ライフサイエンスコーパス: terminator

1 -antiterminator, antiterminator or intrinsic terminator.

2 etic block to invasion of the aptamer by the terminator.

3 se of the mRNA by puromycin, a peptide chain terminator.

4 reverse transcriptase to act as a DNA chain terminator.

5 ly fewer transcripts are able to bypass this terminator.

6 nd the other downstream of the transcription terminator.

7 function, acting as a non-obligate RNA chain terminator.

8 cture that functions as a factor-independent terminator.

9 emonstrated that one of these functions as a terminator.

10 nes, like NANOG, also have high Ssu72 at the terminator.

11 rth stem-loop functions as a rho-independent terminator.

12 e-stage oxidation to regenerate the aromatic terminator.

13 preferred SHM target upstream of the active terminator.

14 olding elements, or a weakened transcription terminator.

15 art sites and an intervening transcriptional terminator.

16 nator Override (TOV) genes that operate this terminator.

17 serotype-specific repeat unit domain, and a terminator.

18 ze polyubiquitin promoter and a heterologous terminator.

19 at the Rho utilization site of the lambdatR1 terminator.

20 or, T(IMM) , is a Rho-independent, intrinsic terminator.

21 cally incorporates the TFV part as the chain terminator.

22 ynthase 1 and subsequently served as a chain terminator.

23 ma(S)-dependent promoters but share the same terminator.

24 olor are seen on extended areas close to the terminator.

25 RNAP molecules after transcript release at a terminator.

26 the transposon functions as a transposition terminator.

27 d display Pol II pausing downstream from NRD terminators.

28 that exhibit transcriptional readthrough of terminators.

29 hose operating at constitutive Rho-dependent terminators.

30 fically recognized RNAs with Rho-independent terminators.

31 n the reverse orientation--and 265 synthetic terminators.

32 luding promoters, ribosome-binding sites and terminators.

33 tly more strong and reliable transcriptional terminators.

34 read-through transcription at Sen1-mediated terminators.

35 th Rho-dependent, as well as Rho-independent terminators.

36 direction predicted by the disparity of the terminators.

37 only be attributed to the disparity of line terminators.

38 nator during transcription through intrinsic terminators.

39 ased on the study of a small number of model terminators.

40 previous work with photochemically cleavable terminators.

41 fficiently at intrinsic and factor-dependent terminators.

42 sites, coding sequences and transcriptional terminators.

43 incorporate both reversible and irreversible terminators.

44 petition between reversible and irreversible terminators.

45 ormation of 3'-structural elements acting as terminators.

46 onstraint than promoters and Rho-independent terminators.

47 sh constitutive from regulated Rho-dependent terminators.

48 were after the Rho-independent transcription terminators.

49 te and define a broad class of transcription terminators.

50 and indirectly via readthrough of suboptimal terminators.

51 performance compared to two other reversible terminators, 3'-O-amino-TTP and 3'-O-azidomethyl-TTP.

52 e qPCR SYBR(R)Green technology targeting the terminator 35S pCAMBIA element.

53 Segments from the 5'-UTR, including the terminator 5'-stem-loop and Shine-Dalgarno blocking hair

54 Here, we report that ABA Signaling Terminator (ABT), a WD40 protein, efficiently switches o

55 core terminator stem are likely to increase terminator activity.

56 These analogs act as chain terminators after they are incorporated during RNA synth

57 novel two-piece assay that competes the anti-terminator against the aptamer, we directly monitor the

58 ion of 3 previously uncharacterized lncRNAs, TERMINATOR, ALIEN, and PUNISHER, specifically expressed

59 -density small dust particles near the lunar terminators, although later orbital observations yielded

60 polymerase III recognizes and pauses at its terminator, an oligo(dT) tract in non-template DNA, term

61 Deletion of the PapH terminator/anchor resulted in increased OM permeability,

62 of CstF (cleavage stimulatory factor) to the terminator and also the recruitment of the CstF and CPSF

63 it possible to assemble parts with repeated terminator and insulator sequences, and thereby create i

64 sequence redundancies--for example, repeated terminator and insulator sequences--that complicate reco

65 anscription that links the gene promoter and terminator and triggers initiation by RNA pol II.

