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1 included the 16S rRNA gene and the internal transcribed spacer.
2 region spanning the promoter and 5' external transcribed spacer.
3 to the rDNA promoter and to the 5'- external transcribed spacer.
4 sts and, in phase 2, sequencing the internal transcribed spacers.
5 kinetoplast DNA (kDNA) PCR, nested internal transcribed spacer 1 (ITS-1) PCR, and a PCR-hybridizatio
6 fic probes were directed toward the internal transcribed spacer 1 (ITS-1) region and tested in a mult
7 the hypervariable sequences of the internal transcribed spacer 1 (ITS-1) region of the rRNA gene com
8 hological measurements of males and internal transcribed spacer 1 (ITS-1) sequences of rDNA between t
11 he mouse pre-rRNA transcript in the internal transcribed spacer 1 (ITS1) are affected by depletion of
13 5-ends at processing site A2 in the internal transcribed spacer 1 (ITS1) region of the rRNA primary t
14 fication (LAMP) assay targeting the Internal Transcribed Spacer 1 (ITS1) region of these pathogens fo
15 tion and sequencing of the D1D2 and internal transcribed spacer 1 (ITS1) regions of the nuclear ribos
16 M. intracellulare were confirmed by internal transcribed spacer 1 (ITS1) sequencing and characterized
17 generated by Rat1 digestion of the internal transcribed spacer 1 (ITS1) spacer from cleavage site A(
18 servation and molecular analysis of internal transcribed spacer 1 (ITS1), 18S ribosomal RNA (18S rRNA
20 equences from the 5'-ETS core, 18S, internal transcribed spacer 1 (ITS1), and 28S segments and also h
22 as confirmed by sequencing of the internally transcribed spacer 1 (ITS1)-5.8S-ITS2 rRNA-encoding regi
23 as confirmed by sequencing of the internally transcribed spacer 1 (ITS1)-5.8S-ITS2 rRNA-encoding regi
26 cation showed 100% concordance with internal transcribed spacer 1 (ITS1)/ITS2 sequencing and proved t
27 Using 16S ribosomal RNA (V1-V3) and internal transcribed spacer 1 amplicon sequencing analyzed with D
28 this cross-sectional study, we use internal transcribed spacer 1 amplicon sequencing to evaluate the
29 mic DNA from dust was extracted for internal transcribed spacer 1 Illumina MiSeq sequencing to identi
30 bind overlapping regions within the internal transcribed spacer 1, and both bind directly over cleava
31 RNA processing intermediate, the 5' internal transcribed spacer 1, indicate that bud23 mutants are de
33 subunit RNA gene (D1-D2 region) and internal transcribed spacers 1 and 2 (ITS1 and ITS2 regions), hav
34 nd Tibet, and the nuclear ribosomal internal transcribed spacer-1 sequences from each sample were obt
36 rs to amplify a segment of the rRNA internal transcribed spacer 2 (ITS2) from multiple Leishmania spe
37 ribosomal DNA (rDNA) and 5.8S rDNA/internal transcribed spacer 2 (ITS2) Malassezia-specific PCR prim
38 9 polynucleotide kinase to form the internal transcribed spacer 2 (ITS2) pre-rRNA endonuclease-kinase
39 nventional PCR amplification of the internal transcribed spacer 2 (ITS2) region (ITS2-PCR) followed b
42 on of sequence polymorphisms in the internal transcribed spacer 2 (ITS2) region of the rRNA genes as
43 ific polymorphisms in the noncoding internal transcribed spacer 2 (ITS2) region of the rRNA operon pr
44 found that sequence analysis of the internal transcribed spacer 2 (ITS2) region provided further iden
46 exit, and the domain including the internal transcribed spacer 2 (ITS2) that separates 5.8S and 25S
47 tochrome c oxidase subunit 1 (COI), internal transcribed spacer 2 (ITS2), and tyrosine hydroxylase (T
48 y of these proteins bind at or near internal transcribed spacer 2 (ITS2), but in their absence, ITS1
50 e genotyping analysis targeting the Internal transcribed spacer 2 of rDNA, we report for the first ti
51 encing analysis of a portion of the internal transcribed spacer 2 region (ITS2) for identification of
53 onucleotide probes, directed to the internal transcribed spacer 2 region of ribosomal DNA from Asperg
55 Next-generation metagenomics and internal transcribed spacer 2 sequencing delineated fecal bacteri
56 for the endonucleolytic cleavage in internal transcribed spacer 2 that separates the 5.8S rRNA from t
57 ear genetic loci such as 5.8S rRNA, Internal Transcribed Spacer 2, and 28S rRNA regions, which are co
58 g amplicon sequencing of the fungal internal transcribed spacer 2, we studied the root and rhizospher
59 Independently, the ribosomal DNA internal transcribed spacer-2 (ITS-2) regions from these species
64 olic controls using fungal-specific internal transcribed spacer amplicon sequencing of fecal samples.
