ライフサイエンスコーパス: spheroid

1 ructures with less physiological relevance ('spheroids').

2 egulates the transport of compounds into the spheroid.

3 by a rapid diffusion into the center of the spheroid.

4 for proliferation and survival in lung tumor spheroids.

5 derm spheroids and stepwise into hepatoblast spheroids.

6 opatterned substrates induces stem-cell-like spheroids.

7 and increased dissociation from organotypic spheroids.

8 wn in 2D culture and further demonstrated in spheroids.

9 of MMAE being similar against monolayers and spheroids.

10 rsed-settled cells are representative of the spheroids.

11 screening of anti-cancer drugs in 3D tumour spheroids.

12 the total contractility of arbitrarily sized spheroids.

13 cells obtained from cultured ovarian cancer spheroids.

14 o-dimensional cultures and three-dimensional spheroids.

15 -10 Gy) and/or heated (0-240 CEM(43)) HCT116 spheroids.

16 ced the size and morphology of cardiomyocyte spheroids.

17 riety of metabolites in different regions of spheroids.

18 I and IV) to drive the formation of the cell spheroids.

19 e penetration in ex vivo tumor multicellular spheroids.

20 ndogenous metabolites in breast cancer MCF-7 spheroids.

21 tides are indispensable for forming the cell spheroids.

22 arried out in a microsystem on multicellular spheroids.

23 he iA process leads to the formation of cell spheroids.

24 rimental measurements of multicellular tumor spheroids.

25 in favor of single MECs migrating away from spheroids.

26 ignore the complex structural properties of spheroids.

27 ass content of individual cell nuclei within spheroids.

28 screens in 2D monolayers and 3D lung-cancer spheroids.

29 ized to examine nanoparticle behavior in the spheroids.

30 on in hypoxic regions of human oligocortical spheroids.

31 s throughout the entire z-axis of individual spheroids.

32 l motility when cultured with fibroblasts in spheroids.

33 r cells as well as in 3D multicellular tumor spheroids.

34 Using co-cultured tumor spheroids (1:1 mixture of metastatic and non-tumorigenic

35 nhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates va

36 d to a hollow spheroidal cage [4] to a solid spheroid [5] but has largely evaded description and inte

37 r the efficient patterning of cardiovascular spheroids after mesoderm formation from hPSCs.

38 e being observed throughout the depth of the spheroids, although emission intensity still drops off t

39 ltiple therapeutic treatments (n = 690 total spheroids analysed).

40 ing and cell-cell communications on cortical spheroid and organoid patterning.

41 wth of neurites from both PC12 multicellular spheroids and chick embryonic dorsal root ganglia bodies

42 matrix remodeling in the case of mesenchymal spheroids and different modes of cell migration.

43 pact (66 Ma) event resulted in deposition of spheroids and melt glass, followed by deposition of diam

44 ived from spheroids are compared to those of spheroids and monolayers in order to find if the dispers

45 y adding microbeads to the surface of tumour spheroids and observing the distribution over time.

46 ured HR(+) breast cancer and prostate cancer spheroids and patient-derived organoids in 3D extracellu

47 Adult-onset leukoencephalopathy with spheroids and pigmented glia (ALSP) is an autosomal domi

48 d to droplets of phloem sap containing amber spheroids and preserving both organic and inorganic resi

49 growth of breast cancer and prostate cancer spheroids and restored lumen filling in the presence of

50 treatment enhanced growth of Rgnef-knockout spheroids and Rgnef re-expression facilitated NF-kappaB-

51 tiated in 3 dimensions into hepatic endoderm spheroids and stepwise into hepatoblast spheroids.

52 estinal epithelial phenotype occur faster in spheroids and that they are viable for a longer period o

53 ells in the peritoneal cavity as nonadherent spheroids and their adherence to the mesothelium of dist

54 tudy is to investigate the impacts of neural spheroids and vascular spheroids interactions on the reg

55 anisms by which injury leads to formation of spheroids and whether these spheroids have a functional

56 ements of mechanical forces in multicellular spheroids and zebrafish embryonic tissues.

57 riations in cell volume within multicellular spheroids and, further, describe how the process depends

58 were enriched in ALDH(+) cells, formed more spheroids, and expressed increased levels of stemness-re

59 ssemination, induced cortical actin belts in spheroids, and slowed retrograde actin flow.

