ライフサイエンスコーパス: spindle pole

1 er partners TRF1 (at telomeres) and NuMA (at spindle poles).

2 ssociation of each centrosome with a mitotic spindle pole.

3 rms microtubule (MT) attachments to only one spindle pole.

4 rs of some chromosomes did not extend to the spindle pole.

5 ores are paired and oriented toward the same spindle pole.

6 ity is greater at the cenexin-positive older spindle pole.

7 s did not separate and did not move toward a spindle pole.

8 rosomes in animal cells naturally become two spindle poles.

9 to dynamic microtubules (MTs) from opposite spindle poles.

10 ents to microtubules emanating from opposite spindle poles.

11 chores to bind to microtubules from opposite spindle poles.

12 eater than two centrosomes, generating extra spindle poles.

13 ey can be evenly distributed towards the two spindle poles.

14 crotubules (MTs) emanating from the opposing spindle poles.

15 shing mal-oriented chromosome arms away from spindle poles.

16 inetochore-MT attachments in the vicinity of spindle poles.

17 o then regroup and merge them into two equal spindle poles.

18 restored by inhibition of Aurora A kinase at spindle poles.

19 kinases Plk1 and Aurora A at centrosomes and spindle poles.

20 rallel architecture that concentrates MTs at spindle poles.

21 .571dupA mutation did not show CKAP2L at the spindle poles.

22 spindles by clustering centrosomes into two spindle poles.

23 ween microtubules that emanate from opposite spindle poles.

24 g that MEI-1 and ASPM-1 act independently at spindle poles.

25 ched kinetochores along microtubules towards spindle poles.

26 o interpolar spindle microtubules and to the spindle poles.

27 bules (MTs) and slide them apart, toward the spindle poles.

28 p build this bipolar array by separating the spindle poles.

29 is essential for the organization of mitotic spindle poles.

30 errors due to extra centrosomes and mitotic spindle poles.

31 controlled in part through activities at the spindle poles.

32 otor dynein mediates ER concentration around spindle poles.

33 Sister kinetochores barely move apart toward spindle poles.

34 persion and centriole mis-positioning at the spindle poles.

35 moved transiently both toward and away from spindle poles.

36 chromosomes, even as chromosomes move toward spindle poles.

37 spindle and make transient contact with the spindle poles.

38 tners attached to microtubules from opposite spindle poles.

39 ather than intrinsic differences between the spindle poles.

40 Gravin binding partner polo-like kinase 1 at spindle poles.

41 embles in an equatorial zone between the two spindle poles.

42 uMA could not efficiently concentrate at the spindle poles.

43 um until the chromosomes are embedded in the spindle poles.

44 e spindle and ultimately toward the opposite spindle poles.

45 g to forces that move chromosomes toward the spindle poles.

46 hes the cross-linking kinesin-5 motor Eg5 at spindle poles [3].

47 manates from chromatin and diminishes toward spindle poles [6].

48 to mammals, Wts kinase localizes to mitotic spindle poles, a prominent site of Mud localization.

49 that cytoplasmic dynein is required for the spindle pole accumulation of Astrin, and dynein-mediated

50 ting that AMPK is essential for pMRLC(ser19) spindle pole activity.

51 th inactive Aurora A and is recruited to the spindle pole after Aurora A inhibition.

52 ne-fly spermatocytes and inward movements of spindle poles after laser cuts across Potorous tridactyl

53 ER redistribution to spindle poles also did not require non-claret disjunctio

54 congression by pushing chromosomes away from spindle poles, although knowledge of the molecular mecha

55 nucleation complex (gammaTC) favors the old spindle pole, an asymmetry inherent to the outer plaque

56 which causes chromosomes to move toward the spindle poles (anaphase A).

57 in which chromosomes move toward stationary spindle poles, anaphase B, in which chromosomes move at

58 d the p150(Glued) subunit of dynactin to the spindle pole and cell cortex and of CLIP170 and p150(Glu

59 iously unidentified component of the mitotic spindle pole and the centrosome.

60 uous K-fibers connect every kinetochore to a spindle pole and the force for chromosome movement is pr

61 otubule-associated protein that localizes to spindle poles and aids in microtubule stabilization, but

62 ed activation of Plk1 and Aurora A kinase at spindle poles and an impaired localization of MCAK and H

63 Ncd cross-links parallel microtubules at the spindle poles and antiparallel microtubules within the s

64 gamma-TuRC) microtubule-organizing centre at spindle poles and can alter its structure and function.

