ライフサイエンスコーパス: serial passage

1 using an experimental evolutionary approach (serial passage).

2 r than that of wild type, predominated after serial passage.

3 ls, which may lead to a loss of fitness upon serial passage.

4 , DcR1, TMS1, CRBP1, HIC-1, and RRAD) during serial passage.

5 and infection is lost from the culture upon serial passage.

6 tability, and the EMCV IRES was deleted upon serial passage.

7 ained relatively constant over the course of serial passage.

8 to the numerical bottlenecks associated with serial passage.

9 nt coding region of HA vRNA were found after serial passage.

10 iffer in the pattern of packaged DVGs during serial passage.

11 se, as p19(Arf)-/- MEFs do not senesce after serial passage.

12 transfected human Huh7.5 hepatoma cells upon serial passage.

13 U/ml) and remained genetically stable during serial passage.

14 ysin and staphyloxanthin inactivation during serial passage.

15 ays were unstable and did not persist during serial passages.

16 of clones differing in plasmid content after serial passages.

17 r these viruses remained constant throughout serial passages.

18 served stability of the chimeric virus after serial passages.

19 for over 4 months in culture encompassing 12 serial passages.

20 nd genetic stability were characterized over serial passages.

21 at the viruses evolved through the course of serial passages.

22 scalating concentrations of oseltamivir over serial passages.

23 ties continued to evolve for as long as four serial passages.

24 , -181b, and -130b expression increased with serial passages.

25 and was genetically stable over 10 rounds of serial passaging.

26 -9) and showed no detectable resistance upon serial passaging.

27 to a prototypic multivalent sialylated PG by serial passaging.

28 f CDC-9 virions from two different stages of serial passaging.

29 kbone could become better adapted to pigs by serial passaging.

30 Through in vivo serial passage, a virulent mouse-adapted strain was obta

31 We show that after 10 serial passages, A/H7N1 developed the ability to be tran

32 ncy, and stem cell properties: self-renewal, serial passaging ability, cycling quiescence, long doubl

33 icrovirid bacteriophage (ID11), we conducted serial passage adaptations at two bottleneck sizes (10(4

34 Through serial passaging, an erlotinib-resistant subline of WHIM

35 s behind increased pathogenicity of SIV upon serial passage and adaptation of SIVs to new hosts follo

36 sity within the type strain before and after serial passage and between different field isolates; (c)

37 que opportunity for the study of the role of serial passage and cross-species transmission was offere

38 The Q493K spike mutation arose early in serial passage and is predicted to provide affinity-enha

39 By serial passage and plaque isolation, we demonstrate that

40 cant phenotypic and genotypic changes during serial passage and suggest that vigilance should be used

41 self-renewal capacity as measured by in vivo serial passage and were restricted to CD34-positive frac

42 rowth of HCV genotype 1a (clone H77) through serial passages and accompanying changes in IHH in respo

43 cally stable in Huh7 cells for at least nine serial passages and did not accumulate attenuating or ce

44 of resistance in this microorganism up to 30 serial passages and growth of intracellular mycobacteria

45 t growth factor-2 (FGF2) for 5-6 consecutive serial passages and were transplanted into the injury si

46 PE from neural stem cells (NSC) during their serial passaging and differentiation.

47 ber and sequence variation despite extensive serial passaging and display exquisitely precise, cell-a

48 e fibroblasts into iPS cells decreases after serial passaging and the concomitant onset of senescence

49 and in vitro by clonogenic growth, prolonged serial passage, and rapid adherence to extracellular mat

50 nfusing phenotype persisted through repeated serial passage, and the phenotype was consistent in four

51 expression profiles that were retained with serial passaging, and they showed a spectrum of somatic

52 After 25 serial passages (approximately 50 replication cycles), m

53 When subjected to selective pressure by serial passage at increasing temperatures, Min B substan

54 During in vitro stress tests involving serial passage at incrementally increasing temperatures,

55 During serial passage at progressively increasing temperature,

56 In contrast, serial passage attenuated the parental HK/483 virus in v

57 d from animal to animal for a total of three serial passages; but HIV replicated poorly in vivo, was

