ライフサイエンスコーパス: thiolase

1 ogen biosynthesis and peroxisomal 3-ketoacyl thiolase.

2 shed by deleting the first 16 amino acids of thiolase.

3 ent of the peroxisomal matrix, e-oxoacyl-CoA thiolase.

4 ted whether Peb1p interacts with the PTS2 of thiolase.

5 RL3_01526 (kctA) encodes beta-ketoadipyl-CoA thiolase.

6 and reduction of p46Shc expression activates thiolase.

7 ifunctional enzyme, and 3-oxoacyl-coenzyme A thiolase.

8 -CoA hydratase and long-chain 3-ketoacyl-CoA thiolase.

9 ith the mango, cucumber, and rat peroxisomal thiolases.

10 separate clades and are distantly related to thiolases.

11 thylglutaryl-CoA synthases, and biosynthetic thiolases.

12 with amino acid sequence homology to type II thiolases.

13 2 acx2-1 double mutants and the ketoacyl-CoA thiolase-2 (kat2) mutant exhibit a sucrose-independent g

14 l coenzyme A (acetyl-CoA) acetyltransferase (thiolase), 3-hydroxybutyryl-CoA dehydrogenase, and acyl-

15 te could be partially restored by expressing thiolase (a PTS2-containing enzyme) fused to the PTS1.

16 studies showed colocalization of HsPMP20 and thiolase, a bona fide peroxisomal protein.

17 Acetoacetyl CoA thiolase (AACT, EC 2.3.1.9) catalyzes the condensation o

18 is the lipid oxidation enzyme 3-ketoacylCoA thiolase ACAA2, to which p46Shc binds directly and with

19 -relevant p46Shc, an endogenous inhibitor of thiolase, actions that are expected to suppress MKP.

20 The thiolase active site is also characterized by two oxyani

21 g the cysteine, considered to be part of the thiolase active site, the kiwifruit protein shows approx

22 AcAc-CoA thiolase activities also paralleled HMG-CoA reductase an

23 cytosolic acetoacetyl coenzyme A (AcAc-CoA) thiolase activities.

24 hromatography was shown to have both ACT and thiolase activities.

25 y to regulatory stimuli than acetoacetyl-CoA thiolase activity but usually less than HMG-CoA reductas

26 es its expression and eliminates most of the thiolase activity in seedlings.

27 nificantly lower (k(cat) 3 min(-1)) than the thiolase activity of FadA (k(cat) 2170 min(-1)).

28 enzymes indicated that viperin inhibits the thiolase activity of HADHB, but, unexpectedly, HADHB act

29 uggest p46Shc to be a negative mitochondrial thiolase activity regulator, and reduction of p46Shc exp

30 localizing viperin to mitochondria decreased thiolase activity, and coexpression of HADHB significant

31 on of dominant negative p46Shc reduced ACAA2 thiolase activity, improved beta-oxidation, and reduced

32 f a protein that directly binds and controls thiolase activity.

33 gher lipid oxidation capacity, and increased thiolase activity.

34 ocated downstream from yfcY, a gene encoding thiolase activity.

35 greater than 99% decrease in k(cat) for the thiolase activity.

36 indicating that the mutant lacks long-chain thiolase activity.

37 Mature thiolase alone, lacking the PTS2 signal, was not importe

38 sely affected the activity of 3-ketoacyl-CoA thiolase although not enough to become rate-limiting.

39 structural homology to aldolases, acts as a thiolase, an activity previously undescribed for this fa

40 e mevalonate pathway, acetoacetyl-coenzyme A thiolase and 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-

41 Similarly, induction of hepatic thiolase and bifunctional enzyme also required expressio

42 e product catalyzed both the acetoacetyl-CoA thiolase and HMG-CoA reductase reactions.

43 The catalytic properties of 3-ketoacyl-CoA thiolase and l-3-hydroxyacyl-CoA dehydrogenase of the mu

44 Under conditions at which acetoacetyl-CoA thiolase and long-chain thiolase were completely inactiv

45 ion, belonging to two protein superfamilies: thiolases and acyl-CoA N-acyltransferases.

