ライフサイエンスコーパス: sulfhydryl group

1 ent product results upon modification of the sulfhydryl group.

2 he creation of prodrugs that mask the labile sulfhydryl group.

3 om an ionizable donor, presumably the C(419) sulfhydryl group.

4 ddition or subtraction of a hydrogen atom or sulfhydryl group.

5 nt of an NO moiety to a nucleophilic protein sulfhydryl group.

6 al cross linking to a gold surface through a sulfhydryl group.

7 lve a reversible 1,4-addition with a protein sulfhydryl group.

8 fluorescent probe (monobromobimane) to label sulfhydryl groups.

9 isulfide bridges with cysteine or methionine sulfhydryl groups.

10 following hydrogen-bond classes for cysteine sulfhydryl groups.

11 maleimide, reagents that can modify cysteine sulfhydryl groups.

12 concentration-dependent oxidation of protein sulfhydryl groups.

13 rapped intermediates via cyanylation of free sulfhydryl groups.

14 two-cysteine residues and contained two free sulfhydryl groups.

15 the dynamic chemistry played by wine protein sulfhydryl groups.

16 achieved by a coupled reaction with protein sulfhydryl groups.

17 decrease in the availability of lipoic acid sulfhydryl groups.

18 sured were the levels of prolidase and total sulfhydryl groups.

19 compounds are nonpeptidic and do not contain sulfhydryl groups.

20 thyl methanethiosulfonate to block unreacted sulfhydryl groups.

21 ram, is a selective carbamoylating agent for sulfhydryl groups.

22 dified by a mechanism altering the status of sulfhydryl groups.

23 e reducing agent dithiothreitol restored the sulfhydryl groups.

24 ashed toenails, corresponding to As bound to sulfhydryl groups.

25 ma (CP) treatment led to a reduction in free sulfhydryl groups.

26 in surface hydrophobicity and decreased free sulfhydryl groups.

27 secondary structure, and increased the free sulfhydryl groups.

28 of Ag(+) and/or Cd(+2) with the substituted sulfhydryl groups.

29 ated As was present as trivalent As bound by sulfhydryl groups.

30 x1, which was mainly fully reduced with five sulfhydryl groups.

31 fluorescence detection of peptides with free sulfhydryl groups.

32 proteins and other ligands to silica through sulfhydryl groups.

33 be in the protection of superoxide-sensitive sulfhydryl groups.

34 e (GSH) is the major source of intracellular sulfhydryl groups.

35 oes not occur through direct inactivation of sulfhydryl groups.

36 of salvinorin A and an analogue with a free sulfhydryl group, 2-thiosalvinorin B.

37 We found that agents that disrupt free sulfhydryl groups abolish AP-1 binding activity in nucle

38 Indeed, a decrease of alpha-helix, free sulfhydryl groups amount and an increase of disulfide bo

39 he spontaneous reaction between the cysteine sulfhydryl group and highly reactive acrylyl-CoA, an int

40 s as well as gluten proteins; it removes the sulfhydryl group and leads to the formation of disulfide

41 res higher than 200 MPa, a decrease in total sulfhydryl groups and an increase in surface hydrophobic

42 ary lipid oxidation products and the loss in sulfhydryl groups and Ca(2+)-ATPase activity.

43 ified the GR protein by decreasing available sulfhydryl groups and decreasing nuclear GR expression a

44 It triggers a rapid covalent destruction of sulfhydryl groups and disulfide bonds via the mechanism

45 Dithiothreitol treatment restored the sulfhydryl groups and enzyme activity.

46 esults, obtained from the reactivity of free sulfhydryl groups and fluorescence analysis, showed that

47 Washed protein pellets were assayed for free sulfhydryl groups and for mixed protein disulfides with

48 r weight while significantly increasing free sulfhydryl groups and free amino group content (p < 0.05

49 Nonprotein sulfhydryl groups and glutathione reductase were not aff

50 Decay involves the increase in exposure of sulfhydryl groups and hydrophobic areas.

51 tein carbonyl content, oxidised amino acids, sulfhydryl groups and immuno-blotting against carbonyl g

52 nate (MTSES), used to irreversibly bind free sulfhydryl groups and inactivate the redox site, abolish

53 ocess, a recombinant E-cadherin lacking free sulfhydryl groups and its mutants with novel cysteines w

54 The level of accessible free sulfhydryl groups and the surface hydrophobicity of unfo

55 arsenite; and d) As has a high affinity for sulfhydryl groups and therefore binds to GSH and Cys.

