ライフサイエンスコーパス: sialylation

1 mortality (q = 0.04 for galactosylation and sialylation).

2 s and significantly elevated levels of rhEPO sialylation.

3 rom GSL biosynthesis and toward glycoprotein sialylation.

4 lation and mannose content, while decreasing sialylation.

5 ll types of cancers and results in increased sialylation.

6 ults in increased levels of fucosylation and sialylation.

7 by first removing and then restoring PrP(Sc) sialylation.

8 reduced by desialylation and was enhanced by sialylation.

9 inflammatory activity even in the absence of sialylation.

10 e with beta1,3-linked galactose and variable sialylation.

11 quire an accurate assessment of glycoprotein sialylation.

12 ransferases and GOLPH3 was important for the sialylation.

13 greatly and in particular, with respect, to sialylation.

14 sted as potential inhibitors of cell surface sialylation.

15 ubules, with a concomitant increase in TRPV5 sialylation.

16 ged as a promising model for studying neural sialylation.

17 s on FcgammaRIIIA binding is their increased sialylation.

18 but, more specifically, those which contain sialylation.

19 rotein (fHbp), and lipooligosaccharide (LOS) sialylation.

20 e independently of lipooligosaccharide (LOS) sialylation.

21 and may act as tissue-specific regulators of sialylation.

22 I (HepI) chain of LOS and decreased with LOS sialylation.

23 and systolic BP was inversely related to IgG sialylation.

24 th minor alterations in overall cell surface sialylation.

25 ssociated with a marked reduction in protein sialylation, a process highly dependent on intralumenal

26 were enriched in SAA but had lower levels of sialylation across glycoproteins.

27 These data suggest a protective role of IgG sialylation against the development of cryoglobulin-medi

28 lation) and augmented by Asn(347) NeuAc-type sialylation (all p < 0.05).

29 cell surface glycans display an increase of sialylation alpha2-6, poly-LacNAc, and fucosylation, all

30 Recent evidence suggests that sialylation also affects the binding of chemokines to th

31 ng to this functionality, and high levels of sialylation also lead to longer serum retention times ad

32 This variation included terminal sialylation and ABO(H) blood group expression on VWF.

33 ST6Gal-I-high cells had greater alpha2-6 sialylation and activation of EGFR than ST6Gal-I-low cel

34 ly, we show that increased levels of protein sialylation and alpha-1,6-linked core fucosylation are o

35 itis but fail to support the significance of sialylation and basophil involvement in the mechanism of

36 s spectrometry, and the relationship between sialylation and BP was evaluated.

37 sensing platform for following and detecting sialylation and desialylation processes.

38 However, MOE only reports on global sialylation and dissected information regarding subsets

39 verexpression increased alpha2,6-linked CD31 sialylation and dose-dependently counteracted NEU1-media

40 Thus, IgG sialylation and endothelial FcgammaRIIB may represent pr

41 ables experimental investigations of defined sialylation and facilitates a rational design of glycan

42 While sialylation and fucosylation have predominated the focus

43 e, we discuss how Fc structures arising from sialylation and fucosylation impact immunity, focusing o

44 one-cell stage enables systematic imaging of sialylation and fucosylation in live zebrafish embryos a

45 ells, especially the sequences with terminal sialylation and fucosylation, and addition of LacNAc rep

46 in glycosylation, including N-glycosylation, sialylation and fucosylation, were observed between earl

47 ivity leads to a loss of isoform-specific Kv sialylation and function, thereby limiting Kv activity d

48 in the context of gene mutations that affect sialylation and galactosylation.

49 ted in all three cell populations, increased sialylation and increased core diversity characterized t

50 known regarding the in vivo regulation of Fc sialylation and its role in the progression of inflammat

51 The sialylation and LacNAc extension patterns of the three E

52 h different combinations of fucosylation and sialylation and performed side-by-side in vitro FcgammaR

53 mutant (L858R/T790M) had a higher degree of sialylation and phosphorylation at Y1068, Y1086, and Y11

54 recursor N-acetyl-D-mannosamine restored IgG sialylation and preserved insulin sensitivity without af

55 more, in HFD-fed mice, ManNAc normalized IgG sialylation and prevented obesity-induced hypertension.

