ライフサイエンスコーパス: snRNP

1 nome-wide studies revealed that KAP1 and 7SK snRNP co-occupy most promoter-proximal regions containin

2 f an unprecedented mechanism controlling 7SK snRNP delivery to promoter-proximal regions to facilitat

3 pathway, calpain 2 cleavage of the core 7SK snRNP component MePCE promoted P-TEFb release and conseq

4 P7 and MEPCE to reconstruct a functional 7SK snRNP in vitro.

5 Here, we demonstrate that the human 7SK snRNP also functions as a canonical transcription factor

6 oop, liberating P-TEFb from the inactive 7SK snRNP, and inducing the formation of the Tat-SEC complex

7 on factor b (P-TEFb) from its inhibitory 7SK snRNP.

8 ed binding of RBM7 with core subunits of 7SK snRNP.

9 ory 7SK small nuclear ribonucleoprotein (7SK snRNP).

10 Fb are sequestered in the inactive-state 7SK snRNP complex.

11 ARP7) is a constitutive component of the 7SK snRNP and localizes to the 3' terminus of the 7SK long n

12 on elongation factor b (P-TEFb) from the 7SK snRNP in a manner that is dependent on its helicase acti

13 elongation and sn/snoRNA synthesis, the 7SK snRNP is a key regulator of nuclear RNA production by RN

14 ever, it remains unclear how and why the 7SK snRNP is assembled at these sites.

15 Here, we show that the assembly of the 7SK snRNP is preceded by an intermediate complex between HEX

16 is study, the release of P-TEFb from the 7SK snRNP led to increased synthesis of HEXIM1 but not HEXIM

17 ed enrichments for all components of the 7SK snRNP on RNAPII-specific sn/snoRNA genes.

18 es the interaction between PPM1G and the 7SK snRNP through site-specific PPM1G phosphorylation.

19 regulator KAP1 continuously tethers the 7SK snRNP to PRG promoters to facilitate P-TEFb recruitment

20 1 once P-TEFb has been released from the 7SK snRNP.

21 o intramolecular interactions within the 7SK snRNP.

22 f the kinase active P-TEFb from Hsp90 to 7SK snRNP for its suppression.

23 lacking exon 2B can rescue iMEF survival and snRNP assembly in the absence of flwt-Smn, indicating ex

24 r contribute to the biogenesis of miRNAs and snRNPs.

25 ted WT mice showed elevated anti-dsDNA, anti-snRNP, CXCL1, and MCP-1 levels compared to untreated mic

26 ed to untreated mice; however levels of anti-snRNP, MCP-1, and CXCL1 were reduced in pristane-treated

27 ts that have been characterized thus far are snRNP proteins because, in contrast to individual snRNPs

28 proliferating cells, which actively assemble snRNPs, as a readout for unperturbed SMN complex functio

29 d intracellular localization of autoantibody snRNP complexes was measured by flow cytometry and confo

30 low abundance minor spliceosome's catalytic snRNP, U6atac, is strikingly unstable (t(1/2)<2 hr).

31 ith both in vitro-reconstituted and cellular snRNPs led to similar changes in SHAPE reactivities, con

32 U1 small nuclear ribonucleoprotein complex (snRNP) control male courtship behavior.

33 e small nuclear ribonucleoprotein complexes (snRNPs) U2, U5 and U6 and the so-called NineTeen complex

34 y, SMA severity is correlated with decreased snRNP assembly activity.

35 /U6 di-small nuclear RNA-protein complex (di-snRNP) during spliceosome activation via ATP-driven tran

36 ein components to the snRNA duplex during di-snRNP assembly by electrophoretic mobility shift assay a

37 Base-pairing of U4 and U6 snRNAs during di-snRNP assembly requires large-scale remodeling of RNA st

38 of human RNPC3, also known as the U11/U12 di-snRNP 65-kDa protein, a unique component of the U12-type

39 in vivo results in accumulation of U4/U6 di-snRNP and impairs yeast growth.