66 ficity that favors cleavage at promoters and terminators and accounts for some of the correlation bet

67 ranscriptional read-through of selected gene terminators and because it physically interacts with the

68 es), from those that remain refractory (gene terminators and centromeres).

69 tructuring protein (H-NS) with Rho-dependent terminators and genetic interactions between hns and rho

70 ility in the structure of factor-independent terminators and identifies a mechanism for generation of

71 xtent of sequence diversity among functional terminators and the extent of mechanistic variation as a

72 binding dual-hairpin structures that overlap terminators and thus prevent transcription termination.

73 whose flanking regions including promoters, terminators and untranslated sequences could drive stabl

74 rminator clones compared with an inactivated terminator, and this region showed more single-stranded

75 ossovers increased toward gene promoters and terminators, and hot spots were associated with active c

76 Pcf11 localizes downstream from Nrd1 on NRD terminators, and its recruitment depends on Nrd1.

77 3'-amino nucleotides generally act as chain terminators, and no enzymatic pathway for their polymeri

78 acity, requirement of multiple promoters and terminators, and variable transgene expression levels.

79 The refolding kinetics of a bistable terminator antiterminator segment involved in the gene r

80 Most terminators are designed with 3'-O-blocking groups but a

81 Promoters or terminators are excluded from functional TUs by read-thr

82 Two types of bacterial transcriptional terminators are known to control gene expression.

83 Electron-rich aromatic terminators are required in both cases.

84 Rho-dependent terminators are sites of dissociation mediated by an RNA

85 pletion strain, we show that weak suboptimal terminators are the principle NusA substrates.

86 gh a few intrinsic prokaryotic transcription terminators are used routinely, termination efficiencies

87 4 genetic parts (promoters, ribozymes, RBSs, terminators) are parameterized and used to inform a math

88 53/37 and C11 to slow elongation and prevent terminator arrest.

89 First, we established a minimal terminator as 5T and 3A on the non-template and template

90 The results reveal the RNAP III terminator as an information-rich control element.

91 of the entire Rho-independent transcription terminator associated with the proK, proL and proM prima

92 TUs each have their own promoters; (iii) the terminators associated with the 3' ends of TUCs tend to

93 ct regulatory elements such as promoters and terminators associated with the novel expressed regions

94 ator, which allows formation of an intrinsic terminator (attenuator).

95 Switching of this terminator between active and inactive states dictates t

96 ay variability, and were seen at the morning terminator but not at the evening limb, which indicates

97 serving as an immediate polymerase reaction terminator, but not by a host-like high fidelity DNA pol

98 scripts ended at intrinsic (rho-independent) terminators, but most of the other transcripts seemed to

99 GT modules are separated from the GT99 chain terminator by a coiled-coil structure that forms a molec

100 to promoter sequences or as a transcription terminator by blocking the running RNAP.

101 Nascent pre-tRNAs released from a terminator by C37 mutants have shorter 3'-oligo(U) tract

102 shown that NS5B can efficiently remove chain terminators by a nucleotide-mediated excision reaction t

103 t base-selective incorporation of reversible terminators by DNA polymerases.

104 ecially important for understanding how such terminators can be regulated in response to specific sig

105 Readthrough of NusA-dependent terminators caused misregulation of genes involved in es

106 One of the two major terminator classes described so far is the Co-Transcript

107 ly(A) site were almost doubled in the active terminator clones compared with an inactivated terminato

108 on units (TUs), using different promoter and terminator combinations, that underlie state-switching.

109 Intrinsic transcription terminators consist of an RNA hairpin followed by a U-ri

110 These findings prove that terminators contribute to stereo matching, and constrain

111 We discovered that the terminator deletion mutant is highly resistant to neutro

112 Unlike Rho-dependent terminators described previously, where termination occu

113 at might be responsible for the detection of terminator disparity.

