ライフサイエンスコーパス: taste cell

1 re expressed preferentially in T1r3-positive taste cells.

2 rtion of K(+) channels in the T1r3 subset of taste cells.

3 ed changes in C(m) in the different types of taste cells.

4 of which is transduced by a separate set of taste cells.

5 on conductance specific to PKD2L1-expressing taste cells.

6 ting cAMP alters Ca(2+) levels in identified taste cells.

7 ngs food-related chemicals into contact with taste cells.

8 of Ca(2+) in the apical tips of a subset of taste cells.

9 s as well as physiologic studies of isolated taste cells.

10 otassium exchangers (NCKXs) are expressed in taste cells.

11 s with the signalling mechanisms used by the taste cells.

12 s with the signalling mechanisms used by the taste cells.

13 localized to the apical tips of a subset of taste cells.

14 receptors expressed in different subsets of taste cells.

15 le one of these, ROMK2, in a subset of mouse taste cells.

16 secreted from isolated mouse taste buds and taste cells.

17 in-1-LIR are present in different subsets of taste cells.

18 e qualities have been recorded in individual taste cells.

19 receptor signaling and membrane potential in taste cells.

20 o acid decarboxylase (AADC) are expressed in taste cells.

21 dense-cored vesicles previously seen in some taste cells.

22 n detected immunocytochemically in mammalian taste cells.

23 xplain the taste responses observed in mouse taste cells.

24 d in taste tissue and in Ggamma13-expressing taste cells.

25 gal) and trkB colocalize, mainly in type III taste cells.

26 trophin (NT)-3- nor trkC-LIR was detected in taste cells.

27 m5) and type III (e.g. Pkd2l1, Ncam, Snap25) taste cells.

28 of electrophysiological studies performed on taste cells.

29 eptor (IP3R3), a taste-signaling molecule in taste cells.

30 ific G-protein-coupled membrane receptors on taste cells.

31 esent in differentiated type II and type III taste cells.

32 t and receive synaptic contact from Type III taste cells.

33 changes in pH and [Ca2+]i simultaneously in taste cells.

34 th blood group H antigen, a marker of type I taste cells.

35 to be detected by T1r receptors expressed in taste cells.

36 esponsive receptors and/or pathways exist in taste cells.

37 n-induced Ca2+ entry into a select subset of taste cells.

38 ong slender taste cells, as well as pyriform taste cells.

39 different subpopulations of bitter-sensitive taste cells.

40 y compose a subpopulation of acid-responsive taste cells.

41 sm contributes to salt responses in type III taste cells.

42 ls compose subpopulations of acid-responsive taste cells.

43 iosynthesizing enzyme of endocannabinoids in taste cells.

44 lycerol lipase alpha (DAGLalpha)) of 2-AG in taste cells.

45 vation of Y2 receptors localized apically in taste cells.

46 activation of its high affinity receptor in taste cells.

47 eoside did not affect Presynaptic (type III) taste cells.

48 s calcium mobilization in Receptor (Type II) taste cells.

49 to the postnatal expression of gustducin in taste cells.

50 nel (Kir) 6.1] were expressed selectively in taste cells.

51 owed that a subset of Presynaptic (Type III) taste cells (53%) responded to 0.1 mum CGRP with an incr

52 substrates of taste behaviors, we monitored taste cell activity in vivo with the genetically encoded

53 lace at the microvilli on the apical side of taste cells after diffusion of the molecules through the

54 lecular receptive range (MRR); some of these taste cells also contain two signaling pathways with dis

55 the overwhelming majority of T1r3-expressing taste cells also express SUR1, and vice versa, it is lik

56 using a reporter mouse strain, we show that taste cells also express the anti-inflammatory cytokine

57 Here, we tested the contribution of Otop1 to taste cell and gustatory nerve responses to acids in mic

58 tion involves communication between Type III taste cells and 5-HT3 -expressing afferent nerve fibers

59 y longer and narrower than FITBs, with fewer taste cells and a smaller nerve plexus.

60 urotransmitters to communicate with adjacent taste cells and afferent nerve fibers.

