ライフサイエンスコーパス: ssDNA

1 ssDNA binding by the GRF-ZF motifs helps recruit NEIL3 t

2 ssDNA is determined to adsorb strongly to no-oxidation G

3 ssDNA-GQD complexation is confirmed by atomic force micr

4 competitively displacing (GT)(6) vs (GT)(15) ssDNA from ssDNA-SWCNTs.

5 ukaryotic chromosomes typically contain a 3' ssDNA G-rich protrusion (G-overhang).

6 ssDNA binding, ssDNA endonuclease, 5' to 3' ssDNA translocase and 5' to 3' helicase activity.

7 resolves SsbA tetramers joined together by a ssDNA "bridge" and identifies an interface, termed the "

8 force microscopy, we show that CMG harbors a ssDNA gate that enables transitions between ss and dsDNA

9 id structure) for cutting and rejoining of a ssDNA strand can be combined with two different types of

10 Only a ssDNA-RecA filament supported LexA cleavage.

11 ified single-stranded deoxyribonucleic acid (ssDNA) aptamer was specially designed and synthesized to

12 odified single-strand deoxyribonucleic acid (ssDNA) as anchored probe and 6-Mercapto-1-hexanol (MCH)

13 cally single-stranded deoxyribonucleic acid (ssDNA) encapsulated within 25-nm protein capsids.

14 move them with CRISPR-Cas12a, a hyper-active ssDNA endonuclease without sequence specificity.

15 ing a method that maps blunt-ended DNA after ssDNA digestion.

16 n a previous NMR study, SP_0782 exhibited an ssDNA-binding property different from YdbC, a prokaryoti

17 Our results indicate that RADX is an ssDNA condensation protein that inhibits RAD51 filament

18 -term intrahost evolutionary processes of an ssDNA virus that emerged to cause a pandemic in a new ho

19 tching in genome analysis, here we report an ssDNA stretching platform: two dimensional in-plane hete

20 , with the formation of antigen-antibody and ssDNA/oligonucleotide-complementary strand complexes in

21 ard was used to calibrate the detection, and ssDNA dilutions were qPCR-amplified to obtain a standard

22 to be polyreactive against LPSs, dsDNA, and ssDNA.

23 ization of BRCA2 is counteracted by DSS1 and ssDNA.

24 nteraction of BRCA2 is sensitive to DSS1 and ssDNA.

25 ent-strand degradation at reversed forks and ssDNA accumulation.

26 ll regulated by the stoichiometry of SSB and ssDNA.

27 copy in discerning intramolecular (ssRNA and ssDNA) and intermolecular (RNA-RNA, RNA-DNA, and DNA-DNA

28 ctions and possesses ssDNA-dependent ATPase, ssDNA binding, ssDNA endonuclease, 5' to 3' ssDNA transl

29 sly undescribed recombination events between ssDNA and ssRNA viruses.

30 pical Escherichia coli SSB tetramer can bind ssDNA using multiple modes that differ by the number of

31 creases with the number of domains that bind ssDNA primarily with conserved aromatic residues and pos

32 ations, it dissociates into dimers that bind ssDNA with high affinity.

33 we confirm that RPA inhibits A3A by binding ssDNA, but despite its overexpression following cisplati

34 in protein and found that it prefers binding ssDNA and ssRNA.

35 and slows DNA end resection through binding ssDNA.

36 esses ssDNA-dependent ATPase, ssDNA binding, ssDNA endonuclease, 5' to 3' ssDNA translocase and 5' to

37 DC1 (known to localize in the nucleus) binds ssDNA containing N6mA, with a 10 nM dissociation constan

38 To perform its functions, RPA binds ssDNA tightly.

39 Our data suggests A3G securely binds ssDNA through the NTD, while the CTD samples and potenti

40 ein (SSB) is an essential protein that binds ssDNA intermediates formed during genome maintenance.

41 IP, an OB-fold containing protein that binds ssDNA, as a DNA repair factor involved in HR.

42 tant protein forms filaments in vitro, binds ssDNA cooperatively, and stimulates the activities of ot

43 The mobilities of both ssDNA and dsDNA decrease with increasing ionic strength

44 -N terminal self-interaction is modulated by ssDNA.

