ライフサイエンスコーパス: synapsis

1 2 supports homologous chromosome pairing and synapsis.

2 t has no known role following Rad51-mediated synapsis.

3 d is most likely important for initiation of synapsis.

4 ed for meiotic recombination and chromosomal synapsis.

5 ut that their primary function is to trigger synapsis.

6 on of recombination, chromosome pairing, and synapsis.

7 alpha) segments that should facilitate their synapsis.

8 cumulate unrepaired DNA and fail to complete synapsis.

9 cal juxtaposition of DNA ends is called NHEJ synapsis.

10 rference-sensitive crossovers and chromosome synapsis.

11 tromeres is the initiating event for meiotic synapsis.

12 the nuclear envelope needed for pairing and synapsis.

13 nduce NE remodeling, chromosome pairing, and synapsis.

14 promote PC complex aggregation, pairing, and synapsis.

15 tial PC pairing to ensure correct homologous synapsis.

16 o define the first step in the initiation of synapsis.

17 ly, without any obvious defect in chromosome synapsis.

18 aded with Zip1p in a manner that may promote synapsis.

19 t of models for the role of PCs in promoting synapsis.

20 ngle RSS that are paired in trans to promote synapsis.

21 re essential for both homologous pairing and synapsis.

22 Zip2 and Zip4 are dispensable for chromosome synapsis.

23 he Ku inward to expose the overhang for NHEJ synapsis.

24 defective DNA binding and no detectable DNA synapsis.

25 luding chromosome fragmentation, pairing and synapsis.

26 important and rate-limiting step to complete synapsis.

27 ermatocytes exhibit a failure in chromosomal synapsis.

28 ated mutants is slow relative to the rate of synapsis.

29 in the PAR simply by delaying or preventing synapsis.

30 ve Y324F and R173K mutants are defective for synapsis.

31 nters to ensure proper homologue pairing and synapsis.

32 omplexes remain stable but do not proceed to synapsis.

33 checkpoint pathway that monitors chromosome synapsis.

34 absence results in nonhomologous pairing and synapsis.

35 C) required for the regulation of chromosome synapsis.

36 -order nucleoprotein complexes necessary for synapsis.

37 -like factor (XLF), a critical factor in end synapsis.

38 in the presence or absence of GTP, prior to synapsis.

39 element ends in the absence of GTP prior to synapsis.

40 Y fuse through a dense plate without obvious synapsis.

41 mmobilized DNA fragments that cannot undergo synapsis.

42 -effect relationship between sumoylation and synapsis.

43 G proteins' ability to sense 12/23-regulated synapsis.

44 is due to a failure of homologous chromosome synapsis.

45 as chromosomes compact and homologs undergo synapsis.

46 NA ends are brought together - a step called synapsis.

47 moting protein Hop1/HORMAD during chromosome synapsis.

48 cluding a full DNA double-strand break (DSB) synapsis.

49 say where we can measure the duration of the synapsis.

50 cytes that are severely defective in homolog synapsis.

51 mation of DSBs and completion of pairing and synapsis.

52 omeres is a continuation of the interstitial synapsis.

53 ion, homologous recombination and chromosome synapsis.

54 ances the probability of success at fruitful synapsis.

55 e (RSS)-conserved regions before nicking and synapsis.

56 ate binding of Int to the P half site during synapsis.

57 nd BUB-3 require full PC function to inhibit synapsis.

58 required during immunoglobulin switch region synapsis.

59 affecting homologous chromosome pairing and synapsis.

60 t period of postreplicative sister chromatid synapsis, a situation that is more reminiscent of eukary

61 dies lead us to propose that SYP-3 regulates synapsis along chromosomes, contributing to meiotic prog

62 ic processes that included recombination and synapsis, along with gene sets involved in RNA metabolis

63 tudies confirmed that polymerase-induced end synapsis also occurs in solution.

64 In Caenorhabditis elegans, synapsis and a checkpoint that monitors synapsis depend

65 s in severe defects in homologous chromosome synapsis and an early-stage failure in meiotic recombina

66 t this can be circumvented by preventing Y-Y synapsis and associated Y gene expression.

