ライフサイエンスコーパス: ssRNA

1 ssRNA binding, but not ssDNA, induces higher-order oligo

2 ssRNA can interact with dendritic cells (DCs) through bi

3 ssRNA is channeled through its multisubunit ring-like co

4 Furthermore, HIV-1 ssRNA induced histone 4 acetylation at the TNFalpha prom

5 In the current study, HIV-1 ssRNA induced TNFalpha release in a dose-dependent manne

6 mulated with HIV-1 infectious virions, HIV-1 ssRNA, TLR 7 and 8 agonists, or IFN-alpha.

7 knockdown with relevant siRNA reduced HIV-1 ssRNA-mediated TNFalpha release, but silencing TLR7 had

8 entering the substrate from the extended 3' ssRNA terminus.

9 re from sequence, constructing equivalent 3D ssRNA models, transforming the 3D ssRNA models into ssDN

10 ivalent 3D ssRNA models, transforming the 3D ssRNA models into ssDNA 3D structures, and refining the

11 double-strand regions and extended 3' or 5' ssRNA overhangs in the adjacent ssRNA regions.

12 In the presence of a ssRNA target containing adjacent sequences complementary

13 Rhinovirus (RV), a ssRNA virus of the picornavirus family, is a major cause

14 ly arrayed coat protein (CP) protecting a (+)ssRNA.

15 ded 3' or 5' ssRNA overhangs in the adjacent ssRNA regions.

16 suggesting that it should be similar for all ssRNA viruses with a comparable ratio of capsid size/gen

17 the genome is indeed a primary factor among ssRNA viruses' evolutionary constraints, contributing al

18 An ssRNA analog induced TNF-alpha and IFN-gamma-induced pro

19 mplex from Thermotoga maritima can cleave an ssRNA target that is complementary to the CRISPR RNA.

20 and obtain different RT-PCR patterns for an ssRNA knot and circle of the same sequence.

21 al gene transfer might have occurred from an ssRNA virus to a dsRNA virus, which may provide new insi

22 HCV is an ssRNA virus, which suggests a role for Toll-like recepto

23 Qbeta, an ssRNA phage specific for the conjugative F-pilus, has a

24 creases in IL-6 following incubation with an ssRNA ligand, as well as TLR8 RNA and protein expression

25 bstantial advantages of CircLigase-based and ssRNA-based capture for defining sequences and structure

26 Utilizing analogs of dsRNA (poly(I:C)) and ssRNA (ssRNA40), we demonstrated that an NLRP3-mediated

27 s into the evolutionary history of dsRNA and ssRNA viruses.

28 f single-stranded (ss) DNA geminiviruses and ssRNA viroids, respectively, but both pathogens can coun

29 ctivities that catalyze crRNA maturation and ssRNA degradation.

30 Here, we report measurements of ssDNA and ssRNA elasticity in the intermediate-force regime, corre

31 In contrast, the lytic ssDNA and ssRNA phages have a single lysis protein that achieves c

32 ribed recombination events between ssDNA and ssRNA viruses.

33 and found that it prefers binding ssDNA and ssRNA.

34 siRNAs and ssRNAs competitively inhibited human Dicer activity, sug

35 These data suggest that the siRNAs and ssRNAs interact predominantly with the PAZ domain of the

36 gene transfer between eukaryotic (+) and (-)ssRNA viruses.

37 sors of viral infection as their ligands are ssRNA and dsDNA, respectively.

38 nsfected with hY3, an anti-SSA/Ro-associated ssRNA, 2) RNA isolated from freshly sorted human leukocy

39 , whereby endogenous ligand, Ro60-associated ssRNA, forges a nexus between TLR ligation and fibrosis

40 ddressed the hypothesis that Ro60-associated ssRNAs link macrophage activation to fibrosis via TLR en

41 cytokines in strict dependence on bacterial ssRNA and the host molecules MyD88 and UNC-93B.

