ライフサイエンスコーパス: thaw

1 d greater nutrient availability to plants at thaw.

2 sed pre-freeze and 0, 30, 60 and 90 min post-thaw.

3 easons, accelerated snowmelt, and permafrost thaw.

4 xperiencing rapid evolution after permafrost thaw.

5 terrestrial sources arising from permafrost thaw.

6 ings associated with near-surface permafrost thaw.

7 climate change causes widespread permafrost thaw.

8 by the amount of C that accumulated prior to thaw.

9 s all treatments, despite different rates of thaw.

10 inducing higher soil moisture during spring thaw.

11 latitudes, including near-surface permafrost thaw.

12 nderstory or tree canopy shading in reducing thaw.

13 sociated with CO2-C release after permafrost thaw.

14 to total annual emission than that of spring thaw.

15 dient and cannot prevent carbon release with thaw.

16 etention of volatile components after freeze-thaw.

17 ands are expanding rapidly due to permafrost thaw.

18 igh pressure-assisted extraction, and freeze-thaw.

19 50% at sites strongly affected by permafrost thaw.

20 barctic peatlands increase as the permafrost thaws.

21 protein denaturation in drip due to freezing-thawing.

22 ges in potency occurring during freezing and thawing.

23 frost soils are now within ~0.5 degrees C of thawing.

24 insight on protein denaturation in freezing-thawing.

25 red by a comparable mechanism as in freezing-thawing.

26 salmon flesh as a marker of salmon freezing/thawing.

27 (0.88 +/- 0.03 mg m(-2) hr(-1) ) than spring thaw (0.48 +/- 0.04 mg m(-2) hr(-1) ).

28 is significantly longer than that of spring thaw (20.94 +/- 7.79 days), which predominates the much

29 rcharge-overdischarge (3-1.6 V) and freezing-thawing (25-250 degrees C) incidents.

30 mg m(-2) year(-1) ) than that during spring thaw (307.39 +/- 46.11 mg m(-2) year(-1) ).

31 tants for cod fish mince subjected to freeze-thaw abuse.

32 imizing salt extractable protein from freeze-thaw abused fish mince, providing similar or better cryo

33 Reco , GPP, and NEE increased linearly with thaw across all treatments, despite different rates of t

34 nt the star fruit firmness was maintained on thawing after 60 days of storage.

35 ces to the atmosphere for a decade following thaw, after which post-thaw bog peat accumulation return

36 rest) and thawed permafrost bogs, ranging in thaw age from young (<10 years) to old (>100 years) from

37 As the cryosphere melts and thaws, alpine lakes and streams will experience major ch

38 Permafrost thaw also stimulates plant growth, which could offset C

39 rise in the future, ice-rich permafrost may thaw, altering soil topography and hydrology and creatin

40 y investigates CH(4) emissions during spring thaw and autumn freeze using eddy covariance CH(4) measu

41 CO2 sources associated with deep permafrost thaw and cold season respiration expected over the next

42 ctron transfer components lost during freeze/thaw and correcting for variable permeabilization of mit

43 As these ecosystems warm, the thaw and decomposition of permafrost is expected to rele

44 Soil warming caused rapid permafrost thaw and increased ecosystem respiration (Reco ), gross

45 nd bioaccumulation in lakes, while increased thaw and surface water flow will likely result in higher

46 ics in terms of pasting, rheological, freeze-thaw and swelling behaviour, were investigated.

47 nd shipped to the patient where they will be thawed and administered.

48 treatment trial in Birmingham, Alabama, were thawed and grown in culture.

49 lantation, frozen samples of cord blood were thawed and the purity of viable nucleated cells was incr

50 site of irreversible injury during freezing/thawing and cryopreservation of cells, but the underlyin

51 n environmental conditions upon freezing and thawing and demonstrates the enormous complexity of free

52 , glycine addition during both vitrification/thawing and maturation further enhanced the oocyte quali

53 rmation was influenced by storage time after thawing and not by the time after slaughter.