46 ith ACP has not previously been observed for thiolases and in the case of the S. collinus FadA is sig

47 ort (n = 208) found three of these microbial thiolases and one acyl-CoA N-acyltransferase to be epide

48 erved as the nucleophile and general base in thiolases and replaced a pair of oxyanion-hole histidine

49 roxisomes or for the import of 3-oxoacyl-CoA thiolase (and at least two other peroxisomal matrix prot

50 cyl-CoA oxidase, L-bifunctional protein, and thiolase, and (b) a second noninducible pathway catalyzi

51 Moreover, the stabilized ICL, thiolase, and an ICL-GFP reporter remained peroxisome as

52 tor-activated receptor alpha (PPARalpha) and thiolase, and an up-regulation of PPARgamma, a positive

53 hase, acyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, and many proteins involved in chaperone activi

54 At this stage, the basal mRNA levels of HD, thiolase, and other peroxisome proliferator-induced targ

55 me A (CoA), acyl CoA synthetase, oxoacyl CoA thiolase, and ubiquitin also were underexpressed in NASH

56 drial aldehyde dehydrogenase, 3-ketoacyl-CoA thiolases, and adenosine triphosphate synthase.

57 e and reduced accumulation of 3-ketoacyl-CoA thiolase, another PTS2-containing protein; both defects

58 e 2 peroxisomal targeting sequence (PTS2) of thiolase are defective, whereas the biogenesis of protei

59 Thiolases are CoA-dependent enzymes that catalyze the th

60 Thiolases are dimers or tetramers (dimers of dimers).

61 n chains and a coenzyme moiety-unusual for a thiolase-are unknown.

62 lso related to the E. coli and mitochondrial thiolases, as well as to the acetoacetyl-CoA thiolases o

63 In the thiolase assay kinetic analyses revealed similar K(m) va

64 oxisomal Arabidopsis thaliana 3-ketoacyl-CoA thiolase (AtKAT), an enzyme of fatty acid beta-oxidation

65 Our results expand the utility of thiolase-based pathways and provide biosynthetic access

66 thiolase, which is a bacterial biosynthetic thiolase belonging to the CT-thiolase subfamily.

67 PTS2 protein imports increased the level of thiolase bound and imported into the organelles.

68 Although the basics of thiolase chemistry are precedented, the mechanism by whi

69 nase deficiency, medium-chain 3-ketoacyl-CoA thiolase deficiency, 3-hydroxy-3-methylglutaryl-CoA synt

70 In competition experiments, mature thiolase did not affect the import of a PTS1 protein, bu

71 The import of the PTS2 protein thiolase differed from PTS1 protein import in several wa

72 Thiolase differs from these other peroxisomal proteins i

73 onate is virtually nil because acetoacyl-CoA thiolase does not favor the formation of beta-ketopentan

74 mature protein competes with the full-length thiolase during assembly of an import complex at the sur

75 t was missing the peroxisomal 3-ketoacyl-CoA thiolase encoded by the PEROXISOME DEFECTIVE1 (PED1/At2g

76 in ciliated neurons and by a 3-ketoacyl-coA thiolase (encoded by kat-1) that acts in fat storage tis

77 The catalytic properties of 3-ketoacyl-CoA thiolase, enoyl-CoA hydratase, and delta 3-cis-delta 2-t

78 In this regard, synthetic pathways based on thiolase enzymes to catalyze the initial carbon-carbon b

79 the previously characterized CoA-ligase and thiolase enzymes, provides evidence that the whole pathw

80 Using a new family of iterative thiolase enzymes, we genetically engineered a microbial

81 Our discovery of a highly active thiolase establishes an alternative enzymatic route to p

82 Features distinguish it from members of the thiolase family, suggesting that it carries out a relate

83 synthetic enzymes, acetoacetyl-CoA thiolase (thiolase), farnesyl diphosphate phosphatase, and farnesa

84 , like other KAS-II enzymes, mtKasB adopts a thiolase fold but contains unique structural features in

85 om Enterococcus is a member of the family of thiolase fold enzymes and, while similar to the recently

86 leaves, as does PED1 (encoding a 3-keto-acyl-thiolase for beta-oxidation).