42 In single-stranded RNA bacteriophages (ssRNA phages) a single copy of the maturation protein bi

43 Single-stranded RNA bacteriophages (ssRNA phages) infect Gram-negative bacteria via a single

44 We find that A3A binds ssRNA, but the RNA and DNA binding interfaces differ and

45 hin the shared binding region that binds BTV ssRNA preferentially in a manner consistent with specifi

46 rovided a source of sequence-nonspecific but ssRNA-targeted in vitro ribonuclease activity that coelu

47 gonists, TLR7(rsq1) fails to be activated by ssRNA.

48 Activation of TLR by ssRNA after FcgammaR-mediated phagocytosis of immune com

49 The use of DNA-modified SiNPs to capture ssRNA for profiling has several advantages as compared t

50 Upon infection with certain ssRNA and dsRNA viruses, CHO cells fail to generate a si

51 forms of linear and covalently circularized ssRNA molecules.

52 mr effector complex has been found to cleave ssRNA in vitro.

53 tory signaling in response to a TLR8 cognate ssRNA ligand.

54 binding above that achieved by a random-coil ssRNA.

55 We also show that binding of a complementary ssRNA target activates an ssDNA-specific nuclease activi

56 the Argonaute (Ago) family to complementary ssRNA targets: RNA-guided RNA interference.

57 By using a constructed ssRNA knot as a highly sensitive topological probe, we f

58 nism through which extracellularly delivered ssRNA contributes to CNS damage and determine an obligat

59 t Nbr's EXO domain exhibits Mn(2+)-dependent ssRNA-specific 3'-to-5' exoribonuclease activity.

60 ly related to another IncP-plasmid-dependent ssRNA phage, PRR1.

61 on with TLR 7 and 8 ligands or HIV-1-derived ssRNA.

62 However, targeting any desired ssRNA remained elusive as two argonaute domains provided

63 nted proteins in the presence of any desired ssRNA target.

64 dRP that can synthesize dsRNA from different ssRNA templates using either a primer-dependent or prime

65 hogonal sets of crRNAs and possess different ssRNA cleavage specificities.

66 s13's potent activity against three distinct ssRNA viruses: lymphocytic choriomeningitis virus (LCMV)

67 Statistical co-occurrence analyses of dsDNA, ssRNA and dsRNA viral markers of polyadenylation-selecte

68 easure the stem length between the two dsRNA-ssRNA junctions.

69 nthesis and shifted the ratio of viral dsRNA/ssRNA in favor of dsRNA.

70 ing evidence for functionality of endogenous ssRNA species.

71 dependent on the expression of the endosomal ssRNA receptor TLR7.

72 ial for ssRNA cleavage, although it enhances ssRNA targeting for crRNAs encoded internally within the

73 amily Bunyaviridae), a group of enveloped (-)ssRNA viruses.

74 tation abolishes the unknotting activity for ssRNA, but not for ssDNA.

75 3a pre-crRNA processing is not essential for ssRNA cleavage, although it enhances ssRNA targeting for

76 However, many current methods for ssRNA-seq suffer from the underrepresentation of both th

77 TLR7 is the mammalian receptor for ssRNA and some nucleotide-like small molecules.

78 we propose a two-step assembly strategy for +ssRNA viruses: step I, acquisition of packaging specific

79 the structural and dynamic behavior of free ssRNAs at atomic resolution.

80 Furthermore, ssRNA-TNP uptake is dependent on macropinocytosis and cl

81 a preference of ssDNA approximately dsDNA > ssRNA, which is distinct from Rx1.

82 n these Antarctic RNA virus metagenomes had +ssRNA genomes most closely related to viruses in the ord

83 exosome-packaged HCV, cell-free HCV, or HCV ssRNA induced differentiation into MPhis with high M2 su

84 exosome-packaged HCV, cell-free HCV, or HCV ssRNA.

85 Further, we demonstrated that HCV ssRNA and other TLR7/8 ligands promote MPhi polarization

86 The HCV ssRNA-induced monocyte activation and differentiation in

87 macrophages, TLR8 binds and internalizes HIV ssRNA, leading to endosomal acidification, chromatin rem

88 , their individual roles in TLR-mediated HIV ssRNA recognition are unclear.

89 ch leads to the release of two copies of HIV ssRNA.

