ライフサイエンスコーパス: thermal unfolding

1 structures, and provided resistance against thermal unfolding.

2 s = 11.8 kcal/mol) and undergoes cooperative thermal unfolding.

3 t cell and which can be mimicked in vitro by thermal unfolding.

4 affinity and makes Red Broccoli resistant to thermal unfolding.

5 FGF1 is known to aggregate when subjected to thermal unfolding.

6 d also from the conformations sampled during thermal unfolding.

7 s elegans and also subjected this protein to thermal unfolding.

8 within the MT-binding domain of SNPH against thermal unfolding.

9 the possibility of an intermediate state in thermal unfolding.

10 a different mechanism that does not involve thermal unfolding.

11 stallography, fluorescence spectroscopy, and thermal unfolding.

12 strains to both guanidine hydrochloride and thermal unfolding.

13 tiary structure, as indicated by cooperative thermal unfolding and a well-dispersed NMR spectrum.

14 small heat-shock protein family, suppresses thermal unfolding and aggregation of the myosin II molec

15 NaCl concentrations, were stabilized against thermal unfolding and aggregation triggered by high HOCl

16 , we combine equilibrium NMR measurements of thermal unfolding and long molecular dynamics simulation

17 kinetic stability of discoidal lipoproteins, thermal unfolding and refolding studies were carried out

18 A scan rate dependence and hysteresis in the thermal unfolding and refolding was observed for all pro

19 With respect to thermal unfolding and the stabilization by APS, PPS-1 be

20 i has been determined using a combination of thermal unfolding and urea denaturation experiments.

21 All of the mutants display sigmoidal thermal unfolding and urea-induced unfolding curves.

22 stability of the cross-linked structures to thermal unfolding, and the sites of peptide cross-linkin

23 anning calorimetry (DSC) curves for the HisJ thermal unfolding are well described by a scheme of equi

24 The role of thermal unfolding as it pertains to thermodynamic proper

25 Apo MMP3 is remarkably stable to thermal unfolding as monitored by CD; thus the metal ion

26 s of secondary and tertiary structures after thermal unfolding at 90 degrees C, as well as preventing

27 s, undergo a relatively sharp and reversible thermal unfolding at approximately 60 degreesC.

28 pectroscopy to investigate the site-specific thermal unfolding at seven different locations in the de

29 We conclude that (1) a three-state thermal unfolding behavior appears to be conserved among

30 omplexes by sparse-matrix screening of their thermal unfolding behavior in the presence of various bu

31 t mutants are practically identical, and the thermal unfolding behavior is very similar.

32 subtly altered the protein-PEG interface and thermal unfolding behavior, providing insight into ratio

33 CD studies show no difference in thermal unfolding between beta-SmTm and DeltaN-betaSmTm;

34 is well-folded and highly resistant against thermal unfolding but aggregates at elevated temperature

35 nge in negative ellipticity at 222 nm during thermal unfolding, but in the near-UV circular dichroism

36 nding stabilizes the protein as monitored by thermal unfolding by CD (50.7-54.8 degrees C) and by dif

37 , the compact folding intermediate formed by thermal unfolding can be protected against proteolysis a

38 content by 5-20% and to a large increase in thermal unfolding cooperativity.

39 ing temperature and the reversibility of the thermal unfolding curve (as measured by CD spectroscopy)

40 Further, BHBox showed a sigmoidal thermal unfolding curve with a per-residue van't Hoff en

41 ence of helical structure and to measure the thermal unfolding curve.

42 ree C shift in the far UV circular dichroism thermal unfolding curve.

43 he method includes the automated analysis of thermal unfolding curves based on a biophysical unfoldin

44 In contrast, thermal unfolding curves determined via CD indicate that

45 2,2, 2-trifluoroethanol) back to water, the thermal unfolding curves have been measured by circular

46 The change in the apparent cooperativity of thermal unfolding curves in concentrated TFE solutions r

47 folding predicts some dispersion of measured thermal unfolding curves of individual residues at pH5.3

48 Thermal unfolding curves of the five 13-residue peptides

49 halpy of helix formation calculated from the thermal unfolding curves varies widely among the five pe

50 um chloride induces cold denaturation in the thermal unfolding curves, providing a reasonably well-de

51 ure is formed cooperatively, as indicated by thermal unfolding curves.

