ライフサイエンスコーパス: synaptobrevin

1 imulations with an external force applied to synaptobrevin.

2 hment protein-25 (SNAP-25), syntaxin 1A, and synaptobrevin.

3 to the interaction between UNC-17/VAChT and synaptobrevin.

4 mal-associated protein of 23 or 25 kDa), and synaptobrevin.

5 to identify positive regulators of vesicular synaptobrevin.

6 ns such as epsin, protein kinase C-beta, and synaptobrevin.

7 rodimers, leading to an acceptor complex for synaptobrevin.

8 as been artificially depleted by deletion of synaptobrevin.

9 te and colocalizes with the synaptic marker, synaptobrevin.

10 s transmembrane helix proteins: syntaxin and synaptobrevin.

11 rotein encoding genes bruchpilot, liprin and synaptobrevin.

12 1 binds specifically and non-specifically to synaptobrevin.

13 SNAP-25 and Syntaxin1, the SNARE partners of synaptobrevin.

14 r regions of the vesicle-associated protein, synaptobrevin.

15 NMJs in mutant mouse embryos lacking either synaptobrevin 1 (Syb1(lew/lew) ) or synaptobrevin 2 (Syb

16 nesis) and synaptophysin, PSD95, synapsin 1, synaptobrevin 1, neurogranin, GAP43 and synaptopodin (sy

17 d synaptic-synaptophysin, PSD95, synapsin 1, synaptobrevin 1, neurogranin, GAP43 and synaptopodin in

18 , C2F did not bind the vesicle SNARE protein synaptobrevin-1 (VAMP-1).

19 The major neuronal vSNAREs, synaptobrevin-1 and synaptobrevin-2, are expressed at th

20 gs show that, in the absence neuronal vSNARE synaptobrevin-1 and synaptobrevin-2, evoked neurotransmi

21 e Ca(2+)-independent upon deletion of vSNARE synaptobrevin-1 and synaptobrevin-2.

22 oprecipitated syntaxin-1 and SNAP-25 but not synaptobrevin-1.

23 actor attachment protein receptor) proteins: synaptobrevin 2 (or vesicle-associated membrane protein

24 Interactions between synaptobrevin 2 (Sb2) and syntaxin 1A (Sx1A) can be read

25 e-associated protein of 25 kDa (SNAP25), and synaptobrevin 2 (Sb2).

26 g either synaptobrevin 1 (Syb1(lew/lew) ) or synaptobrevin 2 (Syb2 (-/-)), and those lacking both (Sy

27 on of the SNARE complex by the vesicle SNARE synaptobrevin 2 (syb2) and the two plasma membrane SNARE

28 The neuronal vesicular SNARE protein synaptobrevin 2 (syb2) is anchored in the vesicle membra

29 protein attachment protein receptor (SNARE) Synaptobrevin 2 (Syb2) is known for mediating neurotrans

30 domain (TMD) of the vesicle membrane protein synaptobrevin 2 (syb2).

31 s between the synaptic vesicle SNARE protein synaptobrevin 2 and the plasma membrane syntaxin1A/SNAP2

32 unilamellar vesicles doped with the v-SNARE synaptobrevin 2 by means of spinning-disc confocal micro

33 For example, the binary syntaxin-synaptobrevin 2 complex, in addition to the ternary comp

34 nts in astrocytes expressing the fluorescent synaptobrevin 2 derivative, synapto-pHluorin.

35 fected with green fluorescent protein-tagged synaptobrevin 2, a marker of synaptic vesicles.

36 ensitive factor attachment protein receptor) synaptobrevin 2, and contain both l-glutamate and d-seri

37 complexes that include the vesicular SNARE, synaptobrevin 2, and that the participation of 5RK in CD

38 tion on its substratum, the synaptic protein synaptobrevin 2, attached to the beads.

39 t the ionic layer by cuffing syntaxin 1A and synaptobrevin 2, similar to the action of SNAP25B; thus

40 In contrast, the apparent number of VAMP2/synaptobrevin 2, synaptophysin, and synaptogyrin demonst

41 rotein receptor (SNARE) complex, composed of synaptobrevin 2, syntaxin and synaptosome-associated pro

42 achment protein receptors complex, including synaptobrevin 2, syntaxin, and synaptosome-associated pr

43 ent protein (SNAP) receptor (SNARE) proteins synaptobrevin 2, syntaxin-1A, and SNAP-25 is the key ste

44 etween two synaptic proteins syntaxin 1A and synaptobrevin 2, using an atomic force microscope and th

45 of 25 kDa (SNAP25), syntaxin-1a (syx-1), and synaptobrevin 2, which is essential for many physiologic

46 unilamellar vesicles containing the v-SNARE synaptobrevin 2, which were docked and fused with lipid-

47 in FcepsilonRI-regulated exocytosis, whereas synaptobrevin 2- or VAMP-3-deficient mast cells did not.