90 and promotes strand exchange with homologous ssRNA or ssDNA.

91 ructural and ionic signatures of homopolymer ssRNAs explains their role(s) in folding structured RNAs

92 e cooperativity in the binding to homopurine ssRNAs indicates that the type of nucleic acid base dram

93 me shows a preference for the homopyrimidine ssRNAs.

94 However, ssRNA cleavage measured by qRT-PCR underestimated inacti

95 ing guide RNA to target and inhibit a human +ssRNA virus, hepatitis C virus, within eukaryotic cells.

96 ternal autoantibodies necessary for CHB, hY3 ssRNA, and affinity-purified anti-Ro60 antibody induces

97 with 5'-end phosphorylation vastly improves ssRNA activity both in vitro and in vivo.

98 viously unappreciated level of complexity in ssRNA, which we believe will also serve as an excellent

99 like protease domain that commonly exists in ssRNA viruses, including members of the families Potyvir

100 Fluorescence properties in ssRNA are defined by a small increase in average quantum

101 various RNA containing substrates, including ssRNA, dsRNA, RNA:DNA hybrids, and R-loops.

102 ity of viruses other than AaV, including (+) ssRNA viruses.

103 ce of a fully active RNAi suppressor induces ssRNA-specific ribonuclease activity, including that con

104 used by TLR2 activation was shown to inhibit ssRNA-induced IFN expression in dendritic cells.

105 Interestingly, ssRNA-TNP is more efficient to inhibit the expression of

106 n spectroscopy in discerning intramolecular (ssRNA and ssDNA) and intermolecular (RNA-RNA, RNA-DNA, a

107 The small ~30-kb ssRNA genome of coronaviruses makes them adept at cross-

108 t that the genomes of many viruses are large ssRNA molecules-often several thousand nucleotides long-

109 ading the dying cells with the TLR7/8 ligand ssRNA, whereas dying cells loaded with TLR3 ligand were

110 tes in response to uridine-rich (viral-like) ssRNA.

111 t homologous contacts can occur between long ssRNA and dsDNA in the absence of protein and that these

112 We propose that long ssRNA interacts paranemically with long dsDNA via period

113 Bacterial, yeast, and mammalian ssRNA encapsidated within HBcAg(183) all function as TLR

114 upport a key role for autophagy in mediating ssRNA virus detection and interferon-alpha secretion by

115 Of the three methods, ssRNA capture was most effective in defining sequences t

116 te) derived from this fluorescently modified ssRNA positively signals a binding event upon interactio

117 tive routes of delivery, chemically modified ssRNAs could represent a powerful RNAi platform.

118 To date, for most ssRNA virions, only the structures of the capsids and th

119 ng signal (PS) with capsid protein(s) (most +ssRNA viruses so far studied); step II, cocondensation o

120 , and they possess diverse genomes of mostly ssRNA and dsRNA and, recently, circular ssDNA.

121 ection method can be used to detect multiple ssRNA sequences at concentrations as low as 100 fM in 50

122 Exclusion of single or multiple ssRNA segments in the packaging reaction or their additi

123 d siRNA-loaded triangular DNA nanoparticles (ssRNA-TNP).

124 tion, and also smaller than those of natural ssRNAs that are not under evolutionary pressure to have

125 The NeC3PO:ssRNA and NeC3PO:ssDNA complexes fold like closed footba

126 The NeC3PO:ssRNA structure represents the only catalytic form C3PO

127 West Nile virus (WNV) is a neurotropic ssRNA flavivirus that can cause encephalitis, meningitis

128 Applying the method to new ssRNA-seq data from whole-root and cell-type-specific Ar

129 oat protein of a plant virus and a noncoding ssRNA molecule, are highly immunogenic in mice.

130 vant consists of a 547-nt uncapped noncoding ssRNA containing polyU repeats that is stabilized by a c

131 Both macrophage transfection with noncoding ssRNA that bind Ro60 and an IC generated by incubation o

132 ict the average MLD values of large nonviral ssRNAs scale as N(0.67+/-0.01), where N is the number of

133 ed by single-stranded DNAs (ssDNAs), but not ssRNA or dsDNA.