56 peroxide, and phenylhydrazine) to cross-link sulfhydryl groups and with one fixative reagent (glutara

57 ), has no other active redox moieties (e.g., sulfhydryl groups) and can exist in three different oxid

58 y Bucillamine (BUC; an antioxidant drug with sulfhydryl groups) and Diltiazem (DLT, L-type calcium ch

59 ity on SDS-polyacrylamide gels, lack of free sulfhydryl groups, and atypical circular dichroism spect

60 the essential amino acids, carbonyl content, sulfhydryl groups, and functional properties of MLPC.

61 Nature forms S-S bonds by oxidizing two sulfhydryl groups, and no enzyme installing an intact hy

62 ivation involves the oxidation of ER-iPLA(2) sulfhydryl groups, and that ER-iPLA(2) inhibition increa

63 and Msh2-Msh6 interactions involve cysteine sulfhydryl groups, and the high Cd(2+):Msh2-Msh6 ratio i

64 As isolated, this sulfhydryl group appears to be in a disulfide linkage wi

65 ion was accompanied by the appearance of new sulfhydryl groups as determined with 5, 5'-dithiobis(2-n

66 m of alpha-lipoic acid), which contains free sulfhydryl groups as NAC does, decreased Ang II receptor

67 ducted to determine the contribution of free sulfhydryl groups as well as the disulfide bonds in alle

68 Hyperreactive sulfhydryl groups associated with the Ca(2+) release pro

69 disulfide trapping reflects the proximity of sulfhydryl groups, assuming similar accessibility and fl

70 d Hb As, Hb A-IAA and Hb A-NEM, in which the sulfhydryl group at beta93Cys is modified by sulfhydryl

71 jor site of HSA adduction is the single free sulfhydryl group at Cys34, we used thiol-affinity resins

72 182 of cyt b or 46 of the Fe-S protein or a sulfhydryl group at position 46 of cyt c1.

73 of single stranded DNA (ssDNA) probe through sulfhydryl group at the 5' phosphate end.

74 signal, suggesting the involvement of a free sulfhydryl group at this location in receptor-ligand int

75 The sulfhydryl groups at the end of the molecular wire form

76 ate to cysteine, most likely indicating that sulfhydryl groups at these positions are appropriately s

77 Of particular interest is Cys(95), whose sulfhydryl group becomes exposed to modification in the

78 e nitrosonium ion, transnitrosylating a free sulfhydryl group belonging to a cysteine residue tentati

79 ctional preinitiation complexes by modifying sulfhydryl groups but did not have any significant effec

80 gest that post-translational modification of sulfhydryl groups by a nitrogen monoxide (likely to be N

81 roperties except for their ability to modify sulfhydryl groups by oxidation, reduction, or alkylation

82 double mutant proves that mere loss of free sulfhydryl groups by oxidative regulation cannot account

83 ns that favored closure, indicating that the sulfhydryl groups come close enough to each other or to

84 subpopulation of alphaIIbbeta3 contains free sulfhydryl groups, consistent with the possibility that

85 on) and indirect (surface hydrophobicity and sulfhydryl group content) measurements.

86 The total sulfhydryl groups content was lower in the group DL + BA

87 edimentation, gluten index and negatively to sulfhydryl-groups content.