58 These studies demonstrate that serial passage can be used to rapidly evolve a VSV genom

59 Serial passages continuous up to sixth passages in the c

60 We show that stem-like activity in serial passage culture and in vivo breast morphogenesis

61 The plasma inoculum used for the serial passage did not contain adventitious bacterial or

62 Serial passages did not change the viral phenotype as co

63 of a predominantly Fim+ population required serial passage every 48 to 72 h in unshaken brain heart

64 mutants retained wild type infectivity, and serial passage experiments revealed replacements that we

65 Serial passage experiments revealed that the BDMV DNA-A

66 Serial passaging experiments of SW/VA/19 in swine cells

67 ained by plaque purification following eight serial passages expressed CAT, showing that the foreign

68 1N1 internal-protein genes together with the serial passages favour H9N2 virus adaptation to pigs.

69 Serial passage (>40 times) of the three low-passage isol

70 characterization of the cultured CEnC across serial passages has not been performed.

71 ectly in primary rat embryo fibroblasts upon serial passaging, identified nine features that were in

72 After several weeks of serial passage in C8166 cells, surviving viruses were se

73 attenuated (no diarrhea, mild lesions) after serial passage in cell culture.

74 that the viruses regained fitness following serial passage in cell culture.

75 l strain typing of ovine scrapie isolates by serial passage in conventional mice has shown some diver

76 With increasing frequency during serial passage in culture, primary human keratinocytes e

77 diac markers and proliferated rapidly during serial passage in culture, without apparent senescence.

78 e frequency of tdTom(+) cells increased with serial passage in cultured murine embryo fibroblasts fro

79 We performed in vitro serial passage in D2F2/E2 cells to evolve a virus with i

80 ve attenuated vaccines are generated through serial passage in embryonated hens' eggs, an empirical p

81 rowth at 37 degrees C in tissue culture, and serial passage in ferrets.

82 eveloped as attenuated vaccine candidates by serial passage in fibroblasts, have lost the ability to

83 SHIV-1157i was adapted by serial passage in five monkeys, three of which developed

84 In contrast, serial passage in guinea pigs resulted in the selection

85 Serial passage in hamsters resulted in clinical disease

86 of five mutations that were selected during serial passage in Huh-7.5 cells were studied.

87 o these different host environments, we used serial passage in human and mosquito cell lines and esti

88 Serial passage in human cells of a mutant lacking the ga

89 H3) was isolated and successfully adapted to serial passage in human rectal tumor 18 cell cultures.

90 switch from phase-off to phase-on following serial passage in human serum.

91 ertical transmission to progeny worms during serial passage in lab colonies.

92 irus (MSCV) expressing only neo, followed by serial passage in liquid culture containing stem cell fa

93 gy in characterizing TSE isolates throughout serial passage in livestock, which is applicable to a ra

94 tate cancer xenograft (LAPC-9) propagated by serial passage in male severe combined immunodeficient m

95 at the chimeric virus failed to adapt during serial passage in marmosets.

96 decreased significantly (P < 0.0001) during serial passage in MDCK cells inoculated with seasonal in

97 MEL-adapted (MA) mutant viruses isolated by serial passage in MEL cells acquired the capacity to bin

98 d that 10 nucleotide changes occurred during serial passage in mice.

99 ts and that strain fidelity was preserved on serial passage in mice.

100 and tprK sequence diversity accumulates with serial passage in naive rabbits.

101 data are available regarding the effects of serial passage in natural hosts.

102 using a conventional attenuation strategy of serial passage in nonhost animals and cultured cells.

