ライフサイエンスコーパス: stepping

1 ents, spontaneous kicking and weight-bearing stepping.

2 e motor head binds to the microtubule during stepping.

3 fundamentally from that of plus-end-directed stepping.

4 minus end-directed to net plus end-directed stepping.

5 from minus end-directed to plus end-directed stepping.

6 eps to detachments without affecting forward stepping.

7 numerical modeling of myosin V diffusion and stepping.

8 xplain the accelerated triggering of induced stepping.

9 al ladder, a complex task requiring accurate stepping.

10 their involvement in control of accuracy of stepping.

11 I4 depolarization never elicited stepping.

12 es of inability to produce effective forward stepping.

13 y, processivity, and probability of backward stepping.

14 across different cycle durations of neonate stepping.

15 lly limited by the need for mechanical phase stepping.

16 slow step for KIF3C resulting in asymmetric stepping.

17 ilateral limb pairs move out-of-phase during stepping.

18 es locomotor speed and ultimately terminates stepping.

19 y modulation recorded in this nucleus during stepping.

20 he STN is involved in the dynamic control of stepping.

21 onformation of dynein optimal for processive stepping.

22 e the effectiveness of random, uncoordinated stepping: (1) the formation of three-headed and four-hea

23 spends 65% of its time undergoing processive stepping, 4% undergoing 1D diffusion, and the remaining

24 For voluntary stepping, a disruption in the normal coupling between po

25 echanism and coordination of KIF3A and KIF3B stepping, a presteady-state kinetic analysis was pursued

26 s showed significant improvement in hindlimb stepping ability, quadrupedal weight support, and all me

27 During induced stepping, accelerated step initiation may represent an e

28 We propose that the minus end-directed stepping action of Cut7 is selectively inhibited by coll

29 r neighbors inhibit their minus end-directed stepping action, but not their plus end-directed steppin

30 ping action, but not their plus end-directed stepping action.

31 Finally, SNC-80 more potently stimulated stepping activity in mice lacking the NOP receptor than

32 ty relative to KIF3AA yet is more capable of stepping against mechanical load than KIF3CC.

33 ental data from GFP-labeled kinesin-1 motors stepping along immobilized microtubules, we show 1) that

34 kinesin-1 and kinesin-2 neck-linker chimeras stepping along microtubules in the absence or presence o

35 mer 'gate' each other to promote coordinated stepping along microtubules.

36 e the ability to switch protofilaments while stepping along the microtubule when challenged by an obs

37 ve functions are linked to delayed voluntary stepping, alterations in the coupling of posture and loc

38 ull-length GiKIN14a has significantly higher stepping and ATP hydrolysis rates than does GiKIN14a-Del

39 rface, a task that does not require accurate stepping and can be accomplished without the motor corte

40 o show that Lis1 does not directly alter the stepping and force production of individual dynein motor

41 errors in the parametric control of induced stepping and its effectiveness in stabilizing balance wo

42 We found that the neuromuscular modules of stepping and kicking differ substantially.

43 s dual functionality, with microtubule-based stepping and regulation of microtubule dynamics.

44 he rhythmic output required for coordinating stepping and stabilizing the body axis during movements.

45 he rhythmic output required for coordinating stepping and stabilizing the body axis during movements.

46 remarkably consistent alignment between the stepping and stimulation cycle when the stimulation spee

47 e gating mechanism, the pathway for backward stepping, and the energy consumption as a function of AT

48 A new hybrid time stepping approach, which couples the Runge-Kutta and New

49 number of steps per day and the intensity of stepping are associated with lower mortality.

50 treadmill, with the left and right hindlimbs stepping at different speeds.

51 No signs of processive stepping at the single molecule level were observed in t

52 ed out using a model that incorporated motor stepping, attachment/detachment rates, and intermotor fo

53 , chemical and torsional barriers, sometimes stepping back and cleaving off nucleotides from the grow

54 Pase rate, microtubule-binding affinity, and stepping behavior of dynein, and that microtubule bindin

55 -molecule microscopy to directly observe the stepping behavior of kinesin-1 and -2 family motors with

56 e consistent with a model wherein the normal stepping behavior of the myosin is supplemented by the p

57 standing dynein's directionality and unusual stepping behaviour.