40 6 helix II and an interface between U4/U6 di-snRNP and the U2 snRNP SF3b-containing domain, which als

41 The spliceosomal U4/U6 di-snRNP contains extensively base paired U4 and U6 snRNAs,

42 set up a recombinant Brr2-mediated U4/U6 di-snRNP disruption system, showing that sequential additio

43 of the function played by SART3 in U4/U6 di-snRNP formation, our discovery points to a direct link b

44 inimizing logistic requirements for U4/U6 di-snRNP reassembly after splicing.

45 Prp3 is an essential U4/U6 di-snRNP-associated protein whose functions and molecular m

46 ibes our hypothesis that recently discovered snRNPs functioned in pre-mRNA splicing.

47 tion and decreased recruitment of downstream snRNPs to nascent RNA.

48 roline-rich C-terminal tail of the essential snRNP core proteins SmN/B/B'.

49 Fruit fly snRNPs also fail to bind Ketel; however, the importin-7

50 ly identified human U1-like variants to form snRNPs and bind to U1 snRNP proteins.

51 ese variant snRNAs have the capacity to form snRNPs and participate in splicing.

52 SMN disrupts CB integrity and likely impairs snRNP maturation.

53 hich is important for its proper function in snRNP assembly.

54 orm scaRNPs, which play an important role in snRNP formation.

55 proteins because, in contrast to individual snRNPs, purification of intact spliceosomes has not been

56 the ability of the variants to assemble into snRNPs.

57 PN1, which is essential for TMG cap-mediated snRNP import in humans, is not well conserved in flies.

58 ges of SMN give rise to loss of SMN-mediated snRNP assembly and support the hypothesis that this loss

59 nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins.

60 Here, we characterize the non-snRNP PRP19 complex of Trypanosoma brucei.

61 include trafficking of mRNA and assembly of snRNP complexes.

62 lfils essential functions in the assembly of snRNPs, which are key components in the splicing of pre-

63 pensable protein essential for biogenesis of snRNPs, key components of pre-mRNA processing.

64 nRNA and the formation of the common core of snRNPs.

65 le of cells subjected to knockdown of Gro or snRNP-U1-C (a component of U1 snRNP) showed a significan

66 er tri-snRNP complex proteins, but not other snRNP spliceosome complexes, selectively abrogated growt

67 U2 small nuclear ribonucleoprotein particle (snRNP) are also subunits of the Spt-Ada-Gcn5 acetyltrans

68 U2 small nuclear ribonucleoprotein particle (snRNP) associates with U4/U6.U5 tri-snRNP through the U2

69 U2 small nuclear ribonucleoprotein particle (snRNP) complex, which assembles across the intron at the

70 U1 small nuclear ribonucleoprotein particle (snRNP).

71 and U2 small nuclear ribonucleic particles (snRNPs) and suggested that RBM20-dependent splicing repr

72 in the form of ribonucleoprotein particles (snRNPs) that are comprised of the U1 snRNA and 10 core c

73 2 small nuclear ribonucleoprotein particles (snRNPs), and transforms into a catalytically active spli

74 r small nuclear ribonucleoprotein particles (snRNPs).

75 Gemin5 in small nuclear ribonucleic protein (snRNP) biogenesis as well as, potentially, other cellula

76 These SMN protein heteromers restore snRNP assembly of Sm proteins onto snRNA and completely

77 protein coilin is responsible for retaining snRNPs, the tether for scaRNPs is not known.

78 izes the U1 small nuclear ribonucleoprotein (snRNP) and is essential for the localization of reporter

79 urvival and small nuclear ribonucleoprotein (snRNP) assembly, demonstrating intragenic complementatio

80 to loss of small nuclear ribonucleoprotein (snRNP) assembly.

81 system for small nuclear ribonucleoprotein (snRNP) assembly.

82 wed that U1 small nuclear ribonucleoprotein (snRNP) associates with RNAP II, and both RNAP II and U1

83 ccurs by U1 small nuclear ribonucleoprotein (snRNP) binding the 5' SS and recognition of the BS by th

84 U6 small nuclear ribonucleoprotein (snRNP) biogenesis is essential for spliceosome assembly,

85 anism of U6 small nuclear ribonucleoprotein (snRNP) biogenesis is not well understood.