45 via higher-order assemblies that can capture ssDNA in trans.

46 In BRCA-deficient cells, ssDNA gaps developed because replication was not effecti

47 Biological production of circular ssDNA (cssDNA) using M13 addresses these needs at low co

48 pSGN system required less nuclease to cleave ssDNA substrates than the SGN system we reported previou

49 s (PAM); is a multi-turnover enzyme; cleaves ssDNA, dsDNA and RNA targets in a single assay; and oper

50 amenable to strand invasion by RAD51-coated ssDNA filaments.

51 ongly promotes the degradation of RPA-coated ssDNA by DNA2.

52 RADX compacts RPA-coated ssDNA filaments via higher-order assemblies that can cap

53 e other ssDNA-binding proteins on RPA-coated ssDNA.

54 tate annealing of the overhang to SSB-coated ssDNA at the replication fork.

55 promote efficient annealing of complementary ssDNA and is thus considered to be a member of the SSAP

56 eins in vitro, we reveal that RADX condenses ssDNA filaments, even when the ssDNA is coated with RPA

57 SSBs share a conserved ssDNA-binding domain, a less conserved intrinsically dis

58 in of YTHDC1 in complex with N6mA-containing ssDNA, which illustrated that YTHDC1 binds the methylate

59 he writer-reader-eraser of N6mA-containining ssDNA is associated with maintaining genome stability.

60 ing of the mechanisms underlying cooperative ssDNA binding by SSBs has been hampered by the limited a

61 ch is to assist RAD51 loading on RPA-covered ssDNA.

62 onuclei and with the presence of cytoplasmic ssDNA, leading to the activation of the IFI16/STING path

63 p44/p62's high affinity (20 nM) for damaged ssDNA.

64 ructures of SP_0782 complexed with different ssDNAs reveal that the varied binding patterns are assoc

65 sting of an RNA-DNA hybrid and the displaced ssDNA strand.

66 characterized, despite the fact that diverse ssDNA bacteriophages have been discovered via metagenomi

67 ion intermediates comprising Rad51- and Dmc1-ssDNA in real time.

68 d that yeast Hop2-Mnd1 bound rapidly to Dmc1-ssDNA filaments with high affinity and remained bound fo

69 DNA aptamers are single-strand DNA (ssDNA) capable of selectively and tightly binding a targ

70 er genomes were linked to single-strand DNA (ssDNA) intermediates in various processes of DNA metabol

71 after cleavage of the 5' single-strand DNA (ssDNA) tail by the AdnA nuclease.

72 ediating interaction with single-strand DNA (ssDNA), whereas the major AP endonuclease APE1 does not.

73 results from its lower single-stranded DNA (ssDNA) affinity, compared to that of ScRad51.

74 a helical filament with single-stranded DNA (ssDNA) and ATP.

75 ion of RPA, a sensor of single-stranded DNA (ssDNA) and DNA replication stress.

76 ion mobilities of small single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) have been measure

77 deaminate cytosines in single-stranded DNA (ssDNA) and play key roles in innate and adaptive immunit

78 ing oxidized bases from single-stranded DNA (ssDNA) and unhooking interstrand cross-links (ICLs) at f

79 methods that can detect single-stranded DNA (ssDNA) are utilized to identify the presence, location,

80 rectly onto the initial single-stranded DNA (ssDNA) at a 3'-overhang, and second in binding to SSB to

81 tions together with the single-stranded DNA (ssDNA) binding protein RPA as the central scaffold to en

82 hat begins at a distant single-stranded DNA (ssDNA) break and proceeds back past the mismatched nucle

83 1 polymerises faster on single-stranded DNA (ssDNA) compared to double-stranded DNA (dsDNA), raising

84 APOBEC3G (A3G) is a single-stranded DNA (ssDNA) cytosine deaminase that can restrict HIV-1 infect

85 like (APOBEC) family of single-stranded DNA (ssDNA) cytosine deaminases provides innate immunity agai

86 IV-1) infectivity, is a single-stranded DNA (ssDNA) deoxycytidine deaminase with two domains, a catal

87 the formation of the 3' single-stranded DNA (ssDNA) filament needed for recombination, from yeast to

88 ocesses in viruses with single-stranded DNA (ssDNA) genomes.