60 of the dispersed-settled cells derived from spheroids are compared to those of spheroids and monolay

61 diversify when clonal three-dimensional (3D) spheroids are cultured in basement membrane, and one suc

62 CRISPR screens revealed that spheroids are differentially dependent on the mammalian

63 develop a microfluidic model in which tumor spheroids are embedded within 3D collagen matrices with

64 interactions and cell growth kinetics in the spheroids are similar to the early stage of a nonvascula

65 the production of a dynamic system in which spheroids are subjected to in vivo like fluid flow and s

66 However, spheroids are typically handled in well-plates in which

67 Our results suggest that HMT-3522 S1 spheroids are useful as an in vitro model system to stud

68 Hepatocyte spheroids are useful models for mimicking liver phenotyp

69 Tumour spheroids are widely used as an in vitro assay for chara

70 llular spheroids (hereinafter referred to as spheroids) are 3D biological models.

71 Our approach utilizes cultured multicellular spheroids as a 3D cell model and cultured cell monolayer

72 models of microbead infiltration into tumour spheroids as they rely on resolving the trajectories of

73 The proposed spheroid-based approach for validation of CRRC is applic

74 causes proliferation to be localised at the spheroid boundary.

75 , we were able to invert the polarity of the spheroids by culturing them around Matrigel beads allowi

76 is of tissue sections from 3D cell cultures (spheroids) by matrix-assisted laser desorption/ionizatio

77 lture systems, uncovering a vulnerability of spheroid cancer cells deprived of extracellular matrix t

78 ommunities to produce discrete multicellular spheroids capable of both aerobic (oxygen producing) and

79 e present a series of studies utilizing lung spheroid cell-secretome (LSC-Sec) and exosomes (LSC-Exo)

80 As the spheroid grows, cells at the spheroid centre may become hypoxic and die, forming a ne

81 This bacteria-spheroid co-culture model enables mechanistic investigat

82 Here, we present a bacteria-in-spheroid coculture (BSCC) platform that simultaneously t

83 uantifying the contractile forces that tumor spheroids collectively exert on highly nonlinear three-d

84 ucted by Dorie et al. could be used to infer spheroid composition and parameters associated with tumo

85 The spheroids consist of three cell types (cancer, fibroblas

86 Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF

87 erive a scale-invariant relationship between spheroid contractility and the surrounding matrix deform

88 ls exposed to tumor-like metabolic stress in spheroid culture activated the mevalonate pathway to pro

89 bottlenecks to the automation of hepatocyte spheroid culture by tethering 3D hepatocyte spheroids di

90 PrSPCs were enriched by spheroid culture from normal human primary or immortaliz

91 These results illustrate the value of spheroid culture in revealing environmental or spatial d

92 ese models is enhanced by high throughput 3D spheroid culture technologies allowing researchers to ef

93 d apoptosis in p53-deficient cancer cells in spheroid culture.

94 relatively small (~100) number of hepatocyte spheroids cultured in a microfluidic device.

95 3D spheroid cultures allow for the production of multicellu

96 form which allows the facile manipulation of spheroid cultures on a large scale severely limits their

97 The hypoxic environment in the spheroid cultures recruited more T cells compared with d

98 (PDLF) and the gingiva (GF) in monolayer and spheroid cultures were exposed to hypoxia or l-mimosine.

99 In PDLF and GF spheroid cultures, the impact of hypoxia and l-mimosine

100 g cholangiocyte culture and 3D cholangiocyte spheroid cultures, we found that biliatresone and decrea

101 rcinoma, EGFR-TKIs show enhanced efficacy in spheroid cultures.

102 ynergized with EGFR-TKI treatment only in 3D spheroid cultures.

103 accumulation and ROS formation at 40-60 mum spheroid depths were found to be the key factors for the

104 Spheroids derived from DCLK1-overexpressing hepatoma cel

105 We used 3-D cortical spheroids derived from human induced pluripotent stem ce

106 nal brain-like tissue patterning in cortical spheroids derived from human iPSCs.

