ライフサイエンスコーパス: streptolysin

1 -dependent cytolysins (CDC), pneumolysin and streptolysin.

2 o Schwann-like STS26T cells permeabilized by streptolysin 0.

3 ts of PEG on zymosan, lipopolysaccharide, or streptolysin-induced inflammatory and bioenergetic respo

4 Streptolysin-induced necrosis of human neutrophils was r

5 d these processes required SpeB protease and streptolysin O (SLO) activities, respectively; and (c) G

6 ith CLI reduced extracellular DNase Sda1 and streptolysin O (SLO) activity in vivo, whereas subinhibi

7 articipate in CMT, the pore-forming molecule streptolysin O (SLO) and an effector protein with the ch

8 Two such proteins, streptolysin O (SLO) and NAD(+)-glycohydrolase (NADase),

9 Two such products, streptolysin O (SLO) and NAD+-glycohydrolase, appear to

10 Although the toxicity of streptolysin O (SLO) and streptolysin S (SLS) in purifie

11 NADase (SPN) and streptolysin O (SLO) are two toxins that play important

12 tudies identified the pore-forming cytolysin streptolysin O (SLO) as necessary and sufficient for the

13 including the SpeB cysteine protease and the streptolysin O (SLO) cytolysin, but not SIC, a protein t

14 ective epitopes using the cytolytic exotoxin Streptolysin O (SLO) from Streptococcus pyogenes as a sh

15 rearrangement in the upstream region of the streptolysin O (slo) gene of Streptococcus pyogenes whic

16 ssumes that the NAD-glycohydrolase (nga) and streptolysin O (slo) genes that code for these products

17 Streptolysin O (SLO) is a cholesterol-dependent cytolysi

18 cell lines using cationic lipid systems and streptolysin O (SLO) is used to effect their delivery.

19 duction of the cholesterol-binding cytotoxin streptolysin O (SLO) prevented internalization of GAS in

20 ed the abundance of streptolysin S (SLS) and streptolysin O (SLO) production between clinically domin

21 er permeabilization with the bacterial toxin streptolysin O (SLO) requires endocytosis via a novel pa

22 utilizes the cholesterol-dependent cytolysin Streptolysin O (SLO) to translocate the NAD(+) -glycohyd

23 utilizes the cholesterol-dependent cytolysin Streptolysin O (SLO) to translocate the NAD(+) glycohydr

24 athogen Streptococcus pyogenes that utilizes streptolysin O (SLO), a cholesterol-dependent cytolysin.

25 rulence factor that is present among most is streptolysin O (Slo), a protein with well-characterized

26 f the archetypical member of the CDC family, streptolysin O (SLO), a virulence factor from Streptococ

27 The GAS cholesterol-dependent cytolysin, Streptolysin O (SLO), has well established cell and tiss

28 bronectin-binding proteins (sfbI and fbp54), streptolysin O (slo), hyaluronic acid capsule (hasA), st

29 rol-binding CDCs, perfringolysin O (PFO) and streptolysin O (SLO), were found to exhibit strikingly d

30 We demonstrate that streptolysin O (SLO)-induced glutathione efflux from hos

31 easuring the properties of Ca2+ signaling in streptolysin O (SLO)-permeabilized cells were used to st

32 eted cytotoxins S. pyogenes NADase (SPN) and streptolysin O (SLO).

33 ion of the secreted pore-forming haemolysin, streptolysin O (SLO).

34 Ca2+ signalling after permeabilization with streptolysin O (SLO).

35 haride and/or large amounts of the cytotoxin streptolysin O (SLO).

36 ular toxins NAD+-glycohydrolase (NADase) and streptolysin O (SLO).

37 g the immunity factor IFS and the cytolysin (streptolysin O [SLO]), were more abundant in the mutant

38 ne response to SCPA correlated with the anti-streptolysin O and anti-DNase B responses.

39 ukin-6), and modest serological responses to streptolysin O and deoxyribonuclease B.