48 tics with the results obtained for synthetic synaptobrevin 2-doped vesicles fusing with PSMs of the s

49 ins of syntaxin1A, SNAP25 (two peptides) and synaptobrevin 2.

50 2 (Delta324-339), block its interaction with synaptobrevin-2 (L348R), or extend the helix to promote

51 Addition of the V(C) peptide synaptobrevin-2 (syb(57-92)) increases the docking effic

52 spectroscopy methods, we find that vesicular synaptobrevin-2 (syb-2) in its monomeric prefusion confo

53 erated four variants of the synaptic v-SNARE synaptobrevin-2 (syb2) anchored to the membrane by lipid

54 tosomal-associated protein 25 (SNAP-25), and synaptobrevin-2 (Syb2).

55 complex composed of SNAP-25, syntaxin-1, and synaptobrevin-2 (sybII) proteins.

56 b(G76V), GFP, and a synaptic vesicle protein synaptobrevin-2 (Ub(G76V)-GFP-Syb2); (2) GFP-Syb2; or (3

57 cholesterol on fusion pore formation between synaptobrevin-2 (VAMP-2)-containing proteoliposomes and

58 naptic vesicle mimics containing full-length synaptobrevin-2 and full-length synaptotagmin-1 to plasm

59 hen bound to Munc18-1, preventing binding to synaptobrevin-2 and SNAP-25 to form the ternary SNARE co

60 Using recombinant fragments of synaptobrevin-2 and synaptotagmin C2 domains we were abl

61 sealing response was blocked by fragments of synaptobrevin-2 and the C2B domain of synaptotagmin VII.

62 nt on interactions between the vesicle SNARE synaptobrevin-2 and the plasma membrane SNAREs syntaxin-

63 hese residues are hydrophilic and constitute synaptobrevin-2 C-terminal flexible region.

64 ly due to fewer vesicles of reduced size and synaptobrevin-2 content.

65 The residue Y113 of synaptobrevin-2 flexible region was mutated to lysine an

66 omplexin interaction reduces the affinity of synaptobrevin-2 for the 1:1 complex, thereby retarding S

67 SPalpha), synaptophysin, synaptotagmin-1, or synaptobrevin-2 in their axons.

68 al neurons, but not in neurons obtained from synaptobrevin-2 knockout mice.

69 lipid-anchored syntaxin-1 and lipid-anchored synaptobrevin-2 lacking TMRs efficiently promoted sponta

70 y fluorescent tagging of the vesicle protein synaptobrevin-2 or by staining with the styryl dye FM4-6

71 n docking, but little effect on the rates of synaptobrevin-2 proteoliposome fusion.

72 Examining single particle fusion between synaptobrevin-2 proteoliposomes and planar-supported bil

73 These observations provide evidence that the synaptobrevin-2 TMD catalyzes the fusion process by its

74 , we show that conformational flexibility of synaptobrevin-2 TMD is essential for efficient Ca(2+)-tr

75 Synaptobrevin-2 transmembrane domain is embedded into th

76 he absence of synaptic vesicle SNARE protein synaptobrevin-2 whereas the increase in spontaneous fusi

77 BPB also quenched fluorescence of VAMP (synaptobrevin-2)-EGFP, thus indicating the timing of fir

78 e vesicular SNARE protein VAMP2 (also called synaptobrevin-2).

79 Synaptobrevin-2, a snap receptor (SNARE) protein, partic

80 major neuronal vSNAREs, synaptobrevin-1 and synaptobrevin-2, are expressed at the developing neuromu

81 aptic SNARE proteins SNAP-25, syntaxin-1 and synaptobrevin-2, as well as by an age-dependent reductio

82 absence neuronal vSNARE synaptobrevin-1 and synaptobrevin-2, evoked neurotransmission is completely

83 SNARE complex consists of the three proteins synaptobrevin-2, syntaxin, and synaptosomal-associated p

84 uxtamembranous mutation in the SNARE-protein synaptobrevin-2, which presumably impairs force transfer

85 contained synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPalpha immunoreactivity, respect

86 Among synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPalpha-IR varicosities, 98% +/-

87 Our present results show that synaptobrevin-2-like immunoreactivity (-LIR) is present

88 ste cells with synapses display SNAP-25- and synaptobrevin-2-like immunoreactivity (LIR).