89 potentially binds with single-stranded DNA (ssDNA) in a manner similar to human PC4, the prototype o

90 process that generates single-stranded DNA (ssDNA) in the genome as 'transcription bubbles'.

91 Accumulation of single-stranded DNA (ssDNA) in the lagging-strand template greatly increases

92 rating deoxyuridine, in single stranded DNA (ssDNA) intermediates produced during HIV replication.

93 n of large stretches of single-stranded DNA (ssDNA) intermediates that are rapidly protected by singl

94 substrate can resolve a single-stranded DNA (ssDNA) molecule with a spatial resolution below 1 nm.

95 Single-stranded DNA (ssDNA) molecules in solution typically form coiled struc

96 s increasing demand for single-stranded DNA (ssDNA) of lengths >200 nucleotides (nt) in synthetic bio

97 omes and terminate in a single-stranded DNA (ssDNA) overhang recognized by POT1-TPP1 heterodimers to

98 free DNA ends to expose single-stranded DNA (ssDNA) overhangs.

99 nment, only a few lytic single-stranded DNA (ssDNA) phages have been isolated and characterized, desp

100 up to 1-kb inserts when single-stranded DNA (ssDNA) repair templates are supplied.

101 degrades a fluorescent single stranded DNA (ssDNA) reporter present in the assay.

102 A synthetic GAPDH single-stranded DNA (ssDNA) standard was used to calibrate the detection, and

103 RPA, RAD51, and DMC1 to single-stranded DNA (ssDNA) substrates created after formation of programmed

104 was functionalized with single-stranded DNA (ssDNA) template (T30), spaced with hexanedithiol (HDT) i

105 nce for BLM activity on single-stranded DNA (ssDNA) that is bound by replication protein A (RPA).

106 Pif1 is also known as a single-stranded DNA (ssDNA) translocase, while how ScPif1 translocates on ssD

107 Single-stranded DNA (ssDNA) viruses appear to blend both strategies, using nu

108 Geminiviruses are single-stranded DNA (ssDNA) viruses that infect a wide range of plants.

109 production of kilobase single-stranded DNA (ssDNA) with sequence control has applications in therape

110 Csm can cleave RNA and single-stranded DNA (ssDNA), but whether it targets one or both nucleic acids

111 ation and the displaced single-stranded DNA (ssDNA), have been identified in bacteria, yeasts, and ot

112 electrical detection of single-stranded DNA (ssDNA), in-solution- and on-chip-hybridized double-stran

113 rge-scale production of single-stranded DNA (ssDNA), we probed the substrate specificity, mutation sp

114 CST bound to telomeric single-stranded DNA (ssDNA), which assembles as a decameric supercomplex.

115 g of Sgs1 activities on single stranded DNA (ssDNA), which is a central intermediate in all aspects o

116 Mitochondrial single-stranded DNA (ssDNA)-binding proteins (mtSSBs) are required for mitoch

117 In this process, a single-stranded DNA (ssDNA)-RecA nucleoprotein filament invades homologous ds

118 zyme that contains both single-stranded DNA (ssDNA)-specific nuclease and motor activities.

119 ' direction, generating single-stranded DNA (ssDNA).

120 ith a specific focus on single-stranded DNA (ssDNA).

121 e demonstrate that gp14, termed here as Drc (ssDNA-binding RNA Polymerase Cofactor), preferentially b

122 ng duplex DNA in a naturally occurring dsDNA-ssDNA telomere interface using polyamide (PA) and pyrido

123 ting strands form ssDNA-RecA filaments, each ssDNA-RecA filament searches for homologous double-stran

124 In particular, the circular, Rep-encoding ssDNA (CRESS-DNA) viruses show high diversity and abunda

125 lication protein A (RPA), a major eukaryotic ssDNA-binding protein, is essential for all metabolic pr

126 this signaling pathway results in excessive ssDNA, chromosomal instability, and hypersensitivity to

127 tion of this protein pool to protect exposed ssDNA and repair genomic loci affected by DNA damage.

