 promoter in situ with a reporter gene and found that, although NtPDR1 expression

63 Reporter gene and in silico transcription factor binding

64 onally synergize with FKBP51, as revealed by reporter gene and protein association analyses.

65 cting specific hybridoma cells with aequorin reporter gene and the bioluminescence activities of stab

66 We believe PKM2 shows great promise as a PET reporter gene and to date is the only example that can b

67 The promoter was fused with gusA reporter gene and was expressed in Arabidopsis and rice

68 required for transcription of both chimeric reporter genes and authentic chromosomal Rap1 enhancer-c

69 ences, reactive oxygen species (ROS) assays, reporter genes and bacterial population measurements.

70 atory effect of HBx both on extrachromosomal reporter genes and on hepatitis B virus transcription.

71 ad3, Smad-dependent activity of a luciferase reporter gene, and cell migration.

72 tations; expression of foreign, modified, or reporter genes; and even targeted gene disruptions.

73 We show that mRNA half-lives for a reporter gene are increased in both fast and slow Pol II

74 eractions (PPIs) into stable expression of a reporter gene are useful for genetic selections, high-th

75 n, target bacteria become identifiable since reporter genes are expressed from the engineered phage g

76 We found that reporter genes are expressed to higher levels from the N

77 We show that the reporter genes are strongly induced in a cell density-de

78 e limitations associated with the use of one reporter gene as a readout.

79 miRNA molecules, which repress a luciferase reporter gene as well as endogenous mRNA targets of the

80 antioxidant response element beta lactamase reporter gene assay (ARE-bla), which identifies chemical

81 sing an estrogen response element-luciferase reporter gene assay and quantitative polymerase chain re

82 nstream gene Hes1 promoter-driven luciferase reporter gene assay and Western blotting showed that rec

83 n a quantitative high-throughput, cell-based reporter gene assay for TR agonist or antagonist activit

84 is a target of miR-34a by a dural luciferase reporter gene assay in vitro.

85 ve stress using antioxidant response element reporter gene assay models and big data.

86 Screening 23dd in a cell-based TCF/LEF reporter gene assay restored the activation of Wnt signa

87 Transactivation studies with a reporter gene assay revealed that gmAhr1a is one order o

88 By employing a pregnane X receptor (PXR) reporter gene assay to prioritize compounds for further

89 A fragment-based screening, reporter gene assay, and pharmacophore search were utili

90 d potent transcriptional activities in a VDR reporter gene assay, and significantly ameliorated cardi

91 the hit compound, identified in a NF-kappaB reporter gene assay, led to compound 9b, exhibiting a ce

92 Using a reporter gene assay, we demonstrated that C/P efficiency

93 ter sensitivity as compared to a traditional reporter gene assay.

94 human GR were investigated using luciferase reporter gene assay.

95 monstrated regulating enhancer activity in a reporter gene assay.

96 ne monophosphate response element-luciferase reporter gene assay.

97 d previously for mixture effects by in vitro reporter gene assays and were in this study analyzed usi

98 ithin transcription factor binding sites and reporter gene assays confirmed that they affect gene exp

99 tions in clock genes, RNA-seq, ChIP-seq, and reporter gene assays coupled with measurements of DNA-pr

100 Likewise, reporter gene assays demonstrated significantly lower GU

101 ChIP and reporter gene assays demonstrated that the -82 kb HRE re

102 Reporter gene assays demonstrated the ability of ICP0 to

103 lar analyses by gene expression analysis and reporter gene assays indicated an effect of rs385076 on

104 Transcriptome sequencing (RNA-seq) and reporter gene assays may be useful for testing engineere

105 Reporter gene assays revealed cyclin-dependent kinase 3

106 Bioassay analysis using cell-based reporter gene assays revealed estrogenic, androgenic, an

107 Reporter gene assays show that PecS-mediated repression

108 In reporter gene assays, all three variants increased CXCL4

109 As shown in cell-based dual-luciferase reporter gene assays, functional interaction occurred be

110 an array of different techniques, including reporter gene assays, immunocytochemistry, and ChIP-qPCR

111 quantitative real-time PCR, immunostaining, reporter gene assays, RNA-Seq, and two-photon glutamate

112 Using multiple luciferase reporter gene assays, we could demonstrate that editing

113 -PCR, viral infection and plaque assays, and reporter gene assays, we demonstrate that a microRNA clu

114 pitation, siRNA-mediated gene silencing, and reporter gene assays, we demonstrate that PKA activation

115 ied proteins, surface plasmon resonance, and reporter gene assays, we discovered that human Cerberus

116 Applying in vitro luciferase reporter gene assays, we show that expression of short A

117 lead risk variant region were analyzed with reporter gene assays.