88 ionized selenol group in place of a cysteine sulfhydryl group could render this mammalian selenocyste

89 ition of NF-kappaB activity, suggesting that sulfhydryl groups critical for NF-kappaB activation were

90 Two sulfhydryl groups (Cys284 and Cys373) became inaccessibl

91 nd B2 remained unchanged, while free exposed sulfhydryl groups decreased.

92 pha-lipoic acid, which does not contain free sulfhydryl groups, did not.

93 Levels of sulfhydryl groups, dominated mainly by the high levels o

94 sulfur into homocysteine, which serves as a sulfhydryl group donor in the synthesis of cysteine by r

95 ealed that this mutation made the regulatory sulfhydryl group energetically much more difficult to re

96 ogical effects through reaction with exposed sulfhydryl groups, especially pairs of adjacent thiols.

97 These results indicate that the introduced sulfhydryl groups face the conduction pore and suggest t

98 o-ACP, forming the ACP analog that lacks the sulfhydryl group for the attachment of acyl chains for f

99 ifferent active sites and provide a terminal sulfhydryl group for the attachment of acyl groups throu

100 This sulfhydryl group forms a charge relay system with Asp-26

101 ppears to be in a disulfide linkage with the sulfhydryl group from the phosphopantetheine moiety of t

102 creasing pH and indicated that the pK of the sulfhydryl group had a pK value of 7.3.

103 ugh the reversible oxidation of key cysteine sulfhydryl groups has been demonstrated to occur in vitr

104 Nitrosylation of protein sulfhydryl groups has been shown to confer nitric oxide

105 ide (NEM), which reacts readily with exposed sulfhydryl groups, has been shown to inhibit the activit

106 Fab fragment, Fab fragment containing a free sulfhydryl group (i.e., Fab-SH), and whole antibody PZs

107 e protein, thioredoxin, between two cysteine sulfhydryl groups (i.e., staple), followed by photochemi

108 as the assay of reactivity of the introduced sulfhydryl group identify candidate positions that are i

109 tain a more stable form for oral dosing, the sulfhydryl group in conjugate 1 was converted into a fun

110 -linker that specifically cross-links a free sulfhydryl group in one protein (DrrB) to an amine in an

111 vity revealed that the functionally critical sulfhydryl group in the flippase protein is buried in a

112 le hydrogen-bonded states of buried cysteine sulfhydryl groups in a native protein.

113 The presence of free sulfhydryl groups in five recombinant monoclonal antibod

114 duction-oxidation modifications of cysteinyl sulfhydryl groups in mature ADAM17 may serve as a mechan

115 ct of CAPE, it suggests the role of critical sulfhydryl groups in NF-kappa B activation.

116 ent a method for identifying spatially close sulfhydryl groups in proteins employing chemical cross-l

117 can be used to quantify reactions targeting sulfhydryl groups in proteins.

118 the redox state of the insulin receptor (IR) sulfhydryl groups in response to reduced glutathione (GS

119 We proved the absence of accessible sulfhydryl groups in the oxidized product using liquid c

120 This procedure permits reaction of sulfhydryl groups in the periplasmic space with MPB, but

121 The amount of titrated sulfhydryl groups in the protein concentration range inv

122 GSH maintains protein sulfhydryl groups in the reduced form and has been impli

123 the reductase activity is unique in that no sulfhydryl groups in the YfcG protein are covalently inv

124 rease in carbonyl content and a reduction in sulfhydryl groups in their retinas.

125 theless, no derivatization strategy for free sulfhydryl groups in tissue is known for MALDI MSI.

126 enzymes that generate a product with a free sulfhydryl group, including histone acetyltransferases,

127 Free sulfhydryl groups increased during germination and in vi

128 ion, but without increased reactivity of its sulfhydryl groups, indicating that active-site alteratio

129 nges, specific for thiamine and sensitive to sulfhydryl group inhibition.

