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1 KG1beta RQ variant isolated from transiently transfected 293T cells, indicating that increased basal
2 duced the infectivity of HIV-1 produced from transfected 293T cells, the Jurkat TAg T-cell line, and
5 n cassettes CMV- Zif-VP64 and CMV-eGFP-S/MAR transfected a)K562 cells; b)murine beta-YAC bone marrow
6 NA expression at the site of administration, transfecting a substantial proportion of tumor-infiltrat
9 produced the triploid vector AAV2/8/9 by co-transfecting AAV2, AAV8 and AAV9 helper plasmids at a ra
12 KO mice (1-, 2-, and 4 wk old) and Adeno-Cre-transfected ALR (flox/flox) hepatocytes were used for in
17 ocyte fractionation localized endogenous and transfected APOL1 using a large panel of novel APOL1-spe
19 roscopy revealed that nanoplexes efficiently transfect bacterial cells, consistently with biological
21 ed that the transcriptome of sonoselectively transfected brain endothelium was unaffected by the trea
22 ansfection and persisted for 3 days in cells transfected by pEF-GFP and pUB-GFP plasmids and for at l
24 visualize dual organelle dynamics in hard-to-transfect cell lines by super-resolution for over an ord
25 However, for complex samples such as mock transfected cell culture fermentation, best results were
28 , macrophages are recognized as difficult to transfect cells and non-specific delivery would inevitab
30 ificantly reduced VEGF expression in hard-to-transfect cells when compared with commercial platforms
34 ound to be internalized in both infected and transfected cells after the addition of either RSV-speci
35 ntrast was obtained in cell lysates of rdLRP transfected cells and in in vivo LRP expressing mouse br
37 strated that MDV U(S)3 interacts with Meq in transfected cells and MDV-infected chicken embryonic fib
38 ocessing factor CPSF6 interacted with NP1 in transfected cells and participated with NP1 to modulate
40 termed MGAT4D-L, inhibits MGAT1 activity in transfected cells and reduces the generation of complex
41 ransport of DA (i.e. anomalous DA efflux) in transfected cells and to drive hyperlocomotion in Drosop
42 expression of Rh05 results in IgG binding to transfected cells consistent with Rh05 being a vFcgammaR
43 a striking binucleated phenotype in miR-1300 transfected cells due to cytokinesis failure followed by
45 rt, recombinant VP7 protein overexpressed in transfected cells formed trimers only when cotransfected
46 by using in silico prediction and plasma and transfected cells from both patients and control subject
47 to the appearance of GFP-MuMx1 in 20-30% of transfected cells in a novel cytoplasmic giantin-based i
48 and Cas9 from a single expression vector in transfected cells in combination with multiple rounds of
49 9 K562 cells and its overexpression in ABCC4-transfected cells provided evidence that ABCC4 is the ge
50 ow that DENV-infected monocytes and DENV-NS1-transfected cells release high amounts of EVs loaded wit
52 ransgenic mice, FTLD-TDP patient brains, and transfected cells to assess the interplay between CHCHD1
53 cular fluorescence complementation assays in transfected cells to elucidate how altering cholesterol
54 ation of IFT43 in the retina and transiently transfected cells was examined by RT-PCR, western blot a
55 uclear localization of the mutant protein in transfected cells was significantly reduced compared to
56 SP6 positively modulates GluN1 expression in transfected cells, and USP6 down-regulation impedes foca