54 Freeze-thawing and pasteurization increased the milk lipolysis

55 Repeated thawing and re-freezing up to three times did not change

56 marate increased until the 3rd-5th day after thawing and then gradually decreased, reaching zero afte

57 As climate warms, permafrost thaws and soil organic matter becomes vulnerable to grea

58 traffic compaction, swell/shrink and freeze/thaw) and biological (e.g. plant root growth, soil micro

59 mples that had experienced additional freeze-thaw, and increased standing times of 120 and 240 min wi

60 ssing delay, processing method, freezing and thawing, and sample volume on pcfDNA.

61 ity criteria for inclusion: WAKE-UP, EXTEND, THAWS, and ECASS-4.

62 Early stages of thaw are characterized by high DOC concentrations, high

63 thaw, indicating that higher Reco in deeply thawed areas during summer months was balanced by GPP.

64 tmosphere and are increasingly vulnerable to thaw as high-latitude temperatures warm.

65 frozen at -80 degrees C for 10 min and then thawed at 25 degrees C for 5 min before SPME extraction

66 ared highly amorphous, and when subsequently thawed at slow rates (6.2 degrees C min(-1) and below) i

67 0.5 mL straws, 2 cm above LN for 4 min then thawing at 37 degrees C for 1 min.

68 ctic, glaciers are melting and permafrost is thawing at unprecedented rates, releasing not only water

69 s approach was used to distinguish fresh and thawed Atlantic salmon.

70 ip between an ice-free Arctic and permafrost thawing before 0.4 Ma.

71 s antioxidant capacity and texture of frozen/thawed blueberries.

72 or a decade following thaw, after which post-thaw bog peat accumulation returned sites to net C sinks

73 tundra communities, RAF connectivity to the thaw boundary was ubiquitous.

74 In general NEE increased with thaw, but was more strongly correlated with plant biomas

75 We found that upon thaw, C loss of the forest peat C is equivalent to 30% o

76 We found that upon thaw, C loss of the forest peat C is equivalent to ~30%

77 It highlights the variability of post-thaw carbon dynamics in boreal and arctic ecosystems.

78 rm in permafrost peatlands, where permafrost thaw caused a fivefold increase in emissions (0.56 +/- 0

79 It was found that salmon freezing/thawing caused a significant increase in the concentrati

80 ificantly augment the permeability of frozen-thawed coal masses.

81 Permafrost thaw could induce substantial carbon (C) emissions to th

82 acids significantly increased in the freeze-thawed crude fecal samples, suggesting a release of micr

83 "Series 1" compared the effects of freshly thawed cryopreserved umbilical cord-mesenchymal stem/str

84 Our results demonstrate that freshly thawed cryopreserved xeno-free human umbilical cord-mese

85 Post-thaw culture functional performance was also influenced

86 es resulting in increased warming and freeze-thaw cycle (FTC) frequency pose great ecological challen

87 Freeze-thaw cycle dynamics play a critical role in controlling

88 The timing of the seasonal freeze-thaw cycle of arctic lakes affects ecological processes

89 miRNA levels, and show that a single freeze/thaw cycle of plasma dramatically increases the number o

90 thus examined the effects of a single freeze/thaw cycle on microparticles (MPs) and miRNA levels, and

91 the presence of thiolated DNA after a freeze-thaw cycle.

92 One additional freezing-thawing cycle at slow freezing rate caused appearance of

93 ity of the wines before and after the freeze-thawing cycle.

94 The effect of freeze-thaw cycles (0 or 1) and time to erythrocyte removal (30

95 r 14 days at 37 degrees C), iterative freeze-thaw cycles (3.4-fold post four-cycles), and lyophilizat

96 sion was stable against the effect of freeze-thaw cycles (no phase separation observed).

97 me, storage-temperature, and repeated freeze-thaw cycles on circulating BDNF concentrations was evalu

98 s stored beyond 6 months and repeated freeze-thaw cycles should be avoided.

99 ation with coal permeability, and the freeze-thaw cycles significantly augment the permeability of fr

100 of storage conditions and consecutive freeze/thaw cycles was determined.

101 ctylus) muscle subjected to different freeze-thaw cycles was investigated.

102 nces in clotting times, the number of freeze-thaw cycles, and different trypsin/protein ratios.

