ライフサイエンスコーパス: transcription system

1 gene activation in a physiologically intact transcription system.

2 ional repression in a reconstituted in vitro transcription system.

3 tion directly in a highly purified cell-free transcription system.

4 orientation-dependent manner in a cell-free transcription system.

5 ter using a human in vitro RNA polymerase II transcription system.

6 scription in a HeLa nuclear extract in vitro transcription system.

7 n in a reconstituted human RNA polymerase II transcription system.

8 ation of the ctaA promoter using an in vitro transcription system.

9 activity), we utilized the heterologous Gal4 transcription system.

10 ere analyzed using an in vitro reconstituted transcription system.

11 3 inhibits rRNA transcription in a cell-free transcription system.

12 us major late promoter in a minimal in vitro transcription system.

13 ng transcript produced in a defined in vitro transcription system.

14 fter transcription using a T7 RNA polymerase transcription system.

15 huronic acid synthesis, by using an in vitro transcription system.

16 ed in a well defined human RNA polymerase II transcription system.

17 s able to mediate activator function in this transcription system.

18 ription process in a highly defined in vitro transcription system.

19 , was analyzed using a synchronized in vitro transcription system.

20 oter in a highly purified HeLa reconstituted transcription system.

21 velopment of a gp67-responsive E. coli-based transcription system.

22 n, in a yeast-derived TBP-dependent in vitro transcription system.

23 n vitro in a reconstituted RNA polymerase II transcription system.

24 siae Leu3 protein (Leu3p) in a reconstituted transcription system.

25 nterference between PR and TR in a cell-free transcription system.

26 repeat using an in vitro reconstituted yeast transcription system.

27 investigate the role of BRCA1 in a cell-free transcription system.

28 mpediments to the performance of the T7 RNAP transcription system.

29 racts as well as in a reconstituted in vitro transcription system.

30 chaeal RNAP in a wholly recombinant in vitro transcription system.

31 in a biochemically defined RNA polymerase II transcription system.

32 n promoters, we used a highly purified human transcription system.

33 emplate using a highly purified human pol II transcription system.

34 transcribe through nucleosomes in a defined transcription system.

35 ivate transcription in a reconstituted human transcription system.

36 Rgamma function in a reconstituted cell-free transcription system.

37 tors TFIIF and TFIIS in the analogous Pol II transcription system.

38 ntral feature into an otherwise heterologous transcription system.

39 cially activators, regulate a eukaryote-like transcription system.

40 imentary basal initiation apparatus for each transcription system.

41 lementable within the much simpler bacterial transcription system.

42 ells and produce nascent RNAs in an in vitro transcription system.

43 has come recently from studies in mammalian transcription systems.

44 pendent transcription in mammalian cell-free transcription systems.

45 hted a conserved core shared among all three transcription systems.

46 ases I, II and III than it is to eubacterial transcription systems.

47 Fis in both site-specific recombination and transcription systems.

48 h can be extended to study other viruses and transcription systems.

49 ts between proteins from the translation and transcription systems.

50 Using the in vitro transcription system, a minimal core promoter of 60 bp (

51 In all three transcription systems, a key step in the activation proc

52 ndent transcription in a homologous in vitro transcription system, accumulates in nuclei of phloem an

53 Here, using an in vitro transcription system and a series of deletion mutants of

54 e elongation we optimized a defined in vitro transcription system and compared results obtained with

55 ctivity occurs in both a typical prokaryotic transcription system and in a eukaryotic-like bacterial

56 transcriptional activity both in an in vitro transcription system and in living cells, which is in co

57 elf were assayed in vitro by using a minimal transcription system and in vivo by assaying beta-galact

58 ed transcription in a well-defined cell-free transcription system and interacted specifically with th

59 assayed as a Gal fusion in the reconstituted transcription system and interacts both with TATA-bindin

60 promoter in a highly purified, reconstituted transcription system and that RA-inducible expression of

61 nduction of the antioxidant response element transcription system and the inhibition of the transcrip

62 activation by liganded TR in the frog oocyte transcription system and was recruited to the T3 respons

63 Data from reconstituted cell-free transcription systems and binary interaction assays sugg

64 to a broad variety of bacteriophage in vitro transcription systems and provides a platform for develo

65 ons of the chemical master equation for such transcription systems as a function of time, we here dev

66 te 5S gene transcription in a rodent RNAPIII transcription system, as did Xenopus TFIIIA.

