ライフサイエンスコーパス: subculture

1 smooth muscle cells in vivo and in vitro (in subculture).

2 ation assays were performed initially and at subculture.

3 cells placed in culture, and did not require subculture.

4 Many of the differences persist through subculture.

5 nonadherent, smooth phenotype upon in vitro subculture.

6 se obtained from panels inoculated following subculture.

7 ower, were transformed, and none reverted on subculture.

8 ansformed cells and a reduced growth rate on subculture.

9 0.5 McFarland suspension prepared from plate subculture.

10 res using MALDI-TOF and without the need for subculture.

11 ly from positive blood culture broth without subculture.

12 edium, growth temperature, and use of serial subculture.

13 m selective media and subsequently from pure subculture.

14 tly, typically at approximately 9 days after subculture.

15 (12.8%) were positive for S. agalactiae upon subculture.

16 The remaining stroma was digested and subcultured.

17 in G1 phase unless the irradiated cells are subcultured.

18 riod, and the resulting clones were serially subcultured.

19 resistant cells were selected, expanded, and subcultured.

20 ich arguably contributes to the formation of subcultures.

21 of pneumococcal bacteremia despite negative subcultures.

22 roducible between amplifications and between subcultures.

23 clone, but caught up to it after four weekly subcultures.

24 een religious and nonreligious societies and subcultures.

25 y shorter than those for identification from subcultures.

26 at found in wild type R. monacensis after 15 subcultures.

27 e two Ureaplasma species in culture-positive subcultures.

28 e adipogenesis and replication declined with subculturing.

29 at glucose broth or on sheep blood agar upon subculturing.

30 cells passed through stationary phase before subculturing.

31 side for 4 hours, and DNA was isolated after subculturing.

32 reus was drastically reduced with the fungal subculturing.

33 on to wild-type C. parasitica and successive subculturing.

34 the same cultures 1 day later and from fresh subcultures 2 months later.

35 he exon 2 + 3 deletion assessed at 6 days of subculture after 4 hours of 0, 0.25, 1, 2.5, 5, and 10 m

36 lture total mouse keratinocytes for up to 19 subcultures, allowing an increase in cell number of grea

37 isolates for 12 months without the need for subculture and confirmed the viability of all isolates b

38 of contamination due to the needs of routine subculture and dark field microscopy.

39 to be equally comfortable in their own peer subculture and not to be different in the proportion tha

40 wn about which integrins are involved during subculture and passage.

41 After treatment, the cells were subcultured and grown for 7 days in medium without [125I

42 sitive blot; four unidentified colonies were subcultured and serotyped by the Quellung reaction.

43 ation from blood and following 5, 10, and 20 subcultures and at 1, 3, 6, and 12 months of storage at

44 transiently express exogenous GFP up to six subcultures and for at least 2 mo after infection, witho

45 selection of mutants, reducing the number of subcultures and thereby minimising the likelihood of acq

46 sels to new axenic plants facilitates robust subculturing and produces hundreds of whitefly adults pe

47 Taxol yield of Aspergillus terreus with the subculturing and storage were the technical challenges t

48 ingle 0.1-ml sample from each clear tube for subculture, and adopting an alternate method for calcula

49 Bone tumors were subcultured, and chromosomal analysis demonstrated that

50 All fermenting specimens were subcultured, and isolates were tested for toxigenicity.

51 plated on CHROMagar Candida and RPMI medium, subcultured, and submitted for antifungal susceptibility

52 were recovered from Lowenstein-Jensen (BBL) subcultures, and 50 of the isolates were recovered from

53 n average of 5.99 population doublings after subculturing, and a lifespan from 21 to 35 days.

54 ere exponentially suppressed with A. terreus subculturing, and strongly restored upon addition of P.

55 hown that NO donors inhibit the migration of subcultured aortic smooth muscle cells.

56 Two of four isolates subcultured approximately 20 times ( approximately 500 c

57 At another 7 days, the growth in subculture at each time point was graded "1" for growth

58 ty of these age-related properties by serial subculture at low density of the two uncloned cultures a

59 d an acridine orange stain on day 8 and were subcultured at 2, 4, and 8 weeks.

60 Anaerobic BACTEC bottles were subcultured at 4 weeks.

61 ared as early as 4 weeks in culture and were subcultured at 8 weeks.

62 confluence, cells in KSFM were continuously subcultured at a 1-to-3 split.

