ライフサイエンスコーパス: total RNA

1 sativa L.) by high-throughput sequencing of total RNA.

2 erformed tunable hybrid capture of mRNA from total RNA.

3 lightly higher (by 1.6-fold) in mRNA than in total RNA.

4 tide specificity, and processes only 5 ng of total RNA.

5 mining only polyadenylated RNA and the other total RNA.

6 d well with profiles representing undepleted total RNA.

7 A libraries from a minute amount (500 pg) of total RNA.

8 tion of global microRNA (miRNA) abundance in total RNA.

9 leted of ribosomal RNA from only 1 microg of total RNA.

10 iological stains using as little as 50 pg of total RNA.

11 g capped RNA 5' ends from as little as 50 ng total RNA.

12 on average once every 35 uridine residues in total RNA.

13 collagen was examined by real-time RT-PCR on total RNA.

14 , H. pylori produced abundant ppGpp and less total RNA.

15 ing was performed using Affymetrix arrays on total RNA.

16 tect most isoacceptors from minute amount of total RNA.

17 itative method for single-cell sequencing of total RNA.

18 ty of sample types including human serum and total RNA.

19 nscriptome sequencing compared to 21% in the total RNA.

20 xon junction in complementary DNA from blood total RNA.

21 P-dependent increase in rRNAs but not in the total RNA 24 h after 5-HT treatment.

22 n S6(Ser235/236) (37%), greater rested-state total RNA (8.8%) and greater exercise-induced c-Myc mRNA

23 g RNA sequencing methods, we have determined total RNA abundance, transcription start sites, and tran

24 Associations between dose-dependent total RNA accumulation and increases in muscle mass and

25 This coincided with greater total RNA accumulation in the early phase of the trainin

26 This protocol uses as little as 10 ng of total RNA, allows multiplex sequencing of up to 96 sampl

27 We have developed a total RNA amplification and labeling strategy for use wi

28 )A sites in cells from as little as 10 ng of total RNA and can detect m(6)A accumulation in cells ove

29 leic acid purification, is used to pull-down total RNA and crosslinked RBPs, this method facilitates

30 Total RNA and double-stranded RNA (dsRNA) from mycelia a

31 nscript abundance even with small amounts of total RNA and effectively characterizes small samples ex

32 milar expression profiles were obtained with total RNA and enriched small RNA species (R(2) >or= 0.97

33 Total RNA and immunoprecipitated dsRNA from Escherichia

34 nanoliter volumes, using sequence from bulk total RNA and multiplexed quantitative PCR as benchmarks

35 We found that the total RNA and protein content, as well as the size of th

36 C activation has also been shown to increase total RNA and protein production in many tissue and dise

37 Total RNA and proteins were isolated from the membrane a

38 han afforded by the analysis of steady-state total RNA and should be useful in many biological settin

39 erase chain reaction signals per nanogram of total RNA and using NucleoSpin and mirVana columns is pr

40 synthetic mixtures of human genomic DNA and total RNA and with total RNA isolated from cells.

41 Protein, total RNA, and DNA were extracted from postmortem tempor

42 CR and Western blotting were performed using total RNA, and protein extracted from mouse CECs and hum

43 Genomic DNA, total RNA, and protein were isolated from mouse tissues

44 capped-small RNA-seq (csRNA-seq), which uses total RNA as starting material to detect transcription s

45 ed PA sorbent extracted sufficient mRNA from total RNA at concentrations as low as 5 ng muL(-1) in aq

46 were associated with greater accumulation of total RNA at Week 2 in the MOD leg, with every 1% differ

47 studies using a titration of mouse universal total RNA, BIIB outperformed commercially available kits

48 developed a method of isolating dsRNAs from total RNA by immunoprecipitation with a ds-RNA specific

49 eling and detection of small RNAs present in total RNA by splinted ligation.

