ライフサイエンスコーパス: rickettsial

1 of immune responses by a member of the order Rickettsiales.

2 ticola, a Lysobacter sp., and members of the Rickettsiales.

3 reed that mitochondria arose from within the Rickettsiales.

4 s byproducts of reductive evolution in other Rickettsiales.

5 ly associated uncultivated Legionellales and Rickettsiales.

6 nsively studied in the Chlamydiales than the Rickettsiales.

7 nown about their role in the transmission of Rickettsiales.

8 SAR11 with most strains distantly related to Rickettsiales.

9 classifies all SAR11 strains identically as Rickettsiales.

10 obligate intracellular pathogen of the order Rickettsiales.

11 Transcription of the rickettsial 16S rRNA gene (rrs), of which there is only

12 an undescribed genus in the Midichloriaceae (Rickettsiales)(7,8) and has a genomic repertoire similar

13 of the R. rickettsii actin tail suggest that rickettsial ABM is mechanistically different from previo

14 intracellular nature, little is known about rickettsial ABM relative to Listeria and Shigella ABM sy

15 In the current study, we show that rickettsial abundance in the tick midgut increases once

16 study we examined the ultrastructure of the rickettsial actin tail by confocal, scanning electron, a

17 ckettsial OmpA, which has been implicated in rickettsial actin tail formation.

18 ynthesize AdoMet suggested the presence of a rickettsial AdoMet transporter.

19 ease spirochete Borrelia burgdorferi and the rickettsial agent Anaplasma phagocytophilum Collectively

20 NLRC4 inflammasome during infection with the rickettsial agent Anaplasma phagocytophilum Macrophages

21 and promote infection of the mildly virulent rickettsial agent Anaplasma phagocytophilum through the

22 -1 activity during host stimulation with the rickettsial agent Anaplasma phagocytophilum.

23 Ehrlichia chaffeensis, a tick-transmitted rickettsial agent, causes human monocyte/macrophage-trop

24 s, a tick-transmitted obligate intracellular rickettsial agent, causes human monocytic ehrlichiosis.

25 Ehrlichia chaffeensis, a tick-transmitted rickettsial agent, is responsible for human monocytic eh

26 ease spirochete Borrelia burgdorferi and the rickettsial agents Anaplasma phagocytophilum and A. marg

27 is endemic that had tested negative to other rickettsial agents.

28 egrative conjugative element named RAGE (for Rickettsiales amplified genetic element), previously ide

29 me 23 [ORF23]-dnaG-rpoD), which includes the rickettsial analog (ORF23-dnaG-rpoD) of the major macrom

30 ith 9 serum samples from patients with other rickettsial and ehrlichial infections.

31 They include 5 Rickettsiales and 4 Rhodospirillales, two orders that ha

32 racellular bacteria (obligates) belonging to Rickettsiales and Chlamydiales cause diseases in hundred

33 We show that the Rickettsiales and Holosporales (both groups of intracell

34 t SAR11 are monophyletic and related to both Rickettsiales and the ancestor of mitochondria.

35 In addition to the known rickettsial antigens, OmpA and OmpB, we identified trans

36 The Rickettsiales are a group of obligate intracellular vect

37 Rickettsiales are important zoonotic pathogens, causing

38 e mitochondrial branch was placed within the Rickettsiales as a sister to the combined Anaplasmatacea

39 Factors related to the initial rickettsial bacterial concentration are likely the main

40 rasite I. scapularis and determined that the rickettsial bacterium A. phagocytophilum and the spiroch

41 a burgdorferi and the obligate intracellular rickettsial bacterium Anaplasma phagocytophilum, which c

42 A rickettsial bacterium in the genus Wolbachia is the caus

43 It is also related to a Rickettsial bacterium that causes male-killing in an unr

44 ants, providing a new tool for understanding rickettsial biology and furthering advances in the preve

45 ignal through TLR4 had significantly greater rickettsial burdens in vivo.

46 ae-stimulated DCs protected mice from lethal rickettsial challenge by limiting rickettsial proliferat

47 ective immunity against an ordinarily lethal rickettsial challenge, but the mechanism underlying this

48 Hence, these data suggest that Rickettsiales circulate widely in mosquitoes in nature.

49 iated killing in mammalian blood, a means of rickettsial clearance that has not been previously descr

50 o endothelial cells on day 10 at the time of rickettsial clearance.