58 the model still shows processive directional stepping, but it becomes uncoordinated and the stepping

59 Here, we examined how base triplet stepping by RecA, Rad51, and Dmc1 is affected by DNA seq

60 at ATP hydrolysis in the bound head precedes stepping by the tethered head, leading to a mechanochemi

61 b CPGs and that the ability of SCA to induce stepping can be enhanced by the sacral CPGs.

62 lts, we propose a quantitative model for the stepping characteristics of dynein and its response to c

63 Since kicking and ground-stepping coexist at birth, switching between the two beh

64 ents as evidence of the STN's involvement in stepping control.

65 elongation rate intrinsic to the ribosome's stepping cycle and the actual elongation rate that takes

66 dates an essential role for AAA4 in dynein's stepping cycle and underscores the complexity and crosst

67 ics of structural transitions underlying the stepping cycle of kinesin-1 at physiological ATP, we use

68 rimental grounds, the repercussions of their stepping cycle on the collective dynamics still remains

69 aturating ATP, kinesin-1 spends half of each stepping cycle with one head bound, specifying a structu

70 the energy requirements for the fundamental stepping cycle, which likely includes energy from glycos

71 is the rate-limiting transition in the KIF1A stepping cycle.

72 the stance and swing phases of the backward stepping cycle.

73 viously undescribed registry during dynein's stepping cycle.

74 that trigger the two key transitions in the stepping cycle: detachment of the trailing head from the

75 cargos against load without coordinating the stepping cycles of its two heads.

76 to individual motor domains in processively stepping dimers.

77 for full-length Cut7, the key determinant of stepping direction is the degree of motor crowding on th

78 yeast kinesin-5 motors, Cut7 can reverse its stepping direction, by mechanisms that are currently unc

79 s presented show that the kinesin processive stepping distance decreases 40-60% with EC50 values <100

80 Institute and Harvard Dental School, and his stepping down as editor of the "Discovery!" section of t

81 Eligibility for stepping down asthma controller medications included no

82 We sought to compare the safety and costs of stepping down asthma controller medications with maintai

83 Limited data exist regarding outcomes after stepping down asthma medication.

84 Stepping down asthma medications in those whose symptoms

85 Guidelines recommend stepping down asthma treatment to the minimum effective

86 Stepping down medication did not adversely affect outcom

87 general asthma population and assess whether stepping down medication increased exacerbations or reli

88 Stepping down medication, either LABAs or ICSs, could sa

89 patients were drawn to assess the impact of stepping down medication: mean age 50.4 years, 39.4% mal

90 rmation.Objectives: Report ACM and impact of stepping down therapy, following collection of additiona

91 95% or more of the Cyt c population folds by stepping down through the same set of foldon-dependent p

92 with the other factors to reduce chances of stepping down, but did not constitute a problem factor w

93 The distribution of stepping durations of KIF3AC molecules is described by a

94 germane to the findings of our Report: that stepping dynamics provided a better statistical descript

95 e-trial analysis of licking behavior reveals stepping dynamics with variable onset times, which is re

96 hesized goals also failed to replicate human stepping dynamics.

97 ing variables, but could not replicate human stepping dynamics.

98 ous diffusion-to-bound dynamics or discrete "stepping" dynamics.

99 tral and peripheral stalks affects c-subunit stepping efficiency.

100 st product dissociation, and that isoprenoid stepping ensures short transit times.

101 was not expressed during swimming, treadmill stepping, exploratory locomotion, or walking on an uncoa

102 In permeabilized cells, stepping external [Ca(2+)] from 0 to 3 mum also produced

103 ons of this analysis include molecular-motor stepping, fluorophore bleaching, electrophysiology, part

104 rvation of enzymatic memory in translocation stepping, for the kinetics of translocation steps of dif

105 ehind the semi-synthetic modification of Hy, stepping forward to cancer chemotherapy.

