ライフサイエンスコーパス: transgene expression

1 vels and reducing risks of heritable loss of transgene expression.

2 and 1 could also enhance vector-independent transgene expression.

3 ile effectively mediating gene silencing and transgene expression.

4 materialise in large part due to inefficient transgene expression.

5 crops used strong constitutive promoters for transgene expression.

6 ne expression in addition to more persistent transgene expression.

7 plastid transformation vector for inducible transgene expression.

8 neurons and are capable of stable, long-term transgene expression.

9 inclusion of an intron was also required for transgene expression.

10 ansferase (UFGT), which was dependent of the transgene expression.

11 ition from 20 weeks and mice with continuous transgene expression.

12 TH antisera was used to corroborate targeted transgene expression.

13 ter intron loss caused a 4-fold reduction in transgene expression.

14 sociated chromatin locus to activate in situ transgene expression.

15 mized expression constructs and strains help transgene expression.

16 polyplex but contrastingly greater levels of transgene expression.

17 This, in turn, initiates calcium-dependent transgene expression.

18 named pH11, which enables stable and robust transgene expression.

19 onfirmed the enhancement of polymer-mediated transgene expression.

20 adult mice, despite optimal and long-lasting transgene expression.

21 on of cultured cells and supported transient transgene expression.

22 examine mucosal lentiviral vector uptake and transgene expression.

23 triggered by viral vectors often suppresses transgene expression.

24 h epigenetic variation and/or instability of transgene expression.

25 robust capacity for retrograde infection and transgene expression.

26 h activation of GFP expression indicates Cre transgene expression.

27 ation of novel epialleles, and regulation of transgene expression.

28 1BS element mutations resulted in variegated transgene expression.

29 e molecular mechanisms underlying successful transgene expression.

30 ion, intravenous delivery, and evaluation of transgene expression.

31 rprisingly sensitive reporters of off-target transgene expression.

32 ce of versican respond with an activation of transgene expression.

33 blasts into integration-free iCMs via robust transgene expression.

34 from methylation, thereby conferring stable transgene expression.

35 or the ability to drive heterologous nuclear transgene expression.

36 vity-dependent and spatiotemporally resolved transgene expression.

37 tein isoforms, relying instead on cDNA-based transgene expression.

38 evious work demonstrated that SC-Ads amplify transgene expression 100-fold and produce markedly stron

39 , characterized by a robust induction of the transgene expression (17.7- to 73-fold) in the presence

40 y the most highly preferred codons decreased transgene expression (77- to 111-fold) when compared wit

41 bnormalities in Mac(TRAP) mice and confirmed transgene expression across various organs.

42 ted from preferential promoter usage because transgene expression after intravenous scAAV9/MeCP2-GFP

43 Irrespective of sex or transgene expression, ageing consistently reduced the se

44 Doxycycline administration can turn off the transgene expression allowing the restoration of Rai1 no

45 CIAO vectors provide reliable and targeted transgene expression and are extremely useful for recomb

46 mutant algal strains that permit high-level transgene expression and by determining the contribution

47 INPACT system provides exquisite control of transgene expression and can be adapted to potentially t

48 have developed a system to achieve sustained transgene expression and ECM formation by hMSCs.

49 lymphoid progenitors driven by synthetic CAR transgene expression and encourage further evaluation of

50 state in the liver consistent with sustained transgene expression and hepatic PAH enzyme activity wit

51 a T regulatory response that allows ongoing transgene expression and indicates that immunomodulatory

52 nomes is random, which often causes variable transgene expression and insertional mutagenesis.

53 he phenotype can be reverted by interrupting transgene expression and is EGFR dependent, as gland siz

54 o be less degraded by proteasomes, preserved transgene expression and largely avoided hepatotoxicity.

55 We evaluated the stability of transgene expression and long-term safety in 10 patients

56 tigated the biomass, exogenous Bt toxins, Bt-transgene expression and methylation status in Bt rice e

57 ences (CDSs) with higher GC content improved transgene expression and resulted in a remarkable trans-

58 These mice show ubiquitous transgene expression and TDP-43 knockdown in both the pe

59 choice of transcriptional promoters to drive transgene expression and the best ways to sink existing

60 ansion, oncogenic transformation, variegated transgene expression and transcriptional silencing.

