ライフサイエンスコーパス: selectable

1 lection, while epistatic effects will not be selectable.

2 structure and at least 7.0 kb in order to be selectable.

3 8, although other nearby cleavage sites were selectable.

4 y making regulatory mutations phenotypically selectable.

5 ulfills both functions: it is a conventional selectable aadA gene in culture, and allows detection of

6 when paired with the TCRbeta chain, forms a selectable alphabeta TCR.

7 ot encode TCRalpha chains capable of forming selectable alphabeta TCRs can be excised from the chromo

8 Expression cassettes comprise selectable and FACS-sortable markers in tandem with addi

9 n emphasizing such non-genomic mechanisms of selectable and heritable variation, Eco-Evo-Devo present

10 Our results demonstrate selectable and near-single-dose production of these biol

11 ated hematopoietic chimeras with mixtures of selectable and nonselectable precursors.

12 crowave photonic (MWP) multiband filter with selectable and switchable passbands is proposed and expe

13 es are replaced with a cassette containing a selectable antibiotic resistance and oriT(RK2) for effic

14 n of transformed genomes is achieved using a selectable antibiotic resistance marker that has no subs

15 tion in murine ES cells, heterozygous at the selectable Aprt locus, differs from that in embryonic so

16 e of a novel phenomenon, we have developed a selectable assay to detect contractions of CTG/CAG tripl

17 We constructed DNA vectors that are selectable based on their copy number and used these to

18 that each N-oxide reductase could provide a selectable benefit on its own, and therefore, reduction

19 We describe the development of a selectable, bi-cistronic subgenomic replicon for bovine

20 fabR) gene was deleted by replacement with a selectable cassette, and the resulting strains (fabR::ka

21 ettes, essential metabolic genes, negatively selectable catabolic genes and even intact operons from

22 hypermutation machinery and coupling this to selectable cell surface display of a Tie2 ectodomain.

23 new ways of linking desirable phenotypes to selectable characteristics.

24 ly, linear molecular clocks that incorporate selectable characters are in any case problematic.

25 orted here, we developed a system in which a selectable chimeric RNA replicon was transcribed from an

26 tructs and resulted in the introduction of a selectable chloramphenicol resistance marker into the ch

27 romosomes, and the frequency of loss of this selectable chromosomal marker (LOM) in normal mammalian

28 is work describes the novel use of tolC as a selectable/counter-selectable marker for the facile modi

29 nsport in E. coli, has been implemented as a selectable/counter-selectable marker, but counter-select

30 cation for the evolved genes as dual-purpose selectable/counter-selectable markers.

31 tron artificially transplanted into the URA3 selectable/counterselectable auxotrophic marker.

32 s of human-ortholog genes, and provides user-selectable cutoffs and multiple testing correction metho

33 rent pathways may be short-circuited by ATRA-selectable defects at relapse On-ATRA.

34 Additional selectable, dicistronic RNAs encoding NS2-NS5B, E1-NS5B,

35 ectrically deformed either radially or along selectable directions, so as to tune defocus or astigmat

36 irically defined a simple formula for a user-selectable discriminant line that categorizes a peptide

37 phage lambda that are marked with different selectable drug-resistant markers.

38 t the demonstration of high-performance bias-selectable dual-band long-wavelength infrared photodetec

39 This bias-selectable dual-band photodetector is compact, with no m

40 Fluorescence was chosen as the selectable endpoint as fluorescence can be easily detect

41 s with biological utility can be linked to a selectable Fah transposon cassette.

42 ll receptors, every thymocyte is potentially selectable for maturation in the CD4 lineage.

43 rticle detection and radiation generation at selectable frequencies.

44 cquire the rare mutations that provide a new selectable function.

45 omatically be considered proof of the gene's selectable function.

46 as 98 bp are selectable in TSCER2, although selectable gene conversion tracts could include a wide r

47 ransfected with a chimeric RNA composed of a selectable gene flanked by the termini of RNA2, the chim

48 to replacement of the targeted locus with a selectable gene from the targeting DNA.

