ライフサイエンスコーパス: transgene

1 he second mutating and inactivating the cas9 transgene.

2 n which human FcgammaRIIa was expressed as a transgene.

3 sm for human cells that is not mediated by a transgene.

4 on rates of keratinocytes not expressing the transgene.

5 pe of tectal neurons labeled by an id2b:gal4 transgene.

6 tificial chromosome (HAC) expressing the GFP transgene.

7 n comparison with mice harboring only the AR transgene.

8 rotein changes driven by the alpha-synuclein transgene.

9 ts antigenicity-the immunity elicited to the transgene.

10 f a tetracycline-regulatable (Tet-off) v-myc transgene.

11 esis by blocking the expression of the ErbB2 transgene.

12 ineered to express an antigen from a foreign transgene.

13 be inherited even following loss of the CMT3 transgene.

14 inducible tissue-specific expression of PG-1 transgene.

15 lentiviral vector delivery or induction of a transgene.

16 e tracheal AMP gene promoter-controlled PG-1 transgene.

17 es but currently rely on the introduction of transgenes.

18 ranscriptionally active viral and endogenous transgenes.

19 using CRISPR/Cas9 components expressed from transgenes.

20 uce potent immune responses against inserted transgenes.

21 to viruses, transposable elements, and some transgenes.

22 and GR dipeptide production from (G4C2)(30+) transgenes.

23 super-Mendelian inheritance of the separate transgenes.

24 sts expressing DMP1-Cherry and DSPP-Cerulean transgenes.

25 n in backgrounds containing kinetochore RNAi transgenes.

26 on of adeno-associated virus (AAV)-delivered transgenes, allowing dose-dependent and up to 223-fold r

27 worms harboring either the Abeta1-42 or tau transgene alone and interestingly without changes to the

28 We show that the Nix transgene alone, in the absence of the M-locus, was suff

29 Longer, spliced Xist transgenes also induced robust silencing yet terminated

30 GaRe doxycycline inducible SpCas9 (ODInCas9) transgene and its use in targeted ObLiGaRe results in fu

31 nerated mouse model in which both murine Met transgene and stabilized beta-catenin are conditionally

32 g developments in the incorporation of human transgenes and additional mutations in humanized mouse m

33 otypes, T-cell function, immune responses to transgenes and autoantibodies, vector copy number, and i

34 ithout or with SMAD7 (sCYLD/SMAD7 mice) from transgenes and CYLD-knockout mice (with or without trans

35 nd 3-7-fold higher 1-2 h after HS for HSPA1B transgenes and endogenous genes, respectively.

36 Here we show that HSPA1B BAC transgenes and endogenous Hsp70 genes turn on 2-4 min af

37 control would provide safe biocontainment of transgenes and gene drives.

38 y of complex VSV recombinants carrying large transgenes and support further clinical development of o

39 RNAs (mRNAs) of a green fluorescent protein transgene, and CD46, CD55 and CD71 cell-surface proteins

40 he tissues of P(1) individuals harboring the transgene, and high transgene expression was observed in

41 defects can be corrected by ASF1A and ASF1B transgenes, and that ASF1A and ASF1B act redundantly.

42 t transcript levels measured adjacent to the transgene are approximately twofold higher for speckle-a

43 Females with one copy of the FL3#2 transgene are viable but up to 99.8% of homozygous femal

44 Since the discovery two decades ago that transgenes are efficiently integrated into the genome of

45 kout (TKO) pigs (with added protective human transgenes) are likely to be optimal sources of organs f

46 sing CRISPR/Cas9 technology to integrate the transgene at the safe-harbor AAVS1 locus in DLD-1 cells.

47 Targeted insertion of transgenes at pre-determined plant genomic safe harbors

48 tionately expanded in mice expressing a Baff transgene (B6.BTg) and were disproportionately contracte

49 To bypass these barriers, we designed a transgene-based system in Drosophila that increases the

50 its broad tropism can be detrimental if the transgene being delivered is harmful when expressed ubiq

51 This is partially a result of the Pf4-Cre transgene being expressed in a variety of leukocyte popu

52 Transgene brain expression was sporadic at the cellular

53 tested the effectiveness of a single CRISPRi transgene broadly expressing a single guide RNA and a ca

54 is in C. elegans by engineering a multicolor transgene called NeuroPAL (a neuronal polychromatic atla

55 se results demonstrate that a single CRISPRi transgene can effectively suppress a target gene in mice

56 In the field, the WPGD1 and WPGD2 transgenes can mitigate grain yield losses in high-night

57 However, these human SMN missense allele transgenes can rescue a null Smn allele when SMN2 is pre

58 tial degree of freedom in the engineering of transgenes capable of simultaneously expressing multiple

59 and established two independent colonies of transgene carriers.