61 thylation patterns that may adversely affect transgene expression and vector stability in vivo.

62 ) are known to allow robust and reproducible transgene expression and were targeted for integration o

63 of integrated T-DNA molecules likely affect transgene expression, and control of integration is cons

64 shows exceptional cellular internalization, transgene expression, and low cytotoxicity compared to c

65 of undefined T-cell mixtures, variability of transgene expression, and terminal differentiation of ce

66 -DZ cells results in a similar activation of transgene expression, and treatment with dasatinib, an i

67 is, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and

68 nce of nonintegrating vectors, and sustained transgene expression are among the major challenges in g

69 High levels of transgene expression are key to the success of improving

70 mutant algal strains that permit high-level transgene expression are less susceptible to epigenetic

71 vivo and determined that treatment initiated transgene expression as early as 2 days posttransduction

72 gue T5660136 significantly reduced Ad5 liver transgene expression at 48 h post-intravenous administra

73 e therapy are inefficient due to short-lived transgene expression attributed to the cytosine-phosphat

74 Surprisingly, we found that turning off the transgene expression before the onset of the phenotypes

75 ninvasive imaging of MSC biodistribution and transgene expression before therapeutic radioiodine appl

76 tudy using an AAV2/8 vector in dogs reported transgene expression beyond the boundary of the subretin

77 d AAV (ssAAV) vectors significantly augments transgene expression both in human cell lines in vitro a

78 rage exhibited more uniform and higher-level transgene expression both in the 3D model and in vivo, w

79 ected target tumor cells in an RD manner for transgene expression but that could be "switched" into a

80 ) model of hemophilia A, we increased cFVIII transgene expression by at least 100-fold with the use o

81 at the cationic RC5K can induce even greater transgene expression by increasing payload capacity with

82 ngs led to greater than 10-fold increases in transgene expression by multiple target cell types when

83 transgene expression would improve long-term transgene expression by using an engineered piggyBac tra

84 wn that reliability of the A2UCOE in driving transgene expression can be attributed to its resistance

85 cing via RNAe is irreversible, we found that transgene expression can be restored when hemizygous tra

86 Our results show that CAPN3 transgene expression can be successfully suppressed in t

87 ermissive chromatin structure has assembled, transgene expression can persist even in the absence of

88 Transgene expression, capillary density, graft function,

89 oses required to reach therapeutic levels of transgene expression caused liver inflammation in some p

90 in vitro and in vivo assays with a focus on transgene expression, cell loss, and neuroinflammatory r

91 frequency (PRF) for 2min exhibited superior transgene expression compared to all other treatment gro

92 circle DNA give enhanced and more persistent transgene expression compared to plasmid DNA in a number

93 We observed transgene expression confined to the renal cortex (speci

94 These results indicate that long term transgene expression consistency and T-DNA insert stabil

95 Long term transgene expression consistency and T-DNA insert stabil

96 s study addresses T-DNA insert stability and transgene expression consistency in multiple cycles of f

97 s study addresses T-DNA insert stability and transgene expression consistency in multiple cycles of f

98 The Tet-On/Off system for conditional transgene expression constitutes state-of-the-art techno

99 We found that repression of early transgene expression could prevent the priming of lucife

100 d RBPJ DNA binding ablates all Dll4 enhancer-transgene expression despite the presence of multiple fu

101 H-driven medulloblastoma, animals with Atoh1 transgene expression developed highly penetrant medullob

102 Moreover, the transgene expression dissipated after healing was comple

103 juvenile mice results in loss of persistent transgene expression due to hepatocyte proliferation in

104 ome is able to persist and provide prolonged transgene expression during in vitro differentiation of

105 AVrh32.33 vectors could establish persistent transgene expression, evade immunity, and minimize infil

106 However, transgene expression falls rapidly postdelivery due to a

107 ides the longest term evidence of persistent transgene expression following gene delivery to the CNS

108 flanking sequences that can be used to drive transgene expression for any organism where transcriptom

109 ., PLK1) can significantly enhance non-viral transgene expression for applications in biotechnology a

110 rs were non-cytotoxic and provided sustained transgene expression for at least three weeks in vitro.