40 lored various methods including TAT peptide, Streptolysin O and microporation for delivering NeutrAvi

41 enting binding of the sterol-specific toxins streptolysin O and perfringolysin O and subsequent cytot

42 ll calcium ion-dependent repair responses to streptolysin O and perfringolysin O, but only 50% of rep

43 Streptolysin O damaged and killed the cells quickly, all

44 ion of toxic shock syndrome toxin-1, whereas streptolysin O directly damages the mucosa to allow for

45 e pores are produced by perfringolysin O and streptolysin O during insertion (and not small pores tha

46 alpha-toxin and the streptococcal cytolysin streptolysin O enhanced penetration of toxic shock syndr

47 While reports have linked sloR function to streptolysin O expression, transport experiments with ra

48 Permeabilization with streptolysin O for 10 minutes permitted the loss of free

49 A similar analysis conducted on streptolysin O from Streptococcus pyogenes revealed that

50 alpha-hemolysin from Staphylococcus aureus, streptolysin O from Streptococcus pyogenes, and anthroly

51 When delivered with streptolysin O into living human epithelial cancer cells

52 ected host cell via the pore-forming protein streptolysin O is unknown.

53 ed by bacterial pore-forming toxins, such as streptolysin O or perfringolysin O, during septic cardio

54 were introduced to HL-60 cells by use of the streptolysin O pore-forming peptide.

55 S. pyogenes-derived c-di-AMP diffuses via streptolysin O pores into macrophages where it activates

56 by TLR2 and TLR4 ligands in the presence of streptolysin O required Myd88/Trif, whereas that induced

57 Streptolysin O treatment of infected human macrophages i

58 was similar to mga, i.e., slo (which encodes streptolysin O) and plr (encoding the plasmin receptor/g

59 or) but did not affect transcription of slo (streptolysin O), mga (multiple gene regulator of GAS), e

60 between patient groups with nonfilarial Ag (streptolysin O)-stimulated supernatant (LP = 0.160 relat

61 mmalian cells has been achieved by employing Streptolysin O, a bacterial enzyme which forms temporary

62 nst another cholesterol-dependent cytolysin, streptolysin O, and protected lung epithelial cells and

63 hils, that this activity was attributable to streptolysin O, and that platelet/neutrophil complex for

64 by collagenase digestion, permeablized with streptolysin O, and the release of Ca2+ from internal st

65 requires NF-kappaB and the virulence factor streptolysin O, but proceeds independently of P2X7R and

66 tion of antibodies to HlpA and antibodies to streptolysin O, indicating that the histone-like protein

67 In MIN6 beta-cells permeabilized by streptolysin O, insulin release was stimulated by elevat

68 omosomal region encoding secreted NADase and streptolysin O, is the key driver of increased toxin pro

69 e genes encoding streptokinase, CAMP factor, streptolysin O, M protein (more abundant in the CvfA(-)

70 n of other extracellular products, including streptolysin O, streptokinase, and DNase, was not affect

71 A when delivered into P815 target cells with streptolysin O, whereas transfection of target cells wit

72 Examples include the pore-forming cytotoxin streptolysin O, which oligomerises to form large pores i

73 t NAD-glycohydrolase is translocated through streptolysin O-generated pores into a host cell.

74 t ceramide reduced perfringolysin O- but not streptolysin O-mediated cytotoxicity in cells.

75 The Streptolysin O-Percoll (SLOPE) method is effective on pr

76 in or addition of Ca2+-containing buffers to streptolysin O-permeabilized cells induced exocytosis of

77 um) of isolated microsomes, Ca2+ uptake into streptolysin O-permeabilized cells, and analysis of SERC

78 In streptolysin O-permeabilized cells, guanosine 5'-O- (2-t

79 t vastly different transport capabilities in streptolysin O-permeabilized cells, in accordance with t

80 secretion when introduced after docking into streptolysin O-permeabilized cells; so does a Rab3A-22A

81 pendent manner, both in vitro and in vivo in streptolysin O-permeabilized chromaffin cells.

82 bility of inducers to elicit exocytosis when streptolysin O-permeabilized human sperm were loaded wit

83 y of SCAMP2, potently inhibits exocytosis in streptolysin O-permeabilized mast cells.

84 dent fashion, as shown using both intact and streptolysin O-permeabilized parasites.

85 tron microscopy and proteolytic digestion of streptolysin O-permeabilized parasitized erythrocytes.

86 Using a streptolysin O-permeabilized platelet exocytosis assay,

87 d in alpha-granule secretion, we developed a streptolysin O-permeabilized platelet model of alpha-gra

88 Streptolysin O-permeabilized platelets released alpha-gr

89 Streptolysin O-permeabilized platelets synthesized PtdIn

90 Exposure of streptolysin O-permeabilized platelets to antihuman cell

91 Incubation of streptolysin O-permeabilized platelets with an antibody

92 Incubation of streptolysin O-permeabilized platelets with phosphatidyl

93 -2, rVAMP-3, and rVAMP-8 were incubated with streptolysin O-permeabilized platelets.