134 mal frameshift-inducing RNA hairpin, but not ssRNAs or DNAs, at 200 mM NaCl, pH 7.5.

135 Here we report a novel ssRNA-seq method that does not involve second-strand cDN

136 Furthermore, the NP-ssRNA structures presented here, combined with hydrogen-

137 (REMD) simulations to characterize the 12 nt ssRNA tail derived from the prequeuosine riboswitch.

138 ponses triggered by the synthetic analogs of ssRNA viruses (polyuridine) and dsRNA viruses (polyinosi

139 the factors that drive efficient assembly of ssRNA viruses in vivo.

140 utionary events for these two main clades of ssRNA phages.

141 otentially leading to incomplete cleavage of ssRNA and the release of short (3-5) nucleotide products

142 te site-specific endonucleolytic cleavage of ssRNA targets, similar to PAM-mediated stimulation of Ca

143 effect also depends on the concentration of ssRNA and is abolished by overstretching of the dsDNA.

144 ed with the generation of multiple copies of ssRNA via in situ surface transcription by RNA polymeras

145 ding interfaces differ and no deamination of ssRNA is detected.

146 agnetic tweezers, we measured the effects of ssRNA on force extension curves for dsDNA.

147 btain a deeper insight into the evolution of ssRNA phages, more phages specializing for various conju

148 curately capture the inherent flexibility of ssRNA loops, accurate base stacking energetics, and puri

149 between the mutually exclusive functions of ssRNA binding and dNTP hydrolysis depending on dNTP pool

150 tial Cas13 crRNA target sites in hundreds of ssRNA viral species that can potentially infect humans.

151 order in the packaged genomes of a number of ssRNA viruses.

152 we measure the persistence lengths (l(p)) of ssRNA.

153 s of Slfn11 on the replication of a panel of ssRNA viruses in the human glioblastoma cell line A172,

154 adjacent 5-fold vertex, the entry pathway of ssRNA segments.

155 We observe that the presence of ssRNA impedes the extension of dsDNA, specifically at lo

156 3 can be harnessed to target a wide range of ssRNA viruses and CARVER's potential broad utility for r

157 This structure, involving the recognition of ssRNA via a stem-loop conformation, together with our tw

158 ce, which both have defective recognition of ssRNA, and found increased viremia and susceptibility to

159 repeat proteins can target a limited set of ssRNA sequences, there are no general methods for target

160 Much of our understanding of ssRNA conformational behavior is limited to structures i

161 and Vienna computations on large numbers of ssRNAs of various lengths (1000-10 000 nt) and sequences

162 ignificantly increased the replication of (+)ssRNA viruses from the Flavivirus genus, including West

163 isplayed robust endoribonuclease activity on ssRNA with a preference for cleavage after purine-pyrimi

164 s B virus, and simian virus 40 assembling on ssRNA and dsDNA substrates.

165 en the RNA and DNA species, and dependent on ssRNA lengths (>/=1 kb).

166 impact of specific chemical modifications on ssRNA activity implies an Ago-mediated mechanism but the

167 esium, the total site size of the polymerase-ssRNA complex is 26 +/- 2 nucleotides.

168 o repeat and expanded the scope of potential ssRNA targets.

169 y of a biocompatible gold nanorod, GNR-5'PPP-ssRNA nanoplex, as an antiviral strategy against type A

170 ctivity that was TBSV sequence-preferential, ssRNA-specific, divalent cation-dependent, and insensiti

171 TLRs irrespective of whether they recognize ssRNA, dsRNA or hypomethylated DNA.

172 rent study, the role of TLR7 that recognizes ssRNA was examined.

173 on the genome packaging of a representative ssRNA virus, the bacteriophage MS2, via a series of biom

174 study, we assessed the role of uridine-rich ssRNA derived from the HIV-1 long terminal repeat (ssRNA

175 parallel strand-specific sequencing of RNA (ssRNA-seq) has emerged as a powerful tool for profiling

176 A(n)-mediated cleavage of single-strand RNA (ssRNA) by the trans-acting Csm6 RNase.

177 that Piezo1 in ECs senses single-strand RNA (ssRNA) from intestinal microbiota to promote serotonin p

178 ecifically recognizes the single strand RNA (ssRNA) of viral or nonviral origin.