107 rs, 033-F had notably weaker potency against spheroids despite potency levels of MMAE being similar a

108 spheroid culture by tethering 3D hepatocyte spheroids directly onto surface-modified polystyrene (PS

109 Multicellular 3D spheroids display reproducible BBB features and function

110 f the clinically tested drug perifosine into spheroids during a 24 h period, revealing the fraction o

111 usable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).

112 ors in mice, despite overexpression of ZIP4; spheroids established from these cells had increased sen

113 ow that three-dimensional (3D) multicellular spheroids established with malignant glioma cells, unlik

114 Compared to 2D culture, 3D cardiovascular spheroids exhibited higher levels of sarcomeric striatio

115 iodistribution data, tumor histology images, spheroid experiments, in vivo single-cell measurements,

116 ng liposomes to the outer cell layers of the spheroids, followed by doxorubicin release into the deep

117 ration associated with injury is preceded by spheroid formation along axons.

118 r expressing WLD(s) suppresses Rho-dependent spheroid formation and degeneration in response to injur

119 ule BDP9066 decreased cell proliferation and spheroid formation and induced apoptosis in HGSOC cells,

120 22R-expressing PDAC cells with IL22 promoted spheroid formation and invasive activity, resulting in i

121 dings place Rho-actin and NAD(+) upstream of spheroid formation and may suggest that other mediators

122 ls that survived exhibited stemness markers, spheroid formation and tumorigenesis in Balb/c nude mice

123 Knockdown of NRF2 inhibited spheroid formation by driving PrSPC differentiation, whe

124 d the ability of PTEN variants to rescue the spheroid formation phenotype of PTEN(-/-) MCF10A cells m

125 ; we evaluated cell migration, invasion, and spheroid formation under hypoxic and normoxic conditions

126 improved sensitivity to platinum, decreased spheroid formation, and delayed tumor initiation.

127 n and underlying mechanisms governing axonal spheroid formation, however, remain unclear.

128 w that ACTL6A drives SCC cell proliferation, spheroid formation, invasion and migration, and that the

129 of Wallerian degeneration in injury-induced spheroid formation.

130 enfluridol and analyzed in proliferation and spheroid formation.

131 Here, the response of tumour spheroids formed from two established human cancer cell

132 Here, we investigated spheroid-forming cells in a metastatic TNBC model.

133 g of fibronectin, thus enabling formation of spheroids from a monolayer of cells.

134 drop fusion model: the fusion was faster for spheroids from epithelial cells with lower apparent surf

135 h structure and mechanical properties of the spheroids from human somatic cells of different phenotyp

136 the development of more realistic models for spheroid fusion that would further provide a helpful too

137 based biosensor relies on immobilized HEK293 spheroids genetically engineered with powerful red- and

138 gely due to the reproducible manner in which spheroids grow: the diffusion of nutrients and oxygen fr

139 n in both cell culture and three-dimensional spheroids grown from primary tumors.

140 As the spheroid grows, cells at the spheroid centre may become

141 imal growth at <0.5 mm glutamine, with tumor spheroid growth and monolayer migration of 143B ASCT2ko

142 However, tracking of spheroid growth and treatment-induced volume reduction h

143 ts and that depletion of talin1 inhibited 3D spheroid growth in colon cancer cells.

144 proliferation, it significantly inhibited 3D spheroid growth in fibrin gel assays.

145 Moreover, UL33 increased spheroid growth in vitro and accelerated tumor growth in

146 s antagonized TOPflash reporter activity and spheroid growth in vitro and elevated Wnt-inhibitory fac

147 The mathematical model for tumour spheroid growth is parameterised by multicellular tumour

148 naling intermediate SOS1 was required for 3D spheroid growth of EGFR-mutated NSCLC cells.

149 In conclusion, 3D tumour spheroid growth studies reveal differences in response t

150 cell cycle progression, colony formation, 3D spheroid growth, and tumor xenograft growth in mice.

151 Response was analysed in terms of spheroid growth, cell viability and the distribution of

152 gate deoxysphingolipid synthesis and improve spheroid growth, similar to phenotypes observed with the

153 ib blocked PI3K/AKT activation and inhibited spheroid growth, suggesting an essential role for the PI

154 PRK2) and their depletion results in reduced spheroid growth.

155 significantly reduces cell proliferation and spheroid growth.

