ライフサイエンスコーパス: snoRNP

1 fied but a homolog for the eukaryotic 15.5kD snoRNP protein has not been described.

2 nactive conformations and may regulate H/ACA snoRNP activity.

3 teins are involved in the formation of H/ACA snoRNP and telomerase complexes, both involved in essent

4 d in a cotranscriptional manner during H/ACA snoRNP assembly, possibly by binding to the nascent H/AC

5 d in the early biogenesis steps of box H/ACA snoRNP assembly.

6 ain is essential for Shq1p function in H/ACA snoRNP biogenesis in vivo, possibly in an Hsp90-independ

7 er, we show that NOP10, a component of H/ACA snoRNP complexes including telomerase is mutated in a la

8 olved in the early biogenesis steps of H/ACA snoRNP complexes, and Shq1p depletion leads to a specifi

9 Each H/ACA snoRNP consist of four conserved proteins, Cbf5 (the Psi

10 he former is associated with all yeast H/ACA snoRNP core proteins, unlike TLC1 RNA, the endogenous RN

11 ortance of the association of hTR with H/ACA snoRNP core proteins, we have attempted to express hTR i

12 he TbMTr1 complex specializes the SLA1 H/ACA snoRNP for efficient processing of multiple modification

13 uman hUTP23 directly interact with the H/ACA snoRNP protein yNhp2/hNHP2, the RNA helicase yRok1/hROK1

14 We show that the presence of the H/ACA snoRNP proteins Cbf5p, Nhp2p and Nop10p, but not Gar1p,

15 eins Spt16p, Tfg1p, and Sub1p and with H/ACA snoRNP proteins.

16 ssociation with three of the four core H/ACA snoRNP proteins.

17 he cellular level of pseudouridine, an H/ACA snoRNP-mediated modification of rRNA and other RNAs that

18 hich is likely to be common to all box H/ACA snoRNP-substrate complexes.

19 ity for Shq1p and a direct link to the H/ACA snoRNP.

20 p is the Psi synthase component of box H/ACA snoRNPs and suggest that the pseudouridylation of rRNA,

21 Nonetheless, functional H/ACA snoRNPs assembled in cytosolic extracts are stable and d

22 ryo-EM structures of endogenous insect H/ACA snoRNPs containing two protomers assembled on a two-hair

23 abilizes the association of Nhp2p with H/ACA snoRNPs expressed in vivo.

24 Ps or mature Cbf5- and Gar1-containing H/ACA snoRNPs from tgs1Delta cells.

25 g protein Naf1p, which is required for H/ACA snoRNPs stability, associates with RNA polymerase II-ass

26 CA small nucleolar ribonucleoproteins (H/ACA snoRNPs) facilitate essential cellular processes such as

27 ng the four conserved core proteins of H/ACA snoRNPs, a kinetoplastid-specific protein designated met

28 associated with two core components of H/ACA snoRNPs, hGar1p and Dyskerin (the human counterpart of y

29 Among the known protein components of H/ACA snoRNPs, the essential nucleolar protein Cbf5p is the mo

30 haracterized NAP57 is specific for box H/ACA snoRNPs, whereas the newly identified NAP65, the rat hom

31 function of RNA modification-competent H/ACA snoRNPs, which play pivotal roles in cellular processes

32 skerin is the catalytic subunit of box H/ACA snoRNPs.

33 y are not strongly associated with box H/ACA snoRNPs.

34 Cbf5p, two core proteins of mature box H/ACA snoRNPs.

35 and required for the stability of box H/ACA snoRNPs.

36 that yeast telomerase is unrelated to H/ACA snoRNPs.

37 f TMG caps, nor was the composition of H/ACA snoRNPs.

38 nd is probably not a common component of all snoRNPs.

39 Although snoRNP composition has been thoroughly characterized, th

40 report of a protein component specific to an snoRNP essential for processing of the large ribosomal s

41 er, RNA degradation, snRNA modification, and snoRNP biogenesis).

42 well-characterized murine model of the anti-snoRNP autoimmune response, for the ability to selective

43 immunogenic/antigenic material for the anti-snoRNP response in scleroderma.

44 NOP58 sumoylation and accelerate the C/D box snoRNP assembly.

45 s common protein subunits with the H/ACA box snoRNPs.