103 We used serial passage in ribavirin, beginning with a variety of

104 Serial passage in subtherapeutic transferrin concentrati

105 lular adhesion molecule 1 (hICAM-1), through serial passage in the lungs of mice transgenic for the h

106 nt HPF3 virus variant (ZM1) was generated by serial passage in the presence of 4-GU-DANA.

107 d cytopathic effects were observed following serial passage in the presence of a noncytopathic helper

108 elect for resistance to both 3TC and PMPA by serial passage in the presence of increasing concentrati

109 N. meningitidis strain 8047 was subjected to serial passage in the presence of P1.2, a PorA-specific

110 on, virus resistant to BDCRB was selected by serial passage in the presence of the compound.

111 ee distinct methods: transposon mutagenesis, serial passage in the presence of tPMP-1 in vitro, or ca

112 Following serial passage in the presence of VV-P1 at 33 or 30 degr

113 The PV-Luc-EMCV replicon was unstable upon serial passage in the presence of VV-P1, with deletions

114 After serial passage in tissue culture, some viruses partially

115 igh-titer stock of pathogenic SHIV-C109p5 by serial passage in two rhesus macaques (RM) and tested it

116 fungal strains, resistance evasive following serial passage in vitro and highly efficacious in animal

117 vel of genetic and phenotypic stability upon serial passage in vitro at restrictive temperatures comp

118 In neuroblastoma cells transformed by serial passage in vitro, leading to more rapid prolifera

119 With serial passage in vitro, wild-type postnatal cortical as

120 on, self renewal, and differentiation during serial passage in vitro.

121 iscent of strain stabilization observed upon serial passage in vivo.

122 to develop pandemic capabilities, even after serial passages in a mammalian host.

123 at the HIV-2 isolate recovered after several serial passages in baboons will be useful in future stud

124 virions typically generated during in vitro serial passages in cell culture of the virus at a high m

125 d with escalating concentrations of F10 over serial passages in cultured cells to select for escape m

126 genetically and phenotypically stable during serial passages in FDA vaccine-approved Vero cells.

127 After serial passages in ferrets, a dominant H1N2 virus popula

128 ble HAV-mediated expression for at least six serial passages in FRhK-4 cells.

129 Additional serial passages in hamsters resulted in the selection of

130 man metastatic samples were maintained after serial passages in mice and emergence of resistance.

131 rted previously the in vivo selection during serial passages in mice of several evolutionary intermed

132 Adaptation of IDV by serial passages in mice was not sufficient to induce dis

133 c H1N1/2009 virus and subjected to only nine serial passages in pigs, acquired greatly enhanced virul

134 keup as the pandemic H1N1/2009 virus to nine serial passages in pigs.

135 to those of the pandemic virus after limited serial passages in pigs.

136 A) recovered from liver homogenates after 24 serial passages in severe combined immunodeficient (SCID

137 ered viral genome was stable following rapid serial passages in vitro and multiple rounds of replicat

138 duced at high titres and were stable through serial passages in vitro.

139 determined by sequence analysis after seven serial passages in VP30-expressing Vero cells.

140 tavirus vaccines have been developed through serial passaging in cell culture and found to be general

141 al rotavirus vaccines have been developed by serial passaging in cell culture and found to be safe in

142 accine generation instead of labor-consuming serial passaging in cell culture.IMPORTANCELive oral rot

143 attenuation mutation site, which arose after serial passaging in culture and led to a loss in lethali

144 f this study was to attenuate this strain by serial passaging in MARC-145 cells and assess its virule

145 a mouse-adapted MARV, Angola variant through serial passaging in mice.

146 nd neuroinvasiveness were derived by limited serial passaging in mouse brain.

147 Two peptides enriched after serial passaging in mouse lungs mediated the retargeting

148 e MUTs after multiple replication cycles and serial passaging in NHBE cells when initial mixtures con

149 selection of the shuffled viral libraries by serial passaging in pt mPBMC, a species emerged which re

150 iciency virus (SHIV) was generated following serial passaging in rhesus macaques.

