ライフサイエンスコーパス: segregant

1 susceptible phenotypes, were pooled (bulked segregant).

2 istinct genes in 169 yeast cross progeny (or segregants).

3 red on marker D6S1045 at 6q14.3-q15, in 6/19 segregants.

4 production of respiratory-deficient (petite) segregants.

5 r was monitored by selection for TK-positive segregants.

6 tance (ApR); recombination generates Lac-ApS segregants.

7 t carries it by killing plasmid-free (cured) segregants.

8 se perturbation has different effects across segregants.

9 icrog, and 27.9%, respectively, for isogenic segregants.

10 t for a group of genetically different yeast segregants.

11 nctions intracellularly to kill plasmid-free segregants.

12 mapping traits in yeast by genotyping pooled segregants.

13 sence of the PHO1 uORF in a population of F2 segregants.

14 on contained in tetrads as opposed to single segregants.

15 The yofi F2 segregants accumulated prolycopene independently of the

16 er was found through a combination of bulked segregant analysis (BSA) and RAPD techniques.

17 To this end, we applied a bulked segregant analysis (BSA) approach, using whole-genome de

18 ons in the costs of these techniques, bulked segregant analysis (BSA) has become not only a powerful

19 Bulked segregant analysis (BSA) in combination with AFFYMETRIX

20 Bulked segregant analysis (BSA) is an efficient method to rapid

21 Bulked segregant analysis (BSA) is used to identify existing or

22 Bulk segregant analysis (BSA) of an F(2) population from a cr

23 sequences of extra gene copies, we used bulk segregant analysis (BSA) of radiation hybrid (RH) cells.

24 r associative transcriptomics (AT), and bulk segregant analysis (BSA) on DNA pools created from a cro

25 suitable screening method, followed by bulk segregant analysis (BSA) to identify large-effect QTLs.

26 ional QTL analysis allied to a combined bulk-segregant analysis (BSA) using a novel potato whole-exom

27 A bulk segregant analysis (BSA) version of double-digest restri

28 Bulked segregant analysis (BSA), coupled with next-generation s

29 We used bulked segregant analysis and association analysis, genetic met

30 he GA biosynthetic pathway, through a bulked segregant analysis and bioinformatic pipeline, and confi

31 Bulked segregant analysis and candidate gene sequencing reveale

32 Bulked segregant analysis and candidate gene sequencing reveale

33 ntification of SMO1 by a combination of bulk segregant analysis and comparative genome analysis.

34 nicle1 (spp1) phenotype, we performed bulked segregant analysis and deep sequencing to fine map it to

35 Bulk segregant analysis and full-genome sequencing identified

36 sly applied to Chlamydomonas, such as bulked segregant analysis and marker duplexing, are being imple

37 Bulked segregant analysis and sequencing of resistant and susce

38 Through bulk segregant analysis and transgenic experiment, we show th

39 loral mutant of Mimulus lewisii through bulk segregant analysis and transgenic experiments and identi

40 duced mutant of Mimulus lewisii through bulk segregant analysis and transgenic experiments, we have i

41 ned the EGT2 gene by a combination of bulked-segregant analysis and whole genome sequencing.

42 We used a bulk segregant analysis approach and genotyped the extreme cl

43 We employed a bulked segregant analysis approach using historical data from t

44 entify the causal gene(s), we applied bulked segregant analysis by whole genome sequencing.

45 t the power of sequencing combined with bulk segregant analysis can also be applied to a nongenetical

46 By analyzing evolved cells using bulk segregant analysis coupled with whole-genome sequencing,

47 he applicability of this map, we used bulked segregant analysis followed by interval mapping to locat

48 e tiling arrays and microarray-assisted bulk segregant analysis followed by linkage mapping.

49 Bulk segregant analysis followed by whole genome resequencing

50 a tetraploid intermediate, followed by bulk segregant analysis in conjunction with high-throughput s

51 Bulked segregant analysis in SC x SI F2 individuals using deep

52 ponsible, we performed flow sorting and bulk segregant analysis of 25 proteins, finding a median of f

53 genes, we applied fine mapping through bulk segregant analysis of near-isogenic progeny with distinc

54 isolates of different virulence and use bulk segregant analysis of whole-genome sequences from the pr

55 A phenotypic bulked segregant analysis revealed that bulks representing the

56 Bulked segregant analysis revealed two DNA markers (28-178 and

57 This strategy uses masking bulk segregant analysis to mask unrelated deletions, thus all

58 rray hybridization can be combined with bulk segregant analysis to quickly map mutations.