46 divergent box C'/D' motifs that are bound by snoRNP proteins.

47 -negative mutant of SMN (SMNDeltaN27) causes snoRNPs to accumulate outside of the nucleolus in struct

48 a small nucleolar ribonucleoprotein complex (snoRNP).

49 is of small nucleolar RNA-protein complexes (snoRNPs) consists of synthesis of the snoRNA and protein

50 arge population of snoRNA-protein complexes (snoRNPs), which create modified nucleotides and particip

51 small nucleolar ribonucleoprotein complexes (snoRNPs) via the targeting of Nhp2 and Nop58.

52 thesis of the snoRNA and protein components, snoRNP assembly, and localization to the nucleolus.

53 this study identifies a lncRNA that connects snoRNP-guided rRNA 2'-O-methylation to upregulated prote

54 o impairs localization of C/D and H/ACA core snoRNP proteins Nop1p and Gar1p, suggesting a defect(s)

55 teractions with box C/D snoRNAs and the core snoRNP protein, Snu13.

56 d TIP49 make multiple interactions with core snoRNP proteins and biogenesis factors and that these in

57 n sequence to Nop58p, is a bona fide box C+D snoRNP component; all tested box C+D snoRNAs were coprec

58 In the recently observed U3 box C/D snoRNP as part of the 90 S small subunit processome, the

59 P complexes, but their exact role in box C/D snoRNP biogenesis is largely uncharacterized.

60 factors are directly involved in U8 box C/D snoRNP biogenesis.

61 ins are restructured during human U3 box C/D snoRNP biogenesis; however, the molecular basis of this

62 g defects and the stable assembly of box C/D snoRNP complexes, suggesting that NOP2/NSUN1-mediated de

63 ific protein component of functional box C/D snoRNP complexes.

64 Archaeal homologs of the box C/D snoRNP core proteins fibrillarin and Nop56/58 have also

65 and support an asymmetric model for box C/D snoRNP organization.

66 stem to demonstrate that assembly of box C/D snoRNP proteins is the step affected by snoRNA location,

67 ey functional nucleoproteins such as box C/D snoRNP.

68 Furthermore, numerous box C/D snoRNPs accumulate on pre-ribosomes in the absence of Db

69 an early regulator for biogenesis of box C/D snoRNPs and controls steady-state levels of box C/D snoR

70 Our data argue that box C/D snoRNPs are asymmetric, with the C' box contacting Nop56

71 small nucleolar ribonucleoproteins (box C/D snoRNPs in eukaryotes), respectively.

72 associated with mature Nop58-containing C/D snoRNPs or mature Cbf5- and Gar1-containing H/ACA snoRNP

73 lar size and promote localization of box C/D snoRNPs to nucleoli, suggesting a role in rRNA maturatio

74 e protein composition and association of C/D snoRNPs with the small subunit (SSU) processosome were n

75 sing (RNase P), RNA modification (H/ACA, C/D snoRNPs), and translation (ribosomes), especially by emp

76 Box C/D snoRNPs, factors essential for ribosome biogenesis, are

77 The assembly and maturation of box C/D snoRNPs, factors essential for ribosome biogenesis, occu

78 rk for understanding the assembly of box C/D snoRNPs.

79 r coupling also occurs in eukaryotic box C/D snoRNPs.

80 t are essential for the formation of box C/D snoRNPs.

81 gesting that this may occur with all box C/D snoRNPs.

82 man disorder is the consequence of defective snoRNP pseudouridylation and ribosomal dysfunction.

83 propose that each snoRNA forms two different snoRNPs, subtly different in how the proteins are bound

84 In contrast to the eukaryotic snoRNP complex, where the core proteins are distributed

85 nsight into the highly homologous eukaryotic snoRNPs.

86 nts of the U3 snoRNP, representing the first snoRNP components identified by using the two-hybrid met

87 and Bcd1-Snu13 interactions are critical for snoRNP assembly and ribosome biogenesis.

88 n, and that active splicing is essential for snoRNP assembly.

89 Among the factors required for snoRNP biogenesis in yeast is Shq1p, an essential protei

90 it to act as a molecular adaptor for guiding snoRNP assembly in similar fashion in all archaea and eu

91 However, snoRNP biogenesis in vivo requires multiple factors to c

92 s Nop1p and Gar1p, suggesting a defect(s) in snoRNP assembly or trafficking to the nucleolus.