50 nriched preparations were then obtained from total RNA by subtracting eucaryotic ribosomal and messen

51 at which activity was detected and increased total RNA cleavage at high Mg(2+) concentrations suffici

52 Total RNA collected from cultured islets was purified an

53 ibits a linear signal across a wide range of total RNA concentrations and agrees well with standard c

54 Although added total RNA concentrations increase tenfold, RNA concentra

55 acutely increases polyribosome occupancy of total RNA, consistent with an increase in mRNA translati

56 rial, methods were developed to: (1) isolate total RNA containing amplifiable mRNA from human skin an

57 ession (-64 +/- 5% vs. IGF-1; P < 0.001) and total RNA content (-16 +/- 2% vs. IGF-1; P < 0.001) in I

58 was associated with significant increases in total RNA content and protein metabolism.

59 ate with differences in proteasome activity, total RNA content, mRNA content, or cell division rate.

60 se transcription-PCR using G. sulfurreducens total RNA demonstrated that the genes encoding these thr

61 thium chloride or distilled water, extracted total RNA, depleted ribosomal RNA and performed whole-tr

62 d be reisolated in the cDNA pool or from the total RNA derived from the same or a different tissue, i

63 Total RNA derived from these cells of different confluen

64 ted with milk appears to be the isolation of total RNA directly from SC or MFG released into milk dur

65 ng the protocol for a commercially available total RNA/DNA extraction kit (RecoverALL).

66 a decreased capacity for protein synthesis (total RNA/DNA) and decreased sensitivity and capacity of

67 n and contributed the greatest proportion of total RNA-encoded protein expression, despite being the

68 ed using both methods from as little as 5 ng total RNA (~equivalent to 2 x 105 bacilli).

69 We assess and interpret changes in total RNA expression along developmental trajectories an

70 iked into hundreds of nanograms of the plant total RNA extract with a recovery below 110% using eithe

71 recently been assessed via pyrosequencing of total RNA extracted directly from natural microbial asse

72 eous detection of three endogenous miRNAs in total RNA extracted from 293T and HeLa cells.

73 med a whole-genome microarray analysis using total RNA extracted from actively growing broth cultures

74 Total RNA extracted from biopsies was subjected to next-

75 ng circulating miRNAs in serum and miRNAs in total RNA extracted from cancer cells.

76 y is conducted on the detection of miRNAs in total RNA extracted from cultured cells.

77 of circulating miRNAs in blood and miRNAs in total RNA extracted from cultured cells.

78 We applied RNA-Seq to total RNA extracted from HIV-2 blood plasma samples, dem

79 was applied for determination of miR-221 in total RNA extracted from human lung and breast cancer ce

80 Analysis of total RNA extracted from M. catarrhalis ATCC 43617 cells

81 separate short RNA libraries generated from total RNA extracted from M. truncatula leaves, represent

82 Total RNA extracted from plasma-derived EXOs of 12 T1DM

83 Total RNA extracted from the P7 lenses of Rho GDIalpha t

84 l-time reverse transcription-PCR analysis of total RNA extracted from the parental strain and csrA(Bb

85 eaction (RT-PCR) analysis of genomic DNA and total RNA extracted from the same sample before and afte

86 and cervical lymph nodes were harvested for total RNA extraction and gene expression by RT and real-

87 miRNA following DNA extraction as opposed to total RNA extraction for both blood- and saliva-specific

88 Endometrial biopsies were taken and total RNA extraction, cDNA synthesis and PCR was perform

89 supernatant of ocular fluid was subjected to total RNA extraction, followed by complementary deoxyrib

90 The cells were subjected to total RNA extraction, reverse transcription (RT), and re

91 fied the differential expression of miR21 in total RNA extracts from healthy breast tissue and diseas

92 trating expression profiling capabilities in total RNA extracts from the HL-60 cell line.

93 s can determine relative levels of miRNAs in total RNA extracts with sensitivity similar to small RNA

94 estimate the copy number range of miRNAs in total RNA extracts.

95 tely collected, can be used as the source of total RNA for QRT-PCR analysis.