86 and coilin/small nuclear ribonucleoprotein (snRNP) co-localization are significantly impaired in SH-

87 t of the U5 small nuclear ribonucleoprotein (snRNP) complex of the spliceosome.

88 of the 7SK small nuclear ribonucleoprotein (snRNP) complex, is recruited to the promoters of Pol II-

89 to the 7SK small nuclear ribonucleoprotein (snRNP) complex.

90 ibitory 7SK small nuclear ribonucleoprotein (snRNP) complex.

91 s to the U2 small nuclear ribonucleoprotein (snRNP) component SF3B1.

92 oSNP1, a U1 small nuclear ribonucleoprotein (snRNP) component, likely in a manner dependent on direct

93 The U2 small nuclear ribonucleoprotein (snRNP) has an essential role in the selection of the pre

94 with the U2 small nuclear ribonucleoprotein (snRNP) of the spliceosome.

95 The 7SK small nuclear ribonucleoprotein (snRNP) plays a central role in RNA polymerase II elongat

96 The 7SK small nuclear ribonucleoprotein (snRNP) sequesters and inactivates the positive transcrip

97 RNA by a U1 small nuclear ribonucleoprotein (snRNP) splicing factor.

98 in the 7SK small nuclear ribonucleoprotein (snRNP), which contains, additionally, 7SK snRNA, methyl

99 nactive 7SK small nuclear ribonucleoprotein (snRNP).

100 ing with U1 small nuclear ribonucleoprotein (snRNP).

101 omposed of small nuclear ribonucleoproteins (snRNPs) and accessory proteins that excises introns from

102 sisting of small nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins.

103 U1 and U2 small nuclear ribonucleoproteins (snRNPs) bound to the precursor messenger RNA 5' splice s

104 genesis of small nuclear ribonucleoproteins (snRNPs) involved in mRNA splicing.

105 U1 and U2 small nuclear ribonucleoproteins (snRNPs) mark an intron and recruit the U4/U6.U5 tri-snRN

106 assembled small nuclear ribonucleoproteins (snRNPs) or spliceosome.

107 RNA-protein complexes, including ribosomes, snRNPs, snoRNPs, telomerase, microRNAs, and long ncRNAs.

108 ated huge protein) and U7 small nuclear RNP (snRNP) are HLB components that participate in 3' process

109 -splicing, which binds U1 small nuclear RNP (snRNP) through strong base-pairing with U1 snRNA.

110 The 7SK small nuclear RNP (snRNP), composed of the 7SK small nuclear RNA (snRNA), M

111 roteins (RNPs) including small nuclear RNPs (snRNPs).

112 (snRNAs)-and promotes efficient spliceosomal snRNP assembly.

113 es (CBs), major NBs involved in spliceosomal snRNP assembly and their role in genome organization.

114 n orchestrating the assembly of spliceosomal snRNP particles and subsequently regulating the alternat

115 This loss of spliceosomal snRNP production results in increased splicing noise, ev

116 associates with both dSNUP and spliceosomal snRNPs and localizes to nuclear Cajal bodies.

117 In particular, the minor spliceosomal snRNPs are affected, and some U12-dependent introns have

118 though mechanisms of P-TEFb release from the snRNP are becoming clearer, how P-TEFb remains in the 7S

119 nd that msk-null mutants are depleted of the snRNP assembly factor, survival motor neuron, and the Ca

120 ranslated region that are reminiscent of the snRNP code to which Gemin5 binds on snRNAs.

121 y of PPM1G blocks P-TEFb reassembly onto the snRNP to sustain NF-kappaB-mediated Pol II transcription

122 determine the molecular architecture of this snRNP.

123 t the Sm site with its zinc finger and traps snRNP biogenesis intermediates in human and murine motor

124 ulates both the competing U4 release and tri-snRNP discard processes.

125 rough repeated rounds of disassembly and tri-snRNP reassociation.

126 construction of Saccharomyces cerevisiae tri-snRNP at 5.9 A resolution to reveal the essentially comp

127 e either reverses assembly by discarding tri-snRNP or proceeds to activation by irreversible U4 loss.

