ライフサイエンスコーパス: taurocholate

1 etrograde pancreatic duct infusion of sodium taurocholate).

2 urolithocholate), and hepatocyte ballooning (taurocholate).

3 nt mice were protected from these effects of taurocholate.

4 r network formation similar to the effect of taurocholate.

5 res with greater apparent affinity than does taurocholate.

6 ure to glycine and certain bile acids, e.g., taurocholate.

7 difficile spores in response to cholate and taurocholate.

8 id palmitate, and the bile acids cholate and taurocholate.

9 e and the apical-to-basolateral transport of taurocholate.

10 nalicular membrane by cAMP and the other, by taurocholate.

11 onfers functional transport of the bile salt taurocholate.

12 est natural substrates being isethionate and taurocholate.

13 vered from explants after exposure to sodium taurocholate.

14 Na+-dependent transcellular transport of [3H]taurocholate.

15 nin was administered prior to treatment with taurocholate.

16 saturable, exhibiting a Km of 63 microM for taurocholate.

17 tides to MTP was enhanced in the presence of taurocholate.

18 induced TcpP dimerization in the presence of taurocholate.

19 red with their redox state in the absence of taurocholate.

20 of cerulein or pancreatic infusion of sodium taurocholate.

21 an abiotic degradation of biofilm matrix by taurocholate.

22 rowth factor, interleukin-6, and then sodium taurocholate.

23 n or by intraductal administration of sodium taurocholate.

24 ne of these was revealed to be the bile salt taurocholate.

25 rats by intraductal infusion of 3.5% sodium taurocholate.

26 The intraduodenal infusion of taurocholate (24 hours) in biliary-diverted rats resulte

27 g(-1) x min(-1)) on biliary recovery of (3)H-taurocholate ((3)H-TC) and (3)H-cholate administered int

28 [(3)H]Taurocholate ([(3)H]TC) uptake decreased in WIF-B cells

29 Intraduodenal infusion of taurocholate (36 micromol/h. 100 g rat(-1)) decreased SA

30 cile Ger receptors have not been identified, taurocholate (a bile salt) and glycine (an amino acid) h

31 Taurocholate (a bile salt) and glycine (an amino acid) h

32 the other hand, C. difficile spores require taurocholate (a bile salt) and glycine (an amino acid) t

33 films to physiologic levels of the bile salt taurocholate, a host signal for the virulence gene induc

34 porters from intrahepatic sites with cAMP or taurocholate, a significant increase in the amount of AB

35 Taurocholate accelerated canalicular network formation a

36 re germination kinetics to determine whether taurocholate acts as a specific germinant that activates

37 us BS (50%-80%, P < 0.05) as well as that of taurocholate administered in choleretic or trace radiola

38 Perfusion of liver with wortmannin before taurocholate administration blocked accumulation of all

39 ey further investigated the mechanism of how taurocholate affects V. cholerae virulence determinants.

40 only able to induce virulence in response to taurocholate after exit from the biofilm.

41 lesterol, 22.9% egg yolk lecithin, and 68.6% taurocholate (all mole %) were vitrified at 2 min to 20

42 -dependent induction, some bile salts (e.g., taurocholate) also activated the expression of cmeABC by

43 Uptake of [3H]taurocholate, an endogenous substrate of oatp1, was comp

44 Finally, a taurocholate analog with an m-aminobenzenesulfonic acid

45 ent study, we tested a series of glycine and taurocholate analogs for the ability to induce or inhibi

46 Testing of taurocholate analogs revealed that the 12-hydroxyl group

47 arly, C. difficile spores are able to detect taurocholate analogs with shorter, but not longer, alkyl

48 marked increases in tauro-beta-muricholate, taurocholate and 12-hydroxyeicosatetraenoic acid (12-HET

49 trafficking in rat liver induced by cAMP or taurocholate and [(35)S]methionine metabolic labeling fo

50 ectrokinetic chromatography system of sodium taurocholate and chromatographic measurements using an i

51 ase also regulate ATP-dependent transport of taurocholate and dinitrophenyl-glutathione directly in c

52 ATP-dependent transport of taurocholate and dinitrophenyl-glutathione in isolated c

53 n canalicular membrane vesicles and enhanced taurocholate and dinitrophenyl-glutathione transport in

54 ested the presence of distinct receptors for taurocholate and glycine.