51 invasion assays suggested that DvKPI limits rickettsial colonization during host cell entry.

52 o studies show that D. variabilis KPI limits rickettsial colonization of L929 cells (mouse fibroblast

53 Ticks must control rickettsial colonization to avoid immediate death.

54 out mice dramatically reduced the infectious rickettsial content in the organs, confirming that CD8 T

55 The source of rickettsial CTP appears to be the transport of UMP follo

56 splenic macrophages and intraphagolysosomal rickettsial death and digestion.

57 phagosome, resulting in intraphagolysosomal rickettsial death.

58 The expression of rickettsial dinucleoside oligophosphate pyrophosphatase

59 Rickettsial disease (RD) is a prevalent and underestimat

60 the first vaccine used to protect against a rickettsial disease and is still in widespread use a cen

61 ferent aspects of the spotted fever group of rickettsial disease establishment.

62 ytic anaplasmosis can be found in the recent rickettsial disease guideline developed by the Centers f

63 Rickettsial disease has been commonly associated with re

64 Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accu

65 Cowdria ruminantium causes the tick-borne rickettsial disease of heartwater, which is devastating

66 Bovine anaplasmosis is a rickettsial disease of world-wide economic importance ca

67 ehrlichiosis, a recently described emerging rickettsial disease, has been established by serial bloo

68 Rickettsial diseases (RDs) are transmitted to humans by

69 Rickettsial diseases are transmitted by arthropods and c

70 Recently, several novel agents of rickettsial diseases have been described.

71 Rickettsial diseases have long been diagnosed with serum

72 to the host resistance or susceptibility to rickettsial diseases, we first characterized the in vitr

73 f Atg5 in macrophages to the pathogenesis of rickettsial diseases.

74 uld be exploited to develop vaccines against rickettsial diseases.

75 rickettsiosis (TGR), and 1,193 (8.0%) other rickettsial diseases.

76 ck populations and the ecology of tick-borne rickettsial diseases.

77 al and non-natural outbreaks or epidemics of rickettsial diseases.

78 rickettsiae in cell culture, the recovery of rickettsial DNA following different methods of extractio

79 lso exhibited the greatest concentrations of rickettsial DNA from heart, lung, liver, and spleen samp

80 The results of this first rickettsial DNA microarray may provide some important in

81 a similar course of illness with a sublethal rickettsial dose, cleared the infection by day 10, and r

82 a diverse set of previously uncharacterized rickettsial effectors and lays the foundation for a deep

83 ce on the host cell has impeded discovery of rickettsial effectors and their host targets.

84 We here report on the novel genome of the rickettsial endosymbiont of Trichoplax sp. H2 (strain "P

85 In addition, O. volvulus contains the rickettsial endosymbiont Wolbachia, which has molecules

86 t mitochondria most likely originated from a Rickettsiales endosymbiont already residing in the host,

87 2)) has long been proposed to be involved in rickettsial entry into host cells, escape from the phago

88 f R. rickettsii-infected cells shortly after rickettsial exposure; and (iii) fluorescence-activated c

89 eutics for this and related pathogens of the rickettsial family Anaplasmataceae.

90 maternally transmitted microorganism of the Rickettsial family, is known to cause cytoplasmic incomp

91 Anaplasma marginale (order Rickettsiales, family Anaplasmataceae), a tick-borne pat

92 anching alphaproteobacterial orders were the Rickettsiales, followed by the Rhodospirillales and then

93 g a role for GpsA in vivo as part of a novel rickettsial G3P acquisition pathway for phospholipid bio

94 ification of a conserved spotted fever group rickettsial gene (ompA) followed by DNA sequencing of th

95 the feasibility of generating site-directed rickettsial gene mutants, providing a new tool for under

96 However, the corresponding rickettsial gene(s) encoding a protein with PLA(2) activ

97 ity of site-directed knockout mutagenesis of rickettsial genes and to generate a nonrevertible vaccin

98 We present evidence that conserved rickettsial genes associated with an intracellular lifes

99 ,496 bp) and compare it to the two published rickettsial genome sequences: R. prowazekii and R. conor