151 this genus in nature or were altered during serial passaging in the chronically infected cell line.

152 in the 1960s using a traditional approach of serial passaging in tissue culture of the virulent Trini

153 Serial passaging in triple M2e-mAb-treated immunocompete

154 e 6 amino acid mutations that appeared after serial passaging in Vero cells, mutations of wild-type C

155 ing breast cancers was mostly preserved upon serial passaging in xenografts and in short-term culture

156 asles, and rotavirus, have been developed by serial-passaging in cell culture.

157 Analyses of virus from six serial passages indicated that genomes with this insert,

158 umors with considerable fidelity, even after serial passage, irrespective of the histological grade o

159 Furthermore, ongoing clonal dynamics during serial passaging is a feature of tumours experiencing mo

160 the strain is first adapted to mice through serial passaging, it becomes able to cause disease in th

161 The serial passaging led to formation of defective genomes,

162 Serial passaged LNC resulted in gradual loss of nuclear

163 Following 20 to 50 serial passages, mutations were identified and changes i

164 Previous studies have described in vitro serial passage of a Deltagamma(1)34.5 herpes simplex vir

165 The serial passage of a viral insertion mutant demonstrated

166 with live-attenuated vaccines generated via serial passage of a virulent field isolate through embry

167 uated vaccines (LAVs) that are generated via serial passage of a virulent field isolate through embry

168 attenuated vaccines against are generated by serial passage of a virulent isolate in embryonated eggs

169 ated vaccines are traditionally generated by serial passage of a virulent strain in embryonated chick

170 IBV vaccines are currently developed by serial passage of a virulent strain on embryonated hen's

171 During serial passage of A/Ann Arbor/6/1960 at low temperatures

172 ere, we describe escape mutants generated by serial passage of A/Netherlands/602/2009 (H1N1)pdm09 in

173 u5Ac2en, of influenza viruses was studied by serial passage of A/Turkey/Minnesota/833/80 (H4N2) in Ma

174 pid acquisition of adaptive mutations during serial passage of attenuated vaccinia virus (VACV).

175 It has been postulated that serial passage of BCG over the years may have resulted i

176 Island and a second from a goat infected by serial passage of blood from the first infected goat.

177 f the genome, the UL133/8 locus is lost upon serial passage of clinical strains of HCMV in cultured f

178 Serial passage of Cryptococcus neoformans in mice increa

179 Lb' region of the genome that is lost during serial passage of HCMV in cultured fibroblasts.

180 esistance to ABT-378 in vitro was studied by serial passage of HIV-1 (pNL4-3) in MT-4 cells.

181 Notably, serial passage of HIV-1 in an A3.01 clone that expresses

182 A similar result was observed following serial passage of human astrovirus with a deleted s2m se

183 In summary, serial passage of HuNoV in pigs, with occurrence of mild

184 sistance to A-315675 was studied by in vitro serial passage of influenza A/N9 virus strains grown in

185 o these mutagenic agents did not arise after serial passage of influenza virus populations in subleth

186 vvD54-M002, the virus selected after in vivo serial passage of M002 in D54-MG tumors, improves surviv

187 Serial passage of NTHi increased both PCho content and b

188 ability of PIV5 was also demonstrated during serial passage of one strain (W3) in Vero cells at a hig

189 In conclusion, serial passage of PEDV, both in vitro and in vivo, influ

190 Serial passage of primary mammalian cells or strong mito

191 After serial passage of rhtrs1-amplified viruses, there arose

192 During serial passage of simian SA11 rotaviruses in cell cultur

193 e was unstable and sustained deletions after serial passage of SIV(delta nef) vectors in CEM-X-174 ce

194 ere that upon cross-species transmission and serial passage of SIVsab from its natural host, the saba

195 llow fever (YF) virus has been derived after serial passage of strain Asibi through hamsters.

