ライフサイエンスコーパス: tautomerase

1 rly zebrafish melanoblast marker, dopachrome tautomerase.

2 gulatory protein as well as a phenylpyruvate tautomerase.

3 an inhibitor of the enzyme 4-oxalocrotonate tautomerase.

4 DH) and Rpa1177, a putative 4-oxalocrotonate tautomerase.

5 h two bacterial isomerases, 4-oxalocrotonate tautomerase (4-OT) and 5-(carboxymethyl)-2-hydroxymucona

6 us to the bacterial enzymes 4-oxalocrotonate tautomerase (4-OT) and 5-carboxymethyl-2-hydroxymuconate

7 4-Oxalocrotonate tautomerase (4-OT) and trans-3-chloroacrylic acid dehalo

8 nhibitors of three enzymes: 4-oxalocrotonate tautomerase (4-OT) and vinylpyruvate hydratase (VPH) fro

9 4-Oxalocrotonate tautomerase (4-OT) and YwhB, a 4-OT homologue found in B

10 e reaction catalyzed by the 4-oxalocrotonate tautomerase (4-OT) enzyme has been studied using a quant

11 62 amino acid member of the 4-oxalocrotonate tautomerase (4-OT) family, was pro-immunogenic in mice w

12 d with other members of the 4-oxalocrotonate tautomerase (4-OT) family.

13 e amino-terminal proline of 4-oxalocrotonate tautomerase (4-OT) functions as the general base catalys

14 lyst Pro-1 (pK(a) = 6.4) in 4-oxalocrotonate tautomerase (4-OT) has been ascribed to both a low diele

15 r the kinetic parameters of 4-oxalocrotonate tautomerase (4-OT) have been measured using 2-hydroxy-2,

16 YwhB, a 4-oxalocrotonate tautomerase (4-OT) homologue in Bacillus subtilis, has n

17 The crystal structure of 4-oxalocrotonate tautomerase (4-OT) inactivated by the active site-direct

18 4-Oxalocrotonate tautomerase (4-OT) is a bacterial enzyme that is compris

19 4-Oxalocrotonate tautomerase (4-OT) is a multimeric, bacterial enzyme com

20 4-Oxalocrotonate tautomerase (4-OT) isozymes play prominent roles in the

21 jor families represented by 4-oxalocrotonate tautomerase (4-OT), 5-(carboxymethyl)-2-hydroxymuconate

22 tion secondary structure of 4-oxalocrotonate tautomerase (4-OT), a 41 kDa homohexamer with 62 residue

23 4-Oxalocrotonate tautomerase (4-OT), a homohexameric enzyme, converts the

24 lecular weight 47,547), and 4-oxalocrotonate tautomerase (4-OT), an (alpha)(6) homohexamer, distingui

25 nd the bacterial isomerase, 4-oxalocrotonate tautomerase (4-OT), thereby distinguishing CaaD from a n

26 cluding carbonic anhydrase, 4-oxalocrotonate tautomerase (4OT) analogue, and SecB, a chaperone from E

27 de backbone interactions in 4-oxalocrotonate tautomerase (4OT) catalysis has been investigated using

28 of the homohexameric enzyme 4-oxalocrotonate tautomerase (4OT).

29 , Tyr, Tyr-related protein 1, and dopachrome tautomerase accumulated in enlarged granules distributed

30 n fluorophore with high affinity for the MIF tautomerase active site (K(i) = 18 +/- 1 nM) that binds

31 Selected compounds that bind in the tautomerase active site also inhibit biological function

32 ytometry, concluding a critical role for the tautomerase active site in receptor binding.

33 Molecules binding to the MIF tautomerase active site interfere with its biological ac

34 ian MIFs, it has critical differences in the tautomerase active site that account for the different i

35 y docking 2.1 million compounds into the MIF tautomerase active site.

36 l-molecular-weight inhibitor targeting MIF's tautomerase activity (SCD-19) significantly reduces the

37 In addition, it exhibits a catalytic tautomerase activity amenable to the design of high affi

38 ibitor 5d with an IC(50) of 1.0 muM for MIF2 tautomerase activity and a high selectivity over MIF.

39 Like MIF-1, MIF-2 has intrinsic keto-enol tautomerase activity and mediates biological functions b

40 een described recently to exhibit dopachrome tautomerase activity and to be structurally homologous t

41 However, MIF tautomerase activity assays are troubled by irregulariti

42 For instance, the IC(50) inhibition of MIF tautomerase activity by aromatic amino acid Schiff base

43 To investigate the role of MIF's tautomerase activity in a murine model of Pseudomonas ae

44 sted a potential explanation for the lack of tautomerase activity in the MDLs.