100 Bioinformatics analysis of over 30 Rickettsiales genome sequences illustrates a conserved c

101 The annotations of several rickettsial genomes indicate the presence of homologs of

102 The three rickettsial genomes share 775 genes: 23 are found only i

103 Unsurprisingly, large portions of Rickettsiales genomes encode proteins involved in transp

104 way compensates for the evolutionary loss of rickettsial glycolysis/gluconeogenesis, the typical endo

105 Purified recombinant rickettsial GpsA was shown to specifically catalyze the

106 Expression of rickettsial groEL, a molecular indicator of cellular str

107 Rickettsia but significantly worse with the rickettsial groups Ehrlichia and Orientia.

108 of interesting proteins possibly involved in rickettsial growth and virulence in mammalian cells.

109 0 microM alpha-lipoic acid did not influence rickettsial growth in endothelial cells, nor did it affe

110 LR4 function, whereas in TLR4-competent mice rickettsial growth manifested a lag phase early, suggest

111 Rickettsial growth proceeded logarithmically in mice lac

112 yme that synthesizes SAM, is unnecessary for rickettsial growth.

113 lifestyle, Alphaproteobacteria of the order Rickettsiales have inextricably coevolved with their var

114 Taken together, our data suggest that the rickettsial homology domain of IcsA is required for the

115 fferential expression profile of invA during rickettsial host cell infection was examined.

116 ly, suggesting that TLR4 may initiate innate rickettsial immunity.

117 nt role in the transmission and evolution of Rickettsiales in nature.

118 tick-borne bacterial pathogens in the order Rickettsiales, including Anaplasma phagocytophilum, Ehrl

119 (a scavenger of extracellular NO) during the rickettsial infection alleviated the suppression of the

120 may also contribute to the establishment of rickettsial infection and resulting pathogenesis.

121 contribute to the generation of a sustained rickettsial infection and subsequent disease have yet to

122 r interleukin-12 (IL-12) p40 production upon rickettsial infection and were more potent in priming na

123 mune regulatory mechanisms involved in fatal rickettsial infection have been unknown.

124 Thus, type I IFNs are induced during a rickettsial infection in vivo and promote severe disease

125 Delineating the molecular mechanisms of rickettsial infection is critical to a thorough understa

126 on alleviated the suppression of the initial rickettsial infection observed in appropriately treated

127 Rickettsial infection should be considered in the differ

128 activity was more critical to recovery from rickettsial infection than were the effects of IFN-gamma

129 product of NO) were produced and the initial rickettsial infection was suppressed in cultures of L929

130 Host cells responded to rickettsial infection with increased secretion of proinf

131 charide exhibited suppression of the initial rickettsial infection, and the suppression was relieved

132 travelers returning from sub-Saharan Africa, rickettsial infection, primarily tick-borne spotted feve

133 To address if fleas combat rickettsial infection, we characterized the cat flea (Ct

134 factors associated with the tick response to rickettsial infection, we utilized differential-display

135 hermore, NK cells are involved in preventing rickettsial infection-induced endothelial cell damage, p

136 ntibody-mediated killing confers immunity to rickettsial infection.

137 tress-induced toxic nucleotide levels during rickettsial infection.

138 CTLs appear to be crucial to recovery from rickettsial infection.

139 s was not the explanation for the control of rickettsial infection.

140 and prevented the suppression of the initial rickettsial infection.

141 te production and suppression of the initial rickettsial infection.

142 cells indicated IMD pathway activation upon rickettsial infection.

143 the protective inflammatory response during rickettsial infection.

144 Although survivors of rickettsial infections are considered immune to disease,

145 Many aspects of rickettsial infections have been characterized, includin

146 ions may be as common as spotted fever group rickettsial infections in febrile patients from central

147 Human cells are capable of controlling rickettsial infections intracellularly, the most relevan

148 smission model of the spotted fever group of rickettsial infections to study the initial events in di

149 TLR4 signaling developed overwhelming, fatal rickettsial infections when given an inoculum that was n

150 d against the major infected target cells of rickettsial infections, endothelial cells and macrophage

151 the Lao People's Democratic Republic (Laos), rickettsial infections, including scrub and murine typhu

152 , in relevant animal models that mimic human rickettsial infections, there is reciprocal immunologica

153 s of the immune response, remains unclear in rickettsial infections.