128 icate telestem dynamics are critical for tri-snRNP assembly and stability.

129 rogated growth in cancer cells with high tri-snRNP levels.

130 3-angstrom core resolution and the human tri-snRNP at 2.9-angstrom resolution.

131 /U6 protein FgPrp31, which may result in tri-snRNP stabilization.

132 re potentially facilitate the docking of tri-snRNP into the spliceosome.

133 nd protrudes over the concave surface of tri-snRNP, where the U1 snRNP may reside before its release

134 Inhibition of PRPF6 and other tri-snRNP complex proteins, but not other snRNP spliceosome

135 4/U6.U5 small nuclear ribonucleoprotein (tri-snRNP).

136 0 can be successfully assembled into the tri-snRNP (albeit at a lower level than the WT Brr2) and the

137 e first time that PRPF6, a member of the tri-snRNP (small ribonucleoprotein) spliceosome complex, dri

138 rtant bridge between U4/U6 and U5 in the tri-snRNP and comparison with a Prp24-U6 snRNA recycling com

139 in FgSad1 increase the stability of the tri-snRNP and/or the affinity of FgSad1 with U2 snRNP and th

140 The tri-snRNP combines with a precursor messenger RNA substrate

141 ast demonstrated reduced assembly of the tri-snRNP complex.

142 aracterized spontaneous mutations in the tri-snRNP-specific protein, FgSad1, which suppressed the gro

143 and U6 annealed to U4 to reassemble the tri-snRNP.

144 pting mutations in Prp3 lead to U4/U6*U5 tri-snRNP assembly and splicing defects in vivo.

145 ure of Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at 3.7 A resolution led to an essentially complete

146 s in assembly of the megaDalton U4/U6.U5 tri-snRNP complex, and lead to a dynamic model for U4/U6 pai

147 es, probably by stabilizing the U4/U6.U5 tri-snRNP complex.

148 ron microscopy structure of the U4/U6.U5 tri-snRNP illustrates how proteins scaffold the RNA and dram

149 U4/U6.U5 tri-snRNP is a 1.5-megadalton pre-assembled spliceosomal com

150 U4/U6.U5 tri-snRNP represents a substantial part of the spliceosome b

151 article (snRNP) associates with U4/U6.U5 tri-snRNP through the U2/U6 helix II and an interface betwee

152 t sequence, associates with the U4/U6.U5 tri-snRNP to form the fully assembled precatalytic pre-B spl

153 n eukaryotes and is part of the U4/U6.U5 tri-snRNP, a large ribonucleoprotein complex that comprises

154 Following binding of the U4/U6.U5 tri-snRNP, the spliceosome either reverses assembly by disca

155 ractions with components of the U4/U6.U5 tri-snRNP.

156 mark an intron and recruit the U4/U6.U5 tri-snRNP.

157 for a detailed molecular dissection of the U snRNP assembly reaction.

158 The assembly of spliceosomal U snRNPs depends on the coordinated action of PRMT5 and SM

159 U1 snRNP (U1), vertebrates' most abundant non-coding (splic

160 U1 snRNP binds to the 5' exon-intron junction of pre-mRNA a

161 U1 snRNP inserts the 5'SS-U1 snRNA helix between the two Re

162 U1 snRNP plays a critical role in 5'-splice site recognitio

163 t this interaction between the galectin-3-U1 snRNP particle and the pre-mRNA results in a productive

164 ct can be reconstituted by the galectin-3-U1 snRNP particle, isolated by immunoprecipitation of the 1

165 hese results indicate that the galectin-3-U1 snRNP-pre-mRNA ternary complex is a functional E complex

166 Using a U1 snRNP complementation assay, we found that SL4 is essent

167 0S particle that contained galectin-3 and U1 snRNP and this particle was sufficient to load the galec

168 ciates with RNAP II, and both RNAP II and U1 snRNP are also the most abundant factors associated with

169 flanking introns allowed normal U2AF and U1 snRNP binding to the target exon splice sites but blocke

170 cal changes in the levels of U1 snRNA and U1 snRNP proteins.