61 g, we identified AI salt-responsive type III taste cells and demonstrated that they compose a subpopu

62 , specifically free fatty acids, to activate taste cells and elicit behavioral responses consistent w

63 acid, linoleic acid (LA), depolarizes mouse taste cells and elicits a robust intracellular calcium r

64 e and suggests subsequent synaptic spread to taste cells and epithelial cells via peripheral synapses

65 addition, the immunocytochemical profile of taste cells and gustatory behavior were examined in wild

66 involved in sugar and amino acid sensing in taste cells and in the gastrointestinal tract.

67 in the apical membrane of PKD2L1-expressing taste cells and is not affected by targeted deletion of

68 s well as by GAD67 in presynaptic (type III) taste cells and is stored in both those two cell types.

69 In taste cells and macrophages, CD36 signaling was recently

70 The ultrastructure of contacts between taste cells and nerve fibers is also normal in the DKO m

71 we did not observe synapses between type II taste cells and nerve fibers.

72 Synaptic contacts between taste cells and nerve processes have been found to exist

73 tions between the plasma membrane of type II taste cells and nerve processes.

74 as secreted only from presynaptic (type III) taste cells and not receptor (type II) cells.

75 heterogeneous population of bitter-sensitive taste cells and signaling pathways within this insect fa

76 s (SCCs) have a structure similar to lingual taste cells and strongly express alpha-gustducin.

77 ial role in fatty acid transduction in mouse taste cells and suggest that fatty acids are capable of

78 is required for generation of differentiated taste cells and taste bud maintenance.

79 ncreases in intracellular Ca(2+) in isolated taste cells and that the source of the Ca(2+) is release

80 ATP release channel CALHM1/3 in a subset of taste cells and that these cells mediate amiloride-sensi

81 is required for generation of differentiated taste cells and that, in the absence of R-spondin in cul

82 ecific transfer of taste information between taste cells and the central nervous system.

83 citatory responses from the bitter-sensitive taste cells and then used these responses to formulate p

84 Using calcium imaging on single isolated taste cells and with biosensor cells to identify neurotr

85 markedly reduced cytokeratin 8 expression in taste cells, and a high incidence of a filiform-like spi

86 bud placodes, fungiform papillae, and mature taste cells, and low levels in filiform papillae.

87 nsory neurons, solitary chemoreceptor cells, taste cells, and Merkel cells.

88 evalence of poorly differentiated or missing taste cells, and the incidence of ectopic filiform-like

89 Taste cells are continuously renewed throughout life via

90 Moreover, they show that individual taste cells are dedicated to the transduction of only on

91 the synapses associated with syntaxin-1-LIR taste cells are from type III cells onto nerve processes

92 transgenic mice in which PLCbeta2-expressing taste cells are labeled with green fluorescent protein.

93 As placodally derived taste cells are lost, we used Wnt1Cre mice to show that

94 B-LIR, and p75-LIR elongated, differentiated taste cells are present within all lingual taste buds, w

95 within taste buds, and like epidermal cells, taste cells are regularly replaced throughout adult life

96 bpopulation of serotonin-immunoreactive (IR) taste cells are related by lineage to a restricted set o

97 These studies demonstrate that taste cells are selectively tuned to different taste mod

98 These studies demonstrate that taste cells are tuned by taste category and are hardwire

99 ng pathways within the same bitter-sensitive taste cell (aristolochic acid).

100 buds: GABA is synthesized by GAD65 in type I taste cells as well as by GAD67 in presynaptic (type III

101 , beta-gal (BDNF) is present in long slender taste cells, as well as pyriform taste cells.

102 ence of a reduced number of bitter-activated taste cells, as well as reduced sensitivity.

103 ify genes that were selectively expressed in taste cells at different maturation stages.

104 urs in anteriorly placed taste buds, however taste cells at the back of the tongue respond to umami c

105 ype I (blood group H antigen immunoreactive) taste cells but is present in differentiated type II and

106 cocious differentiation of Type I glial-like taste cells, but not other taste cell types.

