ライフサイエンスコーパス: target DNA

1 a single transcript, and subsequently cleave target DNA.

2 even mismatches between guide RNA (gRNA) and target DNA.

3 a short downstream PAM sequence to recognize target DNA.

4 ntary sequence to a different portion of the target DNA.

5 rict the density of recognition sequences in target DNA.

6 ructures through specific hybridization with target DNA.

7 oining of newly excised transposon ends with target DNA.

8 t was able to recognize the other end of the target DNA.

9 RVR and RR variants bound to a crRNA and its target DNA.

10 cing site-specific double-stranded breaks in target DNA.

11 dimerization while preventing binding to the target DNA.

12 be, which was complementary with the part of target DNA.

13 orthologs, in complex with an sgRNA and its target DNA.

14 LE and its affinity, for both target and non-target DNA.

15 luster labeled reporter DNA hybridize to the target DNA.

16 in the free protein and in the complex with target DNA.

17 plex formation induce rapid rejection of off-target DNA.

18 by the immobilized probe DNA and hybridized target DNA.

19 he dUTPase activity and Stl release from its target DNA.

20 uations that lead to a slow clearance of the target DNA.

21 aspects of interactions between antigens and target DNA.

22 lts in a staggered seven-nucleotide break of target DNA.

23 a CRISPR RNA (crRNA) is used to detect ASFV target DNA.

24 partially matches a segment (protospacer) in target DNA.

25 the two viral DNA molecules and help capture target DNA.

26 ifically targets and cleaves both strands of target DNA.

27 nsor due to hybridization of the ss-DNA with target DNA.

28 n reaction that restores the RSS (donor) and target DNA.

29 s predominantly a tetramer when bound to its target DNA.

30 and preserved in vitro binding affinity for target DNA.

31 are important for accurate amplification of target DNA.

32 DNA when Cas12a is activated by binding to a target DNA.

33 ty, while PCR offers high specificity toward target DNA.

34 A degradation at double-strand breaks in the target DNA.

35 e signal-on electrochemical detection of the target DNA.

36 A, potentially sequestering the complex from target DNA.

37 ering increased rates of dissociation of off-target DNA.

38 recognizing a specific cis-acting element of target DNA.

39 by four times resulted in 4.4X the yield of target DNA.

40 ng DNA and close again to capture and attack target DNA.

41 e probe DNA is proportional to the number of target DNAs.

42 Rous sarcoma virus in complex with viral and target DNAs.

43 quires systematic profiling across mispaired target DNAs.

44 One system (type I-F) targets DNA.

45 s9 methods aiming to reduce transcription by targeting DNA.

46 nt packed with mega bases of distracting non-target DNA?

47 here was a good linear relationship (F=0.57 [target DNA]+21.31, R(2)=0.9984) between the fluorescent

48 as9 enzymes can tolerate mismatches with the target DNA(3,4).

49 or PAM duplex recognition, as well as blocks target DNA access to key catalytic residues lining the R

50 I/SNF complex subunit composition, chromatin targeting, DNA accessibility and gene expression remain

51 We also find that the conformation of the target DNA after strand transfer is involved in preventi

52 Affinity for non-target DNA also increases non-linearly with the number o

53 were the maceration buffer composition, the target DNA amplicon length, the thermal cycle number and

54 r-friendly, battery-operated and can provide target DNA amplification in less than 30 min.

55 23.0 and 13.2 pM, respectively, without any target DNA amplification.

56 (831 molecules in 35.4 nl assay volume) for target DNA and 16 fM (338 molecules) for target RNA afte

57 n reactions among the FCN-labeled DNA probe, target DNA and capture DNA probe were performed on the l

58 activated only when it specifically binds to target DNA and cleaves it.

59 It reveals severe distortion of target DNA and flipping of the target adenines into extr

60 ion, by both preventing its interaction with target DNA and inducing its rapid degradation by Lon pro

61 orm co-transcriptional cleavage of the viral target DNA and its transcripts.

62 While CRISPR systems have been engineered to target DNA and RNA with increased precision, efficiency,

63 il at the 3' end), for efficient ligation to target DNA and subsequent PCR amplification primed by th

64 nks and additional inter-segment twisting in target DNA and thus attenuates unwanted transposition.