56 Sialylation and sialic acid linkage in N-glycans are mar

57 genes and glycans revealed the importance of sialylation and sialylated glycans in breast cancer brai

58 N-acetylmannosamine (ManNAc) led to improved sialylation and survival of mutant pups beyond P3.

59 produce products with a consistent state of sialylation and, therefore, require an accurate assessme

60 are largely unaffected by adjacent internal sialylation, and in several cases the internal sialic ac

61 mplex and high mannose structures, increased sialylation, and increased alpha-Gal termination in the

62 ain glycosylation features: galactosylation, sialylation, and level of bisecting N-acetylglucosamine

63 ltered glycosaminoglycan, GABAergic synapse, sialylation, and purine metabolism pathways.

64 umans, obesity was associated with lower IgG sialylation, and systolic BP was inversely related to Ig

65 alylation is due to aberrant alpha2,8-linked sialylation, and the expression of three genes (ST8sia1,

66 ST activity with malignancy and cell surface sialylation, and the sialylation inhibition activity of

67 lines displayed TNFR1 with elevated alpha2-6 sialylation, and these cells were significantly protecte

68 Gal-I displayed TNFR1 with elevated alpha2-6 sialylation, and this was associated with diminished TNF

69 ST8sia4, and ST8sia6) that mediate alpha2,8 sialylation are downregulated in NKAP-defcient RTEs.

70 s that contribute to the cancer cell-surface sialylation are not well characterized, specifically in

71 stablish IgE glycosylation, and specifically sialylation, as an important regulator of allergic disea

72 was minimally sufficient to elicit extrinsic sialylation, as demonstrated with the FeCl3 model of mes

73 pha1-3,-4 fucosylation, but not the terminal sialylation, assists the binding of BCMA with ligands in

74 tic strategy relies on a chemical alpha(2,6)-sialylation at the internal GalNAc unit of a Gb5 pentasa

75 all three molecules were absent; again, LOS sialylation attenuated the AP in the absence of both fHb

76 ies of 6-19 IgG3 rheumatoid factor, terminal sialylation attenuated the nephritogenic potential of 6-

77 oter of the ST6Gal-1 gene is critical for Fc sialylation, but P1 does not drive ST6Gal-1 expression i

78 ed to the media of cultured cells shuts down sialylation by a mechanism involving its intracellular c

79 ular hyposialylation, and increasing overall sialylation by extrinsic sialic acid intake reduced ROS

80 oieties into the natural GlcNAc, followed by sialylation by sialyl transferases gave 12 differently f

81 d that the structural alterations induced by sialylation can be mimicked by specific amino acid modif

82 Rather, IgG sialylation can be regulated by the liver and platelets

83 Our study demonstrates that protein sialylation can be reliably and quantitatively character

84 blation of ST6Gal1 in mice revealed that IgG sialylation can occur in the extracellular environment o

85 support the hypothesis that loss of terminal sialylation contributes to the pathophysiology underpinn

86 Inhibitors of cell surface sialylation could be a useful tool in cancer, immune, an

87 ttern, we discovered that the enhanced rhEPO sialylation could be attributed to a decrease in neutral

88 This effect on sialylation could be reversed by the selective COX-2 inh

89 tions in their extent of galactosylation and sialylation could modulate IgG Fc-dependent effector fun

90 Accordingly, the sialylation defects progressed with time and paralleled

91 Sialylation-deficient Chinese hamster ovarian (CHO) epit

92 We generated a podocyte-specific sialylation-deficient mouse model (PCmas(-/-) ) by targe

93 SPR/Cas9 technology to generate immortalized sialylation-deficient podocytes (asialo-podocytes) for f

94 reports on the direct correlation between GG sialylation degree and brain developmental stage.

95 creting cells do not exclusively control the sialylation-dependent anti-inflammatory function of IgG.

96 Sialylation-dependent ligand recognition is also a prope

97 The current work tests a new hypothesis that sialylation determines the fate of prions in an organism

98 alylated IVIg drug candidate with maximum Fc sialylation devoid of unwanted alterations to the IVIg m

99 In contrast, in the absence of fucosylation, sialylation did not adversely impact ADCC.

100 tion of dsPMCAb-derived PrP(Sc) with reduced sialylation did not cause prion disease.