128 suggest that ScPif1 translocates on extended ssDNA in two distinct modes, primarily in a 'mobile' man

129 cs, which is rarely accompanied by extensive ssDNA exonuclease digestion.

130 For ssDNA, the predicted mobilities are close to the observe

131 species is proposed to be the one active for ssDNA binding.

132 he C-terminal region has higher affinity for ssDNA than the N-terminal active site.

133 believed to provide additional affinity for ssDNA.

134 the AuNPs create a suitable environment for ssDNA immobilization.

135 ucial before applying any nanotechnology for ssDNA analysis.

136 tes with complex structures including forked ssDNA overhangs and nucleoprotein conjugates.

137 Once the initiating strands form ssDNA-RecA filaments, each ssDNA-RecA filament searches

138 and bacteriophage T7 DNA polymerases on free-ssDNA, in comparison with ssDNA covered with homologous

139 ariants are also more readily displaced from ssDNA by RecA than wild-type SSB.

140 an only process small peptide fragments from ssDNA ends, raising the question of how the ~90 kDa TOP1

141 by which tightly bound SSBs are removed from ssDNA by the lagging strand DNA polymerase without compr

142 ly displacing (GT)(6) vs (GT)(15) ssDNA from ssDNA-SWCNTs.

143 Furthermore, ssDNA appears to staple two monomers to nucleate decamer

144 We found that two processes generate ssDNA that could support LexA cleavage.

145 nce of blocking oligonucleotides, generating ssDNA fragments capable of hybridizing with oligonucleot

146 Hence, ssDNA and dsDNA appear to interact in a similar manner w

147 ancer tolerance to formation of hypermutable ssDNA is similar to yeast and that the predominant patte

148 rmed that the rates of temperature-driven iM-ssDNA transitions correlate with the rates of the pH-dri

149 action between N(3)-kethoxal and guanines in ssDNA.

150 that the TC target was strongly preferred in ssDNA regions rather than dsDNA, loop or bulge regions,

151 have lost the spike protein commonly seen in ssDNA phages, suggesting that ssDNA phage can be more di

152 s that HMCES has specificity for AP sites in ssDNA at junctions found when replicative polymerases en

153 and mutational signature of redox stress in ssDNA of budding yeast and the signature of aging in hum

154 e show that UdgX strongly prefers uracils in ssDNA over those in U*G or U:A pairs, and localizes to n

155 This phenomenon may be widespread in ssDNA viruses that simultaneously synthesize and package

156 rmed by atomic force microscopy, by inducing ssDNA desorption, and with molecular dynamics simulation

157 ial for all metabolic processes that involve ssDNA, including DNA replication, repair, and damage sig

158 phage Mini is distantly related to the known ssDNA phages and belongs to an unclassified ssDNA phage

159 ermediates, such as reversed forks that lack ssDNA.

160 ests that an OB fold in Dbp2 directs leading ssDNA from CMG to the Pol epsilon active site.

161 bout molecular mechanisms that generate long ssDNA vulnerable to hypermutation.

162 ely impaired the heterodimer binding to long ssDNA substrates containing multiple protein binding sit

163 In contrast, the lytic ssDNA and ssRNA phages have a single lysis protein that

164 at in suppressing APE1 endonuclease-mediated ssDNA breakage.

165 Mitochondrial ssDNA-binding protein also increased the estimated trans

166 guration well-suited for binding to multiple ssDNA conformations.

167 rsely, SN2-type agents preferably mutagenize ssDNA in yeast.

168 nctionalized single-walled carbon nanotubes (ssDNA-SWCNTs), a nanoparticle used widely for sensing an

169 s study, we isolated and characterized a new ssDNA phage, vB_RpoMi-Mini, which infects a marine bacte

170 NMR ssDNA-binding experiments revealed that the interaction

171 Csm (SepCsm) cleave RNA transcripts, but not ssDNA, at the transcription bubble.

172 rk has focused on retroviruses with obligate ssDNA replication intermediates and it is unclear whethe

173 from chromatin, resulting in the absence of ssDNA accumulation, RPA binding, and activation of the A

174 ify the presence, location, and abundance of ssDNA on mtDNA.