67 ed by DNA sequencing using both the internal transcribed spacer and D1/D2 region of the 28S ribosomal
68 uences and the boundary between the external transcribed spacer and the 18S coding sequence in a clon
69 owed that a large segment of the 5' external transcribed spacer and the entire first internal transcr
70 biogenesis complexes assist the 5' external transcribed spacer and U3 small nucleolar RNA in providi
71 terminator sequences are present in rDNA non-transcribed spacers and a region immediately preceding t
72 , and also sequence analysis of the internal-transcribed-spacer and D1/D2 rDNA regions, the yeast was
73 C-01 nucleotide sequences (gltA and internal transcribed spacer) and protein band banding pattern wer
74 nteractions, designated the U3-ETS (external transcribed spacer) and U3-18S duplexes, are essential t
75 ication initiates from origins in the 5' non-transcribed spacer, and forks moving toward the center o
76 , the major SIR-Responsive Region in the non-transcribed spacer, and SRR2, in the 18S rRNA coding reg
78 e identified by sequencing the 5.8S internal transcribed spacer as Pichia fermentans, Wickerhamomyces
80 scribed spacer and the entire first internal transcribed spacer, both of which flank 18S rRNA, are no
84 h mitochondrial (cox1) and nuclear (internal transcribed spacer) DNA data from the Schistosoma eggs o
85 is in turn implies that large regions of the transcribed spacers do not play a sequence-specific role
86 osomal and transfer RNA maturation, external transcribed spacer (ETS) and internal transcribed spacer
87 nd the subsequent removal of the 3' external transcribed spacer (ETS) at the dense fibrillar componen
89 did not contain the short-lived 5'-external transcribed spacer (ETS) leader segment upstream from th
90 of by-products derived from the 5'-external transcribed spacer (ETS) of 45S pre-rRNA, as MTR4 does.
92 u hybridization with a probe to the external transcribed spacer (ETS) region of the pre-rRNA shows th
94 ge and complementary regions in the external transcribed spacer (ETS); these interactions are phyloge
95 better target than the 16S-23S rRNA internal transcribed spacer for DNA sequence-based species identi
97 necessary for the removal of the 3' external transcribed spacer from 28S rRNA and productive downstre
98 is required for cleavage of the 3' external transcribed spacer from unprocessed pre-rRNA and for pro
99 biota composition was determined by internal transcribed spacer gene sequencing in HDs (n = 23) and p
100 d of PAM based on sequencing of the internal transcribed spacers, including the 5.8S rRNA genes.