67 The involvement of some residues in both synapsis and catalysis suggests that they contribute to

68 ns initiate V(D)J recombination by mediating synapsis and cleavage of two different antigen receptor

69 iotic double-strand break repair, chromosome synapsis and crossover numbers.

70 Effects on male meiotic chromosome synapsis and derepression of transposable elements (TEs)

71 BPA exposure results in impaired chromosome synapsis and disruption of meiotic double-strand break r

72 op2 in mice eliminates homologous chromosome synapsis and disrupts double-strand break (DSB) repair t

73 prior to formation of recombinants (binding, synapsis and DNA cleavage).

74 ng a cell-free system that recapitulates end synapsis and DNA-PKcs autophosphorylation, we found a de

75 ced by full-length RAG1, and associated with synapsis and efficient RSS cleavage.

76 , or tension, between homologues to regulate synapsis and elicit a checkpoint response.

77 While synapsis and events associated with class I crossovers a

78 activity is required for homolog pairing and synapsis and for double-strand break formation, but how

79 hese events when all homologue pairs achieve synapsis and form crossover precursors.

80 11 promotes initiation and/or maintenance of synapsis and formation of crossovers, and may provide a

81 r DNA double-strand breaks (DSBs), involving synapsis and ligation of the broken strands.

82 1c results in complete sterility, incomplete synapsis and meiotic chromosome fragmentation, suggestin

83 vel protein that is required for chromosomal synapsis and meiotic recombination.

84 ption of the Raptor gene impairs chromosomal synapsis and prevents the efficient spreading of silenci

85 cytes displayed normal homologous chromosome synapsis and progression through meiosis.

86 nd BUB-3 are required to negatively regulate synapsis and promote the synapsis checkpoint response.

87 Meiotic synapsis and recombination between homologs permits the

88 t the CDKG1/CYCLINL complex is essential for synapsis and recombination during male meiosis.

89 rovide new insights on the interplay between synapsis and recombination in barley and highlight the n

90 al dimer-dimer interactions are required for synapsis and recombination, using a novel complementatio

91 hromatin hub that supports V(alpha)-J(alpha) synapsis and recombination.

92 ring meiosis, homologous chromosomes undergo synapsis and recombination.

93 ar hallmarks of meiotic chromosome cohesion, synapsis and recombination.

94 ence of key features of meiosis I, including synapsis and recombination.

95 mechanism underlying its role in chromosome synapsis and recombination.

96 NA structures and Hop1 in meiotic chromosome synapsis and recombination.

97 cations of our results for the mechanisms of synapsis and regulation in recombination by wild-type re

98 roles in determining the sequential order of synapsis and remodeling before end joining.

99 ister chromatid cohesion and enables correct synapsis and segregation of homologous chromosomes durin

100 SYN3 caused defects in homologous chromosome synapsis and synaptonemal complex (SC) formation during

101 complex, exhibits a high level of chromosome synapsis and that most DSBs in these spermatocytes are r

102 y, a male-specific organelle associated with synapsis and the formation of the XY body during meiosis

103 atocytes are defective in meiotic chromosome synapsis and undergo apoptosis during Prophase I.

104 XYY males exhibit Y-Y synapsis and Y chromosomal escape from MSCI without acco

105 However, these also harbor defects in synapsis and/or recombination and as such may activate a

106 naptonemal complex (SC) assembly (chromosome synapsis), and crossover recombination are essential for

107 The reaction involves DNA binding, synapsis, and cleavage at two RSSs located on the same D

108 chromosomes during meiosis requires pairing, synapsis, and crossing-over.

109 d, leading to defects in DSB repair, homolog synapsis, and crossover formation.

110 some segregation requires homologue pairing, synapsis, and crossover recombination, which occur durin

111 -bound sites at DSB hotspots is critical for synapsis, and hence fertility.

112 fect key prophase processes such as pairing, synapsis, and homologous recombination.

113 re behavior, meiotic recombination, pairing, synapsis, and installation of the meiosis-specific cytos

114 plexes that facilitate DNA accumulation, DNA synapsis, and MMEJ.

