ライフサイエンスコーパス: restriction enzyme

1 uced either by HO endonuclease or the I-SceI restriction enzyme.

2 the nonspecific cleavage domain of the FokI restriction enzyme.

3 is gap are substrates for Alu I, a blunt end restriction enzyme.

4 tracycline-controlled expression of the SacI restriction enzyme.

5 inated from bacterial modification-dependent restriction enzymes.

6 d with methylation-sensitive and insensitive restriction enzymes.

7 still no structural information on Type III restriction enzymes.

8 complexes and also with discussion of type I restriction enzymes.

9 endonucleases to function analogously to DNA restriction enzymes.

10 uperior accuracy and without the need to use restriction enzymes.

11 re of DNA sequences within the nucleosome to restriction enzymes.

12 single combined cluster analysis of multiple restriction enzymes.

13 ne modifications protect phage DNA from host restriction enzymes.

14 not require any restriction sites or use of restriction enzymes.

15 sk that rarely can be achieved using type-II restriction enzymes.

16 to protect phage DNA from a wide variety of restriction enzymes.

17 milarities between this and other classes of restriction enzymes.

18 e breakage of the P-O3' bond, in common with restriction enzymes.

19 kb DNA ladders when digested with two common restriction enzymes.

20 ragments are generated using frequent cutter restriction enzymes.

21 e limited in resolution by their reliance on restriction enzymes.

22 repeat as demonstrated by an increase in the restriction enzyme accessibility and in the size of DNA

23 e further substantiated by the findings of a restriction enzyme accessibility assay and TSA-stimulate

24 Restriction enzyme accessibility assay, DNase I footprin

25 easured by chromatin immunoprecipitation and restriction enzyme accessibility assays.

26 as demonstrated by micrococcal nuclease and restriction enzyme accessibility assays.

27 apping dynamics is associated with increased restriction enzyme accessibility of histone-bound DNA af

28 We have used FRET and restriction enzyme accessibility to study nucleosome dyn

29 Moreover, using restriction enzyme accessibility, we detect no changes i

30 Most restriction enzymes act as dimers with two catalytic sit

31 n might explain how other complex multimeric restriction enzymes act.

32 8)) in a gene cluster that encodes antiviral restriction enzyme activators (OAS1, OAS2 and OAS3); on

33 tiplex assay was applied to the detection of restriction enzymes activities as well as base excision

34 -molecule amplification; introduction of the restriction enzymes affected both the rate and the "fate

35 Type IIs restriction enzymes allow seamless assembly of synthons

36 Physical mapping of DNA with restriction enzymes allows for the characterization and

37 Restriction enzymes also enhanced integration events at

38 ion of the sequence-specific activity of the restriction enzyme Alu1.

39 he isolated DNA is suitable for digestion by restriction enzymes, amplification by PCR and Southern b

40 which was confirmed by methylation-dependent restriction enzyme analyses.

41 Restriction enzyme analysis and genomic sequencing ident

42 nd circulation periods were characterized by restriction enzyme analysis of viral DNA and select gene

43 n of methylation using methylation-sensitive restriction enzyme analysis or focused on single-copy ge

44 In contrast, FRET and restriction enzyme analysis reveal that only PTMs throug

45 e between 1997 and 2003 were genome typed by restriction enzyme analysis.

46 Briefly, genomic DNA is digested with a restriction enzyme and adapters are ligated.

47 Restriction enzyme and complete sequencing data demonstr

48 ique that involves cutting the genome with a restriction enzyme and isolating the targeted sequences.

49 representations from a methylation-sensitive restriction enzyme and its methylation-insensitive isosc

50 he chromatin is solubilized, digested with a restriction enzyme and ligated at low DNA concentration

51 y was used to test this model for the EcoRII restriction enzyme and provide direct visualization and

52 The HMW DNA is digested using an appropriate restriction enzyme and size-fractionated using pulsed-fi

53 which is greatly influenced by the choice of restriction enzyme and the frequency at which it can cut

54 n is structurally similar to classic Type II restriction enzymes and contains the endonuclease cataly

55 ross the Saccharomyces cerevisiae genome via restriction enzymes and DNA methyltransferases.

