ライフサイエンスコーパス: sulfotyrosine

1 ucing specific intermediates with one or two sulfotyrosines.

2 ecific intermediates with one, two, or three sulfotyrosines.

3 ligand-receptor interactions and identifies sulfotyrosine 174 as the critical C3a docking site.

4 t on the CXCL12 surface normally occupied by sulfotyrosine 21 (sY21), and five were selected for expe

5 tide stabilizes dimeric SDF-1alpha, and that sulfotyrosine 21 binds a specific site on the chemokine

6 xtreme N-terminal region of CXCR4, including sulfotyrosine 21, make specific contacts with the chemok

7 s containing unmodified tyrosine (160-fold), sulfotyrosine (3600-fold), phosphotyrosine (>8000-fold),

8 Although the vGPCR variant lacking sulfotyrosines activated downstream signaling pathways,

9 idues in V(H) CDR3 were randomized, contains sulfotyrosine and binds gp120 more effectively than a si

10 gonal aminoacyl-tRNA synthetase specific for sulfotyrosine and its cognate orthogonal tRNA that recog

11 GSO1/SGN3 harbors a binding pocket for sulfotyrosine and makes extended backbone interactions w

12 Sulfotyrosine and other negatively charged residues in t

13 d with wild-type sperm treated with the anti-sulfotyrosine antibody PSG2.

14 e, along with a simple chemical synthesis of sulfotyrosine, are outlined in this protocol.

15 a classical signaling GPCR, with N-terminal sulfotyrosines as the only signaling and chemokine-bindi

16 e 302 of SBT1.8 was found to be required for sulfotyrosine binding and for accurate processing of the

17 leukotoxins and suggests that the conserved sulfotyrosine binding pocket could be a target of choice

18 sequences revealed the location of receptor sulfotyrosine binding sites in the toxins.

19 further demonstrated that the binding of the sulfotyrosine-binding pocket by CCR5mim2-Ig was sufficie

20 on and illustrate the utility of binding the sulfotyrosine-binding pockets of gp120.

21 eptor associated with Nt peptides containing sulfotyrosines but not with peptides containing sulfotyr

22 f the complex between the CXCL12 dimer and a sulfotyrosine-containing CXCR4 fragment enabled high-thr

23 phosphorylation and vGPCR tumorigenesis in a sulfotyrosine-dependent manner.

24 onserved site on gp120, whose recognition of sulfotyrosine engenders posttranslational mimicry by the

25 Consistent with a functional role of the V2 sulfotyrosines, enhancement of tyrosine sulfation decrea

26 ynthetase mutant for the genetic encoding of sulfotyrosine in mammalian cells.

27 and draw attention to the important role of sulfotyrosine in mediating receptor-ligand interactions.

28 genetic code that co-translationally inserts sulfotyrosine in response to the amber nonsense codon, T

29 In addition to sulfotyrosines in positions 10 and 14, negatively charge

30 responding to CCR5 Nt residues 2 to 18, with sulfotyrosines in positions 10 and 14.

31 fotyrosines but not with peptides containing sulfotyrosines in scrambled Nt sequences.

32 Acidic residues and sulfotyrosines in the amino-terminal domain (Nt) of CCR5

33 Finally, only peptides containing sulfotyrosines inhibited the entry of an R5 isolate.

34 ere we report the selective incorporation of sulfotyrosine into proteins in bacteria by genetically e

35 The presence of sulfotyrosine is indicated by the detection of free tyro

36 er conditions in which the sulfuryl group of sulfotyrosine is labile.

37 tivation triggered by chemokine agonists via sulfotyrosines is necessary for vGPCR tumorigenesis, the

38 cresyl sulfate) and with peptides containing sulfotyrosines issued from receptor sequences revealed t

39 These results identify the sulfotyrosine-mediated V2-V3 interaction as a critical c

40 We have developed a novel anti-sulfotyrosine monoclonal antibody (called PSG2) that bin

41 ing affinity chromatography on PSG2, an anti-sulfotyrosine monoclonal antibody, followed by mass spec

42 Nonetheless, a critical sulfotyrosine on CCR5 and on 412d induces similar struct

43 ms the fluorosulfated tyrosine peptides into sulfotyrosine peptides in high yield.

44 y of CXCR4 1-38 increases with the number of sulfotyrosines present, which suggests a potential physi

45 Our results suggest that sulfotyrosine recognition sites can be targeted for the

46 ing two other binding pockets that recognize sulfotyrosine residues (sY12 and sY21) of CXCR4, includi

47 h high affinity and exquisite specificity to sulfotyrosine residues in peptides and proteins independ

48 uch that they would be able to interact with sulfotyrosine residues on C4.

49 lasma-stable d-cyclic peptide bearing four d-sulfotyrosine residues, exhibited nanomolar affinity for

50 acts with a peptide from C4 containing three sulfotyrosine residues.

51 he anion-binding exosites of the enzymes via sulfotyrosine residues.

52 zymes, leading to a final product with three sulfotyrosine residues.

53 enzymes leading to a final product with four sulfotyrosine residues.

54 th therapeutic potential contain one or more sulfotyrosine residues.

55 E in complex with a small molecule mimicking sulfotyrosine side chain (p-cresyl sulfate) and with pep

56 e autoantibodies, we incorporated pNO(2)Phe, sulfotyrosine (SO(3)Tyr), and 3-nitrotyrosine (3NO(2)Tyr

57 Anti-sulfotyrosine staining of sperm showed localization simi

58 g interactions in phosphotyrosine (pTyr) and sulfotyrosine (sTyr) residues.

59 ovides interaction sites with the hormone: a sulfotyrosine (sTyr) site in the hinge region consistent

60 lity to biosynthesize and genetically encode sulfotyrosine (sTyr), an important protein post-translat

61 f all l-amino acids, with reprogramming of l-sulfotyrosine to mimic the presence of this post-transla

62 We found that sulfotyrosine (Tys) 278 enhances the interaction mainly

63 oacyl-tRNA synthetase/tRNA pair specific for sulfotyrosine, we were able to determine the contributio