55 levels of taurine, lathosterol, bile acids (taurocholate and glycodeoxycholate), nicotinamide, and a

56 constant intraduodenal infusion of micellar taurocholate and lecithin.

57 Of significance, oatp1-mediated taurocholate and LTC4 uptake was cis-inhibited and trans

58 nd cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme (CCMB-TAL) were compared for r

59 o the plasma membrane; however, transport of taurocholate and other bile acids was abolished.

60 ATP-dependent transport of taurocholate and PI 3-kinase activity were reduced in ca

61 ease in sensitivity to the bile salts sodium taurocholate and sodium deoxycholate and significantly i

62 greater for the more hydrophilic bile salts taurocholate and tauroursodeoxycholate, for EYPC/Ch vesi

63 SPGP cells had decreased uptake of taurocholate and vinblastine compared with LLC-PK1 cells

64 mic response was blocked on co-infusion with taurocholate and was absent for infusion of the conjugat

65 d from rat liver that had been perfused with taurocholate and wortmannin.

66 -arginine, the retrograde infusion of sodium taurocholate, and another novel model with concomitant a

67 er progenitor cells (LPCs) were treated with taurocholate, and key events in DR evolution were assess

68 the monoanionic bile acids glycocholate and taurocholate, and methotrexate.

69 clodextrin as the chiral selector and sodium taurocholate as the micelle-forming agent.

70 more extensively reduced in the presence of taurocholate, as compared with their redox state in the

71 with conjugated bile salts (glycocholate and taurocholate) being less active than unconjugated bile s

72 Taurocholate bound to VcDsbA with a KD of 40 +/- 2.5 muM

73 ctose agar (CCFA), CCFA with horse blood and taurocholate (CCFA-HT), and cycloserine-cefoxitin mannit

74 whether it is the recently described sodium-taurocholate co-transporter polypeptide (NTCP), encoded

75 imulates taurocholate (TC) uptake and sodium taurocholate co-transporting polypeptide (Ntcp) transloc

76 ncipal hepatic bile acid importer, the Na(+)/taurocholate co-transporting polypeptide (Ntcp, Slc10a1)

77 ed in enterohepatic recirculation, the Na(+)-taurocholate co-transporting polypeptide (NTCP; also kno

78 secretion by studying the in vivo effect of taurocholate, colchicine, and wortmannin on bile acid se

79 able cell and heat-resistant spore counts on taurocholate-containing media.

80 The Na+-taurocholate cotransport polypeptide (ntcp) is the prima

81 The BA transporter high-affinity Na+ /taurocholate cotransporter (NTCP) and the BA synthesizin

82 l functions of the cytoplasmic tail of Na(+)/taurocholate cotransporter (Ntcp) and to determine the b

83 Expression and function of the hepatic Na+/taurocholate cotransporter (ntcp) are down-regulated in

84 the circulation by the liver-specific sodium/taurocholate cotransporter (SLC10A1).

85 In contrast, expressions of the Na(+) taurocholate cotransporter and multispecific organic ani

86 or basolateral bile acid transporters sodium taurocholate cotransporter protein and organic anion tra

87 ther hepatocyte membrane transporters (Na(+) taurocholate cotransporter, multispecific organic anion

88 nd required TUDC uptake by way of the Na(+) /taurocholate cotransporting peptide.

89 rus and hepatitis delta virus use the sodium/taurocholate cotransporting polypeptide (a bile acid tra

90 sion of the HBV entry receptor, human sodium-taurocholate cotransporting polypeptide (hNTCP), on maca

91 host factors like the receptor human sodium taurocholate cotransporting polypeptide (hNTCP).