154 educe inflammatory damage to the host during rickettsial infections.

155 ole early during the immune response against rickettsial infections.

156 recently reclassified bacteria in the order Rickettsiales, infects many different animal species and

157 Confirmation of the rickettsial influence on the differential expression in

158 expression pattern during various stages of rickettsial intracellular growth was investigated.

159 expression pattern during various stages of rickettsial intracellular growth.

160 revealed several tick proteins important for rickettsial invasion, including actin filaments, actin-r

161 This rickettsial IS element appears to be active in that comp

162 Ehrlichia chaffeensis, a tick-transmitted rickettsial, is the causative agent of human monocytic e

163 A rickettsial isolate (isolate MOAa) belonging to the spot

164 The SFG rickettsial isolate could not be stably passaged in cell

165 the rompB gene) of MOAa and WB-8-2, another rickettsial isolate from A. americanum.

166 atory in Vientiane, Laos, routinely performs rickettsial isolation from hospitalized patients with su

167 ver, modifiable factors do contribute to the rickettsial isolation success, especially delays in inoc

168 Rickettsial killing in the human macrophage cell line wa

169 rickettsiae relative to the levels for other rickettsial lineages.

170 t if infection severity is a function of the rickettsial load delivered during tick transmission, the

171 al-time PCR (qPCR) was performed to estimate rickettsial load in heart, lung, spleen, and liver tissu

172 t the results of this study suggest that the rickettsial load in ticks and during transmission may be

173 thods of extraction, and the quantitation of rickettsial loads in infected animal tissues, clinical s

174 6) controls and was accompanied by increased rickettsial loads in various organs.

175 st likely driven by interaction of TLR4 with rickettsial LPS, contributes to host protective immunity

176 However, no rickettsial lysine methyltransferase has been characteri

177 The production and characterization of rickettsial lysine methyltransferases provide new tools

178 oratory cultured mosquitoes, suggesting that Rickettsiales may be maintained in mosquitoes through bo

179 structural and mechanistic insights into how rickettsial methyltransferases catalyze OmpB methylation

180 Rickettsial methyltransferases RP789 and RP027-028 are t

181 e revealed the presence of a mutation in the rickettsial metK gene, the gene encoding the enzyme resp

182 n mutant was constructed in which individual rickettsial metK genes were tested for the ability to co

183 The rickettsial MMSO consists of an ORF coding for a protein

184 Intracytoplasmic rickettsial morulae were detected on peripheral smear an

185 ith R. rickettsii to assess their effects on rickettsial motility.

186 is detectable early and prior to significant rickettsial multiplication and much earlier than the ult

187 ining intracellular pools of nucleotides for rickettsial nucleic acid biosynthesis and do not provide

188 quence similarity to two repeated domains of rickettsial OmpA, which has been implicated in rickettsi

189 Methylation of rickettsial OmpB (outer membrane protein B) has been imp

190 We identified the autotransporter protein rickettsial OmpB (rOmpB) as a factor H ligand and furthe

191 on between methylation of lysine residues in rickettsial OmpB and bacterial virulence has suggested t

192 ies focusing on Lyme disease spirochetes and rickettsial or tularemia agents as models for extracellu

193 Obligate intracellular bacteria of the Rickettsiales order have evolved to colonize both arthro

194 place mitochondria unequivocally within the Rickettsiales order, as a sister clade to the Rickettsia

195 n cells infected with Coxiella burnetii, the rickettsial organism that causes Q fever.

196 The tick-borne rickettsial organism, Anaplasma marginale, causes a dise

197 Ehrlichia chaffeensis, a tick-borne rickettsial organism, causes the disease human monocytic

198 chnology for rapid detection of as few as 10 rickettsial organisms in complex biological samples.

199 ngle 2.0x2.0x0.3 mm PAM filter, as few as 10 rickettsial organisms per 100 microl of lysed blood samp

200 of an important but neglected member of the Rickettsiales, Orientia tsutsugamushi.