107 t to gate TRPM5-dependent currents in intact taste cells, but only intracellular Ca2+ is able to acti

108 We recorded activation of taste cells by bitter stimuli using Ca2+ imaging in ling

109 e buds without affecting the excitability of taste cells by taste stimuli.

110 y, acid sensitivity is not conferred on sour taste cells by the specific expression of Kir2.1, but by

111 ing pathway within the same bitter-sensitive taste cell (caffeine).

112 In response to inflammatory challenges, taste cells can increase IL-10 expression both in vivo a

113 Similarly, Type II taste cells come in at least two varieties: those immuno

114 ificantly larger and have a larger number of taste cells compared with controls.

115 We isolated mouse taste buds and taste cells, conducted functional imaging using Fura-2,

116 This communication between taste cells could represent a potential convergence of t

117 data suggest a model where continued natural taste cell death, paired with temporary interruption of

118 rus can promote generation of differentiated taste cells despite denervation.

119 In bdnf(-/-) mice taste cell development failed because of sparse gustator

120 ts taste perception without interfering with taste cell development or integrity.

121 ntify multiple signaling pathways underlying taste cell differentiation and taste stem/progenitor cel

122 to sour taste sensing by regulating type III taste cell differentiation in mice.

123 Strikingly, placodally descendant taste cells disappear early in adult life.

124 cal features, that both type II and type III taste cells display synaptobrevin-2-LIR.

125 e populations of AI salt-responsive type III taste cells distinguished by their sensitivity to anion

126 approximately 27% of the synaptobrevin-2-LIR taste cells do not display IP3R3-LIR.

127 uggesting that ACh is normally released from taste cells during taste stimulation.

128 croscopy also showed the calcium activity of taste cells elicited by small-sized tastants in the bloo

129 on of the whole taste bud, only a select few taste cells exhibited Ca2+ responses.

130 nscription (RT)-PCR, we show that individual taste cells express either phospholipase C beta2 (PLCbet

131 aste; our starting point was to determine if taste cells express glucose transporters (GLUTs) and met

132 All IP3R3-LIR taste cells express synaptobrevin-2-LIR.

133 These taste cells express the epithelial sodium channel ENaC,

134 ning showed that nearly all rodent and human taste cells express this channel.

135 Alpha-gustducin, a taste cell-expressed G-protein alpha subunit closely rel

136 Taste cell-expressed glucose sensors and K(ATP) may serv

137 A recent study showed that taste cells expressing bitter-responsive taste receptors

138 nt protein, we previously reported that sour taste cells from circumvallate papillae in the posterior

139 from DKO mice, as from wild-type (WT) mice, taste cells from DKO mice fail to release ATP when stimu

140 nion sodium salts, respectively) in isolated taste cells from mouse circumvallate papillae.

141 the current is present in PKD2L1-expressing taste cells from mouse circumvallate, foliate, and fungi

142 ells isolated from TRPM5 knockout mice or in taste cells from wild type mice where current through TR

143 This demonstrates a direct taste cell function for BDNF.

144 however, the molecular mechanisms governing taste cell generation are not well understood.

145 1) different populations of bitter-sensitive taste cells (Grindelia extract and Canna extract) or (2)

146 Each bilateral pair of bitter-sensitive taste cells has a different molecular receptive range (M

147 proton conductance that is specific to sour taste cells has been shown to be the initial event in so

148 ha-gustducin in bitter taste transduction in taste cells has not been demonstrated in situ at the cel

149 els encoded by the TRPM5 gene, we found that taste cells have a second type of Ca2+-activated nonsele

150 The serotonin-immunoreactive taste cells have an invaginated nucleus, synaptic contac

151 Gustatory receptors and peripheral taste cells have been identified in flies and mammals, r

152 Notably, mature taste cells have life spans of only 5-20 days and, conse

153 expressing these molecules are distinct from taste cells having receptors for bitter, sweet, or umami

154 NGF, pro-NGF, and trkA coexist in type II taste cells, i.e., those expressing phospholipase Cbeta2

155 t that fatty acids are capable of activating taste cells in a manner consistent with other GPCR-media

156 hypothesis of purinergic signalling between taste cells in a more integral preparation.

