ライフサイエンスコーパス: sequencing

1 -throughput nature of 16S rRNA gene amplicon sequencing.

2 s, six by exome sequencing and one by genome sequencing.

3 and their striatal integration with movement sequencing.

4 hich are physically phased using linked-read sequencing.

5 s in KID-KCs, as detected by genome-wide RNA sequencing.

6 the depth and resolution of single-cell RNA sequencing.

7 from clinical samples using R9.4.1 Nanopore sequencing.

8 ected and analyzed by 16S ribosomal RNA gene sequencing.

9 ignificantly reduce the cost of reagents and sequencing.

10 ted by laser-capture microdissection for RNA sequencing.

11 esent the first application of AI-ETD to mAb sequencing.

12 ion by both mRNA-based and genomic DNA-based sequencing.

13 tions and, in particular, to improve protein sequencing.

14 ch condition was then assessed by transposon-sequencing.

15 mber that can be measured by high-throughput sequencing.

16 ction and genotypes determined by subgenomic sequencing.

17 in conformation capture with high-throughput sequencing.

18 -derived macrophages and microglia using RNA sequencing.

19 ated genes detectable only through long-read sequencing.

20 ally important traits and from genotyping-by-sequencing.

21 affolding capabilities of single-cell strand sequencing(1,2) with continuous long-read or high-fideli

22 re resistant by either phenotypic testing or sequencing, 78.6% (95% CI 76.1%-80.9%) had resistance-co

23 n-NAFLD controls are analyzed using 16S rRNA sequencing; an independent Western cohort is used for ex

24 Mechanistically, single-cell RNA-sequencing analyses of a mesenchymal niche model showed

25 RNA- and ATAC-sequencing analyses of tumor-derived cell lines revealed

26 This flexible tool is now part of routine sequencing analysis at the Department of Medical Genetic

27 Further, RNA sequencing analysis revealed altered gene expression in

28 , we performed chromatin immunoprecipitation sequencing analysis using patient-derived CIC-DUX4 cells

29 these two qPCR products was confirmed by DNA sequencing analysis, which showed 99-100% similarity wit

30 Small subunit 18S rRNA gene sequencing and accessory pigment analysis displayed simi

31 s are lacking.METHODSWe used next-generation sequencing and array-based DNA methylation profiling to

32 r male offspring, exclusively) by separately sequencing and assembling each type of female and the ma

33 Next-generation sequencing and bioinformatics analysis in the present st

34 healthy volunteers processed with identical sequencing and bioinformatics pipelines.

35 We applied single-cell RNA sequencing and computational modelling to track memory d

36 R repertoire was analyzed by next-generation sequencing and cytokine levels were determined in SAT.

37 Single-cell RNA sequencing and direct comparison to fetal specimens sugg

38 Using RNA sequencing and drug screening, we find that treatment of

39 with chromatin immunoprecipitation with deep sequencing and functional characterization conducted in

40 Finally, we highlight the utility of RNA sequencing and genetic tools in uncovering RGC type-spec

41 ical modifications with direct long-read RNA sequencing and machine learning to detect secondary stru

42 c causes of monogenic diabetes, six by exome sequencing and one by genome sequencing.

43 c cells during seeding using single-cell RNA sequencing and patient-derived-xenograft models of breas

44 similar conclusions from 16S versus shotgun sequencing and reveals both known and candidate pathways

45 G12D and G12V mice were identified using RNA sequencing and reverse-phase protein array analyses.

46 Chromatin immunoprecipitation sequencing and RNA sequencing studies of S180A knock-in

47 RNA-sequencing and stem cell pathway real-time RT-PCR analys

48 Using single-cell and bulk TCR sequencing and structural affinity analyses of cytomegal

49 ccess to the data and also allows for future sequencing and synthesis technologies, by changing the p

50 By studying whole-exome sequencing and whole-genome bisulfite sequencing of cell

51 ession by RNA-sequencing, whole-genome/exome sequencing, and clinical covariates in 134 neuroblastoma

52 hromatin immunoprecipitation sequencing, RNA sequencing, and expression quantitative trait loci data.