179 ncing results in enhanced single-strand RNA (ssRNA) replication of RSVand Sendai virus, due to decrea

180 modifications to optimize single-strand RNA (ssRNA)-mediated mRNA knockdown.

181 e-strand RNA (dsRNA) over single-strand RNA (ssRNA).

182 cognition of dsRNA over single-stranded RNA (ssRNA) and dsDNA regions at near-physiological condition

183 c reconstruction of the single-stranded RNA (ssRNA) content in one of the three otherwise identical v

184 HCV double-stranded to single-stranded RNA (ssRNA) correlated positively with ISG induction.

185 a mosquito-transmitted single-stranded RNA (ssRNA) flavivirus, causes human disease of variable seve

186 l structures containing single-stranded RNA (ssRNA) free of strong base pairing interactions can be c

187 RVFV has a trisegmented single-stranded RNA (ssRNA) genome.

188 mbusviridae family have single-stranded RNA (ssRNA) genomes with T=3 icosahedral protein shells with

189 n assay between SLA and single-stranded RNA (ssRNA) indicate that SLA competes with ssRNA for the sam

190 hages, particularly the single-stranded RNA (ssRNA) leviviruses, have a previously unappreciated capa

191 ence pathways use small single-stranded RNA (ssRNA) molecules that guide proteins of the Argonaute (A

192 arious short 20-24 base single-stranded RNA (ssRNA) oligonucleotides from a target solution.

193 Poly(I:C), but not single-stranded RNA (ssRNA) or a standard DC maturation cocktail, elicited ty

194 y of the positive-sense single-stranded RNA (ssRNA) Orsay virus (OV) as a natural pathogen of the nem

195 d sequentially as three single-stranded RNA (ssRNA) segments into an icosahedral procapsid which serv

196 sociation constant to a single-stranded RNA (ssRNA) sequence adjacent to the branch site and can bloc

197 are the active sites of single-stranded RNA (ssRNA) synthesis; (v) at late times postinfection, only

198 s with high affinity to single-stranded RNA (ssRNA) targets matching the Cas9-associated guide RNA se

199 ayer particles, nascent single-stranded RNA (ssRNA) transcripts (termed in vitro ssRNA) were found to

200 ay have occurred from a single-stranded RNA (ssRNA) virus (hypovirus) to a dsRNA virus, SsMBV1.

201 respond to unclassified single-stranded RNA (ssRNA) viruses and viruses belonging to the Rhabdovirida

202 effective antiviral for single-stranded RNA (ssRNA) viruses because it programmably cleaves RNAs comp

203 which resembles that of single-stranded RNA (ssRNA) viruses but differs from the well-established mec

204 Single-stranded RNA (ssRNA) viruses form a major class that includes importan

205 DC responses to certain single-stranded RNA (ssRNA) viruses occur only after live viral infection.

206 While most T=3 single-stranded RNA (ssRNA) viruses package in vivo about 3,000 nucleotides (

207 nto a preformed capsid, single-stranded RNA (ssRNA) viruses, such as bacteriophage MS2, co-assemble t

208 Single-stranded RNA (ssRNA) viruses, which include major human pathogens, pac

209 e-stranded DNA (dsDNA), single-stranded RNA (ssRNA), and ssDNA/reverse-transcribing viruses could be

210 and uridine-rich viral single-stranded RNA (ssRNA), including influenza virus ssRNA.

211 o2) and Cryptosporidium single-stranded RNA (ssRNA), we induced specific slicing in Cryptosporidium R

212 nt from the upper stem-single-stranded RNA (ssRNA, terminal loop) junction or ~11 nt from the lower

213 e with positive-sense, single-stranded RNA (+ssRNA) genomes, are abundant in tropical and temperate c

214 ibiting positive-sense single-stranded RNA (+ssRNA) viral infection, especially since RNA is not know

215 nome of negative-sense single-stranded RNA (-ssRNA).