157 we undertook an immunohistochemical study of taste cells in BDNF(LacZ) gene targeted "knock-in" adult

158 ses in cells isolated from taste buds and in taste cells in lingual slices to acetic acid titrated to

159 We show that PYY is expressed in subsets of taste cells in murine taste buds.

160 aste qualities by distinct subpopulations of taste cells in peripheral gustatory sensory organs in mi

161 -LIR) is present in approximately 35% of the taste cells in rat circumvallate taste buds.

162 sent in the morphologically defined Type III taste cells in rats.

163 act tongue mucosa and functional activity of taste cells in response to topically administered tastan

164 Cell lineage analysis of serotonin-IR taste cells in such mixed taste buds suggests that this

165 haracteristics and interrelationships of the taste cells in the circumvallate papillae of adult mice.

166 Whereas 82% of the taste cells in the clOFC respond to glucose, in the mOFC

167 f carbonation as well as the contribution of taste cells in the orosensory response to CO2.

168 To examine the role of PKD2L1-expressing taste cells in vivo, we engineered mice with targeted ge

169 Activation of these taste cells, in turn, engages neural circuits in the cen

170 the mechanisms underlying integration of new taste cells into the taste bud.

171 hological taste cell types, but the type III taste cell is the only cell type that has synapses onto

172 is constitutively open, the cytosol of sour taste cells is acidified.

173 In this study, we use taste cells isolated from mouse vallate and foliate papi

174 Nevertheless, some taste cells lacking alpha-gustducin responded to bitter

175 s reveal that acids activate a unique set of taste cells largely dedicated to sour taste, and they in

176 Moreover, siRNA knockdown of WT1 in cultured taste cells leads to a reduction in the expression of Le

177 ning with antibodies against type II and III taste cell markers validated the presence of KCNQ1 in th

178 multiple progenitors, the different types of taste cells may represent distinct lineages.

179 Modulating GLP-1 secretion in gut "taste cells" may provide an important treatment for obes

180 Modulating hormone secretion from gut "taste cells" may provide novel treatments for obesity, d

181 In contrast, pharyngeal taste cells mediate the egg-laying attraction to lobelin

182 roton conductance to directly depolarize the taste cell membrane.

183 onium, which interacts with K(+) channels in taste cells, most likely binds to and blocks Kir6.2.

184 ors are specified and produce differentiated taste cells normally, in the absence of gustatory nerve

185 ease after development but continues in some taste cells of adult mice.

186 The identification in sour taste cells of an acid-sensitive K(+) channel suggests a

187 aled significantly stronger BDNF labeling in taste cells of high BDNF-expressing mouse lines compared

188 e responses to umami tastants persist in the taste cells of T1R3-knockout mice.

189 rs (VPAC1, VPAC2) were identified in type II taste cells of the taste bud, and VIP knockout mice exhi

190 se structures in the tongue, neuroepithelial taste cells of the taste bud, and, possibly, epithelial

191 glucose through the same mechanisms used by taste cells of the tongue.

192 on and that sugar-sensing inhibition affects taste cells on the proboscis and the legs.

193 Sour taste is detected by a subset of taste cells on the tongue and palate epithelium that res

194 could underlie AI salt responses in type III taste cells, one of which may contribute to the anion ef

195 mediated by three pairs of bitter-sensitive taste cells: one responds vigorously to aristolochic aci

196 All of the synapses that we observed from taste cells onto nerve processes express synaptobrevin-2

197 ggest that epithelial cells, neuroepithelial taste cells, or olfactory sensory neurons at chemosensor

198 Sweet taste cells play critical roles in food selection and fe

199 Previous studies have identified one taste cell population marked by the gustatory receptor g

200 p75-LIR also is present in both BDNF and NGF taste cell populations.

