ライフサイエンスコーパス: snRNA

1 snRNA Psis are guided by single hairpin snoRNAs, also im

2 snRNA-seq achieves comparable gene detection to scRNA-se

3 NA (different subunits) and 5250 miRNA, 3747 snRNA, gene sequences from 9282 complete genome chromoso

4 n (snRNP), which contains, additionally, 7SK snRNA, methyl phosphate-capping enzyme (MePCE), and La-r

5 Depletion of 7SK snRNA or Larp7 disrupts LEC integrity, inhibits RNAPII r

6 The 7SK snRNA specifically associates with a fraction of RNAPII

7 We demonstrate that two abundant snRNAs, WsnRNA-46 and WsnRNA-49, are expressed in Wolbac

8 east, the U2 small nuclear ribonucleic acid (snRNA) component of the spliceosome is targeted for addi

9 riptional modification of U56 and U93 alters snRNA conformational dynamics by distinct mechanisms and

10 view that 21U RNA biogenesis is built on an snRNA-related pathway.

11 implications for understanding canonical and snRNA 3'-end processing.

12 ur in the 5' splice-site binding region, and snRNA-mutant tumours have significantly disrupted RNA sp

13 pression datasets with both bulk RNA-seq and snRNA-seq data, Bisque replicates previously reported as

14 scRNA-Seq and snRNA-Seq from matched samples recovered the same cell t

15 n clinical tumor samples using scRNA-Seq and snRNA-Seq, respectively.

16 iety of noncoding RNAs-snoRNAs, scaRNAs, and snRNAs-that are dependent on Cajal bodies for stability

17 ence for small RNA genes (tRNAs, snoRNAs and snRNAs) suggesting a putative role for RNA in its recrui

18 RNAs), and alters the occupancy of Pol II at snRNA loci.

19 ther, our results draw new parallels between snRNA and piRNA biogenesis in nematodes and provide evid

20 In addition, DSP1 binds snRNA loci and interacts with Pol-II in a DNA/RNA-depend

21 The analysis revealed 42 Psis on T. brucei snRNAs, which is the highest number reported so far.

22 Analysis by snRNA-seq identified transcript profiles and inferred fu

23 least four additional proteins, to catalyze snRNA 3' end maturation in Arabidopsis.

24 Here, we identified and characterized snRNAs from the endosymbiotic bacteria, Wolbachia, which

25 We then compared snRNA-seq of myoblasts before and after differentiation.

26 By contrast, snRNA-seq from all three platforms captured a diversity

27 ived from core promoter elements controlling snRNA transcription.

28 o increases the levels of assembly-defective snRNAs and suppresses some splicing defects seen in SMN-

29 To determine how assembly-defective snRNAs are degraded, we first demonstrate that yeast U1

30 antling of the Prp3-binding site on U4/U6 di-snRNA but leaves the Prp31- and Snu13-binding sites on U

31 In solution, the U4/U6 di-snRNA forms a 3-helix junction with a planar Y-shaped st

32 The U4/U6 di-snRNA is conserved in eukaryotes and is part of the U4/U

33 sing factor 31 (Prp31), and Prp3 to U4/U6 di-snRNA leads to a stepwise decrease of Brr2-mediated U4/U

34 i-snRNAs and inhibits Brr2-mediated U4/U6 di-snRNA unwinding in vitro.

35 yses that Prp3 contains a bipartite U4/U6 di-snRNA-binding region comprising an expanded ferredoxin-l

36 f a 92-nt 3-helix junction from the U4/U6 di-snRNA.

37 operatively with Snu13 and Prp31 on U4/U6 di-snRNAs and inhibits Brr2-mediated U4/U6 di-snRNA unwindi

38 strongly inhibited by mutations in U4/U6 di-snRNAs that diminish the ability of U6 snRNA to adopt an

39 the spliceosome from unstable genome-encoded snRNA variants.

40 s ptRNA-subclasses that exist in eukaryotes: snRNA, snoRNA, RNase P, RNase MRP, Y RNA or telomerase R

41 It consists of five snRNAs and more than 200 proteins.

42 Although DIEM was designed for snRNA-seq, our clustering strategy also successfully fil

43 subunits, which are largely dispensable for snRNA processing, also have regulatory roles at these pr

44 CESSING 1 (DSP1) is an essential protein for snRNA 3' end maturation in Arabidopsis.

45 de evidence for the production of functional snRNAs by Wolbachia that play roles in cross-kingdom com

46 metal cofactors of the spliceosome alter how snRNAs respond to these modifications.

