ライフサイエンスコーパス: resolvase

1 se, suggesting it acts as a post-replicative resolvase.

2 nformation of the DNA junction when bound by resolvase.

3 nctions (HJs), identifying SLX1-SLX4 as a HJ resolvase.

4 es x res recombination promoted by wild-type resolvase.

5 proach for identifying a eukaryotic Holliday resolvase.

6 and regulation in recombination by wild-type resolvase.

7 sed to be an example of a eukaryotic nuclear resolvase.

8 of double Holliday junctions (HJs) by an HJ resolvase.

9 donuclease Mus81-Eme1 is a Holliday junction resolvase.

10 ed that it functions as a Holliday structure resolvase.

11 for the broad substrate specificity of phage resolvase.

12 ke structures in cells lacking Mus81-Eme1 HJ resolvase.

13 ted tet gene flanked by targets for the TnpR resolvase.

14 avages characteristic of a Holliday junction resolvase.

15 interface that abolish activity of wild-type resolvase.

16 1 from humans and fission yeast encodes a HJ resolvase.

17 expression of a bacterial Holliday junction resolvase.

18 tes DNA repair in strains lacking the RuvABC resolvase.

19 obstacle may interfere with the binding of a resolvase.

20 expression of a bacterial Holliday junction resolvase.

21 s evaluated from the degree of inhibition of resolvase.

22 are subunits of a nuclear Holliday junction resolvase.

23 sis to investigate such residues in the RusA resolvase.

24 binding and catalysis by a Holliday junction resolvase.

25 ers, required the meiosis-specific MutLgamma resolvase.

26 ystal structure of the canarypox virus (CPV) resolvase.

27 n established as a major cellular quadruplex resolvase.

28 dimers in the synaptic complex of gammadelta resolvase.

29 t to a regulatory sequence recognized by Tn3 resolvase.

30 ing protein Brc1 and Mus81 Holliday junction resolvase.

31 SOS induction, and RuvABC Holliday-junction resolvase.

32 /RecBCD is compared to that of the RecU/RuvC resolvases.

33 fferent functions of the viral and bacterial resolvases.

34 transposases and RuvC-like Holliday junction resolvases.

35 tructure-selective endonucleases known as HJ resolvases.

36 opological selectivity as Tn3 and gammadelta resolvases.

37 new type of substrate for Holliday junction resolvases.

38 that encode putative Holliday junction (HJ) resolvases.

39 pite a lack of sequence homology between the resolvases.

40 mmetry, a feature more akin to cellular RuvC resolvases.

41 sing shared by prokaryotic and eukaryotic HJ resolvases.

42 We also discovered that G4 Resolvase 1 (G4R1) enhanced the Gluc expression mediated

43 se properties to GQ targeting ligands and G4 resolvase 1 (G4R1) protein binding.

44 gene product of DHX36, naming the enzyme G4 Resolvase 1 (G4R1).

45 associated with AU rich elements) (RHAU) (G4 resolvase 1), Bloom helicase (BLM), and Werner helicase

46 to the putative resolution site for the ParA resolvase, a potential hot spot for those transposons.

47 Resolvase acting on these sites cleaves the intact stran

48 This resolvase, activated for recombination by mutations, for

49 hanism of strand exchange and the process of resolvase activation are discussed.

50 This mechanism stands in contrast to classic resolvase activities that use a structure-specific endon

51 Curbing resolvase activity and establishing a hierarchy of resol

52 ain of Ydc2 resulted in the complete loss of resolvase activity and impaired significantly the bindin

53 helicase shown to have G-quadruplex (G4)-RNA resolvase activity and the major source of G4-DNA resolv

54 on analyses, we found that Holliday junction resolvase activity associated tightly and co-eluted with

55 preferences for Mg(2+) versus Mn(2+) Optimal resolvase activity is maintained with 5 mum Mn(2+) and 1

56 provide evidence that the Holliday junction resolvase activity of Ydc2 is required for mtDNA transmi

57 Their resolvase activity shares the characteristics of the Esc

58 etramolecular G4-DNA into single strands (G4-resolvase activity) has been observed only in recombinan

59 liday junctions, but does not show canonical resolvase activity, implying that the endonuclease incis

60 h Holliday junctions, but shows no canonical resolvase activity.