118 47 like ETS transcription factor 5 (ELF5) in reporter gene assays.

119 endent interaction with miRNAs, as proven by reporter gene assays.

120 anscript, which was further confirmed in NMD reporter gene assays.

121 oinformatic target prediction and luciferase reporter gene assays.

122 Ps, using GAL4-UAS-based in vitro luciferase reporter gene assays.

123 However, reporter-gene assays, impedance-based assays with a sele

124 We also present a firefly luciferase (FLuc) reporter gene based molecular biosensor (ARE-FLuc) to me

125 onse and Stx1/2 production, we constructed a reporter gene based test system that allows for the time

126 However, existing MRI reporter genes based on metalloproteins or chemical exch

127 Here, we use a reporter gene-based screen in Saccharomyces cerevisiae f

128 for DNA double-strand breaks on the GFP-Pem1 reporter gene by homologous recombination, the persisten

129 The activation of reporter genes by artificial fusion of Mediator subunits

130 We find that expression noise and mean of a reporter gene can be tuned independently by CRISPRar.

131 R-loop formation by synonymous changes in a reporter gene can lower mutation rate by >80%.

132 These foreign reporter genes can be used as valid surrogates to track

133 Nevertheless, a reporter gene cassette inserted into the vector flanked

134 An independent reporter gene cassette located in an intergenic region r

135 no ability to activate transcription of the reporter gene CDKN1A, and in situ subcellular fractionat

136 ctionation approach, a downscaled luciferase reporter gene cell-based anti-AR-CALUX assay and LC-HRMS

137 es the translational ability of a luciferase reporter gene coding sequence when it is preceded by mul

138 Reporter genes confirmed that the host responses were du

139 While employing reporter genes constructed with inducible promoters and

140 y has grown to over 23 000 records of tested reporter gene constructs and 2200 tested transcription f

141 rongly than +1164G and that Hap-I-containing reporter gene constructs have increased basal and HNF3-

142 Furthermore, the expression of a luciferase reporter gene containing the 3'-UTR of CYP2C9 and the en

143 e performed chromatin immunoprecipitation on reporter genes containing these elements in different em

144 position, number and sequence of introns in reporter genes controlled by the hsp-90 promoter.

145 opoiesis and by doing so validate the use of reporter gene-coupled enhancers as probes to gain insigh

146 show that human EC cells efficiently express reporter genes delivered by vectors based on human immun

147 long-term knockdown of a corneal epithelial reporter gene, demonstrating gene disruption via NHEJ in

148 hers to select a fluorescent or a luciferase reporter gene, depending on the type of study.

149 Y5Y cells that stably expressed a luciferase reporter gene driven by the BACE1 (beta-site amyloid pre

150 AGL1 harboring the beta-glucuronidase (GUS) reporter gene driven by the cauliflower mosaic virus 35S

151 ing for altered expression of the luciferase reporter gene driven by the promoter from the heat-induc

152 iscosoma sp. red fluorescent protein (DsRed) reporter genes driven by either CaMV35S or intron-interr

153 Previous work shows that expression of reporter genes driven by testis-specific promoters is co

154 Induction of reporter genes, driven by truncated Ifit1 promoters, ide

155 or activate an antioxidant response element reporter gene due to the absence of a Keap1-binding moti

156 rced to select a fluorescent or a luciferase reporter gene due to the restricted capacity of the infl

157 of silencing of a dominant selectable kanMX reporter gene embedded within fission yeast centromeric

158 H2.35 cells expressing an estrogen receptor reporter gene, estrogen receptor 1, and/or PGC1A/B.

159 We also show that linked reporter genes exhibit coordinated bursting profiles whe

160 eport comprehensive ChIP-Seq, transgenic and reporter gene experimental data that have allowed us to