60 deliver an instability-prone Mecp2 (iMecp2) transgene cassette which, increasing RNA destabilization

61 V transduction efficiency (by optimizing the transgene cassette), vector tropism (using capsid engine

62 at inactivation of Rb plus p53 via a WAP-Cre transgene, commonly used to target the mammary gland dur

63 WPGD1 and WPGD2 transgenes complement the pgd3-defective kernel phenotyp

64 Introduction of a muscle-specific Chkb transgene completely rescues motor and behavioral functi

65 Critically, the same ThPOK transgene completely restored the CD4 helper lineage com

66 mouse embryonic stem cells, expression of a transgene comprising the first two kilobases of Xist (Xi

67 y active KRas (caKRas) allele and the CD4Cre transgene contain not only hyperactivated T cells but al

68 g a PTC in the mutated gene or introducing a transgene containing a PTC to trigger a GCR.

69 Out of 151 zygotes injected with circular transgene-containing plasmid and Cas9 protein loaded wit

70 Integrated transgene-containing transposons were quantified after l

71 ag2(-)/(-) model of ALS with lower number of transgene copies and longer lifespan.

72 CAR T cells were measurable by transgene copy PCR up to 10 months.

73 We demonstrate chloroplast-targeted transgene delivery and transient expression in mature Er

74 vascular BOLA3 was performed by orotracheal transgene delivery of adeno-associated virus in mouse mo

75 This nanoparticle-mediated chloroplast transgene delivery tool provides practical advantages ov

76 ctivation of the nervous system via systemic transgene delivery.

77 Moreover, the transgene-dependent luciferase activity showed a positiv

78 Transgene-derived human factor VIII (hFVIII) protein act

79 ), which can vary substantially depending on transgene design and delivery.

80 protein-coupled receptor kinase 2 (GRK2-DN) transgene diminishes AR and AR target gene expression in

81 We show that the transgenes disrupt the coding sequence of endogenous gen

82 Further, the GRK2-DN transgene dramatically accelerates oncogene-initiated pr

83 e line with a targeted-insertion of the same transgene driven by the same tetracycline-TransActivator

84 When I-PpoI is expressed from a transgene during spermatogenesis in mosquitoes, the pate

85 y a gene drive either to inactivate the cas9 transgene (e-CHACRs) or to delete and replace the gene d

86 y, while GRASLND overexpression - either via transgene ectopic expression or by endogenous activation

87 tin gene as a species control, and P35S as a transgene element found in many GMO varieties.

88 bstantially higher levels of the therapeutic transgene, enabling the use of lower and safer vector do

89 The (FSF)TGFbeta(CA) allele consists in a transgene encoding a constitutively active mutant form o

90 n live mice and colon tissues that express a transgene encoding the calcium indicator GCaMP, we visua

91 specific to the Ad4 serotype rather than the transgene expressed.

92 r are at least partially interchangeable, as transgenes expressing ALA6 in vegetative tissues partial

93 sured by observing the inheritance of linked transgenes expressing different colors of fluorescent pr

94 in a bidirectional manner for bioimaging of transgene-expressing PCs in zebrafish (both sexes) with

95 bnormalities in Mac(TRAP) mice and confirmed transgene expression across various organs.

96 lymphoid progenitors driven by synthetic CAR transgene expression and encourage further evaluation of

97 rage exhibited more uniform and higher-level transgene expression both in the 3D model and in vivo, w

98 ermissive chromatin structure has assembled, transgene expression can persist even in the absence of

99 juvenile mice results in loss of persistent transgene expression due to hepatocyte proliferation in

100 ides the longest term evidence of persistent transgene expression following gene delivery to the CNS

101 "toolkit" enables efficient transduction and transgene expression from neurons, microglia, astrocytes

102 enhanced delivery (CED), provides widespread transgene expression in heathy rodent striatum and an ag

103 n and that sustaining hypertranscription and transgene expression in hyperproliferative cells early i

104 wherein conditional NeuroD2-controlled REST transgene expression in lineage-committed Ptch1 (+/-) ce

105 r, which specifically and potently sustained transgene expression in mouse RGCs and also works in hum

106 ambient CO(2)) on exogenous Bt toxins and transgene expression in promoterregion and codingregion

107 D-PG, which provides robust transduction and transgene expression in response to p53.

108 of a high dose of GAd accomplished extensive transgene expression in the lung yet elicited no detecta

109 vector inactivation and causing a transient transgene expression in the target tissue.

