ライフサイエンスコーパス: tissue culture

1 y sequencing when the virus is propagated in tissue culture.

2 expression resulting in enhanced shooting in tissue culture.

3 pergillus fumigatus and evaluated ex vivo in tissue culture.

4 onocytogenes invasion of epithelial cells in tissue culture.

5 as historically been refractory to growth in tissue culture.

6 rical (Mayinga) EBOV strains was observed in tissue culture.

7 r and to prevent the replication of HIV-1 in tissue culture.

8 ood culture bottle system for periprosthetic tissue culture.

9 antagonizing replication of some viruses in tissue culture.

10 embryogenesis or experimentally in in vitro tissue culture.

11 C, were further characterized against HCV in tissue culture.

12 of recent human H3N2 viruses upon passage in tissue culture.

13 zed transfection of C. parvum sporozoites in tissue culture.

14 28 aids in promoting experimental latency in tissue culture.

15 luorene 11 as a potent DNA cleavage agent in tissue culture.

16 d not convert back to tachyzoites readily in tissue culture.

17 gh these two cell lines migrate similarly on tissue culture.

18 C results in a slow propagation phenotype in tissue culture.

19 sitivity compared to standard periprosthetic tissue culture.

20 the same organism were considered a positive tissue culture.

21 venting the outgrowth of aneuploid clones in tissue culture.

22 ll-cell adhesion, and formation of crypts in tissue cultures.

23 mix-ups and contamination in human cell and tissue cultures.

24 thelial cell lines were grown in vivo versus tissue cultures.

25 rodes to deliver alternating EFs to cell and tissue cultures.

26 ture, along with conventional periprosthetic tissue cultures.

27 nce that the spontaneous mutation T544I is a tissue culture adaptation in certain cell lines and that

28 Tissue culture adaptation of cytomegaloviruses rapidly s

29 a single passage, consistent with it being a tissue culture adaptation.

30 the interaction between alphavbeta6 and two tissue culture adapted FMDV strains by cryo-electron mic

31 Infection can also occur in tissue culture adapted virus in the absence of integrin

32 d to the wild-type (WT) PoSaV Cowden strain, tissue culture-adapted (TC) PoSaV has two conserved amin

33 highly virulent U.S. PEDV strain PC21A, the tissue culture-adapted PC177 (TC-PC177) contains a 197-a

34 The tissue culture-adapted porcine sapovirus Cowden strain i

35 n gnotobiotic pigs than those induced by the tissue culture-adapted strain.

36 BTV serotypes are incorporated into a common tissue-culture-adapted backbone.

37 exogenous fluorescent reporter gene in both tissue culture and a live animal model, as well as light

38 The phenotypic (determined via tissue culture and a vaginal colonization model) and reg

39 In tissue culture and animal models, sex hormones regulate

40 gap filling conferred therapy resistance in tissue culture and BRCA patient tumors.

41 at lyses single adherent cells from standard tissue culture and captures the contents to perform sing

42 A in trans rescued KLKI MHV68 replication in tissue culture and enabled detection of KLKI MHV68 react

43 bility than CD24(-)CD29(-)/CD49f(-) cells in tissue culture and enhanced metastatic potential in allo

44 gy pathway caused more rapid viral spread in tissue culture and greater pathogenicity in mice.

45 G-to-A mutations in the HIV genome, both in tissue culture and in HIV positive patients undergoing K

46 ing a recombinant virus-expression system in tissue culture and in mice, we demonstrate that gentamic

47 e resulting mutant viruses were evaluated in tissue culture and in mice.

48 Both in tissue culture and in mouse models, these imaging biocon

49 In tissue culture and in the liver colonies derived from ca

50 Combined tissue culture and in vivo data support that environment

51 Both tissue culture and in vivo experiments in mice demonstra

52 ion was examined by using human keratinocyte tissue culture and mouse models.

53 ifested as a decrease in infectivity in both tissue culture and murine infection models.

54 ll, of the capsid protein is rapidly shed in tissue culture and primary target cells, independent of

55 gene-edited meristems sidesteps the need for tissue culture and promises to overcome a bottleneck in

56 lacking a rigid cell wall and is observed in tissue culture and single-celled organisms, as well as i

57 We report our experience with periprosthetic tissue culture and sonication culture of antimicrobial a

58 ctors through two-photon excitation (2PE) in tissue culture and whole-animal studies.