65 g animal cap mitoses, Kif2a localizes to the spindle poles and centromeres.

66 Asp-CaM interaction alone leads to unfocused spindle poles and centrosome detachment.

67 interpolar microtubules, which span the two spindle poles and contribute to mitotic pole separation

68 meiotic spindle domain (LISD), permeates the spindle poles and forms dynamic protrusions that extend

69 phosphorylated AMPK localizes to the mitotic spindle poles and increases when cells enter mitosis.

70 otic spindle, especially concentrated at the spindle poles and inner centromeres in metaphase and tra

71 repeat protein localizing to centrioles and spindle poles and is associated with short stature, onyc

72 for balanced distribution of Astrin between spindle poles and kinetochores.

73 localizes to the cell cortex adjacent to the spindle poles and orients the mitotic spindle.

74 TRIM69, which associates with spindle poles and promotes centrosomal clustering, is es

75 apparatus (NuMA) complex from cell cortex to spindle poles and show that actin filaments counteract s

76 Phosphorylated Hsp72 concentrates on spindle poles and sites of MT-kinetochore attachment.

77 hromosomal missegregation, misorientation of spindle poles and the generation of extra centrosomes, w

78 rent models propose that displacement of the spindle poles and/or the activity of kinetochore microtu

79 est can be independent of their kinetochore, spindle pole, and nuclear envelope localization.

80 vity is increased specifically at the oldest spindle pole, and this increase in activity is lost in c

81 tion in Drosophila Shot localizes to mitotic spindle poles, and its knockdown results in an unfocused

82 branous structures, such as DNA repair foci, spindle poles, and RNA granules.

83 spindle pole, indicating the inner core and spindle pole are independent and co-regulated.

84 olonged in the mutants while two acentriolar spindle poles are assembled.

85 Spindle poles are asymmetric in composition.

86 We find spindle poles are largely dispensable, and in fact act a

87 codes a protein localized to centrosomes and spindle poles, as well as to the primary cilium.

88 a microtubules accumulated, extra functional spindle poles assembled, and chromosomes frequently segr

89 c spindle through Rab11-dependent control of spindle pole assembly and function.

90 ule severing and ASPM-1 both promote meiotic spindle pole assembly in C. elegans oocytes, whereas the

91 d biallelic truncating CSPP1 (centrosome and spindle pole associated protein 1) mutations in 19 JBTS-

92 al analysis and showed that the spindle- and spindle pole-associated clathrin pools are membrane-boun

93 e(19) phosphorylated MRLC (pMRLC(ser19)) and spindle pole-associated pMRLC(ser19) are abolished when

94 ther, these data indicate that AMPK mediates spindle pole-associated pMRLC(ser19) to control spindle

95 at the spindle pole is mutually dependent on spindle pole-associated protein Msd1.

96 Our observations reveal that RED is a spindle pole-associated protein that colocalizes with MA

97 ed to the meiotic spindle, especially to the spindle poles at metaphase, while it was concentrated at

98 Their distinct localizations at spindle poles (AtAurora1), spindle microtubules (AtEDE1)

99 erentially adopt shallow beta angles between spindle pole axis and substratum.

100 LARG colocalizes with alpha-tubulin at the spindle poles before localizing to the central spindle.

101 ere we describe how scaffolding the MEN onto spindle pole bodies (SPB-centrosome equivalent) allows t

102 MEN is a signaling cascade that localizes to spindle pole bodies (SPBs) and activates the phosphatase

103 Centrosomes and spindle pole bodies (SPBs) are membraneless organelles w

104 Cdc5 also localizes to the spindle pole bodies (SPBs) from S phase until the end of

105 orA accumulates at nuclear envelope-embedded spindle pole bodies (SPBs) in a way that requires its ol

106 nt activation, S. pombe Dma1 concentrates at spindle pole bodies (SPBs) in an FHA-dependent manner an

107 (MTOCs), known as centrosomes in animals and spindle pole bodies (SPBs) in fungi, are important for t