114 Intrinsic terminators dissociate transcription complexes without t

115 rial replication intermediates, an RNA chain terminator does not.

116 ough of transcription at the Rho-independent terminators downstream of araD and araE, leading to sign

117 Finally, terminators drive vergence even when the aperture is def

118 on, presumably through its action as a chain terminator during DNA replication.

119 s within the human beta-globin CoTC-mediated terminator element play a critical role in Pol II termin

120 th transcription termination at the oligo(T) terminator element, which forms a 3' oligo(U) tract on t

121 uired for gene loops that juxtapose promoter-terminator elements in a transcription-dependent manner.

122 n RNA processing signals as well as specific terminator elements located downstream of the poly(A) si

123 ithin seemingly random sequences, are strong terminator elements, and bioinformatics analysis confirm

124 -loops are particularly enriched over G-rich terminator elements.

125 Deletion of hasS or of the terminator eliminates CovR-binding sequences, relieving

126 By excluding terminators encoding such context-confounding elements,

127 id aggregation of the eukaryotic translation terminator eRF3/Sup35p, the [PSI (+)] prion, empowers ye

128 e, we demonstrate that these 3'-OH unblocked terminators exhibit superior enzymatic performance compa

129 ert Pol II into a viable target for the Xrn2 terminator exonuclease.

130 condary structures that might interfere with terminator folding kinetics or impact termination activi

131 that remdesivir acts as a delayed RNA chain terminator for MERS-CoV polymerase complexes.

132 ific anti-herpetic drug, acts as a DNA chain terminator for several human herpesviruses (HHVs), inclu

133 lopment of a complete set of four reversible terminators for application in NGS technologies.

134 nylation after Rho-independent transcription terminators for both mono- and polycistronic transcripts

135 2'-C-Me-DAPN-TP and 2'-C-Me-GTP were chain terminators for genotype 1b HCV-pol, and single nucleoti

136 tor I/II complexes function as transcription terminators for human snRNA genes with little, if any, r

137 conformation that is more accessible to anti-terminator formation.

138 in the context of aptamer collapse and anti-terminator formation.

139 ding an intermediate responsible for delayed terminator formation.

140 P are utilized in the processing of proL The terminator fragment derived from the endonucleolytic cle

141 lear which enzymes degrade the proK and proM terminator fragments.

142 t removing the Rho-independent transcription terminators from the primary valU and lysT transcripts.

143 on of anti-antiterminator, antiterminator or terminator function by competitor oligonucleotides in vi

144 tion of bacterial sequence features, such as terminators, functional sRNAs, and operons.

145 rovided with a Rho-independent transcription terminator, gene regulation was no longer PNPase-depende

146 n of transcriptional elements (promoters and terminators) generates new operons, restores the coordin

147 iting, including the possible utilization of terminator gRNAs that preclude the 3' to 5' progression

148 into the trigger helices and contacting the terminator hairpin after invasion of the hairpin in the

149 tight RNA binding by the ProQ NTD required a terminator hairpin of at least 2 bp preceding an 3' olig

150 NA and preventing the formation of pause and terminator hairpins.

151 ing, an equivalent role for the disparity of terminators has not been established.

152 stabilizes the Rho-RNA interactions at many terminators having suboptimal rut sites, thus making Rho

153 f the expression platform dominates, an anti-terminator helix is formed, and the transcription proces

154 entration preferentially stabilizes the anti-terminator helix, populating the ON state, relatively de

155 ercoming an otherwise stable transcriptional terminator if the bound tRNA is uncharged.

156 ts as an alternative substrate and RNA-chain terminator in primer-extension assays using a surrogate

157 t was reduced by a potential transcriptional terminator in promoter region 298 to 397 with a DeltaG =

158 ption complexes (ECs) can occur at intrinsic terminators in a 2- to 3-nucleotide window after transcr

159 o effect on antisense or sense Rho-dependent terminators in vivo.

160 yces pombe that cause pol III to readthrough terminators in vivo.

