ライフサイエンスコーパス: synthetic gene

1 abeled proteotypic peptides generated from a synthetic gene.

2 -His(6)-tag protein, using a codon-optimized synthetic gene.

3 ant vaccinia virus constructs expressing the synthetic gene.

4 nant human PC-TP in Escherichia coli using a synthetic gene.

5 5)N-labeled cytochrome b(5) expressed from a synthetic gene.

6 ein to high yield in Escherichia coli from a synthetic gene.

7 arkedly less stable than that encoded by the synthetic gene.

8 ific DNA endonuclease, to remove errors from synthetic genes.

9 , LPS O-antigen synthetic genes, and capsule synthetic genes.

10 schema for standardized data description of synthetic genes.

11 s using a previously undescribed method with synthetic genes.

12 s aquaticus) to remove failure products from synthetic genes.

13 dy with increased expression of the capsular synthetic genes.

14 turally occurring and nonnaturally occurring synthetic genes.

15 using a transcriptional signaling cascade of synthetic genes.

16 ntly from CPA inactivation for endogenous or synthetic genes.

17 NA molecules produced by rationally designed synthetic genes.

18 h repressive histone modifiers to inhibit BA synthetic genes.

19 the expression and regulation of the starch synthetic genes.

20 and was co-expressed with most of the starch synthetic genes.

21 naturally occurring proteins were encoded in synthetic genes; 56 were found to be expressed and solub

22 We designed the synthetic gene '5GHPV3' by selecting conserved regions f

23 ar use in the analysis of these genes is the synthetic gene, a nucleotide sequence designed to the sp

24 re in Escherichia coli by using an optimized synthetic gene and cold-adapted chaperonins.

25 OX was expressed in Escherichia coli using a synthetic gene and found to be a stable, monomeric, FAD-

26 iption terminator that is placed between the synthetic gene and reporter gene.

27 y 30-50% reduction in the expression of ATRA synthetic genes and a 120% increase in the expression of

28 fects on protein expression, we designed 285 synthetic genes and determined corresponding expression

29 Up-regulation of PGC-1beta and lipid synthetic genes and down-regulation of silence regulator

30 The study consists of two parts: tests with synthetic genes and experiments starting with whole LM c

31 We expressed and characterized the synthetic genes and found no significant effect of the D

32 nsively is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling t

33 Expression analyses of cell-wall synthetic genes and wall-related transcription factors i

34 la pathogenicity island genes, LPS O-antigen synthetic genes, and capsule synthetic genes.

35 sequence data, design and build libraries of synthetic genes, and test them for activity in vivo usin

36 n Escherichia coli (200 nM culture), using a synthetic gene approach.

37 For each of six targets, just two to six synthetic genes are made for expression in Escherichia c

38 Here we show that eight synthetic genes are sufficient to promote filament assem

39 To bridge this gap a synthetic gene, ARG8m, designed to specify an arginine b

40 Next a synthetic gene array identifies nonessential genes that

41 We propose that the cobalamin synthetic genes, as well as genes providing cobalamin-de

42 e is a global down-regulation of cholesterol synthetic genes, as well as SREBP-2, in the brains of di

43 larm pheromone for many pest aphids, using a synthetic gene based on a sequence from peppermint with

44 We used a synthetic gene based on mammalian GTP cyclohydrolase I,

45 A synthetic gene based on the primary sequence of the matu

46 We designed synthetic genes based on P. falciparum, Escherichia coli

47 n of not only previously identified glycogen synthetic genes, but also a significant regulon of genes

48 iterations, we were able to reduce errors in synthetic genes by >16-fold, yielding a final error rate

49 Designing synthetic genes by hand is a time-consuming and error-pr

50 The variant GFP encoded by the synthetic gene can be expressed at a high level, forming

51 The synthetic gene can be optimized for expression and const

52 essed and purified in Escherichia coli using synthetic genes, characterized regarding biochemical pro

53 dations, we design the CASwitch, a mammalian synthetic gene circuit based on combining two well-known

54 atively characterized an inducible, bistable synthetic gene circuit controlling the expression of a b

55 imple form, making them especially useful as synthetic gene circuit design equations.

56 mputing, neuro-inspired models can transform synthetic gene circuit design in a manner that is reliab

57 A limiting factor in synthetic gene circuit design is the number of independe

58 the antithetic integral feedback motif in a synthetic gene circuit in mammalian cells.

59 titatively by single-cell data analysis of a synthetic gene circuit integrated in human kidney cells.