108 Tts1 promotes insertion of spindle pole bodies (SPBs) in the NE at the onset of mit

109 Centrosomes, or spindle pole bodies (SPBs) in yeast, are vital mechanica

110 Whereas binding of MEN components to spindle pole bodies (SPBs) is necessary for MEN signalin

111 e two nuclei, whereas dynein accumulating at spindle pole bodies (SPBs) may pull MTs nucleated from t

112 enable nucleocytoplasmic transport, and the spindle pole bodies (SPBs) that mediate chromosome segre

113 ation network) and measured their binding to spindle pole bodies (SPBs), the centrosome equivalent of

114 l unexpected structural modifications of the spindle pole bodies (SPBs), the yeast microtubule organi

115 Second, Ipl1 localizes to the spindle pole bodies (SPBs), where it blocks spindle asse

116 At mitosis NIMA-GFP locates to spindle pole bodies (SPBs), which contain Cdk1/cyclin B,

117 reviously unknown functions localised to the Spindle Pole Bodies (SPBs).

118 ganisms such as fungi, centrosomes [known as spindle pole bodies (SPBs)] are essential for cell divis

119 Here we show that yeast spindle pole bodies (SPBs, yeast centrosomes) differenti

120 requires insertion of centrosomes (known as spindle pole bodies [SPBs]) into fenestrated regions of

121 Yeast centrosomes (called spindle pole bodies [SPBs]) remain cohesive for hours du

122 ation is required for proper localization at spindle pole bodies and the cell division site, E3 ligas

123 At this time, centromeres and spindle pole bodies relocate to the bud neck, explaining

124 Schizosaccharomyces pombe harbors MTOCs at spindle pole bodies, transient MTOCs in the division pla

125 tion of Nak1 causes removal of Nak1 from the spindle pole bodies, which may both relieve Nak1 inhibit

126 Most components of the MEN localize to spindle pole bodies.

127 the speed and acceleration of two separating spindle pole bodies.

128 tion because of dynamic microtubule bundles, spindle-pole bodies, the nuclear envelope, and passive c

129 ast, spindle orientation begins with the old spindle pole body (SPB) (from the preceding cell cycle)

130 ges through the nuclear envelope (NE) at the spindle pole body (SPB) and other sites.

131 , while cytoplasmic microtubules vanish, the spindle pole body (SPB) assembles a long and stable mono

132 ecular architecture of the core of the yeast spindle pole body (SPB) by Bayesian integrative structur

133 Mutations in the spindle pole body (SPB) component Cut12 suppress otherwi

134 In this state, Cdc14 targets the spindle pole body (SPB) component Spc110 to counterbalan

135 (Saccharomyces cerevisiae) the multilayered spindle pole body (SPB) is embedded in the nuclear envel

136 The yeast spindle pole body (SPB) is the functional equivalent of

137 isiae, nuclear pore complexes (NPCs) and the spindle pole body (SPB) must assemble into an intact nuc

138 The spindle pole body (SPB) of budding yeast duplicates once

139 The SIN is assembled at the spindle pole body (SPB) on the scaffold proteins Cdc11 a

140 The Saccharomyces cerevisiae spindle pole body (SPB) serves as the sole microtubule-o

141 dc13 to the yeast centrosome equivalent, the spindle pole body (SPB), and disruption of this motif pr

142 ation initiation network (SIN), an essential spindle pole body (SPB)-associated kinase cascade, which

143 ter side of the bud neck and to the daughter spindle pole body (SPB).

144 ions: the nuclear pore complex (NPC) and the spindle pole body (SPB).

145 Cs) such as the animal centrosome and fungal spindle pole body (SPB).

146 anchors the septum initiation network to the spindle pole body (SPB, centrosome equivalent) to contro

147 The spindle position checkpoint (SPOC) is a spindle pole body (SPB, equivalent of mammalian centroso

148 primes the invariant inheritance of the old spindle pole body (SPB, the yeast centrosome) by the bud

149 nters (MTOCs; mammalian centrosome and yeast spindle pole body [SPB]) nucleate more astral microtubul

150 stent with aggregation of centromeres at the spindle pole body and compartmentalization of individual

151 e1, cytoplasmic microtubules detach from the spindle pole body at high rates.

152 to the nuclear envelope near the site of the spindle pole body during interphase.

153 e strengths of microtubule attachment to the spindle pole body during these stages of the cell cycle.