67 ucted a red light-sensitive Escherichia coli transcription system based on a chimera between the red/

68 y DNAzymes or ribozymes, this ATP- initiated transcription system based on the T7 phi2.5 promoter can

69 studied nuclear, bacterial, or bacteriophage transcription systems but that similarities are found on

70 -less transcription in a Drosophila in vitro transcription system, but the mechanism responsible for

71 eveloped a thyroid hormone dependent in vivo transcription system by introducing TRs and RXRs (9-cis-

72 ransfected hepatoma cells and in a cell-free transcription system by the binding of factors to this D

73 t biochemically defined reconstituted Pol II transcription systems can be used to investigate how, wh

74 by human c-Jun in a human RNA polymerase II transcription system composed of highly purified recombi

75 chromatin templates assembled in vitro and a transcription system composed of the human general trans

76 Using a minimal in vitro transcription system consisting of a tna template, RNA p

77 l activation, we established a reconstituted transcription system consisting of human components that

78 imately P in intact cells and in an in vitro transcription system consisting of purified bacterial co

79 ntial terminators was observed in a purified transcription system consisting of template, polymerase,

80 clobutane pyrimidine dimer using an in vitro transcription system consisting of templates containing

81 ds and the phytonutrient regulators of these transcription systems contain electrophilic active group

82 stituted a highly purified RNA polymerase II transcription system containing chromatin templates asse

83 ptor-mediated silencing, we used a cell-free transcription system containing HeLa nuclear extracts in

84 I) was examined in a highly defined in vitro transcription system containing Pol II and purified fact

85 sh DPE-specific transcription in an in vitro transcription system containing TFIID, Mediator, and the

86 transcription, we have developed an in vitro transcription system derived from bovine retinal nuclear

87 Using a highly pure transcription system derived from Saccharomyces cerevisi

88 An in vitro transcription system derived from the ssu72-2 mutant exh

89 these mutants was examined both in cell-free transcription systems derived from hepatoma (HepG2) and

90 purified from wild-type cells in an in vitro transcription system designed to assay active Pol I-Rrn3

91 domains in a highly purified human cell-free transcription system devoid of TFIIA and Mediator.

92 ified LRPPRC to a recombinant human in vitro transcription system did not activate mtDNA transcriptio

93 In the frog oocyte transcription system, dpTR bound a T3-responsive promote

94 and thereby examines the homeostasis of the transcription system during EMT.

95 omparable to that obtained with the in vitro transcription system employed here.

96 transition during the evolution of eukaryal transcription systems, exhibiting a relatively complete

97 ed transactivation, we developed a cell-free transcription system for 1,25(OH)2D3 signaling by utiliz

98 An in vitro transcription system for RNA polymerase I-catalyzed RNA

99 Until now, however, a defined in vitro transcription system for the biochemical study of develo

100 here the development of a cell-free in vitro transcription system for the detection of specific targe

101 e, modular, versatile CRISPR-based synthetic transcription system for the programmable control of gen

102 ssay, making this the first general in vitro transcription system for the simultaneous analysis of al

103 g a faithful recombinant human mitochondrial transcription system from Escherichia coli, we demonstra

104 Here we developed an in vitro transcription system from the yeast Saccharomyces cerevi

105 The development of a recombinant in vitro transcription system has allowed for a detailed molecula

106 The development of a reconstituted chromatin transcription system has allowed us to isolate a novel c

107 ity of environmental cues that the bacterial transcription system has evolved to respond.

108 pret these results to mean that the archaeal transcription system has retained more ancestral charact

109 ort hairpin RNAs via the use of PolIII-based transcription systems has proven to be an effective mech

110 ts produced from them in homologous in vitro transcription systems have been determined.

111 d in the human immunodeficiency virus type 1 transcription system, hnRNP U inhibits elongation rather

112 t the development of a green light-inducible transcription system in E. coli based on a recently disc

113 capitulated in this highly purified in vitro transcription system in the absence of TAFIIs.

114 ence points to a larger role for the Pol III transcription system in various other nuclear processes,

115 anscription with the highly resolved Pol III transcription system in vitro was also diminished when r

116 We used a reconstituted transcription system in vitro with purified polymerase a

117 tant SRC-1 with liganded PR in the chromatin transcription system in vitro, the basic helix-loop-heli

118 and mutant versions of p300 with a chromatin transcription system in vitro.

119 We established an in vitro transcription system in which both 3' end processing and

120 conclusion comes from use of an in vitro GAS transcription system in which CovR was sufficient to med

121 is a special cytoplasmic membrane-associated transcription system in which DNA-dependent RNA polymera

122 RNA polymerase II, we developed a chromatin transcription system in which periodic nucleosome arrays

123 These compounds markedly potentiate chimeric transcription systems in cell-based assays and strikingl

124 esults indicate the presence of TATA-unified transcription systems in contemporary eukaryotes and pro

125 fluorescence-based readouts of more complex transcription systems in vitro.