92 in HuH7 cells stably transfected with sodium taurocholate cotransporting polypeptide (HuH-NTCP cells)

93 polypeptide (OATP) 1B1 and sodium-dependent taurocholate cotransporting polypeptide (NTCP) allelic v

94 interact with the HBV entry receptor sodium taurocholate cotransporting polypeptide (NTCP) and impai

95 ation of plasma membrane localization of Na+-taurocholate cotransporting polypeptide (NTCP) and multi

96 Identification of sodium taurocholate cotransporting polypeptide (NTCP) as an HBV

97 The discovery of sodium taurocholate cotransporting polypeptide (NTCP) as the he

98 derlying the upregulation of the hepatic Na+/taurocholate cotransporting polypeptide (ntcp) by prolac

99 Sodium taurocholate cotransporting polypeptide (Ntcp) from the

100 Although sodium taurocholate cotransporting polypeptide (NTCP) has recen

101 tracellular Na(+) and the presence of sodium-taurocholate cotransporting polypeptide (NTCP) indicate

102 The sodium taurocholate cotransporting polypeptide (Ntcp) is the ma

103 The Na(+) -taurocholate cotransporting polypeptide (NTCP) mediates

104 ssion, protein mass, and function of the Na+/taurocholate cotransporting polypeptide (Ntcp), common b

105 ctional characterization of the shrew sodium taurocholate cotransporting polypeptide (Ntcp), CSHBV/MS

106 porters (bile salt export pump (BSEP), Na(+)/taurocholate cotransporting polypeptide (NTCP), OATP1, O

107 Moreover, the Na+-taurocholate cotransporting polypeptide (NTCP), responsi

108 ity in HepG2 cells reconstituted with sodium taurocholate cotransporting polypeptide (NTCP), the curr

109 larity on short term regulation of the Na(+)-taurocholate cotransporting polypeptide (Ntcp), the majo

110 SLC10A1 codes for the sodium-taurocholate cotransporting polypeptide (NTCP), which is

111 V infection of HepG2 cells expressing sodium taurocholate cotransporting polypeptide (NTCP).

112 e L protein subsequently binds to the sodium taurocholate cotransporting polypeptide (NTCP, encoded b

113 of bile acids from portal circulation is Na+-taurocholate cotransporting polypeptide (NTCP, SLC10A1).

114 The Na(+) -taurocholate cotransporting polypeptide (NTCP/SLC10A1) i

115 (HBVpreS) to specifically target the sodium-taurocholate cotransporting polypeptide (NTCP; SLC10A1)

116 curred through the human HBV receptor sodium taurocholate cotransporting polypeptide but could not be

117 The Na(+)-taurocholate cotransporting polypeptide SLC10A1 (NTCP) p

118 However, common BA importers (Na(+)/taurocholate cotransporting polypeptide, apical sodium-d

119 lls were stably transfected with human Na(+)-taurocholate cotransporting polypeptide, BSEP, MDR3, and

120 peptide (Oatp) 1a1, Oatp1a4, Oatp1b2, sodium taurocholate cotransporting polypeptide, multidrug resis

121 le transporters (e.g., ABCB4, ABCB11, sodium/taurocholate cotransporting polypeptide, organic solute

122 he liver basolateral membrane protein, Na(+)/taurocholate cotransporting polypeptide, with the 14-mer

123 m-dependent bile acid transporter and Na(+) -taurocholate cotransporting polypeptide, within the ente

124 he farsenoid X receptor and sodium-dependent taurocholate cotransporting polypeptide; new insights in

125 ic bile salt uptake, by inhibition of sodium-taurocholate cotransporting polypetide (NTCP; Slc10a1) w

126 conjugated bile acids, mediated by the Na(+)-taurocholate cotransporting protein (NTCP).

127 ted alterations of the hepatocellular sodium-taurocholate-cotransporting polypeptide (ntcp).