154 calization and roles for Hei10 in centrosome/spindle pole body dynamics and associated nuclear traffi

155 in sealing the nuclear envelope in mammals, spindle pole body dynamics in fission yeast, and surveil

156 evidence that microtubule nucleators at the spindle pole body help coordinate cytokinetic furrow for

157 r many years that centromeres cluster at the spindle pole body in fission yeast.

158 anized in the nuclear envelope, known as the spindle pole body in yeast (analogous to the centrosome

159 by inactivating centromere attachment to the spindle pole body or changing the position of ribosomal

160 netic nodes around the equator 10 min before spindle pole body separation (cell-cycle time, -10 min)

161 Bilobed nuclei appeared predominantly after spindle pole body separation, suggesting that nuclear en

162 e gamma-tubulin complex to both centrosomal (spindle pole body) and non-centrosomal sites.

163 perhaps by altering its localization to the spindle pole body, and, thus, that gamma-tubulin plays a

164 I1, involved in the duplication of the yeast spindle pole body, as a critical regulator of centriole

165 mplicated three of these genes in centrosome/spindle pole body, centromere, and cohesion function.

166 a diffusion based mechanism, centred on the spindle pole body, for the coordination of DNA replicati

167 ovel antiparallel bundle associated with the spindle pole body, named Q-MT bundle.

168 lication of the yeast centrosome (called the spindle pole body, SPB) is thought to occur through a se

169 e gammaTuRC and anchors the gammaTuRC to the spindle pole body.

170 l early mitosis when they co-localize at the spindle pole body.

171 DNA replication but unable to duplicate the spindle pole body.

172 identify a unique link between NuA4 and the spindle pole body.

173 ype region, from its natural location at the spindle-pole body to the immediate vicinity of the nucle

174 stress response; (2) ESCRT factors regulate spindle-pole-body duplication; and (3) a membrane-protei

175 se, FgCdc14-GFP localized to the nucleus and spindle-pole-body.

176 Poleward streams are integrated at spindle poles, but are diverted by any unattached kineto

177 pulsion of type-1-phosphatase (PP1) from the spindle pole by Fin1 (NIMA) kinase ensures switch-like a

178 iple chromosomes associated with one or both spindle poles, causing a significant mitotic delay.

179 n inner core closely aligned with the unique spindle pole (centrocone) holds distant orthologs of the

180 ust interact with microtubules from opposite spindle poles (chromosome bi-orientation) [1, 2].

181 olar spindle could inhibit SAC silencing and spindle pole clustering could promote it-albeit accompan

182 polarity becomes a function of HSET-mediated spindle pole clustering.

183 romosomes act as a physical barrier blocking spindle pole coalescence and bipolarity.

184 y shorten in the pole-to-pole axis such that spindle poles contact the outer kinetochore before the s

185 One spindle pole contains the oldest mitotic centriole, the

186 of Aurora A and Plk1 to the mother (oldest) spindle pole contributes to the fidelity of symmetric ce

187 ant increase of multipolar mitoses and other spindle pole defects.

188 tromere and leads to lagging chromosomes and spindle pole defects.

189 arly spindle assembly models postulated that spindle pole-derived microtubules search the cytoplasmic

190 We show that proximity to spindle poles destabilizes kinetochore-MTs and that stab

191 m in which kinetochores attached to opposite spindle poles differ in their interactions with MTs and

192 The acentrosomal meiotic spindle poles do not have centrioles and are not anchore

193 ch homolog pair moved together with the same spindle pole during anaphase B spindle elongation.

194 is Gravin scaffold accumulates at the mother spindle pole during metaphase.

195 onse to misaligned chromosomes at the oldest spindle pole during metaphase.

196 ng microtubule asters that grow out from the spindle poles during anaphase.

197 the chromosomes, with polar microtubules and spindle poles during metaphase through telophase, and pa

198 ial occurs when chromosomes move to opposite spindle poles during mitosis.

199 k1 protein stability and localization to the spindle poles during mitosis.

200 s to cross-link parallel microtubules at the spindle poles during mitosis.

