ライフサイエンスコーパス: streptavidin

1 th 70-fold faster dissociation kinetics than streptavidin.

2 des that mimic key interactions of biotin to streptavidin.

3 using gold nanoparticles (AuNPs), biotin and streptavidin.

4 rties, and strong interaction of biotin with streptavidin.

5 s M13 bacteriophage high selectivity for the streptavidin.

6 covalently attached to lysines of wild-type streptavidin.

7 rmation, and panned against the model target streptavidin.

8 ded with CD200 extracellular domain and core streptavidin.

9 re force stable than commercial, multivalent streptavidin.

10 inity purification of the final adduct using streptavidin.

11 ected or minus-end-directed kinesin fused to streptavidin.

12 tion, decreasing k(off) 260-fold relative to streptavidin.

13 trated in reductive amination reactions with Streptavidin.

14 h low and high dissociation constant: biotin/streptavidin (10 fM) and HER2/HER2 antibody (0.44 +/- 0.

15 otin and staining with fluorescently labeled streptavidin, a large range of biomolecules can be image

16 method to stepwise evolve peptide binders to streptavidin, a protein studied for over two decades and

17 incubated with increasing concentrations of streptavidin, achieving a limit of detection (LOD) of 5n

18 A standard biotin-streptavidin affinity model was tested using the MGNT ar

19 specific binding affinity of the NCs toward streptavidin after incorporation.

20 Streptavidin-alkaline phosphatase was then conjugated to

21 These forms of streptavidin allow this key hub to be used with a new le

22 mo-genetic optimization of both cofactor and streptavidin allowed optimizing the performance of the h

23 Results show that filling the MGNT with streptavidin altered the effective refractive index of t

24 oxyphenyl-functionalized SPCEs modified with streptavidin and a sandwich type immunoassay was impleme

25 Although high affinity binding between streptavidin and biotin is widely exploited, the accompa

26 es to pretargeting, strategies predicated on streptavidin and biotin, bispecific antibodies, compleme

27 eter of 20 mum are first functionalized with streptavidin and biotinylated antibodies and then used t

28 a 2 x 2 junction array, functionalized with streptavidin and biotinylated antibodies specific for Es

29 Specifically, the competitive binding of streptavidin and goat anti-biotin for biotin-conjugated

30 ding silica nanoparticles (SNPs) coated with streptavidin and gold nanoparticles (AuNPs).

31 irect in situ breaks labeling, enrichment on streptavidin and next-generation sequencing (i-BLESS), w

32 oach based on the controlled binding between streptavidin and target activated affinity-switchable bi

33 age libraries to the N- or C-termini of core streptavidin and used them to setup phage-free noncompet

34 DNA anchors we attached a spherical protein (streptavidin) and a rod-shaped DNA (47bp) to a quartz cr

35 ntional fluorescently labeled antibodies and streptavidin, and fluorescent proteins.

36 ed by the covalent attachment of antibodies, streptavidin, and oligonucleotides and the binding and b

37 followed by the layer-by-layer deposition of streptavidin, and then biotynilated capture probes.

38 of labeled biomolecules such as antibodies, streptavidin, and tubulin proteins and showed that stabl

39 effectiveness by quantification of proteins (streptavidin, anti-digoxigenin, anti-tacrolimus) and sma

40 HA) amplification coupling with programmable streptavidin aptamer (SA-aptamer).

41 e oxidase, DNA/hydrogen peroxide, and biotin/streptavidin, are measured by the paper-based micro-calo

42 and analogues, with their strong binding to streptavidin, are used in many clinical laboratory tests

43 However, the introduction of streptavidin as a linker to the capture nanobody at the

44 a new desthiobiotin surface, using wild-type streptavidin as a robust bridge between the chip and the

45 By using streptavidin as a template and acylboronates and O-acylh

46 The detection of streptavidin at a low concentration of 0.1 ng/mL (~1.67

47 A towards a small peptide substrate carrying streptavidin at its distal end was also investigated to

48 The limiting step at present is binding of streptavidin at the end of DNA to biotin on capture bead

49 res of native-like cations of serum albumin, streptavidin, avidin, and alcohol dehydrogenase were pro

50 in-protein systems: biotin-avidin and biotin-streptavidin, barstar-dibarnase and Z domain-immunoglobu

51 f biotinylation sites identified compared to streptavidin-based enrichment of proteins.