61 le for the majority of tetramolecular G4-DNA resolvase activity.

62 es RAD51C or XRCC3 have reduced levels of HJ resolvase activity.

63 ion of gene expression that relies on its G4 resolvase activity.

64 vase activity and the major source of G4-DNA resolvase activity.

65 erculosis so that it can act as a dimeric HJ resolvase analogous to that of RuvC.

66 has been proposed to be a Holliday junction resolvase and has now been found to be responsible for n

67 These results demonstrate that unique resolvase and invertase derivatives can be developed to

68 network of amino acid residues that mediate resolvase and invertase DNA sequence specificity.

69 mutagenesis revealed the molecular basis of resolvase and invertase DNA sequence specificity.

70 extensively redesigned and highly convergent resolvase and invertase populations in the context of en

71 al intriguing structural differences between resolvase and RuvC, and a model of the CPV resolvase.Hol

72 ike a canonical, RuvC-like Holliday junction resolvase and support a novel model in which Mlh1-Mlh3 i

73 hat of a presynaptic complex between dimeric resolvase and the cleavage site DNA.

74 using the resII-resIII synapse formed by Tn3 resolvase and the LER synapse formed by phage Mu transpo

75 ne dependent on the RuvABC Holliday junction resolvase and the other on RecG helicase.

76 that a specific synaptic structure formed by resolvase and the res accessory sequences permits Cre to

77 ic synaptic complex containing a tetramer of resolvase and two crossover sites.

78 ecombinase family, which includes transposon resolvases and DNA invertases, in that they utilize two

79 at its Atu2523 gene encodes a protelomerase (resolvase) and that the purified enzyme can generate the

80 ase, retroviral integrase, Holliday junction resolvase, and RISC nuclease Argonaute.

81 irus, bacterial, and fungal mitochondrial HJ resolvases are consistent with their predicted evolution

82 Junction resolvases are generally small, homodimeric endonuclease

83 The model also explains why the poxvirus resolvases are more promiscuous than RuvC, cleaving a va

84 Holliday junction (HJ) resolvases are necessary for the processing of persisten

85 Holliday junction (HJ) resolvases are structure-specific endonucleases that cle

86 ally, we identify GEN-1, a Holliday junction resolvase, as an evolutionarily conserved WDR-23 substra

87 Furthermore, the dimeric form of the resolvase associated with HJ DNA, presumably facilitatin

88 Like all known eukaryotic resolvases, AtGEN1 and Arabidopsis single-strand DNA end

89 f resolvase-mediated cleavage and effects on resolvase binding and synapsis, providing insight into t

90 ve D70N variant of the Escherichia coli RusA resolvase bound to a duplex DNA substrate that reveals c

91 nvestigated the stability of synapses of Tn3 resolvase-bound res recombination sites, in plasmids con

92 we propose that pairing interactions between resolvase-bound res sites are in a state of rapid flux,

93 nsight into how a Holliday junction-specific resolvase can evolve into a debranching endonuclease tai

94 scopy that purified T4 gp49 endonuclease VII resolvase can release DNA compression in vitro in prohea

95 gment, and pht operon, encoding a transposon resolvase, catabolism of protocatechuate (3,4-dihydroxyb

96 The serine recombinase Tn3 resolvase catalyses recombination between two 114 bp res

97 ssover sites are far apart, separated by the resolvase catalytic domains bound to them.

98 e therefore the principal reasons underlying resolvase cell cycle regulation.

99 n vector containing a codon optimized serine resolvase CinH recombinase (CinH) and its recognition si

100 Here, we report an assay for resolvase cleavage activity based on fluorescence polari

101 deletion by blocking Holliday junction (HJ) resolvase cleavage activity, but whether TRF2 also modul

102 A viral resolvase cleaves DNA four-way junctions extruded at the

103 A22 resolvase cleaves Y-junctions, single-stranded DNA flaps

104 he RAD51C-XRCC3-associated Holliday junction resolvase complex associates with crossovers and may pla

105 characterized, but an atomic structure of a resolvase complex with a Holliday junction remained elus

106 sequently undermined upon addition of the HJ resolvase complex, RuvABC, which resulted in significant

107 luding the RecG helicase and RuvABC junction resolvase complex.