201 Rnq1 does not normally participate in spindle pole dynamics, but it does assemble at the IPOD

202 ved caspase-related protease separase (extra spindle poles [ESP]) is required for the establishment o

203 The caspase-related protease separase (EXTRA SPINDLE POLES, ESP) plays a major role in chromatid disj

204 tension to promote the coalescence of early spindle pole foci that produces a bipolar structure duri

205 Ciliobrevins also prevent spindle pole focusing, kinetochore-microtubule attachmen

206 e oscillatory movements to and away from the spindle pole for 1-2 h, so we could trap kinetochores mu

207 llel microtubule cross-linking to help focus spindle poles for efficient bipolar spindle assembly.

208 kpoint functions, centrosome number control, spindle pole formation, Slug expression and satellite RN

209 defective centrosome maturation and aberrant spindle pole formation, thus impairing the formation of

210 Overall, the model shows that abnormal spindle-pole formation and its aberrant coordination wit

211 g between kinetochore-spindle attachment and spindle-pole formation in SAC silencing.

212 In PtK2 cells 8-mW trap power stopped the spindle pole from moving toward the equator.

213 ggered simultaneous aMT organization by both spindle poles from the outset and led to symmetric conta

214 s represent APC-independent roles for EB1 in spindle pole function and the regulation of cortical con

215 asses of chromatin thus ended up at opposite spindle poles, giving the appearance of successful anaph

216 cellular MTOCs progressively decreased, the spindle pole gradually became more focused, and spindle

217 e fusion protein, which localizes to mitotic spindle poles, has constitutive kinase activity and indu

218 Aurora B kinase (ABK), typically occurs near spindle poles; however, the relevance of this locale is

219 romosomes tend to misalign toward the oldest spindle pole in a cenexin- and PLK1-dependent manner.

220 extracts, we show that SSX2IP accumulated at spindle poles in a Dynein-dependent manner and interacte

221 es then separate at the same velocity as the spindle poles in an anaphase B-like movement.

222 over, our mathematical model showed that two spindle poles in close proximity do not "search" the ent

223 ion with astral MTs and concentration around spindle poles in early M-phase.

224 ites, focusing microtubule minus ends to the spindle poles in early mitosis, and is implicated in mai

225 entrosomes are clustered into two functional spindle poles in many cancer cells.

226 ed protein that colocalizes with MAD1 at the spindle poles in metaphase and anaphase.

227 ence protein, can colocalize with RED at the spindle poles in prometaphase, and their expression can

228 and CENP-E transported chromosomes away from spindle poles in random directions.

229 ly, accumulation of checkpoint components at spindle poles increases markedly only when every kinetoc

230 e inner core amplified along with the unique spindle pole, indicating the inner core and spindle pole

231 Importantly, restoring mitotic spindle pole integrity following centromere inactivation

232 portant for centrosome number regulation and spindle pole integrity specifically in mES cells.

233 ation of centromere function impacts mitotic spindle pole integrity.

234 some alignment at the metaphase plate and in spindle pole integrity.

235 Therefore, stabilization of the centrosome-spindle pole interface by the CEP215-HSET complex could

236 bryos are also defective at coalescing extra spindle poles into a bipolar spindle.

237 Pkl1 localization and function at the spindle pole is mutually dependent on spindle pole-assoc

238 interaction between centrosomes and mitotic spindle poles is important for efficient spindle formati

239 ynein recruitment to the nuclear surface and spindle poles is severely reduced in Lis-1 male germ cel

240 of this organelle in organizing the mitotic spindle poles is well established, its precise contribut

241 s attach to microtubules (MTs) from opposing spindle poles, is the configuration that best ensures eq

242 if2a-depleted oocytes were also defective in spindle pole localization of gamma-tubulin and showed sp

243 Spindle pole localization of WDR62 and mitotic progressi

244 astral microtubules, restores gamma-tubulin spindle pole localization, and generates robust spindles

245 Not only do these data reveal a spindle-pole-localized complex for spindle orientation,

246 Astral microtubules grow out from the two spindle poles, make contact with the cell cortex, and th

247 This suggests a role for Rab11 activity in spindle pole maturation during mitosis.

248 ism for aneuploidy in fission yeast based on spindle pole microtubule defocusing by loss of kinesin-1

249 chromosomes with kinetochores unattached to spindle pole microtubules.