103 e effects of freezing time, number of freeze-thaw cycles, and the moisture content of coal were studi

104 o cracking owing to drying shrinkage, freeze-thaw cycles, delayed ettringite formation, reinforcement

105 50 degrees C, or undergoing repeated freeze-thaw cycles, were compared with freshly extracted sample

106 s above -80 degrees C and consecutive freeze/thaw cycles.

107 tor tube, storage-time, and number of freeze-thaw cycles.

108 om temperature for 4 h or up to three freeze-thaw cycles.

109 e reproducibly performed over several freeze-thaw cycles.

110 ing nonrefrigerated transportation or freeze-thaw cycles.

111 cture with higher digestibility after freeze-thaw cycles.

112 tressed by low pH, heat, and multiple freeze-thaw cycles.

113 torage at room temperature, and three freeze-thaw cycles.

114 d to determine the effect of multiple freeze-thaw cycles.

115 Comparison to freezing time, freeze-thaw cycling caused much more damage to the coal strengt

116 ed by micro-flow imaging (MFI) during freeze-thaw cycling in phosphate buffered solutions.

117 ntrifugation) and thermal stressors (freeze, thaw cycling).

118 or three different physical stresses: freeze-thaw cycling, heating, and agitation.

119 iability and tissue architecture from freeze-thaw cycling.

120 The third variable studied, freeze-thaw damage resulting from high moisture content, was re

121 stimated from the observed plant biomass and thaw depth increases in tundra ponds over the past 40 ye

122 stimated from the observed plant biomass and thaw depth increases in tundra ponds over the past 40 ye

123 r of A. fulva CH4 flux while water depth and thaw depth were copredictors for C. aquatilis CH4 flux.

124 13, to quantify changes in plant biomass and thaw depth, and used these to estimate species-specific

125 d declines in soil moisture and temperature, thaw depth, shrub height, and foliar nitrogen content, i

126 rom tissue and subjecting it to freezing and thawing did not significantly affect (P > 0.05) its perm

127 Following thaw, dissolved organic matter (DOM) is a potentially im

128 lysis of the labeled fractionated permafrost thaw DOM directly showed carboxyl-rich alicyclic molecul

129 on this by influencing the maximum depth of thaw each summer (active-layer thickness; ALT), but a qu

130 sults demonstrate the importance of indirect thaw effects on CO2 flux: plant growth and water table d

131 almon by-products to pH-adjustment or freeze/thawing efficiently released the emulsified oil at 4 deg

132 greenhouse gas concentrations as permafrost thaw exposes immense stores of frozen carbon (C) to micr

133 Permafrost in the Arctic is thawing, exposing large carbon and nitrogen stocks for d

134 nal stressors including: desiccation, freeze/thaw, exposure to high temperatures, osmotic shock, as w

135 The Freeze-thaw (F-T) stability and turbidity of GCWS were also hig

136 at aspartate was formed in the flesh of only thawed fish after the second day of storage.

137 of some metabolites in reference and frozen-thawed fish during its storage.

138 e double-freeze technique (freeze for 3 min, thaw for 5 min, and freeze again for 3 min), and LLETZ (

139 In boreal lowlands, thawing forested permafrost peat plateaus ('forest') lea

140 es (delayed freezing up to 24 h and repeated thawing/freezing for up to three cycles) affects the mea

141 When cytoplasmic extracts prepared by freeze/thaw from a control strain were fractionated by gel filt

142 nctionally and structurally seven days after thaw from cryopreservation.

143 explored the relationships between root and thaw front fungal composition and plant uptake of a (15)

144 ERM and ECM shrubs associate with RAF at the thaw front providing evidence for potential mycelial con

145 currence of particular RAF in both roots and thaw front soil was positively correlated with (15) N re

146 elow the maximum rooting depth in permafrost thaw-front soil in tussock and shrub tundra communities.

147 ver, these block copolymer worms enable post-thaw gelation by simply warming to 20 degrees C.

148 to study matrix mobility in fresh and freeze-thawed gelled yolk.

149 rbon sink is effectively destroyed along the thaw gradient and cannot prevent carbon release with tha

150 bon using a space-for-time approach across a thaw gradient in Abisko (Sweden), where wetlands are exp

151 bilization of both iron and carbon along the thaw gradient.

