ライフサイエンスコーパス: restriction site

1 odification (e.g. the addition or removal of restriction sites).

2 nto unit-length genomes when cut at a unique restriction site.

3 istribution of ApaI conformations around the restriction site.

4 and catalytic activity and abrogates a DraI restriction site.

5 model for a DNA decamer containing the EcoRI restriction site.

6 he opposite strand just outside of the PvuII restriction site.

7 ible for the presence or absence of the PstI restriction site.

8 ve; all were due to point mutations within a restriction site.

9 d by O6-methylguanine, positioned within the restriction site.

10 DR method requires the introduction of a new restriction site.

11 vivo compared with individuals lacking this restriction site.

12 ase I-SceI that recognizes and cuts an 18-bp restriction site.

13 cific bases in a sequence and generate a new restriction site.

14 indexing sequence located juxtaposed to the restriction site.

15 -2, which causes a gain or loss of an NlaIII restriction site.

16 ragments that do not have a common or unique restriction site.

17 l tags that can be derived from a predefined restriction site.

18 108, 310 and 350pb), due to a conserved BsaI restriction site.

19 281 bp) due to a 16 S rDNA conserved Hinf I restriction site.

20 npredicted HinfI RFLP, resulting in an EcoRI restriction site.

21 wed absence of the conserved 16 S rDNA HinfI restriction site.

22 ctinomycin (Spc) and causes loss of one HpaI restriction site.

23 le changes typically involve the revision of restriction sites.

24 re facilitated by a repeated set of flanking restriction sites.

25 y cut lambda-phage DNA molecules at specific restriction sites.

26 ed a duplicated sgp region flanked by marker restriction sites.

27 h A and D genomes but differently flanked by restriction sites.

28 to digestion since it does not contain these restriction sites.

29 ion-PCR followed by digestion at polymorphic restriction sites.

30 ng recombinant molecules without reliance on restriction sites.

31 lity of the different nucleotides within the restriction sites.

32 d SVs with or without creating or destroying restriction sites.

33 into the PCR product to protect most of the restriction sites.

34 gene construction in the absence of suitable restriction sites.

35 ithout the limitation of naturally occurring restriction sites.

36 tifies genomes encoding systems with similar restriction sites.

37 such a family uses a specific combination of restriction sites.

38 on the creation of novel (SfiI) and (BstUI) restriction sites.

39 5-hmC at single base pair resolution at MspI restriction sites.

40 enic region that contains many commonly used restriction sites.

41 d by the limited availability of appropriate restriction sites.

42 a coli without dependence on suitably placed restriction sites.

43 vectors multiple cloning site with 10 unique restriction sites, (4) an oriT for conjugation-mediated

44 ions do not always occur within pre-existing restriction sites, a generalized PCR/RE/LDR method requi

45 solubility' mutations) flanked by convenient restriction sites, a translational enhancer, and a conse

46 ds and an antagonist showed that increase in restriction site accessibility correlates with transcrip

47 RA markedly increased restriction site accessibility within the promoter, incl

48 romatin structure, as revealed by changes in restriction site accessibility.

49 fficient algorithm to minimize the number of restriction sites against sets of cutter sequences, and

50 parative macrorestriction mapping using rare-restriction-site alleles (made with the Tn10dRCP2 family

51 The unique restriction sites allowed the insertion of test sequence

52 ated in order to evenly distribute 25 unique restriction sites along the length of the gene while ret

53 WGM utilizes restriction site analysis but arranges 200 to 500 bands

54 A chloroplast DNA (cpDNA) restriction site analysis of Argyranthemum (Asteraceae:

55 oduplex analysis, nucleotide sequencing, and restriction site analysis.

56 ng the sequence complementary to the cleaved restriction site and a 4-nucleotide 'indexing sequence.'