216 in the positive-sense, single-stranded RNA [ssRNA(+)] arteriviruses.

217 -borne, negative-sense, single-stranded RNA [ssRNA(-)] nairovirus that produces fever, prostration, a

218 enic positive-sense, single-stranded RNA [(+)ssRNA] virus families which carry a macro domain: Corona

219 on of positive-sense single-stranded RNA [(+)ssRNA] viruses requires the immediate translation of the

220 ereas negative-sense single-stranded RNA [(-)ssRNA] viruses carry at infection an RNA replicase that

221 oducts (siRNA) and short single-strand RNAs (ssRNA).

222 Single-stranded RNAs (ssRNAs) are ubiquitous RNA elements that serve diverse f

223 s upon the addition of single-stranded RNAs (ssRNAs) identified a group of residues that form a posit

224 hese highly structured single stranded RNAs (ssRNAs) with emphasis on their presence and function in

225 e 11 group A RV segment-specific (+)RNAs [(+)ssRNAs], a chimeric plasmid was transfected, from which

226 60 and an IC generated by incubation of Ro60-ssRNA with an IgG fraction from a CHB mother or affinity

227 dent single-stranded RNA bacteriophages (RPD ssRNA phages), C-1 and Hagl1.

228 escent reporter strain of the negative-sense ssRNA vesicular stomatitis virus (VSV), we show that mic

229 Its positive-sense ssRNA genome of 3,569 bases is enclosed in a capsid with

230 , we have used four strand-specific RNA-seq (ssRNA-seq) datasets collected under two experimental con

231 e strand-specific paired-end RNA sequencing (ssRNA-seq) data from 376 cancer samples covering nine ti

232 scription is strand-specific RNA sequencing (ssRNA-seq).

233 Subsequently, the medium to larger size ssRNAs are recruited until the complete genome is packag

234 Although the F-specific ssRNA phage MS2 has long had paradigm status, little is

235 of genome-capsid interactions in a spherical ssRNA virus provides insight into genome delivery via th

236 f the crRNA with different lengths of ssDNA, ssRNA, and phosphorothioate ssDNA, we discover a self-ca

237 loop) junction or ~11 nt from the lower stem-ssRNA junction to determine the cleavage site.

238 distances from both the lower and upper stem-ssRNA junctions to determine the cleavage site in human

239 atory syncytial virus (RSV), negative strand ssRNA virus, depends upon the ability to recognize speci

240 , some having dsRNA and some positive-strand ssRNA genomes.

241 termediate between dsRNA and positive-strand ssRNA viruses, as well as between encapsidated and capsi

242 avage sites were not observed which suggests ssRNA may operate through a mechanism beyond conventiona

243 Cs by polyuridylic acid (polyU), a synthetic ssRNA analog, generates a strong specific cytotoxic resp

244 , there are no general methods for targeting ssRNA with designed proteins.

245 nded DNA with three-fold lower affinity than ssRNA of the same length and sequence, while binding to

246 ffect of Slfn11 on protein synthesis than (-)ssRNA viruses.

247 We discovered that ssRNA phage infection triggers the release of F-pili fro

248 Prior studies have shown that ssRNA binds to NPH I, but it does not activate ATPase ac

249 We report in this study that ssRNA causes neurodegeneration and neuroinflammation dep

250 e incoming viral genome, we reasoned that (+)ssRNA viruses will be more sensitive to the effect of Sl

251 The ssRNA-modified SiNPs are collected from the target solut

252 The ssRNA-TNP is formed by the complementary association of

253 ustrate a fundamental difference between the ssRNA organization in the multipartite BMV viral capsid

254 few phage genomes are available to date, the ssRNA Leviviridae are a rich source of novel Sgls, which

255 However, the ssRNA contained within HBcAg(183) does function as a TLR

256 oncentrated prior to detection; and (iv) the ssRNA-modified SiNPs give an enhanced SPRI signal upon h

257 f this process, we fluorescently labeled the ssRNA phage MS2 to track F-pilus dynamics during infecti

258 RNA-protein fingerprinting assay, to map the ssRNA binding sites of recombinant VP6 and the genomic d

259 l proteomic approach was utilized to map the ssRNA/dsRNA binding sites of a purified recombinant prot

260 Here we present structures of the ssRNA phage MS2 in complex with the Escherichia coli F-p

261 the SiNPs enhance the diffusion rate of the ssRNA to the surface; (iii) the SiNPs can be collected,

262 sDNA that is complementary to one end of the ssRNA transcript.