115 on of chromosomal events, including pairing, synapsis, and recombination.

116 rgue that BLM is involved in proper pairing, synapsis, and segregation of homologous chromosomes; how

117 of the timing and progression of chromosome synapsis, and the gradual release of the individual cent

118 Chromosome synapsis appears to proceed normally, without significan

119 rstitial ZYP1 loci elongating at zygotene so synapsis at centromeres is a continuation of the interst

120 In many organisms, homolog pairing and synapsis at meiotic prophase depend on interactions betw

121 lacking SIX6OS1 are defective in chromosome synapsis at meiotic prophase I, which provokes an arrest

122 This structure facilitated S-S synapsis because Smicro was proximal to Emicro and a dow

123 normal prophase program of recombination and synapsis between homologous chromosomes, including loadi

124 ut does not require double-strand breaks for synapsis between homologous chromosomes.

125 ood but may have a critical role in ensuring synapsis between homologs and regulating double-strand b

126 meiosis in budding yeast and mammals is that synapsis between homologs depends upon recombination; ho

127 rrelates with the elongation of interstitial synapsis between the corresponding homologues.

128 rmation of the synaptonemal complex (SC), or synapsis, between homologs in meiosis is essential for c

129 rate-limiting step of excision occurs after synapsis, but closely precedes or is concomitant with th

130 Male Drosophila lack synapsis, but nonetheless, their chromosomes closely ass

131 The allosteric effect of GTP in promoting synapsis by P element transposase may be to orient a sec

132 Here we show that CHK-2 promotes pairing and synapsis by phosphorylating a family of zinc finger prot

133 Here, we directly observe the NHEJ synapsis by pol mu using a single molecule FRET (smFRET)

134 The promotion of close synapsis by XLF indicates a role that is independent of

135 ngs show that, like the end processing step, synapsis can be achieved through several mechanisms.

136 aired chromosome axes, and premature loss of synapsis checkpoint protein PCH-2.

137 negatively regulate synapsis and promote the synapsis checkpoint response.

138 tant assembles aberrant SC that triggers the synapsis checkpoint.

139 ggests that DSBs are being repaired in these synapsis-defective mutants.

140 The chromosome synapsis defects and Prophase I apoptosis of Pol beta-de

141 persistent DMC1 foci, severe DSB repair and synapsis defects, and downstream sterility.

142 ing is genetically separable from subsequent synapsis, defined as stabilization of pairing by the syn

143 ans, synapsis and a checkpoint that monitors synapsis depend on pairing centers (PCs), cis-acting loc

144 In C. elegans, homolog pairing and synapsis depend on pairing centers (PCs), special region

145 However, 12/23-regulated synapsis does not strongly stimulate the cleavage activi

146 mechanisms, induces directional movement and synapsis driven by the machinery responsible for recombi

147 morphological changes required for homologue synapsis, DSB repair, and meiotic chromosome segregation

148 architectural scaffolding that promotes S-S synapsis during CSR and that these interactions are stab

149 1 (CDKG1) is necessary for recombination and synapsis during male meiosis at high ambient temperature

150 lays an important role in initiating homolog synapsis during meiosis in Drosophila females.

151 t these sites mediate chromosome pairing and synapsis during meiosis, and that each site contains bin

152 hich are essential for homologous chromosome synapsis during meiosis.

153 completely disrupted chromosome pairing and synapsis during meiosis.

154 assembly that mediates homologous chromosome synapsis during meiosis.

155 nd this is associated with severe defects in synapsis during the first meiotic division and reduced m

156 Chromosome synapsis during zygotene is a prerequisite for the timel

157 As short stretches of subtelomeric synapsis emerged at early zygotene, centromere clusters

158 bing target-searching, integration, and post-synapsis events.

159 Telomere bouquet formation is normal, but synapsis fails and oocytes accumulate in large numbers a

160 tosis during midpachytene of male meiosis if synapsis fails.