63 pairment of proliferation when the cells are subcultured at low density and a greatly increased proba

64 made to results from prospective testing of subcultures at the Scottish MRSA Reference Laboratory, u

65 ase activity were generated by using freshly subcultured bacteria or by treating repeatedly subcultur

66 ification of OXA-48 family carbapenemases in subcultured bacterial isolates based on a genoproteomic

67 ee colonies) thereby preventing the need for subculturing bacterial isolates to reach a larger amount

68 Spirochetes could not be successfully subcultured by day 3 after exposure to ceftriaxone.

69 Cells were subcultured by transferring spheres to new culture dishe

70 nonliving macromolecules and transferred on "subculture" by self-propagating microcrystalline apatite

71 re, it is shown that memory effects of prior subculture can influence the variation of perturbation p

72 Subcultured cells could be cryopreserved and used to ino

73 Furthermore, phospho-ERK levels in subcultured cells from ARF6(GTP) and ARF6(GDP) tumor exp

74 d not alter basal or stimulated migration of subcultured cells, except at a relatively high concentra

75 ects on cell proliferation in primary versus subcultured cells, indicating fundamental differences am

76 re and contrast their response with those in subcultured cells.

77 s telomerase-deficient mutants were serially subcultured, cells exhibited a progressive decline in av

78 Subcultured colonies were identified by 16S rRNA gene se

79 on-related genes were upregulated in stunted subcultures compared with the Fgmcm1 mutant, which was d

80 ng spectral profiles generated under various subculture conditions, as well as with and without hVISA

81 Stimulation of subcultured CSMC with UTP, ITP, or ATP induced a concent

82 Repeated subculture demonstrated that 2 of 9 isolates (22.2%) exh

83 emperatures, growth medium types, and repeat subcultures did not result in misidentification.

84 This assay does not require subculturing, DNA purification, restriction digestion, S

85 The ability of the WASP to subculture enrichment broths was evaluated with 106 Lim

86 enotype was stably maintained after multiple subcultures even in the absence of antibiotic selection.

87 Importantly, the cell line is continuously subcultured every 10-14 days when split at a 1:3 ratio a

88 Serial subcultures every 4 to 5 days were required to maintain

89 Initial subculture failed, but the organism was identified as He

90 1 mutants were unstable and produced stunted subcultures, Fgmcm1 mat1-1-1 but not Fgmcm1 fst12 double

91 ong stimulators of collagenase expression in subcultured fibroblasts of all types, including those fr

92 eal myofibroblasts (PCMs) were obtained from subcultured fibroblasts plated at a low density (5 cells

93 hed MS culture, incubated overnight and then subcultured for an additional 24 h, the agreement with X

94 Unidentified isolates were subcultured for repeat testing; 71/319 (22.3%) remained

95 On confluence, cells on AM were continuously subcultured for six passages on AM or plastic.

96 entry did not require routine entry or exit subcultures for either system.

97 sufficient proliferative activity to permit subculturing for at least 2 passages.

98 t Staphylococcus aureus (MRSA) directly upon subculture from positive blood culture bottles.

99 Cultures of 3 species were subcultured from 3 to 5 times, with an average of 5.99 p

100 Unstimulated microglial cells, subcultured from an astrocyte coculture, typically exhib

101 in use, two (the E (Edinburgh) and J (Japan, subcultured from E)) are readily expelled by C57BL/6 mic

102 ation of the retinoblastoma protein in cells subcultured from the 60-day confluent control, AdBgl II-

103 to five different colonial morphologies were subcultured from the doxycycline medium, identified to s

104 After IRB approval, bacterial subcultures from 30 pediatric surgical patients with PA

105 sentangle specific transformation reactions, subcultures from active anaerobic microcosms were enrich

106 We report that subcultures from lines of female human embryonic stem ce

107 ioral characteristic detected in low density subcultures from the confluent cultures, and it persists

108 isolates of Salmonella serovar Typhi, before subculture, from other regions where Vi-negative Salmone

109 ) in 206 LIM enrichment broths by the use of subculture, GBS peptide nucleic acid fluorescent in situ

110 The improved growth after subculture greatly enhanced the reliability of limit-dil

111 Consistent data were obtained from subcultures grown for 3-day and 6-day periods, from the

112 Viability of B. burgdorferi was assessed by subculture, growth, morphology, and pH (as a surrogate f

113 bcultured bacteria or by treating repeatedly subcultured H. pylori with flurofamide (1 microM), a pot

114 ly 58 causal conjectures without controlling subculture homogeneity in SW480 cells.