53 Thus, using a cohort-based whole-genome-sequencing approach in the diagnosis of PID can increase

54 We review sequencing approaches that have been used for various pa

55 Next-generation sequencing approaches to map RNA-DNA hybrids, a componen

56 However, new sequencing approaches, particularly whole-genome sequenc

57 Gut microbiota data obtained by DNA sequencing are complex and compositional because of larg

58 tance determination, such as by whole-genome sequencing, are required.

59 , the increasing application of whole-genome sequencing as a diagnostic tool is leading to the profil

60 a high-throughput phage immunoprecipitation sequencing assay covering the complete reference protein

61 The increasing implementation of clinical sequencing at diagnosis provides an opportunity to ident

62 ng tumor DNA (ctDNA) fractions together with sequencing background noise and potential tumor heteroge

63 and integrated chromatin immunoprecipitation-sequencing-based analyses demonstrate that direct readou

64 Whole-genome deep sequencing-based analysis has revealed unexpectedly rich

65 s were identified for three lines using deep sequencing-based bulked segregant analysis, and in one c

66 T3 signaling in vitro, while next generation sequencing broadly implicates TrkB.T1 in the PI3K signal

67 Whole-transcriptome sequencing by RNA sequencing (RNA-Seq), with appropriate

68 Although bulk sequencing can provide information about leukaemia biolo

69 he way to cost-effective single-molecule DNA sequencing, capable of handling widely varying GC-bias,

70 analysis of nuclease genome-wide effects by sequencing' (CHANGE-seq), a scalable, automatable tagmen

71 s for high-throughput efficiency and the new sequencing chemistry removes limitations bound by sequen

72 y, chromatin immunoprecipitation followed by sequencing (ChIP-seq) remains a tedious (>2 d), manually

73 unoprecipitation followed by next-generation sequencing (ChIP-seq) was used to assess a range of N-te

74 for the first time in-depth single-cell RNA sequencing, combined with spatial transcriptomics and im

75 Retrospective whole-exome sequencing confirmed a homozygous splice-site mutation i

76 SNP)-trait associations, which leads to high sequencing cost.

77 Using single-cell RNA sequencing coupled with high-resolution in situ hybridiz

78 SA-Seq data analysis require relatively high sequencing coverage to detect significant single nucleot

79 therapy using live imaging, single cell RNA sequencing, CRISPR interference, and pharmacology.

80 douridylation of the miRNAs, high-throughput sequencing crosslinking immunoprecipitation (HITS-CLIP)

81 phylogenomic analysis based on genotyping-by-sequencing data [6] of the 15 species of Scalesia (Darwi

82 RNA-sequencing data analysis shows that Lbs are expressed as

83 e of CNVs from SNP array and next-generation sequencing data are available.

84 Sequencing data are often summarized at different annota

85 ng clustering techniques applied to targeted sequencing data derived from a large unselected populati

86 Whole genome sequencing data did not identify a strain genotype-disea

87 Atlas (TCGA), which aggregated whole-genome sequencing data from 2,658 cancers across 38 tumor types

88 Sequencing data from a single biopsy represent a snapsho

89 al entry-associated genes in single-cell RNA-sequencing data from multiple tissues from healthy human

90 EC specificity, we analyzed single-cell RNA sequencing data from tissue-specific mouse ECs generated

91 Bioinformatics analysis of RNA sequencing data identifies non-productive splicing event

92 gnostic workflow for analysis of metagenomic sequencing data obtained from clinical samples using R9.

93 d for each species separately and metagenome sequencing data revealing adaptive mutations during the

94 medically important mites based on total RNA sequencing data sets generated in this study as well as

95 The comparison with neonatal single-nucleus sequencing data showed a different cellular composition

96 nalyzed and categorized with single-cell RNA sequencing data to perform cluster identification.

97 e quantification of strains from metagenomic sequencing data, enabling the identification of genes th

98 Using matched whole-genome sequencing data, we associated several categories of RNA

99 and statistical analyses of T cell receptor sequencing data, we develop a quantitative theory of hum

100 ans from collecting tissues to obtaining raw sequencing data, with additional time required for data

101 f genes and genomic regions from whole exome sequencing data.

102 eline that processes raw ribosomal profiling sequencing data.

103 ith continuous long-read or high-fidelity(3) sequencing data.

104 - in particular the analysis of metagenomic sequencing data.

105 on from different tissues in single-cell RNA-sequencing data.