59 go by passaging wild-type JEV strain SA14 in tissue cultures and rodents, with intermittent tissue cu

60 molecular approaches were used together with tissue-culture and mouse models to conduct phenotypic co

61 e peptide cleavage sites also varied between tissue-cultured and primary cells, with 5- and 8-residue

62 Once thought of as a tissue culture artefact by some researchers, senescence

63 t not SARS-CoV, as measured by two different tissue culture assays, as well as a replication-competen

64 In addition, in tissue culture, at concentrations higher than anthelmint

65 Finally, in vitro tissue culture-based analyses implicate the ompA RNA the

66 ged revisions, as detected by periprosthetic tissue cultures; both have low sensitivities to detect p

67 a derivative strain passaged extensively in tissue culture (BTV8H) in in vitro and in vivo studies.

68 netics similar to that of wild-type virus in tissue culture but caused a dramatically more aggressive

69 Virus growth in tissue culture can be attenuated by introduction of muta

70 cid-derived ammonia, most mammalian cells in tissue culture cannot proliferate or even survive in an

71 embryonic brain tissue, as well as in human tissue culture cell lines using RNA in situ hybridizatio

72 eltaSHDeltaV indicated that it was stable in tissue culture cell lines.

73 cellular distributions when overexpressed in tissue culture cells (HEK293), and can induce the format

74 Tissue culture cells and Caenorhabditis elegans embryos

75 We show that 'EC-tagging' occurs in tissue culture cells and Drosophila engineered to expres

76 inase A (PKA) activity stabilizes PER, in S2 tissue culture cells and in fly circadian neurons.

77 ted in quiescent cells including G0-arrested tissue culture cells and prophase I-arrested oocytes.

78 thodology to measure microtubule assembly in tissue culture cells and Xenopus egg extracts using two-

79 spindle assembly, we combined experiments in tissue culture cells and Xenopus laevis egg extracts wit

80 Similar rings are observed in Xenopus tissue culture cells at a lower frequency but are enrich

81 ways compromises bipolar spindle assembly in tissue culture cells but not in X. laevis egg extracts.

82 Human tissue culture cells have long been a staple of molecula

83 glycoproteins and protected mucus-producing tissue culture cells in vitro.

84 luster causes altered IL-6 induction both in tissue culture cells induced by LPS treatment in vitro a

85 outine procedure used to dissociate adherent tissue culture cells prior to RNA extraction.

86 In vitro, depletion of FL2 from mammalian tissue culture cells results in a more than twofold incr

87 However, our experiments in tissue culture cells show that viral RNA synthesis and n

88 filamentous structures, or rodlets, in human tissue culture cells, after gene transfer to adult mice,

89 vivo, both in Xenopus embryos and mammalian tissue culture cells, also impacts nuclear size.

90 y(A) tail shortening and mRNA degradation in tissue culture cells, and we detect a reduced number of

91 Therefore, in tissue culture cells, input and progeny genomes are not

92 Using human tissue culture cells, we found that hEndoU localizes to

93 Using semi-intact tissue culture cells, we performed a polysome solubiliza

94 croscopy (STORM) to Xenopus egg extracts and tissue culture cells, we report various distribution pat

95 d exchange at the frequency of 5% in primary tissue culture cells, whereas higher levels were seen in

96 ogenous C-terminal gene tagging in mammalian tissue culture cells.

97 tore the mutant's ability to form plaques in tissue culture cells.

98 tor), which inhibits several HECT ligases in tissue culture cells.

99 bserved enhancer activity of this element in tissue culture cells.

100 hen ectopically expressed in jump muscles or tissue culture cells.

101 swarming motility, and form small plaques in tissue culture cells.

102 ogical and biochemical consequences in human tissue culture cells.

103 n autonomously in single-cell eukaryotes and tissue culture cells; however, within the context of a m

104 ntry and that of infectious EBOV and MARV in tissue cultured cells.

105 n activator Chorion factor 2 in flies and in tissue-culture cells.