106 onding to random thermal fluctuations of the stepping head while it searched for its next actin-bindi

107 We tracked the development of ground-stepping in 4- to 48-mo-old infants and found that, afte

108 zed into a hierarchy that precisely controls stepping in a stereotypic, speed-dependent fashion.

109 After a prolonged period stepping in circles, people walk with a curved trajector

110 tivity in the lumbosacral spinal cord during stepping in newborns, toddlers, preschoolers, and adults

111 f the track does not automatically instigate stepping in the absence of an additional stimulus (heat

112 evocation of spontaneous and actively driven stepping, including optimization of these behaviors in d

113 Furthermore, a remarkable feature of newborn stepping is a higher overall activation of lumbar versus

114 During imbalance, stepping is a powerful protective action for preserving

115 Newborn stepping is characterized by an alternating bilateral mo

116 The basic rhythmic activity that underlies stepping is generated by a neural network, situated in t

117 g and falls, initiation slowing of voluntary stepping is observed together with perturbation-induced

118 epresent an event-triggering process whereby stepping is released according to the occurrence of a pe

119 rference (i.e., load-dependent modulation of stepping kinetics and binding-site exclusion), whereas f

120 ase cycle suggesting that within KIF3AC, the stepping kinetics are similar for each head during the p

121 Analysis of stepping kinetics in varying nucleotides shows that ATP

122 To resolve the stepping kinetics of KIF3A and KIF3C motors in homo- and

123 gs are consistent with recent studies of the stepping kinetics of the motor.

124 The randomness of stepping leads to a buildup of tension in the linkage be

125 nt study was designed to further compare the stepping-like movements generated via epidural (ES) and/

126 We examined the ability to generate stepping-like movements in response to ES and/or IS of s

127 relimb movements appeared first, followed by stepping-like movements in the hindlimbs.

128 of the spinal cord at L3-L7 induced hindlimb stepping-like movements on a moving treadmill belt, but

129 t neural mechanisms were activated to induce stepping-like movements.

130 face of the spinal cord) induced quadrupedal stepping-like movements.

131 in rotational mobility were detected during stepping, likely corresponding to random thermal fluctua

132 examine the structural dynamics of dynein's stepping mechanism in real time, we used polarized total

133 her, these observations demonstrate that the stepping mechanism of dynein is fundamentally different

134 many of the critical unknown aspects of the stepping mechanism of myosin 5 including head-head coord

135 ctivity with functional implications for its stepping mechanism, and also reveal a previously unappre

136 In the well-accepted hand-over-hand stepping mechanism, the trailing motor detaches from the

137 ation, in which a translocating RNAP uses a "stepping" mechanism, has been studied extensively, but p

138 f dynein is fundamentally different from the stepping mechanisms of other well-studied MT motors, bec

139 amework that includes all of the alternative stepping mechanisms.

140 n transitions dynamically between the active stepping mode and this weak surface-associated mode enha

141 e window of evidence accumulation, and their stepping model cannot explain existing data as effective

142 accounted for roughly as many neurons as the stepping model.

143 ons we recorded were better described by the stepping model.

144 marine N cycle using a multicompartment time-stepping model.

145 Toward this end, single-molecule, in vitro stepping-motility assays are commonly used to determine

146 for the estimation of motility parameters in stepping-motility assays.

147 ular tension sensing is observed in dynein's stepping motion at high interhead separations.

148 he geometry, we propose a diffusive sideward stepping motion of Kip3 on the microtubule lattice, asym

149 synthase (FOF1) is comprised of two rotary, stepping motors (FO and F1) coupled by an elastic power

150 activity in the STN could entrain patients' stepping movements as evidence of the STN's involvement

151 Human stepping movements emerge in utero and show several mile

152 "control templates" for how humans regulate stepping movements from each step to the next.