65 vere kinks occur at the integration sites of target DNA and thus prevent the reverse reaction, the sh

66 we investigate the interaction of Cas9 with target DNA and use our findings to improve HDR efficienc

67 he methodology can be suitably used to study targeted DNA and RNA damages from radicals and radiomime

68 sition, how RAG can stabilize sharp bends in target DNA, and why replacement of residue 848 by argini

69 f the Dlx5 homeodomain to recognize and bind target DNAs, and they likely destabilize the formation o

70 n for single nucleotide base-mismatch in the target DNA as well as showed excellent performance to di

71 1) bound to sgRNA as a binary complex and to target DNAs as ternary complexes, thereby capturing cata

72 sCpf1) in complex with the guide RNA and its target DNA at 2.8 A resolution.

73 CdCas9 in complex with the guide RNA and its target DNA at 2.9 angstrom resolution.

74 is ultrasensitive, allowing the detection of target DNA at femtomolar level by simple spectroscopic a

75 The method is capable of detecting target DNA at the concentration down to 0.85 pM in 60min

76 ex with transposon ends covalently joined to target DNA, at resolutions of 3.0-4.6 angstrom.

77 s the direct, irreversible conversion of one target DNA base into another in a programmable manner, w

78 CRISPR/Cas9 genome editing relies on sgRNA-target DNA base pairing and a short downstream PAM seque

79 ated spacer integration requires IHF-induced target DNA bending and explain the elusive role of CRISP

80 e use Mu to investigate the ramifications of target DNA bending on the transposition reaction.

81 Rct) was linear with the number of copies of target DNA between 150 and 10(6) copies/ml.

82 tors, much needs to be learned regarding the target DNA binding by yet-to-be characterized RNPP regul

83 or the biophysical constraints governing off-target DNA binding.

84 e predict optimal nucleotide composition for targeting DNA-binding antibodies.

85 oduct of integration reveals a tetramer with target DNA bound snugly between two dimers in which sing

86 ed atomic force microscopy (AFM) to detect a target DNA bound to small (1.4-1.9 mum diameter) probe D

87 A target DNA-bound structure reveals critical interactions

88 tate, the HNH catalytic state, and a cleaved-target-DNA-bound state.

89 can drive lymphomagenesis by generating off-target DNA breaks at loci that harbor highly active enha

90 approach to study the mechanistic impact of targeted DNA breaks in nearly any chromatin environment.

91 argeted" complexes described herein not only target DNA but also contain either vectors to enable the

92 ly provides the efficient separation for the targeted DNA but can also maintain the bioactivity of as

93 first recognition of a 20-mer portion of the target DNA by a complementary PNA Capture Probe (CP) and

94 toxic and inactive until brought together on target DNA by adjacently bound programmable DNA-binding

95 aled that CM14 inhibits HlyU from binding to target DNA by covalently modifying Cys30.

96 r highly sensitive and specific detection of target DNA by employing the nonlinear hybridization chai

97 It exhibits ratiometric response to the target DNA by FRET acceptor displacement and enables DNA

98 ence of target DNA, the Exo III recycles the target DNA by selectively digesting the dye-tagged seque

99 change of Cas1-Cas2 and protospacer for the target DNA capture.

100 HRP) molecules, recognizing the epimark in a target DNA, captured through hybridization onto streptav

101 ntary base pairing between the guide RNA and target DNA, Cas9-DNA interactions, and associated confor

102 nome engineering, namely the inefficiency of targeted DNA cassette insertion.

103 ng sgRNA design to improve the efficiency of target/DNA cleavage is critical to ensure the success of

104 oped in insects, which leverage the sequence-targeted DNA cleavage activity of CRISPR-Cas9 and endoge

105 tructures in minimizing the formation of off-target DNA complexes.

106 In the first AND gate, a chimeric target DNA comprising of four biomarkers was hybridized

107 Our investigation revealed that a target DNA concentration as low as 5.0x10(-21)M can tran

108 4) between the fluorescent intensity and the target DNA concentration in the samples.