106 thereby negatively regulate YAP1 activity in tissue-cultured cells.

107 an be overcome by adaptation of the virus in tissue-cultured cells.

108 e have been able to overcome these blocks in tissue-cultured cells.

109 orcine TMJ discs were measured under varying tissue culture conditions (n = 40 discs), and mechanical

110 luated in regenerants derived under distinct tissue culture conditions and analysed via Taguchi stati

111 can be precisely controlled in standard cell/tissue culture conditions and in vivo.

112 This creates an increasing need for tissue culture conditions better adapted to live-cell im

113 We find that under standard serum-containing tissue culture conditions, ATSP-7041 achieves intracellu

114 f a spontaneous mutation (T544I) specific to tissue culture conditions, suggesting that it has no rol

115 rosis compared to cells cultured in standard tissue culture conditions.

116 strain with no discernible growth defect in tissue culture, contained a 2,158-bp deletion in open re

117 oss of Karma methylation and of small RNA in tissue culture contributes to the origin of mantled, whi

118 lation of infectious virus on the basis of a tissue culture cytopathic effect, an increase in virus g

119 plate-reader enzyme immunoassay, and direct tissue culture cytotoxicity.

120 The structure and dynamics of tissue cultures depend strongly on the physical and chem

121 These results indicate that tissue culture, depending on the protocol used, can indu

122 immunization of the submandibular gland with tissue culture-derived murine cytomegalovirus (tcMCMV) o

123 In tissue culture, DLL1 induced proliferation of human peri

124 Transfer to a tissue culture environment resulted in rapid and extensi

125 cells in vitro and in three-dimensional lung tissue cultures ex vivo.

126 togenic components in three-dimensional lung tissue cultures ex vivo.

127 ion of this glycoproteomic platform to human tissues cultured ex vivo.

128 in situ hybridization as well as ex vivo IPF tissue culture experiments were done using healthy donor

129 Mammalian tissue-culture experiments suggest that downstream of MS

130 he high concentrations of ivermectin used in tissue-culture experiments.

131 Pulldown assays from Arabidopsis thaliana tissue culture extracts with the mitochondrial MORF1 and

132 have a small effect on virus replication in tissue culture, few studies have confirmed this mechanis

133 rage-dependent cells (OV-90AD) were grown in tissue culture flasks, whereas anchorage-independent cel

134 NA prepared from 10-fold serial dilutions of tissue culture fluid containing DENVs suggested that the

135 /cm pk-pk) electric fields (EFs) to cell and tissue cultures generated using induced electromagnetic

136 ted transformation through the generation of tissue culture had limited success for Setaria viridis,

137 e VZV genome when the virus is propagated in tissue culture.IMPORTANCE Viruses isolated from clinical

138 DMS that would, in turn, hamper cells and/or tissues cultured in the devices.

139 er time, mutations accumulate more slowly in tissue culture, in part because of the inefficiency of r

140 g of up to ten organ chips inside a standard tissue-culture incubator.

141 ransformation in addition to those caused by tissue culture-induced somaclonal variation.

142 The easiest way to manipulate total tissue culture-induced variation (TTCIV) is to utilise a

143 approach could be used for quantification of tissue culture-induced variation and provided direct evi

144 study describes the optimisation of in vitro tissue culture-induced variation in plant regenerants de

145 lymorphism analysis, was used to investigate tissue culture-induced variation in triticale regenerant

146 genetic background of donor plants affected tissue culture-induced variation.

147 erants towards maximum and minimum levels of tissue culture-induced variation.

148 didate vaccine (low dose [1.5 x 10(4) median tissue culture infection doses (TCID50) per 0.05 mL], me

149 In a tissue culture infection model, pretreatment of C. turic

150 ment and pathogen-mediated cytotoxicity in a tissue culture infection model.

151 The 1000 median tissue culture infectious dose (TCID50) dose produced mo

152 An optimal dose of 10(7) tissue culture infectious dose 50 was reached that cause

153 se-dependent reduction (0.7 to 1.5 log10 50% tissue culture infectious dose [TCID50]) in nasal wash v

154 reached similar mean yields of 3 x 10(7) 50% tissue culture infectious dose by 72 h infection in Huh-

155 amuscular injection at a dose of 1 x 109 50% tissue culture infectious dose on treatment days 22, 36,

156 Approximately 7 log(10) tissue culture infectious dose(50) (TCID(50)) of EBOV/Ma

157 om Yunnan province) at 10(4.5) TCID(50) (50% tissue culture infectious dose) per pig.