57 Several polymorphisms, including a BsmI restriction site and a poly(A) microsatellite, can be us

58 causes skipping of exon 5, deleting an RsaI restriction site and decreasing the amounts of mRNA foun

59 Both mutations disrupted a Taq I restriction site and restriction fragment length polymor

60 the oligonucleotide and the template at the restriction site and sufficient complementarity overall

61 I was exchanged for that of mBE I at a BspHI restriction site and was purified to homogeneity and cha

62 that now contains the NotI, AscI, and EcoRV restriction sites and allows the rapid cloning of NotI-E

63 This family of vectors has compatible restriction sites and modular design, so that the protei

64 In addition, for each possible target site, restriction sites and primer candidates are visualized,

65 sing appropriate combinations of polymorphic restriction sites and probes.

66 promoter type, restriction enzyme, number of restriction sites and with a noted exception, cell line.

67 e chain reaction primers containing internal restriction sites, and cloned into the phagemid pCOMB3H

68 d by the distribution of naturally occurring restriction sites, and requires minimal DNA sequencing.

69 3A26 hybrids were generated using convenient restriction sites, and site-directed mutagenesis was use

70 sequences lying adjacent to a given Type IIS restriction site are ligated between two DNA adapters.

71 Due to the reduced size, more unique restriction sites are available in the MCS, thus allowin

72 chromosome size, gene order, and some of the restriction sites are conserved between the two phage gr

73 loped a novel physical assay in which HaeIII restriction sites are positioned within the telomeric tr

74 Restriction sites are significantly underrepresented in

75 The spacer sequences between the nicking and restriction sites are tailored to create ss extensions o

76 ipts containing fewer than two of the chosen restriction sites are undetectable by cDNA-RDA.

77 It was concluded that point mutations at restriction sites are unlikely to account for the restri

78 tes containing the same lesion and strategic restriction sites around the lesion.

79 ion sites, whereas R recognizes unmethylated restriction sites as non-self and introduces a double-st

80 We therefore used restriction site associated DNA (RAD) sequencing to accu

81 We used restriction site associated DNA (RAD) sequencing to disc

82 Restriction site associated DNA (RAD) tags are a genome-

83 ale biological sampling and phenotyping with restriction site associated DNA sequencing (RAD-Seq) dat

84 We used restriction site associated DNA sequencing to generate g

85 worldwide collections of P. triticina using restriction site associated genotyping by sequencing.

86 We tested the potential of double digested restriction-site associated DNA (ddRAD) sequencing for d

87 ion sequencing data were generated using the restriction-site associated DNA (RAD-seq) method.

88 gant analysis (BSA) version of double-digest restriction-site associated DNA sequencing (BSA-ddRADseq

89 n-model datasets generated via double digest restriction-site associated DNA sequencing (ddRAD).

90 We utilize fossil data and restriction-site associated DNA sequencing (RAD-seq) for

91 We used restriction-site associated DNA sequencing (RAD-seq) to

92 Using double-digest restriction-site associated DNA sequencing, we surveyed

93 nation of flow cytometry and high-throughput restriction-site associated DNA tag sequencing, we show

94 , and eastern) via analysis of double digest restriction-site associated DNA using Next Generation Se

95 We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method

96 l wing-pattern variants, we used genome-wide restriction site-associated DNA (RAD) genotyping, tradit

97 First, we report our use of restriction site-associated DNA (RAD) polymorphism marke

98 By using restriction site-associated DNA (RAD) sequencing we were

99 e strategies include whole-genome, exome and restriction site-associated DNA (RAD) sequencing.

100 gle-nucleotide variants (SNVs) by sequencing restriction site-associated DNA (RAD) tags in 138 Micant

101 gle-nucleotide variants (SNVs) by sequencing restriction site-associated DNA (RAD) tags in 138 Micant

102 We use quantitative trait loci analysis of restriction site-associated DNA -tag single nucleotide p

103 enerated and analyzed nuclear (double-digest restriction site-associated DNA fragment procedure [ddRA

104 pen source tool for assembling and analyzing restriction site-associated DNA sequence datasets using

105 Restriction site-associated DNA sequencing (RAD-seq) all

106 sity genetic maps of Zoysia japonica using a restriction site-associated DNA sequencing (RAD-Seq) app

107 ed out a genome-wide association study using restriction site-associated DNA sequencing (RAD-seq) of

108 types from more than 1,000 individuals using restriction site-associated DNA sequencing (RAD-seq).