47 1 and U2 genes as models, we show that human snRNA genes are more similar to mRNA genes than yeast sn

48 Thus, human snRNA genes may use chromatin structure as an additional

49 Production of snR-DPGs required the Pol II snRNA promoter (PIIsnR), and CPL4RNAi plants showed incr

50 s a previously unknown function of CPSF73 in snRNA maturation.

51 , reflecting a strong and specific defect in snRNA 3'-end formation.

52 Here, we show that DEFECTIVE in snRNA PROCESSING 1 (DSP1) is an essential protein for sn

53 uantify contamination and filter droplets in snRNA-seq experiments, called Debris Identification usin

54 r7, which is a hallmark of RNAPII engaged in snRNA synthesis.

55 a Hypoplasia Type 7 (PCH7) and implicated in snRNA and hTR processing.

56 snoRNA species implicated in snRNA pseudouridylation were identified by a genome-wide

57 nteraction is crucial for the role of INT in snRNA 3'-end processing.

58 chment for long non-coding RNAs (lncRNAs) in snRNA-seq.

59 were particularly sensitive to variations in snRNA abundance in a breast cancer cell line model were

60 As with highly structured 3' ends, including snRNAs and histone mRNAs, are naturally resistant to RNa

61 of non-polyadenylated transcripts including snRNAs and mRNAs encoding replication-dependent histone

62 ogically relevant perturbation of individual snRNAs drove widespread gene-specific differences in alt

63 nd stability of regular snRNAs while leaving snRNA variants unprocessed and exposed to degradation in

64 A turnover at short transcription units like snRNA-, replication-dependent histone-, promoter upstrea

65 identified the presence of expressed U1-like snRNAs in multiple species, including humans.

66 monstrated that a new class of human U1-like snRNAs, the variant (v)U1 snRNAs (vU1s), also participat

67 d noncoding RNAs (e.g., mRNA, miRNA, lncRNA, snRNA, tRNA, yRNA), DNA, proteins, and enzymes.

68 ere, we present an approach for multiplexing snRNA-seq, using sample-barcoded antibodies to uniquely

69 we first demonstrate that yeast U1 Sm-mutant snRNAs are degraded either by Rrp6- or by Dcp2-dependent

70 pecific sn/snoRNA genes, and reduces nascent snRNA and snoRNA synthesis.

71 operate to protect and chaperone the nascent snRNA during its journey to the spliceosome.The mechanis

72 for introns and various RNA classes (ncRNA, snRNA, snoRNA) and less variability after degradation.

73 single cells (scRNA-seq) and single nuclei (snRNA-seq) and found them comparable, with a distinct en

74 rocessing of snRNAs, increases the levels of snRNA primary transcripts (pre-snRNAs), and alters the o

75 se results identify a conserved mechanism of snRNA quality control, and also suggest a general paradi

76 r a key factor involved in the processing of snRNA 3' ends.

77 ecreases the occupancy of LEC at a subset of snRNA genes and results in a reduction in their transcri

78 ole in the recruitment of LEC to a subset of snRNA genes through direct interaction of EAF and the N-

79 ongation during transcription of a subset of snRNA genes.

80 However, levels of snRNAs did not follow the expression of splicing protein

81 ding to snRNAs are known to reduce levels of snRNAs, suggesting an unknown quality control system for

82 The biogenesis of the majority of snRNAs involves 3' end endonucleolytic cleavage of the n

83 plex was sufficient for 3'-end maturation of snRNAs.

84 al defects, impairs the 3' end processing of snRNAs, increases the levels of snRNA primary transcript

85 ulated pre-mRNA processing requires study of snRNAs, as well as protein splicing factors.

86 RNAi compromised the guided modification on snRNA and reduced parasite growth at elevated temperatur

87 t of the snRNP code to which Gemin5 binds on snRNAs.

88 otein WDR79, is essential for guiding Psi on snRNAs but not on rRNAs.