250 s, and its knockdown results in an unfocused spindle pole morphology and a disruption of proper spind

251 nd RAN, while full-length OCLN loss impaired spindle pole morphology, astral and mitotic microtubule

252 s find that large cytoplasmic volume affects spindle pole morphology, chromosome alignment, and strin

253 ghter cells by dynein-dependent differential spindle pole motion in anaphase.

254 Bud6p couples MT growth and shrinkage with spindle pole movement relative to the contact site.

255 e development of mitotic aster asymmetry and spindle pole movement towards the subdomain of cortical

256 itoses that showed the severest asymmetry in spindle pole movement.

257 se-specific cortical blebbing and asymmetric spindle pole movement.

258 Instead, MAPKBP1 is recruited to mitotic spindle poles (MSPs) during the early phases of mitosis

259 of a unique set of centrosome proteins from spindle poles (ninein, Cep215, centriolin).

260 ove at the same velocity as outwardly moving spindle poles, or both.

261 Further, we show that these spindle pole-positioning roles are independent of the CL

262 e are attached to microtubules from opposite spindle poles prior to chromosome segregation at anaphas

263 of mutations in genes encoding centrosome or spindle pole proteins that cause autosomal recessive pri

264 rotubules, delays mitosis, and redistributes spindle pole proteins.

265 scaffold for this functionally linked set of spindle pole proteins.

266 e microtubules (kMTs), which extend from the spindle pole region to kinetochores assembled upon centr

267 nal NuMA, microtubules lose connection to MI spindle poles, resulting in highly disorganized early sp

268 d the dissociation of gamma-tubulin from the spindle poles, resulting in severely defective spindles

269 This activity limits the extent to which spindle poles separate, leading to transient spindle len

270 ule polymerization are sufficient to promote spindle pole separation and the assembly of bipolar spin

271 w that Kif15 can assume a commanding role in spindle pole separation as a consequence of its mislocal

272 ion in cells with complete versus incomplete spindle pole separation at NEB.

273 are microtubule-dependent motors that drive spindle pole separation during mitosis.

274 to be achieved through a mechanism in which spindle pole separation forces from plus-end-directed mo

275 iation without affecting Mud accumulation at spindle poles, suggesting phosphorylation acts as a mole

276 teins colocalize on spindles and move toward spindle poles, suggesting that they form a complex.

277 e more astral microtubules on one of the two spindle poles than the other.

278 nd grow dramatically, each forming a nascent spindle pole that nucleates a radial array of microtubul

279 ile ring forms on the cortex adjacent to one spindle pole, then ingresses down the length of the spin

280 onizes the kinesin-13 depolymerase KLP10A at spindle poles, thereby switching off the depolymerizatio

281 ched to microtubules emanating from the same spindle pole, they activate another mechanosensitive mec

282 ow that clathrin remains associated with the spindle poles throughout mitosis and that this clathrin

283 e organization and then redistributes to the spindle pole to ensure faithful spindle architecture.

284 tachment errors, kinetochore-MTs detach near spindle poles to allow formation of correct attachments.

285 g force on microtubules that extend from the spindle poles to the cell cortex, thereby displacing the

286 ely promotes chromosome congression from the spindle poles to the metaphase plate.

287 l concentrations of p60, like those found on spindle poles, to maintain severing activity while most

288 Once the spindle pole-to-kinetochore contact has been made, the h

289 ces from minus-end-directed motors that pull spindle poles together.

290 e, two to three microtubules extend from the spindle pole toward the cell cortex.

291 d that PLK1 asymmetrically localizes between spindle poles under conditions of chromosome misalignmen

292 MT minus-ends are anchored by centrosomes at spindle poles, we hypothesized that the MT minus-end mot

293 Nevertheless, spindle poles were formed after one hour of mitotic rele

294 es revealed that high NuMA levels at mitotic spindle poles were significantly associated with a decre

295 t mechanism that "engages" to push apart the spindle poles when poleward flux is turned off.

296 csi2p localizes to the spindle poles, where it regulates mitotic microtubule dy

297 tubule pairing is sufficient to separate the spindle poles, whereas interpolar microtubules maintain

298 facilitates the assembly of the two mitotic spindle poles, which are required for the formation of t

299 aching to dynamic microtubules from opposite spindle poles, which exerts forces to bring kinetochores

300 astral microtubule-mediated forces align the spindle poles with cortical cues parallel to the substra