152 Varied thaw history was reported to be the main driver of soil

153 The stool samples were thawed, homogenized, and used for 9 different quantitati

154 We conclude that, due to permafrost thaw, hydrocarbon-rich areas, prevalent in the Arctic, m

155 n of DOM across sites at different stages of thaw in a discontinuous permafrost area of North Siberia

156 remain about C dynamics following permafrost thaw in boreal peatlands.

157 , but this change could be explained by slow thaw in Control areas.

158 n nongrowing season and peaked during spring thaw in each year.

159 As thaw in Soil warming continued to increase linearly, gro

160 hange in a landscape subjected to permafrost thaw in unburned dominant forest types (paper birch and

161 this test, wine samples are frozen and then thawed in controlled conditions.

162 ic proteins of pork loins caused by freezing-thawing in relation to freezing rate.

163 strongly correlated with plant biomass than thaw, indicating that higher Reco in deeply thawed areas

164 d and fractal dimension analyses, how freeze-thaw induced fractures in the coal was quantitatively an

165 Freeze-thaw induced fracturing coal by liquid nitrogen (LN2) in

166 e sporadic permafrost zone of North America, thaw-induced boreal forest loss is leading to permafrost

167 Thus, thaw-induced CH4 emission increases likely exert a posit

168 ges in landscape functioning associated with thaw-induced collapse-scar bog ('wetland') expansion.

169 Here, we quantify the thaw-induced increase in CH4 emissions for a boreal fore

170 reases in acetate fermentation expected from thaw-induced increases in SOC availability.

171 e (by approximately 90%) but did not prevent thaw-induced N2O release, whereas waterlogged conditions

172 release from Arctic soils due to permafrost thawing is known to be substantial, but growing evidence

173 revent carbon mobilization during permafrost thaw, is poorly understood.

174 limate change, because when frozen sediments thaw it unlocks soil organic carbon.

175 Freeze-thaw, known as an unfavorable lysis method resulting in

176 rates would decrease over time and submerged thaw-lake taliks would freeze; therefore, no CH4 release

177 emical and fatty acid stability of fresh and thawed lamb leg chops, frozen stored for 3, 6 and 9month

178 e recommended for preserving either fresh or thawed lamb.

179 by measuring electrolyte leakage from freeze-thawed leaf discs.

180 Using Prodigy, thawed leukopak cells were enriched for CD4(+) and CD8(+

181 borated from frozen (-20 degrees C/20 weeks)/thawed longissimus dorsi muscles (F) were compared with

182 role of protein denaturation in formation of thaw loss is currently not well understood.

183 st freezing, slow freezing caused 28% larger thaw loss, decreased water-holding capacity of myofibril

184 less severe protein denaturation and reduced thaw loss.

185 p platform for performing single-cell freeze-thaw lysis directly toward 3' mRNA sequencing.

186 myofibrillar protein denaturation in frozen-thawed meat.

187 he addition of ffEVs to vitrification and/or thawing media enhanced the ability of frozen-thawed oocy

188 The freeze-thaw method clearly improved the detection of volatile c

189 detection of volatiles in insects, a freeze-thaw method was applied to insect samples before the HS-

190 A careful thawing method was used to collect the upper parts of th

191 Freezing-thawing minced pork reduced water-holding of myofibrils

192 minimizes visceral fat delocalization after thaw-mounting of tissue sections.

193 Ultra was performed on thawed NPAs and IS specimens individually.

194 The evaluation of freshness and freeze-thawing of fish fillets was carried out by assessment of

195 Thermokarst is the process whereby the thawing of ice-rich permafrost ground causes land subsid

196 Both heavy precipitation events and thawing of permafrost are increasing the net transfer of

197 l waters through increases in precipitation, thawing of permafrost, and changes in vegetation.

198 Thawing of PF-C must also have brought about an enhanced

199 of summer Arctic sea ice will accelerate the thawing of Siberian permafrost.

200 he impacts of thermokarst (abrupt permafrost thaw) on microbial structure and function remains limite

201 thawing media enhanced the ability of frozen-thawed oocytes to resume meiosis.

202 his share may increase if ongoing permafrost thaw opens new pathways.