89 s restore snRNP assembly of Sm proteins onto snRNA and completely rescue both survival of Smn null mi

90 faithful delivery of seven Sm proteins onto snRNA and the formation of the common core of snRNPs.

91 utations did not affect pre-mRNA splicing or snRNA levels.

92 an cells lack antisense elements to rRNAs or snRNAs; thus, their targets remain unknown.

93 Our snRNA-seq protocol yielded 20-fold more podocytes compar

94 and a few other small RNA types like piRNA, snRNA and snoRNA.

95 complex with the Sm site or m(7)G cap of pre-snRNA, which reveal that the WD40 domain of Gemin5 recog

96 vels of TOE1 accumulated 3'-end-extended pre-snRNAs, and the immunoisolated TOE1 complex was sufficie

97 nd sufficient for binding the Sm site of pre-snRNAs by isothermal titration calorimetry (ITC) and mut

98 recognizes the Sm site and m(7)G cap of pre-snRNAs via two distinct binding sites by respective base

99 for the assembly of the ring complex on pre-snRNAs at the conserved Sm site [A(U)4-6G].

100 complex, is responsible for recognizing pre-snRNAs.

101 complex delivers pre-small nuclear RNAs (pre-snRNAs) to the heptameric Sm ring for the assembly of th

102 These pre-snRNAs contained 3' genome-encoded tails often followed

103 the levels of snRNA primary transcripts (pre-snRNAs), and alters the occupancy of Pol II at snRNA loc

104 5 in escorting the truncated forms of U1 pre-snRNAs for proper disposal.

105 ent CPSF73-I-containing complexes to process snRNAs and pre-mRNAs.

106 promotes maturation and stability of regular snRNAs while leaving snRNA variants unprocessed and expo

107 The small nuclear RNA (snRNA) activating protein complex (SNAPc) is essential f

108 hese data suggest that U6 small nuclear RNA (snRNA) and RtcB participate in the formation of chimeric

109 The small nuclear RNA (snRNA) components of the spliceosome undergo many confor

110 ption of Pol II-dependent small nuclear RNA (snRNA) genes.

111 ncluding c-myc and LEC to small nuclear RNA (snRNA) genes.

112 Yeast U2 small nuclear RNA (snRNA) nucleotides that form base pairs with the branch

113 Mg(2+) site in the U2/U6 small nuclear RNA (snRNA) triplex, and the 5'-phosphate of the intron nucle

114 mal complex comprising U5 small nuclear RNA (snRNA), extensively base-paired U4/U6 snRNAs and more th

115 RNP), composed of the 7SK small nuclear RNA (snRNA), MePCE, and Larp7, regulates the mRNA elongation

116 e for the U6 spliceosomal small nuclear RNA (snRNA).

117 atalytic metal site in U6 small nuclear RNA (snRNA).

118 authentic pre-mRNA and U7 small nuclear RNA (snRNA).

119 stemloops in U1 and/or U2 small nuclear RNA (snRNA).

120 on a functional U2 snRNP (small nuclear RNA [snRNA] plus associated proteins), as H2A.Z shows extensi

121 l nucleolar RNA [snoRNA], small nuclear RNA [snRNA], small Cajal body-specific RNA [scaRNA], and tran

122 xpression of modified U1 small nuclear RNAs (snRNA) complementary to the splice donor sites strongly

123 ly downstream from viral small nuclear RNAs (snRNA).

124 In prokaryotes, small noncoding RNAs (snRNAs) of 50-500 nt are produced that are important in

125 tion of 3'-extensions of small nuclear RNAs (snRNAs) and biogenesis of novel transcripts from protein

126 Uridine-rich small nuclear RNAs (snRNAs) are the basal components of the spliceosome and

127 3A>G) of U1 spliceosomal small nuclear RNAs (snRNAs) in about 50% of Sonic hedgehog (SHH) medulloblas

128 hat TOE1 associated with small nuclear RNAs (snRNAs) incompletely processed spliceosomal.

129 host RNAs, particularly small nuclear RNAs (snRNAs), and avoidance of host transcripts encoding host

130 , transfer RNAs (tRNAs), small nuclear RNAs (snRNAs), and RMRP.