161 An SYNV minireplicon reporter gene expression assay showed that the M protein

162 nsertion locations likely contributed to the reporter gene expression being exquisitely sensitive BMS

163 wild-type AAV2 (wtAAV2) replication but also reporter gene expression from both single-stranded and d

164 hat the ICP4 locus cassette permitted robust reporter gene expression in a diversity of neurons follo

165 , we used quantitative PCR and also analyzed reporter gene expression in a mouse line carrying a cons

166 riants previously proposed as causal lead to reporter gene expression in a pattern consistent with th

167 Moreover, gar enhancers drive reporter gene expression in both the wrist and digits of

168 urther, we show that the Vcan enhancer drove reporter gene expression in endocardial lineages in a TB

169 promoter region, -505 to +382 bp, activated reporter gene expression in mouse retina in vivo.

170 15 DHSs from pha-4, icl-1, and ceh-13 drove reporter gene expression in transgenic C. elegans Overal

171 ion factors, and is necessary for activating reporter gene expression in vitro and in vivo.

172 ion, and non-endogenous (ectopic) aspects of reporter gene expression is under development and expect

173 The reporter gene expression level was confirmed via immunob

174 A dramatic drop of reporter gene expression of the oxytocin exon 1-intron-e

175 interrogation of genetic elements that alter reporter gene expression readout.

176 Further reporter gene expression suggested that variant -842C-66

177 WT1 regulates reporter gene expression through interaction with 3' UTR

178 We have used imaging of reporter gene expression to track transcription in livin

179 when comparing single modified mRNAs) higher reporter gene expression upon transfection into cell lin

180 ith human PBMCs or hematopoietic stem cells, reporter gene expression was predominantly detected in l

181 We uncover a H1 enhancer sufficient to drive reporter gene expression within the crNCCs of the distal

182 Here, using biochemical assays, reporter gene expression, and confocal fluorescence micr

183 d assays, including SMAD-mediated luciferase reporter gene expression, and differentiation of a multi

184 to efficiently suppress NF-kappaB-dependent reporter gene expression, in contrast with the vaccine s

185 otides, using their inferred contribution to reporter gene expression.

186 and prostate cancer cell lines, and elicited reporter-gene expression following intra-tumoural and in

187 onse element (GRE) and exclusively increased reporter-gene expression.

188 ate mapping with a thymidine kinase (sr39tk) reporter gene for cell detection by positron emission to

189 Using a reporter gene for eilA activation, we defined conditions

190 gene has previously been translated as a PET reporter gene for imaging of T-cell trafficking in patie

191 Plasmids were generated carrying reporter genes for fluorescence, bioluminescence imaging

192 n alternative class of sensitive, metal-free reporter genes for non-invasive imaging.

193 ulated, we first developed plasmid-based UAS reporter genes for the Dscam variable exon 4 cluster and

194 ) and a bisegmented TCRV expressing a single reporter gene from a bicistronic viral mRNA (rTCRV/GFP).

195 ding the transcription start site (TSS) of a reporter gene fusion in Arabidopsis thaliana The intron

196 Using reporter gene fusions and transcriptomics, here we repor

197 ransgenic plants expressing either ProCgNIN::reporter gene fusions or CgNIN RNAi constructs.

198 rapeseed lines expressing Bn-FAE1.1 promoter:reporter gene fusions revealed a strong expression in th

199 Expression of the reporter genes (GFP and YFP) was also confirmed using re

200 We linked this to the beta-glucuronidase reporter gene gusA.

201 ical exchange saturation transfer (CEST) MRI reporter gene has previously been developed and used to

202 Reporter gene imaging allows for non-invasive monitoring

203 nd of the review is whether the "promise" of reporter gene imaging has been realized.

204 l manganese-enhanced MRI and bioluminescence reporter gene imaging were applied to follow myocardial

205 hylline-dependent down-regulation of the GFP reporter gene in a dose- and time-dependent manner.