106 sase-accessible chromatin in single cells by sequencing defined the transcription factor NFE2L2/NRF2

107 RNA-sequencing demonstrated Acod1 (Aconitate decarboxylase 1

108 is thus of interest to eliminate the use of sequencing depth and analyze the original count data dir

109 sequenced in the PE150 format with a minimum sequencing depth of 5 million merge-able reads to enable

110 ill study these communities by isolating and sequencing DNA directly from an environment (metagenomic

111 assay (MPRA), short barcodes are counted by sequencing DNA libraries transfected into cells and the

112 Here, we performed Direct RNA Sequencing (DRS) using the latest Oxford Nanopore Techno

113 -linking and immunoprecipitation followed by sequencing (eCLIP-seq) in primary hepatocytes induced wi

114 and LEO1) based on this large-scale targeted sequencing effort.

115 ts (average 5/patient) identified from tumor sequencing, enabling ctDNA detection in 96% of patients

116 Sequencing errors and convergent evolution did not accou

117 ror (RMSPE) than when using no tool (leaving sequencing errors uncorrected).

118 tations to target in a follow-up single-cell sequencing experiment, thereby decreasing cost.

119 High-throughput sequencing experiments followed by differential expressi

120 ing using RNA-seq has increased the scope of sequencing experiments to include more complex designs,

121 urther improvements for such native top-down sequencing experiments were possible by leveraging IM se

122 information, such as that from microarray or sequencing experiments.

123 Whole genome and exome sequencing followed by Sanger confirmation were performe

124 ing of 87 tumors with matching transcriptome sequencing for 63 tumors was performed.

125 Here we demonstrate the power of metagenomic sequencing for identifying ongoing outbreaks and their e

126 technical challenges that have prevented HIV sequencing from being used routinely in public health ef

127 Here, we integrate SVs with RNA-sequencing from human post-mortem brains to quantify the

128 RNA-sequencing from post-mortem AD human brains shows downre

129 Genotyping-by-Sequencing (GBS) was applied in a set of 53 diploid Prun

130 Illumina sequencing has allowed for population-level surveys of t

131 Single cell sequencing has become a powerful tool to study the genom

132 Transposon sequencing has been employed to define the core essentia

133 As access to whole-genome sequencing has grown, greater amounts of molecular data

134 However, errors in sequencing have made any estimate of within-sample (alph

135 e present Hybridization of Probes to RNA for sequencing (HyPR-seq), a method to sensitively quantify

136 Whole-exome sequencing identified hemi- and heterozygous variants in

137 Finally, deep sequencing illustrates DrBHV super-infections in individ

138 Surprisingly, single-cell RNA-Sequencing implicated glia, not neurons, in this effect;

139 Although long-read sequencing improves this issue, it is not amenable to pr

140 ing, we compare trial enrollment using ctDNA sequencing in 1,687 patients with advanced gastrointesti

141 We performed whole-exome sequencing in 222 OCD parent-child trios (184 trios afte

142 o address this gap, we performed whole-exome sequencing in 58 men with unexplained meiotic arrest and

143 eys of these domains from the second Newborn Sequencing in Genomic Medicine and Public Health (NSIGHT

144 Whole genome sequencing in multiplex families, proband-parent trios,

145 mple multiplexing method for single-cell RNA sequencing in which fixed cells are chemically labeled b

146 High-throughput sequencing, liposomal nanoallergen display, bead-based a

147 ied ITT was 70% according to next-generation sequencing (<10-5 sensitivity).

148 Integrated RNA sequencing, metabolomics, and molecular analyses showed

149 We addressed this using RNA sequencing metagenomics(4-6) of placental samples from n

150 We developed a straightforward U-DNA sequencing method (U-DNA-Seq) that was combined with in

151 The sequencing method achieved very high coverage (median 99

152 scopically and by a specific PCR followed by sequencing method in two of three cardiovascular tissues

153 An unbiased protein sequencing method, with a dynamic range that covers the

154 op a technique, named MNase hypersensitivity sequencing (MH-seq), to identify genomic regions associa

155 ng miRNA processing machinery at synapse and sequencing miRNAs and analyzing their functions in synap

156 he samples were analyzed using 16S rRNA-gene sequencing (MiSeq-Illumina) and QIIME pipeline.