158 on was performed from 104 to 107 TCID50 (50% tissue culture infectious dose).

159 EBOV-Kikwit, between 4 and 27 times the 50% tissue culture infectious dose, were sufficient to cause

160 Median tissue culture infectious dose/mL was 1780 (interquartil

161 ecipients was 3.54, 2.77, and 3.72 log10 50% tissue culture infectious doses (TCID50)/mL, respectivel

162 DBA/2 mice were inoculated with 10(5) 50% tissue culture infective dose (TCID(50)) of D/bovine/Fra

163 of the assay was determined to be 0.004 50% tissue culture infective dose (TCID(50))/ml using inacti

164 BOV dilution from 4 x 10(7) to 4 x 10(2) 50% tissue culture infective dose (TCID50)/ml, as well as th

165 No surviving virus was revealed by a 50% tissue culture infective dose assay after the combined t

166 t serum (EBOV concentration of 1 x 10(8) 50% tissue culture infective dose per milliliter [TCID50 . m

167 particles) or MVA-BN-Filo (1 x 10(8) median tissue culture infective dose) and boosted with the alte

168 LD50) was determined to be 0.015 50% TCID50 (tissue culture infective dose) of MARV/Ang-MA in SCID mi

169 eductions in viral shedding, based on median tissue culture infective dose, were observed in patients

170 Doses ranging from 10(7) to 10(9) 50% tissue culture infective doses (TCID50) consistently inf

171 a 50% lethal dose (LD50) of 5.3 x 10(-2) 50% tissue culture infective doses (TCID50), was developed.

172 ic range of 8 log units and 1.3 x 10(-3) 50% tissue culture infective doses (TCID50)/ml of cultured M

173 sages ranged from 6 x 10(2) to 2 x 10(5) 50% tissue culture infective doses (TCID50)/ml.

174 ferrets intranasally at a dose of 10(6) 50% tissue culture infective doses in a range of inoculum vo

175 s ( approximately 10(6) genome copies or 50% tissue culture infective doses/ml).

176 one to reconcile conflicting observations in tissue culture, intact isolated ganglia and living anima

177 dification methods require regeneration from tissue culture, involving complicated, long and laboriou

178 the loss of mitogenicity seen previously in tissue culture is also seen in vivo, underscoring the po

179 However, plant tissue culture is known to engender frequent unwanted va

180 The creation of edited plants through tissue culture is often inefficient, time-consuming, wor

181 lternative model for early drug development, tissue culture, lacks both the architecture and, usually

182 Using a tissue culture M cell model, we examined S Typhi strains

183 We found that in tissue culture, mature, active, and nuclear forms of the

184 only are the levels of glutamine in standard tissue culture media at least ten-fold higher than other

185 12 mouse myotubes were incubated in standard tissue culture media, or media supplemented with 28 mM g

186 The tissues were preserved for 7 days in tissue culture medium at 31 degrees C.

187 Removal of glucose from tissue culture medium further enhanced these phenotypes

188 broth (MHB) and a 64-fold lower MIC in this tissue culture medium with 20% human serum.

189 ratory media, AZM had a 16-fold lower MIC in tissue culture medium with 5% Mueller Hinton broth (MHB)

190 also differ in stability at 37 degrees C in tissue culture medium.

191 aid in a sterile basin partially filled with tissue culture medium.

192 ith 1 mL mounted syringe was filled with the tissue culture medium.

193 Costly, genotype-dependent tissue culture methods are used in many crops, while see

194 table marker gene-selection combinations and tissue culture methods for efficient regeneration of tra

195 Plant tissue culture methods need optimization and simplificat

196 influenza virus replication in a human lung tissue culture model and observed strong inhibition of v

197 anges of signature miRNAs were examined in a tissue culture model of HCV in hepatoma cells: HCV infec

198 5 genetic variants in HEK 293 cells and in a tissue culture model of HCV infection revealed that the

199 e, we developed and characterized an ex vivo tissue culture model preserving these properties.