52 neighborhood could then be characterized by streptavidin-based purification and mass spectrometry.

53 re optionally sheared, affinity-purified via streptavidin bead immobilization, and subjected to tradi

54 and biotinylated proteins are enriched with streptavidin beads and identified by mass spectrometry.

55 boID or split-TurboID are then enriched with streptavidin beads and identified by mass spectrometry.

56 and tagged RNA is biotinylated, captured on streptavidin beads and sequenced.

57 magnitude higher than previous results using streptavidin beads and the limit of detection (LOD) impr

58 tagged by biotin linkers and captured using streptavidin beads before library production and high-th

59 NA cross-linked to proteins were captured on streptavidin beads.

60 and releasing of biotinylated peptides from streptavidin beads.

61 labeled streptavidin is used to quantify the streptavidin binding capacity of each mesh type through

62 ptide Strep-tag II in a manner comparable to streptavidin binding to biotin.

63 body-epithelial cell surface-binding, biotin-streptavidin binding, and the topologically enhanced cel

64 plemental biotin ingestion may affect biotin-streptavidin binding, leading to potential clinical misi

65 horseradish peroxidase (HRP) through biotin-streptavidin binding.

66 HIV reporter virus (HIV-AFMACS) displaying a streptavidin-binding affinity tag at the surface of infe

67 cells (i) an organellar protein fused to the streptavidin-binding peptide (SBP) and (ii) motor, neck,

68 within or immediately after a 38-amino acid streptavidin-binding peptide (SBP) that is appended to t

69 ld-type AR tagged at its N terminus with the streptavidin-binding peptide epitope (streptavidin-bindi

70 says using truncated kinesin-1 motors with a streptavidin-binding peptide tag that can attach to stre

71 th the streptavidin-binding peptide epitope (streptavidin-binding peptide-tagged wild-type androgen r

72 hNAs, and the directed evolution of specific streptavidin-binding phNA aptamer ligands directly from

73 Strep-Tactin, an engineered form of streptavidin, binds avidly to the genetically encoded pe

74 g technology (SMT)-produced electrodes and a streptavidin biomediator currently display the highest r

75 ared trastuzumab conjugated to emtansine via streptavidin-biotin (Trastuzumab-SB-DM1) to the clinical

76 material arrays to biosensing using standard streptavidin-biotin affinity model.

77 conjugated via biotinylated glycan (through streptavidin-biotin affinity) followed by linkage of hem

78 Both streptavidin-biotin and SATA/SMCC conjugation chemistrie

79 neral tool which enables unrestricted use of streptavidin-biotin biotechnology in cellular uptake.

80 ) is introduced to exploit the full power of streptavidin-biotin biotechnology in cellular uptake.

81 c beads (MB) and subsequently, connected via streptavidin-biotin bonds to GOx.

82 ics and high-speed force spectroscopy on the streptavidin-biotin complex to determine the binding str

83 path caused by ligand binding, as shown with streptavidin-biotin complexes.

84 mbinations for activity and safety, based on streptavidin-biotin conjugation.

85 ike receptor-9 ligand-loaded particles using streptavidin-biotin cross-linking.

86 e complex immunoassays, including the use of streptavidin-biotin detection strategies.

87 bioreceptor layer was formed using a common streptavidin-biotin immobilization strategy and employed

88 and QD, and the high binding affinity of the streptavidin-biotin interaction, we achieved multiplexed

89 ce of MT minus ends labeled with dsDNA via a streptavidin-biotin interaction.

