ライフサイエンスコーパス: second dimension

1 00 (110 in the first dimension and 38 in the second dimension).

2 ography at critical conditions (LCCC) in the second dimension.

3 pillaries that provide the separation in the second dimension.

4 mension and capillary electrophoresis as the second dimension.

5 refocusing of (organic) solutes prior to the second dimension.

6 nd loss of resolution and sensitivity in the second dimension.

7 the organic fraction from the analyte in the second dimension.

8 n and spreads overlapping signal across this second dimension.

9 vision, or a separation of flow paths in the second dimension.

10 or 100 muL loops and later discharged to the second dimension.

11 pillary column low-pH RPLC separation in the second dimension.

12 raphic dimension for separation later in the second dimension.

13 ns during the limited separation time in the second dimension.

14 tal water regulation strategy, loads along a second dimension.

15 on scheme in which the ion size appears as a second dimension.

16 rap columns and subsequently analyzed in the second dimension.

17 dentified and quantified after separation in second dimension.

18 e first dimension; only six co-eluted in the second dimension.

19 rform sensitive detection of weak acids in a second dimension.

20 ses (at high flow rates) are required in the second dimension.

21 lumn is analyzed at least three times in the second dimension.

22 um NSP-35 nanostationary phase column in the second dimension.

23 reversed phase separation in the orthogonal second dimension.

24 1)D) column for further resolution on a long second dimension ((2)D(L)) column for parallel detection

25 sion ((1)D) with a multimethod option in the second dimension ((2)D) (choice between size exclusion (

26 on ((1)D) and multiple chiral columns in the second dimension ((2)D) (teicoplanin, teicoplanin aglyco

27 ensions, that is, first dimension ((1)D) and second dimension ((2)D) are treated separately, and the

28 ns were then modulated and separated using a second dimension ((2)D) column via GC x GC, resolving th

29 The second dimension ((2)D) column was resistively heated an

30 until their sequential reinjection onto the second dimension ((2)D) column.

31 oftware is to accurately model the effect of second dimension ((2)D) injection band broadening under

32 ave focused on building retention models for second dimension ((2)D) separations involving variables

33 with different separation properties in the second dimension ((2)D) that can be selected during the

34 ployed multiple channels (4 channels) in the second dimension ((2)D) to increase the (2)D separation

35 e (C18, amide, cyano, phenyl and PFP) in the second dimension ((2)D) were combined to obtain the maxi

36 such methods are applied orthogonally to the second dimension ((2)D), background correction is dramat

37 hase liquid chromatography (LPH-RPLC) in the second dimension ((2)D).

38 n profile similarity in the first ((1)D) and second dimension ((2)D).

39 ((1)D), which lead to peak broadening in the second dimension ((2)D).

40 simple alkane mixture using the RTIL-coated second-dimension ((2)D) mucolumn produced reasonably goo

41 ple heart-cutting valve prior to transfer to second-dimension ((2)D) neonatal crystallizable fragment

42 GC-ToF-MS) with a particular emphasis on the second-dimension ((2)D) retention time predictions ((2)t

43 The second-dimension ((2)D) temperature programming system (

44 y one or more detectors at the outlet of the second dimension, (2)D, with very short runs to avoid un

45 s onto a momentum coordinate in a conceptual second dimension(24-34).

46 In the second dimension, 30 s gradients at a cycle time of 1 mi

47 In the second dimension, a neutral-coated capillary is used for

48 The second dimension accounted for phylogenetic change towar

49 e LC in the first dimension with TRLC in the second dimension, achieves baseline separation of otherw

50 protein A-modified C-CP fiber column in the second dimension, all in a 10 min workflow.

51 The use of the second dimension allowed for successful fragment assignm

52 the first and a reversed phase column in the second dimension allows for a detailed sample evaluation

53 ization mass spectrometry (MALDI-MS), as the second dimension, allows precise determination of the ma

54 Second-dimension analyses are performed and completed ev

55 the spatial domain ((x)LC) in the first and second dimension and time domain ((t)LC) for the third d

56 ray of parallel channels were tested for the second dimension, and improved performance was observed

57 using ultrafast chiral chromatography in the second dimension are successfully applied to the separat

58 en the solvent systems used in the first and second dimensions as a major obstacle.