200 We have developed a tissue culture model to study the epigenetic basis for l

201 A tissue culture model was developed showing that a subset

202 3D tissue culture models are utilized to study breast cance

203 Whereas studies in 2-dimensional (2D) tissue culture models have suggested that vinculin negat

204 Standard tissue culture models lack critical emergent properties

205 efforts in epigenome editing were limited to tissue culture models of disease.

206 ein has multiple immunomodulatory effects in tissue culture models of infection, including NF-kappaB

207 eactivation, and we show here that indeed in tissue culture models this cellular transcription factor

208 xperimental challenge of human skin in vivo, tissue culture models, integration of "omics" data sets,

209 In tissue culture models, MaR1 treatment of bone marrow-der

210 hotgun mass spectrometry analysis founded on tissue culture models, we identified a candidate SIRT2 d

211 By combining in vivo and tissue culture models, we show here that VEGF165-induced

212 restriction on PCO using human lens cell and tissue culture models.

213 functional impact of physical symmetry in 3D tissue culture models.

214 ons and is more similar to that of classical tissue culture models.

215 essing the relevance of observations made in tissue culture models.

216 ructure in APPswe/PS1E9 transgenic mouse and tissue culture models.

217 cation, following bile duct ligation, and in tissue culture models.

218 In fact, reducing levels of SF3B2 in tissue-cultured neurons was effective against neurotoxic

219 Recent advances in tissue culture now enable the cultivation of human intes

220 Myogenesis is recapitulated in the tissue culture of myoblasts that differentiate by fusion

221 However, tissue cultures of cardiomyocyte monolayers currently re

222 xperiment is carried out in model organisms, tissue culture, or in vitro; typically addresses only a

223 models including two- and three-dimensional tissue cultures, organoids, genetically engineered mice

224 ed vaccines (chicken embryo origin [CEO] and tissue culture origin [TCO]), although effective, can re

225 Three-dimensional organotypic tissue culture (OTC) is an innovative three-dimensional

226 asis, but has not been studied using dynamic tissue culture paradigms.

227 a deletion that arose during the process of tissue culture passage can be repaired, with subsequent

228 44I mutation in the Makona GP1,2 gene during tissue culture passage in certain cell lines.

229 set of secondary adaptation mutations after tissue culture passage.

230 ssue cultures and rodents, with intermittent tissue culture plaque purifications, to produce a virus

231 Compared to islets cultured on tissue culture plastic (TCP), islets cultured on 3D-ECM

232 oncede that standard cell culture materials (tissue culture plastic and glass) do a poor job of recap

233 oth, micro, and nano) and a control surface (tissue culture plastic) with or without osteogenic suppl

234 PDL cells were plated for various times on tissue culture plastic, PDL-derived ECMs, collagen Type

235 ng-term propagation of hPS cells on uncoated tissue culture plastic.

236 soft environments or in them rather than on tissue-culture plastic better recapitulates the acinar m

237 ptides were synthesized and used to pre-coat tissue culture plates before lung derived ASM cells and

238 uential delivery of the mixed solutions into tissue culture plates is actuated by a novel mechanism b

239 as NIH3T3 cells that express it form foci in tissue culture plates, colonies in soft agar, and tumors

240 ion and cell proliferation between brands of tissue culture plates.

241 ifferent induction periods and using various tissue culture plates.

242 We describe herein a three-dimensional (3D) tissue culture platform using a polydimethylsiloxane (PD

243 Biomaterial-based tissue culture platforms have emerged as useful tools to

244 ed microbiological investigations (blood and tissue culture, plus tissue polymerase chain reaction [P

245 C-NSCs) cultured on PEG hydrogels or treated tissue culture polystyrene (TCP) surfaces.

246 ) depended on previous culture time on stiff tissue culture polystyrene (TCPS; E ~ 3 GPa).

247 em cells to densities comparable to those of tissue culture polystyrene controls (TCPS).

248 s within the tissue bulk severely limits the tissue culturing potential of many bioreactors.