109 High-throughput techniques based on restriction site-associated DNA sequencing (RADseq) are

110 Restriction site-associated DNA sequencing (RADseq) can

111 Large genomic data sets generated with restriction site-associated DNA sequencing (RADseq), in

112 otide polymorphism data were produced from a restriction site-associated DNA sequencing protocol for

113 lizing PacBio long reads in combination with restriction site-associated DNA sequencing, and for dipl

114 ributed in Japan, based on the double-digest restriction site-associated DNA sequencing.

115 This application of restriction site-associated DNA tags (RAD tags) reveals

116 h as pooled barcoded amplicon sequencing and restriction site-associated DNA tags, should be used to

117 Neurospora, we have adapted microarray-based restriction-site-associated DNA (RAD) mapping for use wi

118 t of RILs from a dense set of semicodominant restriction-site-associated DNA (RAD) markers and use si

119 Then, twenty of the offspring were used for Restriction-site-Associated DNA Sequencing (RAD-Seq) to

120 Here, we use genomic-scale restriction-site-associated DNA sequencing (RADseq) data

121 We employed restriction-site-associated DNA sequencing and integrate

122 Restriction-site-associated genomic markers are a powerf

123 of 31 mayfly (Ephemeroptera) communities and restriction-site-associated-DNA sequencing in the four m

124 imers to artificially introduce or abolish a restriction site at the target SNP site.

125 lion base pairs in length and used to insert restriction sites at any given nucleotide in a microbial

126 This module contains unique restriction sites at approximately 80 base pair interval

127 Methylation of CpG residues at restriction sites at the 5' end of both genes on the mur

128 yeast; however, this approach is limited by restriction-site availability.

129 i and find that, in agreement with levels of restriction site avoidance, EcoRI, but not EcoRV, cleave

130 An assessment of the SmaI restriction site-based multiplex PCR (SmaI-multiplex PCR

131 enoviral expression vector containing unique restriction sites between the human CMV-IE promoter and

132 Alleles which differ in size or restriction sites between these two strains were found f

133 to this vector by introduction of compatible restriction sites by PCR.

134 Artificial restriction sites can be introduced in known DNA sequenc

135 35-fold-repetitive B1 locus identified three restriction sites capable of discriminating type I (mous

136 morphism (SNP) variations in and around SmaI-restriction sites (CCCGGG).

137 Using a combination of haplogroup-specific restriction site changes and control region nucleotide s

138 , including very high G+C content and enzyme restriction sites characteristic of an HTF-island, no TA

139 ed, six of which were identical for numerous restriction site characters to plastome types found amon

140 Chloroplast DNA restriction-site comparisons were made among 24 species

141 s approach facilitates cloning regardless of restriction site compatibility and overcomes an importan

142 r such that annealing becomes independent of restriction site compatibility.

143 etic resolution within the prior plastid DNA restriction site data, highlight plastid/nuclear incongr

144 ng genome-wide nuclear SNPs, and plastid DNA restriction site data.

145 ged constructs with no undesirable vector or restriction-site-derived amino acids added to the expres

146 lution has failed to eliminate many possible restriction sites despite selective pressure, thus motiv

147 laboratory CMV strains that contained unique restriction sites developed for this purpose.

148 A amplification products resolved diagnostic restriction site differences for I. fulva and I. hexagon

149 126 strains were obtained at 16 polymorphic restriction sites distributed on nine PCR fragments.

150 uction was verified by detection of a unique restriction site engineered into the SHRV genome between

151 The template has appropriate restriction sites engineered into it that can be digeste

152 I should facilitate the automation of cosmid restriction site fingerprinting needed for large-scale m

153 smid reverts mostly by +1 frameshifts in the restriction sites flanking the insert.

154 Unique eight base cutter restriction sites flanking the promoters and poly-adenyl

155 By mapping millions of restriction site-flanking reads back to the Escherichia

156 by the salt-bridges, does not seem to be the restriction site for a continuous water passage.