89 Synaptobrevin-2-LIR also colocalizes with immunoreactivi

90 Synaptobrevin-2-LIR colocalizes with SNAP-25-, serotonin

91 old immunoelectron microscopy, we found that synaptobrevin-2-LIR is associated with synaptic vesicles

92 However, approximately 27% of the synaptobrevin-2-LIR taste cells do not display IP3R3-LIR

93 rom taste cells onto nerve processes express synaptobrevin-2-LIR, as well as some taste cells without

94 oth type II and type III taste cells display synaptobrevin-2-LIR.

95 All IP3R3-LIR taste cells express synaptobrevin-2-LIR.

96 upon deletion of vSNARE synaptobrevin-1 and synaptobrevin-2.

97 ranes that kinetically alters the binding of synaptobrevin-2.

98 lex result in an additional interaction with synaptobrevin-2/VAMP2 (vesicle-associated membrane prote

99 ractions of native alpha-synuclein with both synaptobrevin-2/VAMP2 and anionic lipids.

100 In contrast, the R-SNARE protein synaptobrevin-2/VAMP2 contributes to both regulated and

101 its C-terminal SNARE motif and competes with synaptobrevin-2/VAMP2 for the SNARE-complex assembly.

102 helix 12 in Munc18 within domain 3a leads to synaptobrevin-2/VAMP2 interaction and SNARE complex form

103 n complexes composed of syntaxin-1, SNAP-25, synaptobrevin-2/VAMP2, and Munc18-1.

104 amma-synuclein was not able to interact with synaptobrevin-2/VAMP2.

105 ynuclein directly bound to the SNARE-protein synaptobrevin-2/vesicle-associated membrane protein 2 (V

106 We monitored the distribution of synaptobrevin, a vesicle protein required for exocytosis

107 b(G76V)-GFP-Syb2 may compete with endogenous synaptobrevin, acting as a gain-of-function mutation tha

108 RE complex formed by syntaxin-1, SNAP-25 and synaptobrevin, allowing exquisite regulation of neurotra

109 vere decrease in exocytosis than deletion of synaptobrevin alone.

110 The synaptic vesicle protein synaptobrevin (also called VAMP, vesicle-associated memb

111 ing of individual RGC axons coexpressing GFP-synaptobrevin and DsRed in the intact Xenopus brain demo

112 r vesicle-associated membrane protein (VAMP)/synaptobrevin and found reduced neural-crest-derived cel

113 a conserved set of SNARE proteins: vesicular synaptobrevin and plasma membrane syntaxin and SNAP-25.

114 rization of structural elements in prefusion synaptobrevin and providing a framework for interpreting

115 s including increased levels of synapsin and synaptobrevin and reduced levels of NMDA receptor subuni

116 ocking/fusion machinery that binds with VAMP/synaptobrevin and SNAP-25 to form the SNARE complex.

117 s on the SNARE complex formed by syntaxin-1, synaptobrevin and SNAP-25, as well as on complexins, whi

118 ARE helix is available for interactions with Synaptobrevin and SNAP-25.

119 brium the Cpx AH forms tight links with both synaptobrevin and SNAP25.

120 ic complexes, after stimulation up to 25% of synaptobrevin and synaptophysin are present in homo- and

121 Whereas at rest less than 10% of the total synaptobrevin and synaptophysin could be chemically cros

122 evealed selective reduction of the levels of synaptobrevin and synaptophysin in synaptosomes from stg

123 ." MISTs based on the vesicle proteins VAMP2/Synaptobrevin and Synaptophysin induced rapid ( approxim

124 but represent distinct functional states of synaptobrevin and synaptophysin that are modulated in pa

125 the ASP was compromised if disc cells lacked Synaptobrevin and Synaptotagmin-1 (which function in ves

126 Donor vesicles contain reconstituted synaptobrevin and synaptotagmin-1.

127 The expression of synaptobrevin and syntaxin 3, TA proteins essential for

128 propensities of the transmembrane domains of synaptobrevin and syntaxin.