59 Here, AFLP bulk segregant analysis using H. melpomene crosses identified

60 Bulk segregant analysis using next-generation sequencing reve

61 To genetically map LrAp, bulked segregant analysis was combined with resistance gene enr

62 fragment length polymorphism (AFLP) and bulk segregant analysis were used to map the Def-1 gene to a

63 We genetically mapped the mutations by bulk segregant analysis with high-density oligonucleotide arr

64 nt progeny for genetic linkage mapping, bulk segregant analysis, and high-throughput 'omics readouts.

65 ree lines using deep sequencing-based bulked segregant analysis, and in one case confirmed by transge

66 This study combined bulked-segregant analysis, array comparative genomic hybridizat

67 Proctor and subsequent SNP array-based bulk segregant analysis, fine mapped the mutation to a cM sca

68 trate a new method, microarray-assisted bulk segregant analysis, for mapping traits in yeast by genot

69 idely used mapping techniques like F(2) bulk-segregant analysis, our method produces near-isogenic li

70 Applying bulk segregant analysis, quantitative trait loci mapping usin

71 By combining this technique with bulk segregant analysis, several high heritability developmen

72 Using bulk segregant analysis, we have focused our mapping efforts

73 Using bulked segregant analysis, we have identified amplified fragmen

74 Using bulk segregant analysis, we identified a high-confidence Quan

75 a bi-parental mapping population and bulked segregant analysis, we identified Autoflower2, a 0.5 Mbp

76 Using our first-pass marker panel in bulked-segregant analysis, we were able to identify the genetic

77 errestris was placed on the map using bulked segregant analysis.

78 s were identified as linked to Ctv by bulked segregant analysis.

79 2 (R) and Butter Bush (S) using QTL-seq bulk segregant analysis.

80 etically map mutants via quantitative bulked segregant analysis.

81 his major gene using the technique of bulked segregant analysis.

82 ntegrated, targeted approach based on bulked segregant and differential display analysis.

83 the dry grain, was degraded in both the null segregant and homozygote after imbibition.

84 ltimer resolution, lethality to plasmid-free segregants and active partitioning functions.

85 les seed sorting between transgenic and null segregants and are ideal for comparative analysis.

86 olymorphisms at an error rate close to 3% in segregants and at an error rate of 7% in diploid strains

87 by crossing those strains, phenotyping 1500 segregants, and genotyping of high-survival segregants b

88 ss, the mean effect of a perturbation across segregants, and interacting loci.

89 encodes Doc, a toxin that kills plasmid-free segregants, and Phd, an unstable antidote that neutraliz

90 Here we use a bulk-segregant approach to identify the beneficial mutations

91 Major classes of segregants are those carrying homozygous insertion mutat

92 1-HSV-2 hybrid tk sequences gave rise to tk+ segregants at an average rate of 10(-8) events per cell

93 ted with fitness variation inferred from the segregant bulk fitness assay.

94 ations of 10-100 million haploid and diploid segregants by crossing two budding yeast strains of diff

95 segregants, and genotyping of high-survival segregants by hybridization of bulk and single segregant

96 (20%) of these giving rise to transformation segregants containing exclusively the initially nonselec

97 Segregants containing the YGL001c disruption were not vi

98 Segregants containing the YLR100w disruption failed to g

99 g raffinose, was greater than that from null segregant controls and this phenomenon was partially res

100 Relative to null segregant controls lacking the transgene, homozygotes ov

101 antly reduced seed vigor, compared with null segregant controls.

102 (e) breast cancer data, as well as (f) yeast segregant data to validate the ability of the proposed m

103 ome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5)

104 alidation by WGS, we sequenced haploid yeast segregants derived from a popular commercial mutant coll

105 ng data from 18,233 yeast cells from 4489 F2 segregants derived from an F1 cross between the laborato

106 -type (WT; non-mutagenized) genotype, and F2 segregants displaying the same phenotype are subsequentl

107 gregants by hybridization of bulk and single segregant DNA to microarrays.

108 to show variable effects, including baseline segregant fitness, the mean effect of a perturbation acr

109 were then analyzed for linkage using meiotic segregants; four linkage groups were identified in chrom

110 mes, a panel of Round II (genomic exclusion) segregants from a B/C3 heterozygote was used.

111 Haploid segregants from a cross between a common laboratory stra

112 rallel genotype and gene expression data for segregants from a cross between two strains of the yeast

113 Here, we show that in segregants from a cross between two unrelated strains of

114 ces systematically, we treated 104 genotyped segregants from a cross between two yeast strains with a

115 ated the LIR, as compared to 3% of the tk(+) segregants from LDR cell lines, corresponding to a >20-f

116 i-complementation mutants and transgene-null segregants from RNAi suppression lines to sub-compartmen

117 Unexpectedly, a single rare fut1(-) segregant ( fut1(s) ) was obtained in rich media, which

118 of RAD markers for both individual and bulk-segregant genotyping.