96 hod includes a novel treatment that depletes total RNA fractions of highly abundant tRNAs and small s

97 reference sample consisting of a mixture of total RNA from 10 different normal human tissues not inc

98 To this end, we sequenced total RNA from 18 psoriasis patients and 16 healthy cont

99 Total RNA from 27 fresh tumor samples of 15 solid/multic

100 Initially, total RNA from 4 tissues samples per group was employed

101 The authors extracted total RNA from 60-day, 80-day, and 96-day human fetal re

102 Total RNA from 61 SCC samples and 10 matched normal lung

103 nt in total RNA from C. elegans and pRNAs in total RNA from bacteria.

104 method has become widely used for isolating total RNA from biological samples of different sources.

105 Total RNA from blood was processed on human Affymetrix m

106 de and can detect specific miRNAs present in total RNA from C. elegans and pRNAs in total RNA from ba

107 eveloped a method for preparing high-quality total RNA from Ca-alginate-encapsulated Saccharomyces ce

108 Total RNA from cardiac tissue of patients with dilated c

109 d miR-203 and miR-21 in samples of extracted total RNA from cell cultures.

110 Total RNA from cultured cementoblasts treated with 5 mM

111 sisRNA is detectable by RT-PCR in samples of total RNA from embryos up to the mid-blastula stage, whe

112 Moreover, total RNA from Escherichia coli, depleted of rRNA, also

113 suitable for isolating high yield and clean total RNA from field-grown plants.

114 Expression of miRNAs was determined using total RNA from formalin-fixed, paraffin-embedded tissue

115 Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels

116 similar m(6)A up-regulation was detected in total RNA from HIV-1-infected cells treated with a rever

117 In vitro, total RNA from hMSCs was extracted one week after cultur

118 RNase R digestion of the total RNA from human skeletal muscle generates an RNA po

119 his approach was applied to the detection of total RNA from human tissues and found to display differ

120 ntly increased m(6)A levels were detected in total RNA from Jurkat cells infected by single-cycle HIV

121 Total RNA from kidney biopsies was isolated at 3 clinica

122 Total RNA from lungs, hearts, kidneys, livers, and splee

123 se DNA microarrays were then used to analyze total RNA from M. catarrhalis cells grown in a continuou

124 RT-PCR of total RNA from M. nemestrina and Macaca fascicularis yie

125 Up to 75% of the total RNA from mullet ovaries was 5S rRNA.

126 Total RNA from ovaries of drought-stressed wild-type and

127 We compared gene expression on total RNA from patients' blood before and after treatmen

128 ene expression profiling was performed using total RNA from peripheral blood mononuclear cells (PBMCs

129 sine kinase receptor EphA4 were expressed in total RNA from Sezary cells and the paired amplified mRN

130 Expression profiling of total RNA from ten normal bone cell lines and eleven OGS

131 Northern blot analysis of total RNA from the O46E parent strain revealed a readily

132 Total RNA from the retina/(retinal pigment epithelium) w

133 With total RNA from the retina/(retinal pigment epithelium, R

134 le or no uspA2H transcript was detectable in total RNA from the UspA2H-negative variant O46E.U2V.

135 Total RNA from TM was used for quantitative PCR, while p

136 RT-PCR was performed on total RNA from wild-type mouse retina to identify the Dy

137 To define their compartmentalization, total RNA >100 nt was extracted from sonicated (SS) mous

138 e oligonucleotides have been synthesized and total RNA has been extracted, the procedure can be compl

139 ption (RT)-PCR and Northern blotting of lens total RNA, immunoblotting of lens membrane extracts, and

140 reverse transcriptase PCR (rRT-PCR), detect total RNA in a sample without differentiating vRNA from

141 lemented and evaluated; one was added to the total RNA in the samples before amplification and labeli

142 RNA-seq performed on total RNA indicated that only a fraction of total miRNAs

143 st one and up to four orders of magnitude of total RNA input and independent of sample composition.

144 t four targets, and requires just 10.3 ng of total RNA input in a 2 hour and 15 minutes assay.

145 sion profiles were generated with 100-200 ng total RNA input.