59 th conventionally sized columns in the rapid second dimension, as solvent consumption is drastically

60 first-dimensional column and transfer to the second dimension before MS or MS/MS analyses.

61 ontaining fractions are then analyzed in the second dimension by either MALDI-PSD or nano-ES with pre

62 ons are transferred across an interface to 5 second-dimension capillaries, and analyte is detected by

63 cally transferred across an interface into a second dimension capillary, where components are further

64 The second-dimension capillary contains an SDS buffer for mi

65 ides were rapidly sampled into a 1.3-cm-long second-dimension channel, where they were separated by c

66 orthogonally intersected a high-aspect ratio second-dimension channel.

67 In a second dimension chromatography seleno-amino acids were

68 oose virtually any combination of first- and second-dimension column diameters without loss in system

69 sing high-polarity first-dimension and polar second-dimension column phases, with a 4 s modulation pe

70 and the very steep gradients utilized in the second-dimension column succeeded in overcoming pH and o

71 lumn that is typically narrow and long and a second-dimension column that is wide and short.

72 was successfully designed and employed as a second-dimension column using comprehensive two-dimensio

73 illary with a PDMS stationary phase, and the second-dimension column was a 0.5 m long, 100 mum i.d. c

74 The effluent exiting the second-dimension column was in the range 6-8 mL/min, wit

75 as chromatography mass spectrometry with the second-dimension column working under low-pressure condi

76 Analytes were separated on the second-dimension column, (2)D, with (2) w(b) ranging fro

77 in eluent flow rate and column length of the second-dimension column, and (5) the maximum achievable

78 y is coupled to a 0.075 mm internal diameter second-dimension column.

79 at is, with three to four separations on the second dimension (column 2) per peak width from the firs

80 capacities can be achieved by using shorter second-dimension columns and collecting a relatively lar

81 l liquid chromatography that makes the rapid second dimension compatible with mass spectrometry witho

82 (LC x LC), few approaches exist whereby the second dimension comprises the chiral separation.

83 The second dimension consisted of a standard reversed-phase

84 iltration column was then transferred to the second dimension consisting of a monolithic C-18 column

85 The second dimension consists of a 1.2 cm x 1.2 cm mucolumn

86 The second dimension consists of discontinuous SDS-PAGE.

87 ted fractions were then characterized by the second dimension (D2) size exclusion chromatography (SEC

88 m (phase of the circadian rhythm), while the second dimension - distinctness (subjective amplitude) h

89 on to a range suitable for microinjection by second dimension electrophoresis and enzymatic digestion

90 onged time periods necessary to complete the second-dimension electrophoretic separation step--denatu

91 velocity and initial organic content of the second-dimension eluent.

92 -CGE) were repetitively transferred into the second dimension every 0.5 s of run time in the first di

93 dimension was repetitively injected into the second dimension every few seconds.

94 trated abundant fragment ions and provided a second-dimension "fingerprint" of the complex cellular f

95 use of ultrafast chiral chromatography as a second dimension for 2D chromatographic separations.

96 e ( approximately nanosecond) signals adds a second dimension for multiplexing and also permits detec

97 ichment, reversed phase nanoLC column in the second dimension for separation, a benchtop Orbitrap mas

98 time of analytes, were transferred into the second dimension for the further separation and successi

99 pidly sampling and analyzing effluent in the second dimension from the first dimension.

100 In the second dimension, functionalized monolithic columns are

101 sequence, due to the time restriction of the second-dimension gradient time, online 2D-LC schemes can

102 was used to optimize an assembly of shifting second-dimension gradients, which resulted in a high deg

103 iquid chromatography (RPLC) detection in the second dimension (heart-cutting two-dimensional (2D)-HIC

104 ercritical fluid chromatography (SFC) as the second dimension hyphenated to Fourier transform ion cyc

105 y using nonporous reversed-phase HPLC in the second dimension (IEF-NP RP HPLC).

106 se of chiral stationary phases (CSPs) as the second dimension in 2D-LC, especially in the comprehensi

107 design of gradient elution strategies in the second dimension in combination with photodiode array de

108 ins, linking the analysis with a MALDI-based second dimension in m/z is shown to be an efficient meth

109 in terms of the first dimension flow rate or second dimension injected volume.