171 iating an interaction between RNAP II and U1 snRNP.

172 f the human pre-B complex captured before U1 snRNP dissociation at 3.3-angstrom core resolution and t

173 mpletely disrupts the association between U1 snRNP and both FUS and RNAP II, but has no effect on the

174 lyzed here are unable to efficiently bind U1 snRNP proteins.

175 election of 5'-splice site nucleotides by U1 snRNP is achieved predominantly through basepairing with

176 splice site of pre-mRNA is recognised by U1 snRNP.

177 how the ribonucleoprotein particle called U1 snRNP engages with 5' splice sites.

178 recruitment of the spliceosome component U1 snRNP to cognate intronic positions.

179 varying degrees and associated with core U1 snRNP proteins to a lesser extent than the canonical U1

180 splicing factor implicated in displacing U1 snRNP from the 5' splice site.

181 g an essential canonical splicing factor (U1 snRNP) to this pathway provides strong new evidence that

182 Our findings underscore a wider role for U1 snRNP in splicing regulation and reveal a novel approach

183 factors transiently associate with human U1 snRNP and are not amenable for structural studies, while

184 sociated auxiliary proteins recruit human U1 snRNP in alternative splicing.

185 essential subunits of the yeast and human U1 snRNP, respectively, that are implicated in the establis

186 gion architecturally similar to the human U1 snRNP.

187 low-resolution crystal structure of human U1 snRNP.

188 Our observations linking U1 snRNP to ALS patient cells with FUS mutations, SMN-conta

189 roteins and U1 snRNA), but not the mature U1 snRNP-specific proteins (U1-70K, U1A and U1C), co-misloc

190 Moreover, U1 snRNP interacts with transcriptionally engaged RNA polym

191 ggests that a sizable fraction of nuclear U1 snRNP is associated with Gro.

192 ve uncovered a previously unknown role of U1 snRNP beyond the processing of precursor mRNA, and provi

193 tively longer lengths and lower ratios of U1 snRNP binding to intronic polyadenylation sites.

194 ultimately influences the utilization of U1 snRNP in splicing.

195 Significantly, knockdown of U1 snRNP in zebrafish results in motor axon truncations, a

196 of splicing proteins, and in the case of U1 snRNP we saw reciprocal changes in the levels of U1 snRN

197 Nuclear extracts were depleted of U1 snRNP with a concomitant loss of splicing activity.

198 down of Gro or snRNP-U1-C (a component of U1 snRNP) showed a significant overlap between genes regula

199 (yeast) homologs are stable components of U1 snRNP.

200 in the majority of human genes depends on U1 snRNP (U1) to co-transcriptionally suppress transcriptio

201 of these transcripts, suggesting that PSI-U1 snRNP interactions coordinate the behavioral network und

202 mplex U1 small nuclear ribonucleoprotein (U1 snRNP).

203 ng productive docking of the spliceosomal U1 snRNP to a suboptimal 5' splice site.

204 These results show that U1 snRNP acts widely to tether and mobilize lncRNAs to chro

205 Conversely, we found that U1 snRNP does not interact with RNAP II in FUS knockdown ex

206 ing Gems, and motor neurons indicate that U1 snRNP is a component of a molecular pathway associated w

207 omplex is a functional E complex and that U1 snRNP is required to assemble galectin-3 onto an active

208 We previously found that U1 snRNP is the most abundant FUS interactor.

209 polyadenylation site, independent of the U1 snRNP (U1 small nuclear ribonucleoprotein).

210 Here, we report that components of the U1 snRNP core particle (Sm proteins and U1 snRNA), but not

211 e concave surface of tri-snRNP, where the U1 snRNP may reside before its release from the pre-mRNA 5'

212 Acute depletion of U1 snRNA or of the U1 snRNP protein component SNRNP70 markedly reduces the chr

213 interaction between the pre-mRNA and the U1 snRNP, in which a short RNA duplex is established betwee

214 U1A, a component of the U1 snRNP, is known to inhibit polyadenylation upon direct b

215 PSI mutants lacking the U1 snRNP-interacting domain (PSIDeltaAB mutant) exhibit ext

216 bserved with FUS, knockdown of any of the U1 snRNP-specific proteins results in a dramatic loss of SM

217 RNA-mediated mode of interaction with the U1 snRNP.

218 These U1 snRNP sites and PAS sites are progressively gained and l

219 -like variants to form snRNPs and bind to U1 snRNP proteins.