93 ting that they function at an early stage in snoRNP biogenesis.

94 expression causes a substantial increase in snoRNPs associated with 60S-90S preribosomal RNP complex

95 In contrast, splicing-independent snoRNP assembly can occur in vitro on snoRNAs that posse

96 ling efficiency of snoRNPs and by inhibiting snoRNP access to proximal target sites.

97 mal particles and their stable assembly into snoRNP complexes.

98 ding yeast) for maturation and assembly into snoRNPs prior to accumulation in their primary functiona

99 e we present a characterization of mammalian snoRNPs.

100 A mature snoRNP has been reconstituted in vitro and is composed o

101 e bridging biogenesis factors, to the mature snoRNP.

102 U14 processing snoRNPs and several modifying snoRNPs examined.

103 The possibility that modifying snoRNPs might affect ribosome structure in other ways is

104 vestigate this process, we have analyzed non-snoRNP factors associated with the nucleoplasmic human U

105 he core box C/D proteins as well as many non-snoRNP factors linked to snoRNP assembly (TIP48, TIP49,

106 Moreover, the Nopp140-snoRNP interaction appears to be conserved in yeast, bec

107 anism for preventing premature activation of snoRNP catalytic activity.

108 ligase TAF15 and NOP58, a core component of snoRNP that guides rRNA methylation, to regulate NOP58 s

109 orated in vivo by the exclusive depletion of snoRNP proteins from nucleoli in cells transfected with

110 nding of the timing and ordered hierarchy of snoRNP action in pre-40S maturation and reveal a novel m

111 ion and reveal a novel mode of regulation of snoRNP function by an RNA helicase in human cells.

112 umor suppressor p53 can act as a sentinel of snoRNP perturbation, the activation of which mediates th

113 cleroderma fibroblasts, suggests a source of snoRNP to initiate and maintain these autoantibody respo

114 ghetti is necessary for the stabilization of snoRNP core proteins and target of rapamycin activity an

115 e that Rvb2 is involved in an early stage of snoRNP biogenesis and may play a role in coupling snoRNA

116 tions that likely control the early steps of snoRNP maturation and contribute to the essential role o

117 ose that Nopp140 functions as a chaperone of snoRNPs in yeast and vertebrate cells.

118 ed on studies in yeast is that each class of snoRNPs is composed of a unique set of proteins.

119 tegral component of the box H + ACA class of snoRNPs, which function to target the enzyme to its site

120 The two major classes of snoRNPs, box H/ACA and box C/D, function in the pseudour

121 otein Nopp140 interacts with both classes of snoRNPs.

122 n leads to dissociation of the components of snoRNPs and the telomerase complex.

123 cription is arrested in nucleoli depleted of snoRNPs, raising the possibility of a feedback mechanism

124 ta provide new insights into the dynamics of snoRNPs on pre-ribosomal complexes and the remodelling e

125 tion by reducing the recycling efficiency of snoRNPs and by inhibiting snoRNP access to proximal targ

126 MN complex in the assembly and metabolism of snoRNPs.

127 ay be involved in the binding and release of snoRNPs from pre-rRNA.

128 specific guide RNA component of the sRNP or snoRNP and the target rRNA.

129 essing snoRNP but is also present with other snoRNPs.

130 small nucleolar ribonucleoprotein particle (snoRNP) with the same four core proteins, NAP57 (also kn

131 small nucleolar ribonucleoprotein particles (snoRNP), a few of which are essential for processing pre

132 Small nucleolar ribonucleoprotein particles (snoRNPs) are essential cofactors in ribosomal RNA metabo

133 small nucleolar ribonucleoprotein particles (snoRNPs) are essential for the maturation and pseudourid

134 small nucleolar ribonucleoprotein particles (snoRNPs) in eukaryotes that are responsible for site spe

135 Small nucleolar ribonucleoprotein particles (snoRNPs) mainly catalyze the modification of rRNA.

136 small nucleolar ribonucleoprotein particles (snoRNPs) that are involved in posttranscriptional proces

137 small nucleolar ribonucleoprotein particles (snoRNPs) that play diverse and essential roles in riboso

138 inct distributions of U8 pre-snoRNAs and pre-snoRNP complexes in HeLa cell nuclear and cytoplasmic ex

139 res: (a) the existence of a protein-only pre-snoRNP complex containing five assembly factors and two

140 /D proteins in a partially reconstituted pre-snoRNP.