90 oated substrates and a strong but reversible streptavidin-biotin linkage to PEG-coated AFM tips enhan

91 in the limit-of-detection, the inclusion of streptavidin-biotin simplifies the development of simila

92 rter to its target organelle, relying on the streptavidin-biotin system.

93 we found that integrin receptors dissociate streptavidin-biotin tethered ligands in focal adhesions

94 Based on the observation of streptavidin-biotin unbinding, we also conclude that the

95 Streptavidin-biotin was chosen as a model system for eva

96 nition (e.g., antigen-antibody, DNA-DNA, and streptavidin-biotin) is a generic, yet highly versatile

97 ncentrations using a standard affinity model streptavidin-biotin.

98 advantages over traditional systems such as streptavidin-biotin.

99 rival widely used conjugation pairs, such as streptavidin-biotin.

100 The association constant (KA) for streptavidin/biotin and STV-NPs/biotin interactions obse

101 article enhancement, supersandwich assembly, streptavidin/biotin complex, antibody amplification, enz

102 promising advance toward synthetic mimics of streptavidin/biotin.

103 representative protein-ligand binding pairs (streptavidin/biotin; IgG/anti-IgG) were quantified.

104 ayer surface (SAMs) was used for immobilized streptavidin-biotinylated probes on the sensor surface f

105 The biotin-streptavidin bond is the strongest noncovalent bond in n

106 obing the force-dependent lifetime of biotin-streptavidin bonds, we find that monovalent streptavidin

107 The model protein streptavidin bound to horseradish peroxidase (HRP) was s

108 mate focusing position and separation of the streptavidin-bound biotin, anti-biotin-bound biotin, and

109 of biotinylated capture antibodies through a streptavidin bridge.

110 The streptavidin-bridged double-nanobody ELISA was then succ

111 MNPs coated with streptavidin can combine with biotin labeled thrombin ap

112 Finally, lateral flow strips with a streptavidin capture test line and an anti-antibody cont

113 thus avoiding the general paradigm of biotin-streptavidin chemistry and iii) a microfluidic platform

114 ) the classical assay that works with biotin-streptavidin chemistry, (II) the rapid assay that is per

115 s were immobilized on the surface via biotin/streptavidin chemistry.

116 A bioanalytical workflow involving streptavidin chromatography and label-free quantitative

117 ctionalized (4FB) magnetic beads rather than streptavidin coated beads with a high density of capture

118 ement of optical read-out was obtained using streptavidin coated gold-nanoparticles interacting with

119 ary anti-S.aureus aptamer was immobilized on streptavidin coated magnetic beads (MB), which serves as

120 rotocol based on biotinylated DNA probes and streptavidin coated magnetic beads we were able to selec

121 man capture mAb fragment in combination with streptavidin coated magnetic beads, providing capture ef

122 nt of a 384-well immuno-PCR method that uses streptavidin coated on a PCR plate to capture complexes

123 ich assay, which employed nanoEnhancers (NIR-streptavidin coated quantum dots) for ultrasensitive det

124 The biotinylated mAb, streptavidin coated silver nanoparticles (AgNPs) and hor

125 rum with a fully automated platform based on streptavidin coated tips and a biotinylated mouse anti-h

126 duct can be trapped and purified directly in streptavidin coated wells.

127 By measuring the diffusion of 400 nm streptavidin-coated fluorescent nanoparticles imaged at

128 The streptavidin-coated gold nanoparticles (AuNPs) were used

129 usly captured bacteria, and (III) binding of streptavidin-coated gold nanoparticles to the biotinylat

130 lated anti-human EGFR Apt was immobilized on streptavidin-coated magnetic beads (MB) and served as a

131 apture of biotinylated ligation junctions on streptavidin-coated magnetic beads and PCR amplification

132 lution and thus were further integrated into streptavidin-coated magnetic beads for purification of V

133 mits labeling cells of interest by attaching streptavidin-coated magnetic beads to their membranes us

134 drug and catabolites were released from the streptavidin-coated magnetic beads, separated by monolit

135 cted by scanning the microarray surface with streptavidin-coated magnetic beads.