130 Na+ -H+ exchange inhibitor amiloride and the sulfhydryl group inhibitor p-chloromercuriphenylsulphona

131 nism of inhibition with N-ethyl maleimide, a sulfhydryl group inhibitor, indicates that this inhibiti

132 ifunctional protein cross-linker targeted to sulfhydryl groups, introduces intra-tetrameric cross-lin

133 ine-persulfide (Cys-SSH) is a cysteine whose sulfhydryl group is covalently bound to sulfur (sulfane

134 arine by reducing agents suggests a critical sulfhydryl group is involved in NF-kappaB activation.

135 ing properties, indicating that the reactive sulfhydryl group is located within a transmembrane domai

136 lation by sulfhydryl reagents, this reactive sulfhydryl group is on Cys-199 of the receptor.

137 sted that the presence of low levels of free sulfhydryl groups is likely a common feature of recombin

138 This further ensured that any sulfhydryl groups liberated during the digestion would b

139 ently interact with a class of hyperreactive sulfhydryl groups localized on ryanodine-sensitive Ca2+

140 yltriglycine with S-acetyl protection of the sulfhydryl group may be used to conjugate MAG3 to primar

141 Because spontaneous modification of sulfhydryl groups may also occur in vivo, tumor vaccines

142 er yellow iodoacetamide (LYI), suggests that sulfhydryl groups might play a role in the catalytic mec

143 s a T-rich probe DNA at one vertex and three sulfhydryl groups modified with 10nm Au NPs at the other

144 e of the enzyme and that AN was sensitive to sulfhydryl-group-modifying reagents in general.

145 sence of 1 mM MgADP revealed nearly two free sulfhydryl groups/mol of enzyme.

146 Besides the sulfhydryl group of a cysteine, we make use of an azido

147 f the C-terminal amino acid is linked to the sulfhydryl group of a cysteine, which is always the fift

148 ences of chemically modifying the Cys beta93 sulfhydryl group of both the deoxy and CO-saturated deri

149 lfide exchange between the inhibitor and the sulfhydryl group of C159.

150 inhibitor of the enzyme, suggesting that the sulfhydryl group of CoA is important for the optimizatio

151 es and that oxidized Cys(48) reacts with the sulfhydryl group of Cys(152) to form an intramolecular d

152 ally replaced with serine suggested that the sulfhydryl group of Cys(48) is the site of oxidation by

153 luding His-146 and Glu-172 that activate the sulfhydryl group of Cys-145 to allow its nucleophilic at

154 minal carbon of the acetylene moiety and the sulfhydryl group of Cys-803 at the solvent interface.

155 The reactive sulfhydryl group of Cys106 projects toward position C-4a

156 e presence of monovalent copper ions and the sulfhydryl group of Cys466.

157 yphenols (o-quinones) react rapidly with the sulfhydryl group of cysteine (Cys) residues, inducing co

158 e a counter ion to promote ionization of the sulfhydryl group of GSH, thereby accounting for the obse

159 olution containing NEM which reacts with the sulfhydryl group of GSH, thus locking the active form in

160 process, involves reaction of the side chain sulfhydryl group of homocysteine with its activated carb

161 nent of complement (C3), we coupled the free sulfhydryl group of methylamine-inactivated C3 to a thio

162 Whereas replacement of the sulfhydryl group of tetrapeptide CVLS (I50 = 1 microM) b

163 g peroxyl radical may react further with the sulfhydryl group of the C419 residue to form the sulfiny

164 confirmed by examining the reactivity of the sulfhydryl group of the Cysbeta93 toward 4, 4'-dipyridyl

165 ules were immobilized through the side-chain sulfhydryl group of the cysteine residue on several supp

166 disulfide interchange between an engineered sulfhydryl group of the protein (Q258C mutation) and a d

167 ilic attack of either the amine group or the sulfhydryl group of the substrate on the internal aldimi

168 te (APMA) work by directly reacting with the sulfhydryl group of this cysteine residue, resulting in

169 t-activated rhodopsin, the reactivity of the sulfhydryl groups of a constitutively active R175Q arres

170 The sulfhydryl groups of all mutants reacted rapidly with th

171 rategy was demonstrated by cross-linking the sulfhydryl groups of Cys-18 and Cys-78 in gamma-crystall

172 Reaction of MBB with the sulfhydryl groups of free cysteines leads to formation o

173 nge between thioredoxin f and the regulatory sulfhydryl groups of fructose-1,6-bisphosphatase, sedohe

174 g assay was designed to detect alkylation of sulfhydryl groups of human Keap1.