60 be engineered predictably using exchangeable synthetic gene circuit modules to sense and integrate mu

61 SPR-Cas9 genome editing of iPSCs to create a synthetic gene circuit that senses changing levels of en

62 e tested different topologies and verified a synthetic gene circuit with mutual inhibition and auto-a

63 eedback control mediated by degronLOCKR on a synthetic gene circuit(9), to quantify the feedback capa

64 order to control transcription from a simple synthetic gene circuit.

65 Adaptability of synthetic gene circuits across different organisms could

66 Synthetic gene circuits allow the behavior of living cel

67 equire accurate predictive design of complex synthetic gene circuits and accompanying large sets of q

68 We review how CRISPR can be used to build synthetic gene circuits and discuss recent advances in C

69 The mutual interactions between the synthetic gene circuits and the host growth could cause

70 Synthetic gene circuits are designed to program new biol

71 Synthetic gene circuits are emerging as a versatile mean

72 However, synthetic gene circuits are often unreliable, as changes

73 However, as synthetic gene circuits become larger and more complicat

74 ts an essential parameter in the dynamics of synthetic gene circuits but typically is not explicitly

75 This work demonstrates that synthetic gene circuits can be engineered to be robust t

76 Cells endowed with synthetic gene circuits can control the localization, ti

77 he creation of engineered cells that harbour synthetic gene circuits capable of biological sensing an

78 ise a robust platform for building mammalian synthetic gene circuits capable of precisely modulating

79 Here, using synthetic gene circuits constructed in yeast, we find th

80 illustration, we apply this strategy to two synthetic gene circuits exhibiting anomalous behaviors.

81 gration, and analysis of several large scale synthetic gene circuits for artificial tissue homeostasi

82 In this work, synthetic gene circuits for organofluorine biosynthesis

83 d our work has implications in the design of synthetic gene circuits for this purpose.

84 competition on the deterministic behavior of synthetic gene circuits has been studied, its effects on

85 Recently, synthetic gene circuits have become promising tools to a

86 the development and clinical translation of synthetic gene circuits in diverse human cell types and

87 d TF signaling using mathematical models and synthetic gene circuits in Escherichia coli.

88 integrate protein-level tuning, noise-aware synthetic gene circuits into a well-defined human genomi

89 ests host species, therapeutic payloads, and synthetic gene circuits of engineered bacteria within mu

90 is framework, we demonstrate construction of synthetic gene circuits of up to 64 kb in size comprisin

91 The construction of synthetic gene circuits relies on our ability to enginee

92 Engineering of cell fate through synthetic gene circuits requires methods to precisely im

93 The construction of synthetic gene circuits requires the rational combinatio

94 These synthetic gene circuits reveal a unique approach to supp

95 Synthetic gene circuits that combine DNA, protein, and R

96 We developed inducible synthetic gene circuits that generate varying degrees of

97 such as designing and implementing intricate synthetic gene circuits that perform complex sensing and

98 Synthetic gene circuits that precisely control human cel

99 synthesis pathways, we constructed inducible synthetic gene circuits that regulate bacterial encapsul

100 We found these synthetic gene circuits to be stable and robust in the l

101 develop positive and negative feedback-based synthetic gene circuits to decouple noise from the mean

102 operties that can be harnessed by native and synthetic gene circuits to provide greater control over

103 erstanding natural promoters and engineering synthetic gene circuits with desired expression properti

104 mains a significant challenge for assembling synthetic gene circuits with tested modules as they ofte

105 le principle that adds a layer of control to synthetic gene circuits, allowing dynamic regulation of

106 l fate determination by viruses, dynamics of synthetic gene circuits, and constraints on evolutionary

107 facilitate viral vector production, control synthetic gene circuits, and other purposes.

108 orm for integrated logic and memory by using synthetic gene circuits, and we demonstrated the impleme

109 een made in the design and implementation of synthetic gene circuits, but real-world applications of

110 o systematically engineer the performance of synthetic gene circuits, expanding the current toolkit f

111 When these insulators are used to join synthetic gene circuits, the behavior of layered circuit

112 ynamic auxin response interplay trackable by synthetic gene circuits, thereby offering instructions f

113 By combining directed evolution and synthetic gene circuits, we developed a unique self-modu

114 es a unique library of components for use in synthetic gene circuits.

115 antitumor therapeutic molecules via several synthetic gene circuits.

116 c modeling, and experimentally, using simple synthetic gene circuits.

117 ural biological networks and for engineering synthetic gene circuits.

118 ontrol of endogenous signalling pathways and synthetic gene circuits.

119 allenge for maintaining reliable function in synthetic gene circuits.