156 erentiation, whereas its activation enhanced spheroid growth.

157 uces microenvironmental changes that promote spheroid growth.

158 In addition, tri-cultured spheroids had high levels of TBR1 (deep cortical layer V

159 to formation of spheroids and whether these spheroids have a functional role in degeneration remain

160 Tumour spheroids have a microenvironment that more closely rese

161 Multicellular tumor spheroids have been increasingly used by researchers to

162 The cell aggregates-spheroids-have become widely used model objects in the s

163 hereditary diffuse leukoencephalopathy with spheroids (HDLS), an adult-onset progressive neurodegene

164 Multicellular spheroids (hereinafter referred to as spheroids) are 3D

165 dy, we embedded these partially reprogrammed spheroids in collagen-I matrices of varying densities, m

166 Spheroids in individual zones fused and the maintenance

167 r microenvironment (TME) and ascites-derived spheroids in ovarian cancer (OC) facilitate tumor growth

168 efficient histological analysis of up to 96 spheroids in parallel.

169 rexpressing primary human hepatocytes formed spheroids in suspension cultures.

170 tion of CD8(+) T lymphocytes with tumor cell spheroids in vitro induced a similar phenotype, supporti

171 ired proliferation, and an inability to form spheroids in vitro, while in vivo they generated highly

172 h delay in vivo; they also formed smaller 3D spheroids in vitro.

173 ent cellular uptake in monolayers and tumour spheroids, including when assessed in co-cultures of TNB

174 ckout of Adamts9 in IMCD3 cells, followed by spheroid induction, resulted in defective lumen formatio

175 physiologically realistic model using tumor spheroids instead of single cells under perfusion.

176 the impacts of neural spheroids and vascular spheroids interactions on the regional brain-like tissue

177 behind the development of 3D cardiovascular spheroids into either vascular or cardiac cells has not

178 n both Boyden chamber and 3D hyaluronic acid spheroid invasion assays.

179 r cells also inhibited cell migration and 3D spheroid invasion.

180 of maintaining the coherence of chondrocyte spheroids, leading to a larger quantity of CD44 (by immu

181 load from 033-F was reduced in higher volume spheroids, likely contributing to the potency difference

182 Tissue spheroids, made of human adipose-derived stem cells (ADS

183 While the spheroids' magnetization orientation is consistent with

184 Irradiated spheroids maintained a dense structure and exhibited a l

185 models, we developed 3D multicellular tumour spheroids (MCs) as an intermediate step to bridge the ga

186 wth is parameterised by multicellular tumour spheroid (MCTS) data.

187 Multicellular tumor spheroid (MCTS) systems provide an in vitro cell culture

188 odel by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal s

189 sembles biofilm-like structures in the tumor spheroid microenvironment, whereas heat-killed F. nuclea

190 Here we devised a scalable cancer-spheroid model and performed genome-wide CRISPR screens

191 nce to therapeutics can be integrated into a spheroid model and tracked over time by automated imagin

192 he development of a 3-dimensional (3D) tumor spheroid model that can harbor and promote the growth of

193 el in-vitro 3D human adipocyte/pre-adipocyte spheroid model, we investigated whether adipocytes and t

194 Combined three-dimensional tumor spheroid modeling and machine learning classifies PTEN m

195 xperimentally validated in three-dimensional spheroid models and established uncharacterized crosstal

196 Here, utilizing 3D cancer spheroid models coupled with CRISPR-Cas9 screens, we inv

197 h 2D cell culture and 3D multicellular tumor spheroid models of pancreatic cancer.

198 study of large cell collectives, such as the spheroid models prevalent in mechanobiology.

199 dvancement in the application of 3D tethered spheroid models to high throughput drug screening.

200 on-resistant prostate cancer cell (CRPC) and spheroid models.

201 Drug treatment of 3D cancer spheroids more accurately reflects in vivo therapeutic r

202 as well as autophagic response and restored spheroid morphology.

203 reveals intricate 3D organizations of tumor spheroids, mouse brain tissues, and tumor xenografts.