111 Site-specific gene addition can allow stable transgene expression for gene therapy.

112 hydrogel in vivo led to a sustained level of transgene expression for more than two months while mini

113 ry region as important elements for vigorous transgene expression from a vector that is functionally

114 ich is transcriptionally inactive, efficient transgene expression from AAV vectors is dependent upon

115 roach sensitively captures both high and low transgene expression from AAV vectors.

116 -repeat enhancers have proven safe; however, transgene expression from cellular promoters is often in

117 100 rounds of cell division showed sustained transgene expression from episomes and provided molecula

118 "toolkit" enables efficient transduction and transgene expression from neurons, microglia, astrocytes

119 Suppression of therapeutic transgene expression from retroviral gene therapy vector

120 s is greatly hampered by the inefficiency of transgene expression from the nuclear genome.

121 .3- to 8-fold or 91- to 125-fold increase of transgene expression from the same batch of materials, d

122 and histone H3K4me3 patterns correlated with transgene expression; H3K9me3 marks partially correlated

123 We directed transgene expression in a fraction of HSCs that maintain

124 e versican G1 in enhancing adenoviral vector transgene expression in a hyaluronic acid-CD44 independe

125 mouse lungs resulted in a 6-fold increase in transgene expression in addition to more persistent tran

126 neurons, whereas scAAV9/beta-actin-GFP drove transgene expression in astrocytes.

127 ue-specific enhancers enables the control of transgene expression in both space and time.

128 nsgenic mouse lines that differ in levels of transgene expression in brain and periphery (APP23 mice,

129 The LV/CMV/ZGFP exhibited prolonged transgene expression in CHO cells and HSCs up to 10 days

130 0 kb of genomic 5'-flanking sequence, showed transgene expression in CNS oligodendrocytes from larval

131 ulated adeno-associated virus (AAV)-vectored transgene expression in cultured mammalian cells and mic

132 dorsal root crush and adeno-associated virus transgene expression in dorsal root ganglia.

133 how that myostatin inhibition by follistatin transgene expression in Dysf(-/-) mice results in early

134 neurons, whereas scAAV9/beta-actin-GFP drove transgene expression in GFAP(+) astrocytes.

135 enhanced delivery (CED), provides widespread transgene expression in heathy rodent striatum and an ag

136 Transgene expression in hPSCs is tightly regulated in re

137 n and that sustaining hypertranscription and transgene expression in hyperproliferative cells early i

138 Flcn H255Y mutant transgene expression in kidney-targeted Flcn knockout mi

139 wherein conditional NeuroD2-controlled REST transgene expression in lineage-committed Ptch1 (+/-) ce

140 13 deficiency, combined with conditional MYC transgene expression in mouse GC B cells, promotes lymph

141 r, which specifically and potently sustained transgene expression in mouse RGCs and also works in hum

142 igh-level stable gene transfer and sustained transgene expression in multiple primary human somatic c

143 ctive herpes simplex virus (HSV) vectors for transgene expression in nonneuronal cells in the absence

144 Efficient replication and transgene expression in normal human endothelial cells i

145 ted Nyx(nob) retina, robust and targeted Nyx transgene expression in ON BCs partially restored the ER

146 Megakaryocyte-specific transgene expression in patient-derived induced pluripot

147 as of the hepatic lobule, while there was no transgene expression in periportal areas.

148 ficiency germline transgenesis and sustained transgene expression in pigs by using the Sleeping Beaut

149 rovides both activation and amplification of transgene expression in planta.