157 Motion Sequencing (MoSeq) is an ethologically inspired behavior

158 ination of multiplex SNaPshot assays, Sanger sequencing, mutation-specific PCR, or droplet digital PC

159 sults: White PI Z heterozygotes confirmed by sequencing (MZ; n = 74) had lower post-bronchodilator FE

160 cell receptors (TCRs) using next-generation sequencing (NGS) in neonates, infants, and children can

161 s combined with the rapidly falling costs of sequencing now allows comparisons between multiple exper

162 Sequencing of 1,314 SARS-CoV-2 viral genomes from availa

163 Whole-exome sequencing of 159 prospectively resected pituitary adeno

164 etic variants were identified by whole-exome sequencing of 2 patients with familial IMD.

165 opathic NOA and SO, we performed whole-exome sequencing of 314 unrelated patients of Chinese Han orig

166 enome, exome, transcriptome, and methylation sequencing of 83 canine gliomas, we found alterations sh

167 g on palms, soles or nail beds, whole genome sequencing of 87 tumors with matching transcriptome sequ

168 ted in vivo by chromatin immunoprecipitation sequencing of a patient-derived xenograft.

169 replication, we performed follow-up targeted sequencing of ASH1L in additional 524 unrelated TS sampl

170 eoclast ablation by denosumab (DMAb) and RNA-sequencing of bone biopsies from postmenopausal women to

171 -exome sequencing and whole-genome bisulfite sequencing of cell free DNA (cfDNA) and of matched metas

172 performed a gene expression study using RNA sequencing of CNON cells from 111 control subjects and 1

173 Metagenomics is the collective DNA sequencing of coexisting microbial organisms in an envir

174 expression associated with CUD by using RNA sequencing of dorsal-lateral prefrontal cortex neurons.

175 Single-cell RNA sequencing of five TNBCs revealed two cancer-associated

176 Sequencing of gut transcripts revealed PE-fed larvae ret

177 he profiling of bacterial communities by the sequencing of housekeeping genes such as that encoding t

178 controlled trial of rapid diagnostic genomic sequencing of infants in regional ICUs.

179 ding, biocytin staining, and single-cell RNA sequencing of more than 1,300 neurons in adult mouse pri

180 Here, using next-generation sequencing of near-full-length single HIV-1 genomes and

181 cing (RenSeq) with single-molecule real-time sequencing of PacBio for 18 accessions in Solanaceae, in

182 Single-cell RNA-sequencing of plaque immune cells revealed that unlike T

183 results were analyzed by bi-directional PCR/sequencing of residual blood culture samples.

184 Sequencing of RNA from the endothelial cells uncovered t

185 diversity was examined using high-throughput sequencing of rpoB amplicons generated with a Streptomyc

186 Recently, sequencing of salivary-gland mRNA libraries revealed inc

187 SWGA enables the successful whole genome sequencing of samples with low parasite density (i.e. on

188 Whole genome sequencing of select transformants demonstrated that rec

189 16S rRNA gene sequencing of SIgA-coated/uncoated bacteria (IgA-Biome)

190 However, genome sequencing of specimens is challenging because of low ba

191 isease.Methods: We performed single-cell RNA sequencing of sputum cells from nine subjects with CF an

192 otor methylation was quantified using Sanger sequencing of the CpG sites 74-98 within the MGMT promot

193 Single-cell RNA sequencing of the immune compartment showed that IL8 is

194 =10(4) CFU/ml of N. gonorrhoeae was present, sequencing of the large majority of the genome was frequ

195 omes of sheep, with a focus on isolation and sequencing of the ovine Y chromosome.

196 MIC, pDST and whole genome sequencing of the pncA, rpsA and panD genes were used to

197 After sequencing of the remaining intact strands, this allows

198 Shotgun sequencing of vaginal swabs from postmenopausal women se

199 Single-cell RNA sequencing of vascular cells in mice suggested that the

200 To address these questions, we performed 16S sequencing on fecal DNA samples from thirty-nine bighorn

201 n problem, and have generated high depth RNA-sequencing on FUS mutants in parallel to FUS knockout, a

202 We performed whole exome sequencing on this cohort and applied 2 hypotheses to ou

203 ent, we performed genome-wide RNA tomography sequencing on zebrafish, chicken, mouse, and human embry