52 d not only to be comparable to previous FTIR thermal unfolding data but also to have a denatured-stat

53 delta Cp, was measured by global analysis of thermal unfolding data collected at a number of guanidin

54 s fit should be applicable to isothermal and thermal unfolding data for all proteins with similar com

55 Thermal unfolding data on 158 backbone and side-chain pr

56 se determined from the two-state analysis of thermal unfolding data.

57 o-state model to far- and near-UV CD and DSC thermal unfolding data.

58 are assigned by inspection and used to study thermal unfolding equilibria over the entire transition

59 However, circular dichroism (CD) thermal unfolding experiments demonstrated that LC/A3 is

60 Thermal unfolding experiments indicate that a hydrophobi

61 Thermal unfolding experiments monitored by steady state

62 Thermal unfolding experiments monitored by ultraviolet s

63 e effects (KIEint) on the chemical step, and thermal unfolding experiments of both l- and h-DHFR show

64 nge the overall secondary structure, whereas thermal unfolding experiments revealed that fluorescent

65 Thermal unfolding experiments show that the complex has

66 Thermal unfolding experiments suggest that this process

67 Thermal unfolding experiments suggested that removal of

68 Urea denaturations and thermal unfolding experiments were used to measure the f

69 In vitro thermal unfolding experiments, as well as assessment of

70 elix bundle by -0.8 kcal/mol, evaluated from thermal unfolding experiments.

71 g structure is -1.2 kcal/mol, evaluated from thermal unfolding experiments.

72 tants were as stable as wild-type decorin in thermal unfolding experiments.

73 peptide coiled-coil trimer, as evaluated by thermal unfolding experiments.

74 tence of a thermodynamic intermediate of the thermal unfolding/folding process, termed III h, which i

75 erent environments on their structures, plus thermal unfolding/folding series.

76 Thermal unfolding followed by differential scanning calo

77 Aggregation accompanies thermal unfolding for both proteins under most condition

78 troscopy over a range of temperature through thermal unfolding has been applied to the low-spin, ferr

79 Thermal unfolding has been studied as a function of W0,

80 Some thorough studies of thermal unfolding have been carried out; however, protei

81 dynamical regime is attained in proximity of thermal unfolding in all solvents that we tested.

82 ly identical, indicating that stabilized MDH thermal unfolding intermediates are not determined by th

83 havior of human versus murine TNF-alpha upon thermal unfolding is due to differences in the solubilit

84 for both proteins under most conditions, but thermal unfolding is reversible and two-state for EP3 at

85 Thermal unfolding is stochastic, whereas mechanical unfo

86 luctuations of proteins exists, beyond which thermal unfolding is triggered.

87 Thermal unfolding measurements by circular dichroism ind

88 Equilibrium thermal unfolding measurements of the D-Arg peptide moni

89 his is the first report of a two-step, lasso thermal unfolding mechanism driven by the two steric con

90 rmatic methods allowed to get insight into a thermal unfolding mechanism.

91 Thermal unfolding ("melting") experiments are widely use

92 Thermal unfolding of 9+ ions of cytochrome c proceeds th

93 The midpoint of thermal unfolding of a 1 M protein solution (T degree va

94 The thermal unfolding of a 40-residue helix-turn-helix subdo

95 -cell NMR to follow at atomic resolution the thermal unfolding of a beta-barrel protein inside mammal

96 ifferent functional domains, we analyzed the thermal unfolding of a homopentameric LGIC, the 5-hydrox

97 We study the thermal unfolding of a protein, probed by two protein na

98 The thermal unfolding of a recombinant monoclonal antibody I

99 The thermal unfolding of a series of 6-, 10-, and 14-mer cyc

100 The enthalpy for thermal unfolding of AcWL(5) beta-sheets in the membrane

101 In addition, we analyze the thermal unfolding of alpha-t, IgE, and their complex.

102 ll as real experimental amide I' spectra for thermal unfolding of an alpha-helical peptide.