129 es form between the synaptic vesicle protein synaptobrevin and the plasma membrane proteins syntaxin

130 The synaptic vesicle SNARE synaptobrevin and the plasma membrane SNAREs syntaxin-1

131 omplexes composed of the vesicular (v)-SNARE synaptobrevin and the target (t)-SNAREs Snap-25 and synt

132 as been proposed that Munc18-1 also binds to synaptobrevin and to the SNARE four-helix bundle and tha

133 ow demonstrate that Munc18-1 indeed binds to synaptobrevin and to the SNARE four-helix bundle.

134 es required diaphanous, SCAR, Neuroglian and Synaptobrevin, and both the Hh gradient and Hh signaling

135 interactions among syntaxin, SNAP23/SNAP25, synaptobrevin, and complexin by employing a newly develo

136 The SNAREs Syntaxin-1, Synaptobrevin, and SNAP-25 play a central role in membra

137 Futsch, a marker of neurites, and Synapsin, Synaptobrevin, and Synaptogamin, proteins involved in ne

138 onal SNARE complexes consisting of syntaxin, synaptobrevin, and synaptosome-associated protein of 25

139 three SNARE proteins, syntaxin, SNAP-25, and synaptobrevin, and the SM protein, Munc18-1.

140 25), and vesicle-associated membrane protein/synaptobrevin are collectively called SNAP receptor (SNA

141 ilt and insertion depth of membrane-embedded synaptobrevin are determined.

142 s involving the SNARE motifs of syntaxin and synaptobrevin as well as those of syntaxin and synaptoso

143 ARE protein machinery syntaxin, SNAP-25, and synaptobrevin, as is used by synapses in the central ner

144 Four mutations in the VAMP/synaptobrevin-associated protein B (VAPB) gene have been

145 VAMP/synaptobrevin-associated proteins (VAPs) contain an N-te

146 w)Syb2 (-/-) NMJs, our findings suggest that synaptobrevin-based SNARE complexes play a critical role

147 Moreover, these findings suggest that synaptobrevin-based SNARE complexes play critical roles

148 NARE complex assembly, and autoinhibition of synaptobrevin binding contributes to enabling regulation

149 f of the SNARE motif from the neuronal SNARE synaptobrevin binds to membranes, which appeared to cont

150 or of vesicle docking with only syntaxin and synaptobrevin, but have been confirmed by other experime

151 involved in axonal trafficking of FasII and synaptobrevin, but not syntaxin.

152 Munc18-1 binds to synaptobrevin, but the relevance of this interaction and

153 the TM helix in the simulation detached the synaptobrevin C-terminus from the vesicle's inner-leafle

154 h the Cpx AH clamps fusion by binding to the synaptobrevin C-terminus, thus preventing full SNARE zip

155 heless, by a single amino acid substitution, synaptobrevin can be driven to dimerize with the same af

156 NMDA receptor blockade: BDNF maintained GFP-synaptobrevin cluster density by maintaining their addit

157 gonists APV or MK801 transiently induced GFP-synaptobrevin cluster dismantling, but did not significa

158 ocking antibodies significantly enhanced GFP-synaptobrevin cluster elimination, a response that was p

159 ds along the groove between the syntaxin and synaptobrevin coils.

160 cellubrevin rescues synaptic transmission in synaptobrevin-deficient neurons but that deletion of bot

161 ue insertion restored spontaneous release in synaptobrevin-deficient neurons.

162 yt) strongly stimulated membrane fusion when synaptobrevin densities were similar to those found in n

163 the SNARE motif did not significantly impair synaptobrevin-dependent exocytosis, whereas insertion of

164 tants, we found that the majority of surface synaptobrevin derives from fusion of synaptic vesicles a

165 nd that the individual proteins syntaxin and synaptobrevin disrupt membranes so as to favor formation

166 ns but that deletion of both cellubrevin and synaptobrevin does not cause a more severe decrease in e

167 The synaptic vesicle protein synaptobrevin engages with syntaxin and SNAP-25 to form

168 synaptic vesicles and that, in steady state, synaptobrevin equilibrates throughout the axon.

169 a synaptic vesicle-associated protein termed synaptobrevin, exhibit similar convulsion phenotypes fol

170 ompetes with the SNARE four-helix bundle and synaptobrevin for Munc18-1 binding.