128 primers for both the rat liver Na+-dependent taurocholate-cotransporting polypeptide and rat ileal ap

129 orters OATP1B2, OATP1A1, OATP1A4, and Na(+) -taurocholate-cotransporting polypeptide only in central

130 We determined the increment in taurocholate-dependent bile flow and biliary lipid secre

131 to HCO3- extrusion, we compared the rates of taurocholate-dependent HCO3- efflux from alkali-loaded n

132 rt the model bile acid transporter substrate taurocholate (despite the pronounced sensitivity of both

133 Tbeta also were able to mediate transport of taurocholate, digoxin, and prostaglandin E2 but not of e

134 Conjugated cholate and taurocholate directly and secondary to primary BA ratio

135 In mice given taurocholate, DNase I administration also reduced expres

136 -dependent PKA inhibitor did not prevent the taurocholate effect.

137 ownstream targets, Rap1 and MEK, blocked the taurocholate effect.

138 Here we show that cholate and taurocholate elicit more dramatic Ca(2+) signals and nec

139 ulfophthalein or probenecid was observed for taurocholate, estrone sulfate, and para-aminohippurate i

140 ut not each separately, were able to take up taurocholate, estrone sulfate, digoxin, and prostaglandi

141 The stoichiometry of GSH/taurocholate exchange was 1:1.

142 Cell-free media from taurocholate-exposed biofilms contains a larger amount o

143 antibiotics, including the primary bile acid taurocholate for germination, and carbon sources such as

144 Furthermore, taurocholate, glycine, and chenodeoxycholate seem to bin

145 occurring bile salts: cholate, glycocholate, taurocholate, glycochenodeoxycholate, and taurochenodeox

146 of bilirubin, sulfobromophthalein (BSP), and taurocholate, had any influence on 55Fe-heme uptake.

147 ptone no. 2, and the concentration of sodium taurocholate has been reduced from 0.1% to 0.05%.

148 sis in animal models, whereas the bile acid, taurocholate, has protective effects in animal models of

149 ganic anion uptake, we examined transport of taurocholate in a HeLa cell line stably transfected with

150 Everted membrane vesicles accumulated taurocholate in an energy-dependent manner, apparently c

151 This study describes a unique function of taurocholate in bile canalicular formation involving sig

152 hus, we performed transport studies with [3H]taurocholate in confluent polarized monolayers of normal

153 hibits uptake and vasoconstrictor effects of taurocholate in human placenta.

154 As with Oatp1, uptake of 10 microM [(3)H]taurocholate in Oatp2-expressing Xenopus laevis oocytes

155 ecules are significantly more effective than taurocholate in promoting the intestinal absorption of a

156 Sodium-dependent uptake of (3)H-taurocholate in renal brush border membrane vesicles was

157 presence of the bile salts glycocholate and taurocholate in the small intestine causes dimerization

158 hosphate enhanced ATP-dependent transport of taurocholate in these vesicles above control levels.

159 of cholangiocytes, we examined the effect of taurocholate (in the absence or presence of wortmannin o

160 Taurocholate increased protein content of ATP-dependent

161 Infusion of taurocholate increased the transcriptional activity of N

162 y acid (propionate) and major rat bile acid (taurocholate) indicating a fundamental position for GLUT

163 stimulated by intracellular 0.2 mM unlabeled taurocholate, indicating bidirectional transport.

164 ng of LDL was enhanced by preincubation with taurocholate, indicating that partial delipidation of ap

165 additional administration of cAMP but not by taurocholate, indicating two distinct intrahepatic pools

166 Taurocholate induced a time-dependent increase in LPC pr

167 Sodium cholate and taurocholate induced cytosolic Ca(2+) elevations in stel

168 Infusion of taurocholate induced formation of NETs in pancreatic tis

169 Taurocholate induced LPCs to release MCP-1, MIP1alpha, a

170 Although taurocholate induced similar changes in circular dichroi

171 Inhibition of NFAT with A-285222 blocked taurocholate-induced activation of NFAT in all organs.

172 groups of rats with either mild or moderate taurocholate-induced AP and sham controls.

173 Wortmannin blocked taurocholate-induced bile acid secretion.

174 deoxycholate, another bile salt, can inhibit taurocholate-induced C. difficile spore germination.