49 argeted locus creates a mismatch between the selectable gene on the targeting DNA and the targeted ge

50 The dominant selectable gene, Ecogpt, has been introduced, by the cal

51 roplast, allowing to introduce an exogenous, selectable gene.

52 Using selectable genes as proof of principle, a new high-throu

53 Using a selectable genetic system for detection of repeat contra

54 can generate particular organs, can produce selectable genetic variation for the entire animal, can

55 ants containing one or more copies of bar, a selectable herbicide (Basta) resistance gene, located be

56 ells containing the BRCA1 promoter driving a selectable HPRT gene, long-term silencing of the promote

57 y, we generated quasiviruses containing G418-selectable HPV-31 genomes with phosphodeficient phenylal

58 also developed heterologous markers that are selectable in A. nidulans but do not direct integration

59 ast, continuous tracts as short as 98 bp are selectable in TSCER2, although selectable gene conversio

60 ds to increased lambdoid prophage induction (selectable in vivo expression technology assay), suggest

61 To study induced STEC, we developed a selectable in vivo expression technology, SIVET, a repor

62 created Deconvoluter-ordered libraries with selectable insertions every 50 kb in the E. coli genome.

63 o screen for loss of silencing of a dominant selectable kanMX reporter gene embedded within fission y

64 The system incorporates a selectable, large, unnatural amino acid and may ultimate

65 The imaging system described here uses selectable laser wavelengths and synchronized detection

66 roaryl mesylates (Ms) deliver the other in a selectable manner.

67 vitro transposition system to deliver both a selectable marker (kanamycin) and an Escherichia coli pl

68 Non-Mendelian inheritance of a selectable marker (neo), used to identify plastid DNA tr

69 on containing a retrotransposition-activated selectable marker (RAM), which enables one-step bacteria

70 n that is heterozygous for an insertion of a selectable marker (URA3) within this tandem array, and h

71 rtificial chromosome (YAC) cassette (a yeast selectable marker and a centromere).

72 sitive selectable marker, URA3 as a negative selectable marker and a gene-specific sequence called a

73 ement of the entire gene with a heterologous selectable marker and correction of a single base pair i

74 on of lox sites resulting in excision of the selectable marker and cre genes.

75 A selectable marker and EGFP marker were then added by lox

76 sequence from one BAC is cloned alongside a selectable marker and placed between unique sequence arm

77 on of a plasmid-encoded mevinolin resistance selectable marker and plasmid recovery after transformat

78 t facilitate transgene genome integration, a selectable marker and promoter elements driving a coding

79 esults demonstrate the availability of a new selectable marker and shuttle vector for genetically dis

80 rk, we have developed a system to excise the selectable marker and the cre recombinase genes from tra

81 d may be used in combination with a negative selectable marker as part of a two-step selection system

82 iviruses expressing a bicistronic RNA with a selectable marker as the first open reading frame (ORF)

83 ltiple loci, we show that the insertion of a selectable marker at one control site frequently coincid

84 be modified without introducing an unwanted selectable marker at the modification site.

85 loop sequence, therefore, the URA3 negative selectable marker becomes mitotically unstable and is lo

86 Flanking of the selectable marker by loxP sites followed by Cre-mediated

87 where a mini-Tnt1 with a retrotransposition selectable marker can only transpose when Tnt1 proteins

88 s is the first example of FHV RNA1-dependent selectable marker expression initiated in vivo and will

89 Analysis of the selectable marker expression over multiple generations r

90 he cassette consists of the S. pombe ura4(+) selectable marker flanked by a wild-type loxP site at on

91 rgeting frequency for replacement vectors (a selectable marker flanked by homologous DNA).

92 A dominant selectable marker for Candida albicans and other Candida

93 nal resistance gene for its suitability as a selectable marker for chloroplast transformation.

94 ain why blasticidin has become the preferred selectable marker for making gene knockouts.

95 eR (bleomycin resistance) gene as a positive selectable marker for nuclear transformation, an inserti

96 data indicate that CYP76B1 can function as a selectable marker for plant transformation, allowing eff

97 rom Agrobacterium tumefaciens, as a positive selectable marker for plastid transformation.