150 ambient CO(2)) on exogenous Bt toxins and transgene expression in promoterregion and codingregion

151 ficiency germline transgenesis and sustained transgene expression in rabbits by using the Sleeping Be

152 D-PG, which provides robust transduction and transgene expression in response to p53.

153 ve outpatient procedure to obtain widespread transgene expression in retina and RPE.

154 it allows combinatorial cloning for multiple transgene expression in seeds, vegetative organs, or bot

155 tively and specifically drives Cre-dependent transgene expression in selected postsynaptic neuronal t

156 ivation of NMD cell autonomously knocks down transgene expression in specific cell types without affe

157 rged as a widely used platform for achieving transgene expression in specified neural populations.

158 d drove the highest level and specificity of transgene expression in stems.

159 Apart from the retina, we found transgene expression in subsets of periventricular neuro

160 ng in minimal cellular infiltrate and stable transgene expression in target muscles.

161 uronal-specific promoters were able to drive transgene expression in TG neurons.

162 e that lentiviral vector-mediated GT induces transgene expression in the B-cell compartment, resultin

163 simply genetically manipulated for efficient transgene expression in the differentiated cells, surmou

164 ossibility to drive efficient and persistent transgene expression in the heart offers the possibility

165 ic injection of different rat brain regions; transgene expression in the hippocampus lasted up to 6 m

166 ered CELiD DNA resulted in long-term, stable transgene expression in the liver.

167 of a high dose of GAd accomplished extensive transgene expression in the lung yet elicited no detecta

168 ific T cells in vivo versus the stability of transgene expression in the muscle, we confirmed that th

169 and mediate localized, robust, and sustained transgene expression in the rat brain.

170 vector inactivation and causing a transient transgene expression in the target tissue.

171 Transgene expression in these cells, however, can be sta

172 Transgene expression in tissues from these mice correlat

173 enous Bacillus thuringiensis (Bt) toxins and transgene expression in transgenic rice under different

174 CPL4, the overexpression of which inhibited transgene expression in transient assays.

175 ose-dependent endothelial cell infection and transgene expression in tumor biopsies of diverse histol

176 ficiency germline transgenesis and sustained transgene expression in two important biomedical models,

177 d, tissue-specific and copy-number dependent transgene expression in vitro and in vivo.

178 viding cell population and provide sustained transgene expression in vitro and in vivo.

179 Subsequently, transgene expression in vitro in RAW 264.7 cells and ex

180 e inability to achieve sustained, high-level transgene expression in vivo presents a significant hurd

181 as associated with markedly higher levels of transgene expression in vivo, particularly within draini

182 ression in most cell types and low levels of transgene expression in vivo.

183 Chlamydomonas RPL23 sequences also enabled transgene expression in Volvox carteri.

184 bled highly specific, long-term AAV-mediated transgene expression in white or brown adipocytes.

185 an inducible system to control the timing of transgene expression in zebrafish and chick.

186 dated in functional rescue experiments using transgenes expression in EPCs from retroviral vectors.

187 efforts have been made in refining mosquito transgene expression, in particular attenuating the effe

188 otor phenotypes correlate with levels of FUS transgene expression, indicating that toxicity in develo

189 man neurodegenerative disease, whereas Tau35 transgene expression is equivalent to less than 10% of t

190 transgene mehtylation in promoterregion with transgene expression is even stronger than that in codin

191 However, transgene expression is not amenable for therapeutic app

192 ply, while, to some extent, the exogenous Bt-transgene expression is reduced at sub-N levels (1/4 and

193 me editing applications because only minimal transgene expression is required for activity.

194 elerated degeneration induced by follistatin transgene expression is specific to mice lacking dysferl

195 ressing asyn have previously been developed, transgene expression is usually driven by pan-neuronal p

196 r mRNA therapeutics when predictable in vivo transgene expression kinetics is imperative.

197 ransfection efficiency enhancement and rapid transgene expression kinetics of GFP plasmids at no comp

198 Transfection efficiencies and transgene expression kinetics of messenger RNA (mRNA), a

199 Strikingly, discontinuation of transgene expression largely restored differentiation pr

200 godendrocytes, alone or in combination, with transgene expression lasting for many months.