175 Colchicine prevented taurocholate-induced changes in all proteins studied, as

176 Neutrophil depletion prevented taurocholate-induced deposition of NETs in the pancreas.

177 A-285222 also reduced taurocholate-induced increases in levels of amylase, mye

178 ve compounds were extracted and screened for taurocholate-induced necrosis in mouse pancreatic acinar

179 oinflammatory genes in vivo in the course of taurocholate-induced necrotizing pancreatitis in rats an

180 derivatives have been prepared that inhibit taurocholate-induced spore germination.

181 feres with bile acid secretion in rat liver; taurocholate induces recruitment of ATP-dependent transp

182 A taurocholate influx assay and membrane biotinylation ana

183 , or sodium taurocholate (the bile-depleted, taurocholate-infused group).

184 In bile-depleted, taurocholate-infused rats, cholangiocyte proliferation a

185 In addition, the bile acid taurocholate inhibited the uptake of 3H-MPP+ in oocytes

186 Taurocholate inhibited VcDsbA reductase activity without

187 Infusion of Na-taurocholate into the pancreatic duct induced necrotizin

188 P was induced in C57BL/6 mice by infusion of taurocholate into the pancreatic duct or by intraperiton

189 er induction of AP by retrograde infusion of taurocholate into the pancreatic ducts of wild-type, NFA

190 C transporter trafficking induced by cAMP or taurocholate is a physiologic response to a temporal dem

191 This study suggests that taurocholate is involved in initiating functional LPC bi

192 alogs revealed that the 12-hydroxyl group of taurocholate is necessary, but not sufficient, to activa

193 In the absence of extracellular Cl- and taurocholate, isohydric reduction of superfusate HCO3- c

194 iency leads to impaired biliary excretion of taurocholate, lecithin, and water, while cholesterol tra

195 sulin and glycaemic pathways, and related to taurocholate levels in blood.

196 ated with hepatic fibrosis stage and biliary taurocholate levels.

197 associated with higher grades of steatosis (taurocholate), lobular (glycocholate) and portal inflamm

198 Efflux of [3H]taurocholate mediated by CBATP in the cotransfected COS

199 These results indicate that taurocholate-mediated changes involve a microtubular sys

200 nodeoxycholate is a competitive inhibitor of taurocholate-mediated germination and appears to interac

201 enodeoxycholate, another bile acid, inhibits taurocholate-mediated germination.

202 yte containing gamma-cyclodextrin and sodium taurocholate micelles.

203 e-sensitive uptake of [(3)H]cholesterol from taurocholate micelles.

204 [(3)H]sn-2-monoolein was examined by adding taurocholate-mixed sn-2-18:1 and albumin-bound sn-2-18:1

205 e sn-2-18:1 was incorporated into TG from AP taurocholate-mixed sn-2-18:1, whereas more phospholipid

206 The position of the taurocholate molecule, together with the molecular archi

207 ependent process that tightly companied with taurocholate movement.

208 port measured and its inability to transport taurocholate, MRP3, like MRP1 and cMOAT, is concluded to

209 ered sequential progression of binding where taurocholate must be recognized first before detection o

210 Na+/taurocholate (Na+/TC) cotransport in hepatocytes is medi

211 Bile salts such as sodium taurocholate (NaTC) are routinely used to induce the exc

212 ar GSH failed to cis-inhibit uptake of [(3)H]taurocholate or [(3)H]digoxin in Oatp2-expressing oocyte

213 rats by either retrograde infusion of sodium taurocholate or by direct trauma.

214 hours, followed by intraduodenal infusion of taurocholate or fluid/electrolytes.

215 strain but only marginally more sensitive to taurocholate or glycocholate.

216 as enhanced in the presence of extracellular taurocholate or sulfobromophthalein, indicating that GSH

217 Infusion of either taurocholate or taurochenodeoxycholate for 12 hours also

218 ancreatic AR42J acinar cells stimulated with taurocholate or TNF-alpha.

219 fected by brefeldin A, dibutyryl cyclic AMP, taurocholate, or PI 3-kinase inhibitors.