101 ports by others, we found that sulfation and sialylation drastically alter the MS(2) fragmentation pa

102 logical hurdles in producing defined protein sialylation due to the enormous structural diversity ren

103 231BR, which could result in an increase of sialylation during brain metastasis.

104 rmore, it was possible to detect an impaired sialylation during kidney maturation in a transgenic mou

105 Thus, sialylation during T cell maturation is critical to prot

106 st significant effect on the Y1173 site, the sialylation effect is not strong enough to stop cancer p

107 Our data suggest that sialylation enables recognition of endolyn by galectin-9

108 LOS sialylation enhanced binding of fH C-terminal domains 18

109 trated that lack of galactosylation, but not sialylation, enhanced the pathogenic activity of 34-3C I

110 LOS sialylation enhances complement resistance by recruiting

111 in excellent agreement with previous in vivo sialylation experiments.

112 >3 days, reiterating the importance of LNnT sialylation for infection.

113 In addition to exhibiting low levels of sialylation, GNE-deficient cells produced distinct N-lin

114 ascites or solid tumors sorted for alpha2-6 sialylation grew as spheroids, while cells lacking alpha

115 role of fragment crystallizable domain (Fc) sialylation has presented an opportunity to develop more

116 ations in this process, especially increased sialylation, have been associated with malignant transfo

117 Whether altered sialylation impairs nephrin function in human disease re

118 lthough the Cmas(nls) mouse displayed normal sialylation in all organs including kidney, a critical s

119 We examined the enzymatic basis for glycan sialylation in animal systems by determining the crystal

120 We also observed in vivo extrinsic sialylation in animals deficient in ST6Gal-1, demonstrat

121 n AP and broaden the molecular basis for LOS sialylation in AP regulation on meningococci in more tha

122 Strategies to target aberrant sialylation in cancer, however, have evolved comparative

123 -3-O-methyl-D-mannose decreases cell surface sialylation in Jurkat cells in a dose-dependent manner u

124 The role of sialylation in kidney biology is not fully understood.

125 To study sialylation in macrophages, we treated U937 monocytic ce

126 Loss of cell-surface sialylation in mice resulted in disturbance of podocyte

127 The role of altered sialylation in multiple myeloma (MM) cell trafficking ha

128 fferences in bisection, galactosylation, and sialylation in N-glycans derived from different sources

129 Progressive loss of sialylation in PCmas(-/-) mice resulted in onset of prot

130 xplanation for the role of terminal alpha2,6-sialylation in precluding the interaction of natural N-g

131 Here we observed no significant extrinsic sialylation in resting mice, suggesting that extrinsic s

132 The key importance of alpha2,3-sialylation in SnL expression was demonstrated by increa

133 Sialylation in the context of core fucosylation signific

134 However, extrinsic sialylation in the periphery could be triggered by infla

135 In addition, although sialylation in the TKI-resistant mutants suppresses EGFR

136 mination and relative quantitation of IgG Fc sialylation in therapeutic IgG samples.

137 Likewise, estimated variations in sialylation in tissue homogenates might be simply the re

138 tance of altered glycosylation, particularly sialylation, in MM cell adhesion and migration.

139 elative contributions of galactosylation and sialylation, in relation to cryoglobulin activity, to th

140 changes in N-glycosylation; reduced channel sialylation increases hERG channel activity during the a

141 OS sialylation, increasing abundance of LA d-glucosamine ve

142 We demonstrate that sialylation induces significant structural alterations i

143 However, LOS sialylation inhibited the rat AP and, as with human seru

144 gnancy and cell surface sialylation, and the sialylation inhibition activity of inhibitors.

145 udies in mice demonstrated that the level of sialylation is a major determinant of CSL040 clearance i

146 In summary, sialylation is critical during the development of the gl

147 We demonstrate that the defect in sialylation is due to aberrant alpha2,8-linked sialylati

148 sialylation of IgG Fc and that defective Fc sialylation is likely a major contributing mechanism for

149 In particular, N-linked sialylation is markedly reduced (>50%) compared with pla

150 n in resting mice, suggesting that extrinsic sialylation is not a constitutive process.

151 er, important information about glycopeptide sialylation is not duly covered because of in-source and

152 nt in ST6Gal-1, demonstrating that extrinsic sialylation is not mediated exclusively by ST6Gal-1.