153 Thus, the regulation of lateral stepping movements is inherently multi-objective and bal

154 These models regulate stepping movements to achieve any of three hypothesized

155 he numerous possibilities of how to regulate stepping movements to achieve different walking goals.

156 ic pattern when Parkinson's patients perform stepping movements, raising the question whether the STN

157 n humans is causally involved in controlling stepping movements.

158 at the conditional knock-outs used alternate stepping, not hopping, during overground locomotion, sug

159 are consistent with a mechanism in which the stepping of KIF3C can be activated by KIF3A in a strain-

160 In contrast to other processive motors, stepping of the dynein motor domains (heads) is not prec

161 synthase, and explain the need for the finer stepping of the F1 motor in the mammalian system, as see

162 ing of how ATP binding is coupled to forward stepping of the motor.

163 port system to allow bipedal and quadrupedal stepping on a treadmill and in an open field environment

164 vity during more automated behavior, such as stepping on a treadmill.

165 molecular motor myosin V transports cargo by stepping on actin filaments, executing a random diffusiv

166 the walker's center-of-mass will facilitate stepping on target footholds.

167 transfer from simple locomotion to accurate stepping on the ladder slow-conducting PTNs more profoun

168 ransition from simple locomotion to accurate stepping on the ladder, slow-conducting PTNs respond in

169 ses ATP hydrolysis, like other kinesins, for stepping on the microtubule lattice, but at the plus end

170 rmly attached to one microtubule while it is stepping on the other.

171 of those revealed by sporadic weight-bearing stepping, on the other hand.

172 nce, rats avoid a tone-signaled footshock by stepping onto a nearby platform, delaying access to sucr

173 learn to avoid a tone-signaled footshock by stepping onto a nearby platform.

174 y binding their barbed ends and processively stepping onto incoming actin monomers to incorporate the

175 ass platform; forelimb use was unbiased when stepping onto the glass surface but biased when stepping

176 pping onto the glass surface but biased when stepping onto the other side.

177 ions of neurons are well described by either stepping or nonlinear ramping, which may be less categor

178 ike trains are better described by discrete "stepping" or continuous "ramping" dynamics.

179 Stepping out of an aggressively air-conditioned building

180 Hence, studying culture requires stepping out of it.

181 f the PPC of cats walking on a treadmill and stepping over a moving obstacle whose speed of advance w

182 Second, Dmc1 stepping over infrequent mismatches is likely critical f

183 plastic changes that may be adaptive in non-stepping paraplegia after SCI.

184 Alternate stepping pathways also exist, including inchworm-like mo

185 mbers translocate nucleosomes with a similar stepping pattern maintained by the catalytic subunit of

186 structure fully consistent with the variable stepping pattern observed as dynein moves along the MT.

187 The irregular stepping pattern of MyoVc, which may result from flexibi

188 epping, but it becomes uncoordinated and the stepping pattern shows a greater variability, which repr

189 ding and twisting, as well as changes in the stepping pattern, but did not lead to falling.

190 the broad step-size distribution of the MVI stepping pattern.

191 e to the observed spontaneous improvement of stepping performance after incomplete SCI and are at lea

192 speed and spatiotemporal characteristics of stepping persist.

193 el of precision to an otherwise noisy dynein stepping process.

194 ciates at 2.1 s(-1), 20-fold slower than the stepping rate, demonstrating the presence of rear head g

195 ite release rate of KIF1A was similar to the stepping rate, indicating that during stepping, rear-hea

196 d mant-ADP release is 5-fold faster than the stepping rate.

197 to the stepping rate, indicating that during stepping, rear-head detachment is an order of magnitude

198 on below the lesion are thus involved in the stepping recovery after an incomplete SCI.