161 nstrate its combination with FRET to observe synapsis formation by Cre using excitation by a single l

162 elomere bouquet followed by the extension of synapsis from telomeres at the base of the bouquet, thus

163 sence of GTP or nonhydrolyzable GTP analogs, synapsis happens rapidly, whereas DNA cleavage is slow.

164 s structurally predicted to be important for synapsis, have been generated and characterized.

165 ome X chromosomes achieve nonhomologous self-synapsis; however, germ cells with SYP-1-positive X chro

166 The observed level of synapsis implies that Ph1 functions to promote homologue

167 esion, is required for homologue pairing and synapsis in budding yeast.

168 is-specific protein essential for chromosome synapsis in budding yeast.

169 We investigated the genetic requirements for synapsis in Drosophila and found that there are three te

170 the Mre11 complex in meiotic DNA repair and synapsis in mammals and indicate that the complex may co

171 s revealed incomplete chromosome pairing and synapsis in meiotic prophase, and extensive chromosome f

172 bservations can be well explained by ectopic synapsis in NAHR together with our proposed model of chr

173 ybl1(repro9)) had subtle defects in autosome synapsis in pachynema, a high incidence of unsynapsed se

174 e-molecule FRET experiments that observe end synapsis in real-time show that this defect is due to a

175 chromosome fragmentation and the absence of synapsis in the initial stages of meiosis.

176 fy the protein-DNA interactions that lead to synapsis in the Tn5 system.

177 me axes and about the spatial progression of synapsis in three dimensions.

178 -6 mutants partially restores the defects in synapsis, in agreement with FKB-6 acting by decreasing c

179 among organisms with limited sex chromosome synapsis, including mammals.

180 e that the probability of ectopic chromosome synapsis increases with increased LCR length, and that e

181 nto distinct loop domains and inhibiting RSS synapsis, independent of any effects on transcription, R

182 budding yeast have suggested that chromosome synapsis initiates at the sites of crossing over.

183 Os and SYP-1 protein support models in which synapsis initiates predominantly in the vicinity of pair

184 ial associations, where proteins involved in synapsis initiation are located.

185 Surprisingly, synapsis initiation at centromeres is independent of the

186 Fpr3 and Zip3 appear to specifically prevent synapsis initiation at centromeric sites.

187 w that without Fpr3 and Zip3 activities, the synapsis initiation components Zip2 and Zip4 are dispens

188 Surprisingly, late zygotene synapsis initiation events are independent of the earlie

189 he Zip3 protein, which plays a major role in synapsis initiation events at noncentromeric locations.

190 d-zygotene events, whereas both mid and late synapsis initiation events depend on the cohesin subunit

191 Our data provide evidence for two classes of synapsis initiation events that differ in location, timi

192 eric chromatin coinciding with key events of synapsis initiation from the subtelomeric regions.

193 n in regions distant from prominent sites of synapsis initiation, and CO-inhibitory role(s) that limi

194 ormation, the roles of centromere pairing in synapsis initiation, and the mechanisms by which oocytes

195 emporally and genetically distinct stages of synapsis initiation.

196 centromeric regions are preferred sites for synapsis initiation.

197 segments, could potentially be mediated by a synapsis intermediate involving an intergenic parallel-s

198 se IV NHEJ ligation complex, that end-to-end synapsis involves a dynamic positioning of the two ends

199 with increased LCR length, and that ectopic synapsis is a necessary precursor to ectopic crossing-ov

200 aptic state, and maximum efficiency of close synapsis is achieved within 20 min.

201 Early end synapsis is associated with kinase autophosphorylation.

202 le for homologous pairing and continue until synapsis is completed.

203 TIVE51 (RAD51) foci are largely reduced, and synapsis is completely abolished in dsy2 meiocytes.

204 kpoint-like manner to ensure that chromosome synapsis is contingent on the initiation of recombinatio

205 ank and, unexpectedly, the RSS spacer, while synapsis is controlled primarily by the RSS nonamer.