98 he novel use of tolC as a selectable/counter-selectable marker for the facile modification of DNA in

99 eaminase gene codA as a conditional negative selectable marker for use in the model green alga Chlamy

100 ol of the app1 promoter and, together with a selectable marker gene (hygromycin phosphotransferase),

101 The inactivation of a selectable marker gene adjacent to a telomere as a resul

102 rogeny of co-transformants carrying both the selectable marker gene and the effector transgene.

103 to orthophosphate (Pi) offers an alternative selectable marker gene as demonstrated for tobacco and m

104 ls during RNP delivery, a plasmid carrying a selectable marker gene can be co-delivered with the RNP

105 t, containing genes of the PHB pathway and a selectable marker gene encoding spectinomycin resistance

106 KEY MESSAGE: A new selectable marker gene for stable transformation of the

107 A new selectable marker gene for stable transformation of the

108 ants have either relied on the presence of a selectable marker gene in the insertion cassette or occu

109 Incorporation of a selectable marker gene in the plastid genome is essentia

110 The selectable marker gene neomycin phosphotransferase (NEO)

111 esized nptII gene becomes a highly efficient selectable marker gene that facilitates the selection of

112 quent DNA repair activated expression of the selectable marker gene within the donor locus.

113 ing the production of transgenic plants, the selectable marker gene(s) used in the process are redund

114 y, we have shown that with the use of a drug-selectable marker gene, chemotherapy can select for cell

115 s from sorghum linkage group J, mixed with a selectable marker gene, directly into maize cells using

116 Additionally, the expression of a selectable marker gene, multidrug resistance (MDR) type

117 s conducted thus far have relied on a single selectable marker gene, the spectinomycin resistance gen

118 y the constitutive CsVMV promoter and an HPT selectable marker gene, was also transformed into tobacc

119 leavage sites, a GFP reporter gene and a PAT selectable marker gene, was transformed into tobacco.

120 number of crops include the dearth of proven selectable marker gene-selection combinations and tissue

121 used to introduce the therapeutic gene and a selectable marker gene.

122 together with conventional T-DNAs carrying a selectable marker gene.

123 in KC, by linking its expression to the MDR1 selectable marker gene.

124 ci without the concomitant introduction of a selectable marker gene.

125 , while simultaneously removing the unwanted selectable marker gene.

126 astid transformation vector that harbors two selectable marker genes (aph A-6 and npt II) to detoxify

127 ystem that allowed excision and recycling of selectable marker genes (SMGs).

128 Selectable marker genes are required to ensure the effic

129 igins, cis-acting partitioning elements, and selectable marker genes were constructed and analyzed fo

130 ingle plant, there is a need for alternative selectable marker genes.

131 reptomyces ribosome binding sites, and yeast selectable marker genes.

132 ion procedures required no tissue culture or selectable marker genes.

133 tors containing epitope tags followed by the selectable marker hph (resulting in hygromycin resistanc

134 Cre/loxP system for Neurospora, allowing the selectable marker hph to be excised by introduction of C

135 ction, dispensing with the requirement for a selectable marker in plant transgenesis.

136 Two alleles contain insertions of a selectable marker in the first exon in either orientatio

137 ndogenous to plants, the use of Atwbc19 as a selectable marker in transgenic plants may provide a pra

138 ene, each gene was incorporated as the yeast selectable marker into a set of shuttle vectors designed

139 y inserting an E.coli replication origin and selectable marker into isolated, circular mtDNA at rando

140 system for BAC modification by introducing a selectable marker into the vector sequence of a BAC clon

141 f a construct containing a reporter gene and selectable marker into these microspores and hence, afte

142 implest method used to remove a loxP-flanked selectable marker is to breed an animal carrying a loxP-

143 oietic stem cells (HSCs) and is considered a selectable marker of human HSCs, although its contributi

144 asmid integration, we observed transfer of a selectable marker placed virtually anywhere on the chrom

145 test protein with Tat export efficiency of a selectable marker protein, namely TEM-1 beta-lactamase.

