ライフサイエンスコーパス: stable cell line

1 ng of PML target genes using a PML-inducible stable cell line.

2 chia pastoris), COS-1 cells and in an HEK293 stable cell line.

3 cation of HpSlyD and in an HpSlyD-expressing stable cell line.

4 s, E1 functions are supplied in trans from a stable cell line.

5 recordings from human Na(v)1.7 channels in a stable cell line.

6 spectroscopy, the purified receptor, and the stable cell line.

7 fluorescent protein-tagged proteins in human stable cell lines.

8 nd constitutive expression in microsatellite-stable cell lines.

9 ector was used to create geneticin-resistant stable cell lines.

10 tically accelerate the production of complex stable cell lines.

11 rearrangements of the actin cytoskeleton in stable cell lines.

12 se (ERK) 2 activation compared with beta(1A) stable cell lines.

13 he host cells, thus leading to generation of stable cell lines.

14 n transient transfection systems, but not in stable cell lines.

15 elevance to experiments requiring the use of stable cell lines.

16 n of GP Ib alpha was equivalent in these two stable cell lines.

17 re expressed both in insect cells and in CHO stable cell lines.

18 he neo gene and selected in G418 to generate stable cell lines.

19 es can be isolated and expanded to establish stable cell lines.

20 ion of IRF3, which was limited in E1A and E7 stable cell lines.

21 atic aneuploidy in previously karyotypically stable cell lines.

22 nt human embryos, primordial germ cells, and stable cell lines.

23 tide helical structure and generated several stable cell lines.

24 umors were transplantable and established as stable cell lines.

25 Here, we present an inducible GFP-NONO stable cell line and use it for live-cell 3D-structured

26 oth Trps1-deficient and Trps1-overexpressing stable cell lines and analyzed the progression of minera

27 the human CRF1 and CRF2 receptor subtypes in stable cell lines and characterized 125I-Tyr0-sauvagine,

28 how that epiblast cells can be maintained as stable cell lines and interrogated to understand how plu

29 d with elevated translation levels in pooled stable cell lines and isolated single-cell clones.

30 rus-associated APO3G and (ii) we constructed stable cell lines and selected clones expressing compara

31 n fluorescence protein (GFP) expressing RGC5 stable cell lines and studied the changes in cell migrat

32 oid cell type-specific expression of PU.1 in stable cell lines and transgenic animals is conferred by

33 Stable cell lines and transgenic mice that express G100L

34 fragment for expression of reporter genes in stable cell lines and transgenic mice.

35 Using both stable cell lines and transient transfection we demonstr

36 Here we show, using stable cell lines and transient transfections, that over

37 tion mutant lacking only this alpha-helix in stable cell lines and Xenopus laevis photoreceptors.

38 xpression of FGE, either transiently or as a stable cell line, and the resulting aldehyde can be sele

39 o estimate receptor expression level for the stable cell line, and titration of a membrane preparatio

40 ed the mutant apoA-I forms to establish nine stable cell lines, and developed strategies for the larg

41 elevant systems, including purified enzymes, stable cell lines, and virally infected cells.

42 e DAT within plasma membrane microdomains in stable cell lines, and was essential for amphetamine-ind

43 Using a stable cell line approach, we characterize the activatio

44 Using both transient transfection and stable cell line approaches as well as soft agar assay,

45 teomic and single-cell genomic approaches in stable cell lines as well as induced pluripotent stem ce

46 protein cargo, by transient transfection or stable cell line-based production.