157 ng (a) a finely spaced DNA ladder carrying a restriction site for BamHI, (b) RNA translocation by DEx

158 orted, was 2988 A or C that introduces a new restriction site for Eco0109 I.

159 gle alpha-factor repeat was constructed with restriction sites for cassette mutagenesis flanking the

160 A borders without compromising the choice of restriction sites for cloning, since the pGreen cloning

161 , and, in some cases, identifying endogenous restriction sites for downstream molecular genetic appli

162 ion was performed using primers that amplify restriction sites for MspAI (A2 polymorphism-CYP17) and

163 onsensus sequence upstream of the undigested restriction sites for possible methyltransferase recogni

164 by PCR using primers containing appropriate restriction sites for subcloning into pTETnirB, which co

165 However, because the plasmid lacks restriction sites for the enzymes BlnI and I-CeuI, it wa

166 s quantitated as a function of the number of restriction sites for the restriction enzymes carried by

167 oxynucleotide complex protects an individual restriction site from methylation, thus limiting subsequ

168 y EcoRI and DraI enzymes at their respective restriction sites, G downward arrow AATTC and TTT downwa

169 s DNA hydrolysis by BstZ17 I at the enzyme's restriction site, GTA downward arrowTAC.

170 triction enzyme digestion whether any of the restriction sites have been altered.

171 hetic vector arms from which the undesirable restriction sites have been removed by point mutations.

172 on of a silent mutation that created an XhoI restriction site in exon 7.

173 d mutation, TER1-Bcl, which generates a BclI restriction site in newly synthesized telomeric repeats,

174 g a single-base change that creates a unique restriction site in one of a pair of alleles.

175 he single-nucleotide change, create a unique restriction site in one of the alleles.

176 A single Eco47III restriction site in the 5' flanking region differentiate

177 One aphid clone bore a variant HindIII restriction site in the Buchnera leucine plasmid (pAPEle

178 e near the end of an element and an adjacent restriction site in the flanking genomic DNA.

179 This sequence alteration introduces a PleI restriction site in the nonsyphilis strains and thus all

180 rsonii reference strain 21038 but had a DraI restriction site in the spacer.

181 e substitution abolished the conserved HinfI restriction site in the two PCR fragments with unpredict

182 All but one mutation altered a restriction site in the URO-D gene, permitting identific

183 The specific protection of only one of many restriction sites in a genome from inactivation by a cog

184 Phylogenetic analysis of restriction sites in Culaea mtDNAs and haplotype-definin

185 e antibody-DNA combinations by incorporating restriction sites in DNA.

186 kelihood analysis of independent polymorphic restriction sites in humans reveals a decrease in effect

187 We took advantage of native restriction sites in SERT to construct a chimeric transp

188 y is based on the deprotection of methylated restriction sites in synthetic oligonucleotides and can

189 ocations of the EcoRI, SacII, EagI, and NotI restriction sites in the clones, resulting in a high-res

190 Ninety percent of the potential restriction sites in the constant regions and 47% of the

191 ore unaffected by the availability of useful restriction sites in the flanking region.

192 o the appearance of PstI and loss of HindIII restriction sites in the pol region as a result of sever

193 he constant regions and 47% of the potential restriction sites in the variable regions were present i

194 codon optimization for any expression host, restriction site inclusion and exclusion, separation of

195 representing both minor gene editing events (restriction site insertion) to mimic gene correction, or

196 that facilitates the design of endonuclease restriction sites into the 5'-end of PCR primers.

197 orter system consisted of a series of unique restriction sites introduced into SV40 at nt 2666.

198 equence-targeted site in which the protected restriction site is embedded.