146 Two hundred picograms of total RNA is found to be sufficient for this analysis.

147 Our findings show that total RNA is sufficient to identify transcribed regulato

148 Total RNA is then recovered by precipitation with isopro

149 e method can be performed on crude lysate or total RNA, is fast, highly reproducible and minor change

150 Total RNA isolated from a tissue of interest is then seq

151 olymerase chain reaction were performed with total RNA isolated from blood cells of kidney graft reci

152 Using total RNA isolated from both E. coli and NTHi, we show f

153 of human genomic DNA and total RNA and with total RNA isolated from cells.

154 rse transcription of the mRNA portion of the total RNA isolated from each sample.

155 In an important validation study, total RNA isolated from four major chicken tissues: ceca

156 antly lower (by 6.5-43-fold) in mRNA than in total RNA isolated from HEK293T cells, whereas the level

157 miRNA microarray analyses of the hippocampal total RNA isolated from mice chronically treated with mu

158 ted nucleobase products (m(5)C and m(6)A) in total RNA isolated from mouse brain, pancreas, and splee

159 ic separation of picogram-nanogram levels of total RNA isolated from multiple cell types, including t

160 Total RNA isolated from PBMCs was hybridized onto the Af

161 Transcriptome sequencing of total RNA isolated from Tat- and vehicle-treated periphe

162 Total RNA isolated from whole blood of 26 un-medicated T

163 Transcriptional analyses of total RNAs isolated from intracellular DSM20231 and isog

164 Total RNAs isolated from renal proximal tubules (RPTs) o

165 was further improved by a modified method of total RNA isolation from serum/plasma, S/P miRsol, in wh

166 Data generated using the total RNA kit had more signal for introns and various RN

167 ols (ERCs), which can be either added to the total RNA level (tERCs) or introduced right before hybri

168 During meiosis, total RNA levels parallel 3' processing, which occurs in

169 5 NHP species and subspecies and constructed total RNA libraries for the same approximately 15 tissue

170 eveloped a novel single cell strand-specific total RNA library preparation method addressing all the

171 strategy for detecting 16S rRNA sequences in total RNA mixed samples extracted from the three pathoge

172 eply profile lncRNAs from polyadenylated and total RNA obtained from human neocortex at different sta

173 Gene expression was assessed on the total RNA of induced sputum (n = 83), nasal brushings (n

174 through 5-hydroxymethylcytidine (hm(5)C) in total RNA of mammalian cells.

175 We labeled total RNA of MEG-01 cells by incorporation of 5-ethynylu

176 time PCR from cDNA generated from intestinal total RNA or from RNA obtained by laser capture microdis

177 copies as the outcome variable and the input total RNA or plasma volume as an exposure variable, whic

178 in the presence of a million-fold excess of total RNA, paving the way for simple, point-of-care, low

179 which we extracted an average of 14.1 pg of total RNA per cell.

180 binding to DNA constrained by the values of total RNA polymerase (E) and sigma(70) per cell measured

181 nd a promoter complex with reduced levels of total RNA polymerase II (Pol II) and Pol II phosphorylat

182 xpressed in a cell type of interest from the total RNA pool from the tissue.

183 e RNA purification steps could influence the total RNA pool, we examined the impact of RNA isolation,

184 ype, transcript abundance in the nuclear and total RNA pools are highly correlated; whereas, in attpr

185 ated by RT-PCR and Northern blot analysis of total RNA preparations.

186 acids in abundant levels of genomic DNA and total RNA, processing of various sample types, and carry

187 transcripts accounted for up to one-third of total RNA reads from the infected-cell RNA population.

188 Under acidic conditions, total RNA remains in the upper aqueous phase, while most

189 and 44% present calls from 100 and 50 pg of total RNA, respectively.