199 ternating stimulation pattern resembling the stepping-related modulation of activity in the STN could

200 This leaves the accuracy of dynein stepping relatively inaccurate and susceptible to therma

201 ed independently from other core features of stepping, revealing a striking freedom in an otherwise p

202 wever, because sensory feedback entrains the stepping rhythm, it is difficult to reveal central patte

203 rnating DBS (altDBS) could entrain patients' stepping rhythm, suggesting a causal role of the STN in

204 ernating stimulation significantly entrained stepping rhythms.

205 ircling and ameliorated deficits in forelimb stepping similarly to electrical DBS, while optogenetic

206 when the stimulation speed was close to the stepping speed in the five patients that demonstrated si

207 otein from syncytial embryos, which we named Stepping stone (Sstn).

208 symmetric, sparing the basal ganglia, and a stepping stone appearance in the frontal pericallosal re

209 , we show that wood has served not only as a stepping stone between habitats but also as a bridge bet

210 Stepping stone colocalized with Steppke at junctions, an

211 ia intermediate unprotected habitats through stepping stone dispersal.

212 These results provide a stepping stone for engineering more complex nitrogen-ato

213 suggesting that plaque may have served as a stepping stone for environmental microbes to adapt to ho

214 ngst ancient eukaryotes, but also provides a stepping stone for exploration of PTM-reader interaction

215 f molecular recorders, providing a necessary stepping stone for future biological work.

216 power of single-cell RNA-seq and provides a stepping stone for future explorations of cellular heter

217 horseradish and watercress represent a first stepping stone for future whole-genome sequencing effort

218 Hopefully, this review can be used as a stepping stone for the reader wanting to gain a clearer

219 Purified coiled-coil domains of Steppke and Stepping stone heterodimerized through a hydrophobic sur

220 llocation, and this work provides a valuable stepping stone in guiding policy on CSF surveillance and

221 ere that family living acted as an essential stepping stone in the evolution of cooperative breeding

222 We anticipate that our work represents a stepping stone in the understanding and use of recurrent

223 These results represent a first stepping stone into integrated chemical networks regulat

224 erature and argue that these could provide a stepping stone into the identification of scientific bes

225 Thus, coiled-coil heterodimerization with Stepping stone normally recruits Step to junctions.

226 itment at the single cell level, a potential stepping stone to improved lineage manipulation through

227 te solution and concentrated gels, acts as a stepping stone to rationally control the organization in

228 As a stepping stone to this goal, the domain of StarCraft has

229 This work represents a stepping stone toward a better understanding of the WAF

230 8 revealed rewrites in the code base, as the stepping stone toward a Spark implementation.

231 C response to radiotherapy and constitutes a stepping stone toward modeling indirect tumor cell death

232 Importantly, this finding also acts as a stepping stone toward understanding the evolution of emp

233 It is intended to provide a stepping stone towards a wider and more general understa

234 (adding up to 206 nucleotides), an important stepping stone towards RNA self-replication.

235 st that the proposed method can be used as a stepping stone towards the development of an accessible

236 Our work constitutes a stepping stone towards the paradigm of self-correcting q

237 This approach provides a stepping stone towards the rational engineering of molec

238 A useful stepping stone towards this is to construct a mathematic

239 Our work may be used as a stepping stone towards using the HyperCEST technique as

240 A second conserved region of Stepping stone was necessary and largely sufficient for

241 alysis confirms the role of the alkene as a "stepping stone" for electron transfer.

242 Intriguingly, Stepping stone's junctional localization also seems part

243 e-function and other analyses of Steppke and Stepping stone, an implicated adaptor protein.

244 inkage to the junctional targeting region of Stepping stone.

245 al genetic modelling identified two waves of stepping-stone colonization: an eastward wave moving thr

246 This achievement was further employed as a stepping-stone for the characterization of the triflate

247 This study provides a major stepping-stone in the understanding of land plant evolut

248 In stepping-stone models, pairwise F(ST) values between arc

249 ude much of the MPA network that may provide stepping-stone protection for species that must shift th

250 In a first step, we simulated the historical stepping-stone spreading dynamics of 40 marine alien spe

251 es acquisition and allocation, and provide a stepping-stone to developing trait-based approaches for

252 stics, these findings serve as a fundamental stepping-stone to the development of the first large-sca

253 cing greenhouse gas emissions and a possible stepping-stone towards renewable synthetic fuels.