115 Subculture homogeneity was controlled by real-time monit

116 ous SOX9 mRNA can be rapidly up-regulated in subcultured human articular chondrocytes if grown in alg

117 BRL 49653 treatment of subcultured human pre-adipocytes prepared from all depot

118 A representative of each was subcultured, identified to genus and species level, and

119 Additionally, organoid subcultures identify subclonal populations with altered

120 standard of care by eliminating the need for subculture.IMPORTANCERapid reporting of antimicrobial su

121 for toxin-producing E. coli O157:H7, and IMS subculture improved recovery.

122 n these cells were allowed to proliferate by subculture in DPI-free medium, an extensive G(1) delay w

123 ed at a much slower rate than controls after subculture in the absence of the drug, and required 9-12

124 lf-harm is associated with contemporary goth subculture in young people; however, whether this associ

125 y with full recovery of urease activity when subcultured in fresh microaerobic broth medium.

126 lysis of transcripts from human lymphoblasts subcultured in lipid-depleted sera (LDS) and LDS supplem

127 our hypothesis, S. flexneri strain 2457T was subcultured in media containing bile salts, and the abil

128 orbol myristate acetate or were passaged and subcultured in monolayer to induce dedifferentiation.

129 After initial pilot studies, they were subcultured in one of three groups: 1% porcine serum plu

130 When both sexual behaviors exist as subcultures in a population, disruptive selection can re

131 and E. faecalis grow normally over multiple subcultures in the absence of polyamines.

132 r transported in BHI followed by plating and subculturing in an enrichment medium, provides a simple

133 Frequent subcultures increased the number of unidentified isolate

134 ed their differential expression in parallel subcultures incubated with and without UVA.

135 epitope retain this level of expression when subcultured into broth.

136 when compared to the conventional AST, as no subculturing is needed and time to result is very short.

137 ontent could be obtained in under 6 h from a subcultured isolate, less time than traditional phenotyp

138 Controlled prior subculture led to the finding of a synergistic combinati

139 Subcultures made in the presence of FGF-4 had up to 10-f

140 opoiesis for several months, while untreated subcultures, made without FGF-4, grew erratically and ge

141 ied cultures have been grown for 1 year with subculturing, maintaining the introduced genes and pheno

142 n the high rate of false negatives, terminal subcultures may be helpful in certain situations.

143 atories have shown that during isolation and subculturing mesenchymal stem cells quickly lose the exp

144 gest that young people identifying with goth subculture might be at an increased risk for depression

145 is population by using a chromogenic medium, subculturing, molecular typing, and antifungal susceptib

146 Haploid yeast tel1 mec1 strains were subcultured nonselectively for approximately 200 cell di

147 Terminal subculture of "negative" bottles demonstrated viable yea

148 s, all BacT aerobic bottles, and by terminal subculture of all bottles.

149 analytical accuracy in comparison to routine subculture of blood culture bottles and phenotypic ident

150 Terminal subculture of bottles without detected growth recovered

151 visualization of pigment on day 1 or from a subculture of carrot broth.

152 included Gram staining of growth on BEAA and subculture of cocci on sheep blood agar plates for vanco

153 robial susceptibility testing (AST) involves subculture of organisms from positive blood bottles foll

154 Primary cell isolation and subculture of PT cells recapitulated HNF4A-associated lo

155 A subculture of students at each university form social bo

156 aecalis, and 1 Enterobacter sp.) on terminal subculture of the AER bottles when the companion PEDS PL

157 pported by exogenous FGF-2 and eliminated by subculture of the cells in presence of serum.

158 In addition, a subculture of the isolate was tested by a microdilution

159 the cell cycle-inhibitory proteins following subculture of the LTC cultures.

160 -negative cultures were detected by terminal subculture of the PEDS PLUS bottles when the companion A

161 On detailed examination, subcultures of 25 of the 32 VRE isolates produced two di

162 It also occurs in serial subcultures of cells that had been held under the constr

163 at XCI status should be routinely checked in subcultures of female hESCs, with cultures displaying XC

164 ion of BMPR expression was also evaluated in subcultures of human pulmonary arteriolar endothelial ce

165 ed on both media, and 12 were recovered from subcultures of SBM only.