62 s of mouse astrotactin-2 (Astn2) produced in transfected cells, we determined that the amino and carb
64 35) decreased activation of hTLR4- and hTLR9-transfected cells, whereas PV from IRI- patients (n = 32
65 as observed in HSV-infected cells but not in transfected cells, which argued against direct US9-gE/gI
66 er confirms autonomous genome replication in transfected cells, with initiation of replication from t
89 alone and CREB plus HIPK2 over empty vector-transfected control displayed 14- and 32-fold upregulati
90 -/-) and (2) reduced the current in AtALMT12 transfected COS-7 cells, strongly suggesting the direct
91 nd p.Ala210Val SLC45A1 variants, measured in transfected COS-7 cells, was approximately 50% (p = 0.01
92 ediates cued cocaine seeking, we selectively transfected D1-MSNs in transgenic rats with an inhibitor
93 ter becoming near-tetraploid, we transiently transfected diploid cells with siRNA against ESPL1/Separ
95 emically administered autologous macrophages transfected ex vivo to produce glial cell line-derived n
96 (+)RNAs [(+)ssRNAs], a chimeric plasmid was transfected, from which the capping enzyme NP868R of Afr
99 rotein interaction, and adhesion of integrin-transfected HEK 293 cells, we identified a sequence (cal
100 With calcium mobilization assays of FPR3-transfected HEK 293 cells, we measured FPR3 signaling in
101 rose gradient centrifugation and transiently transfected HEK 293T cells to identify the species of al
102 binding to hippocampal neurons and to CASPR2-transfected HEK cells led to some internalization of the
103 nescence resonance energy transfer assays in transfected HEK cells showed that RGS6 prefers Galpha(o)
104 , Ca(2+) influx and YOPRO-1 uptake in stably transfected HEK-293 cells (HEK-hP2X7) plus enhanced cell
105 ys and patch clamp recordings of transiently transfected HEK-293 cells for electrophysiological chara
106 se activity were demonstrated in transiently transfected HEK293 cells comparing wild-type and mutant
107 tion in patient plasma and in supernatant of transfected HEK293 cells expressing recombinant W268R-mu
109 y characterized mouse and zebrafish TRPA1 in transfected HEK293 cells using calcium assays as well as
115 f enzyme activities in lysates prepared from transfected HEK293T cells expressing these enzymes mirro
116 ed in peripheral blood mononuclear cells and transfected HEK293T cells through immunoblotting, immuno
118 stics, plus their pathogenic potential using transfected HEK293T cells, rodent neuronal preparations,
122 tioned medium from Corin-siRNA- or ANP-siRNA-transfected HK-2 cells was determined by western blottin
123 rNTPs to a DNA primer, but the expression of transfected hpol eta in the cells restored this ability.
124 y binding and competition experiments on FAP-transfected HT-1080 (HT-1080-FAP) or on mouse FAP-expres
126 uman POSTN isoform 1 (hPOSTN-001), we stably transfected human chondrosarcoma 1 (hCh-1) cell line wit
127 h TLR2, as determined using a variety of TLR-transfected human embryonic 293 cell lines, while the PF
128 nts on fMLP-stimulated neutrophils and 5-LOX-transfected human embryonic kidney cells, propofol atten
130 efficacy of the CRISPR targets was tested by transfecting human embryonic kidney (HEK293) cells with
132 ge, while cytoplasmic MLOs formed in AfrLEA6-transfected insect cells behave like stress granules.
134 odes are counted by sequencing DNA libraries transfected into cells and the cell's output RNA in orde
137 tein abundance when each of these miRNAs was transfected into human breast cancer MDA-MB-231 cells.