263 e Belt and Wedge regions that clamp over the ssRNA.

264 enomic size differences, it appears that the ssRNA binding (R) domain of the capsid diverged evolutio

265 This binding site is similar to the ssRNA-binding site of the sterile alpha motif domain of

266 We propose a multistep model in which the ssRNA phage binds to the F-pilus and through pilus retra

267 The Kd values for the ssRNAs ranged from 3- to 40-fold weaker than the Kd for

268 sition of the 3'-terminal nucleotides of the ssRNAs exhibited the greatest effect on Dicer binding.

269 e dsDNA with an affinity comparable with the ssRNAs affinity, indicating that the binding site has an

270 nificant effect on the replication of the (-)ssRNA viruses vesicular stomatitis virus (VSV) (Rhabdovi

271 This ssRNA-TNP has a simpler structure, better stability, and

272 addition, YdbC binds with lower affinity to ssRNA, making it a versatile nucleic acid-binding domain

273 site effect with ~1.5-2x stronger binding to ssRNA containing N6mA than to the corresponding DNA.

274 Because TLR7(rsq1) binds to ssRNA, our studies imply that the N-terminal portion of

275 the host sex pilus and mechanisms underlying ssRNA-capsid co-assembly, and inspires speculation about

276 To identify cis-NATs using ssRNA-seq, we developed a new computational method based

277 After recognition of a viral ssRNA genome, Nod2 used the adaptor protein MAVS to acti

278 matory proteins upon bacterial LPS and viral ssRNA stimulation was higher in CM and NCM of the Ob gro

279 g antiviral effects when stimulated by viral ssRNA.

280 evidence that synonymous mutations in viral ssRNA genomes are not strictly neutral.

281 TLR8 recognize specific intracellular viral ssRNA sequences, but in human alveolar macrophages, thei

282 yD88-mediated events, as do sensing of viral ssRNA and the drug imiquimod.

283 ly synthetic TLR7/8 ligand, a mimic of viral ssRNA, induced IL-23 production by LP CD1c+ DCs, and thi

284 n dNTP pool levels and the presence of viral ssRNA.

285 TLR7 and TLR8 and mouse TLR7 recognize viral ssRNA motifs and induce antiviral immunity.

286 e receptors (TLR) 7 and 9, which sense viral ssRNA and CpG DNA, respectively.

287 length, we predict the R(g) values of viral ssRNAs are smaller than those of nonviral sequences.

288 The MLD values of viral ssRNAs that package into capsids of fixed size are shown

289 encoded non-structural protein NSP2 to viral ssRNAs results in the remodeling of RNA, which is conduc

290 anded RNA (ssRNA), including influenza virus ssRNA.

291 Enzymatic studies demonstrated that in vitro ssRNA and in vivo 6 hr large RNA samples contain uncappe

292 to innate immune signaling by both in vitro ssRNA and in vivo 6 hr large RNAs.

293 ded RNA (ssRNA) transcripts (termed in vitro ssRNA) were found to be potent IFN inducers.

294 of newly generated nucleotide overhangs when ssRNA is targeted by a designed complementary guide sequ

295 l behavior is limited to structures in which ssRNA directly engages in tertiary interactions or is re

296 RNA (ssRNA) indicate that SLA competes with ssRNA for the same binding site on the NS5 polymerase.

297 ing domain of Lassa virus NP in complex with ssRNA.

298 Supernatants generated from macrophages with ssRNA in the presence of IRS661 or chloroquine did not c

299 n levels by transfecting live parasites with ssRNA-hAgo2 complexes.

300 generated from macrophages transfected with ssRNA or incubated with the IC.