94 ed Ca2+-induced alpha-granule secretion from streptolysin O-permeabilized platelets.

95 In streptolysin O-permeabilized PMNs, PLD was directly acti

96 Streptolysin O-permeable pancreatic acini were used to s

97 ed wild-type amounts of type 3 M protein and streptolysin O.

98 ly permeabilised with the pore-forming toxin streptolysin O.

99 od of plasma membrane permeabilization using streptolysin O.

100 e Dynasore also protected HeLa cells against streptolysin O.

101 d to a structurally similar bacterial toxin, streptolysin O.

102 ng secreted toxins NAD(+)-glycohydrolase and streptolysin O.

103 i) reversible membrane permeabilization with streptolysin O.

104 tory effect of an unrelated cytolytic toxin, streptolysin O.

105 equired expression of the pore-forming toxin streptolysin O.

106 ing enhanced by the effects of M protein and streptolysin O.

107 sed for the homologous member of the family, streptolysin O.

108 ludes listeriolysin O, perfringolysin O, and streptolysin O.

109 mparable and significant median responses to streptolysin-O (0.65 log10 AU/mL; interquartile range [I

110 ided that cells are first permeabilized with Streptolysin-O (SL-O), and (2) chimeric methylphosphonod

111 using either cells gently permeabilized with streptolysin-O (SLO) or microsomes from homogenized cell

112 her streptococcal cell wall antigen (SCW) or streptolysin-O (SLO) to augment lung injury in rats.

113 In streptolysin-O (SLO)-perforated rat brain cortical synap

114 dies recognizing specific domains of apoB in streptolysin-O (STP-O)- and saponin-permeabilized HepG2

115 kidney (PK-15) cells were permeabilized with streptolysin-O and incubated with a library of 125I-labe

116 and permeabilization of cultured acini with streptolysin-O and insertion of GDP beta S or antibodies

117 Permeabilization by streptolysin-O and introduction of guanosine thiodiphosp

118 ization of plasma membranes by detergents or streptolysin-O in hepatocytes, thymocytes, and P19, Jurk

119 Mast cells permeabilized with streptolysin-O leak soluble (cytosol) proteins over a pe

120 Streptolysin-O permeabilization was used to introduce an

121 phate via the recording patch pipette or via streptolysin-O permeabilization.

122 an antagonist of Gq/11, was introduced into streptolysin-O permeabilized acini to bypass the plasma

123 When streptolysin-O permeabilized CHO cells were incubated wi

124 concentration ([Ca2+]i) from both intact and streptolysin-O permeabilized isolated nerve endings of t

125 sopressin (AVP) secretion in both intact and streptolysin-O permeabilized nerve endings.

126 We have developed a novel assay system using Streptolysin-O permeabilized neutrophils that recapitula

127 ify this, we measured resting Ca2+ sparks in streptolysin-O permeabilized ventricular myocytes from w

128 Anti-streptolysin-O titres were elevated in 65% of patients.

129 were used in cultured acini permeabilized by streptolysin-O to determine the role of the G proteins i

130 ced into the cells via permeabilization with streptolysin-O, and cellular uptake was confirmed by flu

131 efect was preserved by permeabilization with streptolysin-O, it was determined that Lec35p is not dir

132 Streptolysin-O-mediated guanosine 5'-3-O-(thio)triphosph

133 ant CRHSP-28 (rCRHSP-28) was introduced into streptolysin-O-permeabilized acinar cells, and amylase s

134 late Ca(2+)-stimulated secretory activity in streptolysin-O-permeabilized acinar cells.

135 In streptolysin-O-permeabilized acini of transgenic mice, a

136 2+) uptake into the endoplasmic reticulum of streptolysin-O-permeabilized cells by sarco/endoplasmic

137 tion of function-blocking Sec8 antibodies to streptolysin-O-permeabilized cells revealed exocyst requ

138 tentiated InsP(3)-evoked Ca(2+) release from streptolysin-O-permeabilized cells.

139 with the slow leak rate of the protein from streptolysin-O-permeabilized cells.

140 ing in vitro also inhibits acid secretion in streptolysin-O-permeabilized gastric glands.

141 In streptolysin-O-permeabilized MDCK cells, Sec8 antibodies

142 the presence of the ionophore A23187 and in streptolysin-O-permeabilized semi-intact cells.