103 or coordination in MCO proteins, we assessed thermal unfolding of apo and metallated forms of Fet3p b

104 At protein concentrations <5 mg/ml, thermal unfolding of apoA-1 is resolved as an extended p

105 Here, we investigate the thermal unfolding of beta2S-PDZ at different pH and urea

106 The kinetics of thermal unfolding of both of these enzymes were consiste

107 Analysis of the chemical and thermal unfolding of both proteins indicates a value of

108 BI prevents thermal unfolding of Broccoli at 37 degrees C, leading t

109 We show here that thermal unfolding of BSF occurs in two distinct steps co

110 Thermal unfolding of discoidal complexes of apolipoprote

111 The thermodynamic parameters of the thermal unfolding of each of the labeled segments were o

112 s including the volume change resulting from thermal unfolding of each protein.

113 structure was also suggested by studying the thermal unfolding of g3p: the TolA binding site on D1, w

114 f N-linked carbohydrate increases the Tm for thermal unfolding of gOMCHI1 over rOMCHI1 by 2-4 degrees

115 In the virion the thermal unfolding of gp4 is prevented by the interaction

116 We reexamined the thermal unfolding of lipid-free apoA-1 in low-salt solut

117 The thermal unfolding of MW is reversible with a melting tem

118 The thermal unfolding of Nkx2.5(C56S) at pH 6.0 or 7.4 is a

119 CD spectroscopy measuring the thermal unfolding of NKX3.1 constructs showed a 2 degree

120 Thermal unfolding of PaaR2 is reversible and displays se

121 Thermal unfolding of PE, as evidenced by changes in alph

122 ay represent the first example of reversible thermal unfolding of peptides in membranes, help to defi

123 We studied the thermal unfolding of recombinant ERalpha by circular dic

124 We simulate the thermal unfolding of rhodopsin by breaking the native-st

125 Two mechanisms have been proposed for the thermal unfolding of ribonuclease S (RNase S).

126 ins within rNK1 was tested by monitoring the thermal unfolding of rNK1/K1 when in the presence of the

127 uctural features, at an atomic level, of the thermal unfolding of Ros87 and compared them to the beha

128 Thermal unfolding of the 5-hydroxytryptamine receptor oc

129 We here compare thermal unfolding of the apo and holo forms of Desulfovi

130 The best-fitted values for the thermal unfolding of the apo-protein were 60.9 +/- 0.2 d

131 Thermal unfolding of the C-terminal domain occurred over

132 The thermal unfolding of the cleaved ribozyme was also exami

133 tinct and independent transitions during the thermal unfolding of the enzyme.

134 protein-ligand complex (NL<==>N + L) and the thermal unfolding of the free protein (N<==>U).

135 these sites contribute to stability against thermal unfolding of the isolated VWF A2 domain.

136 Chemical and thermal unfolding of the L93A monomer followed by circul

137 Thermal unfolding of the monomeric subunits occurred wit

138 l scanning calorimetry (DSC) showed that the thermal unfolding of the myosin II rod is reversible and

139 Reversible thermal unfolding of the nonphosphorylated N-terminal do

140 We investigated the thermal unfolding of the peripheral subunit binding doma

141 In contrast, the thermal unfolding of the proposed major and highly stabl

142 Thermal unfolding of the T domain, measured by circular

143 for the high stability and reversibility of thermal unfolding of the triple variant.

144 Concurrently, thermal unfolding of these forms of EI has been monitore

145 Moreover, the thermal unfolding of these proteins does not result in t

146 The thermal unfolding of these proteins is > 90% reversible,

147 The guanidine-HCl induced unfolding and thermal unfolding of these proteins were studied to char

148 h alpha-helical proteins and the cooperative thermal unfolding of these proteins.

149 ism and infrared data also indicate that the thermal unfolding of these two monomeric coiled-coils ca

150 quilibrium IR measurements indicate that the thermal unfolding of this beta-hairpin is fairly broad.

151 We studied the thermal unfolding of ubiquitin in the presence of differ

152 nd transient conformational changes upon the thermal unfolding of ubiquitin were investigated with no

153 Thermal unfolding of variant apoA-I/DMPC complexes monit

154 molecular dynamics simulations and examined thermal unfolding of wild type and mutant SERT variants

155 cence-based dye binding assay to measure the thermal unfolding of wild-type (wt), DeltaLid, and S17D

156 With the exception of triple variant, thermal unfolding of wtFGF1 and the other variants is ir

157 The thermal unfolding of zwittergen-solubilized class 2 trim

158 Here, we surmised that thermal unfolding offered a glimpse on the folding energ

159 ough the protein does not exhibit reversible thermal unfolding, one can determine its stability param

160 l features at 37 degrees C, with significant thermal unfolding only occurring at temperatures >or=50

161 e thermal stability and the cooperativity of thermal unfolding over a wide pH range (0.9-7.5).