220 ispersed in mixed micelles containing sodium taurocholate, phosphatidylcholine, and cholesterol remai

221 ling endosome, whereas recruitment from the "taurocholate-pool" involves a hepatocyte-specific mechan

222 M) structure was captured with the substrate taurocholate present, bound between the core and panel d

223 Taurocholate rapidly activated MEK, LKB1, and AMPK, whic

224 icroscopy micrographs of biofilms exposed to taurocholate revealed an altered, perhaps degraded, appe

225 en shown that a set of bile salts, including taurocholate, serve as host signals to activate V. chole

226 of rats or perfusion of isolated liver with taurocholate significantly increased PI 3-kinase activit

227 Sodium taurocholate (STC) belongs to a major class of bile salt

228 The inhibitory effect of wortmannin on taurocholate stimulation of cholangiocyte proliferation

229 In vitro, taurocholate stimulation of DNA replication and secretin

230 ncrease in bile flow and bile salt output in taurocholate-supplemented isolated perfused livers but h

231 ple and mixed micelles prepared using sodium taurocholate (TA) alone or with egg lecithin (LE) were t

232 cted cells showed virtually no uptake of [3H]taurocholate, taurocholate uptake by induced cells was N

233 Taurocholate (TC) and dibutyryl cAMP (DBcAMP), stimulato

234 Hepatic uptake of intravenously infused taurocholate (TC) and taurochenodeoxycholate (TCDC) were

235 Other natural bile acids, taurocholate (TC) and taurodeoxycholate (TDC), were also

236 By this mechanism, taurocholate (TC) and taurolithocholate (TLC) increase c

237 We have shown that taurocholate (TC) and taurolithocholate (TLC) interact i

238 nt bile flow and biliary lipid secretion and taurocholate (TC) biliary transit time during high ASBT

239 res the placental transport of the bile acid taurocholate (TC) by the organic anion-transporting poly

240 yclic AMP has been proposed to stimulate Na+/taurocholate (TC) cotransport in hepatocytes by transloc

241 P-mediated translocation of sinusoidal Na(+)/taurocholate (TC) cotransporter (Ntcp) to the plasma mem

242 ate (cAMP) stimulates translocation of Na(+)-taurocholate (TC) cotransporting polypeptide (Ntcp) and

243 tic bile acid uptake by translocating sodium-taurocholate (TC) cotransporting polypeptide (Ntcp) from

244 activity of the bile salt transporters Na(+)/taurocholate (TC) cotransporting polypeptide (Ntcp), and

245 d transport (ASBT)-mediated uptake of [(14)C]taurocholate (TC) in H14 cells.

246 by examining sodium-dependent uptake of [3H]-taurocholate (TC) into brush border membrane vesicles (B

247 We examined the effect of taurocholate (TC) on the basolateral uptake of [3H]TC in

248 Support livers were provoked with taurocholate (TC) to enhance bile aqueous and hydrophobi

249 ed by Northern blotting, immunoblotting, and taurocholate (TC) transport studies in isolated short-te

250 cell swelling- and cAMP-induced increases in taurocholate (TC) uptake and Ntcp translocation in hepat

251 Cyclic AMP stimulates taurocholate (TC) uptake and sodium taurocholate co-tran

252 bile acid clearance increased from 15% when taurocholate (TC) was infused alone to 46% (P=0.007) whe

253 ells, taurochenodeoxycholate (TCDC), but not taurocholate (TC), induced endocytosis of Ntcp.

254 d the effects of three different bile salts, taurocholate (TC), tauroursodeoxycholate (TUDC), and tau

255 ic acid (OA) together with either albumin or taurocholate (TC).

256 ne triphosphate (ATP)-dependent transport of taurocholate (TC, 1 micromol/L) in membrane vesicles was

257 er RNA (mRNA) levels in the following order: taurocholate (TCA) (288 +/- 36%, P <.005) = taurodeoxych

258 tion in the integral membrane protein, Na(+)/taurocholate (TCA) cotransporting polypeptide, at the si

259 In primary rat hepatocyte cultures, taurocholate (TCA) strongly activated JNK in a time- and

260 e receptor 2 (S1P(2) ) as being activated by taurocholate (TCA).