161 have measured the strengths of a library of terminators, including 227 that are annotated in Escheri

162 e substitutions that cause disruption of the terminator interfere with the regulatory function of UTR

163 uired for SHM, we knocked-in a transcription terminator into an Ig gene variable region in DT40 chick

164 ongation of prgQ transcripts past a putative terminator (IRS1) may be controlled by the interaction o

165 This terminator is based on 5-hydroxymethyl-2'-deoxyuridine t

166 The RNA polymerase III transcription terminator is flexibly accommodated within the transcrip

167 ated immediately upstream from the intrinsic terminator is necessary for subsequent degradation to oc

168 shed association of TFIIB E62K with the PMA1 terminator is restored by the Ssl2 H508R suppressor.

169 The function of this terminator is strongly attenuated by upstream mcc sequen

170 at promoter regions, nucleosome occupancy at terminators is strongly correlated with the orientation

171 sm of action of this hexameric transcription terminator, its regulation by different cis and trans fa

172 presence of a rho-independent transcription terminator just 28 bp upstream of the main translation s

173 acrophages and also predicts that rNTP chain terminators lacking a 3'-OH should inhibit HIV-1 reverse

174 h precise mutations in promoters, genes, and terminators, leading to altered carotenoid levels.

175 ase terminates transcription at an intrinsic terminator located in the intergenic region between the

176 ctinomycetemcomitans strain and identified a terminator located in the promoter region extending from

177 y expressed, is regulated by a Rho-dependent terminator located within its 5' leader region.

178 unveil widespread overlapping bidirectional terminators located between opposing gene pairs.

179 eotide analogs that act as nonobligate chain terminators may operate through a similar mechanism.

180 pt, there is a Rho-independent transcription terminator (named T (porA) in this study).

181 that the sequences at the distal tip of the terminator not directly involved in alternative secondar

182 disrupt interactions with transcription anti-terminator, NusA.

183 stages of axial elongation, not solely as a terminator of axial growth.

184 tract 5'-rUUUUUAU-3' from the tR2 intrinsic terminator of bacteriophage lambda.

185 ce of a role for the Integrator complex as a terminator of promoter-proximal RNA polymerase II during

186 roQ NTD specifically recognizes 3' intrinsic terminators of RNA substrates, and that the discriminati

187 Consistent with a structural role as terminators of secondary structure elements, substitutio

188 g hydrogen-bonding capabilities act as chain terminators of translesion DNA replication while analogs

189 t terminators, substantially more often than terminators of TUs that end inside a TUC; and (iv) the f

190 The availability of many terminators of varying strength, as well as an understan

191 the biologically active forms, act as chain terminators of viral DNA synthesis.

192 context of motion detection, the endings (or terminators) of 1-D features can be detected as 2-D feat

193 >4 mM) shift the equilibrium toward the anti-terminator on-state even in the presence of SAM.

194 larity (i.e. aptamer 'off-state' versus anti-terminator 'on-state').

195 The current study describes Terminator Operon Reporter (TOR), a new gene assembly te

196 genes through the tRNA-binding formation of terminator or antiterminator structures.

197 norbornene octyl ester with a CA-based chain-terminator or by the reaction of poly(ethylene oxide) wi

198 on or deletion of DNA encoding transcription terminators or a promoter modulates transcription rates.

199 either at specific DNA sequences, called the terminators, or by a nascent RNA-dependent helicase, Rho

200 ad-through assay, we identified trans-acting Terminator Override (TOV) genes that operate this termin

201 We found strong co-variation support for the terminator, P1, and anti-terminator stems in the purine

202 ssembly to construct repeating promoter-gene-terminator parts, we systematically varied gene expressi

203 ymerase (RNAP) to read through transcription terminators preceding bacteriophage late genes.

204 The Escherichia coli replication terminator protein (Tus) binds to Ter sequences to block

205 The replication terminator protein Fob1 of Saccharomyces cerevisiae is m

206 Here, we show that the replication terminator protein Fob1 of Saccharomyces cerevisiae prom

207 : elophase exit) complex and the replication terminator protein Fob1.