56 ecific factors, including selectivity of DNA restriction enzymes and fragmentation method, as well as

57 present a model for restriction by type III restriction enzymes and highlight the similarities betwe

58 going molecular cloning steps and the use of restriction enzymes and ligases necessary in other avail

59 bout recognition and cleavage sites for both restriction enzymes and methyltransferases as well as co

60 samples digested with methylation sensitive restriction enzymes and mock digested are then transform

61 ethod that depended on methylation-sensitive restriction enzymes and real-time RT-PCR.

62 n other translocases such as type I/type III restriction enzymes and SF1/SF2 helicases.

63 This review traces the discovery of restriction enzymes and their continuing impact on molec

64 among the most important characteristics of restriction enzymes and their corresponding methylases,

65 odels put forward for the action of type III restriction enzymes and their inadequacies.

66 was first digested by methylation-sensitive restriction enzymes and then precipitated by methyl-bind

67 ins how ArdA can bind and inhibit the Type I restriction enzymes and we demonstrate that 6 different

68 and at high resolution the DSBs induced by a restriction enzyme, and we characterize their impact on

69 -DNA precipitation and methylation-sensitive restriction enzymes, and laser-capture microdissection f

70 some structural insights into the working of restriction enzymes, and offers some preliminary data ne

71 tors of terminases, helicases, translocating restriction enzymes, and protein translocases possess a

72 se superfamily of proteins that include many restriction enzymes, and the structure of the active sit

73 plied to study both protein and nucleic acid restriction enzymes, and was demonstrated to accurately

74 s when we use data generated using different restriction enzymes, and when we reconstruct structures

75 s (PFGE) after digestion of genomic DNA with restriction enzyme ApaI.

76 the reduction in genomic complexity with the restriction enzymes approach, genotyping-by-sequencing.

77 The conclusion that type I restriction enzymes are catalytic relative to DNA has im

78 While many Type II restriction enzymes are dimers with a single DNA-binding

79 Cas proteins and restriction enzymes are unable to access the phage DNA t

80 We test this hypothesis using a restriction enzyme as a probe of chromatin structure and

81 nded an observed association, using a custom restriction-enzyme assay to analyze the DNA in 158 sampl

82 y, using a combination of Sanger sequencing, restriction enzyme assays and targeted deep sequencing.

83 Here we describe the first restriction-enzyme-assisted LC-MS/MS sequencing study of

84 prototypical DNA-binding protein, the PvuII restriction enzyme, at microfluidic-encapsulated, DNA-mo

85 es increased the computed accessibility to a restriction enzyme, at sites as distant as 40 nm, due to

86 uplex is substituted by RNA we find that six restriction enzymes (AvaII, AvrII, BanI, HaeIII, HinfI a

87 ng prevents endonuclease activity by type II restriction enzymes BamHI, EcoRI and SalI, and inhibitio

88 w that the CBASS effector NucC is related to restriction enzymes but uniquely assembles into a homotr

89 representation of every site of a particular restriction enzyme by short DNA tags.

90 The slow evolution and engineering of new restriction enzymes calls for alternative strategies to

91 The BcgI restriction enzyme can thus excise two DNA segments toge

92 native strategies to design novel and unique restriction enzymes capable of binding and digesting spe

93 e-wide analyses of DNA modifications rely on restriction enzymes capable of digesting genomic DNA at

94 ses are consistent with the observation that restriction enzymes caused extensive DNA cleavage in the

95 Mutant analysis by PCR and restriction enzyme cleavage (MAPREC) is used to measure

96 ion sites can be highly biased by the use of restriction enzyme cleavage of genomic DNA, which is a l

97 al analysis by polymerase chain reaction and restriction enzyme cleavage) assay for quality control o

98 sensitive or fully resistant to isoschizomer restriction enzyme cleavage.

99 s available for the systematic evaluation of restriction enzyme combinations that can enrich for cert

100 Bisulfite Sequencing (RRBS) protocol to all restriction enzyme combinations.

101 eover, we show in silico that cuRRBS-defined restriction enzymes consistently out-perform MspI digest

102 Thus, germline expression of mitochondrial restriction enzymes creates a powerful selection and has

103 Zinc finger nucleases are artificial restriction enzymes, custom-designed to cleave a specifi

104 sing confocal Raman spectra, S1 nuclease and restriction enzymes demonstrated that the structural dif

105 Zinc finger nucleases (ZFNs) are engineered restriction enzymes designed to target specific DNA sequ

106 ysis with hemoglobin is demonstrated through restriction enzyme detection, and an enhancement in sens

107 were screened for the c.828+3A>T mutation by restriction-enzyme digest, single-strand conformational

108 ntional cloning requires the purification of restriction-enzyme-digested vectors prior to the ligatio

109 antitative PCR (qPCR) and glucosyl-sensitive restriction enzyme digestion (gRES-qPCR).