109 ls a linear correlation (R(2) = 0.98) to the target DNA concentration, with a limit of detection down

110 ction-purification process revealed that low target DNA concentrations (80 pg/muL) can be successfull

111 of LbCpf1 in complex with the crRNA and its target DNA containing either TTTA, TCTA, TCCA, or CCCA a

112 asis for improved discrimination against off-target DNA containing important mismatches at the distal

113 netics at physiological temperatures and for target DNAs containing multiple, adjacent binding sites.

114 Target DNA could be detected with the lower limit of qua

115 allow for proper control of toxicity from on-target DNA damage.

116 CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target sites

117 of immunotherapy with pembrolizumab or drugs targeting DNA damage, such as olaparib, might be used to

118 These findings establish a mechanism for co-targeting DNA damage-induced cell cycle checkpoints in c

119 Analysis of a HIF-3 target, DNA-damage-inducible transcript 4, a key surviva

120 nferred a therapeutic vulnerability to drugs targeting DNA-damage repair pathways.

121 a 45-fold overall reduction in unguided off-target DNA deamination relative to BE4 containing rAPOBE

122 f Cas9-gRNA binding to fluorescently labeled target DNA derivatives called "Cas9 beacons." We observe

123 al amplification (LAMP) as the chemistry for targeted DNA detection, by virtue of its high sensitivit

124 onto compact discs is proposed for real-time targeted DNA determination.

125 dues W159, R186, F187 and K190 stabilise the target DNA distortions and are required for efficient tr

126 nal change upon hairpin hybridization to the target DNA, dominated by the "on-off" signal change mode

127 ociated protein (CRISPR/Cas) system, used to target DNA double-strand breaks (DSBs).

128 Using targeted DNA double-strand breaks (DSBs) and long-read w

129 Targeted DNA double-strand breaks have been shown to sig

130 ith Probe-based DNA Enrichment by RNA probes targeting DNA duplex (DEEPER-Seq).

131 Restriction endonucleases naturally target DNA duplexes.

132 These variants also showed more precise on-target DNA editing than the wild-type CBE and, for most

133 -100-fold lower average Cas9-independent off-target DNA editing while maintaining robust on-target ed

134 arget RNA-editing activity and comparable on-target DNA-editing activity that are also among the smal

135 the specificity of CBEs have identified off-target DNA edits in mammalian cells(5,6).

136 In the presence of target DNA, FCNs were captured on the test zone of the b

137 In the presence of target DNA, formation of the anionic DNA-acpcPNA duplex

138 PCR at a lower concentration to amplify its target DNA fragment.

139 agnetic bead-based method for the capture of target DNA from a pool of interfering genomic DNA.

140 exponentially amplifying very low amounts of target DNA from genetic, clinical, and forensic samples.

141 from 1 pM to 1000 pM, and could discriminate target DNA from mismatched sequences.

142 cies-specificity as they did not amplify non-target DNAs from closely related filarial species, human

143 lones are broad-spectrum antibacterials that target DNA gyrase by stabilizing DNA-cleavage complexes,

144 nd aminocoumarin antibiotics, compounds that target DNA gyrase in bacteria.

145 ied a class of antibacterial thiophenes that target DNA gyrase with a unique mechanism of action and

146 Fluoroquinolone antibacterials, which target DNA gyrase, are critical agents used to halt the

147 We identified over 40 compounds that target DNA gyrase, the cell wall, tryptophan, folate bio

148 An important antibiotic target, DNA gyrase is an essential bacterial enzyme that

149 ature of the platform, detection of multiple target DNAs has been successfully demonstrated.

150 facilitating zippering up of 20-bp guide RNA:target DNA heteroduplex on ternary complex formation.

151 tance (R(CT)) changes induced by the aptamer-target DNA hybridization and that caused by the drift co

152 to form stable interactions with their model target DNA in cellulo, whereas the third one loses this

153 s been successfully applied for detection of target DNA in complex sample matrices.

154 uctures of Cas1-Cas2 bound to both donor and target DNA in intermediate and product integration compl

155 specific and identify down to 0.1% of their target DNA in meat mixtures.