199 In this design, a restriction site is engineered in one of the two polymer

200 ifier: through additional silent mutations a restriction site is included or a previous one removed w

201 A PCR reaction is performed in which a restriction site is introduced in wild-type DNA by alter

202 The interconnecting restriction site junctions that are located at the ends

203 ic H1 gene (H1d) by introduction of suitable restriction sites just 5' of the TATA box and 3' of the

204 Methylation of NotI restriction sites located in HIC-1 was demonstrated in 2

205 Finally, analysis of phage L restriction site locations and a number of patches of nu

206 eumatoid arthritis patients and confirmed by restriction site mapping.

207 An approach to restriction-site mapping and contig building that uses f

208 uble-strand break and the donor chromosome's restriction site marker.

209 Restriction site markers in the chloroplast DNA reveal s

210 of locations at which RT switched templates, restriction site markers were introduced to divide the d

211 WH204 and WH221, containing eight different restriction site markers were used.

212 repeat was flanked by two different sets of restriction site markers.

213 odified HIV-1 (NL-Mme) containing a type IIS restriction site, MmeI, at the right end of viral DNA.

214 oligomer-based library with minimal need for restriction site modification of sequences in the target

215 methods that rely on uneven distributions of restriction site motifs in the DNA.

216 Of 117 restriction-site mutations shared among a subset of two

217 , the YAC clones revealed several additional restriction sites not previously detected in genomic DNA

218 single copy of Tn551 in the same chromosomal restriction site of both ISP479R and tPMPr transductants

219 ide duplex containing the iterated CG repeat restriction site of the NarI endonuclease has been deter

220 , we have developed protocols using the Notl restriction sites of any PAC or BAC clone to introduce a

221 Our analysis of DNA methylation-sensitive restriction sites of the 3' RR has revealed a similar mo

222 shifted in accordance with the locations of restriction sites on the construct.

223 iciency, nearly independent of the number of restriction sites on the plasmid.

224 drug resistance genes and of several unique restriction sites on the plasmids enabled rigorous genet

225 o either ineffective cleavage at the cognate restriction site or relaxed specificity allowing cleavag

226 n reaction primers, and does not require any restriction sites or use of restriction enzymes.

227 restriction enzymes, mutagenesis of internal restriction sites, or reamplification to add end homolog

228 limited by the presence of mutation-specific restriction sites, patients with neuropathy, ataxia and

229 Infrequent restriction site PCR (IRS-PCR) is a recently described D

230 d using genomic data generated by infrequent restriction site PCR and gene sequence analysis of the f

231 agment length polymorphism analysis, genomic restriction site polymorphism analysis, and plasmid cont

232 plogroups was surveyed through the published restriction site polymorphism and control region sequenc

233 In addition, a restriction site polymorphism in the nuclear rRNA ITS-1

234 Length and restriction site polymorphism within a 2.8-kb threonine-

235 An AluI restriction site polymorphism within intron 4 was used t

236 were consistently distinguished by a Tsp45I restriction site polymorphism.

237 The frequencies of the restriction site polymorphisms at the human phenylalanin

238 sample of 31 Australian isolates by assaying restriction site polymorphisms from 11 protein encoding

239 detect an unprecedented number of genomewide restriction site polymorphisms from two N. crassa strain

240 A total of 10 restriction site polymorphisms have been identified at t

241 The use of silent restriction site polymorphisms indicates that the freque

242 e type of experiment, two phages marked with restriction site polymorphisms were crossed.

243 DNA (400 bp of hypervariable region 1 and 14 restriction site polymorphisms) and Y-chromosome (20 bia

244 f 301 polymorphic markers, consisting of 228 restriction site polymorphisms, 63 microsatellites, two

245 A comparison with 30 restriction-site polymorphisms (RSPs) for which the ance

246 Variation is assessed by use of 30 autosomal restriction-site polymorphisms (RSPs), 60 autosomal shor

247 In the course of this study, restriction-site polymorphisms between 129S1 and CzechII

248 ing sequence position, U6 termination (TTT), restriction sites, polymorphisms and specificity.

249 mages and data are presented that locate six restriction sites, predicted from gel electrophoresis, o

250 performs the following tasks in gene design: restriction site prediction, codon optimization for any

251 Gains of restriction sites relative to a known human mtDNA sequen

252 lly silent single-base mutations that create restriction sites (restriction fragment length polymorph

253 two homologous templates carrying different restriction sites separated by 282 bp.