110 bisulfite sequencing, methylation sensitive restriction enzyme digestion and array-based detection t

111 Standard BioBrick assembly normally involves restriction enzyme digestion and ligation of two BioBric

112 (scCGI-seq), combining methylation-sensitive restriction enzyme digestion and multiple displacement a

113 on and adduct bypass and a slightly modified restriction enzyme digestion and post-labeling assays re

114 ntrol modules capable of mutation sensing by restriction enzyme digestion and real-time on-chip micro

115 d from an avian erythroleukemia cell line by restriction enzyme digestion and released from the nucle

116 ally, we revert the ligation process using a restriction enzyme digestion and religate the resulting

117 A real-time RT-PCR pyrosequencing assay, a restriction enzyme digestion assay, and direct sequencin

118 for this hypothesis, we assessed, by using a restriction enzyme digestion coupled with LC-MS/MS metho

119 to pro-B cell lines and HS1 is accessible to restriction enzyme digestion exclusively in normal pro-B

120 Assessment of AMD variants was done by restriction enzyme digestion of PCR products and TaqMan

121 de polymorphism (SNP) assay based on PCR and restriction enzyme digestion or sequencing of the amplif

122 onverted to an expression vector by a simple restriction enzyme digestion with MfeI (to "drop-out" th

123 f the region encompassing the polymorphisms, restriction enzyme digestion, and detection of fragments

124 K, T, V, X, and U were characterized by PCR, restriction enzyme digestion, and/or sequencing.

125 The purified YAC DNA is suitable for restriction enzyme digestion, DNA sequencing and functio

126 enic epitope, as reported by diagnostic NciI restriction enzyme digestion, DNA sequencing, and wester

127 formation of the tetrahedron was verified by restriction enzyme digestion, Ferguson analysis, and ato

128 renal carcinoma cell lines was assessed with restriction enzyme digestion-coupled PCR and bisulfite g

129 parasites as determined by sequencing and/or restriction enzyme digestion.

130 according to their sequence similarities and restriction enzyme digestion.

131 (or representation) of the genome following restriction enzyme digestion.

132 as isothermal nucleic acid amplification and restriction enzyme digestion.

133 tients was performed by Sanger sequencing or restriction enzyme digestion.

134 RRBS involves restriction-enzyme digestion, bisulfite conversion and s

135 oint mutations uses nested PCR combined with restriction enzyme digestions, which is laborious and ti

136 comprehensive database of information about restriction enzymes, DNA methyltransferases and related

137 comprehensive database of information about restriction enzymes, DNA methyltransferases and related

138 The Type II restriction enzymes (e.g. EcoRI) gave rise to recombinan

139 e DNA-linked assembly of nanoparticles using restriction enzymes (e.g., MspI).

140 Our model system, restriction enzyme Ecl18kI, interacts with a FRET pair-l

141 tic measurements: the cleavage of DNA by the restriction enzyme EcoRV.

142 C is one of the three modification-dependent restriction enzymes encoded by the Escherichia coli K12

143 All restriction enzymes examined are phosphodiesterases gene

144 After a short pulse of restriction enzyme expression followed by a long period

145 There are hundreds of restriction enzyme families but few are used to date, be

146 EcoP15I is the prototype of the Type III restriction enzyme family, composed of two modification

147 ific DNA nucleases produced by fusion of the restriction enzyme FokI endonuclease domain (FN) with th

148 Lastly, we show that a proper choice of restriction enzyme for the optical map may substantially

149 GB2.0 relies on the use of type IIS restriction enzymes for DNA assembly and proposes a modu

150 , prediction of nonsense-mediated decay, and restriction enzymes for RFLP analysis.

151 press the yeast HO endonuclease or bacterial restriction enzymes for single or multiple DSB formation

152 ots can be released by simple digestion with restriction enzymes for subsequent characterization by s

153 target, queries over 1.3 M independent NspI restriction enzyme fragments in the 200 bp to 1100 bp si

154 Here, we report a restriction enzyme-free approach to library generation u

155 this approach include facile amplification, restriction enzyme-free library generation, and a signif

156 d-type levels of homologous recombination at restriction enzyme-generated breaks but is deficient in

157 Active restriction enzyme genes have been identified, and their

158 t this is a reliable method to detect active restriction enzyme genes in newly sequenced genomes, the

159 ide containing the optimal substrate for the restriction enzyme GlaI.