156 Furthermore, we demonstrated that target DNA in real human plasma samples can be directly

157 DNA biosensor was demonstrated by detecting target DNA in spiked serum samples.

158 uirements for cleavage of the two strands of target DNA in vitro.

159 s resistant to many immunity mechanisms that target DNA in vivo, including two subtypes of CRISPR-Cas

160 zebrafish, improving current approaches for targeted DNA integration in the genome.

161 yotic or eukaryotic cells and integrates the target DNA into the genome.

162 The target DNA is a T form (T for transpositional target), w

163 additional operation steps, an LOD of 15 fM target DNA is achieved with a total assay time of ca. 10

164 pacer adjacent motif (PAM) sequence flanking target DNA is crucial for self versus foreign discrimina

165 In the strand transfer structure, target DNA is severely bent and the TTAA target is unpai

166 of length on affinity for target versus non-target DNA manifests in specificity increasing then dimi

167 -damage response and sensitizes Mtb to drugs targeting DNA metabolism and respiration.

168 Using chemical biomarkers and a targeted DNA metagenomic methodology, we show that E. af

169 one accession move across a graft union and target DNA methylation de novo at normally unmethylated

170 pendent forms of RdDM function to critically target DNA methylation to full-length and transcriptiona

171 ngineered DNA methyltransferases (MTases) to target DNA methylation to specific sites in the genome w

172 n and minimize the unintended effects of off-target DNA methylation.

173 facile and convenient approach for efficient targeted DNA methylation by fusing inactive Cas9 (dCas9)

174 catalytically inactive Cas9 (dCas9) enables targeted DNA methylation editing.

175 HARDEN allows for higher targeted DNA methylation levels than a dCas9-DNMT3a fusi

176 We perform targeted DNA methylation profiling for a diverse panel o

177 We conducted targeted DNA methylation sequencing to identify DNA meth

178 etic eNgineering (HARDEN), a novel method of targeted DNA methylation that utilizes endogenous DNA do

179 This method allows for stable targeted DNA methylation through the process of homology

180 nesis, RNA polymerase V (Pol V) recruitment, targeted DNA methylation, and gene-expression changes at

181 trategies for epigenetic gene regulation via targeted DNA methylation.

182 which natural variation in the robustness of targeting DNA methylation to heterochromatin exists, and

183 cum DRM methyltransferase, which efficiently targets DNA methylation to specific loci, including the

184 l RNA production, and the cell more strongly targets DNA methylation to TEs that have the potential t

185 g development is still largely restricted to target DNA methylome, emerging evidence indicates that h

186 rating cells can be altered by oxidants that target DNA methyltransferase activity or deplete its sub

187 associated with reduced activity of the E2F target, DNA methyltransferase 1.

188 hosphorylation and acetylation of STAT3 that targeted DNA methyltransferase 1 (DNMT1) in a sequential

189 ysi)(phen)(PPO)]Cl(2) (Rh-PPO), specifically target DNA mismatches and selectively induce cytotoxicit

190 s induces meristems that produce shoots with targeted DNA modifications, and gene edits are transmitt

191 P primers can be used to quantify and detect target DNA molecules down to single copy numbers.

192 gle-DNA molecule/TIRS nanotag hybridization, target DNA molecules of H7N9 were detected down to 74 zM

193 tool in this regard; its ability to recycle target DNA molecules results in markedly improved detect

194 ishing perfect-matched and single-mismatched target DNA molecules to the best extent, likely due to t

195 PCR system provides absolute quantitation of target DNA molecules using fluorescent dual-labelled pro

196 L initiative assessed cases by whole-genome, targeted DNA, mRNA and microRNA sequencing and CpG methy

197 em is emerging as a robust biotechnology for targeted-DNA mutation.