45 ine oxidation drop rapidly, while DOPAchrome tautomerase activity increases and dihydroxyindole carbo

46 MIF-tautomerase activity may provide a novel therapeutic tar

47 ted for biaryltriazoles as inhibitors of the tautomerase activity of human macrophage migration inhib

48 ibitors of the p-hydroxyphenylpyruvate (HPP) tautomerase activity of MIF and an allosteric binding si

49 Epoxyazadiradione inhibited the tautomerase activity of MIF of both human (huMIF) and ma

50 pyruvate, a substrate for the phenylpyruvate tautomerase activity of MIF.

51 ame as the catalytic site for the dopachrome tautomerase activity of MIF.

52 To identify small-molecule inhibitors of the tautomerase activity of PfMIF, virtual screening has bee

53 th human MIF, yet only have minimal residual tautomerase activity using either p-hydroxyphenylpyruvat

54 MIF inhibitors has focused on monitoring the tautomerase activity using l-dopachrome methyl ester or

55 The most potent inhibitor of MIF tautomerase activity was 2-[(4-hydroxybenzylidene)amino]

56 Inhibition of MIF tautomerase activity was also established for many of th

57 t lacks oxidoreductase activity but exhibits tautomerase activity with a specific activity of 19.3 mu

58 rongly correlated with the inhibition of MIF tautomerase activity, a connection not made previously t

59 oxycinnamate, a competitive inhibitor of the tautomerase activity, has been determined to 1.8 A resol

60 Here, we show that TvMIF has tautomerase activity, inhibits macrophage migration, and

61 The effect of a specific inhibitor of MIF-tautomerase activity, ISO-1, was investigated in PBMCs.

62 teins were compared using in vitro assays of tautomerase activity, macrophage migration, and binding

63 er (ISO-1), an inhibitor of MIF d-dopachrome tautomerase activity, reveals that ISO-1 binds to the sa

64 As for other MIF orthologues, PfMIF has tautomerase activity, whose inhibition may influence the

65 elatively nonspecific 1,3- and 1,5-keto-enol tautomerase activity, with the former activity prevailin

66 e significantly reduced or no phenylpyruvate tautomerase activity.

67 city to interact with this cytokine's unique tautomerase activity.

68 discovered by testing against its keto/enol tautomerase activity.

69 lines, which show in vitro inhibition of MIF tautomerase activity.

70 a covalent complex with MIF and inhibits the tautomerase activity.

71 macrophage migration inhibitory factor (MIF) tautomerase activity.

72 pro-inflammatory activities by targeting MIF tautomerase activity.

73 We designed small molecules to inhibit this tautomerase activity; a lead molecule, "ISO-1 ((S,R)-3-(

74 elanogenic enzymes tyrosinase and dopachrome tautomerase, all major players in melanogenesis.

75 nal similarity, the MIF homolog D-dopachrome tautomerase (also called MIF-2) has low sequence identit

76 tein family consists of MIF and D-dopachrome tautomerase (also known as MIF-2).

77 hancer required for expression of dopachrome tautomerase, an enzyme that functions in melanin synthes

78 ve site similar to those of 4-oxalocrotonate tautomerase and 5-carboxymethyl-2-hydroxymuconate isomer

79 t of two microbial enzymes (4-oxalocrotonate tautomerase and 5-carboxymethyl-2-hydroxymuconate isomer

80 inhibitory factor (MIF) is both a keto-enol tautomerase and a cytokine associated with numerous infl

81 of benzaldehyde derivatives that inhibit MIF tautomerase and biological activities.

82 d mechanism of action, the protein is also a tautomerase and has a catalytically active N-terminal pr

83 and tyrosinase-related protein 2/dopachrome tautomerase and increased protection from a lethal chall

84 als that, unusually for a cytokine, exhibits tautomerase and oxidoreductase enzymatic activities.

85 pment and pigmentation, including dopachrome tautomerase and tyrosinase.

86 se, tyrosinase-related protein-1, dopachrome tautomerase, and Pmel17, are known, the function of MART

87 -associated transcription factor, dopachrome tautomerase, and tyrosinase promoters, leading to an inc

88 y factor (MIF) and its paralog, D-dopachrome tautomerase, are multifunctional inflammatory cytokines.