140 variants resist conversion to PrP(res) when transfected into scrapie-infected N2a neuroblastoma cell
141 or wild-type and mutant WNT10A were made and transfected into stem cells from human exfoliated decidu
143 ructs encoding Kv4.3, KChIP1, and DPP10 were transfected into the injured DRGs (defined as DRGs with
144 n-regulation of SERINC5 from the membrane of transfected Jurkat cells by these mutants was significan
147 by control and OR10G7 small interfering RNA-transfected keratinocytes in response to odorant stimula
151 concentrations than control cells, and SKIP-transfected leukemia cell lines exhibited increased SK a
152 presentation assays and single HLA molecule-transfected lines, we experimentally determined HLA rest
153 ed KOR agonist-mediated activation of ROS in transfected live cells by two fluorescent sensors, CellR
156 we validated a therapeutic potential of GDNF-transfected macrophages in a transgenic Parkin Q311X(A)
158 Transwell studies in hOCT2/hMATE1 double-transfected Madin-Darby canine kidney cells showed that
159 he newly developed TetOn-inducible system in transfected male and female mouse cones in vivo, we show
163 Compared to control db/db mice, LepR(b) transfected mice showed significantly higher V(E) throug
164 ly abundant miRNAs on glucose metabolism, we transfected miR-20b-5p, a highly abundant exoRNA in pati
165 land miRNAs in POWV replication in-vitro, we transfected miRNA inhibitors into VeroE6 cells to profil
166 Using a novel TetOn-inducible system in transfected mouse cones, we showed that the LE pathway o
167 Here, we used Channelrhodopsin-2 (ChR2) transfected mouse embryonic stem cell (mESC) derived mot
168 lsed bone marrow-derived dendritic cells and transfected murine fibroblasts expressing antigenic MHC:
173 n and organization of rosettes in active Src-transfected NIH3T3 fibroblasts and cancer-associated fib
175 of Ran-GTP, which accumulates at nucleoli of transfected or infected cells, thus perturbing the nucle
177 of these truncating variants in transiently transfected orthologous cells and cultured hippocampal r
178 e, or membrane-blebbing function, and double-transfected P2X7L and P2X7A cells have reduced pore func
182 n the surface of the sporozoite, transiently transfected populations of E. tenella expressing the flu
183 different types of cells, including hard-to-transfect primary cells, with delivery efficiency up to
184 embryonic kidney cells (HEK293) and hard-to-transfect primary human umbilical vein endothelial cells
186 n RNA-silenced human MC line LAD2 and stably transfected RBL-2H3 cells, we demonstrate that AG-30/5C
187 accharide maturation as well as that of a co-transfected secretory membrane glycoprotein, vesicular s
189 ilar results were obtained using infected or transfected spleen cells or bone marrow-derived DCs.
190 hown that plasmid polyplexes can non-virally transfect SVZ NPCs when directly injected in the lateral
191 elial lung cells using a non-viral vector to transfect the telomerase catalytic subunit (hTERT) and t
192 g zebra finch PGCs in vitro and were able to transfect them with gene-expression vectors and incorpor
195 a, however, it has become common practice to transfect these cells with luciferase for fluorescent tu
196 h support entry and replication of HCV, were transfected these cells with small interfering RNAs targ
197 ter showed that inner hair cells (IHCs) were transfected throughout the cochlea, and outer hair cells
199 1) as exemplars of each class, in cell lines transfected to express either human or mouse GPR84, both
200 lves optogenetic stimulation of cells stably transfected to express light sensitive protein channels
202 -CAR and SKOV3-CAR cells (CHO or SKOV3 cells transfected to stably express human coxsackievirus and a
206 lopment of engineered VF mucosae from hiPSC, transfected via TALEN constructs for green fluorescent p
207 now achievable and it is possible to obtain transfected viable parasites under in vivo growing condi
208 proach to measure gene expression induced by transfected viral genomic RNA (vgRNA) and reovirus infec
209 of glycosylation-deficient Leu515Phe and co-transfected VSVG to the cell surface, however, was relat
211 combined electrophysiological recordings of transfected wild-type or mutant channels in both neurobl
212 lation spectroscopy (FCCS) of live HEK cells transfected with 2 spectrally different OLIG2 clones.