196 disassembly, we selected variant viruses by serial passage of strain type 3 Dearing (T3D) in murine

197 The serial passage of the CA/09 parental virus and the CA/09

198 analyzing mutations that accumulated during serial passage of the HM-175 strain of hepatitis A virus

199 PF) mutants arise at a high frequency during serial passage of the Lymantria dispar nucleopolyhedrovi

200 Serial passage of the monovalent FluMist 2009 H1N1 pande

201 After serial passage of the mutant virus in tissue culture, we

202 Serial passage of the quasispecies in vitro resulted in

203 Serial passage of the resistant cells in mice resulted i

204 Serial passage of the tumor fragments in SCID mice resul

205 ants of simian virus 40 (SV40), generated by serial passage of the virus at high multiplicities of in

206 Serial passage of the virus in TIME cells is completely

207 Serial passage of these constructs in Sk-N-Mc cells yiel

208 Following serial passage of these viruses, the viruses acquired in

209 od, are often generated by repeated in vitro serial passage of this highly cell-associated virus to a

210 r, we observed the emergence of T544I during serial passage of various Ebola Makona isolates on Vero

211 IBV vaccines are currently generated by serial passage of virulent IBV field isolates through em

212 This was demonstrated by serial passage of virus from cell culture supernatants,

213 ne made by the classical empirical method of serial passage of virus in tissue culture cells.

214 rand transcripts into MHV-infected cells and serial passage of virus samples from RNA-transfected cel

215 Serial passage of viruses in cell culture has been tradi

216 ve spreading replication, amplification, and serial passage of wild-type HIV-1.

217 Serial passage of yellow fever 17D virus (YF5.2iv, deriv

218 Following serial passages of a type A(24) Cruzeiro virus (A(24)Cru

219 Twelve serial passages of an Ad2 vector lacking the protein IX

220 an experimental evolution protocol involving serial passages of an attenuated vaccinia virus, rapid a

221 We then performed serial passages of an environmentally isolated C. neofor

222 Following 30 serial passages of ancestral SARS-CoV-2 in mACE2(H353K)

223 For serial passages of CWD isolates in Syrian golden hamster

224 Serial passages of Deltagamma(1)34.5 mutants in human ce

225 ubclinical infection was studied by making 4 serial passages of hamster scrapie agent (263K) in mice.

226 Eleven serial passages of HCV genotype 1a (clone H77) in IHH we

227 able of tumor initiation and self-renewal by serial passages of hepatospheres with chemotherapeutic a

228 he development of disease, we performed five serial passages of HIV-2(UC2) in baboons by using blood

229 losely related CoV, that was generated after serial passages of SARS-CoV in cell culture, we designed

230 Several serial passages of stacked beta-sheet-seeded solutions l

231 Serial passages of the RNase mutants in vitro can also g

232 Serial passages of the virus in MMH-D3 cells under subop

233 Following 10 serial passages of three independent lineages, the bulk

234 ated maVie16, a mouse-adapted SARS-CoV-2, by serial passaging of a human isolate.

235 r responses and equal replication fitness by serial passaging of co-cultures.

236 Furthermore, serial passaging of DV2 in the presence of dasatinib led

237 olve and re-sequence approach, we found that serial passaging of the cross-kingdom fungal pathogen Fu

238 Serial passaging of the DV2 mutant E-Y299F led to the id

239 iarrhea virus (PEDV) strain, PC177, by blind serial passaging of the intestinal contents of a diarrhe

240 Here we show that serial passaging of USA-WA1/2020 strain in mouse lungs r

241 Orthogonally, by serial passaging of virus on EMC-deficient cells, we ide

242 After serial passage on antibiotic-free medium, the isolate ma

243 The virus was adapted by serial passage on Her2/neu-expressing cancer cells resul

244 Serial passage on polarized cell monolayers selected for

245 Serial passages on coated Matrigel resulted in rapid exp

246 etes strains were exposed to voriconazole by serial passages on voriconazole-containing medium.