108 structure of a eukaryotic Holliday junction resolvase confirms a distant evolutionary relationship t

109 of the site-specific recombinase gammadelta resolvase covalently linked through Ser10 to two cleaved

110 d demonstrate that it promotes DNA repair in resolvase-deficient ruv mutants of E. coli.

111 Transcription of the fusion leads to resolvase-dependent excision of a sacB-containing casset

112 Viral and bacterial Holliday junction resolvases differ in specificity with the former typical

113 Each res binds a resolvase dimer at site I, where strand exchange takes p

114 tive complex, the catalytic domains from two resolvase dimers form a central core, while the DNA bind

115 s an architectural role that is taken by two resolvase dimers in models of the Tn3/gammadelta synapse

116 nge when two res recombination sites and six resolvase dimers interact to form a synapse.

117 embled exclusively by R interactions between resolvase dimers, except for the one special dimer-dimer

118 ch of which contains binding sites for three resolvase dimers.

119 stly, we describe possible roles for the A22 resolvase DNA-branch nuclease activity in DNA replicatio

120 alent metals dictate the conformation of FPV resolvase-DNA complexes and subsequent DNA cleavage.

121 The covalent resolvase-DNA linkages in the natural reaction intermedi

122 sisting of an OB-fold and a holiday junction resolvase domain.

123 role of the vaccinia virus Holliday junction resolvase during an infection, we constructed two recomb

124 ogous to that exhibited by the prototypic HJ resolvase E. coli RuvC.

125 ce charge potential of the Holliday junction resolvases endo VII, RuvC, Ydc2, Hjc and RecU, despite h

126 , topoisomerase action and Holliday junction resolvases, ensure that all SCIs are removed before they

127 ions, which are cleaved by the virus-encoded resolvase enzyme to form unit-length genomes.

128 Resolvase enzymes that cleave DNA four-way (Holliday) ju

129 m similar to that shown by the prototypic HJ resolvase, Escherichia coli RuvC protein, but it is uncl

130 insights into how archaeal Holliday junction resolvases evolved to incise 5' flap substrates and how

131 serine-integrase, a member of the transposon-resolvase family of site-specific recombinases.

132 utants of the serine recombinase, gammadelta resolvase, form a simplified recombinogenic synaptic com

133 Fowlpox resolvase formed complexes with a variety of branched DN

134 Fowlpox virus resolvase (Fpr) is an endonuclease that cleaves a broad

135 e determined the crystal structure of a RuvC resolvase from bacteriophage bIL67 to help identify feat

136 y that exploits a previously uncharacterized resolvase from Bartonella bacilliformis ("Bart").

137 vC is a well-characterized Holliday junction resolvase from E. coli.

138 invertase Gin from bacteriophage Mu and Tn3 resolvase from Escherichia coli.

139 (also known as SpCce1), a Holliday junction resolvase from the fission yeast Schizosaccharomyces pom

140 d Holliday junction cleaving (Hjc) family of resolvases from the moderately thermophilic archaeon Met

141 Resolvases from the serine recombinase family assemble a

142 he site-specific DNA recombinase, gammadelta resolvase, from Escherichia coli catalyzes recombination

143 Bacterial RuvC, a prototypical HJ resolvase, functions as homodimer and nicks DNA strands

144 we present the crystal structure of human HJ resolvase GEN1 complexed with DNA at 3.0 A resolution.

145 The human HJ resolvase GEN1 is a member of the XPG/Rad2 family of 5'-

146 The human Holliday junction resolvase, GEN1, and its yeast orthologue, Yen1, were in

147 a defective H-19B prophage encoding the TnpR resolvase gene downstream of the phage PR promoter and a

148 The resolvase gene is regulated by a promoter activated upon

149 transcriptional fusions of genomic DNA to a resolvase gene was ingested by five healthy adult volunt

150 dal gene surrounded by two res1 sites and a resolvase gene, tnpR, which acts at the res1 sites.

151 Knockout of resolvase genes GEN1 and SEND1, individually or together

152 he identification of a eukaryotic nuclear HJ resolvase has been elusive.