136 rase reaction products, which immobilized on streptavidin-coated microplate, hybridized with biotinyl

137 rinted in an array format onto the bottom of streptavidin-coated microtiter wells.

138 on binding, the complex was harvested on the streptavidin-coated microwell, and subsequently the form

139 synthesized glycoconjugates were arrayed on streptavidin-coated plates and used to investigate the G

140 ling nanofiber meshes outperform traditional streptavidin-coated polystyrene plates under flow, valid

141 rategy that is enabled by the combination of streptavidin-coated quantum dot (QD) acceptors and bioti

142 d with the human adenosine deaminase (hADA1)-streptavidin complex and adenosine as a detection system

143 Experiments with the biotin-streptavidin complex show that the predicted three-dimen

144 uding 23 assays that incorporated biotin and streptavidin components and 14 assays that did not inclu

145 nd 14 assays that did not include biotin and streptavidin components and served as negative controls.

146 against matched targets (carbonic anhydrase, streptavidin, concanavalin A) to identify desired ligand

147 e native structures of the protein complexes streptavidin, concanavalin A, and C-reactive protein und

148 beta-lactoglobulin and enolase), tetrameric (streptavidin, concanavalin A, and pyruvate kinase), and

149 tection from 10 nmol L(-1) to 320 pmol L(-1) streptavidin concentration with a much higher sensitivit

150 final incubation of an Alkaline Phosphatase-streptavidin conjugate (ALP-Strp).

151 otinylated detection antibodies, fluorescent streptavidin conjugate, and wash buffer for a total volu

152 Furthermore, optical signal enhancement with streptavidin conjugated quantum dots was shown to yield

153 ng the plasma membrane of living cells using streptavidin conjugated with multiple fluorescent dye re

154 using secondary biotinylated antibodies and streptavidin-conjugated Au nanoparticles.

155 ction, which allows further elaboration with streptavidin-conjugated dyes or other molecules.

156 arbon electrode while is further linked to a streptavidin-conjugated HRP reporter.

157 lls, allowing facile one-step selection with streptavidin-conjugated magnetic beads.

158 s using biotinylated antibodies labeled with streptavidin-conjugated Pb- and Cd-based quantum dots (Q

159 et oligonucleotide followed by reaction with streptavidin-conjugated PbS QDs.

160 ion probe, hybridization was evidenced using streptavidin-conjugated to horseradish peroxidase (HRP)

161 Streptavidin-conjugated UCNPs were prepared by click rea

162 tramer technology and commercially available streptavidin conjugates.

163 -streptavidin bonds, we find that monovalent streptavidin constructs with specific attachment geometr

164 It was found that streptavidin could inhibit the binding of goat anti-biot

165 by varying the gap size, AuNP diameter, and streptavidin coverage on AuNPs.

166 In this report, cell-penetrating streptavidin (CPS) is introduced to exploit the full pow

167 geometry of the lipid layers, nucleation of streptavidin crystals occurred specifically on the DPN-p

168 alyzer based on the real-time measurement of streptavidin detection results from two-site binding mod

169 sensitivity and limit of detection (LOD) of streptavidin detection with the optimized parameters wer

170 nal between the biotin-bound Eu(III)-labeled streptavidin donor and the cysteine-coupled AlexaFluor 6

171 By labelling a DNA replication fork with streptavidin (dsDNA end) and Fab (5' ssDNA) we located t

172 The model was tested with a streptavidin-dsDNA complex linked through biotin (small

173 while the ASB probe would not bind with the streptavidin-enzyme conjugate due to its low binding aff

174 ts, the biotinylated protein and HRP-labeled streptavidin for its detection.