160 ngle-insert cloning of DNA fragments without restriction enzymes has traditionally been achieved usin

161 While restriction enzymes have advanced genomic analysis, and

162 Ionizing radiation and restriction enzymes have been shown to increase the freq

163 eened for this particular mutation using the restriction enzyme HhaI.

164 e developed HiCap, which combines a 4-cutter restriction enzyme Hi-C with sequence capture of promote

165 We verify our method by using restriction enzyme Hind III to cleave the fluorescent ds

166 similar in structure to two other monomeric restriction enzymes, HinP1I (G CGC) and MspI (C CGG), wh

167 e performed a previously reported assay, the restriction enzyme hotspot mutation assay (IgkappaREHMA)

168 e predigested with the methylation-sensitive restriction enzyme HpaII.

169 ubtypes of CRISPR-Cas3, Cas9, Cas12a and the restriction enzymes HsdRMS and EcoRI.

170 in structure of the MHRE exhibited increased restriction enzyme hypersensitivity and enhanced histone

171 Using methylation-sensitive restriction enzymes hypomethylated maternal sequences ar

172 and breaks (DSBs) induced by a site-specific restriction enzyme, I-SceI or by ionizing radiation (IR)

173 nerated by inducible expression of the AsiSI restriction enzyme in normal human fibroblasts.

174 To explore the possibility of using restriction enzymes in a synthetic biology based on arti

175 he expression of > 600 genes was affected by restriction enzymes in cells lacking PT, including induc

176 al mechanism of PT-dependent DNA cleavage by restriction enzymes in the face of partial PT modificati

177 pESBL in new hosts by blocking the action of restriction enzymes, in an orientation-dependent fashion

178 , we report that both ionizing radiation and restriction enzymes increase the frequency of microhomol

179 We use two restriction enzymes independently to create a series of

180 Efficient cleavage of these 100-mers by a restriction enzyme indicates that the DNA adopts a nativ

181 A and render nucleosomal DNA accessible to a restriction enzyme, indicative of a chromatin-remodeling

182 y promote large-scale genomic deletions from restriction enzyme-induced DSBs; third, they are require

183 Indeed, we show that restriction-enzyme-induced double-strand breaks are suff

184 tion of the recognition sequence of a Type-I restriction enzyme is a complicated procedure.

185 (MSCC) with the HpaII methylation-sensitive restriction enzyme is a cost-effective method to pinpoin

186 Remosome DNA accessibility to restriction enzymes is also markedly increased.

187 four DNA fragments in the presence of eight restriction enzymes is demonstrated.

188 Restriction enzyme kinetic analysis suggests that these

189 Restriction enzyme KpnI is a HNH superfamily endonucleas

190 several algorithms and modules, updated the restriction enzyme library, added batch processing capab

191 eaction (PCR) and analyzed by sequencing and restriction enzyme mapping.

192 lease) was identified as an inhibitor of the restriction enzyme McrBC.

193 Amongst restriction enzymes, McrBC and its close homologues are

194 In one mutant, the restriction enzyme-mediated integration cassette disrupt

195 A screen of restriction enzyme-mediated integration mutagenized cell

196 The procedure consists of three steps: a restriction enzyme-mediated ligation of an adapter to th

197 bisulfite conversion, methylation-sensitive restriction enzymes, methyl-binding proteins and anti-me

198 tation (MeDIP-seq) and methylation-sensitive restriction enzyme (MRE-seq) sequencing data to predict

199 ombined MeDIP-seq with methylation-sensitive restriction enzyme (MRE-seq) sequencing for comprehensiv

200 A is digested by the methylation-insensitive restriction enzyme MspI to generate short fragments that

201 ibe the application of methylation-sensitive restriction enzymes (MSRE) and quantitative PCR (qPCR) t

202 age with a cocktail of methylation-sensitive restriction enzymes (MSREs).