146 expression system, a number of commonly used selectable marker proteins were assessed for their compa

147 Circular DNA containing a selectable marker replicates and is maintained under sel

148 aining the neomycin phosphotransferase (neo) selectable marker resulted in selection of G418-resistan

149 r intron upstream of a reporter gene or drug-selectable marker reveal that the intron also possesses

150 fected using retroviral vectors containing a selectable marker such as neomycin resistance.

151 mutant (Y426M + S305L) was used to develop a selectable marker system for Agrobacterium tumefaciens-m

152 Two of the most popular dominant selectable marker systems for plant transformation are b

153 e copy number distribution relative to other selectable marker systems, but the most tolerant events

154 s in T. denticola and possesses an efficient selectable marker that provides a new tool for treponema

155 (essential for peptidoglycan synthesis) as a selectable marker to complement the lethal chromosomal D

156 nfounded by the fact that LEU2 was used as a selectable marker to create Atm1p-depleted cells, wherea

157 enome editing in S. rosetta by engineering a selectable marker to enrich for edited cells.

158 The selectable marker used in these studies was the CP4 EPSP

159 allow serial replacement by recycling of the selectable marker using Cre-loxP.

160 s deleted by homologous recombination, and a selectable marker was inserted in its place.

161 native-like infection conditions, a neomycin-selectable marker was inserted into L1/L2 of the HPV-31

162 Transfer of this selectable marker was observed to be accompanied by chro

163 n either orientation, and, in the third, the selectable marker was removed, resulting in premature te

164 ley co-transformants involving hpt::gfp (the selectable marker) and gus (a model transgene of interes

165 ective cassette (e.g., fluorescent protein + selectable marker).

166 ted into the TAR vector along with a counter-selectable marker, allowing for selection of cloning eve

167 LIUM), a vector that contains vermilion as a selectable marker, an attB sequence to allow for phiC31-

168 his fragment encodes the tag, a loxP site, a selectable marker, an exogenous nmt1 promoter and a seco

169 has been implemented as a selectable/counter-selectable marker, but counter-selection escape frequenc

170 ncoding constructs are co-transformed with a selectable marker, high frequencies of genome modificati

171 A drug-selectable marker, human dihydrofolate reductase, added

172 finding was the transforming activity of the selectable marker, hyg.

173 ured for a specific application, to remove a selectable marker, it could be the first of more to come

174 th a fourth cassette for the expression of a selectable marker, resulted in down-regulation of all th

175 ogous FHV RNA2 that expressed the yeast HIS3 selectable marker, resulting in formation of HIS+ coloni

176 We describe the use of a novel selectable marker, the P. pastoris formaldehyde dehydrog

177 with two targeting hooks, HIS3 as a positive selectable marker, URA3 as a negative selectable marker

178 od of delivery, we also demonstrate DNA- and selectable marker-free gene mutagenesis in maize and rec

179 Here, we report high-frequency, selectable marker-free intra-genomic gene targeting (GT)

180 ource efficiency of producing true-breeding, selectable marker-free transgenic barley.

181 ple along with Agrobacterium strain LBA4404, selectable marker-free, gus homozygous lines were eventu

182 quickly and easily without leaving behind a selectable marker.

183 s inhibitor that is often used as a dominant selectable marker.

184 ar DNA fragments containing a telomere and a selectable marker.

185 one marrow cells was induced using DHFR as a selectable marker.

186 ating single-locus scm mutations linked to a selectable marker.

187 rgetrons with a retrotransposition-activated selectable marker.

188 gene cloned in a BAC without the use of any selectable marker.

189 providing a stage-specific positive/negative selectable marker.

190 he human IL-2Ralpha chain (huCD25) gene as a selectable marker.

191 n-phleomycin resistance (GFP-PHLEO) reporter/selectable marker.

192 the nerve growth factor receptor (NGFR) as a selectable marker.

193 to the transformation vector as an auxiliary selectable marker.

194 es revealed 50% of clones expressing the neo selectable marker.

195 of published compliant counterselectable and selectable markers (sacB, pheS, gat and the arabinose-ut

196 By design, selectable markers act as genomic anchor points, and the

197 entities composed of origins of replication, selectable markers and other components.