153 mutant; and (3) expression of a bacterial HJ resolvase has no suppressive effect on mus81 meiotic phe

154 Previous studies of the vaccinia virus resolvase have been problematic due to poor protein solu

155 Although HJ resolvases have been identified in all domains of life,

156 Several Holliday junction resolvases have since been characterized, but an atomic

157 Enzymes that cleave HJs, called HJ resolvases, have been identified in all domains of life

158 However, the D7N active-site mutant resolvase held the arms in a more planar arrangement in

159 isPINA), encoded by the gene adjacent to the resolvase Hjc coding gene.

160 annotated to be a putative Holliday junction resolvase (HJR).

161 n resolvase and RuvC, and a model of the CPV resolvase.Holliday junction complex provides insights in

162 that Rap can function as a Holliday junction resolvase in addition to eliminating other branched stru

163 hat are reminiscent of the classical RuvC HJ resolvase in bacteria.

164 Here we report that A22 resolvase in fact has a much wider substrate specificity

165 EAH-box helicase 36 (DHX36), the dominant G4 resolvase in human cells.

166 The absence of a homologous resolvase in mammalian cells makes these microbial enzym

167 that Mus81/Mms4 is not the major meiotic HJ resolvase in S. cerevisiae: (1) MUS81/MMS4 is required t

168 les for the vaccinia virus Holliday junction resolvase in the replication and processing of viral DNA

169 The catalytic domains of resolvase in the unliganded structure are arranged asymm

170 interest to further characterize a poxvirus resolvase in view of the low sequence similarity with Ru

171 Significantly, overexpression of the resolvase in wild-type cells partly mimics the loss of v

172 studies reveal potential roles for these HJ resolvases in repair after DNA damage and during meiosis

173 ites for site-specific recombination by Tn21 resolvase, in buffers that contained either EDTA or CaCl

174 ites for site-specific recombination by Tn21 resolvase; inhibition of recombination would indicate th

175 The site-specific recombinase Tn3 resolvase initiates DNA strand exchange when two res rec

176 rine recombinase catalytic mechanism and how resolvase interacts with the substrate DNA.

177 striction endonucleases, DNA-repair enzymes, resolvases, intron splicing factors and transcription fa

178 vel group of phage integrases related to the resolvase/invertase enzymes.

179 activated catalytic domain derived from the resolvase/invertase family of serine recombinases and a

180 Hyperactivated variants of the resolvase/invertase family of serine recombinases functi

181 activated catalytic domains derived from the resolvase/invertase family of serine recombinases fused

182 egrase family and the serine recombinases or resolvase/invertase family.

183 In contrast to the resolvase/invertase recombination systems--where there a

184 nctionally versatile and include integrases, resolvases, invertases and transposases.

185 us F16L and prokaryotic serine site-specific resolvase-invertases.

186 e phiC31 encodes an integrase related to the resolvase/invertases and is evolutionarily and mechanist

187 The archetypal resolvase/invertases are highly regulated, only affect r

188 different group, are similar in size to the resolvase/invertases but have the DNA binding domain N-t

189 The well studied serine recombinases, the resolvase/invertases, bring two recombination sites toge

190 e three structural groups represented by the resolvase/invertases, the large serine recombinases and

191 longer C-terminal domains compared with the resolvase/invertases.

192 and the resT gene, which encodes a telomere resolvase involved in resolution of the replicated telom

193 mbinational repair, but since a classic RecG resolvase is absent from H. pylori, deployment of the Re

194 to cell division, the Yen1 Holliday junction resolvase is activated at anaphase.

195 on is required for viral replication, so the resolvase is an attractive target for small molecule inh

196 Catalysis of DNA recombination by Tn3 resolvase is conditional on prior formation of a synapse

197 CPV resolvase is dimer of RNase H superfamily domains relate

198 specificity: the branch migration-associated resolvase is highly specific for Holliday junctions, whe

199 The virus-encoded Holliday junction resolvase is required to process concatemers into unit-l

200 The ResT telomere resolvase is responsible for maintaining the hairpin tel

201 r nuclease, the Mus81-Eme1 Holliday junction resolvase, is required to generate crossovers accompanyi

202 tion, our study revealed a Holliday junction resolvase-like activity in the liver that cleaved T-shap

203 ins, a third OB fold and a Holliday junction resolvase-like domain.