204 We used the TruSight Cardio sequencing panel to evaluate the burden of rare variants

205 troduce probabilistic cell typing by in situ sequencing (pciSeq), an approach that leverages previous

206 ression profiling by tagging RNA in situ and sequencing (PETRI-seq)-a low-cost, high-throughput proka

207 ven the increased accessibility of microbial sequencing platforms, many recent studies have investiga

208 resent RNA structure analysis using nanopore sequencing (PORE-cupine), which combines structure probi

209 The sequencing process generates a huge amount of data in th

210 In two independent ongoing next-generation sequencing projects for individuals with holoprosencepha

211 ese QC measures reflect known differences in sequencing protocol and provide a guide to downstream an

212 software tool that utilizes next-generation sequencing read depth information and variant allele fre

213 nchmarking workflow, using synthetic shotgun sequencing reads for which we know the true CDS content

214 g DuploMap-aligned PacBio circular consensus sequencing reads, an additional 8.9 Mb of DNA sequence w

215 ces, we conducted resistance gene enrichment sequencing (RenSeq) with single-molecule real-time seque

216 nical management after the ultra-rapid exome sequencing report, the time from hospital admission to t

217 profiling coupled with T cell receptor (TCR) sequencing reveal lineage connections in T cell populati

218 Whole genome sequencing revealed differences in putative virulence ge

219 Microarray analysis and single-cell RNA sequencing revealed that a number of cytokine-inducible

220 Single-cell mRNA sequencing revealed that systemic Akt1 deletion maintain

221 Genomic sequencing revolutionized the epidemiological understand

222 Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent s

223 tically regulated expression solely from RNA-sequencing (RNA-seq) datasets.

224 e compiled a metadataset composed of 876 RNA-sequencing (RNA-Seq) samples from five publicly availabl

225 Whole-transcriptome sequencing by RNA sequencing (RNA-Seq), with appropriate bioinformatics, p

226 toid arthritis to allow for longitudinal RNA sequencing (RNA-seq).

227 examined with chromatin immunoprecipitation sequencing, RNA sequencing, and expression quantitative

228 example, batch annotation of TCR repertoire sequencing samples allows for annotating large datasets

229 trieved references for 257 real whole-genome sequencing samples with a mean of >99.8% identity to ass

230 Single-cell RNA-sequencing (scRNA-seq) allows us to dissect transcriptio

231 Using single-cell RNA-sequencing (scRNA-seq) and genetic reporter mice, we ide

232 ntegrated recently published single-cell RNA-sequencing (scRNA-seq) data from 727 peripheral and nerv

233 nalyzed previously generated single-cell RNA-sequencing (scRNA-seq) data of gastric corpus epithelium

234 The development of single-cell RNA sequencing (scRNA-seq) has allowed high-resolution analy

235 Single-cell RNA sequencing (scRNA-seq) has enabled the simultaneous tran

236 f blinding diseases, we used single-cell RNA-sequencing (scRNA-seq) to analyze the transcriptomes of

237 Here, we employed single-cell RNA sequencing (scRNA-seq) to examine the immature postnatal

238 Using single-cell RNA sequencing (scRNA-seq), we have identified a population

239 Single-cell sequencing (SCS) has impacted many areas of cancer resea

240 rected by the high-quality second-generation sequencing (SGS) short reads, which is referred to as hy

241 Single-cell RNA sequencing showed that SNP-IV induced stem-like genes (T

242 Viral genome sequencing showed that the majority of HCWs had the domi

243 p a "Shotgun" Ion Mobility Mass Spectrometry Sequencing (SIMMS(2)) method in which intact HS sacchari

244 Recent advances in long-read sequencing solve inaccuracies in alternative transcript

245 simultaneously perform bulk and single-cell sequencing, some studies have analyzed initial bulk data

246 design of tandem mass spectrometry-based CAD sequencing strategies for mixtures of lignin degradation

247 Recent sequencing studies at the bulk and single-cell level in

248 matin immunoprecipitation sequencing and RNA sequencing studies of S180A knock-in cells demonstrated

249 th the reduction in price of next-generation sequencing technologies, gene expression profiling using

250 Using long-read sequencing technologies, we provide a high-quality genom

251 nventional genetic tools and next-generation sequencing technologies.