190 Northern blots and RT-PCR analysis of total RNA revealed truncated transcripts that suggested

191 MiRNA-93 and miRNA-223 were determined by total RNA reverse transcription.

192 oxorubicin and performed RNA sequencing from total RNA (RNA-Seq) and AGO2-immunoprecipitated RNA (AGO

193 ogenous levels of the mature target miRNA in total RNA (RNAt) extracted from cancerous and non-cancer

194 the endogenous levels of both target miRs in total RNA (RNAt) extracted from metastatic breast cancer

195 monstrated by determining mature miRNA-21 in total RNA (RNAt) extracted from tumor cells and human ti

196 Attempts were made in profiling miRNAs in total RNA samples extracted from cancer cells and blood.

197 n a new biological dataset of 447 single-end total RNA samples from nasopharyngeal swabs, and establi

198 nt microarrays carrying tens of thousands of total RNA samples in the MAQC project on the study of re

199 llows the reproducible profiling of up to 24 total RNA samples within 24 h.

200 on of ten transcripts in two different human total RNA samples, we find good agreement between our si

201 ermination of target miRNA spiked into human total RNA samples.

202 ermination of target miRNA spiked into human total RNA samples.

203 Coupling total RNA-Seq and transcriptome re-assembly we annotated

204 Computationally identifying circRNAs from total RNA-seq data is a primary step in studying their e

205 The analyzed extensive set of total RNA-Seq data, together with the improvement of the

206 itive approach for predicting circRNAs using total RNA-seq data.

207 ipeline named UROBORUS to detect circRNAs in total RNA-seq data.

208 The SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Pico) by Ta

209 We demonstrate that our total RNA-seq method detects an equal or higher number o

210 zed prostate cancer, we performed ultra-deep total RNA-seq on 144 tumors with rich clinical annotatio

211 Total RNA-seq profiling of young (1 month old) and aged

212 etect circRNAs with low expression levels in total RNA-seq without RNase R treatment.

213 Inherent to total RNA-seq, our method is also able to detect circula

214 Using total RNA-Seq, we have found that transcription in 1-cel

215 ocus on transcriptome analysis, specifically total RNA sequencing (RNA-seq), by using monogenetic neu

216 Using total RNA sequencing and targeted diagnostic assays, we

217 in 130 introns of 115 Drosophila genes from total RNA sequencing data generated from developmental t

218 pecies of medically important mites based on total RNA sequencing data sets generated in this study a

219 We performed sRNA and total RNA sequencing from control and abiotically stress

220 Taken together, SMARTer single cell total RNA sequencing is very well suited for any single

221 Total RNA sequencing of liver biopsy specimens from the

222 Using total RNA sequencing, we found progressive disruption of

223 Total RNA-sequencing (RNA-seq) and comparative transcrip

224 mans with cocaine use disorder, we performed total RNA-sequencing on neuronal nuclei isolated from po

225 In total, RNA-sequencing data of 332 samples were used for

226 Northern blots from skeletal muscle total RNA showed severe reduction in abundance of mt-tRN

227 DNA hypermethylation and with a decrease in total RNA synthesis.

228 nd katG mRNA generated from 0.1 pg and 10 pg total RNA taken for NASBA, respectively, in less than 2

229 get preparation (by reverse transcription of total RNA), target-probe hybridization on array, signal

230 Additionally, the fraction of total RNA that phase-separates in vitro is sufficient to

231 racting 16 microg of mRNA from 315 microg of total RNA, the 0.4-microL volume monolith showed no sign

232 croRNA complement and sequenced libraries of total RNA to investigate the relationships with microRNA

233 ome analysis require a large amount of input total RNA to yield sufficient mRNA using either poly-A s

234 Here, we separately sequenced total RNAs (Total RNAseq) and mRNAs (mRNAseq) from the s

235 fied biosensing of mir21 from cell lysate of total RNA was demonstrated.

236 Total RNA was extracted and analyzed via quantitative (r

237 rols, and 22 rheumatic disease controls, and total RNA was extracted and reverse transcribed into com

238 blood was obtained before and after CRT, and total RNA was extracted for hybridization-based whole ge

239 Total RNA was extracted from 335 serum samples of HIV pa

240 Total RNA was extracted from exosomes purified from 152

241 Total RNA was extracted from formalin fixed paraffin emb

242 Total RNA was extracted from gingival biopsies from 26 p

243 Total RNA was extracted from gingival biopsy samples col

244 Total RNA was extracted from gingival crevicular fluid s

245 Total RNA was extracted from gingival tissue biopsies co

246 Total RNA was extracted from ileal biopsy specimens and

247 Total RNA was extracted from Sezary cells and amplified

248 Total RNA was extracted from the cells at baseline (cont

249 Next, total RNA was extracted from the tissue and analyzed wit

250 Their total RNA was extracted using a Trizol reagent.

251 ion and purified by centrifugation, and then total RNA was extracted using hot acid phenol.