198 EA, using light-induced release of DHEA from targeted DNA nanocapsules.

199 By leveraging this technique, target DNA of Sus scrofa (pork) meat was detected as low

200 (MQDS) was developed to identify and measure target DNAs of pathogenic microorganisms and eliminated

201 rug resistance to platinum chemotherapeutics targeting DNA often involves abrogation of apoptosis and

202 Enhancing the flexibility of the target DNA or prebending it increases its affinity for t

203 e biosensor had good selectivity towards the target DNA over the non-specific sequences and also it w

204 The effect of a target DNA overhang on the hybridization efficiency was

205 A longer target DNA overhang was found to provide a better respon

206 In addition to targeting DNA, oxidative stress can affect proteins like

207 The target DNA partly hybridizes with capture probe on the g

208 engineering by facilitating a wide range of targeted DNA perturbations.

209 ex with transposon ends covalently joined to target DNA, portrays the transposition machinery after D

210 d samples provided similar information about target DNA presence.

211 able flap can then be actuated by a specific target DNA present in a sample, by exposing a hemin/G-qu

212 AG engaged with transposon ends and U-shaped target DNA prior to integration (the target capture comp

213 The molecular basis of Rgg-SHP and Rgg-target DNA promoter specificity was unknown.

214 o adopt a single conformation upon binding a target DNA promoter.

215 d S. thermophilus Rgg3 in complex with their target DNA promoters.

216 o biomaterial analyses on increasing scales, targeting DNA, proteins, cells, and tissues.

217 leanup performance between 91.9 and 99.9% of target DNA reads and successful amplification of all tar

218 utline our state-of-the-art understanding of target DNA recognition and cleavage by CRISPR-Cas9 nucle

219 Therapeutic strategies targeting DNA repair pathway defects have been widely ex

220 examine the development of selective agents targeting DNA repair, including PARP inhibitors; inhibit

221 miR-155 is known to target DNA-repair proteins, leading to a mutator phenoty

222 DNA repair templates results in precise and targeted DNA replacement with as much as approximately 1

223 Thus targeting DNA replication and G2-M cell cycle checkpoint

224 of Ca1-Cas2 to the protospacer and potential target DNAs respectively.

225 of the reaction in the presence of the Cas9 target-DNA revealed a delay between first and second str

226 LOF methods target DNA, RNA or protein to reduce or to ablate gene f

227 ntaining up to four mismatches in the Cas12a-targeted DNA-RNA hybrid sequences.

228 a 35-fold mass amplification by an antibody targeting DNA-RNA hybrids and polyclonal secondary antib

229 mediately "activated" and enabled to capture target DNA/RNA efficiently from the opposite side of the

230 rase together with the chimeric nucleotides, target DNAs/RNAs trigger the release of stoichiometrical

231 sis of metal complexes that can specifically target DNA secondary structure has attracted considerabl

232 Targeting DNA secondary structures thus represents a pot

233 ansfer of the recessed 3'-hydroxyls into the target DNA separated by 4-6 bp.

234 udied tumor suppressor gene, was used as the target DNA sequence model.

235 ould exclusively and specifically detect the target DNA sequence of B. cereus from other bacteria tha

236 Titration in the presence of the Egr1 target DNA sequence supports binding to GC bases as repo

237 provides high sensitivities for a synthetic target DNA sequence with a unique 5-hmC in the promoter

238 In the presence of the complementary target DNA sequence, the probes will compete for binding

239 ains of MeCP2 in vitro and defined a minimal target DNA sequence.

240 he search of the DNA-binding species for its target DNA sequence.

241 facilitate rapid bacterial identification by targeted DNA sequence analysis or by whole-genome sequen

242 A (sgRNA) directs the endonuclease Cas9 to a targeted DNA sequence for site-specific manipulation.

243 becomes arrested immediately upstream of the targeted DNA sequence, and is not rescued by transcripti

244 tain unbiased and accurate nanopore data for target DNA sequences <100 bp.

245 Large-scale mutagenesis of target DNA sequences allows researchers to comprehensive

246 face to facilitate the selection of the best target DNA sequences for experimental design.

247 as Cas9 and Cas12, have been widely used to target DNA sequences in eukaryotic genomes.

248 ns bearing missense mutations failed to bind target DNA sequences on EMSA and confocal microscopy; ho

249 kable (down to aM) detection sensitivity for target DNA sequences present in solution.

250 otein binding and nuclease activation at off-target DNA sequences remains elusive.