252 introduced Pacific Biosciences (PacBio) HiFi sequencing technology bridges this divide by delivering

253 n assembly using reads from either a certain sequencing technology or a small assembly.

254 The error-prone third-generation sequencing (TGS) long reads can be corrected by the high

255 By sequencing the whole genome of MERS-CoV from persistentl

256 In this study, we used transposon sequencing (TN-seq) to understand how the responses to o

257 We applied nanopore-based direct RNA sequencing to characterize the developmental polyadenyla

258 sess Treg stability such as bisulfite Sanger sequencing to determine the methylation status of the Tr

259 bial samples were analyzed with 16S amplicon sequencing to identify bacterial profiles in young (3 to

260 ed crosslinking immunoprecipitation- and RNA-sequencing to identify the AGO1-mediated mechanisms unde

261 Here, we employed single-cell RNA-sequencing to interrogate aging-related changes in the H

262 reasoned that breadth may supplant depth of sequencing to overcome the barrier of cfDNA abundance.

263 We used single-cell RNA sequencing to profile human skeletal muscle tissues from

264 4-second LC-MS method, and barcoded nanopore sequencing to rapidly isolate and characterise the best

265 We used next-generation sequencing to study 214 patients with SMM.

266 ion on NF admission, we applied whole-genome sequencing to track the spread of 4 ARO species across r

267 raries, combined with T cell cloning and TCR sequencing, to dissect the human naive and memory CD4+ T

268 Single-cell RNA sequencing uncovered three epicardial subpopulations wit

269 tients and 4,982 controls by next generation sequencing using molecular inversion probes that targete

270 a, particularly when annotating whole-genome sequencing variants against a huge database with billion

271 ing volume of functional annotation data and sequencing variants, existing variant annotation algorit

272 Whole genome sequencing was performed on 20 matched blood and aqueous

273 Exome sequencing was performed on 62 HGT1 and 15 matched norma

274 Whole exome sequencing was performed on all patients.

275 16S ribosomal RNA gene sequencing was performed on sputum from 253 clinically s

276 cibility of meropenem resistance, short read sequencing was performed using an Illumina NextSeq500 se

277 Whole genome sequencing was used to uncover a frame-shift insertion a

278 l community response measured using amplicon sequencing, we combine initial trait data measured for e

279 Using single-cell RNA sequencing, we found that in GA lesions IFN-gamma produc

280 Using allele-specific single-cell RNA sequencing, we here estimate the two noise components of

281 egion and, using third generation, long-read sequencing, we identified a 120 kb insertion in the wing

282 Using whole genome sequencing, we identified a heterozygous frameshift vari

283 ute-immunoprecipitations and high-throughput sequencing, we provide experimental evidence for the dev

284 Using nascent RNA sequencing, we show that an AS15 analogue triggers the u

285 Using single-cell RNA sequencing, we show that these cells are largely activat

286 internal transcribed spacer (ITS) and D1/D2 sequencing were successfully employed for isolate clade

287 polymerase chain reaction and amplicon deep sequencing were used to track species and clone-specific

288 he use of targeted panels versus whole-exome sequencing (WES).

289 ocus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) analysis.

290 s within 7 days were chosen for whole-genome sequencing (WGS) and a phylogenetic analysis was perform

291 landscape of myeloma using deep whole-genome sequencing (WGS) and develop a model that identifies pat

292 Whole-genome sequencing (WGS) can support surveillance by pinpointing

293 Here we perform whole-genome sequencing (WGS) of 103 UM from all sites of the uveal t

294 Established methods for whole-genome-sequencing (WGS) technology allow for the detection of s

295 encing approaches, particularly whole-genome sequencing (WGS), have dramatically changed the landscap

296 ions of B cells via bulk and single-cell RNA sequencing, which demonstrate clonal expansion and uniqu

297 elomeres (ALT)], TERT mRNA expression by RNA-sequencing, whole-genome/exome sequencing, and clinical

298 lenge, combining long-range PCR and nanopore sequencing with a novel bioinformatics pipeline.

299 We find that nanopore sequencing with Hi-C scaffolding produces highly contigu

300 c landscapes of N/S HNSTs.RESULTSWhole-exome sequencing within a precision oncology program identifie