126 ntitermination occurs in vitro in a purified transcription system, in the absence of ribosomes or acc

127 A eukaryotic transcription system including AID has not been reported

128 e within the cytoplasm and encode a complete transcription system, including a multisubunit RNA polym

129 Using a highly reconstituted chromatin-transcription system incorporating the inducible RARbeta

130 btained in a reconstituted RNA polymerase II transcription system, indicated that the promoter escape

131 se) can be reproduced in a purified in vitro transcription system, indicating that the nascent transc

132 A cell-free transcription system involving immobilized DNA templates

133 Drosophila TFlIB, a highly purified in vitro transcription system is generated that has not previousl

134 Addition of crude cell extract to the simple transcription system leads to repression of all three pr

135 r or linear DNA was incubated in an in vitro transcription system made from a whole-cell extract of H

136 s, we speculate that the human mitochondrial transcription system may have evolved to differentially

137 of SRC-1 and SMRT contained in our chromatin transcription system modulates agonist/antagonist effect

138 Like the more complex multisubunit transcription systems, multiple steps may exist for cont

139 l nuclear extract (NE) based in vitro runoff transcription system of core beta-pol promoter human DNA

140 This study provides new insights into the transcription system of euglenoid plastids, the organiza

141 Previously, the in vitro late transcription system of vaccinia virus was resolved into

142 tion in a unidirectional manner in a HeLa mt transcription system, only in the presence of purified m

143 ribe the development of a versatile in vitro transcription system optimized for the expression of MSS

144 or in the reconstituted frog oocyte in vivo transcription system, overexpression of Dot1L enhances g

145 nscribed in the well-characterized cell-free transcription system prepared from HeLa nuclei.

146 ffects on basal transcription in an in vitro transcription system reconstituted from purified compone

147 ion was studied in a human RNA polymerase II transcription system reconstituted from recombinant and

148 his study, we describe a mammalian cell-free transcription system reconstituted with only recombinant

149 ter escape by RNA polymerase II in a minimal transcription system reconstituted with purified polymer

150 in the absence of an ATP cofactor in a basal transcription system reconstituted with purified RNA pol

151 llenge assays performed in a human cell-free transcription system reconstituted with recombinant gene

152 pends strongly on ATP, TFIIE, and TFIIH in a transcription system reconstituted with RNA polymerase I

153 However, in vitro transcription systems reconstituted from homogeneous pre

154 We first compared Eve activities in in vitro transcription systems reconstituted with either all the

155 phoretic mobility shift assays and cell-free transcription systems reconstituted with purified genera

156 tivation of the bvgR promoter in an in vitro transcription system requires the addition of phosphoryl

157 For example, in the yeast rDNA transcription system, Rrn3 might function catalytically,

158 an essential component of all three nuclear transcription systems, sharply kinks the TATA box at two

159 Further dissection of this in vitro transcription system should be highly useful toward eluc

160 Use of this in vitro transcription system should permit analysis of the funct

161 of the promoter in an in vitro reconstituted transcription system shows that CBF activates transcript

162 quired for NFATp-mediated activation in this transcription system, since TATA-binding protein (TBP) a

163 f recombinant mutants in a reconstituted RSV transcription system suggested that the divalent-cation-

164 Bacterial enhancer-dependent transcription systems support major adaptive responses a

165 e development of a transcription and reverse-transcription system that can replicate unnatural geneti

166 been investigated using a purified in vitro transcription system that does not assemble chromatin.

167 hese findings reveal a specialized TCT-based transcription system that is directed toward the synthes

168 ed with recombinant Drosophila TBP to give a transcription system that is nearly free of contaminatin

169 anscription, we have established an in vitro transcription system that is responsive to large T antig

170 Using a biochemically defined in vitro transcription system that mediates OCT4/SOX2 and coactiv

171 re, we established a MyoD-dependent in vitro transcription system that permits us to determine the ro

172 Employing an in vitro chromatin transcription system that recapitulates progesterone rec

173 Employing a cell-free chromatin transcription system that recapitulates progesterone rec

174 is formed as a result of the self-organizing transcription system that regulates GLT expression and m

175 port the development of a cell-free in vitro transcription system that uses RNA Output Sensors Activa

176 n alternations in expression in the in vitro transcription system that were predicted by the mechanis

177 NF-4 functions, we have established in vitro transcription systems that faithfully recapitulate HNF-4

178 he cells undergo a switch from a TFIID-based transcription system to a TRF3-TAF3-based system.