120 unveil nonlinear resource competition within synthetic gene circuits.

121 sed economy through the predictive design of synthetic gene circuits.

122 l as a robust modelling method for designing synthetic gene circuits.

123 for modulating and expanding the function of synthetic gene circuits.

124 control of cell metabolism, cell biology and synthetic gene circuits.

125 their components can be used for large-scale synthetic gene circuits.

126 gy has developed numerous parts for building synthetic gene circuits.

127 recognition, costimulation, and addition of synthetic genes, circuits, knockouts and base edits to f

128 A synthetic gene coding for a single-chain fragment (ScFv)

129 We generated a synthetic gene coding for CP4 EPSP synthase and characte

130 A synthetic gene coding for gamma-gliadin 1 (GG1) was expr

131 A bacterial expression system using a synthetic gene coding for the 215-amino acid, full-lengt

132 A synthetic gene coding for the Chlamydia trachomatis sero

133 Synthetic genes coding for 2 hybrid proteins consisting

134 Plasmids that contain synthetic genes coding for small oligoribonucleotides ca

135 The transcription factors regulate synthetic genes coding for small regulatory RNAs (called

136 ll six HCV genotypes were selected to design synthetic genes coding for this antigenic region.

137 This synthetic gene complementation approach can also be used

138 h repressors were examined in the context of synthetic gene complexes containing modular promoters an

139 A synthetic gene consisting of the reverse transcriptase (

140 Using synthetic gene constructs for recombinant expression of

141 With these synthetic gene constructs it was possible to demonstrate

142 A synthetic gene containing an E. coli codon bias was clon

143 farnesoid X receptor, is recruited to the BA synthetic genes Cyp7a1 and Cyp8b1 and the BA uptake tran

144 hematical function, parameterized using this synthetic gene data set, which enables computation of pr

145 Here we present the Synthetic Gene Database (SGDB): a relational database th

146 A major challenge in synthetic gene delivery is to quantitatively predict the

147 Self-assembled synthetic gene delivery systems represent the bottom-up

148 Compared with viral vectors, synthetic gene-delivery systems, such as liposomes and p

149 many modules to provide a platform for rapid synthetic gene design for multikilobase sequences.

150 eriving rare earth elements from macroalgae, synthetic gene drives in plants, and low-emission cement

151 Synthetic gene drives promise more efficient approaches

152 Synthetic gene drives provide one set of tools, but thei

153 e utilized to create, streamline and improve synthetic gene drives, this is rapidly changing.

154 Typically, synthetic genes encode the same product as the gene of i

155 In this study, a synthetic gene encoding a C4V3 recombinant protein, know

156 ng these chimeric antigens, we constructed a synthetic gene encoding a hybrid protein that combined b

157 In this study, a plant optimized synthetic gene encoding for the LTB-ST fusion protein ha

158 us recombination in yeast we have inserted a synthetic gene encoding human ornithine transcarbamylase

159 tin gene in Schizosaccharomyces pombe with a synthetic gene encoding R-Sp-actin reduces Arp2/3-based

160 A synthetic gene encoding the fusion protein (Ala-Hyp)(51)

161 ian cells transfected with a codon-optimized synthetic gene encoding the KcsA protein expressed K+-se

162 A synthetic gene encoding the tandem affinity purification

163 We obtained synthetic genes encoding 129 of the network-'hallucinate

164 testing, involving in total the assembly of synthetic genes encoding 7,896 designs and assessment of

165 Synthetic genes encoding Nanoluciferase polypeptides wer

166 Early tests of the hypothesis using synthetic genes encoding only clustered noncontiguous Hy

167 cts arabinosylation, we designed a series of synthetic genes encoding repetitive (Ser-Pro(2))(n), (Se

168 ples that have proven difficult to clone and synthetic genes encoding toxic products.

169 Yet design and construction of synthetic genes, especially those coding for large prote

170 (2) The synthetic gene expression contains predictive signals as

171 Specifically, using both real and synthetic gene expression data sets, we compared the qua

172 in this paper we create tens of thousands of synthetic gene expression outputs for bacterial promoter

173 signs for genetic controllers which maintain synthetic gene expression over time.

174 ictive performance using a large database of synthetic gene expression profiles.

175 caffolds provide a powerful way to construct synthetic gene expression programs for a wide range of a

176 The synthetic gene expression system was validated with two

177 alcohol may modulate both apoptotic and fat synthetic gene expression through homocysteine-induced E

178 : (1) Our pipeline can effectively integrate synthetic gene expression with family history, HLA genot

179 The prediction model integrates the synthetic gene expression with other risk factors to ach

180 arate empirical relations between growth and synthetic gene expression.