110 transgene mehtylation in promoterregion with transgene expression is even stronger than that in codin

111 me editing applications because only minimal transgene expression is required for activity.

112 ressing asyn have previously been developed, transgene expression is usually driven by pan-neuronal p

113 ransfection efficiency enhancement and rapid transgene expression kinetics of GFP plasmids at no comp

114 godendrocytes, alone or in combination, with transgene expression lasting for many months.

115 and nuclear membrane quickly to promote the transgene expression level and kinetics in both adherent

116 ingregion were negatively correlated with Bt-transgene expression level.

117 High transgene expression mimicked gene deletion, with failur

118 Here, delayed transgene expression of TDP-43(A315T) by oral doxycyclin

119 lted in therapeutically inadequate levels of transgene expression or have raised safety concerns asso

120 region of transgene plays a critical role in transgene expression regulation and silencing of transge

121 mad7 bigenic (double transgenic) mice, Smad7 transgene expression reversed transforming growth factor

122 However, rigorous assessment of transgene expression specificity in these animal models

123 However, reproducibly targeting transgene expression to specific fractions of a genetica

124 Silencing of exogenous DNA can make transgene expression very inefficient.

125 ndividuals harboring the transgene, and high transgene expression was observed in the blood of some P

126 Transgene expression was regulated by the pathogen-induc

127 completed in a few minutes and allows stable transgene expression within a few days, at success rates

128 knockout mice, was found in regions of high transgene expression within the cerebellum.

129 Of note, the transgene expression within the orthotopic tumor tissue

130 itches with wide dynamic ranges that control transgene expression without the requirement of addition

131 shows exceptional cellular internalization, transgene expression, and low cytotoxicity compared to c

132 in vitro and in vivo assays with a focus on transgene expression, cell loss, and neuroinflammatory r

133 richment of recombinant cells independent of transgene expression, permitting the assessment of libra

134 and endogenous protein detection, along with transgene expression, the less abundant BRD4 short isofo

135 r development was prevented by forcing PDE7B transgene expression, while knockdown of PDE7B effective

136 Two mutations in nsP2 and nsP3 enhanced transgene expression, while three mutations in nsP3 regu

137 triggered by viral vectors often suppresses transgene expression.

138 ion, intravenous delivery, and evaluation of transgene expression.

139 vity-dependent and spatiotemporally resolved transgene expression.

140 estoration of cell viability with a Bcl-x(L) transgene failed to rescue B cell development in Fnip1-d

141 expressing the Caenorhabditis elegans Fat-1 transgene (Fat-1(tg)xApoe(-/-)), which enables the endog

142 homing, incomplete penetrance of toxins and transgene fitness costs, each of which are of practical

143 Rescue from a different ClvR, they spread to transgene fixation in populations fixed for the latter a

144 Finally, ClvR (tko) spreads to transgene fixation.

145 integrates into a single, all-in-one vector transgenes for Cas9, sgRNA, and a fluorescence marker.

146 ic combinatorial co-transformation (up to 20 transgenes) for increasing C and N flows with the purpos

147 ometrically optimized DNA nanostructures for transgene-free and force-independent siRNA delivery and

148 ed SCAP-O and subclone SCAP-O(BCOR-mut) into transgene-free iPSCs using an excisable lentiviral vecto

149 ions were transmitted to at least 60% of the transgene-free T1 plants, with 33% of them containing bi

150 nd one of them, ClvR (n+1), carries a Rescue transgene from an earlier element, ClvR (n) ClvR (n+1) s

151 ish HSCs by a combination of two HSC-related transgenes, gata2a:GFP and runx1:mCherry.

152 in mice lacking expression with and without transgenes generating amyloid-beta (Abeta) plaque pathol

153 elevated levels of GFAP from a human WT GFAP transgene has contributed to the notion that the mutatio

154 . aegypti mosquitoes harboring the anti-ZIKV transgene have significantly reduced viral infection, di

155 o genetic load resulting from the integrated transgenes impaired drive performance in the trials.