89 ion inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT or MIF2) play key roles in cancers.

90 anding of the MIF homolog MIF-2/d-dopachrome tautomerase (d-DT) and its clinical translation has been

91 tein encoded by the homologous, D-dopachrome tautomerase (D-DT) gene.

92 onally redundant family member, D-dopachrome tautomerase (D-DT), also remains to be further character

93 itory factor (MIF), its homolog D-dopachrome tautomerase (D-DT), and their common receptor CD74 may c

94 IF) and its functional homolog, d-dopachrome tautomerase (d-DT), have protumorigenic functions in non

95 d its only known family member, D-dopachrome tautomerase (D-DT), promote the expression of proangioge

96 or the immunomodulatory protein D-dopachrome tautomerase (D-DT), the mechanism of protein-ligand inte

97 tory activity for extracellular d-dopachrome tautomerase (D-DT), the recruitment of neutrophils to th

98 Plant MIF/D-dopachrome tautomerase (D-DT)-like proteins (MDLs) can interact wit

99 pyruvate dioxygenase (HPPD) and D-dopachrome tautomerase (D-DT); two additional enzymes that utilize

100 second MIF superfamily member, D-dopachrome tautomerase (D-DT/MIF-2), prompted closer investigation

101 TRP1) and 3,4-dihydroxyphenylalanine-chrome tautomerase (Dct or TRP2) encoded at the Tyrp1/brown and

102 Tyrosinase and dopachrome tautomerase (DCT) activities were found exclusively in s

103 DOPAchrome tautomerase (Dct) functions downstream of tyrosinase in

104 The melanin synthesis enzyme dopachrome tautomerase (DCT) is involved in intracellular calcium a

105 tyrosinase minigene driven by the dopachrome tautomerase (Dct) promoter region.

106 pment in transgenic mice from the dopachrome tautomerase (Dct) promoter.

107 sinase-related protein-1 (Tyrp1), dopachrome tautomerase (Dct), and LAMP1 and 3 localization in HPS-3

108 al precursor cells (line Ntva) or dopachrome tautomerase (DCT)-expressing melanoblasts (line DCTtva)

109 elated protein-1 (TYRP1/gp75) and dopachrome tautomerase (DCT/TYRP2) belong to a family of melanocyte

110 members of the MIF family MIF, D-Dopachrome Tautomerase (DDT) and DDT-like (DDTL) in a lung tissue d

111 nt work by others has described D-dopachrome tautomerase (DDT) as a functional homologue of MIF with

112 D-dopachrome tautomerase (DDT) is an enzyme that lacks physiologic su

113 e of MIF (and its family member D-dopachrome tautomerase (DDT)) in genitourinary cancers and how it c

114 identify the function of TenI as a thiazole tautomerase, describe the structure of the enzyme comple

115 (olfactory receptors, OR) and phenylpyruvate tautomerase/dopachrome isomerase activity (MIF and DDT g

116 4-Oxalocrotonate tautomerase (EC 5.3.2-; 4-OT), a hexamer consisting of 6

117 the quaternary structure of 4-oxalocrotonate tautomerase (EC 5.3.2; 4OT), and four analogues prepared

118 tophilus, and M. aeruginosa RLPs function as tautomerases/enolases in a methionine salvage pathway (M

119 It possesses unique tautomerase enzymatic activity.

120 F) is a proinflammatory mediator with unique tautomerase enzymatic activity; the precise function has

121 The expression of the dopachrome tautomerase gene (Dct) and its protein product, tyrosina

122 nt within an intron of the bovine dopachrome tautomerase gene.

123 base, Pro-1, of the enzyme 4-oxalocrotonate tautomerase has been mutated to Gly, Ala, Val, and Leu,

124 tyrosinase-related protein 1 and Dopachrome-tautomerase have lower protein levels in NPC1 deficient

125 hysiological investigations (e.g. dopachrome tautomerase in melanogenesis).

126 found at the active site of 4-oxalocrotonate tautomerase in the X-ray structure of the affinity-label

127 lection enabled the identification of a MIF2 tautomerase inhibitor R110.