247 ype in cultured human fibroblasts, mimicking serial passage or ectopic expression of a dominant negat

248 e and do not revert to virulence, even after serial passage or long-term persistent infection in vivo

249 nsfected mammalian cells without the need of serial passage or plaque purification.

250 ctably affect DVG pattern following reovirus serial passage or viral recombination junction profiles.

251 en haplotype patterns which were stable upon serial passage over 1 year.

252 ippocampal progenitor cells in vitro using a serial passaging protocol that mimics the end-replicatio

253 Serial passages provided no evidence that these deletion

254 Subcloning analysis showed that after serial passaging, recloning, and expansion, these cells

255 membrane permeability and ATP release, with serial passaging resulting in a mutation in mlaC, a phos

256 A time course study of serial passages revealed that the 30-day supernatant had

257 ntly, 101LL mice did not transmit disease on serial passage, ruling out the presence of subclinical i

258 During serial passage, S. aureus existed as a heterogeneous pop

259 ve lost the capacity for stumpy formation by serial passage ("selected monomorphs") and analyzed for

260 The HIV-2(UC2) recovered after four serial passages showed increased kinetics of viral repli

261 ell line cultured under conditions that over serial passages specifically allowed for generation and

262 t "immortalize" the cultures and that, after serial passages, sphere conditions maintain TICs, wherea

263 was no significant decrease in the LD(50) of serial passage strains compare to single passage strain

264 Serial passaging studies demonstrated that a two-siRNA c

265 es of CDC-9 virus particles at two stages of serial passaging supports a proposed mechanism for initi

266 n by the host defense system, we developed a serial-passage technique in mice to select for phage mut

267 ia-specific phage strains, combined with the serial-passage technique, may provide insights for devel

268 infection which was sustained for the three serial passages tested.

269 properties and the intensity is increased by serial passaging that are significant in the sixth passa

270 ive advantage in vitro because even after 55 serial passages the original recombinant FPV was still p

271 During transfection and two serial passages, the various miniantigenomes were essent

272 panzees than in the humans, and during eight serial passages there was no change in the sequence of t

273 NA-B pseudorecombinant was maintained during serial passage through N. benthamiana and Phaseolus vulg

274 SHIV-A underwent rapid serial passage through six RMs.

275 These cells were additionally capable of serial passage through three successive generations of e

276 After 30 serial passages through the lungs of KI mice, a mouse-ad

277 scRNA-seq data (53,641 filtered cells) from serial passaging TNBC patient-derived xenograft (PDX) ex

278 llowed by nonhomologous recombination during serial passaging to generate a single, replication-compe

279 The serial passage tumor cells exhibited nondiploid karyotyp

280 ercame the replication block were derived by serial passage under restrictive conditions in either mo

281 d pathogenicity, respectively, and show that serial passage under selection pressure remains an effec

282 n and stablized in the transfected cells via serial passages under puromycin selection.

283 These variants can be identified by serial passaging virus stocks, allowing those with incre

284 After these serial passages, virus levels in plasma, peripheral bloo

285 ion, since YFP(+) neurosphere formation over serial passage was unaffected.

286 over, a decrease in myogenic capacity during serial passaging was concomitant with a gradual increase

287 However, after serial passage we obtained a virus population that grew

288 At least four serial passages were required to stabilize the strain-sp

289 election of a mutant HSV-1 strain by in vivo serial passage, which prolongs survival in two separate

290 differences were faithfully maintained after serial passaging, which implies that alpha-synuclein pro

291 creatic islet cells can be expanded by three serial passages while maintaining their endocrine proper

292 there are no reports on the consequences of serial passage with strong selection pressure on its fit

293 imary biliary cirrhosis when cocultivated in serial passage with supernatants containing the human be

294 sis of the encapsidated replicons after four serial passages with VV-P1 revealed that the dicistronic

295 for enhanced antitumor efficacy via in vivo serial passage within flank D54-MG tumor xenografts.