153 ning central veins in the liver and that IgG sialylation is powered by serum-localized nucleotide sug

154 Protein sialylation is ubiquitous and essential in a wide range

155 ated experimental data and predicted reduced sialylation leads to a 50-70-ms decrease in action poten

156 Sialylation levels and patterns modulate recognition eve

157 -6)-linked sialic acids and had very similar sialylation levels as pd-A1PI.

158 we were able to accurately quantify relative sialylation levels of Erythropoietin.

159 Ab), and also generated PrP(Sc) with reduced sialylation levels using the same method but with partia

160 t received PrP(Sc) with original or restored sialylation levels were infected, whereas none of the an

161 Precise assignment of sialylation linkages at the glycopeptide level is of imp

162 Our data support the notion that loss of sialylation might be part of the complex process causing

163 s, rather than increased galactosylation and sialylation, modifies the Fc conformation(s) relevant fo

164 in complex and oligomannose type N-glycans, sialylation (mono-, di-, and tetra-), fucosylation (tri-

165 ylation critical quality attributes (GCQAs): sialylation, N-glycolyl-neuraminic acid (Neu5Gc) content

166 s under conditions of full glycosylation, no sialylation, no complex N-glycans, and following enzymat

167 Fc sialylation of a CD20-targeting antibody had no impact o

168 LOS sialylation of A2594 DeltafHbpDeltaNspA decreased the ra

169 The production and sialylation of AAT increased in COVID-19, but this antii

170 Our data suggest that sialylation of antigens provides an attractive way to in

171 lar to that of wild-type mice, with improved sialylation of both nephrin and podocalyxin, as well as

172 Together, these results indicate that sialylation of C. jejuni LOS increases DC activation and

173 c 6-phosphate and has a direct impact on the sialylation of cell surface components.

174 athogens from host cells, including terminal sialylation of cell surface glycans.

175 We found sialylation of cell surface was significantly increased

176 Here, we report that decreased Fc sialylation of circulatory IgG accompanies the acute pha

177 n ST6Gal-I substrate, and show that alpha2-6 sialylation of Fas confers protection against Fas-mediat

178 carcinoma cell models, we find that alpha2-6 sialylation of Fas prevents apoptosis stimulated by FasL

179 cytotoxicity (ADCC), while terminal alpha2,6-sialylation of Fc glycan plays a critical role for the a

180 Given that sialylation of Fc glycans decreases ADCC, one explanatio

181 yltransferase genes responsible for terminal sialylation of gangliosides and some glycoproteins.

182 5Ac) are considered a limiting factor in the sialylation of glycoproteins.

183 e marrow chimeras demonstrated that alpha2,6-sialylation of HSPCs is profoundly dependent on circulat

184 Here, we report that IgG Fc sialylation of human monoclonal IgG1 molecules impairs t

185 racellular ST6Gal-1 in the blood impacts the sialylation of IgG Fc and that defective Fc sialylation

186 expression of factor H binding protein, and sialylation of lipopolysaccharide, which are essential f

187 ve disease, these data strongly suggest that sialylation of LOS is dispensable for H. ducreyi pathoge

188 o differentiated cell types included greater sialylation of N-glycans in EBs, whereas alpha-Gal-cappe

189 HPLC and LC/MS analysis showed that the sialylation of N-glycans was specifically decreased in K

190 a together with EPO-Fc and the machinery for sialylation of N-glycans.

191 This study illustrates that sialylation of N-linked glycans creates a prion replicat

192 itical shortage of CMP-sialic acid prevented sialylation of nephrin and podocalyxin in the maturing p

193 Normal sialylation of plasma proteins was observed in spite of

194 We proposed that sialylation of PrP(Sc) is essential for evading innate i

195 Enhanced sialylation of PrP(Sc) is recapitulated in vitro by incu

196 ipid (GSL) biosynthesis, we can increase the sialylation of recombinant human erythropoietin (rhEPO)

197 rase expression, can critically modulate the sialylation of specific glycans while leaving others vir

198 ialic acid labeling indicated a reduction in sialylation of ST3Gal4(-/-) ventricular Kv4.2 and Kv1.5,

199 alic acid in extracellular ST6Gal-1-mediated sialylation of target cell surfaces.