47 Toward this end, we established stable cell lines by introducing a dominant-negative mut

48 xin-inducible degradation system, we created stable cell lines capable of ablating ORC2 rapidly, reve

49 p53-mediated tumor suppression, we generated stable cell lines capable of expressing exogenous EphA2

50 We have established several stable cell lines capable of inducibly expressing a p53

51 Stable cell lines (CHO) transfected with either human co

52 Stable cell lines constitutively expressing Rd2 were est

53 with the ARMMs loading construct or using a stable cell line containing a transgene that encodes the

54 and 3 were transfected into Huh-7 cells, and stable cell lines containing functional replicons were s

55 Experiments with stable cell lines created by transfection with either wi

56 3(280)A, and W6.48(357)A mutant receptors in stable cell lines created in HEK cells for agonist-stimu

57 ing bacterial artificial chromosome-minigene stable cell lines, CRISPR/Cas9 enhancer-deleted daughter

58 assays; moreover, overexpression of p202a in stable cell lines decreased the endogenous levels of mRN

59 In addition, these stable cell lines demonstrated an increased proliferatio

60 knockdown of Drosophila CTCF by RNAi in our stable cell lines demonstrates that CTCF itself is criti

61 Employing a stable cell line derived from p53-deficient human fibrob

62 rated nonnative amino acids were produced in stable cell lines derived from a CHO cell line with tite

63 e of Brn-3b in breast cancer, we established stable cell lines derived from the MCF7 human breast can

64 eractions, we separately introduced into the stable cell line either D3 receptors carrying an hemaggl

65 after DNA transfer and titers obtained from stable cell lines, emerging weeks later, showed strong c

66 ineered the genome of influenza A viruses in stable cell lines engineered for virus production to int

67 imilar patterns, but gp120 purified from the stable cell line exhibited higher homogeneity.

68 Mutant stable cell lines exhibited a stronger inhibition of IFN

69 Stable cell lines expressed ribozymes at modest levels (

70 cellular processes, we describe the use of a stable cell line expressing a fusion of green fluorescen

71 a red fluorescent VP26 fusion (PRV180) on a stable cell line expressing a green VP26 fusion (PK15-mN

72 We developed a stable cell line expressing alpha-SYN endogenously tagge

73 By using a stable cell line expressing dominant negative Smad3, we

74 irus, generated separately by infection of a stable cell line expressing E2-G or E2DeltaHVR1-G with a

75 A stable cell line expressing EB1-green fluorescent protei

76 er demonstrate that extracts prepared from a stable cell line expressing epitope-tagged wild-type TBP

77 We have generated a stable cell line expressing FLAG epitope-tagged D3 dopam

78 We created a stable cell line expressing FoxI1-green fluorescent prot

79 facilitate the VLP platform, we generated a stable cell line expressing high levels of ZIKV prM-E pr

80 human RPE65 using the adenovirus system in a stable cell line expressing lecithin retinal acyltransfe

81 We prepared a stable cell line expressing the glucagon receptor to cha

82 A stable cell line expressing the most effective intrabody

83 It takes ~3 weeks to establish a stable cell line expressing the mutant enzyme and 5 more

84 c transposons generated a heterozygous SCN1A stable cell line expressing two separate alleles of the

85 ant Ras gene (Ras-ala15) in a Drosophila S-2 stable cell line expressing X (X-S2), or incubation of t

86 fers an excellent opportunity for generating stable cell lines expressing a defined single molecule p

87 isolation of a GPN1/GPN3/RNAPII complex from stable cell lines expressing a dominant negative GPN1 ha

88 to activate gene expression, we constructed stable cell lines expressing a splicing reporter based o

89 Stable cell lines expressing a WT, or mutants in which t

90 We created stable cell lines expressing ACII and the D2L receptor,

91 Stable cell lines expressing an activated mutant of mTOR

92 ibits tumor promoter-induced transformation, stable cell lines expressing antisense Pdcd4 were genera

93 Using stable cell lines expressing both OB-Ra, the most abunda

94 raminidase-deficient HPF3 variant (C28a) and stable cell lines expressing C28a or wild-type (wt) HN.