175 PRIMPOL repriming leads to accumulation of ssDNA gaps while suppressing fork reversal.

176 We propose that accumulation of ssDNA in the lagging-strand template fosters the formati

177 Adsorption of ssDNA quenches intrinsic GQD fluorescence by 31.5% for l

178 of heteroduplex pairing with the binding of ssDNA to the secondary site, to extend dsDNA opening.

179 eutic effects mainly through the blockade of ssDNA damage repair, which leads to the accumulation of

180 cursors further caused increased exposure of ssDNA associated with disruption of genome fragile sites

181 A3G stabilizes formation of ssDNA loops, an ability inhibited by A3G oligomerization

182 Iterative rounds of nTET hydroxylations of ssDNA proceeded with high stereo specificity and include

183 tructures can be controlled by the length of ssDNA overhangs positioned adjacent to the cholesterol.

184 This mechanism locally limits the length of ssDNA sampled for pairing if homology is not encountered

185 ed binding patterns for different lengths of ssDNA, and tends to form large complexes with ssDNA in a

186 -ends of the crRNA with different lengths of ssDNA, ssRNA, and phosphorothioate ssDNA, we discover a

187 Increased levels of ssDNA, reduced levels of Cse4 and its assembly factor Sc

188 itive and genome-wide capture and mapping of ssDNA produced by transcriptionally active RNA polymeras

189 Remarkably, Poltheta MMEJ of ssDNA overhangs requires polymerase-helicase attachment,

190 In the presence of ssDNA, Rep68 forms a large double-octameric ring complex

191 dicates our dearth of knowledge regarding of ssDNA phages.

192 Given the significance of ssDNA stretching in genome analysis, here we report an s

193 d been clearly associated with a subclass of ssDNA-specific apolipoprotein B mRNA editing enzyme, cat

194 he mobility of dsDNA is greater than that of ssDNA at all ionic strengths because of the greater char

195 l force, we identified two distinct types of ssDNA translocation.

196 f vB_RpoMi-Mini expands our understanding of ssDNA phages in nature, and also indicates our dearth of

197 er reveal that APE1 endonuclease activity on ssDNA but not on dsDNA is compromised by a NEIL3 Zf-GRF

198 astly, we study real-time protein binding on ssDNA-SWCNTs, obtaining agreement between enriched prote

199 ge assay to study protein corona dynamics on ssDNA-SWCNT-based dopamine sensors.

200 atically assessing RAD51 binding kinetics on ssDNA and dsDNA differing in length and flexibility usin

201 ement by RAD51 and prevents RAD51 loading on ssDNA.

202 RAD51 have distinct spatial localization on ssDNA: DMC1 binds near the break site, and RAD51 binds a

203 n activity of individual ScPif1 molecules on ssDNA extended by mechanical force, we identified two di

204 nterfaces that link adjacent SSB proteins on ssDNA.

205 en coupled to DNA polymerase, CMG remains on ssDNA, but when uncoupled, CMG employs this gate to trav

206 while ScRad51 nucleation depends strongly on ssDNA lengths.

207 ranslocase, while how ScPif1 translocates on ssDNA is unclear.

208 on, critical for Exo1 activation on not only ssDNA but also dsDNA.

209 facilitates the polymerization of RAD51 onto ssDNA to form a presynaptic nucleoprotein filament.

210 ric structure suggests that CST can organize ssDNA analogously to the nucleosome's organization of do

211 filament formation and may antagonize other ssDNA-binding proteins on RPA-coated ssDNA.

212 ntains a structural fold distinct from other ssDNA-binding proteins (SSBs).

213 und that RAD51 in stoichiometric excess over ssDNA can cause D-loop disassembly in vitro; furthermore

214 ucleic acid sequencing using TERS of a phage ssDNA (M13mp18).