141 These proteins are key components of the pre-snoRNP complexes, but their exact role in box C/D snoRNP

142 probably reflects the conversion of the pre-snoRNP, where core protein-protein interactions are main

143 ng that they mediate the assembly of the pre-snoRNP.

144 y to be involved in the formation of the pre-snoRNP.

145 The distinct distribution of U8 pre-snoRNP complexes between the two cellular compartments t

146 vBL AAA(+) adenosine triphosphatase from pre-snoRNPs; and (d) a potential mechanism for preventing pr

147 NA, copurifies with the snR30/U17 processing snoRNP but is also present with other snoRNPs.

148 mplexes, including the U3 and U14 processing snoRNPs and several modifying snoRNPs examined.

149 embly complex coordinates snoRNA processing, snoRNP assembly, restructuring, and localization.

150 such as transcription, DNA damage response, snoRNP assembly, cellular transformation, and cancer met

151 CA small nucleolar (sno) ribonucleoparticle (snoRNP) biogenesis and assembly factor.

152 e box C/D small nucleolar ribonucleoprotein (snoRNP) assembly and ribosomal RNA processing.

153 erase and small nucleolar ribonucleoprotein (snoRNP) complexes.

154 of the U3 small nucleolar ribonucleoprotein (snoRNP) from the yeast Saccharomyces cerevisiae.

155 of the U3 small nucleolar ribonucleoprotein (snoRNP) is central to the events surrounding pre-rRNA pr

156 The U3 small nucleolar ribonucleoprotein (snoRNP) is required for three cleavage events that gener

157 LA1 H/ACA small nucleolar ribonucleoprotein (snoRNP) particle that guides SL psi(28) formation.

158 of H/ACA small nucleolar ribonucleoprotein (snoRNP) particles is mutated in X-linked recessive DC.

159 alyzed by the H/ACA small ribonucleoprotein (snoRNP) complex that shares four core proteins, dyskerin

160 enzymatic small nucleolar ribonucleoprotein, snoRNP) are frequently overexpressed in both murine and

161 ox H/ACA small nucleolar ribonucleoproteins (snoRNPs) and sequences in other eukaryotic RNAs target s

162 Box C/D small nucleolar ribonucleoproteins (snoRNPs) contain four core proteins: fibrillarin, Nop56,

163 Small nucleolar ribonucleoproteins (snoRNPs) guide the folding, modification, and processing

164 box C/D small nucleolar ribonucleoproteins (snoRNPs) involves the sequential recruitment of core pro

165 class of small nucleolar ribonucleoproteins (snoRNPs) is primarily responsible for catalyzing the iso

166 (rRNA), small nucleolar ribonucleoproteins (snoRNPs), and their chaperone, Nopp140 (gene name NOLC1)

167 grity of small nucleolar ribonucleoproteins (snoRNPs).

168 ox H/ACA small nucleolar ribonucleoproteins (snoRNPs).

169 NPs) and small nucleolar ribonucleoproteins (snoRNPs)], which are conserved from archaea to eukaryote

170 otein (U1-snRNP) and U3-small nucleolar RNP (snoRNP) in apoptotic cells.

171 Two core small nucleolar RNP (snoRNP) proteins, Nop1p (fibrillarin in vertebrates) and

172 a autoantigens such as small nucleolar RNPs (snoRNP) also associate with phosphoproteins in response

173 The box C/D small nucleolar RNPs (snoRNPs) are essential for the processing and modificati

174 majority of box H/ACA small nucleolar RNPs (snoRNPs) have been shown to direct site-specific pseudou

175 cleolus and are called small nucleolar RNPs (snoRNPs), while in archaea they are known as small RNPs

176 for association between TbMTr1 and the SLA1 snoRNP but does not affect U1 small nuclear RNA methylat

177 eudouridylase activity is dependent on snR81 snoRNP in vivo.

178 tein complexes, including ribosomes, snRNPs, snoRNPs, telomerase, microRNAs, and long ncRNAs.