206 an enhanced green fluorescent protein (EGFP) reporter gene in a panel of 12 organs after IP injection

207 ity after large-scale random insertions of a reporter gene in a population of Escherichia coli bacter

208 Equation)-based model of the expression of a reporter gene in an all E. coli TXTL that we apply to a

209 vate the expression of a pathogen-responsive reporter gene in Arabidopsis (Arabidopsis thaliana).

210 of the pathogen-responsive CaBP22(-333)::GUS reporter gene in Arabidopsis thaliana (Arabidopsis), 33

211 , we modulate the gene expression noise of a reporter gene in Escherichia coli by incorporating CRISP

212 SPFMV gene products coagroinfiltrated with a reporter gene in Nicotiana benthamiana revealed that P1N

213 ogy based on the conditional activation of a reporter gene in response to sequence errors occurring a

214 could drive M-cell-specific expression of a reporter gene in rice.

215 b promoter supports enhanced expression of a reporter gene in sphk2(MZ) embryos compared to wildtype

216 This strategy prevents expression of the reporter gene in the transfected cells but permits its e

217 ion of a downstream beta-glucuronidase (GUS) reporter gene in tobacco leaves.

218 genetic switches to activate and inactivate reporter genes in a predetermined order.

219 mice, ~30% of GHRH neurons coexpressed both reporter genes in adult females, but not in males.

220 chromatin structure and loss of silencing of reporter genes in constitutive heterochromatin regions.

221 e to bind DNA in vitro and transactivate SOX reporter genes in cultured cells.

222 ng for targeted integration of human imaging reporter genes in human embryonic stem cells for long-te

223 s (ULG8), which we have leveraged to express reporter genes in mature male and female gametocytes.

224 Expression of these reporter genes in transient assays as well as in T1 stab

225 expression of intronless and intron-bearing reporter genes in vitro.

226 sues, and analyzed the expression of several reporter genes, including ProHY5:HY5-GFP and Pro35S:CFP-

227 arted by the 5 UTR can be transferred onto a reporter gene, indicative that the 5 UTR can solely driv

228 TALE-reconstitution and can be monitored by reporter gene induction.

229 s contain an antisense-oriented promoter and reporter gene interrupted by a sense-oriented intron fro

230 We succeeded in transferring a GFP reporter gene into adult hematopoietic stem cells in viv

231 CoV-2 (icSARS-CoV-2-mNG) by introducing this reporter gene into ORF7 of the viral genome.

232 We show that insertion of a luciferase reporter gene into the endogenous tyrosine hydroxylase (

233 nt rotaviruses that express FPs by inserting reporter genes into the NSP1 ORF of genome segment 5.

234 to induce stable expression of human imaging reporter genes into the safe-harbor locus adeno-associat

235 tandem duplication of an unrelated synthetic reporter gene is overactive (2.3- to 5.1-fold) at all si

236 we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the

237 Promotor-driven reporter gene labeling of the cells under investigation

238 Surprisingly, using a reporter gene linked to activation of the MAPK substrate

239 n the 5'-UTR promotes the translation of the reporter gene mRNA.

240 We utilized a reporter gene (mURA3) integrated adjacent to the leftmos

241 orter IAV (BIRFLU) that stably expresses two reporter genes (one fluorescent and one luciferase) to t

242 We created a library of circuits with two reporter genes, one constitutive and the other inducible

243 llators using a mouse model expressing three reporter genes: one labeling alpha cells, one specific f

244 ng that promoter and derivatives between two reporter genes oriented head to head, we show that the T

245 with three plasmids carrying a fluorescence reporter gene (pEF-GFP with the EF1 alpha promoter, pUB-

246 ail target enhancers were attached to an MS2 reporter gene, permitting detection of nascent transcrip

247 ges were monitored using a novel fluorescent reporter gene, pMitoTimer, that allows assessment of mit

248 enhancers positioned downstream of synthetic reporter genes produce transcriptional bursts with simil

249 reading frame (ORF) and accumulation of the reporter gene product in tobacco chloroplasts encoded in

250 (a trisegmented form of TCRV expressing two reporter genes [r3TCRV]) and a bisegmented TCRV expressi

251 hieve a 110-fold increase in expression of a reporter gene relative to non-replicating controls.