131 rsors to specific tRNAs, small nuclear RNAs (snRNAs), and small nucleolar RNAs (snoRNAs) are all enri

132 in 3' end processing of small nuclear RNAs (snRNAs), attenuates MtnA transcription during copper str

133 methylation of rRNAs and small nuclear RNAs (snRNAs), respectively.

134 Although these small nuclear RNAs (snRNAs), termed U1, U2, U4, U5, and U6 snRNA, are presen

135 sis) on the spliceosomal small nuclear RNAs (snRNAs), which may enable growth at the very different t

136 compartments enriched in small nuclear RNAs (snRNAs)-and promotes efficient spliceosomal snRNP assemb

137 oteins plus U4/U6 and U5 small nuclear RNAs (snRNAs).

138 As such as small nuclear and nucleolar RNAs (snRNAs and snoRNAs).

139 A-seq (scRNA-seq) or single-nucleus RNA-seq (snRNA-seq) data to generate a reference expression profi

140 Single-nucleus RNA-seq (snRNA-seq) enables the interrogation of cellular states

141 d from fresh tumors, single-nucleus RNA-Seq (snRNA-Seq) is needed to profile frozen or hard-to-dissoc

142 Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue,

143 ghts, we used single nuclear RNA sequencing (snRNA-seq) and translating ribosome affinity purificatio

144 by performing single nuclei RNA sequencing (snRNA-seq) at multiple stages of mouse embryonic develop

145 Single-nucleus RNA sequencing (snRNA-seq) measures gene expression in individual nuclei

146 roplet-based, single-nucleus RNA sequencing (snRNA-seq) of A1 across three developmental time points

147 rmed unbiased single-nucleus RNA sequencing (snRNA-seq) on cryopreserved human diabetic kidney sample

148 We utilized single nucleus RNA sequencing (snRNA-seq) to examine the transcriptomes of over 16 000

149 platform with single-nucleus RNA sequencing (snRNA-seq) using sNuc-DropSeq, DroNc-seq, and 10X Chromi

150 Here we use single-nucleus RNA-sequencing (snRNA-seq) analysis in mice and humans to characterize a

151 whole-cell and single-nuclei RNA-sequencing (snRNA-seq) methods, here we show that snRNA-seq faithful

152 , we utilized single-nucleus RNA-sequencing (snRNA-seq) to determine the extent of transcriptional di

153 otein-coding genes downstream of the snRNAs (snRNA-downstream protein-coding genes [snR-DPGs]).

154 irst high-throughput mapping of spliceosomal snRNA Psis by small RNA Psi-seq.

155 ranscription of RNAPII-specific spliceosomal snRNA and small nucleolar RNA (snoRNA) genes.

156 In summary, snRNA-seq of activated neurons enables the examination o

157 hat the Integrator complex, which terminates snRNA transcription, is recruited to piRNA loci.

158 Our study demonstrates that snRNA-seq provides reliable transcriptome quantification

159 Our results further indicate that snRNA-seq has unique advantage in capturing nucleus-enri

160 We observe that snRNA-seq is commonly subject to contamination by high a

161 ncing (snRNA-seq) methods, here we show that snRNA-seq faithfully recapitulates transcriptional patte

162 udy identifies a complex responsible for the snRNA 3' end maturation in plants and uncovers a previou

163 We show here that Gemin5, the snRNA-binding protein of the SMN complex, binds directly

164 In the wild type, salt stress induced the snRNA-to-snR-DPG switch, which was associated with alter

165 plants showed increased read-through of the snRNA 3'-end processing signal, leading to continuation

166 tinuation of transcription downstream of the snRNA gene.

167 ell Integrator complex, which recognizes the snRNA 3' end processing signal (3' box), generates the 5

168 ive binding of all protein components to the snRNA duplex during di-snRNP assembly by electrophoretic

169 l for transcription of genes that encode the snRNAs.

170 from protein-coding genes downstream of the snRNAs (snRNA-downstream protein-coding genes [snR-DPGs]

171 ar splicing speckles and associates with the snRNAs that are involved in splice site recognition.

172 We evaluated DIEM using three snRNA-seq data sets: (1) human differentiating preadipoc

173 Surprisingly, in contrast to snRNA 3' end processing, HVS pre-miRNA 3' end processing

174 tment of Integrator or Heat Labile Factor to snRNA or RDH genes, respectively.