201 PsGA3ox1 transgene expression led to higher GA1 concentrations in

202 and nuclear membrane quickly to promote the transgene expression level and kinetics in both adherent

203 ingregion were negatively correlated with Bt-transgene expression level.

204 nts in a system in which genetic background, transgene expression levels and post-translational prote

205 , providing design strategies for increasing transgene expression levels and reducing risks of herita

206 At transgene expression levels comparable to endogenous CtB

207 Non-viral vectors often yield transgene expression levels lower than viral counterpart

208 By assessing the correlation of transgene expression levels with isoprenoid marker metab

209 iple promoters and terminators, and variable transgene expression levels.

210 Use of megakaryocytes/platelets for transgene expression may take advantage of their rapid t

211 High transgene expression mimicked gene deletion, with failur

212 ecal space surrounding the spinal cord, with transgene expression observed within multiple cell types

213 studies with cell type-specific postsynaptic transgene expression of designer receptors activated by

214 We show that transgene expression of Dm-dNK in Tk2 knock-out (Tk2(-/-

215 n phenotype and the lethality are rescued by transgene expression of FATP4 in suprabasal keratinocyte

216 We therefore exploited the power of targeted transgene expression of mutant hDAT in Drosophila to exp

217 Here, delayed transgene expression of TDP-43(A315T) by oral doxycyclin

218 cellular promoters result in relatively low transgene expression, often leading to inadequate diseas

219 rough the BBB with FUS led to dose-dependent transgene expression only in the FUS-treated region that

220 editing technologies and tight regulation of transgene expression opens new doors for engineering.

221 hifts, allowing for cell-state and cell-type transgene expression optimization through codon content

222 lted in therapeutically inadequate levels of transgene expression or have raised safety concerns asso

223 In mice, fibroblast-specific Il11 transgene expression or Il-11 injection causes heart and

224 Re-expression of HEXIM1 (through transgene expression or localized delivery of a small mo

225 RGD loop had little to no effect on in vivo transgene expression or transgene-specific adaptive immu

226 establishing simple principles of successful transgene expression, our data open up new possibilities

227 PBAE-MPPs achieved robust transgene expression over at least 4 mo following a sing

228 ovides a useful molecular tool for directing transgene expression, particularly where up-regulation i

229 y types, including searching neurons against transgene expression patterns.

230 worked with transgenic sublines with drifted transgene expression patterns.

231 omoter sequences that can drive constitutive transgene expression patterns.

232 richment of recombinant cells independent of transgene expression, permitting the assessment of libra

233 Transgene expression persisted for weeks after implantat

234 nd suggest simple strategies to overcome the transgene expression problem in Chlamydomonas.

235 cus at disease-relevant levels and display a transgene expression profile that recapitulates that of

236 conventional nanocomplexes, or DNA-CN), and transgene expression reached beyond the tumor edge, wher

237 region of transgene plays a critical role in transgene expression regulation and silencing of transge

238 tic biology, specifically in genome editing, transgene expression regulation, and bioenergy crop engi

239 The ability to induce transgene expression repeatedly via administration of an

240 Epigenetic silencing of transgene expression represents a major obstacle for the

241 eta-actin promoters to drive low versus high transgene expression, respectively.

242 tion site-independent, copy number-dependent transgene expression restricted to macrophages, we demon

243 Transgene expression resulted in increased proteolytic a

244 mad7 bigenic (double transgenic) mice, Smad7 transgene expression reversed transforming growth factor

245 We developed a method for transgene expression specifically in mouse endometrium a

246 genic mouse model with doxycycline-inducible transgene expression specifically in the glomerular podo

247 However, rigorous assessment of transgene expression specificity in these animal models

248 curling virus, drives levels of constitutive transgene expression that are significantly higher than

249 luding diet, oxygen availability, Huntingtin transgene expression, the absence of macroautophagy (ATG

250 and endogenous protein detection, along with transgene expression, the less abundant BRD4 short isofo