179 Our data suggest that retention of such snoRNPs on pre-ribosomes when Dbp3 is lacking may impede

180 ing blockage experiments further reveal that snoRNP proteins bind specifically at the spliceosomal C1

181 We suggest that snoRNP assembly involves an intricate series of interact

182 The snoRNP-associated phosphoprotein complex is composed of

183 NA helicase activity but may be aided by the snoRNP core proteins.

184 de region, function as binding sites for the snoRNP proteins including the enzymatic subunit fibrilla

185 e interaction of TgNoAP1 with factors of the snoRNP and R2TP complexes indicates this protein has a r

186 Our data imply that the snoRNP assembly factor NUFIP can regulate the interactio

187 and/or recruitment of these proteins to the snoRNP complex is induced by multiple apoptotic stimuli

188 The snoRNPs mediate these functions through direct base pair

189 fficient association and dissociation of the snoRNPs, however, how such hierarchy is established has

190 ation and the structural organization of the snoRNPs.

191 fication at adjacent sites suggests that the snoRNPs guiding such modifications likely interact stoch

192 protein Nopp140 (Srp40p) associates with the snoRNPs.

193 Autoantibodies to snoRNP components were more frequent in patients with di

194 as well as many non-snoRNP factors linked to snoRNP assembly (TIP48, TIP49, Nopp140), RNA processing

195 ins why the access of substrate sequences to snoRNPs is facile and how uridine selection may occur wh

196 romyces cerevisiae, depletion of Mpp10, a U3 snoRNP-specific protein, halts 18S rRNA production and i

197 veals that both proteins are critical for U3 snoRNP function in pre-18S rRNA processing at the A0, A1

198 However, as with other U3 snoRNP-specific proteins, neither Imp3p nor Imp4p is req

199 Thus, the role in processing of the U3 snoRNP can be separated into cleavage at the A0 site, wh

200 0p is a specific protein component of the U3 snoRNP in yeast.

201 larin-positive patients suggests that the U3 snoRNP particle is a source of immunogenic/antigenic mat

202 novel protein components specific to the U3 snoRNP with critical roles in pre-rRNA cleavage events.

203 sly undescribed protein components of the U3 snoRNP, representing the first snoRNP components identif

204 The U3 snoRNP, via base pairing, and its associated proteins, a

205 oteins that physically associate with the U3 snoRNP-specific protein, Mpp10p, we have identified Imp3

206 and for antibodies to fibrillarin and the U3 snoRNP-specific proteins Mpp10 and hU3-55K.

207 further elucidate the composition of the U3 snoRNP.

208 duction and impairs cleavage at the three U3 snoRNP-dependent sites: A0, A1, and A2.

209 ibodies to protein components specific to U3 snoRNP, particularly Mpp10.

210 ces on both strands of the analog of the U65 snoRNP pseudouridylation pocket in HJ1 pair with its sub

211 U8 snoRNP is required for accumulation of mature 5.8S and 2

212 ursor complex suggests that the mammalian U8 snoRNP is exported during biogenesis.

213 t of a small nucleolar ribonucleoprotein (U8 snoRNP) required for accumulation of mature 5.8S and 28S

214 We previously demonstrated that U8 snoRNP is essential for processing of both 5.8 and 28 S

215 Here we show that the U8 snoRNP is also restructured, suggesting that this may oc

216 es of U8 RNA show that this region of the U8 snoRNP is necessary for processing of pre-rRNA but not s

217 proteins comprising, or recruited by, the U8 snoRNP modulate the efficiency of cleavage.

218 Analysis of in vivo snoRNP assembly indicated that Nop56p was stably associa

219 ticular, the dense fibrillar component where snoRNPs are localized.

220 n of the Psi in 5.8S rRNA is associated with snoRNP activity, the pseudouridylation of 5S rRNA is not

221 rect evidence linking SUMO modification with snoRNP function.

222 lay a role in coupling snoRNA synthesis with snoRNP assembly and localization.

223 sociation of the phosphoprotein complex with snoRNPs in cells treated with the xenobiotic agent mercu

224 sociation of phosphorylated SR proteins with snoRNPs in cells undergoing apoptosis suggests that the

225 Monoclonal antibodies reactive with snoRNPs precipitated a phosphoprotein complex (pp42, pp3