175 Arsenic binding by sulfhydryl groups of natural organic matter (NOM) was re

176 ecently, the complexation of trivalent As by sulfhydryl groups of NOM was proposed as the main mechan

177 Sulfhydryl groups of protein-cysteines constitute main t

178 Nitric oxide (NO) reacts with the sulfhydryl groups of proteins to form nitroso thiols.

179 xidant capacity (TAC), protein carbonyl, and sulfhydryl groups of the antioxidant system were analyze

180 be associated with its direct action on the sulfhydryl groups of the channel protein.

181 All three of the sulfhydryl groups of the mutant arrestin R175Q reacted r

182 The sulfhydryl groups of the three cysteines in bovine arres

183 redox midpoint potentials for the regulatory sulfhydryl groups of the various enzymes spanned a broad

184 The reactive sulfhydryl group on Cys beta93 in human adult hemoglobin

185 is-(2-nitrobenzoic acid), which modifies the sulfhydryl group on cysteines, caused irreversible disso

186 g that all three compounds acted at the same sulfhydryl group on the alternative oxidase protein.

187 hilic additions with reagents containing two sulfhydryl groups on adjacent carbon atoms to form a cyc

188 e the formation of adducts with nucleophilic sulfhydryl groups on cysteine residues of selected prote

189 hese findings, we conclude that oxidation of sulfhydryl groups on intracellular targets results in in

190 modification of 1 or more of the 27 cysteine sulfhydryl groups on Keap1 (a protein that sequesters Nr

191 The addition of acrylamide to sulfhydryl groups on proteins is modeled using glutathio

192 disulfide bonds between low m.w. thiols and sulfhydryl groups on specific cytosolic proteins (S-thio

193 tion resulted in cross-linking of the unique sulfhydryl groups on subunit B with other subunits in th

194 hat these methods had a high selectivity for sulfhydryl groups on this protein, which accounted for t

195 on in vitro and decreased the number of free sulfhydryl groups on tubulin cysteines.

196 periplasm causes noxious reactions, such as sulfhydryl group oxidation leading to enzymatic inactiva

197 reas the polar or chemical properties of the sulfhydryl group play only a minor role; 3) the function

198 cysteine, which adds to the cofactor via its sulfhydryl group, possibly forming a cyclic thiazolidine

199 ue containing a methyl group in place of the sulfhydryl group present in Cys, for the native Cys resi

200 in gold or self-assembled monolayer via the sulfhydryl groups present in the hinge region.

201 N-ethylmaleimide (NEM) was used to mask free sulfhydryl groups present in the mAb.

202 n of complexes involving mercury species and sulfhydryl groups present in tissues and/or loss of wate

203 ivity, and this activity could be blocked by sulfhydryl group-reactive inhibitors, N-ethylmaleimide a

204 The intrinsic reversible disulfide bond/sulfhydryl group reconfiguration of gluten networks is e

205 t protein damage, characterised by the total sulfhydryl group reduction.

206 of actin, IAM was used to block any residual sulfhydryl groups remaining on the protein so that they

207 pproach, a cemadotin derivative containing a sulfhydryl group results in a mixed disulfide linkage.