143 y permeabilized with the pore-forming toxin, streptolysin-O.

144 CvfA(-) mutant), SpeB, mitogenic factor, and streptolysin S (less abundant).

145 ated hasABC operon, streptokinase (ska), and streptolysin S (sagA) during growth in the presence of 1

146 Increased transcript levels of sagA and streptolysin S (SLS) activity during exponential-phase g

147 acid capsule synthesis and exotoxins, e.g., streptolysin S (SLS) and pyrogenic exotoxin B (SpeB).

148 ranscription of sagA, a gene associated with streptolysin S (SLS) and speB, the gene encoding pyrogen

149 We also compared the abundance of streptolysin S (SLS) and streptolysin O (SLO) production

150 et-activating factor acetylhydrolase Sse and streptolysin S (SLS) have synergistic contributions to i

151 ugh the toxicity of streptolysin O (SLO) and streptolysin S (SLS) in purified group A streptococci (G

152 Streptolysin S (SLS) is the cytolytic factor that create

153 The oxygen-stable hemolysin streptolysin S (SLS) of Streptococcus pyogenes is encode

154 Streptolysin S (SLS) produces the hallmark beta-haemolyt

155 Notably, the entire sag operon necessary for streptolysin S (SLS) production was under CcpA-mediated

156 ually all group A streptococci (GAS) produce streptolysin S (SLS), a cytolytic toxin that is responsi

157 nt phosphotransferase system (PTS) exhibited Streptolysin S (SLS)-mediated hemolysis during exponenti

158 elated genes that control biofilm formation, streptolysin S (SLS)-mediated hemolysis, and localized u

159 ccus sag operon encoding the beta-haemolysin streptolysin S (SLS).

160 factors produced by GAS is the peptide toxin streptolysin S (SLS).

161 es a powerful cytolytic bacteriocin known as streptolysin S (SLS).

162 s secretes a highly cytolytic toxin known as streptolysin S (SLS).

163 ficient in expression of the cytolytic toxin streptolysin S (SLS-) and evaluated events that occur du

164 e demonstrate the in vitro reconstitution of streptolysin S activity.

165 lting strain, JRS470, produced no detectable streptolysin S and showed a drastic reduction in cell su

166 resulted from enhanced transcription of the streptolysin S biogenesis operon.

167 a genetic locus immediately upstream of the streptolysin S biosynthetic operon in several GAS genome

168 Although it has been widely held that streptolysin S exerts its lytic activity through membran

169 For the streptolysin S hemolysin, a dramatic increase in hemolyt

170 sing high-resolution live cell imaging, that streptolysin S induces a dramatic osmotic change in red

171 ption of ska (encoding streptokinase), sagA (streptolysin S), and speMF (mitogenic factor) but did no

172 ag promoter, which directs the expression of streptolysin S, a hemolysin that can damage the membrane

173 hat resembled the biosynthetic machinery for streptolysin S, a key virulence factor from group A Stre

174 The amounts of streptokinase, streptolysin S, and capsule paralleled the levels of tra

175 ificantly reduced the haemolytic activity of streptolysin S, and dramatically reduced the pathology i

176 (capsule, cysteine protease, streptokinase, streptolysin S, and streptodornase).

177 s necessary and sufficient for production of streptolysin S, another GAS virulence factor, is also ne

178 n unrelated second streptococcal haemolysin, streptolysin S, during infection of keratinocytes.

179 d genes encode the key GAS virulence factors Streptolysin S, PrtS (IL-8 degrading proteinase), and Sp

180 otypes: decreased beta-hemolysis mediated by streptolysin S, reduction in the activity of a secreted

181 Skin infection sites of streptolysin S-deficient MGAS2221 DeltasagA were full of

182 ergrational mutagenesis demonstrate that the streptolysin S-like gene cluster from Clostridium sp. is

183 s provide key insights into the mechanism of streptolysin S-mediated haemolysis and have implications

184 ion of a small peptide toxin by GAS known as streptolysin S.

185 ence factors, including the potent cytolysin streptolysin S.

186 to the promoter region of the gene encoding streptolysin S.

187 cular insight into the chemical structure of streptolysin S.

188 The expression of the streptolysin toxins is strongly upregulated, whereas gen

189 during adherence to host cells, GAS delivers streptolysin toxins, creating endoplasmic reticulum stre