175 h biotin to form a noncovalent link with the streptavidin functionalized AFM tip during the approach

176 meat marker using biotinylated reporter and streptavidin functionalized gold nanostars (Stre@GNSs).

177 linkers (BLs) to mediate the aggregation of streptavidin-functionalized gold nanoparticles (st-AuNPs

178 ffinity and the chemical characterization of streptavidin-functionalized gold nanoparticles (STV-NPs)

179 ed oligonucleotides) and (ii) the capture of streptavidin-functionalized gold nanoparticles to the af

180 rty of CD200 for surface modification, CD200-streptavidin fusion protein was expressed from bacteria

181 d lateral flow assay was based on the use of streptavidin gold nanoparticles for the labelling of the

182 High-affinity interactions of streptavidin-gold nanoparticles (strep-AuNPs) to the bio

183 Streptavidin has the advantage of rapid ultra-stable bin

184 ) the biotinylated detection antibody, (iii) streptavidin horseradish peroxidase, (iv) a wash buffer,

185 was then revealed by the introduction of the streptavidin-horseradish peroxidase conjugate that catal

186 er labeling the hybridized biotin-miRNA with streptavidin-HRP conjugates, amperometric detection at -

187 ddition, more detailed results presented for streptavidin illustrate the ability of the method to exh

188 lting from successful binding of d-biotin to streptavidin immobilized on functionalized acrylate hydr

189 n-labeled terminal of the probe bound to the streptavidin immobilized on the lateral flow test strip,

190 en-specific antibody levels were measured in streptavidin ImmunoCAPs loaded with each of the recombin

191 and 100 mM triethylammonium acetate and for streptavidin in 200 mM ammonium acetate with no charge r

192 The focusing position of streptavidin in electrophoretic-electroosmotic focusing

193 odel multivalent molecule, biotinylated anti-streptavidin, in PBS.

194 njugate due to its low binding affinity with streptavidin, in the presence of the target analyte, the

195 ti-IL-17A catch antibodies, which via biotin-streptavidin interaction are bound to the biotinylated s

196 The SBP-streptavidin interaction drives accumulation of organell

197 argeting of tumors through the strong biotin-streptavidin interaction.

198 alization strategy was connected with biotin-streptavidin interactions to demonstrate the capabilitie

199 conjugated to the MMC surface through biotin-streptavidin interactions.

200 Fluorescently labeled streptavidin is used to quantify the streptavidin bindin

201 e functional groups-achieved upon binding to streptavidin-is critical for the observed enhancement in

202 t of the biotin-tagged tryptic peptides with streptavidin; (iv) liquid chromatography-tandem MS (LC-M

203 We have engineered streptavidin labelled Europium doped fluorescent silica

204 A ~400 fM streptavidin limit of detection is expected with a 0.01n

205 roach stage before the rupture of the biotin-streptavidin link.

206 luated internalization by target cells using streptavidin-linked antibodies conjugated to biotinylate

207 vidin-binding peptide tag that can attach to streptavidin-loaded, supported lipid bilayers.

208 products by lambda-exonuclease digestion and streptavidin magnetic bead isolation.

209 e" or "dual capture" of ADCs from serum with streptavidin magnetic beads coated with a generic biotin

210 ted sense-strand oligonucleotides coupled to streptavidin magnetic beads.

211 ed and detected by the GMR sensor by linking streptavidin magnetic nanoparticles (MNPs) to the sensor

212 nvolving a sandwich hybridization assay onto streptavidin-magnetic beads (Strep-MBs), hybridization c

213 get DNA, captured through hybridization onto streptavidin-magnetic microbeads (Strep-MBs) modified wi

214 First, the streptavidin-magnetic nanobeads (MBs) were functionalize

215 Streptavidin-magnetic nanoparticles (streptavidin-MNPs)

216 ated cells, DNA end-labeling with biotin and streptavidin-mediated chemiluminescent detection of the

217 wide variety of molecules including dextran, streptavidin, microspheres, and lentivirus particles.