171 monstrate that FTY720 can activate vesicular synaptobrevin for SNARE complex formation and enhance ex

172 The SNARE proteins, syntaxin, SNAP-25 and synaptobrevin form a tertiary complex essential for vesi

173 In the SNARE complex, the SNARE motif of synaptobrevin forms a 55-residue helix, but it has been

174 Recombinant synaptobrevin forms a small amount of dimer and higher o

175 sing membranes, it does not act by releasing synaptobrevin from synaptic vesicle restriction.

176 legans motor neurons by using a pH-sensitive synaptobrevin GFP fusion protein, synaptopHluorin.

177 Direct visualization of Synaptobrevin-GFP transport in living animals demonstrat

178 synaptic vesicle markers (Synaptotagmin and Synaptobrevin-GFP) and clear-core vesicles along Drosoph

179 First, we demonstrate that a synaptobrevin-GRASP chimera functions as a powerful acti

180 esults show that the transmembrane domain of synaptobrevin has only a modest ability to self-associat

181 A syntaxin/synaptobrevin heterodimer is able to form under oxidizin

182 To gauge the magnitude of synaptobrevin homodimerization, we used the well-charact

183 Live imaging of SYNA, a synaptobrevin homologue, and SECC, an exocyst component,

184 physin results in the defective retrieval of synaptobrevin II (sybII) from the plasma membrane during

185 membrane domain (JMD) of the vesicular SNARE Synaptobrevin II (SybII) profoundly impairs priming of g

186 er release is mediated by the SNARE proteins synaptobrevin II (sybII, also known as VAMP2), syntaxin,

187 exocytosis, we expressed the SNARE motif of synaptobrevin II to prevent the formation of SNARE compl

188 Green fluorescent protein (GFP)-tagged synaptobrevin II was used to visualize presynaptic speci

189 complexes formed between the SNARE motifs of synaptobrevin II, SNAP-25, and syntaxin play an essentia

190 The three neuronal SNAREs syntaxin-1A, synaptobrevin-II (VAMP), and SNAP-25A were expressed sep

191 imulations of the prefusion configuration of synaptobrevin in a lipid bilayer, aimed at characterizin

192 evidence showing the dual role of SNAP25 and synaptobrevin in both exocytosis and slow endocytosis at

193 NMR data for full-length synaptobrevin in dodecylphosphocholine micelles reveals

194 However, the membrane-embedded structure of synaptobrevin in its prefusion state, which determines i

195 o contradict previous biophysical studies of synaptobrevin in liposomes.

196 Syntaxin and synaptobrevin in opposing membranes were both necessary

197 protein surface concentrations reported for synaptobrevin in synaptic vesicles and with an optimally

198 easable backbone of sphingolipids, activates synaptobrevin in synaptic vesicles to form the SNARE com

199 , as well as evidence for gene expression of synaptobrevin in the lamellar cells led to the hypothesi

200 ng vesicles could mix, suggesting a role for synaptobrevin in the separation of the two pools.

201 paired recordings, we find that cleavage of synaptobrevin in unprimed vesicles leads to an eventual

202 Consistent with the role of synaptobrevin in vesicle fusion, sphingosine upregulated

203 synapses lacking the vesicular SNARE protein synaptobrevin in which synaptic responses are severely d

204 ast, cleavage of the synaptic vesicle SNARE (synaptobrevin) in conjunction with deletion of the vesic

205 other conformational states of the syntaxin*synaptobrevin interaction in addition to those observed

206 mes reconstituted with full-length v-SNAREs (synaptobrevin) into planar lipid bilayers containing bin

207 Synaptobrevin is a vesicle-associated membrane protein (

208 most of the SNARE motif of membrane-anchored synaptobrevin is accessible for SNARE complex formation.

209 d heteromultimerization of synaptophysin and synaptobrevin is increased up to 6-fold.

210 ues, we found that not only SNAP25, but also synaptobrevin is involved in slow endocytosis.

211 Importantly, the Munc18-1 binding site on synaptobrevin is located at the C-terminus of its SNARE

212 osslinking studies show that dimerization of synaptobrevin is promoted by oxidizing agents.

213 complex with the core proteins, SNAP-25 and synaptobrevin, is also required for the association with

214 educed the number of red fluorescent protein-synaptobrevin-labeled presynaptic specializations per ax

215 ition enables syntaxin and SNAP-25 to engage synaptobrevin, leading to membrane fusion.