95 Stable cell lines expressing canine CTLA4 and FcyRI were

96 We established several stable cell lines expressing CD163 cDNAs from pig, human

97 binding sites in RNA genome packaging using stable cell lines expressing competing wild-type and mut

98 rized and polarized conditions, we developed stable cell lines expressing deltaF508 or wild-type CFTR

99 Using stable cell lines expressing E2 we show that this downre

100 acterized and their binding capacity towards stable cell lines expressing EGFRvIII, EGFR wild type (E

101 EC50 and gamma(noise) values for SYM 2081 in stable cell lines expressing either (GluR6(R) or GluR6(R

102 Stable cell lines expressing either full-length or trunc

103 d EC50 and gammanoise values for SYM 2081 in stable cell lines expressing either GluR6(R) or GluR6(R)

104 ration and survival in Chinese hamster ovary stable cell lines expressing either human beta(1C) or hu

105 Here, we employ a panel of Drosophila S2 stable cell lines expressing epitope-tagged exosome subu

106 To this end, stable cell lines expressing fatty acid transporters as

107 Here, we constructed stable cell lines expressing fluorescent-tagged wildtype

108 Stable cell lines expressing green fluorescent protein (

109 release of alkaline phosphatase (AP) in THCE stable cell lines expressing HB-EGF-AP.

110 By constructing stable cell lines expressing JCV T125A and T125D mutants

111 ivate the pro-survival protein kinase Akt in stable cell lines expressing K721M and ErbB2 but, unlike

112 Stable cell lines expressing mSTRPC4 showed abundant mST

113 ion is the strategy of choice for generating stable cell lines expressing multiple genes for the stud

114 he strategy of these studies was to generate stable cell lines expressing murine AHR proteins that we

115 dvantage in human cancer cells, we generated stable cell lines expressing p53 mutants p53-R175H, -R27

116 lymphoid cell line, BaF3, for generation of stable cell lines expressing PAR-2 (BaF3/PAR-2) or the n

117 P2E1 in ethanol-mediated oxidative stress in stable cell lines expressing predominantly mt CYP2E1 or

118 the widespread availability of plasmids and stable cell lines expressing proteins fused to GFP.

119 ceptors were transfected into 293 cells, and stable cell lines expressing similar numbers of receptor

120 on (STAT) signaling, HeLa- and MCF-7-derived stable cell lines expressing SOCS1, SOCS2, and SOCS3 pro

121 Stable cell lines expressing the mutant polypeptide secr

122 + channel permeation pathway, we created two stable cell lines expressing the voltage-dependent rat s

123 binding of specific CC or CXC chemokines to stable cell lines expressing their respective high affin

124 Stable cell lines expressing these mutations were assess

125 With stable cell lines expressing these ORFs, we analyzed the

126 Surprisingly, stable cell lines expressing VEGF-A siRNAs silenced VEGF

127 ransfected with HDV cDNA do not give rise to stable cell lines expressing viral antigens or replicati

128 ed for pharmacologic chaperoning activity in stable cell lines expressing wild-type and P23H opsin.

129 -mediated gene transfer was used to generate stable cell lines expressing wild-type CD1d or a chimeri

130 e-cycle LASV replicon particles (VRPs) and a stable cell line for their production.

131 s of elevating the Gab2 level in K562 cells, stable cell lines for doxycycline-inducible expression o

132 echanisms and for considering development of stable cell lines for liver-directed therapies.

133 bles facile development of viral vectors and stable cell-lines for ncAA mutagenesis.

134 C stabilizers), we generated a CLDN5-P2A-GFP stable cell line from human pluripotent stem cells (hPSC

135 Moreover, we found that a primary stable cell line from wild-type mouse marrow cells expre

136 First, we generated several stable cell lines from melanomas of transgenic mitfa-BRA

137 A stable cell line (GT2-LPk) derived from LLC-Pk was creat

138 type 5-HT2C receptors in an S138R-expressing stable cell line had no effect on ligand binding to wild

139 h content imaging assay that is based upon a stable cell line harboring a visible multicopy ER respon

140 We found that stable cell lines harboring D32A-mutated beta-catenin we

141 ted up to date were unsuccessful in deriving stable cell lines harboring non-cytopathic replicons.