206 In the cdkg1-1 mutant, synapsis is impaired and there is a dramatic reduction i

207 for meiotic chromosome segregation, but how synapsis is initiated between chromosomes is poorly unde

208 S-S synapsis is mediated by a chromatin loop that spans the

209 Synapsis is mediated solely by Polmu, facilitated by sin

210 one end of a linear stretch, suggesting that synapsis is often unidirectional.

211 In "early zygotene" oocytes, synapsis is only observed at the centromeres.

212 Homologue synapsis is required for meiotic chromosome segregation,

213 ted DNA cleavage before or immediately after synapsis is required to stabilize the synaptic assemblie

214 Synapsis is the process by which paired chromosome homol

215 ent to the nuclear envelope and for post-DSB synapsis, is also required for early pre-DSB homolog pai

216 ndependently of meiotic chromosome synapsis, synapsis itself generates additional compaction that mat

217 We propose that once pairing or synapsis juxtaposes homologues, exclusion of Mek1 is nec

218 equence important for chromosome pairing and synapsis may be the culprit.

219 in which isolated RAG-RSS complexes undergo synapsis mediated by RAG protein-protein interactions.

220 ins that is also highlighted in a screen for synapsis mutants.

221 are absent--Ph1 affects neither the level of synapsis nor the number of MLH1.

222 rtial pairing, recombination initiation, and synapsis occur in the absence of wild-type Rad50 catalyt

223 entre-located DQ52-to-J(H) joining, in which synapsis occurs by diffusion(2).

224 When Fpr3 and Zip3 are absent, synapsis occurs even in a mutant that fails to initiate

225 Synapsis occurs when a particular recombination pathway

226 We found that synapsis occurs with a high affinity (Kd = 10 nM) and is

227 that pol mu alone can mediate efficient NHEJ synapsis of 3' overhangs that have at least 1 nt microho

228 Molecular mechanisms underlying synapsis of activation-induced deaminase (AID)-targeted

229 for this directionality is due to selective synapsis of attP and attB sites.

230 cessary and sufficient to achieve a flexible synapsis of blunt DNA ends, whereas either alone is not.

231 in the atm single mutant, prevents complete synapsis of chromosomes, and results in extensive and pe

232 Plectonemic intertwining favors only synapsis of closely linked transposon ends in the invert

233 critical step in V(D)J recombination is the synapsis of complementary (12/23) recombination signal s

234 53BP1 promotes CSR in part by mediating synapsis of distal DNA ends, and in addition, inhibits 5

235 hanges in locus conformation may control the synapsis of distant recombination signal sequences, and

236 ion and therefore suppresses the promiscuous synapsis of distant transposon ends, which initiate McCl

237 a vertebrate cell-free extract to show that synapsis of DNA ends occurs in at least two stages that

238 sonance energy transfer (FRET) to detect the synapsis of fluorescently labeled RSS oligonucleotides.

239 This is exemplified by improper pairing and synapsis of homologous chromosomes and altered processin

240 strand break repair which ensures the proper synapsis of homologous chromosomes and normal meiotic pr

241 Pairing and synapsis of homologous chromosomes during meiosis is cru

242 of meiotic prophase, incomplete and aberrant synapsis of homologous chromosomes, persistence of stran

243 se integrase-bound attP and attB and inhibit synapsis of integrase-bound attL and attR.

244 tive Cre K201A mutant is fully competent for synapsis of loxP sites, yet the inactive Y324F and R173K

245 have studied the energetics of Cre-mediated synapsis of loxP sites.

246 A key stage in meiosis is the synapsis of maternal and paternal homologous chromosomes

247 se plants reveal reduced homologous pairing, synapsis of nonhomologous chromosomes, reduced bivalents

248 on attL and attR recombination partners, and synapsis of partner complexes follows rapidly after thei

249 recombinase N-terminal domain, required for synapsis of recombination substrates and catalysis.

250 cells, chromosomes still hypercondense, but synapsis of sister chromatids is abolished.

251 core of a multiprotein complex that promotes synapsis of the broken DNA ends.

252 this suggests that processing occurred after synapsis of the ends.

253 ene stage of meiotic prophase with defective synapsis of the homologous chromosomes.