200 Specifically, alpha2,6-sialylation of terminal LacNAc residues in the end buds

201 was successfully applied to characterize the sialylation of the apical region of epididymal epithelia

202 omeostatic apoptosis through alpha2,6-linked sialylation of the death receptor TNFR1 in many cell typ

203 2E, and V264E) increased galactosylation and sialylation of the Fc and, concomitantly, reduced the af

204 gammaRs but is independent of FcgammaRIIb or sialylation of the Fc fragment in the human setting.

205 Sialylation of the Fc fragment is mediated by beta-galac

206 The relative sialylation of the Fc glycan on mouse IgG, which influen

207 anti-inflammatory activity is attributed to sialylation of the Fc glycan.

208 ecific LOS modifications, including terminal sialylation of the LOS, which exploits host-derived sial

209 ctericidal IgM in serum and are protected by sialylation of the LOS.

210 Consequently, our study proves that sialylation of the N297 sugar moiety has a direct impact

211 inhibitor of GSL biosynthesis increases the sialylation of the rhEPO they produce.

212 These studies reveal that modifying sialylation of the soluble glycoprotein angiopoietin-lik

213 expression of ST3Gal-I, leading to increased sialylation of the substrate of ST3Gal-I, core 1 Galbeta

214 haride, suitable for an enzymatic alpha(2,3)-sialylation of the terminal Gal residue to construct a h

215 overexpression, we determined that alpha2-6 sialylation of TNFR1 had no effect on early TNF-induced

216 The alpha2-6 sialylation of TNFR1 was found to inhibit TNF-induced TN

217 Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and i

218 In contrast, the effect of sialylation on ADCC was dependent on the status of core

219 NKAP-deficient T cells have a defect in sialylation on cell surface glycans, leading to IgM recr

220 eport, we further investigated the effect of sialylation on the phosphorylation profile of EGFR in TK

221 Here we investigated the impact of reduced sialylation on ventricular repolarization through gene d

222 gh extracellular ST6Gal-1 supports extrinsic sialylation, other sialyltransferases are present in sys

223 tion of multigene vectors carrying the human sialylation pathway into glycosylation-destructed mutant

224 However, the sialylation pathway was not investigated in Drosophila b

225 Here, we report that the cellular sialylation pathway, as well as epigenetic reprogramming

226 oles that CMAS has in the nucleus and in the sialylation pathways of animal cells.

227 Existing analytical strategies to determine sialylation patterns are mostly applied to tissue sample

228 Second, because the alteration of sialylation patterns has been previously implicated in v

229 ialylated Abeta standards to reveal specific sialylation patterns of individual Abeta glycopeptides i

230 totoxicity (ADCC), whereas terminal alpha2,6-sialylation plays a critical role in the anti-inflammato

231 While sialylation plays important functions in the nervous sys

232 ntify new markers for oral cancer, we used a sialylation probe to investigate the glycoproteins diffe

233 of intracellular ST activity and tracing of sialylation process.

234 Our findings suggest that analyzing sialylation processes using this platform can become a u

235 ) as the chemoselective recognition probe of sialylation product, both of which are encapsulated in a

236 ouse models, ST6GAL1 from B cells alters the sialylation profile of bone marrow populations, and matu

237 Sialylation regulates the in vivo half-life of recombina

238 w as spheroids, while cells lacking alpha2-6 sialylation remained as single cells and lost viability.

239 LOS sialylation renders gonococci resistant to complement an

240 In vivo, loss of sialylation resulted in mislocalization of slit diaphrag

241 frequently capped by sialic acid (Sia), and sialylation's crucial role for kidney function is well k

242 an podocytes are highly sialylated; however, sialylation's role in podocyte homeostasis remains uncle

243 The sialylation showed selectivity toward the N-glycan compa

244 an keratinocytes, to localize hyaluronan and sialylation sites.