215 engths of ssDNA, ssRNA, and phosphorothioate ssDNA, we discover a self-catalytic behavior and an augm

216 recognises ss-dsDNA junctions and possesses ssDNA-dependent ATPase, ssDNA binding, ssDNA endonucleas

217 in treatment, RPA is unable to fully protect ssDNA created by cisplatin adducts.

218 rand (ss) DNA binding (SSB) protein protects ssDNA intermediates and recruits at least 17 SSB interac

219 , combined biophysical analysis of the RAD51-ssDNA interaction with mathematical modeling to show tha

220 e for an association of Hop2-Mnd1 with Rad51-ssDNA or RPA-ssDNA.

221 the presence of SSB and RecA, a stable RecA-ssDNA filament is not formed, although sufficient RecA*

222 also without the generation of a stable RecA-ssDNA filament.

223 We demonstrate that the recovered ssDNA can be used for CRISPR/Cas9 homology directed repa

224 ed the cryo-EM and X-ray structures of Rep68-ssDNA complexes.

225 entropic penalty associated with restricting ssDNA flexibility is offset by a strong RAD51-RAD51 inte

226 The resulting ssDNA is rapidly bound by RPA, which further stimulates

227 ciation of Hop2-Mnd1 with Rad51-ssDNA or RPA-ssDNA.

228 synthetic molecular recognition by screening ssDNA-wrapped SWCNTs with different sequences against a

229 Using a sensor array of select ssDNA wrappings, we are able to distinguish between Cu(I

230 In selecting ssDNA over dsDNA, the RAD51 DNA strand exchange protein

231 ificantly reduced POT1-TPP1 binding to short ssDNA substrates; however, it only moderately impaired t

232 ssociated with a higher affinity for shorter ssDNA than one single Zf-GRF motif.

233 cation for the generation of the SOS signal, ssDNA.

234 Titration of small ssDNA oligonucleotides to Mycobacterium smegmatis topois

235 MEIOB, a meiosis-specific ssDNA-binding protein, regulates early meiotic recombina

236 SSB, ssDNA, and SSB-interacting molecules are highly concentr

237 RecO binding to the SSB tetramer and an SSB-ssDNA complex show significant thermodynamic differences

238 ecA filament formation on the leading-strand ssDNA gaps generated by replisome lesion skipping.

239 omerases interact with G-strand and T-strand ssDNA to regulate DNA topology.

240 ow that with a biasing voltage the stretched ssDNA can be electrophoretically transported along the "

241 form coiled structures, therefore stretching ssDNA is extremely crucial before applying any nanotechn

242 (CTD) and a catalytically inactive, strongly ssDNA binding N-terminal domain (NTD).

243 distinguishes the binding mode of substrate ssDNA from non-substrate.

244 ding domain (XPA DBD) and the RPA70AB tandem ssDNA-binding domains, which is likely to influence the

245 resistance after hybridization with a target ssDNA specific of Coxsackie B3 virus was monitored using

246 d then base pairing between hTR and telomere ssDNA promotes long interactions required for stable tel

247 changes involved in the assembly of telomere ssDNA substrates of differing lengths bound by POT1-TPP1

248 physiologically relevant lengths of telomere ssDNA.

249 266 of POT1 that were dependent on telomere ssDNA substrate length.

250 bined with two different types of C-terminal ssDNA binding domains to form diverse bacterial topoisom

251 We demonstrate that ssDNA is recoverable in ~40-50 min (time after PCR) with

252 Together, these data indicate that ssDNA replication gaps underlie the BRCA cancer phenotyp

253 exagonal boron nitride (h-BN), and show that ssDNA can be stretched on a h-BN nanostripe sandwiched b

254 Here, we show that ssDNA intermediates formed during the repair of gamma-in

255 Here, we show that ssDNA replication gaps underlie the hypersensitivity of

256 The results suggest that ssDNA strands exist as an ensemble of relatively compact

257 mmonly seen in ssDNA phages, suggesting that ssDNA phage can be more diverse than previously thought.