220 Thus, our results demonstrate unique U1 snRNP pathology and implicate abnormal RNA splicing in A

221 alectin-3 molecules not in a complex with U1 snRNP (fraction 1 of the same gradient), failed to resto

222 otein and RNA-protein interactions within U1 snRNP, and show how the 5' splice site of pre-mRNA is re

223 rroring yeast Prp42/Prp39, supports yeast U1 snRNP as a model for understanding how transiently assoc

224 , we report the cryoEM structure of yeast U1 snRNP at 3.6 A resolution with atomic models for ten cor

225 The foot-shaped yeast U1 snRNP contains a core in the "ball-and-toes" region arch

226 ron microscopy structure of the human 17S U2 snRNP at a core resolution of 4.1 angstrom and combine i

227 hat STRAP involves in the assembly of 17S U2 snRNP proteins.

228 coordinated conformational switches among U2 snRNP components.

229 SF3B1 stabilizes the interaction between U2 snRNP and branch point (BP) on the pre-mRNA.

230 the target exon splice sites but blocked U2 snRNP assembly in HeLa nuclear extract.

231 the absence of ATP, when complex E forms, U2 snRNP association is unrestricted.

232 are especially dependent on a functional U2 snRNP (small nuclear RNA [snRNA] plus associated protein

233 1, and maintains its open conformation in U2 snRNP, and that U2 snRNA forms a BSL that is sandwiched

234 Pcbp1, and RBM39 stabilizes or increases U2 snRNP recruitment, enhances spliceosome A complex format

235 n complex with a tightly bound U1 but not U2 snRNP.

236 ior data showing that loss of function of U2 snRNP components can interfere with cell growth and indu

237 udemycin E interferes with the ability of U2 snRNP to maintain an H3K36me3 modification in actively t

238 nRNP, as H2A.Z loss results in persistent U2 snRNP association and decreased recruitment of downstrea

239 acts with U2AF65 and SF3b155 and promotes U2 snRNP recruitment to the branch point.

240 f the U2 small nuclear ribonucleoprotein (U2 snRNP).

241 rlying interactions mediating the Tat-SF1-U2 snRNP association remain unknown.

242 a new molecular interface of the Tat-SF1-U2 snRNP complex for gene regulation.

243 g six proteins that are components of the U2 snRNP and required for A complex formation.

244 in facilitating interactions between the U2 snRNP complex and ATP-dependent helicases, we examined c

245 nked to interactions of alphaCPs with the U2 snRNP complex and may be mediated by cooperative interac

246 Mutations in the U2 snRNP component SF3B1 are prominent in myelodysplastic s

247 histone methyltransferase that primes the U2 snRNP for interaction with SMN.

248 The 3' region of the U2 snRNP is flexibly attached to the SF3b-containing domain

249 interface between U4/U6 di-snRNP and the U2 snRNP SF3b-containing domain, which also transiently con

250 SF3b1 plays a key role in recruiting the U2 snRNP to the BS.

251 s essential for stable association of the U2 snRNP with the intron branch site (BS) sequence during s

252 n, the proteins U2AF35 and U2AF65 and the U2 snRNP, are able to recognize alternative candidate sites

253 spliceosomal rearrangements involving the U2 snRNP, as H2A.Z loss results in persistent U2 snRNP asso

254 The U2 snRNP-intron interaction is disrupted in all complexes b

255 ins and the pre-mRNA, indicating that the U2 snRNP-intron interaction is Prp43's major target.

256 itment of SR proteins, and binding of the U2 snRNP.

257 4 interacts with the SF3A1 protein of the U2 snRNP.

258 1 as a Tat-SF1-interacting subunit of the U2 snRNP.