103 olds were sequence identified using internal transcribed spacer (ITS) and 28S (yeasts) or ITS, transl
104 made using two genetic markers, the internal transcribed spacer (ITS) and a fragment of the beta-tubu
105 ofile, and Sanger sequencing of the internal transcribed spacer (ITS) and D1/D2 regions of the riboso
106 creened by sequence analysis of the internal transcribed spacer (ITS) and D1/D2 ribosomal DNA regions
107 ing tool with great accuracy, while internal transcribed spacer (ITS) and D1/D2 sequencing were succe
108 ic range of the tribe, with nuclear internal transcribed spacer (ITS) and external transcribed spacer
109 ndent set of genotypes for both the internal transcribed spacer (ITS) and large subunit (LSU) ribosom
110 t amplicon sequencing of the fungal internal transcribed spacer (ITS) and prokaryotic 16S DNA regions
111 is, and Debaryomyces fabryi using intergenic transcribed spacer (ITS) and/or intergenic spacer (IGS)
112 encing methods with confirmation by internal transcribed spacer (ITS) and/or partial 16S rRNA gene se
117 Fungi were isolated, and a fungal internal transcribed spacer (ITS) clone library survey was perfor
118 bacterial 16S rRNA gene and fungal internal transcribed spacer (ITS) copy numbers and extracellular
119 ermatrix data set was combined with internal transcribed spacer (ITS) data sets for Astraeus, Calosto
120 f ECM fungi was determined using an internal transcribed spacer (ITS) database terminal restriction f
121 sequence analysis of the ribosomal internal transcribed spacer (ITS) from specimens obtained from hu
123 g to isolate and characterize nrDNA internal transcribed spacer (ITS) homeologues from multiple acces
126 DNA barcoding of nuclear ribosomal internal transcribed spacer (ITS) of the rRNA gene with fungal sp
127 n the number of GTTT repeats in the internal transcribed spacer (ITS) of the rRNA have been described
129 subunit (SSU) rRNA genes and fungal Internal Transcribed Spacer (ITS) rDNA sequences, samples contain
130 conserved portion of the T. foetus internal transcribed spacer (ITS) region (ITS1 and ITS2) and the
131 0% in agreement with the contiguous internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) sequenc
133 g 16S ribosomal RNA (rRNA) gene and internal transcribed spacer (ITS) region amplicon sequencing, we
135 h included sequence analysis of the internal transcribed spacer (ITS) region and a fragment of the la
136 It targets the nuclear ribosomal internal transcribed spacer (ITS) region and offers nearly 10 mil
138 f PCR products generated from the intergenic transcribed spacer (ITS) region did not differentiate am
139 R targeting the 16S-23S rRNA gene intergenic transcribed spacer (ITS) region has been proposed as a r
140 gions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probabil
143 mparing their DNA sequences for the internal transcribed spacer (ITS) region of the 18S-26S ribosomal
145 , a nested PCR method targeting the internal transcribed spacer (ITS) region of the rRNA operon was v
147 PCR assay targeting the 16S-to-23S internal transcribed spacer (ITS) region with use of MGB Eclipse
148 The genetic markers used were the internal transcribed spacer (ITS) region, and fragments of the be
151 ungal barcode-the nuclear ribosomal internal transcribed spacer (ITS) region-and offers all ~1 000 00
154 n of the 28S ribosomal gene and the internal transcribed spacer (ITS) regions 1 and 2 of the rRNA ope
155 nce analysis of the ribosomal DNA intergenic transcribed spacer (ITS) regions and the D1-D2 variable
158 ty of sequences of the nuclear rDNA internal transcribed spacer (ITS) regions for phylogenetic analys
159 The secondary structure of the internal transcribed spacer (ITS) regions of nuclear rRNA transcr
160 uence analysis of the hypervariable internal transcribed spacer (ITS) regions of ribosomal DNA (rDNA)
162 ysis of the large subunit (LSU) and internal transcribed spacer (ITS) regions of the nuclear ribosoma
163 sequence analysis that included the internal transcribed spacer (ITS) regions of the nuclear ribosoma
164 n was achieved by sequencing of the internal transcribed spacer (ITS) regions of the rRNA gene and by
165 tube nested PCR which amplifies the internal transcribed spacer (ITS) regions of the rRNA genes of hu
166 nce variations in the ITS1 and ITS2 internal transcribed spacer (ITS) regions of the rRNA genes were
167 n of the secondary structure of the internal transcribed spacer (ITS) regions separating nuclear ribo
168 ific primers were designed from the internal transcribed spacer (ITS) regions, ITS1 and ITS2, of the
169 as identified by fungal culture and internal transcribed spacer (ITS) ribosomal DNA (rDNA) sequencing
170 ence analysis of the 283-bp 16S-23S internal transcribed spacer (ITS) sequence showed only 95% identi