216 nredundant functions in the recycling of the synaptobrevin-like v-SNARE Snc1 from early endosomes.

217 sults in only mild and occasional defects in synaptobrevin localization.

218 Our data suggest that synaptobrevin mediates Ca2+-triggered exocytosis by tigh

219 taxin 1A and SNAP-25 and the vesicle v-SNARE synaptobrevin, mediates the fusion of 2 membranes.

220 The stimulus dependence of synaptophysin and synaptobrevin multimers indicates that the complexes are

221 eins involved in the same molecular process (synaptobrevins, Munc13-1/2) do not cause degeneration.

222 ene family: synaptobrevin (syb) and neuronal synaptobrevin (n-syb).

223 nd SNAP-25 on the target plasma membrane and synaptobrevin on the vesicular membrane.

224 Synaptobrevins or VAMPs are vesicle-associated membrane

225 disrupts the interaction of the Cpx AH with synaptobrevin, partially imitating the cpx null phenotyp

226 nsights suggest that sphingosine acts on the synaptobrevin/phospholipid interface, defining a novel f

227 on-specific synaptic v-SNARE n-syb (neuronal Synaptobrevin) plays a key role during synaptic vesicle

228 ed rats had higher density of synaptophysin-/synaptobrevin-positive puncta in DG and CA1 subregions o

229 NMR analyses of synaptobrevin reconstituted into nanodiscs and into lipo

230 the transmembrane sequences of syntaxin and synaptobrevin reveal structural models that correlate wi

231 d membrane protein type v-SNARE proteins (or synaptobrevins) reveals characteristic alterations to ve

232 o suspend the bead off the cantilever we use synaptobrevin's molecular interaction with another synap

233 m hemifusion to complete fusion, the role of synaptobrevin's TM domain association in the fusion proc

234 ic SNARE protein where a portion of neuronal synaptobrevin (Sb) is fused to Snc2p, a Sb ortholog requ

235 ol (PEG) to investigate the influence of the synaptobrevin (SB) TMD with an attached JMR (SB-JMR-TMD)

236 cytosis machinery, including SNARE proteins (synaptobrevin, SNAP25, and syntaxin), is needed to coini

237 SNARE complex lacking the C-terminus of the synaptobrevin SNARE motif (SNAREDelta60) suggested that

238 by NMR experiments showing that the soluble synaptobrevin SNARE motif does not bind to liposomes, ev

239 ne anchoring, our data show that most of the synaptobrevin SNARE motif has a remarkable reluctance to

240 eptide that spans the C-terminal half of the synaptobrevin SNARE motif.

241 t of Munc18: it promotes the proper syntaxin/synaptobrevin subconfiguration during assembly of the te

242 o characterized members of this gene family: synaptobrevin (syb) and neuronal synaptobrevin (n-syb).

243 , fragments of the toxin substrate proteins, synaptobrevin (Syb) or synaptosome-associated protein of

244 s indicated that the vesicular SNARE protein synaptobrevin (syb) was dispensable for docking.

245 naptogenesis, namely Synaptophysin (Syn) and Synaptobrevin (Syb).

246 Synaptobrevin (Syb)/vesicle-associated membrane protein

247 sterol-rich compartments (STRIC) labeled by synaptobrevin Syb1.

248 Two homologous isoforms of synaptobrevin, Syb1/VAMP1 and Syb2/VAMP2, exhibit distin

249 Synaptobrevin (sybII) is another abundant integral SV pr

250 process-the vesicle-associated proteins VAMP/synaptobrevin, synaptotagmin, and Rab3-are each immobile

251 We propose that synaptobrevin, synaptotagmin, and UNC-104 are specific c

252 ptic vesicle-related proteins, such as VAMP (synaptobrevin), syntaxin-1, synaptophysin, synapsin Ia/b

253 The neuronal SNARE complex formed by synaptobrevin, syntaxin and SNAP-25 plays a central role

254 e formation of the SNARE complexes involving synaptobrevin, syntaxin and SNAP-25 that play an essenti

255 cysteine residue in the same location as in synaptobrevin, syntaxin dimerization is not promoted by

256 hment protein receptor) complex, composed of synaptobrevin, syntaxin, and SNAP25, forms the essential

257 ontaining liposomes and liposomes containing synaptobrevin (T and V liposomes, respectively), and flu

258 that there is a plasma membrane reservoir of synaptobrevin that is supplied by the synaptic vesicle c

259 0 nm) liposomes reconstituted with different synaptobrevin (the SNARE present in synaptic vesicles) d

260 ural properties of both wild-type and mutant synaptobrevin, the effects of C-terminal additions on ti

261 We study the oligomerization of synaptobrevin TMD using ensembles of molecular dynamics

262 hen examined the structural requirements for synaptobrevin to function in exocytosis.