254 the two alleles based on a polymorphic EarI restriction site showed that the Lys and Arg alleles had

255 r characterization of six ESRV loci revealed restriction sites specific for JSRV.

256 relatively inexpensive method called nested restriction site-specific PCR (RSS-PCR) that generates a

257 CR-mediated generation of an artificial AluI restriction site specifically with the CRM197 DNA templa

258 with newly incorporated expressed polylinker restriction sites still functions as a repressor.

259 We present the results of a restriction site survey of variation at five loci in Dro

260 This mutation produces a novel BstNI restriction site that can be used to identify individual

261 R3 exon 15, that would disrupt a unique BbsI restriction site that includes the Lys650 codon.

262 it, as the T8399G mutation creates a unique restriction site that is not present in wild-type human

263 A probe and target DNA anneal to create a restriction site that is recognized by a strand-specific

264 A region contains five groups of rare-cutter restriction sites that define CpG islands.

265 tial fraction of subtelomeric DNA containing restriction sites that is not digested with enzymes such

266 nce of a variable methylation pattern at the restriction sites that prevented proper enzyme cleavage

267 ively demonstrating the marker mutations and restriction sites that were engineered into the componen

268 r sequence motifs besides the canonical GATC restriction sites, thereby expanding the utility of this

269 se H1 digests RNA that occludes the relevant restriction sites, thus preventing detection of these lo

270 (less than 3 kb); (ii) possess eleven unique restriction site to facilitate directional cloning; (iii

271 The length of duplex DNA from a unique restriction site to the single-stranded DNA region was t

272 utation sites sometimes present some natural restriction sites to differentiate the wild-type and mut

273 applying it to data from a recent survey of restriction site variation in the chloroplast genome of

274 No restriction site variation in the mitochondrial genome w

275 l mys subfamilies identified on the basis of restriction site variation occur in more than one specie

276 so enhanced integration events at random non-restriction sites via microhomology-mediated recombinati

277 nd methylation of a previously reported NotI restriction site was associated with dense CpG methylati

278 human motilin receptor containing 33 unique restriction sites was designed and stably coexpressed in

279 spectively, were analysed, and a total of 67 restriction sites was scored.

280 wo-amino acid linker (generated by fusion of restriction sites) was used to join VH and CH3.

281 polymorphism, which generates a unique BsrI restriction site, was detected at position 2073.

282 Using unique restrictions sites, we created additional chimeric molec

283 m the rates of recombination between the two restriction sites were 1 and 7% using Taq and Vent polym

284 P analysis also disclosed that the same AFLP restriction sites were digested in all of the fecal isol

285 the plasmids and because a number of unique restriction sites were present in the vector, studies we

286 , 8 affecting chromosome size and 3 altering restriction sites, were observed in these populations, w

287 s was constructed and five SNPs, residing in restriction sites, were selected for the development of

288 hylating short specific DNA sequences called restriction sites, whereas R recognizes unmethylated res

289 The loss of a Mnl1 restriction site, which results from the deletion, was u

290 lted from the occurrence in the VL cDNA of a restriction site, which was cleaved during construction

291 s of DNA sequence polymorphisms that disrupt restriction sites, which allows RAD tags to serve as gen

292 he triplet code to design genomes that avoid restriction sites while producing the same proteins as w

293 d have been able to measure the positions of restriction sites with a precision of approximately 1.5

294 gene were characterized at eight polymorphic restriction sites within the beta-globin gene cluster.

295 The lack of variation for the other restriction sites within the gene examined here indicate

296 This analysis showed that restriction sites within the gene were highly conserved,

297 Restriction sites within the region of homology allowed

298 The increased accessibility of restriction sites within these repetitive sequences is i

299 A survey of 24 restriction sites yielded 16 polymorphic sites and 21 di

300 pairs O6-methylguanine; PvuII cleaves at its restriction site, yielding a blunt-ended double strand,