203 ning the use of designed ZFNs and commercial restriction enzymes, multiple plant expression cassettes

204 No restriction enzymes, mutagenesis of internal restriction

205 A, cDNA is synthesized and digested with the restriction enzyme NlaIII.

206 While many restriction enzymes operate symmetrically at palindromic

207 Numerous techniques using either restriction enzyme or affinity-based approaches have bee

208 reporter plasmids in Escherichia coli using restriction enzymes or sgRNA/Cas9 DNA scission to capita

209 ted bacterial DNA, samples are fragmented by restriction enzymes or sonication, then thermocycled in

210 randed molecules of genomic DNA using either restriction enzymes or sonication.

211 es in buffer composition and the presence of restriction enzymes or specific nucleic acids.

212 he use of methylation-sensitive isoschizomer restriction enzyme pairs and/or sodium bisulfite treatme

213 hain reaction, bisulfite pyrosequencing, and restriction enzyme-polymerase chain reaction.

214 We have used a restriction enzyme protection, selection and amplificati

215 use model expressing a mitochondria-targeted restriction enzyme, PstI or mito-PstI, to damage mitocho

216 use model expressing a mitochondria-targeted restriction enzyme, PstI or mito-PstI.

217 t mapping method that combines 5hmC-specific restriction enzyme PvuRts1I with a 5hmC chemical labelin

218 We performed methylation-sensitive restriction enzyme-quantitative PCR (MSRE-qPCR) and obse

219 ifies the ATP hydrolysis scheme for Type III restriction enzymes, questions remain as to why multiple

220 modification genes and an adjacent putative restriction enzyme (RE) operon likely form a restriction

221 lecular manipulation methods, we investigate restriction enzyme reactions with double-stranded (ds)DN

222 Type I restriction enzymes (REases) are large pentameric protei

223 e allele read frequency, strain specificity, restriction enzyme recognition site changes and flanking

224 ds have been extended to include fifteen new restriction enzyme recognition sites.

225 death by cleaving the E. coli genome at the restriction enzyme recognition sites.

226 on a user-defined template, (3) selection of restriction-enzyme recognition sites in the spacer betwe

227 DNA cleavage by the Type III restriction enzymes requires long-range protein communic

228 erases generating 3-OH and 5-P ends, but one restriction enzyme (restriction glycosylase) excises unm

229 complementary method, methylation-sensitive restriction enzyme sequencing (MRE-seq).

230 Restriction enzymes share little or no sequence homology

231 ation of chromosomal DNA with McrBC and DpnI restriction enzymes, single-stranded cDNA (ss-cDNA) liga

232 ed representation sequencing approach called Restriction Enzyme Site Comparative Analysis (RESCAN) to

233 nctions without constraints being imposed by restriction enzyme site location.

234 The ELAN method uses judicious choice of restriction enzyme sites coupled with simultaneous diges

235 lculated by using an RNA 3 reporter carrying restriction enzyme sites created by introducing silent m

236 plified by flanking primers that can include restriction enzyme sites for inserting the product into

237 rimarily occurs within two consecutive HpaII restriction enzyme sites in a tissue-specific manner, mo

238 ever, Clustal DNA alignments identified AFLP restriction enzyme sites that were undigested in the tis

239 To facilitate mutagenesis reactions, restriction enzyme sites were introduced in the tandem g

240 with single UV lesion surrounded by multiple restriction enzyme sites, we demonstrate in vitro that D

241 230 bp DNA segment containing five pairs of restriction enzyme sites, which can be used to produce a

242 r" particles, interacted with a DNA-cleaving restriction enzyme, SmaI, whose activity degraded the dr

243 f the cloned gmr genes in vivo and suggest a restriction enzyme specific for sugar modified HMC DNAs.

244 ification (RT-LAMP), and linear degradation (restriction enzymes) starting with hepatitis C viral RNA

245 0's DNA is resistant to cutting with several restriction enzymes, suggesting DNA modification, but de

246 sites including options for the selection of restriction enzyme suppliers, and (4) output files desig

247 restriction analysis (ARDRA) recruiting the restriction enzyme Taq I and compared to the virtually d

248 ral and regulatory chromatin interactions by restriction enzyme targeting and two-step proximity liga

249 MmeI is an unusual Type II restriction enzyme that is useful for generating long se

250 TaqI is a 263-amino acid (aa) Type IIP restriction enzyme that recognizes and cleaves within th

251 Tth111II is a thermostable Type IIGS restriction enzyme that recognizes DNA sites CAARCA (R =

252 EcoP15I is a type III restriction enzyme that requires two recognition sites i

253 inc finger nucleases (ZFNs)-hybrid synthetic restriction enzymes that can be specifically designed to