156 y of complex VSV recombinants carrying large transgenes.IMPORTANCE Vesicular stomatitis virus (VSV)-b

157 tion and gene therapies via AAV-delivered F8 transgene in an episome are costly and nonpermanent.

158 one by inserting the human lens alphaAN101D transgene in CRYalphaA(N101D) mice, and in the other by

159 he other by inserting human wild-type alphaA-transgene in CRYalphaA(WT) mice.

160 espite the correct expression of TGFbeta(CA) transgene in fibroblasts.

161 , we tested whether expressing a therapeutic transgene in leukocyte progenitors that invade muscle wo

162 Supplying the MECP2 transgene in Mecp2R294X mice rescued phenotypic abnormal

163 ably, neuronal expression of IkappaBalpha-SR transgene in mice expressing TDP-43(A315T) or TDP-43(G34

164 Ectopic expression of a Lin28b transgene in murine B cells restored the positive select

165 s in siRNAs derived from a PRSV coat protein transgene in the absence of virus replication showed the

166 io distribution and expression levels of the transgene in the recipient post-transplant.

167 utive expression of a miR159-resistant GAMYB transgene in tobacco resulted in phenotypes similar to t

168 we observed a strong immune response to the transgene in treated male Mecp2 mutant mice that was ove

169 We designed a transgene in which leukemia inhibitory factor (LIF) is u

170 the p53 or eqFP650 portions of virus-carried transgenes in any of the passaged viruses, demonstrating

171 or mutations were found in the virus-carried transgenes in any of the passaged viruses.

172 etic aptazyme for control of nuclear-encoded transgenes in Arabidopsis (Arabidopsis thaliana).

173 rfactant resulted in strong silencing of GFP transgenes in both species.

174 /5 mutant phenotypes, and expression of ALA4 transgenes in pollen fully rescued ala6/7 mutant fertili

175 hat can be applied to constitutively express transgenes in specific cell types to examine their biolo

176 h allow for selection of multiple "unlinked" transgenes in the context of lentivirus-mediated transge

177 s essential for silencing different types of transgenes in the germ line and for suppressing the expr

178 umber of unexpected sequences in some of the transgenes, including undocumented cassettes and contami

179 y H2B-FP expression, occurring in all H2B-FP transgenes independent of label induction, accumulated w

180 ersed transforming growth factor (TGF)-beta1 transgene-induced inflammation, fibrosis, and subsequent

181 o increasing cardiomyocyte glucose uptake by transgene induction.

182 dly developed lung adenocarcinomas following transgene induction.

183 type seen in rTg4510 requires a ~70-copy tau-transgene insertion in a 244 kb deletion in Fgf14, a ~7-

184 in a 244 kb deletion in Fgf14, a ~7-copy tTA-transgene insertion in a 508 kb deletion that disrupts a

185 we report the first large-scale analysis of transgene insertion sites from 40 highly used transgenic

186 ut ready to go, cluster of P-element derived transgene insertions in Drosophila melanogaster, we show

187 The alphaDKRC transgene integrated between MYH6 and MYH7 and did not d

188 livery and strong protein expression without transgene integration is accomplished in Nicotiana benth

189 C57BL/6 founder, suggesting that the UBC-GFP transgene integration site is closely linked to the MHC

190 ts the importance and urgency of mapping the transgene integration site of transgenic mouse strains u

191 ols has been proven by the identification of transgene integration sites and flanking sequences in th

192 assive delivery of genetic material, without transgene integration, into plant cells for diverse biot

193 ng in high protein expression levels without transgene integration.

194 SLE, we introduced the FcgammaRIIA (FCGR2A) transgene into the NZB/NZWF1 mouse model of SLE.

195 tectal neuron type labeled by the id2b:gal4 transgene is a projection neuron that forms a stratified

196 ting transgenic mice where expression of the transgene is directed to these cells using the Eu promot

197 om the perspective of viral replication, the transgene is not only dispensable but may even be detrim

198 sue expression was observed with the Pf4-Cre transgene, leading to recombination in many hematopoieti

199 cient protein translation of the viral Mecp2 transgene, limits supraphysiological Mecp2 protein level

200 mbination labeling, inducible and reversible transgene manipulation, VCre recombinase expression, and