181 A synthetic gene for chicken ovomucoid first domain (OMCHI

182 A synthetic gene for the human motilin receptor containing

183 ce microscopy of cultured cells expressing a synthetic gene for the receptor.

184 of this approach, we use it to optimize the synthetic genes for 19 repetitive proteins, and show tha

185 We constructed synthetic genes for glucagon-like peptide-1 PODs and dem

186 Synthetic genes for ten completely redesigned proteins w

187 thod of PCR-based gene synthesis, error-free synthetic genes for the human protein kinases PKB2, S6K1

188 oop i3 in G protein coupling, we constructed synthetic genes for the three main D4 receptor variants.

189 assays, plasmids containing codon-optimized synthetic gene fragments (pS plasmids) showed greater th

190 ding to a decrease in the error frequency of synthetic gene from 11.44/kb to 0.46/kb.

191 The ability to mass produce synthetic genes holds great potential for biological res

192 beled rhodopsin, prepared by expression of a synthetic gene in HEK293 cells, was investigated both by

193 cell, BPTI was expressed and secreted from a synthetic gene in the yeast Saccharomyces cerevisiae.

194 r-chloramphenicol acetyltransferase reporter synthetic gene in transient infection assays in neurobla

195 o code for each amino acid, and inserted the synthetic genes in DNA vaccine plasmids.

196 One problem with synthetic genes in genetically engineered organisms is t

197 We found that both natural and synthetic genes in mouse and human cells exhibited an un

198 the expression of IRF7-controlled natural or synthetic genes in several cell lines, including those w

199 her greatly reduce the number of full-length synthetic genes in the library.

200 otential role in the co-expression of starch synthetic genes in the maize endosperm.

201 Analysis identified increases in fatty acid synthetic genes, including Srebp-1 and fatty acid syntha

202 To meet the growing demand for synthetic genes more robust, scalable and inexpensive ge

203 We then expand our method to produce a synthetic gene network acting as a predictable timer, mo

204 We propose a synthetic gene network in Escherichia coli which combine

205 his issue by Cantone et al., who construct a synthetic gene network in yeast and use it to assess and

206 When a synthetic gene network is allowed to regulate a downstre

207 We have tested the new algorithm on the synthetic gene network library bioengineered recently.

208 The parameters in a complex synthetic gene network must be extensively tuned before

209 ize the resilience of phenotypic states in a synthetic gene network near a critical transition.

210 veloped the first mammalian mechanosensitive synthetic gene network to monitor endothelial cell shear

211 The photocaging of a synthetic gene network using unnatural amino acid mutage

212 ecific arrangement of the siRNA targets in a synthetic gene network, allow direct evaluation of any B

213 ble through the design and implementation of synthetic gene networks amenable to mathematical modelli

214 ave important implications for the design of synthetic gene networks and stress that such design must

215 Synthetic gene networks can be constructed to emulate di

216 Here, we show that synthetic gene networks can be engineered to compensate

217 Synthetic gene networks can be used to control gene expr

218 The ability to construct synthetic gene networks enables experimental investigati

219 rug interventions in biomedical contexts and synthetic gene networks for bioengineering.

220 Synthetic gene networks have wide-ranging uses in reprog

221 The construction of synthetic gene networks implementing simple functions ha

222 Synthetic gene networks in mammalian cells are currently

223 ittle over a decade ago with the creation of synthetic gene networks inspired by electrical engineeri

224 The design of synthetic gene networks requires an extensive genetic to

225 and Monod laid the foundation for the first synthetic gene networks that launched the field of synth

226 Efforts to engineer synthetic gene networks that spontaneously produce patte

227 biology has used the engineered assembly of synthetic gene networks to create a wide range of functi

228 We studied its behaviour in synthetic gene networks under increasingly complex condi

229 Synthetic gene networks will also lead to new logical fo

230 Using natural and synthetic gene networks with and without the network mot

231 of gene function and to the construction of synthetic gene networks with desired properties.

232 be able to construct useful next-generation synthetic gene networks with real-world applications in

233 ool for metabolic engineering, the design of synthetic gene networks, and protein manufacturing.

234 and use of antibiotics, the construction of synthetic gene networks, and the development of many cut

235 ents for predicting the behavior of complex, synthetic gene networks, e.g., the whole can be differen

236 literature pertaining to the development of synthetic gene networks, the engineering framework used

237 new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing o

238 the design, construction and modification of synthetic gene networks.

239 a useful tool for designing and controlling synthetic gene networks.