252 A comparison of different reporter gene-reporter probe systems for imaging of T ce

253 venous injection of rAAV2/9 carrying an eGFP-reporter gene results in binaural transduction of inner

254 Reporter gene selection is vital to a sensor performance

255 ver, the highly repetitive nature of the LRP reporter gene sequence leads to DNA recombination events

256 The KAK promoter fused with a reporter gene showed activity in the vascular cambium, p

257 ibited serum response factor (SRF)-dependent reporter gene (SRE-LUC) activity and mRNA expression of

258 ed with a positron emission tomography (PET) reporter gene strategy.

259 diated by the cAMP response element (CRE) in reporter gene studies (10-fold vs. control; n = 4 transf

260 n vitro gel shifts and by in vivo functional reporter gene studies.

261 At the cellular level, pCkx3::YFP reporter-gene studies revealed that the Ckx3 promoter is

262 xpression were identified using a transgenic reporter gene system subdividing the B cell progenitors

263 Here we report the development of a PET reporter gene system using the PKM2 gene with its associ

264 Using a reporter gene system, we showed that Msi1 competes with

265 scriptional activity-which is now known as a reporter gene system.

266 Therefore, we focused on human reporter gene systems that have the potential for transl

267 Tagging donor sequences with reporter genes that can be subsequently excised improves

268 moted T lymphocyte-specific transcription of reporter genes throughout T cell development; however, i

269 Here the authors use a mitochondrial reporter gene to demonstrate the occurrence of mitophagy

270 ia coli CRE isolate as well as a fluorescent reporter gene to easily monitor plasmid maintenance.

271 s the transcription status of the downstream reporter gene to provide a rapid and facile readout of t

272 es expressing FPs, one relying on fusing the reporter gene to the NSP3 ORF of genome segment 7.

273 We used genome analogues expressing reporter genes to assess the abilities of Phlebovirus nu

274 and advances in the introduction and use of reporter genes to enable longitudinal imaging.

275 RNA) and GFP plasmid DNA (pDNA) were used as reporter genes to evaluate NP-mediated gene transfection

276 uire molecular oxygen; therefore, the use of reporter genes to study molecular mechanisms in anaerobi

277 -retinoid X receptor (RXR) complex and drove reporter gene transcription in response to 1,25-dihydrox

278 the Smc5/6 complex enhances extrachromosomal reporter gene transcription in the absence of HBx, resto

279 therapies and 2) is correlated with optical reporter gene transduction of the brain.

280 ion at colocalized sites were established by reporter gene transfection assays.

281 rocarbon chains showed the highest amount of reporter gene transfection in the retina.

282 The reporter gene used in the study is the green fluorescent

283 es placed at the 3' untranslated region of a reporter gene using a lentiviral delivery system with re

284 nd herbicide (aad1) resistance, and a phiyfp reporter gene using a single ZMUbi1 bidirectional promot

285 We also discuss employing NIS as a reporter gene using viral vectors and stem cells in imag

286 Gene expression was not blocked when the reporter gene was delivered by microbombardment.

287 The PKM2 reporter gene was delivered to the brains of mice by ade

288 Fluorescence signal from the reporter gene was detected a few hours after transfectio

289 tor expressing the green fluorescent protein reporter gene was evaluated in the cat.

290 s relying on green fluorescent protein (GFP) reporter genes, we found that targeted mutagenesis by CR

291 Using a series of reporter genes, we found that the in vivo l-Trp sensitiv

292 Using a mouse model with two reporter genes, we observed that, while GHRH(tdTom) neur

293 , BIRFLU effectively infected mice, and both reporter genes were detected using in vivo imaging syste

294 fK binding sites are sufficient to repress a reporter gene when cloned in genome-integrated lentivira

295 nalized virus remained capable of expressing reporter genes while viral replication was blocked.

296 We describe reporter gene whole cell-based biosensor systems based o

297 transfected with rdLRP demonstrated a stable reporter gene with a single distinct band corresponding

298 ly 14,000 E. coli strains, each expressing a reporter gene with a unique 5' architecture.

299 on of specific cell types by combining a PET reporter gene with Cre-dependent activation that can be

300 s of real-time PCR for the neuronal activity reporter gene zif268.