171 heir origin was traceable via their internal transcribed spacer (ITS) sequence to five distinct Panic
172 id and nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS) sequence variation within the C
173 ternal transcribed spacer (ETS) and internal transcribed spacer (ITS) sequences are excised and, as n
174 ylogenetic analysis of nuclear rDNA internal transcribed spacer (ITS) sequences from a worldwide samp
177 t sequencing for the 16S-23S rRNA internally transcribed spacer (ITS) to examine ecotype and fine-sca
178 The identification of P. jirovecii internal transcribed spacer (ITS) types was performed on P. jirov
179 sed variation in nuclear sequences (internal transcribed spacer (ITS)) and two types of chloroplast D
180 arkers used for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large
181 ress this question using the fungal internal transcribed spacer (ITS), which is central in many phylo
186 ucted using sequences from nuclear (internal transcribed spacer, ITS; and alcohol dehydrogenase 1A, A
187 it of ribosomal RNA (rRNA), and the internal transcribed spacer ITS1 of rRNA established an order for
188 r distinct sites located within the internal transcribed spacers ITS1 and ITS2 and the 3' external sp
189 tial sequences of nuclear ribosomal internal transcribed spacers (ITS1 and ITS2) and intergenic space
191 zed as a single transcript with two internal transcribed spacers (ITS1 and ITS2), which are removed b
192 e preferentially cleaved the second internal transcribed spacer (ITS2) approximately 250 nt downstrea
194 lear small-subunit rDNA, and second internal transcribed spacer, mitochondrial large-subunit rDNA, an
196 ribed spacers (ITS-1 and 5' ETS) and the non-transcribed spacer (NTS) or intergenic spacer (IGS) form
197 Sequence analysis of the ribosomal internal transcribed spacer of 56 Mycobacterium avium complex iso
198 U3 subsequently base pairing to the external transcribed spacer of pre-rRNA, thus positioning U3 snoR
199 S ribosomal RNA, D2-D3 of 28S rRNA, internal transcribed spacer of rRNA, mitochondrial cytochrome oxi
202 confirmed to be Sporothrix genus by internal transcribed spacer or beta-tubulin PCR sequencing were i
203 ts containing sequences from the 5' external transcribed spacer or the first internal transcribed spa
204 th polymorphisms of PCR products (intergenic transcribed spacer PCR [ITS-PCR] ribotyping) could disti
205 evidence for recombination in their internal transcribed spacer profiles indicates that they are of r
208 s performed by amplification of the internal transcribed spacer region (ITS) that contained the targe
209 ation and genomic sequencing of the internal transcribed spacer region (ITS), partial beta-tubulin (B
210 cleotide sequence variations in the internal transcribed spacer region 1 (ITS1) and region 2 (ITS2) o
214 g (~80-90%) and members of the same internal transcribed spacer region 2 (ITS2) type were phylogeneti
215 base-pair fragments of DNA from the internal transcribed spacer region 2 from 28 historic herbarium s
217 ncing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly disting
219 6S amplicon sequencing) and fungal (internal transcribed spacer region amplicon sequencing) communiti
221 s of the following three genes: the internal transcribed spacer region and domains D1 plus D2 of the
223 targets including the 16S-23S rDNA internal transcribed spacer region and the rpoB gene (partial seq
224 clade, large-subunit ribosomal and internal transcribed spacer region DNA sequences were determined
225 utility of sequence analysis of the internal transcribed spacer region is highlighted; however, furth
226 ribosomal DNA probe specific to the external transcribed spacer region located at the 5' end of the r
227 at the two tandem termini present in the non-transcribed spacer region located between the sequences
228 alysis based on the sequence of the internal transcribed spacer region of fungal ribosomal RNA encodi
229 phylogeny based on sequences of the internal-transcribed spacer region of nuclear ribosomal DNA to tr
230 irected at the amplification of the internal transcribed spacer region of the Mycobacterium genome wi
232 agment length polymorphism of the internally transcribed spacer region of the rRNA operon (ITS PCR-RF
233 ribosomal DNA probe specific to the external transcribed spacer region or to the 28S region of the ri
234 um using 16S ribosomal RNA gene and internal transcribed spacer region sequencing to profile bacteria
235 fied polymorphic DNA analysis and internally transcribed spacer region sequencing, by testing species
236 he 16S rRNA gene, 18S rRNA gene and internal transcribed spacer region sequencing, we analysed multip
238 con sequencing of the ribosomal RNA internal transcribed spacer region to examine the microdiversity