258 This study suggests that ssDNA replication gaps are fundamental to the toxicity o

259 The ssDNA-binding protein SSB1 has been described in human c

260 ical temperature and continues to cleave the ssDNA reporter even after 24 h of incubation, resulting

261 applied qPCR-based analyses to evaluate the ssDNA pool size and remelting curve analysis of qPCR amp

262 This study provides insights into the ssDNA-binding mechanism of PC4-like proteins, and benefi

263 n of the first responder to DNA lesions, the ssDNA-binding protein complex replication protein A (RPA

264 an evolutionarily conserved surface near the ssDNA-binding site.

265 thin a zinc-binding domain in PriA) near the ssDNA-dsDNA junction of the lagging strand in a PriA-DNA

266 When exchange reaches the 3' end of the ssDNA, a DNA polymerase can add nucleotides onto the end

267 ported RPA activity that strips Exo1 off the ssDNA.

268 lament invades homologous dsDNA, pairing the ssDNA with the complementary strand in the dsDNA.

269 ion is the 53BP1 protein, which recruits the ssDNA-binding REV7-Shieldin complex to favor C-NHEJ repa

270 Indeed, HNRNPD silencing reduced: the ssDNA fraction upon camptothecin treatment; AsiSI-induce

271 ADX condenses ssDNA filaments, even when the ssDNA is coated with RPA at physiological protein ratios

272 Mutational analysis suggests that while the ssDNA-binding channel is important for helicase activity

273 is itself modulated by competition with the ssDNA-binding protein (SSB) for binding to ssDNA.

274 plementary strand to sample pairing with the ssDNA.

275 l fold similar to those of PC4 and YdbC, the ssDNA length occupied by SP_0782 is shorter than those o

276 BDs) in RPA promote high-affinity binding to ssDNA yet also allow RPA displacement by lower affinity

277 r multimer formation, but not the binding to ssDNA.

278 In vitro, ZPET inhibits MRE11 binding to ssDNA.

279 alkylation of RAD51 inhibited its binding to ssDNA.

280 e ssDNA-binding protein (SSB) for binding to ssDNA.

281 RecA binds to ssDNA, forming filaments that stimulate proteolytic clea

282 RPA bound to ssDNA also represents a barrier, explaining the need for

283 of shieldin or forced targeting of PALB2 to ssDNA in BRCA1(D11)53BP1(S25A) cells restores RNF168 rec

284 lation damage conferred by dsDNA relative to ssDNA has not been quantified.

285 en the higher abundance of dsDNA relative to ssDNA, these results suggest that dsDNA could be a subst

286 For the detection, two ssDNA aptamers specific to the corresponding PLDH and Pf

287 However, simultaneous binding of two ssDNA segments to a type IA topoisomerase has not been o

288 ssDNA phages and belongs to an unclassified ssDNA phage within the Microviridae family.

289 The subsequent cleavage of unprotected ssDNA has been termed replication catastrophe.

290 ed DNA substrates and stabilizes the unwound ssDNA product, resulting in a ~5-fold stimulation of the

291 DNA-processing enzymes, which typically use ssDNA binding to enhance catalytic activity, and suggest

292 two domains, a catalytically active, weakly ssDNA binding C-terminal domain (CTD) and a catalyticall

293 s the 65-site-size ((SSB)(65)) mode in which ssDNA wraps completely around the tetramer is favored at

294 olymerases on free-ssDNA, in comparison with ssDNA covered with homologous and non-homologous SSBs un

295 ructures of PC4-like proteins complexed with ssDNA reveals a divergence in the binding interface betw

296 sDNA, and tends to form large complexes with ssDNA in a potential high-density binding manner.

297 as essential to a critical interaction with ssDNA.

298 n (GT)(6) and that fibrinogen interacts with ssDNA-SWCNTs more strongly than albumin.

299 of multimerized VEGF(165) aptamer joint with ssDNA based linker in the middle and poly thymine sequen

300 The toxin overloads the RPA pathway with ssDNA substrate, causing RPA exhaustion and senescence.

301 al structure of a type IA topoisomerase with ssDNA segments bound in opposite polarity to the N- and

302 conditions for the immobilization of a ZIKV ssDNA probe on the electrode surface (ox-GCE-[AuNPs-SiPy