204 patic 3D model with primary human hepatocyte spheroids, nitrite treatment reduced the degree of metab

205 MLNPs showed the highest efficacy against 3D spheroids of TNBC, in addition the MLNPs also induced hi

206 ccurate positioning of a layer of osteogenic spheroids onto a sacrificial alginate support followed b

207 Current brain spheroids or organoids derived from human induced plurip

208 cancer spheroids, revealing advantages of 3D spheroids over 2D monolayers, wherein novel therapeutic

209 of engineered bacteria within multicellular spheroids over a timescale of weeks.

210 creasing cell death was observed in the HT29 spheroids over the five-day period.

211 gulation, and targeting NRF2 and GPX4 killed spheroids overall.

212 rt followed by another layer of chondrogenic spheroids overlaid by the same support.

213 Collectively, this novel spheroid platform will enable high-throughput anti-cance

214 The metabolomic profiles inside spheroids provide crucial information reflecting the mol

215 ure and exhibited a longer growth delay than spheroids receiving hyperthermia or combination treatmen

216 demonstrate that collective forces of tumor spheroids reflect the contractility of individual cells

217 n response to such matrix constraints, these spheroids regained their fibroblastic properties and spr

218 rsion as, in both cases, a subset of mammary spheroids remained insensitive to local matrix stiffness

219 ubicin release into the deeper layers of the spheroids, resulting in a significant reduction in cell

220 Distinctly, MCF7 and T47D spheroids retained ER protein expression in cocultures b

221 duction studies) of the polystyrene tethered spheroids reveal significant improvements over hepatocyt

222 repeats (CRISPR) screening in 3D lung cancer spheroids, revealing advantages of 3D spheroids over 2D

223 es an cellular flow of tumour cells from the spheroid rim towards its core.

224 eration, such as DR6 and SARM1, mediate post-spheroid rupture events that lead to catastrophic axon d

225 d media collected from wild-type axons after spheroid rupture.

226 s degeneration during development via axonal spheroid rupture.

227 followed by IHC analysis on exactly the same spheroid section.

228 Furthermore chondrocyte spheroids, seeded on top of gels that contained either M

229 -135 kills low-passage patient-derived tumor spheroids selectively over surrounding cancer-associated

230 es exhibit liquid-like properties, including spheroid shapes due to surface tension, fluidity due to

231 th when exposed to heat or radiation: heated spheroids shed dead cells within four days of heating an

232 Spheroids showed different patterns of shrinkage and reg

233 Additionally, the core of the larger spheroids showed more resiliency towards microtubule inh

234 mation and cell migration, alterations of 3D-spheroid size and shape as well as retardation of cell c

235 Spheroid size distribution after 15 days was in the diam

236 there is not yet a robust way of controlling spheroid size, homogeneity and integrity during extended

237 ctions at the highest concentrations for all spheroid sizes.

238 s of large asteroid disruptions, that oblate spheroids, some of which have a pronounced equator defin

239 ion of PI3Kbeta reduced PD-RCC-stimulated EC spheroid sprouting.

240 sis activity in two-dimensional cells, tumor spheroids, subcutaneous transplantation mouse models, an

241 est result with human neural progenitor cell spheroids suggests a remarkable reduction in histology p

242 into tumour spheroids via advection from the spheroid surface, by adding microbeads to the surface of

243 'skin', only a few cell layers thick, at the spheroid surface, which correlates with activation of me

244 oids was investigated and confirmed that the spheroid takes up the complex.

245 onding 250-fold proliferative advantage over spheroid technologies.

246 xicant treatment on functional 3D hepatocyte spheroids tethered directly on polystyrene multi-well pl

247 ctive RGD and galactose ligands required for spheroid tethering and formation.

248 -Rho dependent growth of calcium rich axonal spheroids that eventually rupture, releasing material to

249 cess NAD(+)) neurons, are capable of forming spheroids that eventually rupture, releasing their conte

250 iments were then carried out in human cancer spheroids that provide a realistic tumor model for the d

251 ew differentiation method to generate kidney spheroids that structurally recapitulate important featu

252 inetic parameters associated with the tumour spheroids that they infiltrate.

253 elial changes accompany a previously unknown spheroid to lentoid shape transition of the lens as dete

254 ly drive flow through the system and subject spheroids to capillary like flow velocities.