263 ransmembrane region abolished the ability of synaptobrevin to mediate Ca2+-evoked exocytosis.

264 ynaptopHluorin created by the fusion of VAMP/synaptobrevin to the pH-sensitive super-ecliptic green f

265 Additionally, the synaptobrevin transmembrane domain can promote lipid mix

266 The synaptobrevin transmembrane domain has an alpha-helical

267 ports have been presented on the role of the synaptobrevin transmembrane domain in mediating importan

268 semble the homodimer interface formed by the synaptobrevin transmembrane domain.

269 structure of a superclamp mutant bound to a synaptobrevin-truncated SNARE complex.

270 lexin-I accessory helix does not insert into synaptobrevin-truncated SNARE complexes in solution, and

271 directly fused to the presynaptic protein n-synaptobrevin, under the control of the pan-neuronal pro

272 in the absence of the vesicle SNARE protein synaptobrevin (VAMP), activity-dependent and spontaneous

273 or (SNARE) proteins syntaxin-1, SNAP-25, and synaptobrevin/VAMP (vesicle-associated membrane protein)

274 rotoxin requires the synaptic SNARE-proteins synaptobrevin/VAMP and SNAP-25, and, at least partly, th

275 Synaptophysin and synaptobrevin/VAMP are abundant synaptic vesicle protein

276 Vesicle Associated Membrane Protein (VAMPs: synaptobrevin/VAMP-2, cellubrevin/VAMP-3, TI-VAMP/VAMP-7

277 mice did express apparently higher levels of synaptobrevin/VAMP-2.

278 n-1, SNAP-25) or Munc18-1, but not v-SNAREs (synaptobrevins/VAMP1/2/3 using tetanus neurotoxin (TeNT)

279 e membrane insertion sequence of the v-SNARE synaptobrevin/vesicle-associated membrane protein (VAMP)

280 SNAP-25, syntaxin 1A, and synaptobrevin/vesicle-associated membrane protein (VAMP)

281 Synaptobrevin/vesicle-associated membrane protein 2 (VAM

282 Deletion of synaptobrevin/vesicle-associated membrane protein, the m

283 Docking and fusion of reconstituted synaptobrevin vesicles to target SNARE complex-containin

284 rane had no significant effect on docking of synaptobrevin vesicles.

285 The surface synaptobrevin was enriched near active zones, and its sp

286 ease in multimerization of synaptophysin and synaptobrevin was only observed in intact but not in lys

287 We estimated that 30% of synaptobrevin was present in the plasma membrane.

288 The relative amount of fluorescent synaptobrevin was substantially lower at synapses of kno

289 s with only one synaptobrevin, whereas 23-30 synaptobrevins were necessary for efficient lipid mixing

290 s maximal with small liposomes with only one synaptobrevin, whereas 23-30 synaptobrevins were necessa

291 Hypomorphic mutations in syntaxin-1A or n-synaptobrevin, which also disrupt neurotransmitter relea

292 in receptors (SNAREs) SNAP-25, syntaxin, and synaptobrevin, which constitute part of the synaptic ves

293 s the SNARE proteins syntaxin-1, SNAP-25 and synaptobrevin, which form a tight "SNARE complex", and M

294 ronal SNARE proteins, SNAP-25, syntaxin, and synaptobrevin, which were reconstituted into vesicles.

295 t, highly tilted state for membrane-embedded synaptobrevin with a helical connection between the tran

296 ane domain, is markedly improved by a mutant synaptobrevin with an isoleucine-to-aspartate substituti

297 Cleavage of the other SNAREs (synaptobrevin with Botx/D or SNAP-25 with Botx/A) failed

298 vesicles bearing the vesicle fusion protein synaptobrevin with supported planar membranes harboring

299 Surprisingly, however, synaptobrevin with the 12-residue but not the 24-residue

300 osomes containing the synaptic vesicle SNARE synaptobrevin (with or without the Ca(2+) sensor synapto