128 receptor binding has prompted exploration of tautomerase inhibitors as potential biological antagonis

129 However, classical competitive MIF tautomerase inhibitors do not interfere with MIF functio

130 armacophore-switch to provide allosteric MIF tautomerase inhibitors that interfere with the MIF/AIF c

131 Three were found to be potent PfMIF tautomerase inhibitors with K(i) of approximately 40 nM;

132 mpounds are likely the most potent known MIF tautomerase inhibitors; the most active ones are more th

133 n inhibitory factor-2 (MIF-2 or D-dopachrome tautomerase) is a recently characterized second member o

134 MIF contains a catalytic site resembling the tautomerase/isomerase sites of microbial enzymes.

135 l-Dopachrome tautomerase (l-DCT), also called tyrosinase-related prot

136 Plants have orthologous MIF and D-dopachrome tautomerase-like (MDL) proteins that mimic some of the e

137 y have evolved from a short 4-oxalocrotonate tautomerase-like ancestor followed by gene duplication a

138 equence in each that had been annotated as a tautomerase-like protein but lacked Pro-1.

139 ro-1, but the sequence is not annotated as a tautomerase-like protein.

140 three MIF orthologs (termed MIF/d-dopachrome tautomerase-like proteins or MDLs) of the model plant Ar

141 We identify D-dopachrome tautomerase/macrophage migration-inhibitory factor-2 (MI

142 ore enabled identification of the allosteric tautomerase MIF inhibitor 6y with low micromolar potency

143 used in MIF wild-type mice (mif(+/+)) and in tautomerase-null, MIF gene knockin mice (mif (P1G/P1G)).

144 heart mitochondria an enzyme (OAT1) with OAA tautomerase (OAT) activity that converts enol-OAA to the

145 be activated by MITF, including dautochrome tautomerase, pMel 17/Silver and tyrosinase-related prote

146 on the mRNA levels of tyrosinase, dopachrome tautomerase, Pmel17, or MITF mRNA levels.

147 egulatory protein, exhibits a phenylpyruvate tautomerase (PPT) activity.

148 Phenylpyruvate tautomerase (PPT) has been studied periodically since it

149 ith Grm1 expression driven by the dopachrome tautomerase promoter.

150 gulation of a melanocyte-specific dopachrome tautomerase promoter.

151 Although the tautomerase site of D-DT and its homologue MIF are bioph

152 ecules that bind at the catalytically active tautomerase site of MIF and tested the complex for MIF b

153 olecule MIF inhibitors typically bind in the tautomerase site of the MIF trimer, often covalently mod

154 of 1.6 million compounds targeting the MIF-2 tautomerase site yielded several hits for potential cata

155 homology with significant differences in the tautomerase sites of the human and hookworm proteins.

156 of asymmetric arrangements of trimers in the tautomerase superfamily (TSF) adds structural diversity

157 The tautomerase superfamily (TSF) consists of more than 11,0

158 The tautomerase superfamily consists of three major families

159 at the evolution of new functions within the tautomerase superfamily could be quite facile, requiring

160 This is the first reported observation of a tautomerase superfamily member functioning by covalent c

161 d trans-3-chloroacrylic acid dehalogenase, a tautomerase superfamily member preceding MSAD in the tra

162 (MSAD) from Pseudomonas pavonaceae 170 is a tautomerase superfamily member that converts malonate se

163 ot exhibit the low-level activity of another tautomerase superfamily member, the heterohexamer trans-

164 d for Pro1 and the conserved arginine in all tautomerase superfamily members characterized thus far,

165 ino-terminal proline, conserved in all known tautomerase superfamily members, functioning as a genera

166 characterized member of a new family in the tautomerase superfamily that probably resulted from an i

167 acid dehalogenase (CaaD) are members of the tautomerase superfamily, a group of structurally homolog

168 minal region suggested a relationship to the tautomerase superfamily.

169 nce to implicate MSAD as a new member of the tautomerase superfamily.

170 4-OT isozyme and the founding member of the tautomerase superfamily.

171 e of three known enzymatic activities in the tautomerase superfamily.

172 t irreversible inhibitor of 4-oxalocrotonate tautomerase than is 2-OP suggest that Arg-39" and the or

173 f the functions assigned to MIF is that of a tautomerase that interconverts the enol and keto forms o

174 H from dopachrome is catalyzed by dopachrome tautomerase, that the melanogenic protein tyrosinase-rel

175 melanocortin receptor (MC1R), and dopachrome tautomerase (TRP-2).

176 tyrosinase-related protein-1 and dopachrome tautomerase/tyrosinase-related protein-2 and transactiva

177 that 4-OT might function as a 1,5-keto-enol tautomerase using 2-hydroxy-2,4-hexadienedioate.

178 One of those enzymes is 4-oxalocrotonate tautomerase, with which CaaD seems to share a common evo