175 se of a neurodegenerative syndrome linked to snRNA maturation and uncover a key factor involved in th

176 Defects in Sm protein complex binding to snRNAs are known to reduce levels of snRNAs, suggesting

177 of all regular RNA polymerase II transcribed snRNAs of the major and minor spliceosomes by removing p

178 plants, suggesting that the transcriptional snRNA-to-snR-DPG switch may be a ubiquitous mechanism to

179 erns similar to canonical ncRNAs (e.g. tRNA, snRNA, miRNA, etc) on approximately 70% of human long nc

180 nctional RNA molecules including rRNA, tRNA, snRNA and ribozymes.

181 ites and modest delays at some histone and U snRNA genes, suggesting that the torpedo mechanism is no

182 omplex, to processing bodies, thus forming U snRNA bodies (U bodies).

183 , and Listeria interfere with spliceosomal U snRNA maturation in the cytosol.

184 to the decline in the cytosolic levels of U snRNAs and of the SMN complex proteins SMN and DDX20 tha

185 ial infection resulted in the rerouting of U snRNAs and their cytoplasmic escort, the survival motor

186 Mechanistically, targeting of U snRNAs to U bodies was regulated by translation initiati

187 than an overall reduction in Uridyl-rich (U)-snRNAs, may contribute to the specific neuromuscular dis

188 Strikingly, we have been unable to find a U1 snRNA candidate or any predicted U1-associated proteins,

189 esigned several U1 snRNA vectors to adapt U1 snRNA binding sequences of the mutated DDC gene.

190 Therefore, mutation-adapted U1 snRNA gene therapy can be a promising method to treat ge

191 e U1 snRNP core particle (Sm proteins and U1 snRNA), but not the mature U1 snRNP-specific proteins (U

192 ins to a lesser extent than the canonical U1 snRNA.

193 at ALS-associated FUS aberrantly contacts U1 snRNA at the Sm site with its zinc finger and traps snRN

194 gether, these data suggest that the human U1 snRNA variants analyzed here are unable to efficiently b

195 we identified these hotspot mutations in U1 snRNA in only <0.1% of 2,442 cancers, across 36 other tu

196 s the 3' introns, compensatory changes in U1 snRNA rescue trans-splicing of TSA mutants, demonstratin

197 We found that only the modified U1 snRNA (IVS-AAA) that completely matched both the introni

198 Alternative splicing mediated by mutant U1 snRNA inactivates tumour-suppressor genes (PTCH1) and ac

199 omplex (CBC) to the 5' end of the nascent U1 snRNA, which ultimately influences the utilization of U1

200 e saw reciprocal changes in the levels of U1 snRNA and U1 snRNP proteins.

201 splice sites, and exhibit high levels of U1 snRNA binding compared with cytoplasm-localized messenge

202 rpholino that base-pairs to the 5' end of U1 snRNA blocks splicing in the coupled system and complete

203 tion of U5, U6 snRNAs and accumulation of U1 snRNA in the B(act) complex.

204 Acute depletion of U1 snRNA or of the U1 snRNP protein component SNRNP70 marke

205 duplex between pre-mRNA and the 5'-end of U1 snRNA.

206 removal, although MBNL1 has no effect on U1 snRNA recruitment.

207 In this study, we designed several U1 snRNA vectors to adapt U1 snRNA binding sequences of the

208 U1 snRNP inserts the 5'SS-U1 snRNA helix between the two RecA domains of the Prp28 DE

209 We find that U1 snRNA is the primary RNA target of FUS via its interacti

210 ticles (snRNPs) that are comprised of the U1 snRNA and 10 core components, including U1A, U1-70K, U1C

211 e found that this interaction between the U1 snRNA and SF3A1 occurs within prespliceosomal complexes

212 The U1 snRNA is highly conserved across a wide range of taxa; h

213 the AU dincucleotide at the 5'-end of the U1 snRNA is highly conserved, despite the absence of an app

214 Thus, SL4 of the U1 snRNA is important for splicing, and its interaction wit

215 The U1 snRNA mutations occur in the 5' splice-site binding regi

216 eraction between stem-loop 4 (SL4) of the U1 snRNA, which recognizes the 5' splice site, and a compon

217 site of a pre-mRNA and the 5' end of the U1 snRNA.