240 for implementing gene expression control in synthetic gene networks.

241 major challenge to engineering sophisticated synthetic gene networks.

242 genetic networks and apply it to a family of synthetic gene networks.

243 urs and also benefits the rational design of synthetic gene networks.

244 netic circuit, a common motif in natural and synthetic gene networks.

245 ne the design, construction and screening of synthetic gene networks.

246 The organization of the fatty acid synthetic genes of Haemophilus influenzae Rd is remarkab

247 In addition, the cobalamin synthetic genes of Salmonella spp. (cob) show a regulato

248 el chromosomal integration and expression of synthetic gene operons in Synechocystis, comprising up t

249 we describe the design and construction of a synthetic gene oscillator in Escherichia coli that maint

250 Like other gene circuits, synthetic gene oscillators are noisy and exhibit fluctua

251 Synthetic gene oscillators are small, engineered genetic

252 Synthetic gene oscillators have the potential to control

253 plied SIFT to identify a set of ultraprecise synthetic gene oscillators, with circuit variants spanni

254 Pyrimidine synthetic genes peaked during DNA replication, and their

255 We further demonstrate the excellence of the synthetic gene products for in vitro mapping of the nucl

256 evealed as mismatches by re-annealing of the synthetic gene products.

257 The synthetic genes provide qualitative controls that can me

258 d, we have successfully constructed numerous synthetic genes, ranging from 139 to 1042 bp.

259 The ability to mass produce these synthetic genes readily will have a significant impact o

260 ng development and aging, and when designing synthetic gene regulation circuits.

261 escribe the development of sequence-targeted synthetic gene regulators (SynGRs) that address these is

262 through rewiring endogenous regulation using synthetic gene regulators based upon CRISPR-Cas.

263 they guide the design of more sophisticated synthetic gene regulators with greater therapeutic poten

264 Analysis of synthetic gene regulatory circuits can provide insight i

265 d can detect causal interactions in specific synthetic gene regulatory circuits in Escherichia coli,

266 s (TALEs) represent attractive components of synthetic gene regulatory circuits, as they can be desig

267 While control over reaction complexity using synthetic gene regulatory networks and DNA nanotechnolog

268 The ability to design and construct synthetic gene regulatory networks offers the prospect o

269 Synthetic gene regulatory networks show significant stoc

270 Importantly, we demonstrate that these synthetic gene-regulatory networks are functional in an

271 al (non-simulated) RNA-seq data from spliced synthetic genes (RNA Sequins) to benchmark its performan

272 while mitigating risks that may emerge, all synthetic gene sequence and synthesis data should be col

273 r, Tyr-66 to His, and Tyr-145 to Phe) with a synthetic gene sequence containing codons preferentially

274 rt the development and characterization of a synthetic gene switch that, when targeted in the mouse g

275 e, and its successful deployment to engineer synthetic gene switches that control and limit Mycoplasm

276 T cells can be controlled photothermally via synthetic gene switches that trigger the expression of t

277 Synthetic gene switches, activated by pharmacologics or

278 y and disassembly is controlled with minimal synthetic gene systems, including an autonomous molecula

279 We have constructed a synthetic gene that encodes for 12 B cell, 6 T cell prol

280 omas expressed a wide range of metabolic and synthetic genes that are expressed during logarithmic gr

281 The pREF sequence encodes 21 synthetic genes that can be in vitro transcribed into sp

282 We have produced two synthetic genes that code for the F2 domain located with

283 When synthetic genes that express RNAs for external guide seq

284 n be controlled by RNA molecules produced by synthetic genes that target the tile interaction domains

285 d yeast was dependent on the availability of synthetic genes that were required to improve translatio

286 ue in conjunction with specifically designed synthetic genes to identify the RS targeted by an inhibi

287 Our tool can predict synonymous codons for synthetic genes toward optimal expression in Escherichia

288 and that 5S rRNA transcripts derived from a synthetic gene transfected transiently into human cells

289 icantly improve transfection efficiencies of synthetic gene vectors.

290 The synthetic gene was cloned into a T7 promoter-controlled

291 A cluster of Bacillus subtilis fatty acid synthetic genes was isolated by complementation of an Es

292 It was reported that the starch synthetic genes were co-expressed during maize endosperm

293 glycoproteins (HRGPs) and HRGPs designed by synthetic genes were consistent with a sequence-driven c

294 uced by Escherichia coli overexpression of a synthetic gene, were reversibly unfolded in 1, 2-dimyris

295 We demonstrated that synthetic genes with tandemly arrayed tRNA-gRNA architec