110 flow rate, to reduce gradient time, and low second-dimension injected volume, to limit injection eff

111 The second dimension is based on increasing flow rate gradie

112 c of the protein while migration time in the second dimension is characteristic of the peptide.

113 a compressible sample from the first to the second dimension is demonstrated to yield excellent perf

114 A key advantage of employing UHPSFC in the second dimension is its ability to perform ultrafast ana

115 The second-dimension is a wide-bore column (5 m x 0.53 mm I.

116 composition of the mobile phase used in the second dimension, its initial organic content if this se

117 we show how extending pi-conjugation in the second dimension leads to novel materials with HOMO-LUMO

118 h fraction was then fully transferred to the second-dimension low-pH nanoLC separation using an autos

119 introduce our fluorescent probes including a second dimension: lysosomal pH, since de-acidification i

120 the relatively high flow rates used for the second dimension make direct (splitless) hyphenation to

121 (nLC) separation is coupled directly with a second dimension micro free flow electrophoresis (muFFE)

122 lysis strategy is introduced to leverage the second dimension of 2D MS/MS spectra, in which stairstep

123 ing as a limitation when incorporated as the second dimension of a 2D separation.

124 F or NEPHGE tube gel before using it for the second dimension of a two-dimensional gel.

125 ously developed HX-MS(2) technology to add a second dimension of deuteration data and promote automat

126 The second dimension of difficulty concerns the intermodel d

127 Temperature programming in the second dimension of GC x GC was able to improve separati

128 then released after a delay to initiate the second dimension of IM.

129 ns are then determined from separations in a second dimension of IM.

130 olated as spots off the diagonal line in the second dimension of PAGE.

131 y of pH-selective Fc activation to provide a second dimension of selective tumor cell targeting.

132 111 capillary column is enhanced by adding a second dimension of separation ((2)D) in a GC x GC desig

133 electrophoresis is implemented to provide a second dimension of separation.

134 ed as the separation modes for the first and second dimension of the electrophoresis, respectively.

135 ut enantiopurity analysis and its use in the second dimension of two-dimensional liquid chromatograph

136 el holo/apo conversion between the first and second dimensions of PAGE, holo-metalloproteins in the o

137 SEC at ultrahigh-pressure conditions in the second dimension offered very fast, yet efficient separa

138 The use of SFC in the second dimension offers a wide choice of mobile and stat

139 id points simultaneously along the first and second dimensions on the basis of applying a one-dimensi

140 ed according to the product of the first and second dimension peak capacities.

141 k widths, leading to significantly increased second dimension peak capacity.

142 city is simply the product of the first- and second-dimension peak capacities.

143 observed when the ratio of the first- to the second-dimension peak capacity is much less than unity.

144 parations made with online 2D-LC require the second-dimension peaks to be very narrow, (2) the separa

145 tive mapping will extend that inference to a second dimension representing index species of the 20 li

146 e/interference peak height ratio, first- and second-dimension resolutions, signal-to noise ratio, inj

147 This gives access to calculated second dimension retention indices ((2)I).

148 impact of chromatographic variability on the second dimension retention time, a concept based upon hy

149 on retention time ((1)D RT), followed by the second-dimension retention time ((2)D RT), where the (2)

150 information about certain parameters (e.g., second-dimension retention time variability, first-dimen

151 l complications are observed when first- and second-dimension retention times show some correlation,

152 eparation of individual molecular species by second-dimension reverse-phase HPLC and characterization

153 of these peptides in the flow-through by the second-dimension RP trap can dramatically reduce the com

154 roduction by allowing the use of the longest second dimension run time, while maintaining quantitativ

155 transferred by the same isocratic pump to a second-dimension sample loop.

156 If second dimension SDS-PAGE followed BNP, the 180-kDa muta

157 A proteomic analysis was performed using second dimension SDS-PAGE followed by nano LC-MS/MS.

158 y of P13 within the complex was confirmed by second dimension SDS-PAGE, Western blotting, mass spectr

159 The second dimension, separating auditory-motor and visual p

160 es using an independent pump setup; and 3) a second dimension separation ((2) D-UV-MS) with fully deu

161 separation, relative retention time for the second dimension separation (2DrelRT) and boiling point

162 us sub-3-mum stationary phase provide a fast second dimension separation and a sufficient sampling fr

163 separations are limited by the speed of the second dimension separation and the consequent loss of p

164 k capacity lost due to under sampling by the second dimension separation as peaks elute off the first

165 at the base, (1)w(b) of ~3 s and varying the second dimension separation run time from 300 to 2900 ms

166 ations are used, instead of simply using the second-dimension separation as a desalting step.