201 ransfused with monoclonal antibodies against rickettsial outer membrane protein A (OmpA), OmpB, or li

202 A major surface antigen, rickettsial outer membrane protein A (rOmpA), is severel

203 tes rompA, encoding a major surface antigen (rickettsial outer membrane protein A [rOmpA]) and member

204 eport here that the autotransporter protein, rickettsial outer membrane protein B (rOmpB), constitute

205 e used PCR to characterize the genes for the rickettsial outer membrane proteins rOmpA and rOmpB.

206 id-phase complement regulators and conserved rickettsial outer membrane-associated proteins are criti

207 has a genomic repertoire similar to that of rickettsial parasites(9,10), but does not seem to expres

208 The rickettsial pathogen Anaplasma marginale assembles an ac

209 The rickettsial pathogen Anaplasma marginale establishes lif

210 The rickettsial pathogen Anaplasma marginale expresses a var

211 anaplasmosis caused by the intraerythrocytic rickettsial pathogen Anaplasma marginale is endemic in S

212 The tick-borne rickettsial pathogen Anaplasma phagocytophilum develops

213 In the rickettsial pathogen Ehrlichia chaffeensis, the virBD ge

214 Anaplasma marginale is an intraerythrocytic rickettsial pathogen of cattle in which infection persis

215 gent of bovine anaplasmosis, is a tick-borne rickettsial pathogen of cattle that multiplies in erythr

216 Anaplasma marginale, a tick-borne rickettsial pathogen of cattle, infects bovine erythrocy

217 naplasma platys is an obligate intracellular rickettsial pathogen that infects platelets of dogs, for

218 Anaplasma phagocytophilum is a tick-borne rickettsial pathogen that provokes an acute inflammatory

219 between molecular pathways manipulated by a rickettsial pathogen to survive in its arthropod vector.

220 ma marginale, a highly antigenically variant rickettsial pathogen, and creates strong selective press

221 Anaplasma marginale, a rickettsial pathogen, evades clearance in the animal hos

222 he family Bunyaviridae and an emerging human rickettsial pathogen, Rickettsia philipii, in a populati

223 This bacterium is an obligate intracellular rickettsial pathogen.

224 ogous system from an obligate intracellular (rickettsial) pathogen.

225 e proteins, Sca2 may play a critical role in rickettsial pathogenesis.

226 a better understanding of the mechanisms of rickettsial pathogenesis.

227 vides insight on the origin of mechanisms of rickettsial pathogenicity.

228 d will provide insight into the mechanism of Rickettsial pathogenicity.

229 ve demonstrated the transmissibility of both rickettsial pathogens and novel Rickettsia species or st

230 Arthropod-borne rickettsial pathogens cause mild and severe human diseas

231 dynamics of this bacterium and perhaps other rickettsial pathogens from medically important vectors.

232 Rickettsial pathogens in the genera Anaplasma and Ehrlic

233 id mite larvae) are best known as vectors of rickettsial pathogens, Orientia spp., which cause a zoon

234 The pso operon is conserved among rickettsial pathogens, suggesting that bactericidal anti

235 plicable to other obligates, particularly to rickettsial pathogens, to routinely perform targeted mut

236 and tested against a panel of ehrlichial and rickettsial pathogens.

237 This rickettsial pathway compensates for the evolutionary los

238 Endothelium was the major site of rickettsial persistence, including sites in the vital or

239 The mechanism underlying rickettsial phagosomal escape remains unknown, although

240 Rickettsial PKMT1 and PKMT2 are unusual in that their pr

241 with the fast-growing barbed end towards the rickettsial pole.

242 Since AdoMet is required for rickettsial processes, the apparent inability of this st

243 rom lethal rickettsial challenge by limiting rickettsial proliferation in vivo, whereas partial prote

244 proteins (including cytokines), and 1 was a rickettsial protein, the putative N-acetylmuramoyl-l-ala

245 comparatively little is known regarding the rickettsial proteins involved in its organization.

246 represents a mechanism for the secretion of rickettsial proteins, including virulence factors, into

247 Despite the genetic intractability of Rickettsiales, recent advancements have been made in the

248 ong Rickettsia species (Alphaproteobacteria: Rickettsiales), REIS has the largest genome sequenced to

249 poson mutagenesis provides a useful tool for rickettsial research.