208 propose that XA10 is an inducible, intrinsic terminator protein that triggers programmed cell death b

209 A hexamer of the bacteriophage T4 tail terminator protein, gp15, attaches to the top of the pha

210 is process requires the binding of the polar terminator protein, Tus, to specific DNA sequences calle

211 nal communications between RNA pol I and the terminator protein.

212 terference by bi-directional transcriptional terminators proven to be highly efficient in in vitro tr

213 9, n = 31) better than models trained on all terminators (r = 0.67, n = 54).

214 While a DNA chain terminator rapidly blocks the labeling of mitochondrial

215 Using a sensitive terminator read-through assay, we identified trans-actin

216 3 and C37 and isolated many C37 mutants with terminator readthrough but no comparable C53 mutants.

217 Mutant RNA confirmed the presence of terminator readthrough transcripts.

218 No such terminator readthrough was observed in the mutant at the

219 ctivities: RNA oligo(U) 3'-end cleavage, and terminator readthrough.

220 of water as the chain transfer and/or chain terminator reagent, which is added at the end of the sal

221 ategy of creating 3'-OH unblocked reversible terminator reagents that, upon photochemical cleavage, t

222 tinct activities, that differentially affect terminator recognition and RNA 3' cleavage.

223 The terminator reduced mutations downstream of the poly(A) s

224 last rbcL, psbA, petD and rpoA genes and the terminator region of the Escherichia coli rrnB operon we

225 bryonic stem cells, with a minor peak in the terminator region.

226 ex and facilitating their recruitment to the terminator region.

227 nce of such sequences in 70% of the putative terminator regions (PTRs) genome-wide.

228 TFIIB also cross-links to terminator regions and is required for gene loops that j

229 ongly depleted of nucleosomes, but find that terminator regions are much less depleted than expected.

230 ase (MNase) suggests that yeast promoter and terminator regions are very depleted of nucleosomes, pre

231 d as the interaction of the promoter and the terminator regions of a gene during transcription, requi

232 onserved genic hotspots such as promoter and terminator regions of poly(A)-dependent genes.

233 D18 associates with the promoter, coding and terminator regions of target genes suggesting its functi

234 ce (UAS) and promoter elements, promoter and terminator regions, and regulatory and coding regions (g

235 l2, like TFIIB, associates with promoter and terminator regions, and the diminished association of TF

236 physical interaction of the promoter and the terminator regions.

237 y removing the Rho-independent transcription terminator represents a previously unidentified function

238 induced by the formation of transcriptional terminators represents a prime example for the coupling

239 ound that a bacterial protein, transcription terminator Rho of Clostridium botulinum (Cb-Rho), could

240 Bacterial Rho-independent terminators (RITs) are important genomic landmarks invol

241 tion mechanisms and the relationship between terminator sequence and steps in the termination mechani

242 We used these data to determine how the terminator sequence contributes to its strength.

243 tions (e.g. promoter, ribosome-binding site, terminator sequence), "devices" as any combination of pa

244 t of the HasS variations were located in the terminator sequences, suggesting that this region is und

245 iboswitch by extending alignments to include terminator sequences.

246 monly used Hsp70A-RBCS2 (AR) hybrid promoter/terminator sequences.

247 Sanger dideoxy terminator sequencing allows for rapid development and i

248 s and allowed robust dideoxynucleotide chain terminator sequencing from as little as 10 genome equiva

249 tion followed by Illumina NextSeq reversible terminator sequencing with DNA selection by amplificatio

250 The integrated tag is coupled to a generic terminator shielding the tagged gene from the co-inserte

251 ulting systematically measured collection of terminators should improve the engineering of synthetic

252 Lightning Terminators show maximum incorporation rates (k(pol)) th

253 ltifunctional proteins that bind to specific terminator sites (Ter) and cause polar termination of tr

254 nd that structures extending beyond the core terminator stem are likely to increase terminator activi

255 eotide CAUAGC to form either a pseudoknot or terminator stem.