167 mpled in parallel by 20 channels effecting a second-dimension separation by native electrophoresis.

168 trating the great potential of the chip as a second-dimension separation column.

169 comprising silicon-micromachined first- and second-dimension separation columns and a silicon-microm

170 o-run variation by including 7 M urea in the second-dimension separation matrix.

171 it possible to quantify diastereomers in the second-dimension separation.

172 formation products was then subjected to the second-dimension separation.

173 o sample the (1)D with an adequate number of second dimension separations.

174 Over 100 transfers and second-dimension separations are performed over an appro

175 apacity and resolution when high-performance second-dimension separations are used, instead of simply

176 High field strength (+2 kV/cm) enables rapid second-dimension separations in which each peak eluted f

177 lex might be specific for leaf growth in the second dimension, since it is not present in Poaceae (gr

178 sence of sodium dodecyl sulfate (SDS) in the second-dimension sizing separation limits the orthogonal

179 ectrophoresis can be conveniently coupled to second-dimension sodium dodecyl sulfate-polyacrylamide g

180 ved in gel shift assays has been resolved by second-dimension sodium dodecyl sulfate-polyacrylamide g

181 n n-octane and trans-2-octene when used as a second dimension stationary phase.

182 Commonly applied SEC separations in the second dimension take several minutes, so that a total L

183 A second-dimension temperature programming system ((2)DTPS

184 ks are compounds with retention times in the second dimension that are longer than the modulation per

185 mmunities increasingly differentiate along a second dimension that reflects a trade-off between incre

186 hy separating according to molar mass in the second dimension, thus providing comprehensive informati

187 The addition of a second dimension to detection probes permits the use of

188 n and an ultrahigh-pressure LC system in the second dimension to ensure maximum sensitivity and perfo

189 quid chromatography (RP-UHPLC) column in the second dimension to further separate the ester species.

190 e size-exclusion chromatography (SEC) in the second dimension to separate the constituting polymer mo

191 e liquid chromatography (RPLC) was used as a second dimension to separate the released dyes.

192 As the second dimension to the genome, the epigenome contains k

193 t restriction endonucleases in the first and second dimensions, to generate filters suitable for imag

194 ous and 2.7 mum fused-core particles) in the second dimension, together with the use of 0.1% phosphor

195 tionary phase (ACQUITY Torus DEA), while the second dimension used a stereoselective polysaccharide s

196 Discrete peaks in the second dimension using the thermal modulator were 30-55%

197 ritical fluid chromatography (UHPSFC) in the second dimension, using a 10 mm long silica column, both

198 zed by a combination of 12 conditions in the second dimension, using four columns with octadecyl, phe

199 In the second dimension, various substituent groups were introd

200 Next, protein transfer to the second dimension was accomplished by chemical mobilizati

201 This second dimension was capable of analyzing all fluid volu

202 imension and the same RP-UHPLC method in the second dimension was developed to analyze the degradatio

203 GC method in which the elution order in the second dimension was highly dependent on the number of d

204 The second dimension was related to an inverse association b

205 etween angiosperms and gymnosperms), and the second dimension was related to changes in maximum tree

206 inute gradient separation of proteins in the second dimension was successful to minimize analysis tim

207 To overcome this problem, the second dimension was temperature-programmed by resistive

208 ntation of such rapid chiral analyses in the second dimension was, thus far, limited by the challengi

209 could be introduced into both the first and second dimensions was also illustrated.

210 multiple ionic-polymer layers CZE-MS in the second dimension, we evaluated our setup by analyzing a

211 this classic problem through extension to a second dimension; we present statistical analysis, Monte

212 ete transfer of a peak from the first to the second dimension, whatever the composition of the mobile

213 eriments, population activity varied along a second dimension, which we refer to as network gain, esp

214 r of the first dimension mobile phase to the second dimension while still achieving chiral separation

215 By coupling the second dimension with multiangle light scattering (MALS)

216 e first dimension and charge variants in the second dimension without any need for manual fractionati