259 reased the association of FgSad1 with the U2 snRNP.

260 PRMT9-binding partners, linking PRMT9 to U2 snRNP maturation.

261 -snRNP and/or the affinity of FgSad1 with U2 snRNP and therefore potentially facilitate the docking o

262 shows extensive genetic interactions with U2 snRNP-associated proteins, and RNA sequencing (RNA-seq)

263 ylation strengthens the interaction among U2 snRNPs and affects global pre-mRNA splicing pattern and

264 e expression of core components of U1 and U2 snRNPs, splicing regulators and other post-transcription

265 forms with stoichiometric association of U2 snRNPs and the U2 snRNA is base-paired to the pre-mRNA.

266 PHF5A, a component of U2 snRNPs, can be acetylated at lysine 29 in response to mu

267 hromatin immunoprecipitations showed that U2 snRNPs physically interact with nucleosomes.

268 Sudemycin E induces a dissociation of the U2 snRNPs and decreases their interaction with nucleosomes.

269 currently by two molecules of U2AF or two U2 snRNPs, so none of the components are restricted.

270 We show that PRP31, a component of U4 snRNP, is modified with K63-linked ubiquitin chains by t

271 promotes the assembly of a key module of U5 snRNP while assuring the quality control of PRPF8.

272 entosa assemble less efficiently with the U5 snRNP and bind more strongly to R2TP, with one mutant re

273 PRP31 regulates its interaction with the U5 snRNP component PRP8, which is required for the efficien

274 th RNA splicing regulators, including the U5 snRNP components of the spliceosome, such as EFTUD2.

275 pe is caused by haploinsufficiency of the U5 snRNP gene EFTUD2/SNU114.

276 actor PRPF8 is a crucial component of the U5 snRNP, and together with EFTUD2 and SNRNP200, it forms a

277 ediated through its interactions with the U5 snRNP.

278 ze them, and promote the formation of the U5 snRNP.

279 We propose a model for U6 snRNP assembly that explains how evolutionarily divergen

280 6 snRNP in vitro, and propose a model for U6 snRNP assembly.

281 f RNA structure that is chaperoned by the U6 snRNP protein Prp24.

282 ively, these data identify domains of the U6 snRNP that are critical for one of the first steps in as

283 exes (designated B(028)) revealed that U4/U6 snRNP proteins are released during activation before the

284 te post-transcriptional assembly of yeast U6 snRNP in vitro, and propose a model for U6 snRNP assembl

285 he post-transcriptional assembly of yeast U6 snRNP in vitro, which occurs through a complex series of

286 seems to be critical for retaining U5 and U6 snRNPs during/after spliceosomal activation through its

287 Thus, the HLB concentrates FLASH and U7 snRNP, promoting efficient histone mRNA biosynthesis and

288 ne pre-mRNAs are cleaved at the 3' end by U7 snRNP consisting of two core components: a ~60-nucleotid

289 at the 3' end by a complex that contains U7 snRNP, the FLICE-associated huge protein (FLASH) and his

290 Here, we assembled core U7 snRNP bound to FLASH from recombinant components and ana

291 n and functional properties as endogenous U7 snRNP, and accurately cleaves histone pre-mRNAs in a rec

292 a spliceosomal Sm site but the engineered U7 snRNP is functionally impaired.

293 We demonstrate that semi-recombinant holo U7 snRNP reconstituted in this manner has the same composit

294 actors, forming catalytically active holo U7 snRNP.

295 hat distinct domains of FLASH involved in U7 snRNP binding, histone pre-mRNA cleavage, and HLB locali

296 The active form of U7 snRNP contains the HLB component FLASH (FLICE-associated

297 that failure to concentrate FLASH and/or U7 snRNP in the HLB impairs histone pre-mRNA processing.

298 LASH interacts with the N terminus of the U7 snRNP protein Lsm11, and together they recruit the HCC.

299 re-mRNA 3'-end processing by facilitating U7-snRNP recruitment through physical interaction with the

300 ent through physical interaction with the U7-snRNP-specific component Lsm11.