204 t protein and cleaved in a Holliday junction resolvase-like reaction.

205 a tetrameric synaptic complex of gammadelta resolvase linked to two cleaved DNA strands had suggeste

206 on or Holliday junction resolution by a host resolvase may contribute to integration in vivo.

207 To provide a platform for understanding resolvase mechanism and designing inhibitors, we have de

208 iesters result in abolition or inhibition of resolvase-mediated cleavage and effects on resolvase bin

209 However, after infection of these cells, resolvase-mediated excision of the dal gene resulted in

210 oxP sites in several distinct ways, and that resolvase-mediated intertwining of the accessory sequenc

211 een subunits that is essential for wild-type resolvase-mediated recombination.

212 Previously we reported that Tn3 resolvase-mediated synapsis of the accessory binding sit

213 We find that the two resolvase metal binding sites have different preferences

214 ynapse is assembled by sequential binding of resolvase monomers to site I followed by interaction of

215 'Hyperactive' resolvase mutants, that catalyse strand exchange at site

216 ontaining mixtures of wild-type Tn3 and Bart resolvase NTD dimers are recombination-defective, but ac

217 requires a specific "R" interface involving resolvase NTDs at all three dimer-binding sites in res.

218 eriophage S-PM2, which is similar to the DNA resolvase of bacteriophage T4 and is encoded adjacent to

219 factors on the activity and structure of the resolvase of fowlpox virus, which provides a tractable m

220 process HJs in a manner analogous to the HJ resolvases of phages, archaea, and bacteria.

221 que topology being conferred by the external resolvase or LER synapse.

222 by site-specific recombinases or by junction resolvases or helicases.

223 The serine recombinase gamma delta resolvase performs site-specific recombination in an ela

224 Once made, the wild-type resolvase persists in cells for time periods greater tha

225 e WRN protein or by expressing the bacterial resolvase protein RusA.

226 rejoining enzyme known as a hairpin telomere resolvase (protelomerase).

227 that evolutionary loss of the H. pylori RecG resolvase provides an "antirepair" pathway allowing for

228 can be restored by replacing patches of Tn3 resolvase R interface residues with Bart residues, or vi

229 Tn3 and Bart resolvases recognize different DNA motifs, via diverged

230 bined substrates derived from the native Tn3 resolvase recombination site.

231 l gene tightly regulated by an actA-promoted resolvase recombination system.

232 The eukaryotic Holliday junction resolvases represent a new subclass of the Rad2/XPG fami

233 essential functions, including the telomere resolvase, ResT, and hence cannot be displaced.

234 Expression of resolvase results in excision of tet, restoring a functi

235 n recently identified as a Holliday junction resolvase, results in increased sensitivity of the cells

236 s, bacterial transposases, Holliday junction resolvases, retroviral integrases and many other enzymes

237 Likewise, expression of the bacterial HJ resolvase RusA partially rescues the defects of nse6Delt

238 y suppressed by overexpressing the bacterial resolvase RusA.

239 ls could be cleaved by the Holliday junction resolvases RusA and RuvC.

240 By introducing an E. coli resolvase (RusA) into H. pylori, the repair and recombin

241 ision repair uvrABC, recombinases recBCD and resolvases ruvABC) were not induced.

242 tructural homology with the Escherichia coli resolvase RuvC but Ydc2 contains a small triple helical

243 Mus81-Eme1, is as good as the archetypal HJ resolvase RuvC in single turnover kinetic analysis.

244 Type II R-M systems, the nuclease nucT and resolvase ruvC reduced integration length whereas the he

245 , showing that, like the Escherichia coli HJ resolvase RuvC, it binds specifically to HJs and resolve

246 wn by the Escherichia coli Holliday junction resolvase RuvC.

247 The identity of the resolvase(s) that functions in vivo has yet to be establ

248 A short half-life resolvase showed that barriers appeared and disappeared

249 The resolvase Sin regulates DNA strand exchange by assemblin

250 is thaliana has two putative homologs of the resolvase (structure-specific endonuclease): GEN1/Yen1.

251 Comparisons of the four known tetrameric resolvase structures show that the subunits interact thr

252 half-site that is not covalently linked to a resolvase subunit dissociates rapidly from the synapse,

253 an elaborate synaptic complex containing 12 resolvase subunits and two 114-base pair res sites.