218 Streptavidin-magnetic nanoparticles (streptavidin-MNPs) are premixed with LAMP reagents inclu

219 d on the glassy carbon electrode (GCE) using streptavidin modified-gold nanoparticles/thiolated graph

220 enhanced Raman spectroscopy (MA-SERS) using streptavidin-modified magnetic nanoparticles (MNP@Strep)

221 As a platform for isolation process, streptavidin-modified MPs, which were conjugated via bio

222 , and finally allows the immobilization of a streptavidin-modified ruthenium-based ECL label via reac

223 oups, enabling the molecule to be labeled by streptavidin molecules at these sites.

224 th a central scaffold protein linked to four streptavidin molecules, each having three pMHC ligands o

225 zol-4-yl)(biotin-cap-NBD-PE) lipids and that streptavidin more effectively outcompeted the anti-bioti

226 relying on fluorophore-conjugated monomeric streptavidin (mSA) to label membrane proteins carrying a

227 We report that a monomeric streptavidin (mSav) Rh(III) ArM permits asymmetric synth

228 how that cross-linking of biotinylated HA in streptavidin multimers or supramolecular complexes with

229 e loop critical for biotin binding, creating streptavidin muteins (M88 and M112) with novel disulfide

230 e first validate the method using artificial streptavidin-oligonucleotide complexes, followed by anal

231 held even when the DNA duplex is tethered to streptavidin or the mobility of the nitroxides is altere

232 oteins cholera toxin B subunit-Alexa 647 and streptavidin-PE/Cy5, to membranes containing different a

233 have introduced a UCNP label design based on streptavidin-PEG-neridronate and a two-step detection sc

234 ally labelled with a commercial conjugate of streptavidin-peroxidase (Strep-HRP) as tracer.

235 Upon hybridization, a streptavidin-peroxidase (Strep-HRP) conjugate was employ

236 e reaction with the electrochemical reporter streptavidin-peroxidase conjugate.

237 s the binding of gliadin after incubation in streptavidin-peroxidase.

238 When desired, however, streptavidin plus the biotinylated bait can be completel

239 -TGF and conjugation with peroxidase-labeled streptavidin (poly-HRP-Strept) polymer.

240 ted secondary antibodies, which then capture streptavidin-poly [horse radish peroxidase] (Poly-HRP).

241 alized plasmonic AuNSs substrate but against streptavidin previously conjugated to gold nanorods, the

242 strains engineered for enhanced tyrosine or streptavidin production.

243 ollowing PVP removal, biotinylated thiol and streptavidin protein were added to the nanostars, which

244 A biotin-streptavidin pull-down assay showed that 4,11-bis(2-amin

245 by confocal microcopy after incubation with streptavidin quantum dots.

246 to release biotin-tagged DNA constructs from streptavidin rapidly and efficiently.

247 on of S112 reveals a marked effect of the Ir/streptavidin ratio on both the saturation kinetics as we

248 e (LSPR) shift-based biosensors using biotin-streptavidin recognition interaction as a proof-of-conce

249 DeNAno were selected against streptavidin-, rituximab- and bevacizumab-coated beads.

250 The strong interaction between streptavidin (SA) and biotin is widely utilized in biote

251 advance is made possible by incorporating a streptavidin (SA) hydrogel capture/purification element

252 RP) for catalytic colorimetric assay and for streptavidin (SA)-biotin immunoassays.

253 Streptavidin (SA)-biotin pretargeted radioimmunotherapy

254 CD38 bispecific proved equal or superior to streptavidin (SA)-biotin-based CD38-SA PRIT.

255 ular domain of CD47 modified to include core streptavidin (SA).