142 Using stable cell lines, harboring wild-type or null-p53, in w

143 A stable cell line has been produced that incorporates hex

144 Tetracycline-inducible HEK293S stable cell lines have been prepared that express high l

145 alls, subcellular organelles and the lack of stable cell lines have prevented routine application of

146 Investigations with both platelets and stable cells lines have shown the PIA2 polymorphism is p

147 We developed a stable cell line, HeLa/CL3-4, expressing MyD88/Bla(a) an

148 Ala94, A1 AR immunoreactivity was present on stable cell lines; however, functional binding sites cou

149 ransgenic mouse model and different microRNA stable cell lines identified Arrdc3, Neurod4, and caspas

150 We could thus create stable cell lines in hepatoma-derived SK-Hep-1 cells tha

151 activation was strongly reduced in beta(1C) stable cell lines in response to fibronectin adhesion an

152 Each of the stable cell lines in which kinase-defective mutants of c

153 titer of the VSV pseudotype, derived from a stable cell line incorporating both of the chimeric glyc

154 To study DBCCR1 function, stable cell lines, inducible for DBCCR1 expression by te

155 of phosphorylation, we generated a panel of stable cell lines inducibly over-expressing PABPN1 with

156 The production of stable cell lines is an important technique in cell biol

157 role in interferon signaling by generating a stable cell line, L-C6, by using the lentiviral expressi

158 This stable cell line (LFBK-alphaVbeta6) showed beta6 express

159 maining low in glioblastoma multiforme (GBM) stable cell lines, low-grade glioma-derived primary cult

160 r improvement including the establishment of stable cell line must be achieved.

161 Similar experiments with a more genetically stable cell line of 184A1 also revealed an increased fre

162 A stable cell line of HEp-2 overexpressing RNase L inhibit

163 cDNA library was generated from A6 cells, a stable cell line of renal distal nephron origin, and the

164 Using stable cell lines of C3H10T1/2 murine fibroblasts that c

165 tivation on BCSG1 expression, we established stable cell lines of T47D that express the dominant nega

166 e biological effect of AS3 was tested in two stable cell lines, one expressing sense and another expr

167 expression of a tagged plasmid, establishing stable cell lines, or laborious purification protocols t

168 Here, we generated a stable cell line overexpressing a dominant-negative poin

169 Employing a stable cell line overexpressing both the human telomeras

170 ion in activated macrophages by generating a stable cell line overexpressing stathmin-GFP.

171 xamination of mitochondrial bioenergetics in stable cell lines overexpressing GFP-tagged p55 variants

172 ATP-induced responses were measured in stable cell lines overexpressing human P2X4 using a YOPR

173 l proliferation arrest was also inhibited in stable cell lines overexpressing MAN1.

174 We show that stable cell lines overexpressing MEKK3 not only had elev

175 siently transfected cells and in transfected stable cell lines overexpressing p202.

176 Here, stable cell lines overexpressing processed forms of SUMO

177 nase (IKK), and IKK activity is augmented in stable cell lines overexpressing TRE17, in a USP-depende

178 Using both stable cell lines overexpressing uPAR and transient gene

179 Stable cell lines overexpressing variable levels of MTF1

180 In the present study, we generated a stable cell line, overexpressing the two types of activi

181 MCF7/PIGR and MDA-MB468/PIGR stable cell lines, overexpressing the PIGR gene, were ge

182 In rat hepatoma, stable cell lines, overexpression of APOBEC-1 resulted i

183 ion of the integrins' biological activities, stable cell lines producing the soluble integrins were g

184 bility to rapidly induce OGT expression in a stable cell line provides an excellent model system to s

185 iety of applications including generation of stable cell lines, recombinant protein production, creat

186 ar expression of this antibody into either a stable cell line replicating subgenomic RNA, or a transi