239 P. gingivalis-specific amplification was the transcribed spacer region within the ribosomal operon.
240 f the rRNAs (5S, 16S and 23S), an internally transcribed spacer region, and the number of tRNA genes.
241 the bacterial 16S rRNA and fungal internally transcribed spacer region, as well as bacterial genus-sp
245 specific sequence, belonging to the Internal Transcribed Spacers region, was used to design the real-
246 isms (RFLPs) of the PCR-amplified intergenic transcribed spacer regions (including the 5.8S ribosomal
248 of the nuclear ribosomal RNA (rRNA) internal transcribed spacer regions (ITS1 and -2) to detect and d
249 nomic assertions based on ribosomal internal transcribed spacer regions (ITS1/2) and expanded protein
250 sm (TRFLP) and sequencing of cloned internal transcribed spacer regions and 16S rRNA genes, respectiv
251 Here we use analyses of the nuclear internal transcribed spacer regions and other genetic traits to r
252 eaction primer set that targets the internal transcribed spacer regions between conserved bacterial g
253 porulating molds (NSM).We sequenced internal transcribed spacer regions from 50 cultures of NSM and f
254 cleotide sequence variations in the internal transcribed spacer regions I and II (ITS1 and ITS2, resp
256 sing sequences of both ndhF and the internal transcribed spacer regions of nuclear ribosomal DNA reve
258 Target sequences in the noncoding internal transcribed spacer regions of the rRNA operon were simul
259 tive nucleotide bases in repetitive internal transcribed spacer regions of the rRNA-encoding DNA (rDN
260 rmatitidis; for 5, we sequenced the internal transcribed spacer regions, and for 4 others the whole g
261 rmatitidis; for 5, we sequenced the internal transcribed spacer regions, and for the other 4 we seque
262 the D1/D2 region of ribosomal DNA, internal transcribed spacer regions, and intergenic spacer region
263 e of 18S and 28S ribosomal genes, internally transcribed spacer regions, and mitochondrial genes.
266 lar pre-ribosomal assembly - the 5' external transcribed spacer ribonucleoprotein - provides a mechan
267 ome requires the presence of the 5' external transcribed spacer RNA and all ribosomal RNA domains.
268 ents adjacent to the telomere, followed by a transcribed Spacer sequence, a G-rich microsatellite and
270 od phylogenetic methods to identify internal transcribed spacer sequences from the UNITE database for
276 riome (16S rRNA) and mycobiome (18S Internal Transcribed Spacer) sequencing (total, 228 microbiomes).
277 production is sustained upon introduction of transcribed spacers that reposition SNV RU5 35 to 200 nu
278 nal transcribed spacer or the first internal transcribed spacer, the enzyme preferentially cleaved th
279 the nucleotide sequences of the two internal transcribed spacers, the adjacent ribosomal coding seque
280 fication, zinc finger protein, rRNA external transcribed spacer, thymosin beta-4, cyclin B1 and sever
281 le regions (e.g. 16S rRNA or fungal Internal Transcribed Spacer) to assess diversity or compare popul
282 e of this study was to evaluate the Internal Transcribed Spacer units 1 and 2 (ITS) of the rDNA opero
283 regions of chloroplast DNA and rDNA internal transcribed spacer were incongruent in most New World sp
284 tion of pre-ribosomal RNA at the 5' external transcribed spacer, which directs the early association
286 tes by DNA sequence analysis of the internal transcribed spacer with or without D1/D2 ribosomal RNA r