256 amples of blank ImmunoCAPs coupled to either streptavidin (SA-CAP-1 or 2) or nonallergenic maltose-bi

257 ammed cell death 1 ligand chimeric with core streptavidin (SA-PDL1) that inhibited the T effector cel

258 ts versatility, the homotetrameric nature of streptavidin (Sav) and the noncooperative binding of bio

259 To address these questions, we study streptavidin (SAv) binding to commonly used biotinylated

260 strate that a cysteine-containing variant of streptavidin (Sav) can serve as a protein host to model

261 by developing a sequence-independent biotin-streptavidin (SAv) roadblocking strategy that simplifies

262 Using biotin-streptavidin (Sav) technology, artificial copper protein

263 Importantly, competition of the streptavidin-SBP interaction by the addition of biotin t

264 engineer a (monovalent) single-chain dimeric streptavidin (scdSav) as scaffold for Sav-based ArMs.

265 ntigens pretreated with different bispecific streptavidin-scFv fusion proteins.

266 Similarly, streptavidin sensing shows the lowest detection of 1ngml

267 iotinylated liposomes to surface-immobilized streptavidin show that the refractive index of the encap

268 by several experiments including fluorescent streptavidin staining, delayed competition in affinity p

269 We developed a modular RNA aptamer-streptavidin strategy, termed S1mplex, to complex CRISPR

270 e we report the solution of a selenobiotinyl-streptavidin structure using phases obtained by the anom

271 amers for malachite green (MG), spinach, and streptavidin (STV).

272 with biotin, which was used as a ligand for streptavidin (SV) detection.

273 The biotin-streptavidin technology has been extensively exploited t

274 eterologous cargos along DNA, for example, a streptavidin tetramer.

275 nd 14x increase of CID sensitivity of 53 kDa streptavidin tetramer.

276 When bound to streptavidin, this compound cannot cross the plasma memb

277 sive activated SA-aptamers could capture the streptavidin to achieve enhancement and output of the de

278 be used as cofactors for incorporation into streptavidin to assemble artificial hydroxylases.

279 biotinylated liposomes are attached through streptavidin to biotinylated capture probes.

280 Here, we leveraged the unique affinity of streptavidin to develop a mouse infection model with hum

281 and conjugated them to antibodies linked to streptavidin to evaluate antitumour activity and pre-cli

282 ferent vertexes and is further assembled via streptavidin to form the multivalent DTP (SA-DTP).

283 , which can then bind with the enzyme-tagged streptavidin to initiate signal amplification process.

284 The binding of coexpressed streptavidin to SBP causes signal masking, whereas addit

285 )-biotin/horseradish peroxidase (HRP)-biotin/streptavidin to the poly(A) tails, and the oxidation rea

286 ation of three tetrameric protein complexes (streptavidin, transthyretin, and hemoglobin) in the gas

287 phycoerythrin-biotin (PhycoE-Biotin) and Cy5-streptavidin trapped in the two proteoliposome populatio

288 Rupture forces comparable to biotin:streptavidin unbinding were observed.

289 te states far from the binding pocket, while streptavidin undergoes transient induced fits, all varyi

290 n detecting a high molecular weight analyte (streptavidin) versus in the flow-over PSi approach.

291 on (LOD) of the MGNT arrays for detection of streptavidin was estimated as 25nM, with a detection tim

292 inylated reporter antibody and reaction with streptavidin was selected.

293 atch crystallization screening setups, where streptavidin was used as a model protein for crystalliza

294 As a result, the H1-H2 complexes and streptavidin were attached to the sensor surface, leadin

295 rations (4.17pM to 41.7nM) of dye-conjugated streptavidin were simultaneously infused through the sec

296 lymers with the ability to specifically bind streptavidin while minimizing the nonspecific binding of

297 100% from both solution-phase and bead-bound streptavidin with as little as 12% (v/v) phenol, leaving

298 covered with carboxylated silica, biotin, or streptavidin with recovery rates of 30 to 50%.

299 e have achieved 2.6- angstrom resolution for streptavidin, with a molecular weight of 52 kDa, from 11

300 y and 4,7-dihydroxy-1,10-phenanthroline into streptavidin yields an NAD(P)H-dependent artificial tran