201 Taste cells possess vesicles containing various neurotra

202 To determine which types of taste cells produce BDNF, we undertook an immunohistoche

203 and Hh signaling pathways are necessary for taste cell proliferation, differentiation and cell fate

204 e data define a functional signature for the taste cell proton current and indicate that its expressi

205 In contrast, Receptor (Type II) taste cells rarely (4%) responded to 0.1 mum CGRP.

206 o molecularly distinct functional classes of taste cells: receptor cells and synapse-forming cells.

207 ry and inhibitory effects often differs when taste cell recording changes from the NST to the PBN.

208 Type III taste cells release 5-HT directly in response to acidic

209 In response to taste stimuli, taste cells release ATP, activating purinergic receptors

210 d on cell processes that often envelop other taste cells, reminiscent of type I cells.

211 te radiation injury and/or speed recovery of taste cell renewal following fractionated IR.

212 is in part responsible for the dependence of taste cell renewal on gustatory innervation, neurotrophi

213 de a local supply of Hh ligand that supports taste cell renewal.

214 re used to discriminate type II and type III taste cells, respectively.

215 onin (5HT) as markers of type I, II, and III taste cells, respectively.

216 For instance, whether and how individual taste cells respond to multiple chemical stimuli is stil

217 Calcium imaging revealed that isolated taste cells responded with a transient elevation of cyto

218 onded to multiple taste qualities, with some taste cells responding to both appetitive ("sweet") and

219 T1R3 as a coreceptor in taste cells responds to sweet compounds and amino acids;

220 Inosine monophosphate enhanced taste cell responses to L-glutamate, a characteristic fe

221 tively inhibits behavioural, taste nerve and taste cell responses to sweet compounds.

222 Importantly, taste cell responses were not inhibited by the diuretic,

223 Approximately 5% of taste cells selectively responded to L-glutamate when it

224 identifying their patterns of expression in taste cells sheds light on coding of taste information b

225 VIP knockout mouse taste cells show a significant decrease in leptin recept

226 The results demonstrate that only type III taste cells show significant depolarization-induced incr

227 TrkB transcripts in taste buds and elevated taste cell-specific TrkB phosphorylation in response to

228 TPs, many (66%) AI salt-responsive type III taste cells still exhibited the anion effect, demonstrat

229 comprehensive map of gene expression for all taste cell subpopulations and will be particularly relev

230 localization suggest the presence of several taste cell subtypes.

231 These results support the proposition that taste cell synapses use classical SNARE machinery such a

232 We hypothesize that taste cell synapses utilize the SNARE protein machinery

233 ol synaptic vesicle fusion and exocytosis at taste cell synapses.

234 is associated with synaptic vesicles at rat taste cell synapses.

235 icle docking and neurotransmitter release at taste cell synapses.

236 ary, we postulate that aminergic presynaptic taste cells synthesize only 5-HT, whereas NE (perhaps se

237 sion in two distinct subpopulations of mouse taste cells: Tas1r3-expressing type II cells and physiol

238 Isolated taste cells, taste buds and strips of lingual tissue fro

239 salty, sour, umami) are sensed by dedicated taste cells (TCs) that relay quality information to gust

240 Z) mice indicate that BDNF is not present in taste cells that are younger than 3 days postmitotic.

241 In Drosophila, taste cells that contain the Gr5a receptor are necessary

242 ession is mostly restricted to the subset of taste cells that detect sour.

243 and L-amino acids is exclusively mediated by taste cells that express one or pair-wise combinations o

244 blished that sour is detected by a subset of taste cells that express the TRP channel PKD2L1 and its

245 cAMP and Ca(2+) signalling in a subclass of taste cells that form synapses with gustatory fibres and

246 hannels and calcium regulatory mechanisms in taste cells that functions to keep cytosolic calcium lev

247 that a constitutive calcium influx exists in taste cells that is regulated by mitochondrial calcium t

248 t has approximately eight bilateral pairs of taste cells that respond selectively to bitter taste sti

249 We identify taste cells that respond to NaCl in the presence of amil

250 In taste cells that responded to multiple stimuli, there we

251 not well understood, and the identity of the taste cells that secrete NE is not known.