255 e we exploit the spherical symmetry of tumor spheroids to derive a scale-invariant relationship betwe

256 and assemble them with human skeletal muscle spheroids to generate 3D cortico-motor assembloids.

257 ced by UPR induction, and resensitized tumor spheroids to proteasome inhibition both in vitro and in

258 le strategy for performing CRISPR screens in spheroids to reveal cancer vulnerabilities.

259 on of volumetric in-vitro tumour tissue (SAS spheroids) to demonstrate concurrent operation of laser

260 neage cells derived in human oligodendrocyte spheroids transitioned through developmental stages simi

261 a microfluidic device containing an array of spheroid traps, into which multiple pre-grown colorectal

262 ese experiments revealed that, in irradiated spheroids treated with 1(4+), acute and rapid photoinduc

263 aging or optogenetic stimulation of cortical spheroids triggers robust contraction of 3D muscle, and

264 researchers to efficiently culture numerous spheroids under varied experimental conditions.

265 Interestingly, these spheroids undergo a complex maturation process reminisce

266 After the spheroids underwent hypoxia (0.1% O(2); 24 h), the BBB w

267 The spheroids used here were composed of six brain cell type

268 ration of free-floating 3D posterior foregut spheroids using FGF4, Wnt pathway agonist CHIR99021 (CHI

269 inert microspheres to infiltrate into tumour spheroids via advection from the spheroid surface, by ad

270 of 3 muM doxorubicin (DOX) + MB + US reduced spheroid viability to 48 +/- 2%, compared to 75 +/- 5% o

271 mes (DOX-LS) were conjugated to MBs, reduced spheroid viability to 62 +/- 3%, a decrease compared to

272 s fact contributed to the reduction of tumor spheroids viability and size.

273 owered potency towards spheroids with 033-F, spheroid volume was still readily reduced by 033-F in a

274 ity within the deeper, hypoxic layers of the spheroids was also investigated.

275 complex as a probe, its uptake into 280 mum spheroids was investigated and confirmed that the sphero

276 r down-regulated, the biliatresone effect on spheroids was phenocopied, resulting in lumen obstructio

277 on in MCF-7 cells and three-dimensional (3D) spheroids was proven using the Pt-nanoelectrode.

278 It was found that sensitivity to heat in spheroids was significantly less than that seen in monol

279 nto large (>500 mum) multicellular 3D tissue spheroids was studied using a multimodal imaging approac

280 When Gd-MSN was added to the tumor spheroids, we observed efficient uptake and uniform dist

281 reatic spheroids were established; cells and spheroids were analyzed by immunoblots, reverse transcri

282 Single hybrid spheroids were constructed at different iNPC: iEC: MSC r

283 Sox2-expressing skin-derived precursor (SKP) spheroids were cultured from murine back skin.

284 drug on the spatially resolved metabolites, spheroids were cultured using HCT-116 colorectal cancer

285 C and KPC-ZEB1-knockout mice, and pancreatic spheroids were established; cells and spheroids were ana

286 T29 cells were seeded into the device and 3D spheroids were generated and cultured through the perfus

287 The spheroids were generated by co-culturing pancreatic canc

288 h multiple pre-grown colorectal cancer (CRC) spheroids were loaded.

289 Hepatoblast spheroids were reseeded in a high-throughput format and

290 Spheroids were treated with 5-Fluorouracil for five days

291 Following the initial growth phase, spheroids were treated with auristatin as small molecule

292 articles readily entered the interior of the spheroid, whereas the dissolved dye alone did not penetr

293 nsions (3D) into chondrogenic and osteogenic spheroids, which were confirmed by immunostaining and hi

294 ugal neurons project and connect with spinal spheroids, while spinal-derived motor neurons connect wi

295 Despite lowered potency towards spheroids with 033-F, spheroid volume was still readily

296 approach is used to synthesize well-defined spheroids with controlled composition and morphology.

297 pparent surface tension than for mesenchymal spheroids with higher surface tension.

298 ation or sensitivity to hypoxia, can produce spheroids with similar bulk growth dynamics but differin

299 portantly, treatment of polystyrene tethered spheroids with vehicle and paradigm hepatotoxicant (chlo

300 taneously form acini (also known as cysts or spheroids) with a single, fluid-filled central lumen whe