254 MspJI belongs to a family of restriction enzymes that cleave DNA containing 5-methylc

255 ctivity following BAC digestion with several restriction enzymes that cleaved different sets of genes

256 The product is digested by restriction enzymes that cut at unique sites between the

257 The restriction enzymes that cut DNA after interacting with

258 The standard mode of action for the restriction enzymes that excise their recognition sites

259 al that BsrDI and BtsI belong to a family of restriction enzymes that possess two catalytic sites: a

260 The structure of AgeI is similar to the restriction enzymes that share in their target sites a c

261 loci encoding type IV methylation-dependent restriction enzymes that target DNA containing C5-methyl

262 ures offer a rare opportunity to compare two restriction enzymes that work on exactly the same DNA su

263 In contrast to restriction enzymes the Fenton reaction is known to clea

264 he BcgI endonuclease exemplifies a subset of restriction enzymes, the Type IIB class, which make two

265 age threshold for permeation of DNA bound to restriction enzymes through a nanopore that is associate

266 BRISK utilizes a Type IIB DNA restriction enzyme to create a defined representation of

267 ation of random fragmentation and a type III restriction enzyme to derive a densely covering sgRNA li

268 situ DNase Hi-C obviates the dependence on a restriction enzyme to digest chromatin, instead relying

269 PvuRts1I was the first identified restriction enzyme to exhibit specificity toward hmC ove

270 The program also finds the best restriction enzyme to further diversify HRM or FLP signa

271 me that expresses a mitochondrially targeted restriction enzyme to induce tissue-specific homoplasmy

272 Targeting a restriction enzyme to mitochondria in the germline compr

273 be combined with the nuclease domain of FokI restriction enzyme to produce TAL effector nucleases (TA

274 ave developed modification-dependent type IV restriction enzymes to defend the cell from T4-like infe

275 samples, reduces genome complexity by using restriction enzymes to divide the genome into fragments

276 ures of DGS include (1) use of high-frequent restriction enzymes to fractionate the genome into small

277 hich micrococcal nuclease is used instead of restriction enzymes to fragment chromatin, enabling nucl

278 We leverage codon degeneracy and type IIs restriction enzymes to generate orthogonal ligation link

279 method of genome reduction that employs two restriction enzymes to generate overhangs in opposite or

280 PEER uses adapters and class IIS restriction enzymes to generate tagged oligonucleotides

281 tion analysis, in which OTU DUBs are used as restriction enzymes to reveal linkage type and the relat

282 oma-derived variable light transcripts using restriction enzyme treatment.

283 ubsequent restriction analysis with a single restriction enzyme, Tsp5091, yielded distinct RFLP patte

284 protein can be purified and forms an active restriction enzyme upon addition of restriction subunits

285 genomic DNAs treated with four endonuclease restriction enzymes using both chamber and droplet dPCR

286 The new class of modification-dependent restriction enzymes was named Type IV, as distinct from

287 In the presence of restriction enzymes, we directly visualize the cleavage

288 G2-C-G3-C-C recognition sequence of the NarI restriction enzyme were compared, using the oligodeoxynu

289 ease C gene of H. pylori and Sau-3 and Hha I restriction enzymes were used for polymerase chain react

290 When multiple restriction enzymes were used to map the transgene posit

291 Among this set are genes encoding restriction enzymes which, when active in E. coli, lead

292 esearch on zinc finger proteins and the FokI restriction enzyme (which revealed a bipartite structure

293 In this study, we use the EcoRII restriction enzyme, which employs a three-site binding m

294 ining numerous pores, allowing the access of restriction enzymes while preventing the DNA from physic

295 Engineering restriction enzymes with new sequence specificity has be

296 The creation of restriction enzymes with programmable DNA-binding and -c

297 analysis by analyzing the cleavage sites of restriction enzymes within exon 1 of the AR gene and fou

298 PFGE profiles were generated by use of six restriction enzymes (XbaI, BlnI, SpeI, SfiI, PacI, and N

299 roved T-vector system takes advantage of the restriction enzyme XcmI to generate a T-overhang after d

300 q to determine the frequency and spectrum of restriction-enzyme-, zinc-finger-nuclease-, and RAG-indu