254 g of homologous chromosomes and the intimate synapsis of the paired homologs by the synaptonemal comp

255 chestrates a regulatory mechanism to enforce synapsis of the transposon ends before cleavage by the t

256 We also show that synapsis of the transposon ends is a prerequisite for th

257 ves to downregulate transposition by slowing synapsis of the transposon ends.

258 osis polymerase domain of LigD mediating the synapsis of two noncomplementary DNA ends revealed a var

259 a substrates and to some extent through poor synapsis of Vbeta and Jbeta substrates.

260 re of meiosis, mediating the stable pairing (synapsis) of homologous chromosomes during prophase I.

261 ecombination reaction is the association, or synapsis, of Cre-bound loxP sites to form a tetrameric p

262 When either synapsis or crossover formation is impaired, CHK-2 activ

263 75 phosphorylation does not alter chromosome synapsis or DSB repair, indicating that Mec1 separates c

264 s including incomplete homologous chromosome synapsis or persistent histone H2AX phosphorylation in f

265 omal replication, sister chromatid cohesion, synapsis or recombination.

266 The most critical step of NHEJ is synapsis, or the juxtaposition of the two DNA ends of a

267 s to conclude that RAG binding, bending, and synapsis precede catalysis.

268 modification of homologous recombination and synapsis, probably via adjustments of core structural co

269 escued the fertility of oocytes containing a synapsis-proficient, DSB repair-defective mutation in a

270 As synapsis progresses and linear stretches of Zip1 are for

271 microscopy (3D-SIM), we observed that normal synapsis progression was also disrupted in des10, a phen

272 protected from Mph1-mediated dissociation by synapsis protein Zip1.

273 In addition to the key NHEJ synapsis proteins, Ku, X4L4, and XLF, it has been sugges

274 us to each other and play redundant roles in synapsis, providing an explanation for why these genes h

275 leavage and effects on resolvase binding and synapsis, providing insight into the serine recombinase

276 ms in which recombination initiates prior to synapsis, recombination preferentially occurs in short 1

277 ultiprotein complexes that are essential for synapsis, recombination, and segregation of homologous c

278 osomal escape from MSCI without accompanying synapsis/recombination defects.

279 We find that XYY males, like synapsis/recombination mutants, display pachytene arrest

280 vivo assays, we demonstrate that productive synapsis requires a specific "R" interface involving res

281 Our computer simulation of the synapsis showed that the bend angles, phi, created in is

282 n meiosis and into the mechanisms regulating synapsis so that it occurs selectively between homologs.

283 ps of NHEJ is now also apparent for the NHEJ synapsis step.

284 s emerge independently of meiotic chromosome synapsis, synapsis itself generates additional compactio

285 es Tdp1, and a second step that occurs after synapsis that requires Exo1.

286 nisms eliminate meiotic cells with defective synapsis, thereby minimizing transmission of aneuploidy.

287 keleton mediate homolog pairing and restrict synapsis to homologous pairs of chromosomes.

288 ssing-over, functioning to couple chromosome synapsis to the formation of crossover-specific recombin

289 Chromosome synapsis triggers collapse of the elongated PAR structur

290 hen combined with a quantitative analysis of synapsis using loxP mutants, the structures explain how

291 unction for Pol beta in recombination and/or synapsis, we used conditional gene targeting to delete t

292 condensation is a key factor in stimulating synapsis, whereas decondensation may facilitate the inva

293 ruit DSB-associated protein foci and undergo synapsis, which is followed by chromosome fragmentation.

294 howed a significant defect in sex chromosome synapsis, which likely contributed to the germ cell loss

295 al relationships between DNA target sites at synapsis, which we investigate using nicked-circular DNA

296 some IV sequences are capable of pairing and synapsis, while the contiguous X portion of mnT12 lacks

297 translocation and find that mcr can undergo synapsis with a standard recombination signal sequence w

298 conformation on binding to attB that permits synapsis with attP.

299 sets displayed a mix of synaptic failure and synapsis with both homologous and nonhomologous partners

300 recent insights into the mechanisms of NHEJ synapsis with updates on other steps of NHEJ, such as DN