187 aive Huh7.5 cells with HCV released from the stable cell lines resulted in high levels of HCV protein

188 ene expression profiling of miRNA-expressing stable cell lines revealed 66 genes that were commonly d

189 Analyses of stable cell lines revealed that the carboxy-terminal dom

190 Stable cell lines robustly produce HCV virions with up t

191 K562 transfections generated stable cell lines running for 28 weeks with and without

192 Our stable cell lines secreting individual VLPs provide a fl

193 The 293 T stable cell lines secreting VLPs were adapted to grow in

194 n rhodopsin expressed by the HEK293S GnTI(-) stable cell line showed it to be Man(5)GlcNAc(2).

195 In comparison with the control cells, the stable cell line showed significant Galpha-mediated liga

196 Saturation analysis of a human M1 mAChR stable cell line showed that [(3)H]PT-1284 bound to M1 m

197 LANA expression in these stable cell lines showed a dramatic increase in chromoso

198 By the same strategy, another stable cell line simultaneously overexpressing human 11b

199 A stable cell line simultaneously overexpressing recombina

200 Stable cell line studies determined that transmembrane 2

201 ing an immortalized mouse hepatocyte-derived stable cell line supporting a high level of HBV replicat

202 f a dominant-negative karyopherin-beta1 in a stable cell line supporting HBV replication resulted in

203 n a Drosophila melanogaster Schneider 2 (S2) stable cell line system.

204 cells transfected with Rluc/NeoRep yielded a stable cell line that contains persistently replicating

205 (iii) Passage of UL34-null virus on a stable cell line that expresses CL04 resulted in selecti

206 , because a similar effect was observed in a stable cell line that expresses one-tenth of CFTR.

207 A stable cell line that overexpresses PRAJA exhibits low l

208 AF-1 in cultured cells failed to produce any stable cell line that overexpresses SAF-1.

209 icons, targeting of Sindbis virus replicons, stable cell lines that can be induced to produce replico

210 ant and mutant HIV-1 strains in infection of stable cell lines that coexpress the CD4 and chemokine r

211 /GFP via genetic trans complementation using stable cell lines that constitutively express LCMV or LA

212 candidate to target these diseases by using stable cell lines that continuously secrete VLPs in the

213 a retrovirus-based system, we created HL-60 stable cell lines that express a short-hairpin RNA targe

214 We developed human neuroblastoma stable cell lines that express either wild-type (WT) or

215 of Bin1 during differentiation, we generated stable cell lines that express exogenous human Bin1 cDNA

216 components of the SMN complex, we generated stable cell lines that express FLAG-tagged SMN or Gemin2

217 ruited to genomic binding sites, we analyzed stable cell lines that express tagged wild-type and muta

218 31 breast cancer cells were used to generate stable cell lines that express WT-ER, Y537S, or Y537C mu

219 grin inside-out signaling, we generated K562 stable cell lines that expressed LFA-1 (alpha(L)beta(2))

220 pressing tumorigenesis by establishing BT549 stable cell lines that expresses full-length BAF57 prote

221 By using stable cell lines that inducibly express CIITA or variou

222 Here, we report the creation of stable cell lines that inducibly produce non-infectious

223 Stable cell lines that overexpress HDAC3 show a decrease

224 ne (DEX) or ethanol (EtOH) and by generating stable cell lines that overexpressed either wild type (W

225 Stable cell lines that produce the RRV glycoprotein-pseu

226 le of PTP-PEST using Rat1 fibroblast-derived stable cell lines that we have engineered to overexpress

227 We isolated stable cell lines that were resistant to OHT-induced apo

228 For flexibility in generating stable cell lines the sgRNAs have been cloned in a lenti

229 r, we also found that in the knockout-TXNDC5 stable cell lines, the HEV ORF3 protein may hijack other

230 Here, we use a chromosomally stable cell line to test the effect of BRG1 loss on the

231 Here, we used stable cell lines to demonstrate the SMN C-terminus modu

232 Stable cell lines transfected with this vector express t

233 response element (PPRE)-reporter assays in a stable cell line treated with GA resulted in enhanced ex

234 NonRER (microsatellite stable) cell lines typically displayed highly aberrant k

235 proliferation, morphology, and cyclin D1 in stable cell lines unmasked an unexpected growth promotin

236 utions on SIM1 transcriptional activities in stable cell lines using luciferase gene reporter assays.