254 erve as an alternative, or even evolutionary stepping-stone, to switching between multiple systems.

255 some of the deleterious mutations served as stepping stones across otherwise impassable fitness vall

256 om land and indicate that islands may act as stepping stones facilitating the transoceanic transport

257 volution, mainly by intermittently providing stepping stones for island colonization.

258 fic and Indian oceans and propose islands as stepping stones for the transoceanic transport of terres

259 points should now be viewed as obstacles or stepping stones is a matter of perspective.

260 Together, these form the stepping stones that are expected to accelerate standard

261 The COVID-19 pandemic has provided the vital stepping stones to improve how clinical trials are condu

262 iption factor-DNA interactions are important stepping stones towards a mechanistic understanding of m

263 opulations are only connected via additional stepping stones was supported by contemporary migration

264 Milu searched for vacant habitat patches as "stepping stones" and established refuge populations.

265 els provide intermediate states that act as "stepping stones" for electrons tunnelling across the dio

266 l, persisting populations, which can act as "stepping stones" that enable a rapid population expansio

267 d animals were subject to chance in finding "stepping stones".

268 ns requires intermediate habitats ("phantom" stepping stones).

269 th increasing charge stabilization on their "stepping stones." The presence of mobile anions facilita

270 , Micronesia, and Melanesia, act as critical stepping-stones for dispersal across the Pacific Ocean.

271 Thus, ribosomal mutations can serve as stepping-stones in an evolutionary path leading to the e

272 als atomically dispersed on oxides are major stepping-stones toward a rational development of single-

273 Using a precision stepping task and synchronizing target visibility to the

274 d that more neurons were better described by stepping than ramping, even when conditioned on evidence

275 Our work reveals features of base triplet stepping that are conserved among these three phylogenet

276 Profiles were measured by stepping the confocal probe volume through the SLG/SiO(2

277 try (SCV), current transients acquired after stepping the potential along the redox wave of interest

278 Stepping threshold forces and in-place postural response

279 ysis finds that a number of proteins fold by stepping through a reproducible sequence of native-like

280 l triggering and other changes in protective stepping to impact fall risk.

281 Stepping towards this goal of incorporating biochemical

282 Non-stepping training also exacerbated some SCI-driven plast

283 and [T] can be quantitatively measured from stepping trajectories with very little prejudice in data

284 eck-linker element that is crucial for motor stepping undergoes discrete, ordered displacements.

285 Scientists are stepping up like never before to support science in the

286 rovide evidence for the clinical benefits of stepping up patients with COPD from an inhaled corticost

287 Many countries in Africa are stepping up their preparedness to detect and cope with C

288 models demonstrated clear redundancy between stepping variables, but could not replicate human steppi

289 tion of molecular motor properties including stepping velocity and characteristic run length.

290 y and membrane viscosity, reaching up to the stepping velocity of single motors.

291 ks that were specifically trained (standing, stepping, volitional leg movement).

292 plete section (T13) in these cats, bilateral stepping was seen on the next day, a skill usually acqui

293 To examine the role of the NL in regulating stepping, we investigated NL mutants of various lengths

294 To resolve rotations during stepping, we used optical tweezers combined with an opti

295 Instead, we find that hTpx2 inhibits hKif15 stepping when microtubule-bound.

296 een well described that prolonged rotational stepping will adapt the podokinetic sense of rotation, t

297 minimal ATPase cycle), the model reproduces stepping with 8-nm steps (even though the motor itself i

298 teral left hemisection (T10), cats recovered stepping with both hindlimbs within 3 weeks.

299 patterns without bilateral gait training, as stepping with one leg can facilitate adaptive learning t

300 ugh a series of experiments, we observe that stepping with only one leg can facilitate learning of an