249 iewpoint were used to compare periprosthetic tissues culture processes using conventional techniques

250 Plant tissue cultures (PTCs) provide researchers with unique m

251 could be purified and expanded in number in tissue culture, Richard Bunge in 1975 envisioned that th

252 ethod can be easily implemented in a typical tissue culture room by personnel with standard mammalian

253 NAs products in virus-free transgenic papaya tissue culture seedlings.

254 We made use of stable isotope labeling in tissue culture (SILAC) to identify ISGylated proteins th

255 healthy tissue from tumor tissue, and basic tissue culture skills.

256 duction time (which is in most cases free of tissue culture steps), and the implementation of confine

257 Both ex vivo stimulation and in vitro tissue culture studies demonstrated that the activation

258 Thus, although the majority of tissue culture studies have identified adders, our analy

259 l system for three-dimensional (3D) cell and tissue culture, studies of fibrillogenesis, and investig

260 lls were seeded on the AuNRs compared to the tissue-culture surface.

261 g the dissociated MSC aggregates onto planar tissue culture surfaces.

262 Our studies demonstrate that this primary tissue culture system enables one to study gastric tissu

263 The robotically handled whole-tissue culture system should help advance the developmen

264 Using a tissue culture system that supports the development of h

265 We used a primary tissue culture system to generate a murine p53 null gast

266 ng a mouse model of infection and a flexible tissue culture system.

267 on of models for SAC regulation developed in tissue culture systems and demonstrate that several fund

268 ectro-mechanical cardiac microenvironment in tissue culture systems favorable to high-resolution cell

269 burden to society, yet the lack of reliable tissue culture systems has hampered the development of a

270 chanistic inferences remains to be tested in tissue culture systems.

271 ood culture bottle system for periprosthetic tissue culture [T.

272 ssaged stocks of GPCMV are pathogenic, while tissue culture (TC) passage in fibroblasts results in at

273 Somatic embryogenesis is an important tissue culture technique that sometimes leads to phenoty

274 For conventional periprosthetic tissue culture techniques, the greatest accuracy was obs

275 we used a BTV8 strain minimally passaged in tissue culture (termed BTV8L in this study) and a deriva

276 hmallenberg virus (SBV) serially passaged in tissue culture (termed SBVp32) unexpectedly displayed in

277 h and without PD, as well as in interference tissue culture tests followed by multiple in-house and w

278 rmality is a somaclonal variant arising from tissue culture that drastically reduces yield, and has l

279 /Cas9-mediated genome editing requires plant tissue culture that is both time-consuming and genotype-

280 larized light backscattered from GNRs within tissue culture, the ensemble-averaged translational self

281 In tissue culture, TNC was superior amongst several extrace

282 We used three-dimensional tissue culture to build an organotypic model of bronchia

283 After passaging these viruses in tissue culture to select for functional variants, we use

284 level of adherence as those cells seeded on tissue culture-treated surfaces, by 4 d cell numbers and

285 ype compared with those cultured on standard tissue culture-treated surfaces.

286 en stimulation of T cells in ex vivo vaginal tissue cultures triggered antiviral responses in myeloid

287 in hamster cheek pouch topically exposed to tissue culture trypomastigotes (TCTs).

288 typic retinal explants after 1 to 3 weeks in tissue culture, under moderate glucose (MG) and HG condi

289 In tissue culture, VZV-infected human neurons remain viable

290 Tissue cultures was done on 86 patients; 3 (3%) were pos

291 ransgenic granule cells in mouse organotypic tissue cultures we found that spines containing SP survi

292 In aortic tissue culture, we found evidence that with aging mitoch

293 The tissue cultures were incubated at 37 degrees C in a 5% C

294 Results of tissue cultures were negative.

295 This effect was duplicated in tissue culture, where coculture of cancer cells with tum

296 Human corneal tissue culture with 100 microg/ml concentrations of SiNP

297 bine the facile manipulation and readouts of tissue culture with the virus-relevant complexity of ani

298 Compared with native tumors and tissue cultured with androgens, androgen deprivation in

299 Two or more positive periprosthetic tissue cultures with the same organism were considered a

300 As model systems, we use HeLa and HaCaT tissue cultures with water and with an aqueous DMSO solu