203 ult stem cells, cells killed by freezing and thawing or a chemical inducer of the innate immune respo

204 cine supplementation in either vitrification/thawing or maturation medium significantly improved the

205 hout PRF lysates obtained by repeated freeze-thawing or the secretome of PRF membranes, termed PRF co

206 (1/5 fresh, 1/4 papain-treated, 0/17 frozen-thawed; P = 0.10).

207 rapidly-warming temperatures and lengthening thaw periods.

208 in forested permafrost plateaus (forest) and thawed permafrost bogs, ranging in thaw age from young (

209 As Arctic soils warm, thawed permafrost releases nitrogen (N) that could stimu

210 climate change through carbon releases from thawing permafrost and higher solar absorption from redu

211 cycling in tandem with changing inputs from thawing permafrost and industrial activity.

212 The bog (thawing permafrost and low radiative forcing signature)

213 na Flats in central Alaska for centuries, as thawing permafrost collapses forests that transition to

214 making it difficult to predict how inputs of thawing permafrost DOM may alter its photodegradation.

215 stimate potential future releases of Hg from thawing permafrost for low and high greenhouse gas emiss

216 roduction and release of methane (CH4 ) from thawing permafrost has the potential to be a strong sour

217 s) pup (specimen YG 648.1) was discovered in thawing permafrost in the Klondike goldfields, near Daws

218 Thawing permafrost opens pathways for this CH4 to migrat

219 ical for carbon budgets in the Arctic, where thawing permafrost soils increase opportunities for DOC

220 Thawing permafrost soils will change the chemical compos

221 e and late-Pleistocene) carbon released from thawing permafrost soils, but the magnitude of these sou

222 summer from melting snow and ice as well as thawing permafrost, contrasting earlier notions of limit

223 ocused on atmospheric release of carbon from thawing permafrost, yet overlooked waterborne release pa

224 d to detect the onset of carbon release from thawing permafrost.

225 ries-to millennia-old soils that extend into thawing permafrost.

226 ver than the release of pre-aged carbon from thawing permafrost.

227 arbon and mercury from melting polar ice and thawing permafrost; new funding schemes and regulations;

228 Within 5 years of permafrost thaw, plants actively incorporate newly available N into

229 urine and supernatant and between fresh and thawed plasma and urine after 24 weeks at -80 degrees C.

230 Permafrost thaw ponds of the warming Eastern Canadian Arctic are ma

231 tials in high-dissolved organic matter (DOC) thaw ponds on Bylot Island (BYL) and a low-DOC oligotrop

232 ltry, visually indistinguishable from frozen-thawed poultry, presents an attractive target for adulte

233 t also devitalized MFAT (DMFAT) (by freezing/thawing procedure) were able to deliver and release sign

234 gest that uncertainty associated with freeze-thaw processes as well as soil textural effects on soil

235 ratures cannot completely reflect the freeze-thaw processes in deeper soil layers and appears to have

236 Here, we analyze freeze-thaw processes through in situ CO(2) and CH(4) fluxes in

237 increased community heterogeneity along the thaw progression.

238 (P < 0.001), storage vessel (P = 0.002) and thaw rate (P = 0.03).

239 es (2, 4 or 8 cm above liquid nitrogen; LN), thaw rates (37 degrees C for 1 min or 42 degrees C for 2

240 In the initial stages of thaw, Reco , GPP, and NEE increased linearly with thaw a

241 e, culturing of the cells in vitro, freezing/thawing, reintegration into a recipient embryo and the d

242 oth related to minimum temperature and, upon thawing, related to vapor pressure deficit and soil temp

243 ponses of microbial functional potentials to thaw-related soil and plant changes and provides informa

244 eveloped MSC that retain >95% viability upon thawing, remain responsive to inflammatory signals, and

245 re, drip loss and colour were evaluated with thawed samples.

246 ed re-emissions of previously stored Hg from thawing sea-ice, glaciers, and permafrost.

247 We found that observed thaw seasons are 10-30% shorter than those assumed in pr

248 , across a hydrologic continuum (composed of thaw seeps, lake/ponds, and a wetland) to identify Hg me

249 ties and functional genes along a permafrost thaw sequence (1, 10, and 16 years since permafrost coll

250 by changes in substrate properties along the thaw sequence.

251 labile and stable C decomposition along the thaw sequence.