256 an otherwise classic intrinsic transcription terminator stem.

257 ion support for the terminator, P1, and anti-terminator stems in the purine riboswitch by extending a

258 a specific UTR1 conformation that favors the terminator structure in Mn(2+)-replete condition.

259 required for the full formation of the anti-terminator structure, and that higher concentrations of

260 RNA fragments that contain the transcription terminator structure.

261 e 3' ends of TUCs tend to be Rho-independent terminators, substantially more often than terminators o

262 e-subunit complexes except for the chaperone-terminator subunit (PapDH) and has a catalytic role in d

263 describe a role for SCAF4 and SCAF8 as anti-terminators, suppressing the use of early, alternative p

264 We also find that the transcription terminator, T(IMM) , is a Rho-independent, intrinsic ter

265 include the P(LIT) promoter and the immunity terminator, T(IMM) .

266 NusA-dependent terminators tend to have weak hairpins and/or distal U-t

267 ptic (kissing) interactions between pairs of terminator (Ter) sites that initiated recombination in r

268 mall RNA, HasS, an intrinsic transcriptional terminator that inefficiently terminates HasS, permittin

269 hese errors are monitored by a transcription terminator that is placed between the synthetic gene and

270 upstream of the TH or point mutations in the terminator that preserve TH stability affect termination

271 led a collection of 61 natural and synthetic terminators that collectively encode termination efficie

272 ence elements such as standard promoters and terminators that interfere with homologous recombination

273 arts (promoters, ribosome binding sites, and terminators) that are functionally separated by spacer p

274 xtremely high-altitude plumes at the Martian terminator (the day-night boundary) at 200 to 250 kilome

275 coassociates with both the promoter and the terminator, the inactivation of Ssu72 leads to increased

276 ontrol element and a furan as a nucleophilic terminator, the key structural features of hatJ were rap

277 cleoside RT inhibitors (NRTIs) are DNA chain terminators, the nucleotide-competing RT inhibitor (NcRT

278 p structure of rho-independent transcription terminators, thus avoiding intact genes.

279 oeae functions as an intrinsic transcription terminator to generate non-stop mRNA.

280 ) transporter, which forms a Rho-independent terminator to implement transcription termination with a

281 is promoting the transfer of RNAPII from the terminator to the promoter for reinitiation of transcrip

282 of an A-rich sequence on the 5' side of the terminator to uridines strengthened the binding of sever

283 ach other by placing ribozymes downstream of terminators to block nuclear export of messenger RNAs re

284 Intrinsic terminators trigger pausing on weak RNA-DNA hybrids foll

285 ybrids are found at both promoters (TSS) and terminators (TTS) of highly expressed genes.

286 /IL-24, we created a tropism-modified cancer terminator virus (Ad.5/3-CTV), which selectively replica

287 EG-E1A-mda-7; also called Ad.5/3-CTV (cancer terminator virus)].

288 We found that the human beta-globin terminator was an efficient inhibitor of downstream tran

289 on downstream of the predicted transcription terminator was dose dependent and specific to c-di-GMP b

290 Moreover, a subclass of weak non-canonical terminators was identified that completely depend on Nus

291 sampling on purine riboswitch sequences with terminators we found that these sequences appear to have

292 V) was identified as an efficient reversible terminator, whereby, sequencing feasibility was demonstr

293 t, a mutation within the rho transcriptional terminator, which defines an alternative adaptive pathwa

294 '-OH unblocked nucleotides, called Lightning Terminators, which have a terminating 2-nitrobenzyl moie

295 n interacted with the 5' splice site and the terminator with the 3' splice site.

296 describe a novel 3'-OH unblocked reversible terminator with the potential to improve accuracy and re

297 %) of both antisense and sense Rho-dependent terminators with lower C/G ratio sequences.

298 I subunits or insertion of an ectopic Pol I terminator within the adjacent rDNA gene.

299 es, where each gene has its own promoter and terminator, within the same T-DNA.

300 Within this library we found 39 strong terminators, yielding >50-fold reduction in downstream e