245 by restraining TNFR1 at the cell surface via sialylation, ST6Gal-I acts as a functional switch to div

246 Because the sialylation state of a receptor can influence its functi

247 l surface expresses multiple molecules whose sialylation state regulates multiple aspects of endothel

248 OS sialylation status but not LA structure showed significa

249 Alterations in the sialylation status in a distinct area of tissues or in a

250 To resolve this controversy, we analyzed the sialylation status of GPIs within PrP(Sc) generated in t

251 and GPI-anchored PrP(Sc) The question of the sialylation status of GPIs within PrP(Sc) has been contr

252 Instead, this work suggests that the sialylation status of GPIs within PrP(Sc) is regulated i

253 strates that, upon colonization of SLOs, the sialylation status of prions changes by host STs.

254 Sc) Furthermore, this work suggests that the sialylation status of PrP(Sc) plays an important role in

255 In this study, the sialylation status of PrP(Sc) was reduced by replicating

256 Here we show that the sialylation status of the infectious, disease-associated

257 ion to a wild type host is determined by the sialylation status of the inoculated PrP(Sc) Furthermore

258 PrPC molecules selectively, according to the sialylation status of their N-linked glycans.

259 Consequently, the cellular sialylation status under healthy and pathological condit

260 alitative and quantitative estimation of the sialylation status, sialic acids were released, fluoresc

261 racerebral administration depends on PrP(Sc) sialylation status.

262 g human kidney specimens based on glomerular sialylation status.

263 G-mediated cellular depletion, regardless of sialylation status.

264 mimic the structural alterations induced by sialylation, such as F241A, can be promising therapeutic

265 Sialylation, such as through a Sia supplementation thera

266 Finally, embryos devoid of cell surface sialylation suffered from malnutrition due to inadequate

267 Fc, a mutant that displays increased glycan sialylation, suggests that increased conformational flex

268 Previously, we demonstrated that EGFR sialylation suppresses its dimerization and phosphorylat

269 the human brain with a much higher degree of sialylation than previously reported.

270 r multimers leads to molecules with enhanced sialylation that may be suitable for managing inflammati

271 apsulation and intrinsic lipooligosaccharide sialylation that may promote adherence to mucosal surfac

272 Upon sialylation, the affinities for Fcgamma receptors are re

273 After enzymatic sialylation, the presence of sialic acid was confirmed u

274 ancer cell metastasis by decreasing N-glycan sialylation, thus altering the regulation of talin/integ

275 involves a highly alpha(2,3)-stereoselective sialylation to a galactose acceptor.

276 fined the contribution of galactosylation or sialylation to the pathogenic activity of IgG1 and IgG2a

277 ugh we cannot preclude a contribution of LOS sialylation to ulcerative disease, these data strongly s

278 For example, Fc domain sialylation triggers conformational changes of IgG1 that

279 lar endothelial surface undergoes changes in sialylation upon adopting the migratory/angiogenic pheno

280 s mediate tissue damage, induction of IgG Fc sialylation was associated with clinical disease remissi

281 The requirement for sialylation was examined in the collagen Ab-induced arth

282 A time dependent sialylation was followed by electrochemical impedance sp

283 PrP(Sc) sialylation was found to be critical for effective traff

284 Importantly, sialylation was highly specific to pathogenic IgG at the

285 However, Fc sialylation was increased 3-fold from the base line upon

286 Sialylation was induced in cancer progression to inhibit

287 IgG Fc glycan sialylation was interrogated in 3442 human participants

288 roscopy, was enzymatically desialylated, and sialylation was quantified by flow cytometry.

289 In particular, terminal alpha(2-6)-linked sialylation was reduced in patients with low VWF, with a

290 molecular layer roughness in response to the sialylation was visualized using atomic force microscopy

291 To prevent loss of alpha2-6 sialylation, we forced constitutive expression of ST6Gal

292 confirm the physiologic importance of TNFR1 sialylation, we generated overexpressing ST6Gal-I transg

293 ands (fHbp and NspA) and lipooligosaccharide sialylation were deleted in all strains.

294 Effects of normalizing IgG sialylation were determined in HFD-fed mice administered

295 e animals that received PrP(Sc) with reduced sialylation were infected.

296 cted to glomeruli, whereas no differences in sialylation were observed when whole kidney homogenates

297 Charge species due to deamidation and sialylation were separated by CZE.

298 tose residues per se or the lack of terminal sialylation, which is dependent on galactosylation.

299 Although blockade of sialylation with 3F-NeuAc does not affect viability of c

300 ha chains would enhance virulence, and their sialylation would enable gonococci to survive within pol