251 Coupled with issues pertaining to transgene expression, the therapeutic landscape has unde

252 We wanted to determine the durability of transgene expression, the vector dose-response relations

253 , which was critical to achieving widespread transgene expression throughout orthotopic rat brain tum

254 (PBAE-MPPs) provided uniform and high-level transgene expression throughout the mouse lungs, superio

255 equires Cre-driver mouse lines that restrict transgene expression to a precisely defined cell populat

256 translation start site within exon 1 allowed transgene expression to be regulated by endogenous CD40L

257 bly in terms of both efficiency and reliable transgene expression to classic pronuclear microinjectio

258 pecific endogenous gene promoters to confine transgene expression to individual cell types (eg, ITGA2

259 ve previously shown that linking therapeutic transgene expression to induction of the chemokine CCL5/

260 binatorial specificity and stable, long-term transgene expression to isolate precisely defined neuron

261 uitry require Cre-driver lines that restrict transgene expression to precisely defined cell populatio

262 ated sequences could drive stable luciferase transgene expression to significantly higher levels than

263 However, reproducibly targeting transgene expression to specific fractions of a genetica

264 tory protein promoter, which largely limited transgene expression to the respiratory bronchioles.

265 onic plasmid (MIP) is an alternative, robust transgene expression vector for reprogramming.

266 Silencing of exogenous DNA can make transgene expression very inefficient.

267 Genetic removal of VIPR2 transgene expression via crossing with Drd1a-Cre BAC tra

268 Up to a fiftyfold increase in tumor transgene expression was achieved, without any observabl

269 muscle samples revealed that increased GLUT4 transgene expression was associated with decreased level

270 a vitreous component, in adenoviral-mediated transgene expression was examined.

271 Transgene expression was increased approximately 50-fold

272 hCD39 transgene expression was localized to the vascular endot

273 on by food intake), recombinant AAV-mediated transgene expression was markedly reduced, both in vitro

274 ndividuals harboring the transgene, and high transgene expression was observed in the blood of some P

275 However, transgene expression was poor, most likely due to endoso

276 Transgene expression was regulated by the pathogen-induc

277 xpression and abated over several weeks once transgene expression was suppressed.

278 Zap70 transgene expression was then ablated by withdrawal of d

279 se, which was associated with a reduction in transgene expression, was characterized more thoroughly

280 In order to achieve kidney-specific transgene expression, we tested direct hydrodynamic inje

281 Mice with low transgene expression were normal and mice with medium ex

282 sive stages of the mdx pathology achieved by transgene expression were paralleled by reduced expressi

283 nucleus and the SC are lost after 8 weeks of transgene expression, whereas those in the oculomotor, t

284 r development was prevented by forcing PDE7B transgene expression, while knockdown of PDE7B effective

285 Two mutations in nsP2 and nsP3 enhanced transgene expression, while three mutations in nsP3 regu

286 cation, or TRAP, combines cell type-specific transgene expression with affinity purification of trans

287 bly in terms of both efficiency and reliable transgene expression with classic pronuclear microinject

288 bly in terms of both efficiency and reliable transgene expression with classic pronuclear microinject

289 now examine the consequences of suppressing transgene expression with doxycycline on the NFT-associa

290 ed components, remote stimulation of insulin transgene expression with RF or a magnet lowers blood gl

291 reflect preferential promoter usage because transgene expression with scAAV9/MeCP2-green fluorescent

292 Transgene expression with the PBAE/PBAE-PEG blended nano

293 ed inflammatory response and more persistent transgene expression, with luciferase activity persistin

294 completed in a few minutes and allows stable transgene expression within a few days, at success rates

295 glycolysis also boosted EnAd replication and transgene expression within human tumor biopsies and in

296 knockout mice, was found in regions of high transgene expression within the cerebellum.

297 Of note, the transgene expression within the orthotopic tumor tissue

298 enes have been co-opted to produce selective transgene expression within this population.

299 itches with wide dynamic ranges that control transgene expression without the requirement of addition

300 We hypothesized that delaying initial transgene expression would improve long-term transgene e