87 n fluorescent protein chimera of peroxisomal thiolase, formation of peroxisomes, and peroxisome funct

88 oleate-induced PTS2-dependent import of the thiolase Fox3p into peroxisomes is conducted by the solu

89 SNO-CoA reductase, protects acetoacetyl-CoA thiolase from inhibitory S-nitrosylation and thereby aff

90 ivity is consistent with a beta-ketoacyl-CoA thiolase function in cholesterol beta-oxidation that is

91 CtSal, suggesting an aldolase rather than a thiolase function.

92 ndogenous clostridial promoters: that of the thiolase gene (thlP) and that for the clostridial transc

93 -acetyl CoA, and KAT2 appears to be the only thiolase gene expressed at significant levels during ger

94 t loss-of-function mutations of the ketoacyl thiolase gene kat-1 result in an increased accumulation

95 An inhibitor of long-chain 3-ketoacyl-CoA thiolase has been developed as a tool for probing the co

96 se/3-hydroxyacyl-CoA dehydrogenase (HD), and thiolase, has been examined in mice by disrupting ACOX g

97 All thiolases have two reactive cysteines: (a) a nucleophili

98 y using HMG-CoA synthase and acetoacetyl-CoA thiolase/HMG-CoA reductase from E. faecalis.

99 Ltp2 adopted a fold similar to those in thiolases; however, instead of forming a deep tunnel, th

100 n CNS-associated macrophages and peroxisomal thiolase immunostaining after cuprizone exposure was inc

101 Kinetic studies of acetoacetyl-CoA thiolase implicated a ping-pong mechanism.

102 Thiolase import was slower than typical PTS1 protein imp

103 irming that the PTS2 signal is necessary for thiolase import.

104 sing p46Shc stimulates enzymatic activity of thiolase in vitro Thus, we suggest p46Shc to be a negati

105 Pex7p is functional, resulting in import of thiolase into peroxisomes and improved growth of the yea

106 at is selectively defective in the import of thiolase into peroxisomes but has a normal ability to pa

107 The activity of 3-ketoacyl-CoA thiolase involved in mitochondrial beta-oxidation of fat

108 Peb1p is found in peroxisomes whether thiolase is expressed or not.

109 t the peroxisomal packaging of PTS2 targeted thiolase is lacking.

110 Thiolase is the last enzyme of the mitochondrial fatty a

111 The best characterized thiolase is the Zoogloea ramigera thiolase, which is a b

112 The subunit, a typical thiolase, is a combination of two similar alpha/beta dom

113 mosome 2 (KAT2), which encodes a peroxisomal thiolase, is activated in early seedling growth.

114 3-ketoacyl-CoA thiolase (KAT) (EC: 2.3.1.16) catalyses a key step in fa

115 Reduced fertilization was also observed in thiolase (kat2-1) and peroxisomal acyl-Coenzyme A synthe

116 Of the two major 3-ketoacyl coenzyme A thiolases, KAT2 plays the primary role in BA synthesis.

117 cation and biochemical characterization of a thiolase-ketoreductase pair involved in production of br

118 The fusion protein was expressed in a thiolase knockout strain.

119 id residues were fused in front of truncated thiolase lacking the NH2-terminal 16-amino acid PTS2.

120 lectively inhibits long-chain 3-ketoacyl CoA thiolase (LC 3-KAT), thereby reducing fatty acid oxidati

121 a Trypanosoma brucei homolog, annotated as a thiolase-like protein (TbSLP), shares the catalytic arch

122 thiolases, as well as to the acetoacetyl-CoA thiolases of prokaryotes.