218 These U1 snRNA mutations provide an example of highly recurrent a

219 rt defined by sequence complementarity to U1 snRNA, we identify RNA secondary structural elements nea

220 ed predominantly through basepairing with U1 snRNA whilst U1-C fine-tunes relative affinities of mism

221 (snRNP) through strong base-pairing with U1 snRNA.

222 U1 snRNAs associate with 5' splice sites in the form of rib

223 We show that Cr1-activating engineered U1 snRNAs (eU1s) have the unique ability to reprogram pre-m

224 Compensatory modified U1 snRNAs, complementary to mutated donor splice sites, wer

225 one case, we also evaluated exon-specific U1 snRNAs that, by targeting nonconserved intronic sequence

226 s of human U1-like snRNAs, the variant (v)U1 snRNAs (vU1s), also participate in pre-mRNA processing e

227 terminal RNA recognition motif of p65, a U12 snRNA binding protein, also binds to the distal 3' stem-

228 p65 protein-binding apical stem-loop of U12 snRNA can be replaced by this U6atac distal 3' stem-loop

229 ecular helix I region between U6atac and U12 snRNAs, several other regions within these RNA molecules

230 icates that Prp5 has reduced affinity for U2 snRNA that lacks Psi42 and Psi44 and that Prp5 ATPase ac

231 stem-loop (BSL)(8), but whether the human U2 snRNA folds in a similar manner is unknown.

232 er, our results indicate that the Psis in U2 snRNA contribute to pre-mRNA splicing by directly alteri

233 me formation was blocked by a mutation in U2 snRNA.

234 hens U4/U6 RNA-RNA and U2B"/U2A' proteins-U2 snRNA interaction at elevated temperatures.

235 intaining interactions with the keto-rich U2 snRNA.

236 s open conformation in U2 snRNP, and that U2 snRNA forms a BSL that is sandwiched between PRP5, TAT-S

237 iometric association of U2 snRNPs and the U2 snRNA is base-paired to the pre-mRNA.

238 U6 folds with U2 snRNA into an RNA-based active site that positions the 5

239 Yeast U2 snRNA contains three conserved Psis (Psi35, Psi42, and P

240 k-turns from ribosomes, riboswitches and U4 snRNA, finding a strong conservation of properties for a

241 d predispose them to ion-induced folding, U4 snRNA are strongly biased to an inability to such foldin

242 snRNA triggers unwinding of U6 snRNA from U4 snRNA.

243 ase relocation to its loading sequence in U4 snRNA, enabling Brr2 to unwind the U4/U6 snRNA duplex to

244 The single-stranded region of U4 snRNA between its 3' stem-loop and the U4/U6 snRNA stem

245 ves the Prp31- and Snu13-binding sites on U4 snRNA unaffected.

246 nu13-U4/U6 RNP into an intact Prp31-Snu13-U4 snRNA particle, free Prp3, and free U6 snRNA.

247 ffold during the entire assembly, but the U4 snRNA 5' stem-loop adopts alternative orientations each

248 anslocates only a limited distance on the U4 snRNA strand and does not actively release RNA-bound pro

249 ation via ATP-driven translocation on the U4 snRNA strand.

250 interact with the exon binding loop 1 of U5 snRNA.

251 he amino-terminal domain of Prp8 position U5 snRNA to insert its loop I, which aligns the exons for s

252 mutations in PRP16, PRP8, SNU114 and the U5 snRNA that affect this process interact genetically with

253 l and linker domains, and base-pairs with U5 snRNA loop 1.

254 U6 snRNA is transcribed by RNA polymerase III (Pol III) and

255 U6 snRNA undergoes post-transcriptional 3' end modification

256 to unwind the U4/U6 snRNA duplex to allow U6 snRNA to form the catalytic center of the spliceosome.

257 ruitment, packaging of both pre-tRNAs and U6 snRNA requires the nuclear export receptor Exportin-5.

258 RNAs (snRNAs), termed U1, U2, U4, U5, and U6 snRNA, are present in equal stoichiometry within the spl

259 lic phosphates at the ends of 5S rRNA and U6 snRNA.