201 ine revertants that delete or inactivate the transgene may evolve to dominate the vaccine virus popul

202 ndogenous MCUb with a dominant-negative MCUb transgene (MCUb(W246R/V251E)) in vivo rescued T2D cardio

203 Transgene-mediated overexpression of alpha7beta1D integr

204 to 'maleness'-XX embryos could be rescued by transgene-mediated sex reversal or testosterone administ

205 The correlation of transgene mehtylation in promoterregion with transgene e

206 P2 + P3) at 1 N level, and it also increased transgene methylation levels in codingregion (P2), and i

207 ound biomass, total soluble protein content, transgene methylation levels in promoterregion (P1), and

208 The transgene methylation levels in promoterregion and codin

209 ion through the detection of AAV genomes and transgene mRNA, and show that intracellular and transmem

210 of a PTC and the nucleotide sequence of the transgene mRNA, which is homologous to the compensatory

211 pression of a synthetic, cancer-specific TCR transgene (NY-ESO-1).

212 Delivering the transgene of interest to target cells at levels high eno

213 onent configuration allows for combinatorial transgene optimization and increases safety by restricti

214 HBV (genotype D, serotype ayw)-either from a transgene or after infection with an adeno-associated vi

215 s that focus on a single receptor (using TCR transgenes) or a single specificity (using peptide-MHC t

216 Genetic manipulation via transgene overexpression, RNAi, or Cas9-based methods is

217 upts another five genes, in addition to high transgene overexpression.

218 n promoterregion but also in codingregion of transgene plays a critical role in transgene expression

219 ion in B6 mice homozygous for the PON1 human transgene [PON1Tg], PON1 knock-out mice [PON1KO], and wi

220 In vitro experiments in transgene positive alpha-cells demonstrated that EGFP ex

221 The present study crossed a SynCav1 transgene-positive (SynCav1(+)) mouse with the mutant hu

222 , efficient and fast whole-brain delivery of transgenes presents a persistent experimental challenge

223 The CD68.hMcl-1 transgene prevented mitochondrial reactive oxygen specie

224 Transgene-produced and recombinant FVIII-SQ showed compa

225 er experiments in 2 participants showed that transgene-produced FVIII-SQ accelerated early factor Xa

226 Surprisingly, the activity of transgene-produced FVIII-SQ was between 1.3 and 2.0 time

227 Higher OS activity for transgene-produced FVIII-SQ was observed across various

228 Segregation of the RNAi transgene produces non-genetic msh1 'memory' with multi-

229 Neo co-infected cells were positive for both transgene products.

230 s induced in newborn mice homozygous for the transgene Prom1(cre-ert2-nlacz) , which was knocked in t

231 NA transposons are more efficient with large transgenes, random integrations are potentially mutageni

232 We confirmed that the SNCA-A30P transgene recapitulates endogenous alpha-syn expression

233 Viral RNA replication from an endogenous transgene replicon system was not affected by lack of HI

234 ble for Orsay replication from an endogenous transgene replicon, suggesting that DRL-1 affects a prer

235 ect the TASOR and MPP8 domains necessary for transgene repression.

236 intersectional approach that involves three transgenes, requiring the intersection of two promoter/e

237 Tbx1(Cre) -activated expression of a Vegfr3 transgene rescued partially the cardiac lymphatic abnorm

238 Moreover, expression of Pnky from a BAC transgene rescues the differential gene expression and i

239 silence transcription, whereas a 5.5-kb Xist transgene robustly silenced transcription and read throu

240 A scheme based on genetic crosses and transgene selection was used to bypass F1 hybrid male st

241 e show that multiple DNA elements within the transgene sequences, including a metal response element

242 el alga Chlamydomonas have demonstrated that transgene silencing can be overcome by mutations in unkn

243 search has emphasized the risks of increased transgene silencing of Bacillus thuringiensis (Bt) rice

244 ly used reporter vectors and induces partial transgene silencing via the canonical and antiviral RNAi

245 Despite pervasive transgene silencing, technological advances have allowed

246 their application owing to immunogenicity or transgene silencing.

247 sable elements and the inability to initiate transgene silencing.

248 r evidence of either clonal dysregulation or transgene silencing.

249 injected systemically to deliver therapeutic transgenes site-specifically to diseased cells by respon

250 gy-directed genome engineering is limited by transgene size.

251 d an antigen from adenoviruses increased the transgene-specific CD8+ T cell responses in mice.