123 ive Claisen condensation by polyketoacyl-CoA thiolases offers a more efficient alternative.

124 The thiolase-p46Shc connection shown here in vitro and in or

125 Finally, a thiolase-Peb1p complex was isolated by immunoprecipitati

126 ies of the mutant strains indicated that the thiolase reaction is the limiting step in PHB biosynthes

127 Recent insights concerning the thiolase reaction mechanism, as obtained from recent str

128 were completely inactivated, 3-ketoacyl-CoA thiolase retained some activity.

129 lity of residues within the motif, rat liver thiolase (rthio) and various chimeric chloramphenicol ac

130 Three thiolase subfamilies can be identified, each characteriz

131 al biosynthetic thiolase belonging to the CT-thiolase subfamily.

132 carrier protein synthases are members of the thiolase superfamily and are key regulators of bacterial

133 Degradative thiolases, which are part of the thiolase superfamily and naturally function in the beta-

134 that is critical for this process is OleA, a thiolase superfamily enzyme that condenses two fatty acy

135 OleA is a thiolase superfamily enzyme that has been shown to catal

136 In thiolase superfamily enzymes, catalysis is achieved via

137 udies of the HMG-CoA synthase members of the thiolase superfamily have shown that the catalytic loop

138 OleA is the first characterized thiolase superfamily member that has two long-chain alky

139 rmation can be catalysed by enzymes from the thiolase superfamily, including beta-ketoacyl-acyl-carri

140 etabeta fold, which is characteristic of the thiolase superfamily.

141 substrates of this particular member of the thiolase superfamily.

142 sidue five, a change that is known to reduce thiolase targeting in vivo.

143 ha-branched products even when paired with a thiolase that highly prefers unbranched linear products.

144 erved a strong interaction between Peb1p and thiolase that was abolished by deleting the first 16 ami

145 rease the amount of radiolabeled full-length thiolase that was imported.

146 546) gene, annotated as a lipid-metabolizing thiolase, the expression of which is upregulated by chol

147 ed genes including peroxisomal 3-oxoacyl-CoA thiolase (THIO), peroxisomal enoyl-CoA hydratase/3-hydro

148 ing JH biosynthetic enzymes, acetoacetyl-CoA thiolase (thiolase), farnesyl diphosphate phosphatase, a

149 ofluorescence, localization of 3-oxoacyl-CoA thiolase to peroxisomes was unchanged whether Pal1 was p

150 ctively to increased and decreased levels of thiolase transcripts, which is one of the early genes of

151 Thiolases typically utilize a ping-pong mechanism center

152 ld lower and the Km for the substrate of the thiolase was 6-fold higher.

153 de consisting of the first 16 amino acids of thiolase was sufficient for the affinity binding of Peb1

154 Thiolase was thought previously only to be regulated by

155 hich acetoacetyl-CoA thiolase and long-chain thiolase were completely inactivated, 3-ketoacyl-CoA thi

156 ICL and thiolase were mislocalized to the cytosol but only ICL w

157 ICL, MLS, and the beta-oxidation enzyme thiolase were stabilized in the pex4-1 pex22-1 double mu

158 Of all beta-oxidation enzymes, only thiolases were inactivated by the inhibitor.

159 on enzymes (CYP4A3, bifunctional enzyme, and thiolase) were observed in the livers of HNF1alpha-null

160 dehydrogenase, and long-chain 3-ketoacyl-CoA thiolase, were determined with substrates having acyl ch

161 ty acyl-CoA oxidase, bifunctional enzyme, or thiolase, which accompanies peroxisome proliferation in

162 racterized thiolase is the Zoogloea ramigera thiolase, which is a bacterial biosynthetic thiolase bel

163 Degradative thiolases, which are part of the thiolase superfamily an

164 related to the peroxisomal beta-ketoacyl-CoA thiolases, which catalyze the CoA-dependent degradative

165 e from Cupriavidus necator, we identify five thiolases with TAL production activity.