208 Two GSH analogues that lack a sulfhydryl group, S-hexylglutathione (SHG) and oxidized

209 se enzymes and as ionization of the Cys(184) sulfhydryl group seems required for sortase activity, we

210 strategy presents a rapid method for mapping sulfhydryl groups separated by a distance of approximate

211 Reversibly oxidized cysteine sulfhydryl groups serve as redox sensors or targets of r

212 is simulation that the distances between the sulfhydryl group (SG) of Cys81 to the C6 of Cyt18 (SG-C6

213 -isoascorbic acid (DIA), on the oxidation of sulfhydryl groups (-SH) to disulfide bonds (-SS-) of ext

214 -isoascorbic acid (DIA), on the oxidation of sulfhydryl groups (-SH) to disulfide bonds (-SS-) of ext

215 Binding of arsenite (As(III)) to sulfhydryl groups (Sorg(-II)) plays a key role in As det

216 lkylation of mature sublancin showed no free sulfhydryl groups, suggesting that one sulfydryl had for

217 Zeta potential, disulfide-sulfhydryl groups, surface hydrophobicity, secondary str

218 ain why cysteine, despite possessing a polar sulfhydryl group, tends to behave as a hydrophobic (rath

219 Rabbit Fab'-SH fragments contain a free sulfhydryl group that forms a thiolate bond with a gold

220 This protein has a reactive sulfhydryl group that is essential for activity.

221 ral of the culprit drugs contain one or more sulfhydryl groups that may have potential mechanistic re

222 t as antioxidant drugs directly through free sulfhydryl groups that serve as a source of reducing equ

223 sidue disulfide loop by selectively labeling sulfhydryl groups that were liberated only after chemica

224 only in those fragments that contained free sulfhydryl groups: the light chain (Cys-1960, Cys-2113),

225 Methyl acrylate reacted instantly with the sulfhydryl group to form a derivative that was stable in

226 to the terminal carbon of homoserine via its sulfhydryl group to form cystathionine, which is cleaved

227 oyl cofactor, which is the attachment of two sulfhydryl groups to C6 and C8 of a pendant octanoyl cha

228 nd the use of a molecular wire terminated in sulfhydryl groups to connect the two modules.

229 the selective oxidation of protein cysteine sulfhydryl groups to disulfide bonds we examined the spe

230 The oxidation of sulfhydryl groups to disulfides as another possible sour

231 chicken egg white catalyzes the oxidation of sulfhydryl groups to disulfides with reduction of oxygen

232 chicken egg white catalyzes the oxidation of sulfhydryl groups to disulfides with the reduction of ox

233 de of cysteine residues after cyanylation of sulfhydryl groups to form an amino-terminal peptide and

234 trosylhemoglobin, NO can react with cysteine sulfhydryl groups to form S-nitrosocysteine or cysteine

235 by analyzing accessibility of the introduced sulfhydryl groups to thiophilic Cd(2+).

236 ioredoxin and inhibits its ability to reduce sulfhydryl groups via NADPH oxidation.

237 lycol-conjugated maleimide of 5 kDa for each sulfhydryl group, we find that PDI reduction and oxidati

238 ng advantage of the reactivity of cysteine's sulfhydryl group, we modified these mutants with chemica

239 capacity against peroxyl radicals, DPPH, and sulfhydryl groups were analyzed.

240 The locations of the free sulfhydryl groups were determined using mass spectrometr

241 plasmin activity, prolidase level, and total sulfhydryl groups were evaluated.

242 Free sulfhydryl groups were first modified using 5-idoacetami

243 tially reduced sillucin species; the nascent sulfhydryl groups were immediately cyanylated by 1-cyano

244 This protection does not extend to the mAAT sulfhydryl groups which in the GroEL-mAAT complex have s

245 Modification of the introduced sulfhydryl group with membrane-impermeant methanethiosul

246 Blocking the beta-93 sulfhydryl group with N-ethylmaleimide was found to elim

247 reitol and was blocked by modification of Ng sulfhydryl groups with 4-vinylpyridine.

248 Titration of protein sulfhydryl groups with 5,5'-dithiobis(2-nitrobenzoate) (

249 and enhanced reactivity of one of arrestin's sulfhydryl groups with inhibition of the reactivity of a

250 s with dithiothreitol and alkylated the free sulfhydryl groups with iodoacetamide.

251 Redox-dependent modifications of sulfhydryl groups within the two Cys4 zinc fingers of th