251 eproducibly analyzed as few as 100 copies of target DNA sequences using gold/silver nanostars, thus d

252 ientations at a fixed distance downstream of target DNA sequences, and can accommodate variable lengt

253 d zinc finger domains involved in binding to target DNA sequences.

254 tens of thousands of CRISPR RNA (crRNA) and target DNA sequences.

255 ance to discriminate closely related six non-target DNA sequences.

256 in vivo and enables continuous evolution of targeted DNA sequences.

257 r somatic single-nucleotide variants by deep-targeted DNA sequencing and for chromosomal allelic imba

258 Exome sequence analysis with validation by targeted DNA sequencing of 125 samples uncovered, in add

259 Here, Patel et al. use targeted DNA sequencing of MODY patients and large-scale

260 Here, we performed deep targeted DNA sequencing of multiple metastases from mela

261 Targeted DNA sequencing showed the mouse BPRN tumors, bu

262 Targeted DNA sequencing was performed on 243 (34.9%) pat

263 insertion sites are typically identified by targeted DNA-sequencing and subsequently assigned to pre

264 Targeted DNA-sequencing was performed on 26 of 36 biopsi

265 tide (brHis2) does not bind to its consensus target DNA site (5'-GTCAT-3').

266 spacer acquisition from the vicinity of the target DNA site cleaved by Cas9.

267 Rgamma:RXRalpha heterodimer to more than 300 target DNA sites and variants thereof.

268 2) Non-target DNA sites have a significant effect on search kin

269 by transcription factors binding to specific target DNA sites.

270 gene expression through binding to specific target DNA sites.

271 The target DNA spiked food matrix (chicken meat) is also suc

272 tively occupies both PAM-interacting and non-target DNA strand cleavage catalytic pockets.

273 tion the HNH nuclease domain adjacent to the target DNA strand cleavage site in a conformation essent

274 d-type BhCas12b preferentially nicks the non-target DNA strand instead of forming a double strand bre

275 with wedge insertion, initiating directional target DNA strand unwinding to allow segmented base-pair

276 DNA for complementary base-pairing with the target DNA strand while displacing the non-target strand

277 me process also generates a bulge in the non-target DNA strand, enabling its handover to Cas3 for cle

278 te of Cas9 HNH domain primed for cutting the target DNA strand.

279 leotide sequences in both the target and non-target DNA strands and recognizes the 5'-NNNVRYM-3' as t

280 rmations of AacC2c1 with both target and non-target DNA strands independently positioned within a sin

281 to screen a number of labeled and unlabeled target DNA strands to measure the impact of fluorescent

282 their respective viral DNA or branched viral/target DNA substrates have indicated these intasomes are

283 ns conferred resistance; most of these drugs target DNA synthesis or topoisomerase and cause DNA dama

284 In the presence of target DNAs (T-DNA), hairpin probes hybridized with T-DN

285 DNA (vDNA) copy of its RNA genome into host target DNA (tDNA).

286 In the presence of target DNA, the Exo III recycles the target DNA by selec

287 After binding the nanoparticles with the target DNA, the following sandwich structure was formed:

288 demonstrated that the genomic context of the targeted DNA, the GC percentage, and the secondary struc

289 Small molecules that target DNA to interfere with protein-DNA interactions ma

290 atic cleavage of the RNA probe, allowing the target DNA to liberate and hybridize with another RNA pr

291 tes single-mismatched DNA from fully matched target DNA under optimal conditions.

292 The system specifically amplifies the target DNA using loop-mediated isothermal amplification

293 ichia coli and Listeria monocytogenes, which target DNA via a multi-component RNA-guided complex term

294 ted to cleave both target RNA (via Cas7) and target DNA (via Cas10).

295 s, the on-chip quantitative detection of the target DNA was readily achieved using an inexpensive the

296 lyses the integration of viral DNA into host target DNA, which is an essential step in the life cycle

297 errogation steps that precede the pairing of target DNA with guide RNA.

298 he probe, and the signal was compared to the target DNAs with different lengths and overhangs.

299 repeat provides extra binding energy for the target DNA, with the gain decaying exponentially such th

300 ld sensing chip and the unpaired fragment of target DNA works as a trigger to initiate the nonlinear