237 P50-mutant viruses in an APOBEC3-expressing stable cell line was also much slower than that of WT vi

238 mechanisms that regulate HB-EGF shedding, a stable cell line was established expressing HB-EGF(TM) i

239 strated using transient transfections, and a stable cell line was selected.

240 sVCAM-1 binding to homogenous stable cell lines was also cell-type specific, and requi

241 Inhibition of cell proliferation in beta(1C) stable cell lines was attributable to an inhibitory effe

242 Using stable cell lines, we show that artificial tandem arrays

243 Stable cell lines were created in which either IRP1, IRP

244 Stable cell lines were created that express cDNAs for th

245 Stable cell lines were engineered to express adipogenic

246 natural host, embryonic chick chondrocytes; stable cell lines were established as well.

247 Stable cell lines were established by transfecting 293-E

248 the LAT-deficient Jurkat derivative, J.CaM2, stable cell lines were established expressing various ty

249 oying expression plasmids for TM-2 and TM-3, stable cell lines were established from the NRK 1569 cel

250 y Western blot, and activity measurement and stable cell lines were established.

251 Chinese hamster ovary cells (745 cells), and stable cell lines were established.

252 Stable cell lines were generated and tested for their ab

253 Stable cell lines were generated expressing this recepto

254 sense orientation and with empty vector, and stable cell lines were generated.

255 After transfection, stable cell lines were isolated that expressed ZAP70, Sy

256 Stable cell lines were isolated that gave tetracycline/s

257 the C-terminal zinc finger-like domain, and stable cell lines were isolated.

258 In this study stable cell lines were made that express antisense RCP R

259 iously to support the HPV-16 life cycle, and stable cell lines were obtained that harbored the E7-def

260 Three stable cell lines were shown by western blot analysis to

261 These stable cell lines were susceptible to PRRSV infection an

262 Furthermore, all three stable cell lines were tumorigenic when injected into nu

263 effects of 53BP2, we have constructed HEK293 stable cell lines where 53BP2 expression can be regulate

264 levels of ELF in comparison to a Delta-PRAJA stable cell line, where ELF expression is high compared

265 activity of the DeltaN variant, we generated stable cell lines, which inducibly express DeltaNp73alph

266 significance of 53BP2 induction, we utilized stable cell lines with a ponasterone A-regulated 53BP2 c

267 Stable cell lines with comparable expression of wild typ

268 Stable cell lines with controlled increase in Anxa6 leve

269 tes in the apoptotic pathway, we constructed stable cell lines with different CSB domain disruptions.

270 s in the human cancer cells studied--even in stable cell lines with diminished expression of SRP comp

271 ting mutation Galpha(q)(Q209L) and developed stable cell lines with doxycycline-inducible RGS2 protei

272 We have developed a variety of stable cell lines with genetic modification of mTor acti

273 Metaphase spreads derived from stable cell lines with inducible CBX fusion expression r

274 , cells incubated with MIR21 inhibitors, and stable cell lines with inducible expression of MIR21.

275 ng of EcNHX1, EcNHX3, and EcNHX4 resulted in stable cell lines with largely diminished capacities of

276 mic analysis of lipid-rafts derived from PC3 stable cell lines with overexpression or knockdown of CX

277 ocesses, we used RNA interference to created stable cell lines with reduced HMGN1 protein levels and

278 Lastly, using stable cell lines with suppressed TFII-I levels, we show

279 e developed a simple approach for generating stable cell lines with variable copy numbers of any BAC.