166 ry isolation plate without the need for pure subcultures of suspect bacteria.

167 Incubation temperatures and subcultures of the media did not alter the rate of ident

168 Subcultures of the NSFCs have been passaged nearly 200 t

169 Repeated subculturing of AB22 resulted in improved growth and los

170 Extensive anaerobic subculturing of human feces identified no single commens

171 real-time PCR detection following successive subculturing of the bacterial isolate.

172 Repeated subculturing of Xanthobacter strain Py2 under nonselecti

173 ing modified 7H9 broth (8 weeks) followed by subculture on HEYM slants (monitored up to 8 weeks), and

174 specimens underwent routine processing with subculture on Mycoplasma-specific Hayflick agar.

175 of the myofibroblast phenotype using either subculture on soft substrates or TGF-beta receptor inhib

176 nantly gram-positive cocci in clusters) were subcultured on 5% sheep blood agar plates.

177 Primary rabbit endothelial cells were subcultured on 96-well plates at densities ranging from

178 ted to be senescent, since they could not be subcultured on agar medium.

179 tic morphology and keratocan expression when subcultured on AM stromal matrix even in the presence of

180 plastic was downregulated without CD34 when subcultured on AM.

181 ition to standard laboratory procedures were subcultured on blood agar plates for MALDI-TOF analysis.

182 ucrose medium, and presumptive colonies were subcultured on Gelatin Agar medium.

183 agenase-isolated keratocytes were seeded and subcultured on plastic or amniotic membrane matrix (AM)

184 A expression was upregulated when cells were subcultured on plastic.

185 d negative, the Gram stain was negative, and subcultures on chocolate agar and from the Isolator tube

186 and subsequent Gram stains were positive and subcultures on chocolate agar grew bacteria.

187 Subcultures on Lowenstein-Jensen agar confirmed the viab

188 Through serial-subcultures on xylose, we isolated evolved strains which

189 re incubated an additional 24 h for a second subculture, only 1 of 224 tests (0.4%) remained discrepa

190 Primary subculture onto Middlebrook 7H10, however, revealed thre

191 At 2 weeks, all plates were subcultured onto a fresh medium.

192 SBM cultures were subcultured onto blood agar and CNA agar plates, and the

193 ml, aliquots of catheter-exposed broth were subcultured onto blood agar at 15-min intervals.

194 h of the enrichment techniques, samples were subcultured onto modified semisolid Rappaport-Vassiliadi

195 Yeast isolates were subcultured onto Sabouraud dextrose agar and were incuba

196 Fecal specimens are subcultured onto selective and nonselective media, inclu

197 le functional properties following extensive subculture or differentiation into myofibroblasts and re

198 nge in MICs was noted following 5, 10, or 20 subcultures or at up to 6 months of frozen storage.

199 ce was stable in vitro after three rounds of subculture over 48 hours of growth in the absence of ant

200 (128 to 173 x 10(-8)) after 2 and 6 days of subculture (P < .001 overall).

201 The UPE of the NSC in the sixth subculturing passage was significantly higher than in th

202 stable in 10 strains following 10 sequential subculture passages.

203 -positive colonies from the direct and broth subculture plates were evaluated for the presence of VRE

204 24 tests (92%) were reproducible at the 24-h subculture point (94% for the SBT assay and 91% for the

205 c marker for BAT, in isolated adipocytes and subcultured preadipocytes prepared from different adult

206 associated antigen were observed in older or subcultured preparations concomitantly with the appearan

207 pB was associated with the outer membrane in subcultured preparations of H. pylori.

208 Using a recently described subculturing protocol to "induce" or accelerate EPEC adh

209 in neurofibroma formation and, by selective subculture, provide a resource for the development of an

210 ability within a laboratory occurred between subcultures rather than within gels or between gels.

211 lux was reduced sequentially with the fungal subculturing, rationalizing the decreasing on Taxol and

212 These strains could be subcultured repeatedly and retained capacity for differe

213 However, the repeated subcultures required to isolate sensitive variants can l

214 m the cultures after growth of 5, 10, and 15 subcultures, respectively.