252 e information in the taste bud, resulting in taste cells that would respond broadly to multiple taste

253 s review discusses the functional classes of taste cells, their cell biology, and current thinking on

254 Taste buds comprise four types of taste cells: three mature, elongate types, Types I-III;

255 llular stores while other stimuli depolarize taste cells to cause calcium influx through voltage-gate

256 hat extends from nasal epithelia and type II taste cells to ex-Aire-expressing medullary thymic cells

257 ough the apical pore, and allowing excitable taste cells to maintain a hyperpolarized resting membran

258 ATP further stimulates other taste cells to release a second transmitter, serotonin.

259 amines in taste, we evaluated the ability of taste cells to synthesize, transport, and package 5-HT a

260 The classic type II taste cell transcription factor POU2F3 is lineage defini

261 How taste cells transduce sour taste is controversial becaus

262 In taste cells, Trpm5 was coexpressed with taste-signaling

263 taste bud, and resolve the paradox of broad taste cell tuning despite mutually exclusive receptor ex

264 cellular and molecular mechanisms governing taste cell turnover.

265 Which taste cell type(s) (Type I/glial-like cells, Type II/rec

266 ma receptor IFNGR1, are coexpressed with the taste cell-type markers neuronal cell adhesion molecule

267 nts in the three principal elongate types of taste cells: type I, II, and III.

268 To determine which of the three taste cell types expresses this enzyme, double-label ass

269 unohistochemistry using markers of different taste cell types in brain-derived neurotrophic factor (B

270 To identify the taste cell types that express proteins involved in PLC s

271 ch give rise to at least two different adult taste cell types, but do not contribute to taste papilla

272 lian buds contain a variety of morphological taste cell types, but the type III taste cell is the onl

273 The ectopic buds are composed of all taste cell types, including support cells and detectors

274 To identify taste cell types, mice expressing green fluorescent prot

275 Type I glial-like taste cells, but not other taste cell types.

276 calizes with markers of type II and type III taste cells: ubiquitin carboxyl terminal hydrolase (PGP

277 Taste cells undergo constant turnover throughout life; h

278 To investigate whether taste cells undergo regulated exocytosis, we used the co

279 Taste cells use multiple signalling mechanisms to genera

280 1 as the acid-sensitive K(+) channel in sour taste cells using pharmacological and RNA expression pro

281 stinct functions for PYY produced locally in taste cells vs. that circulating systemically.

282 the total outward current of mouse fungiform taste cells was composed of K(ATP) channels.

283 In contrast, the number of differentiated taste cells was not significantly reduced until 7 dpi.

284 ing Fura-2 imaging of isolated mouse vallate taste cells, we explored how elevating cAMP alters Ca(2+

285 on studies on isolated taste buds and single taste cells, we have postulated that ATP secreted from r

286 Using calcium imaging of isolated mouse taste cells, we identify two separate populations of AI

287 e transcriptase (RT)-PCR on isolated vallate taste cells, we show that many Receptor cells express th

288 differentiate into different types of mature taste cells, we sought to identify genes that were selec

289 clusively by three pairs of bitter-sensitive taste cell, which are located in the medial, lateral, an

290 Taste buds are comprised of taste cells, which are classified into types I to IV.

291 -10 is produced by only a specific subset of taste cells, which are different from the TNF-producing

292 ation generates excitatory responses in sour taste cells, which can be attributed to block of a resti

293 TRPM5 is believed to depolarize taste cells, which leads to the release of ATP, which ac

294 logic responses to umami stimuli in isolated taste cells, which suggests that cAMP may have a modulat

295 ld elicit depolarization of sweet-responsive taste cells, which would transmit their signal to gustat

296 pends on continuous replacement of senescent taste cells with new ones generated by adult taste stem

297 examined was heterogeneously distributed in taste cells with notably more GABA positive cells presen

298 Previous studies have shown that taste cells with synapses display SNAP-25- and synaptobr

299 at immunoreactivity to SNAP-25 is present in taste cells with synapses.

300 express synaptobrevin-2-LIR, as well as some taste cells without synapses.