252 ysts were cryopreserved and a delayed frozen-thawed single blastocyst transfer was done.

253 Particularly, the moderately thawed site contained microbial communities with the hig

254 Compared with the minimally thawed site, the number of detected functional gene prob

255 th profile at the moderately and extensively thawed sites decreased by 25% and 5%, while the communit

256 nt of minimally, moderately, and extensively thawed sites.

257 changing nitrogen (N) availability in these thawing soil profiles.

258 Nonsummer CO2 loss in warmer, more deeply thawed soils exceeded the increases in summer GPP, and t

259 er snow enhanced CH4 production within newly thawed soils, responding mainly to soil warming rather t

260 The percentage of viable frozen-thawed sperm (%ViableSperm) determined by flow cytometry

261 we measured and correlated the DFI of frozen-thawed sperm from 83 unique mutant mouse strains with sp

262 with either fresh, papain-treated or frozen-thawed spermatozoa.

263 high acid and shear resistance, high freeze-thaw stability and improved gel texture.

264 rees C and -20 degrees C for 2 weeks (freeze/thaw stability).

265 sites representative of distinct permafrost thaw stages at a palsa mire in northern Sweden.

266 h harsh conditions, including extreme freeze-thaw stress.

267 to peracetic acid, refrigeration and freeze-thaw stresses.

268 eragrams (1 Tg = 10(6) tons) of methane from thawing subsea permafrost on shallow continental shelves

269 w) ice recrystallization was observed during thaw suggesting mechanical disruption of the frozen cell

270 f increasing importance in permafrost as the thawed surface region ("active layer") deepens.

271 ion of SMP resulted in an increase in freeze-thaw syneresis and reduction in starch granule size.

272 A controlled freeze-thawing test for wines is proposed to predict the deioni

273 recommended operating conditions, the freeze-thawing test gives reproducible results, which are betwe

274 The passive version of freeze-thawing test seems to be an expedite and reliable method

275 butary of Last Chance Creek during hydraulic thawing that exposed the permafrost sediment in which it

276 If this protective layer thaws, these soils are predicted to warm up at 1.5x to 4

277 The effect of freezing holding time and thawing time on the predicted deionization degree was in

278 xes is driven with satellite observations of thaw timing and duration.

279 organoids can be grown from flash-frozen and thawed tissue and from bulk tissues slowly frozen in DMS

280 le part of the PP-C will degrade at point of thaw to CO(2) and CH(4) to directly amplify global warmi

281 effect on CH(4) emissions from early spring thaw to late autumn freeze.

282 A "freeze-thaw" treatment on seeds of red vinifera cultivars at ve

283 der heat, dehydration-rehydration and freeze-thaw treatments.

284 ls exceeded the increases in summer GPP, and thawed tundra was a net annual CO2 source.

285 Our model results indicate that permafrost thaw turned these peatlands into net C sources to the at

286 ximal concentration on the 3rd-5th day after thawing (up to 3.8 mg in 100 g of muscle) and gradually

287 red for channel freezing (valve closure) and thawing (valve opening) were measured.

288 Freeze-thaw valves (FTVs) can provide a low cost, low complexit

289 amage caused by each of the different freeze-thaw variables were empirically regressed.

290 To evaluate the different freeze-thaw variables which modify the mechanical properties of

291 differences in CH(4) emissions during spring thaw versus autumn freeze to accurately estimate CH(4) s

292 right ovary was removed, the left ovary was thawed/warmed, and its vessels were anastomosed to the r

293 tured the functional response of CO2 flux to thaw, water table depth, and plant biomass.

294 During permafrost thaw, water-logging and O(2) limitation lead to reducing

295 sed protocol (30 min standing time, 0 freeze-thaw) were used, resulting in high diagnostic accuracy (

296 ting the effect of temperature, freezing and thawing, where the exclusion of salt and AuNPs by the gr

297 o result in increases of snowpack and deeper thaws, which could increase this ecosystem respiration d

298 Vitrified oocytes were thawed with or without ffEVs, assessed for survival, in

299 ed directly for infusion into patients after thawing with no further processing.

300 nutrients to the Arctic Ocean as permafrost thaws, yet few studies have quantified groundwater input