215 Serial subculture resulted in a gradual increase in growth rate

216 Controlled homogeneous subculture resulted in a perturbation network of 321 cau

217 These subcultures retained the capacity to support hematopoies

218 of A. terreus conidial pigmentation with the subculturing, revealing the biosynthetic correlation of

219 colonies appearing on selective plates were subcultured, serotyped by the Quellung reaction, and gen

220 Upon subculture, stem cells formed colonies until passage 6 a

221 The subcultured strains had very high rates of chromosome ab

222 f freshly isolated corneal stromal cells and subcultured stromal fibroblasts from rabbits was used fo

223 etent to activate NF-kappaB in comparison to subcultured stromal fibroblasts.

224 act agar during the initial isolation and/or subcultures than they did on sheep blood or chocolate ag

225 lts between PCR and culture were resolved by subculturing the enrichment broth.

226 At the 10(th) subculture, the yield of Taxol was reduced by four folds

227 However, upon extensive subculturing, the level of exotoxin A produced by the PA

228 y difficult to grow as primary cultures, and subculturing these cells has been virtually impossible u

229 When subcultured, they began to grow and showed increased upt

230 crograms of colistin per ml (LIM broth) with subculture to another BAP and the costs associated with

231 nada agar to a Todd-Hewitt broth method with subculture to blood agar in order to determine which GBS

232 there was no growth of bacteria or yeasts on subculture to chocolate agar.

233 Carrot broth-enhanced subculture to GBS Detect (Hardy Diagnostics, Santa Maria

234 ple, the use of broth enrichment followed by subculture to MS offered a low-cost but sensitive method

235 without broth enrichment followed by a 24-h subculture to MS, was performed.

236 RSA (CM) and overnight broth enrichment with subculture to MSA (broth).

237 ctum in a selective broth medium followed by subculture to solid media and identification of GBS on t

238 broth containing 1 mg/liter of meropenem and subcultured to a MacConkey agar plate with a 10-mug mero

239 After 1 to 2 days of incubation, broths were subcultured to BEA VAN6 microg/ml agar.

240 ) PCR profiles, six species of bacteria were subcultured to blood agar plates and DNA was extracted f

241 ks, MD) positive by gram stain for yeast was subcultured to CHROMagar Candida (BD), and a 25-microg f

242 les flagged positive by the instruments were subcultured to determine both true-positive (growth) and

243 In addition, the same species were subcultured to fresh blood plates daily and DNA was extr

244 Positive samples were subcultured to isolate organisms harboring ESBL or carba

245 The differentiating culture can be subcultured to produce large amounts of myogenic progeni

246 sor mutations occurred frequently in stunted subcultures to recover growth rate.

247 tially associate with others who share their subculture, tool-using dolphins prefer others like thems

248 onditioned medium that allows us to grow and subculture total mouse keratinocytes for up to 19 subcul

249 mitans JP2-12 grew to high cell density when subcultured under iron-replete conditions.

250 ') appear in approximately 4 weeks; they are subcultured until adequate material is obtained for anal

251 DeltaNp63alpha or empty vector and serially subcultured until replicative senescence.

252 d eight plexiform neurofibromas by selective subculture using glial growth factor-2 and laminin.

253 dal concentrations (MFCs) were determined by subculturing visibly clear wells from the MIC microtiter

254 amined the effects loss-of-PDE1A function on subcultured VSMC growth and survival using PDE1A RNA int

255 We further demonstrated that in subcultured VSMCs redifferentiated by growth on collagen

256 , to assess false-negative bottles, terminal subcultures were done on all negative companion bottles

257 (MCF10A) with C/EBPbeta-2 virus, transformed subcultures were readily generated.

258 All solid media, including subcultures, were incubated for 8 weeks, providing a tot

259 akes at least 24 h to complete after isolate subculture, whereas hVISA is not routinely detected in c

260 l fast disease in mice, even after extensive subculture, whereas SY-infected cells produced only slow

261 ance, particularly if based on single-colony subcultures, will likely underestimate transmission even

262 Following sorting and subculture with MSCs, this CD34(+)CD41(low) population w

263 KSPG secretion was lost during serial subculture with or without FGF-2.

264 By repeated subculturing with increasing vancomycin (VAN) and cefuro

265 f the drug, and required 9-12 days of serial subculture, with selective growth of the faster growing

266 Brain Heart Infusion agar was achieved upon subculture without host cells.

267 nonsusceptibility was tested using repeated subculture without selective pressure.

268 Gram stain can impart, and in less time than subculturing, would allow the use of more directed empir

269 In summary, subculturing yeast directly from blood cultures onto CHR

270 cterium bovis that was isolated after serial subcultures, yet the functional basis for this attenuati