119 By sequencing pooled DNA from millions of segregants grown under heat stress, we further identifie

120 on with next-generation sequencing on bulked segregants in the same accession using sequence polymorp

121 r 'cloning by sequencing': one based on bulk segregant linkage (BSFseq) and one based on homozygosity

122 Bulked-segregant mapping combined with pooled sequencing provid

123 We then developed a population-level bulk segregant mapping method, based on high-throughput genom

124 One meiotic segregant mated poorly with the serotype A reference str

125 sis of the introduced chromosome in immortal segregants narrowed the candidate interval to 2.7 Mb spa

126 ility in CE-1 white flour relative to a null segregant (NS) control.

127 ger seedling shoots and roots, than the null segregant (NS) controls.

128 sequencing in pheromone-treated cells of 43 segregants of a cross between two highly diverged yeast

129 Using pooled F12 generation segregants of different hybrids with extreme phenotype d

130 Full segregants of the sll0254 deletion in Synechocystis were

131 number (one to two copies) in some of the F2 segregants, perhaps resulting from the clustering of PCP

132 We then subjected these large segregant pools to heat stress for up to 12 d, enriching

133 analysis of PMN responses to O3 exposure in segregant populations derived from inflammation-prone (s

134 fected with the various substrates and tk(+) segregants produced via intrachromosomal recombination w

135 ion in the resistant F2 and backcross-bulked segregant progeny.

136 chromosome complement of individual plants (segregants) ranged from 36 to 42, with a bias toward the

137 To explain the high incidence of 3:1 segregants, rarely seen in offspring of carriers of othe

138 Of the tk(+) segregants recovered from LIR-containing cell lines, 14%

139 With RNA-sequencing analysis of bulked segregants representing the tails of a population segreg

140 stant (n = 20) and susceptible (n = 20) bulk segregants revealed ~900,000 single nucleotide polymorph

141 We applied a two-step bulked segregant RNA sequencing (BSR-Seq) approach in developin

142 Here, we used bulked segregant RNA sequencing and map-based cloning to identi

143 Here we used crossing studies, bulk-segregant RNA sequencing, phylogenetic analyses and func

144 The mutant loci were isolated by bulked segregant RNA sequencing.

145 Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identif

146 Bulked segregant RNA-seq analysis and positional cloning reveal

147 nd wildtype color phenotypes and used bulked segregant RNA-Seq to identify a region on chromosome 6 t

148 B1 was identified using bulked segregant RNA-sequencing of an F(2) durum wheat populati

149 mplement to the QTL mapping approach, bulked segregant RNAseq analysis revealed a small number of can

150 formed seed compared with 10.9%TFA in a null segregant seed and 53.2%TFA in the current best source o

151 a 110- and 7-fold improvement over the null segregant seed and linseed oil, respectively.

152 HERIPIC allows users to rapidly analyse bulk segregant sequence data and we have made it available as

153 CHERIPIC using three different types of bulk segregant sequence data from Arabidopsis, maize and barl

154 nstructed trilayer-like superstructures with segregant-specific compositional profiles that facilitat

155 gene-expression levels in 95 genotyped yeast segregants subjected to a drug perturbation.

156 accessions, and identified two transgressive segregants that are susceptible to the pathogen.

157 restored in later generations, even in those segregants that inherited the corresponding parental rDN

158 n and methylation are not heritable: meiotic segregants that lack Ufo1 revert to the normal P1-wr exp

159 However, transgressive segregants that outperformed the original elite hybrid v

160 GRP)-4X mapping population and sequenced 192 segregants to generate an accurate genetic map.

161 Deletion analysis of immortal segregants using polymorphic markers revealed the loss o

162 However, one segregant was able to grow, and genetic analysis indicat

163 This meiotic segregant was used to create congenic a and alpha mating

164 quency of disease in the B lymphocyte intact segregants was equivalent to that of standard NOD mice i

165 In this line and its null segregant we analyzed GSP accumulation and expression of

166 1c ORF was made in a diploid strain, and the segregants were plated onto sterol supplemented media un

167 Hybrid segregants were prepared from the albumin-extinguished h

168 cated near the ZEP/ABA1 gene, but the bulked segregant whole genome sequencing approach more efficien

169 single transformation, allows selection for segregants with two copies of the insertion.