198 oaches can be limited by the availability of selectable markers and the time required to generate tra

199 Negative selectable markers are useful tools for forward-genetic

200 Selectable markers are widely used in transgenesis and g

201 ed unsuccessful; despite integration of drug selectable markers at the target locus, the gene for PTR

202 These selectable markers confer antibiotic resistance in a wid

203 icon and showing that at least two different selectable markers could be simultaneously expressed by

204 Selectable markers derived from plant genes circumvent t

205 ance) and negative (I-SceI recognition site) selectable markers for isolation of mutants and subseque

206 ideal as environmentally-friendly, dominant, selectable markers for plant transformation.

207 S can serve as important additional dominant selectable markers for the genetic transformation of C.

208 acement of a conjugal origin of transfer and selectable markers in donor and recipient genomes enable

209 rase, has become one of the most widely used selectable markers in plant transformation.

210 enerating transgenic parasites carrying drug selectable markers in subtelomeric and central var loci

211 DNA constructs, transcript read-through from selectable markers is also possible, which again has the

212 combination, nkuA delta and the heterologous selectable markers make up a very efficient gene-targeti

213 For seamless BAC mutagenesis, selectable markers need to be removed after isolation of

214 Selectable markers of bacterial origin such as the neomy

215 unmutated cells and circumvents the need for selectable markers or counter-selection systems.

216 Here we present split selectable markers that each allow for selection of mult

217 trovirus carrying both positive and negative selectable markers that integrated randomly into individ

218 neous keratinocyte stem cells: a pair of new selectable markers that recognize a subset of alpha6(+)C

219 three vector series that rely upon different selectable markers to direct the expression of tagged/un

220 easily modified simultaneously by leveraging selectable markers within 500 kb of the target sites.

221 t transformation vectors containing suitable selectable markers, ability to regulate transgene expres

222 four different internal ribosome entry site-selectable markers, allowing high-efficiency selection o

223 idly isolate rare recombinants without using selectable markers, allowing one-step seamless BAC mutag

224 tolC counter-selection as effective as most selectable markers, and adding a valuable tool to the ge

225 to be coupled to modular, species-compatible selectable markers, promoters and transgenesis facilitat

226 he viability of these mutant omr1 alleles as selectable markers, respectively.

227 tates selection of transgenic plants without selectable markers.

228 s is close to 100%, eliminating the need for selectable markers.

229 assembly process to activate vector-encoded selectable markers.

230 ved genes as dual-purpose selectable/counter-selectable markers.

231 based on in vitro analysis or expression of selectable markers.

232 ficial chromosome (BAC) recombinants require selectable markers.

233 are limited by available surface markers or selectable metabolic characteristics, the MB-based metho

234 tipass separations around the cIM, providing selectable mobility resolution, the instrument design an

235 s, (135)Ba and (137)Ba, mixed at a given and selectable molar ratio.

236 ins that use flavins as cofactors to destroy selectable molecular targets under hypoxic or even anoxi

237 d, and film, and can be well controlled by a selectable motion direction of receiver substrates at a

238 ns for which there are yeast homologues with selectable mutant phenotypes.

239 tope-tagged human IGF-1 fusion protein and a selectable neomycin resistance gene.

240 sefulness of a large bioresource of genotype-selectable normal donors from whom fresh, primary cells

241 fusion, are induced by red light to express selectable or "scorable" marker genes containing promote

242 ore rapid adaptive responses are possible if selectable or plastic phenotypic variation is produced b

243 n as targets for this study because of their selectable or screenable phenotypes (adh1, allyl alcohol

244 d, which corresponds to a MWP filter with 12 selectable passbands.

245 ties were isolated using biologically active selectable peptide technology, which involves functional

246 Twelve NF-kappaB-activating selectable peptides (NASPs) representing specific fragme

247 d protease function by linking activity to a selectable phenotype in E. coli.

248 in the genome onto almost any screenable or selectable phenotype in less than a week.

249 n of a sacB-containing cassette and thus the selectable phenotype of sucrose resistance (Suc(R)).

250 vation or inactivation of genes leading to a selectable phenotype, and (ii) asymmetrical regions of h

251 ate DNA until DNA uptake and expression of a selectable phenotype, as well as the relationship of tra

252 rge number of sequence variants with an easy selectable phenotype.