179 We used a rice whole-cell extract in vitro transcription system to characterize the functional inte

180 have used an in vitro chromatin assembly and transcription system to compare the transcriptional acti

181 e have previously used a homologous in vitro transcription system to define functional elements of th

182 ing a highly purified reconstituted in vitro transcription system to demonstrate how pRB can repress

183 ties as DNA templates in a purified in vitro transcription system to demonstrate transcriptional coup

184 argely in exon 1, we established an in vitro transcription system to evaluate activation of transcrip

185 have used an in vitro chromatin assembly and transcription system to examine the biochemistry of inte

186 fide prokaryotes but employ a eukaryote-like transcription system to express the information of bacte

187 Additionally, we have developed an in vitro transcription system to generate RNase-resistant RNA tem

188 We developed a single-molecule fluorescence transcription system to investigate TFIIB release in vit

189 advantage of an in vitro reconstituted yeast transcription system to investigate the underlying mecha

190 We devised an in vitro transcription system to study specific Pol II terminatio

191 We have used an in vitro transcription system to study the roles of CatR, cis,cis

192 including an in vitro chromatin assembly and transcription system, to examine the effects of histone

193 including an in vitro chromatin assembly and transcription system, to examine the functional role for

194 Using a reconstituted transcription system together with recombinant or endoge

195 transfected cells (TRalpha) and in cell free transcription systems (TRalpha and vitamin D receptor).

196 l promoter system in which a tissue-specific transcription system under the control of a cancer-speci

197 We have developed an in vitro transcription system, using HeLa nuclear extract, that s

198 , the template that was used in the in vitro transcription system was a large covalently, closed circ

199 An in vitro transcription system was applied, which utilizes T7 RNA

200 A glucocorticoid-inducible transcription system was employed to control the express

201 A DNA replication-independent late gene transcription system was established by cotransfecting p

202 but phosphorylation of HNF-4 in the in vitro transcription system was observed.

203 The transcription system was purified extensively by DEAE-Se

204 A highly reconstituted RNA polymerase II transcription system was refractory to the effect impose

205 An in vitro transcription system was used to examine the contributio

206 Using a homologous in vitro transcription system, we demonstrate in this study that

207 In this study, by using an in vitro transcription system, we demonstrate that (i) only phosp

208 lly, using a fully recombinant mitochondrial transcription system, we demonstrate that MRPL12 stimula

209 ing a highly purified in vitro mitochondrial transcription system, we found that 1) the mtRNAP requir

210 Using a highly purified human transcription system, we found that chromatin, TAFs, and

211 Using an in vitro transcription system, we found that transcription effici

212 With an in vivo VSV minigenome transcription system, we further show that a deletion mu

213 By adapting the tetracycline-regulated gene transcription system, we generated a murine model where

214 Using an in vitro transcription system, we have discovered that, unlike mo

215 l nuclear extract (NE)-based in vitro runoff transcription system, we have examined the effect of Sp1

216 By using a purified in vitro transcription system, we have genetically dissected the

217 ere, using a fully native S. aureus in vitro transcription system, we provide the first molecular and

218 ng a highly purified, reconstituted in vitro transcription system, we show that HSF-4a represses basa

219 the M. jannaschii high-temperature in vitro transcription system, we show that Ptr2 is a potent tran

220 Using an in vitro transcription system, we show through factor competition

221 h-resolution and wholly recombinant archaeal transcription systems, we are beginning to understand th

222 een reconstituted S. pombe and S. cerevisiae transcription systems, we confirmed previous observation

223 Using reconstituted cell-free transcription systems, we found that cellular enhancer-b

224 uncapped viral +RNAs produced in an in vitro transcription system were determined, we found that the

225 Using an inducible transcription system which allows the regulated expressi

226 stablished the existence of a second plastid transcription system which does not utilize E.coli-like

227 We propose that the T7-like transcription system, which consists of a phage-specific

228 transactivation, we established an in vitro transcription system with chromatin templates, in which

229 the initiation properties of a mycobacterial transcription system with E. coli RNAP on two different

230 so be observed in this highly purified human transcription system with either mouse or yeast TBP, TAF

231 In a transcription system with highly purified components, we

232 Using a cell-free transcription system with nuclear extract from 3T3-L1 pr

233 nd Mastermind-like 1 (MAML1), in an in vitro transcription system with purified factors and naked DNA

234 Using an in vitro transcription system with purified factors and pol II la

235 An in vitro transcription system with purified human mitochondrial R

236 Here, we use a reconstituted in vitro transcription system with purified polymerase II (Pol II

237 Using an in vitro transcription system with purified RNA polymerase (RNAP)

238 (CPD) using enzymatic probes and an in vitro transcription system with purified RNA polymerase II (RN

239 Using an in vitro transcription system with purified T7 RNA polymerase (T7

240 lated transcription using a defined in vitro transcription system with RNA polymerase as the only pro

241 also found to be synthesized by an in vitro transcription system with the native VSV RNP.

242 The addition of purified H-NS to an in vitro transcription system yielded a fivefold or greater reduc