162 raction, the protein undergoes an intriguing thermal unfolding pattern which was studied based on a s

163 The site-specific thermal unfolding probed by (13)C isotopically edited IR

164 69 of the 75 interresidue sites, shows that thermal unfolding proceeds via a diversity of intermedia

165 The global thermal unfolding process and the partial or local struc

166 in CP thermal stability, we have probed the thermal unfolding process as a function of scan rate of

167 The thermal unfolding reactions are irreversible and, for th

168 Thermal unfolding reactions at pH 7.4 are reversible and

169 Surprisingly, thermal unfolding/refolding was found to be irreversible

170 (MBP-(85-99)) showed increased stability to thermal unfolding relative to the empty DR2-derived RTLs

171 explain these observations we used atomistic thermal unfolding simulations to identify ensembles whos

172 We in addition identified a thermal unfolding state with a structured core for all t

173 roscopy, analytical ultracentrifugation, and thermal unfolding studies indicate that the A and B pept

174 Thermal unfolding studies show that the CPmac mutations

175 Urea denaturation and thermal unfolding studies show that the N136A mutant enz

176 A thermal unfolding study of the 45-residue alpha-helical

177 at pH 3.5, with a > or = 20 degrees C higher thermal unfolding temperature at all pHs.

178 The midpoint thermal unfolding temperature of the fluorinated peptide

179 gimes whose breakpoint occurs near the first thermal unfolding temperature of the mAb domain structur

180 CR decreased the apparent thermal unfolding temperature of the protein.

181 observed little ionic strength effect on the thermal unfolding temperature, Tm, values in these syste

182 revealed that A2-VicCC was more resistant to thermal unfolding than A2-DeltaCC.

183 e sensitive to acid-induced denaturation and thermal unfolding than sbR(WT).

184 sensitive fluorescent dye to monitor protein thermal unfolding, the ligand-binding affinity can be as

185 SD) matrix of the conformations sampled in a thermal unfolding trajectory, have also been used to ide

186 in embedded in different environments as the thermal unfolding transition is approached.

187 However, the cooperativity of the thermal unfolding transition is reduced by the presence

188 dominated by alpha-helices and a cooperative thermal unfolding transition of 49 degrees C, features c

189 es from that of the holoenzyme: (1) the main thermal unfolding transition of the apo-HCA II is lowere

190 The thermal unfolding transition temperature (T(m)) of rhodo

191 The thermal unfolding transition temperature of this protein

192 label was exploited to probe the equilibrium thermal unfolding transition using temperature-dependent

193 beta-sheet undergoes a broad but cooperative thermal unfolding transition with a midpoint at approxim

194 this domain, psbd41, undergoes a cooperative thermal unfolding transition with a tm of 54 degrees C.

195 ding a tendency to aggregate, noncooperative thermal unfolding transition, and a loosely packed core.

196 e proteins exhibited a cooperative two-state thermal unfolding transition, and DR2-derived RTLs with

197 000 s-1 near the midpoint of the equilibrium thermal unfolding transition.

198 ed it to be folded with a weakly cooperative thermal unfolding transition.

199 ure with a reversible and highly cooperative thermal unfolding transition.

200 ry (DSC), several laboratories find that the thermal unfolding transitions of alpha alpha and beta be

201 Consistent with this data, the shape of thermal unfolding transitions varies from asymmetric for

202 ees C) at the approximate midpoints of their thermal unfolding transitions were found to be similar.

203 resonance spectroscopy, we demonstrate that thermal unfolding under amyloidogenic solution condition

204 Thermal unfolding was further studied in the presence of

205 The T(m) for thermal unfolding was inversely related to acyl chain pa

206 To probe the transition state of ubiquitin thermal unfolding, we examine unfolding dynamics and kin

207 The energetics of thermal unfolding were also evaluated by differential sc

208 Conformational dynamics during thermal unfolding were probed by hydrogen/deuterium (H/D

209 ncomplete, cooperative, partially reversible thermal unfolding with increased unfolding temperature a

210 the protein undergoes reversible, two-state thermal unfolding with Tm = 70.0 +/- 0.3 degrees C and a