214 ed in human embryonic kidney (HEK)-293 cells transfected with a constitutively active YAP mutant, and
215 ive transfer of old CD4(+) T cells that were transfected with a lentiviral vector inducing constant e
216 ly suppressed luciferase expression in cells transfected with a luciferase reporter linked to the put
217 pressing a luciferase-based AR reporter were transfected with a miR inhibitor library; 78 inhibitors
229 EGFP expression was observed when MCF-7 were transfected with C(3)-ZIF(MCF) compared to 1-fold repres
232 end, Ogg1/OGG1 knockout/depleted cells were transfected with constructs expressing wild-type (wt) an
233 thermore, a focused gene array between cells transfected with CREB alone and CREB plus HIPK2 over emp
234 deficient (CRF(2)(-/-)) mice and RBL-2H3 MCs transfected with CRF(2)-overexpressing plasmid or CRF(2)
238 tures were transformed by ras oncogenes when transfected with DNA from neoplastic cells, but they fai
239 lls (lacking endogenous ENBT1 activity) were transfected with each of the coding region variants of S
241 nished upon the same treatment of A549 cells transfected with either p53 siRNA or acetylcholinesteras
242 blot analysis performed on (I) HEK293 stably transfected with empty vector compared to wild-type frat
243 n occur outside the germline within S2 cells transfected with eRpL22-like, indicating that germline-s
245 energy transfer experiments in HEK293 cells transfected with fluorescently labeled SERCA2a revealed
248 nt of stromal cells with TCM from PC-3 cells transfected with GPC-1 shRNA increased the expression of
249 talized keratinocytes (NIKS) and NIKS stably transfected with HPV16 episomal genomes (NIKS16) were co
250 ied in A431 human epidermoid carcinoma cells transfected with human CCK2R (A431-CCK2R), as well as th
251 ance energy transfer (FLIM-FRET) of cells co-transfected with huntingtin proteins containing short an
252 issue: 1) cultured healthy human macrophages transfected with hY3, an anti-SSA/Ro-associated ssRNA, 2
253 oxia-induced proangiogenic effect, CSCs were transfected with hypoxia-inducible factor-1 alpha (HIF-1
254 ndent wild-type (WT) ATL cells but not cells transfected with IL-2, suggesting that in IL-2-producing
256 ctivation of the channels when cells were co-transfected with Kv4.3 (or Kv4.2), KChIP2 and another be
257 Importantly, we show that leukaemia cells transfected with light-inducible NPs containing RA can e
258 ithelial cells (Caco-2 and IEC-6 lines) were transfected with LNA-anti-uc.173 or uc.173 transgene.
259 and spread was assessed in epithelial cells transfected with locked nucleotide analog antagonists (L
261 HD, including mouse and fly models, neurons transfected with mHTT, HD iPSC-derived neurons, and huma
262 and PBECs derived post-lung transplant were transfected with miR-200b-3p mimics and EMT marker expre
265 (IFN-gamma) in both infected cells and cells transfected with miR-H28, (ii) IFN-gamma accumulated con
268 dothelial, bEnd.3, Madin-Darby canine kidney transfected with multi-drug resistant protein 1 (MDCK-MD
271 igration of human airway smooth muscle cells transfected with PKAc variants containing amino acid sub
272 ng CRISPR/Cas9 gene editing; some cells were transfected with plasmids that express FAF1 missense var
275 euronal tissue, cancer cell lines, and cells transfected with recombinant CDR2 and CDR2L to elucidate
277 llular beta-catenin levels in MC3T3-E1 cells transfected with scrambled or protein kinase B (Akt) sma
281 er, incubated with LTB receptor agonist, and transfected with small interfering RNAs to knock down RE
283 were isolated from heterozygous patients and transfected with SNP-targeted siRNA, using glucan partic
288 assessed in 1321N1 astrocytoma cells stably transfected with the human P2X1 receptor by measuring in
291 ved from MDA-MB-231 breast cancer cells were transfected with the vinculin tension sensor to quantita
293 determined by Western blot in HEK293T cells transfected with variant or wild-type human SOS1 express
296 8 and CDK19 CRISPR double-knockout cell line transfected with wild-type (WT) or mutant CDK8 construct
297 udies performed on HEK293T cells transiently transfected with wild-type and mutant SLC13A3 indicated
298 ing, and immunofluorescence in NIH/3T3 cells transfected with wild-type and mutated human L1 genes.
299 s their sensitivity to TRAIL treatment; when transfected with wild-type Cosmc, these tumor cells thus