252 ctors typically use a B-domain-deleted FVIII transgene, such as human FVIII-SQ in valoctocogene roxap

253 pffer cells did not express the CSF1R-mApple transgene, suggesting that additional CSF1R transcriptio

254 pic expression of mouse or human Firre/FIRRE transgenes, supporting conserved trans-acting roles.

255 SIX proteins in host immunity, a human SIX1 transgene suppressed inflammation and promoted the recov

256 was observed as early as 4 weeks of age, and transgene suppression for the first 4 or 12 weeks of lif

257 and 3) T cells from mice that contain a CSK transgene susceptible to chemical inhibition.

258 -based, high-efficiency genetic pipeline for transgene swapping.

259 Using a dual transgene system for controlled expression at postimplan

260 This combinatorial study of multiple transgenes targeting primary metabolism thus offers oppo

261 dent of BAFF because of expression of a Bcl2 transgene) than in B6 wild-type mice despite the lower s

262 that could produce TIM proteins only from a transgene that cannot form the thermosensitive splicing

263 tation introduced into PHIL, a strain with a transgene that directs lineage-specific eosinophil ablat

264 Purification (TRAP)- approach and designed a transgene that expresses an eGFP-tagged ribosomal protei

265 three xenoantigens and to express nine human transgenes that enhance the pigs' immunological compatib

266 n, permitting the assessment of libraries of transgenes that perturb cell growth and survival.

267 A majority of transgenes that span a full topologically associating do

268 The potential bio-confinement of transgenes, the high protein expression and the possibil

269 gineering) and the ability of the capsid and transgene to avoid the host immune response (by genetica

270 n, or yellow emission channels, allowing the transgene to be used with numerous reporters of gene exp

271 lls is reversible, we developed an inducible transgene to restore expression of DNMT3A in transplante

272 Using a transgene to restore mss function to the androdioecious

273 ediated PCR, we successfully map the UBC-GFP transgene to the MHC locus.

274 ble and fluorescent dCas9-KRAB and dCas9-VPR transgenes to allow for accurate quantification and trac

275 , as well as insertion of "protective" human transgenes to counter the human immune response.

276 TIM4 mAbs in combination with CSF1R reporter transgenes to dissect the function of TIM4 in the chick

277 ucts such as plasmids, transposons, or other transgenes underlies many functional genomics measuremen

278 a Tom70 coding region with a Kozak-mini-GAL4 transgene using CRISPR-Cas9.

279 analysis revealed that copy number of hSOD1 transgene varied in our colony (4-8 copies).

280 in a single cassette, followed by removal of transgenes via Cre-mediated excision.

281 Overexpression of the human PON1 transgene was associated with reduced inflammatory arthr

282 A tandem alphaDKRC transgene was designed, validated in cultured cells, and

283 ulp during tooth development, the BSP-GFPtpz transgene was detected during in vitro mineralization of

284 onal degeneration so far; likely because the transgene was expressed in heterologous cell types.

285 was revealed when a B-cell antigen receptor transgene was found to circumvent the abnormal B-cell de

286 The encoded tdTomato transgene was highly expressed in all tissues evaluated.

287 In bones, the transgene was highly expressed in osteoblasts at an earl

288 Brain expression of the isoform 1 transgene was more abundant in hindbrain than forebrain

289 The PG-1 transgene was specifically expressed in the respiratory

290 ockin and activation of tTA-driven responder transgenes was tested using four transgenic lines that e

291 By analysing six uniquely designed transgenes, we demonstrate that the GCR is dependent on

292 To control expression of transgenes, we developed a miRNA regulation system that

293 specific tamoxifen-inducible Cre recombinase transgenes were used to target glioblastoma-relevant tum

294 we have developed a series of novel UAS-Cas9 transgenes, which allow fine tuning of Cas9 expression t

295 1 and Bxb1 integrase attP sites flanking the transgene will also enable rapid directional insertion o

296 In mice expressing a C9orf72 transgene with 450 repeats that did not encode the C9ORF

297 EGI couples a dominant lethal transgene with a recessive resistance allele.

298 ndently identified the presence of the HIV-1 transgene with at least 78.5% accuracy.

299 We used SMC- and EC-specific Cre recombinase transgenes with a novel floxed Eln allele to focus gene

300 so enable rapid directional insertion of any transgene without a size limitation at the ROSA26 locus.