250 s 20/2000 and the patient was diagnosed with rickettsial retinitis along the superotemporal retinal v

251 Patients with rickettsial retinitis may develop a sea-fan retinal neov

252 sm, we identified a specific mutation in the rickettsial rpoB gene that confers resistance to rifampi

253 ng the three mutations in the Rifr region of rickettsial rpoB.

254 To investigate whether transcription of the rickettsial rrs responds to amino acid starvation condit

255 known about the function of other conserved rickettsial Sca proteins.

256 we found VP of Mycoplasma, Rhizobiales, and Rickettsiales showed significantly higher counts of (AG)

257 cinia, and varicella-zoster viruses; and two rickettsial species at concentrations mostly ranging fro

258 extrachromosomal DNA element in a pathogenic rickettsial species does not affect either in vitro prol

259 n, although the genomic sequences of several rickettsial species have allowed for the identification

260 previous bioinformatic analysis of sequenced rickettsial species identified a family of at least 17 p

261 Microscopy demonstrated the presence of both rickettsial species in tick salivary glands, and immunoh

262 lysis of eight completed genome sequences of rickettsial species revealed a high degree of sequence c

263 vectors are capable of transmitting several rickettsial species to vertebrate hosts, resulting in va

264 T. gondii and five rickettsial species were recorded in ticks collected fro

265 uter membrane protein B (OmpB) occurs in all rickettsial species, serves as a protective envelope, me

266 ents with high titers of antibodies to other rickettsial species.

267 be a sensitive and specific marker for acute rickettsial spotted rickettsioses.

268 murine bone marrow-derived DCs (BMDCs) after rickettsial stimulation in vitro and their protective ro

269 lied successfully to the characterization of rickettsial stock cultures, the replication of rickettsi

270 additional function for the highly conserved rickettsial surface cell antigen, rOmpB, and suggest tha

271 These processes require the interaction of rickettsial surface proteins with mammalian host cell re

272 hat recognize conformational epitopes on the rickettsial surface.

273 t Sca2 may play an important function at the rickettsial surface.

274 re on the SPA surface but not exposed on the rickettsial surface.

275 sphatase may function as a buffer, enhancing rickettsial survival within the cytoplasm of a eukaryoti

276 ogenic and immune pathways and mechanisms of rickettsial survival within the vertebrate host and tick

277 e examined the rompA gene of a nonpathogenic rickettsial symbiont isolated from the tick Ixodes scapu

278 However, rickettsial symbionts in these vectors are underexplored

279 Maternally inherited rickettsial symbionts of the genus Wolbachia occur commo

280 However, rickettsial tails lacked ezrin, paxillin, and tropomyosi

281 ated with the development of immunity, their rickettsial target and contribution to disease pathogene

282 ehrlichial pathogen of cattle, in the order Rickettsiales, that establishes persistent cyclic ricket

283 ollum-Pratt Institute combined with study of rickettsial toxin at Maryland.

284 The availability of a positive selection for rickettsial transformants is an important step in the ch

285 n is critical to a thorough understanding of rickettsial transmission in tick populations and the eco

286 e a novel biochemical pathway that relies on rickettsial transport of host cytosolic dihydroxyacetone

287 Rickettsial transport systems for substrates found only

288 The paradigm for the study of rickettsial transport systems is the ATP/ADP translocase

289 The existence of a rickettsial transporter for AdoMet raises intriguing que

290 tsiae as well as its potential to be used in rickettsial transposon-based mutagenesis.

291 These data suggest the existence of multiple rickettsial triose phosphate transport systems.

292 this discrepancy to convergence of SAR11 and Rickettsiales tRNA base compositions.

293 he most relevant host cell type, in light of rickettsial tropism for microvascular endothelium in viv

294 l vir T4SS of Agrobacterium tumefaciens, the Rickettsiales vir homolog (rvh) T4SS is characterized pr

295 Little is known about specific Rickettsial virulence factors and their mode of pathogen

296 aracterization and definition of its role in rickettsial virulence.

297 vering the link between OmpB methylation and rickettsial virulence.

298 Herein, Rickettsiales were identified by PCR in five species of

299 ver event resulted in the replacement of the rickettsial wild-type gene with a partially deleted pld

300 hrough heterologous localization assays with rickettsial ZitP and PopZ orthologs, we document the sha