261 P after administration of cerulein or sodium taurocholate than control mice; pancreata from the Bcl3(

262 n Vibrio cholerae by degrading the bile salt taurocholate that activates the expression of virulence

263 nseleit (the bile-depleted group), or sodium taurocholate (the bile-depleted, taurocholate-infused gr

264 When wortmannin was given after taurocholate, the protein levels of each ATP-dependent t

265 en insulin was co-infused with the bile salt taurocholate, this was followed by a marked hypoglycaemi

266 ficile spores recognize both amino acids and taurocholate through multiple interactions that are requ

267 s show that when IBAT mediates uptake of [3H]taurocholate to a level 20-fold higher than that achieve

268 Administration of cAMP or taurocholate to rats increased amounts of SPGP, MDR1, an

269 Addition of 25 microM taurocholate to the superfusate led to a rapid fall in p

270 little skate Raja erinacea, was screened for taurocholate transport activity by using Xenopus laevis

271 of the C terminus of Ostbeta abolished [(3)H]taurocholate transport activity, but reinsertion of two

272 7G, G982R, R1153C, and R1268Q also abolished taurocholate transport activity, possibly by causing Bse

273 plasma membrane and generate cellular [(3)H]taurocholate transport activity.

274 Taurocholate transport by two assessed mutants, N490D an

275 assessed by adenosine triphosphate-dependent taurocholate transport in canalicular membrane vesicles,

276 These data indicate that oatp-mediated taurocholate transport is Na+-independent, saturable, an

277 were identified as functionally important by taurocholate transport studies, substrate inhibition ass

278 talpha(-/-) vs. wild-type mice; the residual taurocholate transport was further reduced to near-backg

279 Apical phospholipid secretion and taurocholate transport were assayed to investigate the e

280 ted from HTC-R cells exhibited ATP-dependent taurocholate transport, which was many-fold greater than

281 ate-stimulated ATPase activity as well as on taurocholate transport.

282 r plasma membrane fractions from control and taurocholate-treated rats correlated positively with ade

283 e mutated Ntcps followed by determination of taurocholate uptake and Ntcp expression, translocation a

284 ylation and in 2.5 and 3.2-fold increases in taurocholate uptake and Ntcp retention in the plasma mem

285 wed virtually no uptake of [3H]taurocholate, taurocholate uptake by induced cells was Na+-independent

286 Interestingly, [(3)H]taurocholate uptake in Oatp2-expressing oocytes was also

287 ere assessed by a combination of functional (taurocholate uptake into crude brush border membrane ves

288 Similarly, sodium-dependent [3H]taurocholate uptake into membrane vesicles from cholic a

289 Cells were analyzed for Myrcludex B binding, taurocholate uptake, HBV covalently closed circular DNA

290 ll alanine insertions in H3 and H8 abolished taurocholate uptake, suggesting that both these regions

291 e mutants displayed active, sodium-dependent taurocholate uptake.

292 ontrast, the A171S mutation had no effect on taurocholate uptake.

293 Taurocholate, used to solubilize the fatty acids, did no

294 transfected with IBAT and CEA, efflux of [3H]taurocholate was detected in COS cells cotransfected wit

295 lein, and 2.6 mm [(14)C]cholesterol in 19 mm taurocholate was infused into the duodenum of lymph fist

296 A Na+-dependent saturable uptake of taurocholate was present in large but not small cholangi

297 gut sac experiments, transileal transport of taurocholate was reduced by >80% in Ostalpha(-/-) vs. wi

298 lower than in WT mice, but the clearance of taurocholate was similar in the two genotypes.

299 Water-soluble vitamins B2, B5, B6, and taurocholate were also detected with high fold change in

300 closed saturable Na+-dependent uptake of [3H]taurocholate, with apparent Km and Vmax values of 209+/-