252 From the peripheral blood cells, total RNA was extracted, quantified, and used for one-st

253 Efficient isolation of eukaryotic mRNA from total RNA was first mathematically modeled and then achi

254 Total RNA was harvested from the aortic rings, and repor

255 Labeled cRNA derived from TG-isolated total RNA was hybridized to 430 2.0 chips containing 14,

256 The resultant labeled cRNA from TG isolated total RNA was hybridized to gene microarray chips contai

257 Total RNA was isolated and assayed on whole genome micro

258 Total RNA was isolated and gene expression levels were d

259 At designated times, total RNA was isolated and gene expression was measured

260 Total RNA was isolated and reverse-transcribed.

261 Total RNA was isolated and used as a template in quantit

262 Total RNA was isolated at different time points followin

263 For expression studies, total RNA was isolated from 168 ileal biopsies of study

264 Total RNA was isolated from adipose tissue and adiponect

265 Total RNA was isolated from cell cultures and subjected

266 Total RNA was isolated from four biological replicates o

267 Total RNA was isolated from IL-1beta-induced and mock-in

268 Total RNA was isolated from KB cells 1 hour after treatm

269 Total RNA was isolated from microdissected dentate gyrus

270 Total RNA was isolated from PBMCs of five children with

271 Total RNA was isolated from the four treatment groups in

272 Total RNA was isolated from the human limbus and central

273 Total RNA was isolated from the MSCs on day 3.

274 Total RNA was isolated from TM cell strains, and mRNA ex

275 Total RNA was isolated from TM of cadaveric eyes derived

276 Total RNA was isolated from whole lung 24 and 48 h postf

277 Total RNA was isolated systematically throughout the int

278 Total RNA was isolated using TRIzol reagent from 42 appe

279 Total RNA was isolated, and qualitative RT-PCR was perfo

280 weeks, and 8 weeks after each treatment, and total RNA was isolated.

281 Total RNA was prepared using TRIzol reagent.

282 lens were dissected from each rat's eye, and total RNA was purified.

283 , LPS/IFN-gamma or IL4/IL13 respectively and total RNA was subjected to RNA-Sequencing (n = 3).

284 The isolated total RNA was then subjected to gene expression profilin

285 imary rat hippocampal neurons and sequencing total RNA, we found an unexpected set of free circular i

286 Total RNA were extracted and profiled on Affymetrix mous

287 Microbiota DNA and host total RNA were isolated from 189 bronchoalveolar lavages

288 Microbiota DNA and host total RNA were isolated from 203 bronchoalveolar lavages

289 The total RNAs were isolated and subjected to semiquantitati

290 Total RNAs were isolated for reverse transcription- quan

291 n and overall survival of patients with GBM, total RNAs were isolated from 268 FFPE tumor samples, mi

292 e background (5,000 to 10,000 copies per mug total RNA) were detected.

293 ength-controlled RNA fragments from purified total RNA, which can be easily detected by the biosensor

294 processed for histology or the isolation of total RNA, which was analyzed for differentially express

295 he detection limit was found to be 0.4 ng of total RNA, which was much lower than that obtained using

296 ary preparation kits (standard poly-A versus total RNA with Ribozero depletion) and analysis pipeline

297 pC-mt4 led to an increase in the recovery of total RNA with time in contrast to TA toxins that inhibi

298 tle as 10 pg of mRNA ( approximately 1 ng of total RNA) with this approach.

299 l RNAs from nanogram to microgram amounts of total RNA without an amplification step.

300 by directly analyzing a small amount of raw total RNA without enriching or fragmenting was also prel