254 prior formation of a synapse, comprising 12 resolvase subunits and two recombination sites (res).

255 Resolvase subunits in the complex contact their neighbor

256 would require a substantial rearrangement of resolvase subunits or domains.

257 ssembling the topologically well defined Tn3 resolvase synapse, it is possible to determine whether t

258 re of the regulatory site synapse in the Sin resolvase system.

259 Cleavage of the cruciform by the junction resolvase T4 endonuclease VII is independent of PARP-1,

260 Phage-encoded resolvase T7 endonuclease I is a structure-specific endo

261 e formation of a stable synapse comprising a resolvase tetramer and two copies of site I.

262 n a synapse of two sites, held together by a resolvase tetramer; cleavage at one site stimulates clea

263 Stable resolvase tetramers were not detected in the absence of

264 oduction of modified substrate cassettes for resolvase that can be positively and negatively selected

265 iochemical studies show that SLX-MUS is a HJ resolvase that coordinates the active sites of two disti

266 results show that DDX5 is a highly active G4-resolvase that does not require a single-stranded overha

267 resolution, forms a synaptic intermediate of resolvase that is covalently linked to two cleaved DNAs,

268 y employing a portal-bound Holliday junction resolvase that trims and releases these DNA roadblocks t

269 of the site-specific recombinase, gammadelta resolvase, that form activated tetramers have been deter

270 time assays, showing that, as with vaccinia resolvase, the fowlpox enzyme could cleave a wide array

271 ng up to six res recombination sites for Tn3 resolvase to analyse looping interactions (synapsis) in

272 No mutations strongly inhibited binding of resolvase to site I, but several caused conspicuous chan

273 eed RecA recombinase and a Holliday junction resolvase to survive rapid growth, but SOS induction, al

274 utations at the interface that either enable resolvase to synapse two copies of site I or inhibit syn

275 rgeted an Escherichia coli Holliday junction resolvase to the nuclei of fission yeast recombination-d

276 used a reporter construct, the tnpR-encoded resolvase, to assess terminators.

277 ith linear DNA; the reduction is reversed by resolvase treatment.

278 These findings provide a tractable poxvirus resolvase usable for the development of small molecule i

279 dues in the catalytic domain of an activated resolvase variant.

280 We have analysed the in vitro properties of resolvase variants with 'activating' mutations, which ca

281 When resolvase was added to a SfiI binding reaction in EDTA f

282 Cleavage by resolvase was also tightly coupled at symmetrical positi

283 ells infected with vA22-HA revealed that the resolvase was expressed after the onset of DNA replicati

284 The putative vaccinia virus resolvase was expressed as a recombinant protein, affini

285 We found that fowlpox virus resolvase was much more tractable.

286 east CCE1 gene, encoding a Holliday junction resolvase, was introduced into cells carrying partially

287 nique active site structure observed for CPV resolvase, we have carried out a series of experiments t

288 ages catalysed by the serine recombinase Tn3 resolvase, we made modified recombination sites with a s

289 joining by the site-specific recombinase Tn3 resolvase, we mutated conserved polar or charged residue

290 Vaccinia encodes an enzyme, A22 resolvase, which is known to be active on four-stranded

291 in the RuvC family of Holliday junction (HJ) resolvases, which have a key role in homologous recombin

292 li radiation and UV sensitive C paradigm for resolvases, which involves resolving HJs by symmetricall

293 precleavage synaptic tetramer of gammadelta resolvase, whose structure is not known, may be formed b

294 r than the 150 domains detected with the Tn3 resolvase, wild-type cells measured over a 10 min time s

295 cible transcription, we constructed a set of resolvases with cellular half-lives ranging from less th

296 Holliday junction resolvases with distinct properties have been characteri

297 d properties of eukaryotic Holliday junction resolvases, with intriguing connections to DNA replicati

298 Schizosaccharomyces pombe Holliday junction resolvase Ydc2 revealed significant structural homology

299 is insight, identified the Holliday junction resolvase Yen1 as a DNA repair target of Cdc14.

300 Control of the yeast HJ resolvase, Yen1, involves phosphorylation changes that m