253 nsertion and excision result in two distinct selectable phenotypes, we have demonstrated that IS5 can

254 phage-associated genes may provide specific, selectable phenotypic traits during co-evolution of myco

255 rial genome for mutations that contribute to selectable phenotypic variation between an evolved strai

256 epigenetic role of developmental biology in selectable phenotypic variation, as well as the largely

257 regulatory changes needed to generate viable selectable phenotypic variation, increase the variety of

258 e used N. sylvestris plants with transgenic, selectable plastids and wild-type mitochondria.

259 ss of the predicted peptides, including user-selectable post-translational modifications.

260 lts indicate that the CD4 versus CD8 fate of selectable precursors can be influenced by the selection

261 uccessful attempt to achieve a stable, under selectable pressure, chloroplast transformation in Cyani

262 oes not rely on any particular screenable or selectable property of the substrates or product, it rep

263 an expression library were challenged with a selectable pseudotype virus packaged by MBG glycoprotein

264 ighlighted directly on the sequence; and the selectable raw sequence.

265 r cell line that provides a quantifiable and selectable readout of intracellular EPO levels and perfo

266 l liver cultures or after electroporation of selectable replicons.

267 rochromatin, we built a strain harboring two selectable reporter genes that are silenced by DNA methy

268 Selectable revertants of TX545 can be generated through

269 ntly triggered co-silencing of a gene with a selectable RNAi-induced phenotype (encoding tryptophan s

270 and TNT could together be used to provide a selectable screen for plastid transformation.

271 ll renderings or testing the effects of user-selectable settings, and lattice imaging poses unique ch

272 otypic (replicable sequence) and phenotypic (selectable shape) properties.

273 50 bp, and as few as 5 bp on one flank of a selectable single nucleotide mutation is sufficient subs

274 the tobacco plastid genome downstream of the selectable spectinomcyin resistance (aadA) marker gene.

275 Arabidopsis leaves with a vector carrying a selectable spectinomycin resistance (aadA) gene and gfp,

276 Using a selectable subgenomic HCV replicon cell culture system,

277 Dicistronic, selectable subgenomic replicons derived from the Con1 st

278 Here, we established efficient RHV-rn1 selectable subgenomic replicons with and without reporte

279 non-genetic diversity in motile behavior is selectable, supporting earlier hypotheses that such dive

280 The selectable system that we have developed provides a vers

281 adout in Eschericha coli and a plasmid-based selectable system to evaluate the influence of strand bi

282 Using this selectable system, we determined the frequency of sponta

283 e Aprt locus on chromosome 8 was used as the selectable target for the study of spontaneous and ioniz

284 inhibit multiple gammac-cytokines but with a selectable target spectrum.

285 We hypothesize that these glycosylations are selectable targets for treating the dermal tropism assoc

286 t of the coding sequence was replaced with a selectable thiostrepton resistance marker.

287 roach in device architecture to realize bias-selectable three-color shortwave-midwave-longwave infrar

288 assessment (CVA-simplified version of Dundee Selectable Threshold Method - DSTM), bitewing radiograph

289 ne conversions that restored function at the selectable thymidine kinase locus.

290 r results demonstrate that evolvability is a selectable trait and allow for the explanation of a larg

291 diversity in a clonal population could be a selectable trait that enables adaptation to environmenta

292 sizes the importance of denitrification as a selectable trait that may influence speciation and adapt

293 Our work implies that noise is a selectable trait tunable by evolution.

294 However, when mutation rate was a selectable trait, the population initially maintained hi

295 translational accuracy is an evolutionarily selectable trait.

296 mRNA library only those mRNAs that encode a selectable unnatural amino acid upstream of a peptide sp

297 omoter and had the coat gene replaced by the selectable URA3 gene.

298 e potential role of development in modifying selectable variation in the evolution of Drosophila sex

299 We used selectable vectors expressing short hairpin RNA (shRNA)

300 length HPV16 genome, when linked in cis to a selectable yeast marker gene, either TRP1 or URA3, could