260 e observations suggest that the conserved U6 snRNA methyltransferase evolved an additional function i

261 of the same gRNA expressed from different U6 snRNA promoters, with the previously untested U6:3 promo

262 then completed by the partially displaced U6 snRNA adopting an alternative conformation, which leads

263 13-U4 snRNA particle, free Prp3, and free U6 snRNA.

264 from U1 to U6 snRNA triggers unwinding of U6 snRNA from U4 snRNA.

265 U6 di-snRNAs that diminish the ability of U6 snRNA to adopt an alternative conformation but leave the

266 e fold, which recognizes a 3'-overhang of U6 snRNA, and a preceding peptide, which binds U4/U6 stem I

267 The invariant ACAGAGA sequence of U6 snRNA, which base-pairs with the 5'-splice site during c

268 the tri-snRNP and comparison with a Prp24-U6 snRNA recycling complex suggests how Prp3 may be involve

269 can activate the spliceosome by stripping U6 snRNA of all precatalytic binding partners, while minimi

270 he pairing of the 5' splice site with the U6 snRNA ACAGAGA region.

271 n nucleotides +3 to +6 base-pair with the U6 snRNA ACAGAGA sequence.

272 While the U6 snRNA catalytic core remains firmly held in the active s

273 yeasts rely on hyperstabilization of the U6 snRNA-5' splice site interaction to impede the 2nd step

274 r of the 5' splice site (5'SS) from U1 to U6 snRNA triggers unwinding of U6 snRNA from U4 snRNA.

275 t one function of Cwc2 is to stabilize U2-U6 snRNA helix I during splicing.

276 ju2 and Cwc25 as well as destabilizing U2-U6 snRNA helix I.

277 e ability of Delta247-Brr2 to bind the U4/U6 snRNA duplex at high pH and increases Delta247-Brr2's RN

278 ependent ATPase required to unwind the U4/U6 snRNA duplex during spliceosome assembly.

279 U4 snRNA, enabling Brr2 to unwind the U4/U6 snRNA duplex to allow U6 snRNA to form the catalytic cen

280 snRNA between its 3' stem-loop and the U4/U6 snRNA stem I is loaded into the Brr2 helicase active sit

281 n Prp16 stabilizes Cwc2 interactions with U6 snRNA and destabilizes Cwc2 interactions with pre-mRNA,

282 The U4 and U6 snRNAs are incorporated into the spliceosome as a base-p

283 The U2, U4, U5, and U6 snRNAs contain expected conserved sequences and have the

284 Base-pairing of U4 and U6 snRNAs during di-snRNP assembly requires large-scale rem

285 P contains extensively base paired U4 and U6 snRNAs, Snu13, Prp31, Prp3 and Prp4, seven Sm and seven

286 r RNA (snRNA), extensively base-paired U4/U6 snRNAs and more than 30 proteins, including the key comp

287 n, leading to a dramatic reduction of U5, U6 snRNAs and accumulation of U1 snRNA in the B(act) comple

288 ggered by unwinding of the U4 and U6 (U4/U6) snRNAs.

289 rated that the 3' stem-loop region of U6atac snRNA contains a U12-dependent spliceosome-specific targ

290 of various regions in the Sm proteins and U7 snRNA in 3' end processing of histone pre-mRNAs.

291 proof of concept of using the engineered U7 snRNA lentiviral vector for treatment of beta-thalassaem

292 of two core components: a ~60-nucleotide U7 snRNA and a ring of seven proteins, with Lsm10 and Lsm11

293 g these noncoding RNAs, regulation of the U7 snRNA by tRF-GG modulates heterochromatin-mediated trans

294 trate long-term splicing correction using U7 snRNA lentiviral vectors engineered to target several pr

295 A double-target engineered U7 snRNAs targeted to the cryptic branch point and an exoni

296 We validated snRNA-seq on fibrotic kidney from mice 14 days after uni

297 pressed, it is unclear whether these variant snRNAs have the capacity to form snRNPs and participate

298 little to no maturation of tested U1 variant snRNAs, which are instead targeted by the nuclear exosom

299 s host RNAs, including those associated with snRNA transcription, and avoidance of host transcripts e

300 omal activation through its interaction with snRNAs and possibly other spliceosomal proteins, reveali

301 es are more similar to mRNA genes than yeast snRNA genes with respect to termination.